Thesis (M.Sc. (Animal production)) --University of Limpopo, 2011 / A study was conducted to examine the effect of protein level of the diet of Naked neck hens on egg production, hatchability and chick productivity. The first part of the experiment determined the effect of protein level on feed intake, number of eggs produced, egg weight, egg white nitrogen content, egg yolk nitrogen content, egg hatchability and chick hatch-weight. A total of 25 hens aged 30 weeks were confined in cages and fed diets differing in protein content. A complete randomized design, comprising of diets differing in protein levels (P12.94%, P13.94%, P14.38%, P15.75% and P18.13% CP), replicated five times with one hen in each replicate was used. Semen was collected from one cock to fertilize all hens. Protein level had an effect on (P<0.05) feed intake and egg white nitrogen content. However, protein level of the diet of hens had no effect(P>0.05) on the number of eggs produced, egg white nitrogen content, egg yolk,egg weight, egg hatchability and chick hatch-weight. Quadratic analyses indicated that feed intake, number of eggs produced, egg weight, egg white nitrogen content, egg yolk nitrogen content, hatchability and chick hatch-weight were optimized at different protein levels of 14.7 (r2 = 0. 623),14.9 (r2 = 0.568),13.9 (r2 = 0.094), 18.2 (r2 = 0.563), 15.1(r2 = 0.424), 15.9 (r2 = 0.451) and 15.9 %(r2 = 0.898) , respectively. The second part of the experiment determined the effect of protein level of hens on productivity of their progenies from a day old up to 13 weeks of age. A total of 95 chicks hatched from the first part of the study were assigned to 20 floor pens according to the number of chicks hatched. Chicks had an average initial live weight of 35 ± 2 g per bird. The chicks were fed the same commercial grower diet and fresh water ad libitum up to seven weeks of age. Protein level of the diet of Naked neck hens had an effect (P<0.05) on feed intake, live weight and feed conversion ratio of chicks from a day old up to seven weeks of age. However, protein level of the diet of Naked neck hens had no effect (P>0.05) on growth rate and mortality of chickens from a day old to seven weeks of age. Feed intake, feed conversion ratio, growth rate and mortality of chickens were optimized atdifferent protein levels of 15.8 (r2 = 0.298), 16.1 (r2 = 0.236), 16.1 (r2 = 0.077) and 14.3 % (r2 = 0.617), respectively. Protein level of the diet of Naked neck hens had no effect (P>0.05) on feed intake, growth rate and feed conversion ratio of both their male and female progenies between eight and 13 weeks of age. Protein levels of the diet of hens had an effect (P<0.05) live weight of their male progenies and carcass weights of both their male and female progenies at 13 weeks of age. However, the level of protein of the diet of hens had no effect (P>0.05) on live weight of their male progenies, and breast meat yield, breast meat nitrogen content and fat pad weight of both their male and female progenies at 13 weeks of age. Live weight,breast meat yield, breast meat nitrogen content and fat pad weight of their male progenies at 13 weeks of age were optimized at different protein levels of the diets of hens of 18.9 (r2 = 0.666), 15.6 (r2 = 0.081), 15.4 (r2 = 0.786) and 17.7 %(r2 = 0.775), respectively. Similarly, live weight, carcass weight, breast meat yield, breast meat nitrogen content and fat pad weight of their female progenies at 13 weeks of age were optimized at different protein levels of 15.7 (r2 = 0.294),15.4 (r2 = 0.180), 15.8 (r2 = 0.059), 15.1 (r2 = 0.882) and 16.1 % (r2 = 0.405), respectively. It is concluded that protein level of the diet of the hen may affect its productivity and that of its progeny. However, the calculated protein levels for optimum productivity will depend on the production parameter in question. / National Research Foundation

Thesis (M.Dev.) --University of Limpopo, 2011 / Poverty among communities is not a static condition. There are many factors which contribute to its causes and depth. In South Africa, the economic gaps imposed by the previous apartheid government aggravated economic inequalities and caused considerable disparities, which resulted in the high unemployment rates. This led to a call for pro-active initiatives by post 1994 democratic government to remedy the living condition of the previously disadvantaged people. As part of its poverty relief drive, the South African government has embarked on a series of developmental initiatives to bring services to the poor and to reduce enormous prevailing backlogs, with the aim of increasing community participation and improving the lives of the poor. This study evaluates the egg layers project as a poverty alleviation effort within Blouberg Municipality of the Limpopo Province to respond to the prevailing poverty and underdevelopment among the poor communities. This study revealed from the observations, interviews conducted and questionnaires administrated to sampled beneficiaries of Blouberg Municipality who faces poverty. The study formulated strategies and made recommendations regarding the impact and implementation of the egg layers on poor people.

