No description available.

Call number: LD2668 .T4 1965 S379 / Master of Science

Submitted in partial fulfilment of the requirements for the Doctrate Degree in Technology: Food Technology - Durban University of Technology, 2013. / A pearl millet (Pennisetum glaucum) based instant beverage powder (PMIBP) was prepared from two different varieties of pearl millet (Agrigreen (AgG) and Babala (Ba)) by a combination of malting and extrusion cooking. The millet grains were germinated (30oC and 98% RH for 36 h), kilned (50oC for 48 h), cooled to room temperature, ground and stored in a chiller at 5oC until used. The raw and malted pearl millet grains were extruded under different parameters to accommodate the types of pre-treatment applied to the pearl millets. Combination processing of the pearl millet grain was achieved by extrusion of malted pearl millet of both varieties individually, and as a mixture of raw and malted pearl millet (50:50). The effect of the processing methods on the physical, functional, nutritional and biochemical properties of the raw and processed pearl millets varieties were evaluated. Combination processing led to a significant (p ≤ 0.05) decrease in total fat and total dietary fibre (TDF) (3.85 and 22.99 g/100 g, respectively) content of AgriGreen (AgG) extruded malted pearl millet (EMPM); a decrease in TDF (18.12 g/100 g) content of AgG extruded raw pearl millet-malted pearl millet mix (ERPMMPM). Combination processing led to a decrease in ash, total fat, total dietary fibre, Fe and Zn (1.76, 3.48, 14.26 g/100 g, 7.78 and 4.74 mg/100 g, respectively) content of Babala (Ba) EMPM. It also led to a significant (p ≤ 0.05) decrease in ash, total fat, TDF, Fe and Zn (1.88, 4.22, 21.71 g/100 g, 7.24 and 4.14 mg/100 g, respectively) content of Ba ERPMMPM. Regardless of the pearl millet variety, malting led to a significant (p ≤ 0.05) decrease in moisture, total, saturated, mono- and polyunsaturated fats, total dietary fibre iron, zinc and protein digestibility; a significant (p ≤ 0.05) increase in total carbohydrates, total phenolic content, antioxidant activity (TEAC) and water solubility index. Extrusion however, led to a significant (p ≤ 0.05) decrease in total dietary fibre, zinc, total phenolic content and protein digestibility; a significant (p ≤ 0.05) increase in ash, total, saturated and monounsaturated fats, total carbohydrates, iron, starch digestibility water absorption index (WAI) and water solubility index (WSI). The beverages of 10% total solids (8% pearl millet + 2% sugar for taste) prepared from the processed pearl millet were offered to an untrained consumer panel consisting of students and staff of the Cape Peninsula University of Technology, under similar sets of conditions in a sensory evaluation room at the Food Technology Department. The following characteristics of the products were rated and scored on a 9-point hedonic scale (1 – like extremely and 9 – dislike extremely): appearance, colour, aroma, flavour, mouth-feel and overall acceptability. In general, Ba RPM was rated 4 - like slightly, and AgG malted pearl millet (MPM) was rated 6 - dislike slightly and all other pearl millet samples from both varieties were rated 5 - neither like nor dislike.

published_or_final_version / Urban Planning / Master / Master of Science in Urban Planning

Patients that have undergone organ transplantation are life-long treated with immunosuppressant drugs and these have to be monitored regularly to get the desired effect of suppressing the immune system. To monitor the drug concentration normally a venous blood sample is collected at a clinic but the use of dried blood spots (DBS) as a matrix for drug monitoring for immunosuppressant drugs will make home sampling possible for this patient group. The aim of this study was to develop and validate a bioanalytical method for quantifying cyclosporine A and tacrolimus in dried blood spots. The method consist of punching out a 5 mm disc from a blood spot , followed by extracting the spot in a 96-well hydrophobic filter plate with 150 µL extraction solution containing internal standard (ascomycin and cyclosporine A d12) in a methanol water solution (80:20v/v%). The extract is then centrifuged through the filter plate down in a 96-deep well plate and injected on the LC-MS/MS, with an analysis time of 2.5min. The method will be validated in accordance with the guidelines set by the European Medicines Agency with additions specific to DBS. The method is not fully validated but will be in due time. The validated parameters show a robust and fast analysing method that has the prospects of being used for analysing DBS samples for patients and in the future can possibly be used by patients in home environment.

