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>Magister Scientiae - MSc / Drought is a major stress that affects the productivity of plants across the globe. The consequences of drought are posed to continue reducing agricultural productivity as world climate changes. This makes it imperative to improve tolerance of staple food crops to drought. Under drought stress, reactive oxygen species (ROS) accumulate, which may lead to oxidative damage of DNA, proteins and lipids.

Future climate projections suggest an increase in average temperature as well as a decrease in average winter rainfall across the south-west Australian floristic region (SWAFR). These adverse future climatic conditions will amplify the intensity and frequency of disturbance events such as drought and fire. Mediterranean forests within the SWAFR are prone to drought and fire disturbance and have acquired resilience through the selection of drought and fire tolerable species. However, shifts in the magnitude of these disturbance events could increase the recovery period required for recruitment, causing a shift in forest structure and decreasing the resilience of these ecosystems to future disturbances. In this study, we investigated above-ground biomass (AGB) accumulation of understorey plants at sites within the Northern Jarrah Forest (NJF) that have experienced different degrees of drought and fire intensity. We found that within a disturbance event, sites experiencing either more severe drought and fire intensities on average accumulated substantially more understorey AGB than sites subjected to both low drought and moderate fire intensities. This suggests that understorey species within the SWAFR gain a competitive advantage in high drought and fire severity conditions and are highly tolerant to drought. However, the increase in understorey AGB accumulation also suggests a shift in overall forest structure to more dense, compact, low-ground small stems, which is known to increase fire probability. An increase in fire probability shortens the time period between fire intervals and can detrimentally affect forest recovery, especially in drought conditions. Therefore, these changes may shift ecosystems within the SWAFR to a state of non-equilibrium and reduce resilience to future disturbance events.

The occurrence of water crises in many parts of the world raises the need to consider more efficient and sustainable consumption of water resources. As such, many cities have prioritised water demand management strategies, which are based on price and non-price mechanisms. The literature shows no consensus as to which of these measures are most effective for managing residential water demand. To understand the impact and effectiveness of these mechanisms, there is a need to understand how people respond to them. This requires understanding materials, meanings and competences (skills and know how) that people have, which constitute elements of social practice. In 2017 and 2018, the City of Cape Town (CoCT) ramped up their price and non-price mechanisms to encourage people to save water in response to a severe drought. These mechanisms included water restrictions, increased water tariffs, and the Day Zero communication campaign. However, little is known about how effective these measures were at encouraging people to save water. There is no clear documentation of how the public understands, interprets and incorporates these mechanisms into their own household water use practices. This study explores the impacts and effectiveness of the City of Cape Town’s price and non-price mechanisms on household water use practices during the water crisis. Using information obtained through semi-structured interviews with 20 individuals living in houses where they paid their water bills, a version of social practice theory is used as a lens to understand how respondents interpreted and responded to these mechanisms when it comes to residential water use practices. This allows for an assessment of which of the CoCT’s actions are more effective in achieving sustainable water use practices. Results show that price mechanisms (water tariffs) were considered to be ineffective and did not encourage people to save water. Non-price mechanisms (water restrictions and Day Zero communication campaign) were seen as having more impact on respondents, encouraging water conservation behaviour; especially when it comes to household indoor water use activities related to hygiene. Compared to other studies which have used estimates for the water demand function, this study conducted interviews directly with households on the impact of the mechanisms. This enabled this study to explain how and why household water use practices change in response to these measures. Such qualitative information is important and contributes to a field that often uses quantitative data to suggest whether price or non-price mechanisms are effective.

In many areas, over half of all diverted water is used for irrigation. Tree fruit crops use a lot of water, but water productivity can be increased using properly-timed precision water stress. In addition to water conservation, increases in water productivity arise from better fruit quality, increased storage life and reductions in pruning and maintenance. One major hurdle to applying precision water stress in orchards is the lack of a reliable, automated method of determining tree water status. However, the influence of physiological characteristics such as rootstock vigor on water productivity are also important. Selecting the most appropriate rootstocks and accurately determining the water status of orchard trees can increase water productivity. Research has shown that some rootstocks can more effectively extract water from soil. In this research, the response to water stress of three different Gisela tart cherry dwarfing rootstocks was compared using a weighing lysimeter system. Gisela 12 and Gisela 3 rootstocks recovered from drought stress more quickly and had higher trunk diameter growth rates than drought-stressed Gisela 5 rootstocks. Two potential methods of determining tree water status were also evaluated. Trunk hydration was measured using electromagnetic sensors and canopy temperature changes were detected using infrared radiometry. Electromagnetic techniques, including time domain reflectometry, can be used to determine the water content of wood. Until recently, the cost of this technology has inhibited its widespread use, but new affordable commercial electromagnetic soil moisture sensors have created renewed interest in this technique. In this research five different types of electromagnetic soil moisture sensors were inserted into the trunks of fruit trees and were monitored over two growing seasons. Maximizing exposure of waveguides to the sapwood increased the response of these sensors to changes in stem water potential. Infrared measurements of canopy temperature have successfully been used with field crops. However, the heterogeneity of orchard canopies makes this technique more difficult in orchards. Here, the efficacy of aiming radiometers at single trees versus at entire orchards was compared over multiple growing seasons. Neither single tree measurements nor whole orchard techniques produced a sufficiently robust signal to recommend them for general use.