Corrigenda inserted behind title page. / Copies of author's previously published articles inserted at end. / Includes bibliographical references (leaves 193-210). / xxii, 210 leaves : ill. (some col.) ; 30 cm. / Title page, contents and abstract only. The complete thesis in print form is available from the University Library. / Investigates whether estrogen modulates the proteins associated with calcium transport (eg calcium binding protein, calbindin D28k) in the intestinal tissue during sexual maturation of laying hens. Studies the temporal and spatial expression of estrogen receptors (ER) and calcium binding protein in jejunal tissue. Findings indicated the potential of estrogen to induce calbindin D28K in the intestinal tissue, from which it can be concluded that estrogen may increase calcium absorption and consequently calcium retention. Shows that the amount of estrogen normally present in the reproductive system is sufficient for the normal capacity of egg and eggshell production. Egg production and shell quality are significantly reduced by phytoestrogen. It is recommended that the concentration of phytoestrogen in poultry diets be investigated in more detail as it may act as an anti-nutritive factor and affect metabolism and productivity. / Thesis (Ph.D.)--University of Adelaide, Dept. of Animal Science, 1999

Omega-3 polyunsaturated fatty acids (n-3 PUFA) enriched eggs are an increasingly important contributor to the egg industry due to the health benefits of n-3 PUFA. During storage and cooking, n-3 PUFA could undergo oxidation and further cause cholesterol oxidation. This study examined stability of n-3 PUFA enriched eggs fortified with antioxidants (vitamin E or organic Selenium [Sel-Plex] or both) following storage and cooking. Total n-3 PUFA was not affected by cooking and storage, although long chain n-3 PUFA concentration decreased. Overall, both vitamin E and Sel-Plex decreased oxidation of yolk lipids and cholesterol. Cooking increased the lipid and cholesterol oxidation. Four weeks of storage did not affect the cholesterol oxidation products (COPs) content, but increased lipid oxidation. It is possible to make the n-3 PUFA in enriched eggs more stable with dietary antioxidants. / Food Science and Technology

I tested a theoretical model proposing that anemia favors transmission of blood-borne parasites to vectors by accelerating the blood-feeding rate. Using Aedes aegypti, the yellow fever mosquito, initially, I was not able to confirm this phenomenon either in an artificial or a live system; anemia did not correlate with blood-feeding rate, time or volume. I then analyzed the feeding rate over different time intervals to examine the possibility that inconsistent feeding rates masked the expected results; a comparison of the blood-feeding rate at one particular time period supports the theory. I then continued investigating the influence of anemia on egg production of mosquitoes and found that anemia has negative influence on vector's fecundity. I conclude that although anemia has opposite influences on mosquitoes in blood-feeding rate and egg production, they can get, at least under certain conditions, benefit from blood-feeding on anemic hosts. / Graduation date: 1992

Thesis (M.S.)--Mississippi State University. Department of Poultry Sciences. / Title from title screen. Includes bibliographical references.

The Drosophila dorsal group gene pipe provides the crucial link that transmits dorsal-ventral (DV) polarity information from the ovary to the embryo. Females homozygous for mutations in pipe produce dorsalized embryos. pipe encodes ten protein isoforms with amino acid sequence similarity to vertebrate glycosaminoglycan 2-O-sulfotransferases, suggesting that Pipe functions by modifying a carbohydrate-bearing molecule that controls embryonic DV patterning. Two major components of my project have been to examine the functional specificities of different Pipe isoforms and to identify Pipe's enzymatic substrate and learn how it participates in DV pattern formation. I have used two approaches to investigate whether the various Pipe isoforms share the same functional specificities. In one approach, I expressed each isoform in the follicle cells and found that the expression of only one of them was able to rescue the pipe mutant phenotype or ventralize progeny embryos. In a second set of transgenic studies, three of the other isoforms were individually shown to restore the production of a pipe-dependent sulfated epitope when expressed in the salivary glands of otherwise pipe null mutant embryos. These data indicate that distinct functional specificities are associated with the various Pipe protein isoforms. In addition, these studies allowed me to determine that embryos from females lacking endogenous pipe expression nevertheless retain polarity along their dorsal-ventral axis, suggesting the existence of a second polarizing signal in addition to the ventral transcription of pipe. To identify Pipe’s substrate, I developed a technique for metabolic labeling which enabled me to identify a molecule exhibiting Pipe-dependent sulfation. This molecule was identified as the protein Vitelline Membrane-Like (VML), a putative component of the vitelline membrane layer of the eggshell. The involvement of VML in dorsalventral patterning was demonstrated on the basis of the enhancing effects of a vml mutation on the severity of dorsalization of embryos from females of a sensitized genetic background. Thus, VML represents a bona fide substrate of Pipe that participates in the establishment of dorsal-ventral polarity. In these studies I was also able to show Pipedependent sulfation of other vitelline membrane components which may also influence embryonic dorsal-ventral patterning. / text

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