Master of Science / Department of Agricultural Economics / Arlo Biere / A major story in the recent history of US agriculture is the evolution and growth of the ethanol industry. A crucial factor in the profitability of an ethanol plant is the choice of its fixed location, as this has implications in the transportation costs associated with the acquisition of grain and sale of distiller’s grains. When the industry was in its infancy, where to locate, often, was based on strictly local factors. Primary considerations were local availability of grain and producer and community investment interests. Today, the ethanol industry is more mature and consolidated. As such, investment criteria have broadened from a localized to a total systems perspective. The focus of this study was to analyze construction, abandonment, and expansion of plant locations in ethanol producing regions, and the effects of regional transportation costs on the geographic growth of the industry. Comparison to previous research provided the basis to evaluate industry change. Current ethanol plant locations and their capacities were complied and compared with earlier data to identify plant exits, expansions and new construction. Aggregating those plant capacities by USDA crop reporting districts, feedstock consumption by biorefineries were calculated by crop reporting district, as was livestock feed demand from livestock numbers. Those data along with coarse grain production by crop reporting district were used to calculate excess feedgrain demand (supply) by region. Those regional data were used to construct linear programming network-flow models for the transportation of feedstock and for DDGS, respectively. Two models were used; the first was used minimize the interregional cost to transport feedstocks from excess supply regions to excess demand regions. The second was used to minimize the interregional cost to transport DDGS from excess supply regions to excess demand regions. These regional transportation costs were combined to find the total interregional transport by crop reporting district. Differences in such interregional transport costs affect the competitiveness of plants across crop reporting districts and should affect the strategic position of each plant location. Current plant locations and transportation cost results were compared with those from previous research and, with additional consideration to changes in production factors, provided further understanding of the recent growth and development of the ethanol industry.

No description available.

Call number: LD2668 .T4 1986 S36 / Master of Science / Horticulture, Forestry, and Recreation Resources

Master of Science / Department of Animal Sciences and Industry / Robert Goodband / Five experiments were conducted to determine the effects of sorghum dried distiller’s grains with solubles (DDGS) on nursery pig performance, finishing pig performance and meat quality, and lactating sow and litter performance. Experiment 1 established a nutrient database and evaluated the quality and consistency of five ethanol plants in the Western Plains region. Each sample was analyzed for AA, DM, CP, crude fiber, crude fat, ash, NDF, ADF, Ca, P, trace minerals, GE, and starch. In addition, DE, ME, and NE were calculated. Experiments 2 and 3 evaluated the effects of sorghum DDGS on nursery pig performance. In Exp. 2, pigs fed sorghum- or corn-based diets performed similarly; however, as sorghum DDGS increased ADG decreased (linear, P < 0.01). A DDGS×grain source interaction (quadratic, P = 0.03) was observed for G:F. In corn-based diets, G:F was identical for pigs fed 0 to 30% DDGS, but worsened in pigs fed 45% DDGS. In sorghum-based diets, G:F was best for those fed 0% DDGS, but worsened at higher levels. In Exp. 3, pigs fed corn- or sorghum-based diets performed similarly; however, pigs fed DDGS gained less (P < 0.03) than those fed basal diets. Experiment 4 evaluated the effects of sorghum DDGS in sorghum- or corn-based diets on finishing pig growth performance, carcass characteristics, and fat quality. Increasing sorghum DDGS reduced (linear; P < 0.01) ADG and increased (linear; P < 0.01) backfat IV. Pigs fed the sorghum-based diet with 30% sorghum DDGS had decreased backfat IV (P < 0.01) than those fed the sorghum-based diet with corn DDGS. Experiment 5 evaluated the effects of sorghum DDGS on lactating sow and litter performance. Overall, ADFI increased in corn-based diets when DDGS were added, but decreased in sorghum-based diets resulting in a tendency (P < 0.08) for a DDGS × grain source interaction. Pig weaning weights were lower (P < 0.06) for sows fed diets containing DDGS compared with those fed the basal diets. Adding sorghum DDGS reduced pig performance, and sow lactation performance; therefore, its inclusion in swine diets needs to be evaluated on an income over feed cost basis.

Many different bacteria have essential roles in the process ofrecycling organic waste, making them useful tools when it comes toestablishing artificial ecosystems, a key technology to master inthe expansion of human space travel.In order to further investigate bacteria growth conditions duringspace travel, the MOREBAC experiment was formulated. The objectivewas to design an experimental setup and develop measurementequipment with the capability of confirming successfulresuscitation of freeze-dried bacteria in space by measuringbacteria growth, on-board the student-built MIST-satellite.The experimental setup prototype consisted of an acrylic chipwherein the bacteria would be placed during experiments and anoptical measurements configuration using a photosensor with thepurpose of detecting bacteria cell growth. For experimentalenvironment monitoring, a temperature sensor and a pressure sensorwere calibrated.An Arduino Nano microcontroller was programmed to control allelectrical components during measurements. During the opticaldensity measurements blue dyed water and E.coli bacteria innutrition media were used as test samples.Provided varying blue dye or bacteria cell concentrations, in theform of dilution series and growth-over-time-series, the equipmentproved capable of producing measurements that indicate the opticaldensity of the test sample.Furthermore, a prototype experiment protocol simulating eventsthat will occur in the final experiment design, was implementedand was able to produce real-time monitoring graphs of optical,temperature and pressure measurements, as well as documentation ofall events and measurement data. / MOREBAC / MIST