Both salinity and drought stresses induce osmotic stress. Thus, cross-tolerance responses and mechanisms may occur in plants. The overall objectives of this study were to determine morphological and physiological responses and mechanisms of turfgrasses and woody species under salinity and drought stress conditions, and determine the relationship between drought and salinity tolerance ability in those species. Five turfgrass entries, ‘Gazelle’ and ‘Matador’ tall fescue (TF), ‘Midnight’ Kentucky bluegrass (KBG), PI368233 (Tolerant KBG), and PI372742 (Susceptible KBG), and three woody species, bigtooth maple (xeric-non saline), bigleaf maple (mesicnon saline) and Eucalyptus (mesic-saline) were compared. For the drought study, water was withheld in Chapter 2 while the dry down treatment was based on daily evapotranspiration (ET) in Chapters 5 and 6. For the salinity study, NaCl and CaCl2 in turfgrasses at electrical conductivity (EC) of 1, 6, 12, 18, and 30 dS m-1 (Chapter 3) and woody species at EC of 1, 3, 6, 9, and 12 dS m-1 (Chapter 4). Susceptible KBG was sensitive to s

Climate change is associated with altered environmental conditions and shifting mosaics of suitable habitats for organisms. Climate change in the form of drought can shift important lake shoreline habitats downslope, altering the lakes chemistry and habitat availability. Additionally, negative biological consequences can occur after a loss of submerged habitat along shorelines, hereafter littoral habitat. The objective of this study is to evaluate whether littoral habitat is lost (cobble, coarse woody habitat (fallen trees; CWH), and aquatic vegetation) under drought conditions across the United States. I used the National Lakes Assessment physical habitat data collected in summer 2012, when 75% of the U.S. experienced drought. I calculated the probability of cobble, CWH, and aquatic vegetation loss with lake level decline. I found cobble and CWH were highly vulnerable, where just 1 meter of lake level loss would result in nearly 100% habitat loss. Aquatic vegetation exhibited vulnerability but at a higher threshold. Multiyear drought will continue into the future with scientists estimating increases in drought frequency and severity, and we do not yet understand how or if aquatic animals will be resilient to a loss of littoral habitat. For example, previous research suggests food webs may be slow to recovery following littoral habitat loss. We must continue to evaluate the biological and environmental consequences of littoral habitat loss under drought conditions to successfully manage lakes and reservoirs into the future.

>Magister Scientiae - MSc / Agricultural practices are significantly affected by drought. Drought is one of the most important plant stresses, causing several physiological, morphological, biochemical, and molecular changes in plants. Drought stress is of great challenge for crop growth, development and yield. Zea mays (maize) is one of the important crops worldwide due to the nutritional profile and other uses such as human consumption, manufacturing and animal feed. Under unfavorable conditions, plants produce high amounts of reactive oxygen species (ROS). Excessive formation of ROS is harmful for plant survival and can induce cell death. Defense mechanisms activated in response to drought in plants include antioxidant enzyme activity and proline accumulation. There is evidence of the use of nitric oxide (NO) donors and hydrogen peroxide (H2O2) at low concentrations to enhance the activity of antioxidant enzymes in stressed plants. Hence, the aim of the study is to examine the role of NO and H2O2 in regulation of antioxidant enzyme activity in maize subjected to water deficit. / 2023

Drought and salt stress are the main global factors that reduce the average yield of most major crops. In order to meet global demands, we will need to double food production by 2050 (Tilman, Balzer, Hill, & Befort, 2011). Plant growth-promoting bacteria (PGPB) are a group of bacteria that alleviate the harmful effects of abiotic stresses such as salt, heat and drought stress on plants and decrease the global dependence on hazardous agricultural chemicals. We identified that beneficial microbes isolated from desert plants (indigfera argentea) from Jizan region, in 2012 enhance the tolerance of a variety of crop plants to drought and salt stresses under laboratory conditions and in field trials. We analyzed the interaction of these bacteria with the plants by genetic, biochemical and imaging techniques. The goal of this dissertation is to ultimately improve our understanding of the mechanisms of drought and salt stress tolerance conferred by beneficial microbes that can be used as a sustainable solution for plants and crops in degrading lands (deserts) and land affected by abiotic stresses. Outlines how each of chapter of this dissertation will contribute to the discovery of novel drought and salt stress tolerance strategies using a desert-specific bacterial endophyte.

京都大学 / 0048 / 新制・課程博士 / 博士(農学) / 甲第20006号 / 農博第2190号 / 新制||農||1045(附属図書館) / 学位論文||H28||N5015(農学部図書室) / 33102 / 京都大学大学院農学研究科地域環境科学専攻 / (主査)教授 藤原 正幸, 教授 村上 章, 准教授 宇波 耕一 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DFAM