[Truncated abstract] Biogeochemical processes in seagrass sediments influence growth and nutrition of seagrasses. This thesis investigates the below-ground interactions between biotic and abiotic factors that influence seagrass nutrition and growth, with focus on a small species of seagrass, Halophila ovalis (R. Br.) Hook ƒ., from the Swan River Estuary, Western Australia. Seagrass showed significantly lower growth and an increase in leaf nitrogen and phosphorus concentrations with increased organic matter loading. With maximal light reduction, lower growth rates and average leaf weights were observed, and leaf nitrogen and phosphorus concentrations were higher. Light reduction was also shown to increase bioavailability of inorganic nutrients within porewater of seagrass sediment . . . Sulphide was hypothesised to have an inhibitory effect on nutrient uptake of Halophila ovalis. Below-ground sulphide inhibits the photosynthetic efficiency of photosystem II at sulphide concentrations greater than 1 mM. Sulphide exposure enhanced phosphate uptake, with no significant effect on ammonium uptake of H. ovalis. This thesis demonstrates that biogeochemical processes both constrain the potential growth of seagrasses and influence the nutrient status of seagrass tissue. Consideration of the influence of sulphide stress on seagrasses is likely to be particularly important for anthropogenically influenced aquatic systems, where inputs of organic matter are enriched relative to pristine ecosystems. A better understanding of biogeochemical processes will allow researchers to predict how future changes in sediment chemistry will influence seagrass meadows.

Este trabalho é parte de um projeto mais amplo intitulado \" Tipologia de Reservatórios do Estado de São Paulo\", financiado pela FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) que compreendeu o estúdo de 52 reservatórios. Neste estudo, quatro amostragens foram realizadas em dez reservatórios, durante o ano de 1979. Sete destes ambientes pertencem à Bacia do Tietê: Americana, Atibainha, Cabuçú, Cachoeira, Pedro Beicht, Ribeirão do Campo e Taiaçupeba; e três à bacia do Rio Paraíba do Sul: Jaguari, Paraibuna e Santa Branca. Em cada reservatório, foi estabelecida uma estação de coleta, exceto em Paraibuna, onde foram estabelecidas três estações. A produção primária medida pelo método do 14C, foi determinada de forma a se obter um perfil vertical na zona eufótica, assim como as concentrações de clorofila-a . Os dados físico-químicos sobre penetração de luz, temperatura, condutividade, oxigênio dissolvido, pH, alcainidade, formas de CO2, nutrientes e íons em solução foram obtidos ao longo da coluna de água, principalmente na região da zona eufótica. Os resultados obtidos permitiram a identificação de dois padrões de circulação: monomíctico quente e polimíctico. As medidas de produção primaria mostraram que, de modo geral, os reservatórios são pouco ou medianamente produtivos, (41,4 a 285,8 mgC.m-2 .dia-1 ) o que decorre do fato de estarem situados em terrenos pobres, na parte alta das bacias de drenagem. Isto se reflete nos baixos valores de condutividade, alcalinidade e na pobreza especialmente de fosfatos. Em alguns destes reservatórios, também a extensão da zona eufôtica, diminuindo a produtividade. O Reservatório de Americana foi uma exceção por tratar-se de um ambiente que vem sofrendo eutrofização cultural. Em conseqüência, sua produtividade média atingiu 768,2 mgC. m-2 dia-1 . Os valores de eficiência fotossintética observados foram baixos, (0,003 a 0,308%) semelhantes à grande maioria dos ambientes aquáticos. O nanoplâncton foi o principal responsável pela produção primária, o que provavelmente decorre da maior relação superfície/volume apresentada por estes organismos e que permite a melhor exploração de ambientes oligotróficos. Aparentemente, o fósforo é o principal nutriente limitante na maioria dos ambientes, como sugerida pelas baixas concentrações de fosfato dissolvido e pelas razões extremamente altas de N:P obtidas em diversos reservatórios, particularmente no inverno. / The present work is part of a 1argeT project entited \"Typo1ogy of Reservoirs of the State of São Paulo\". It was sponsored by FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo - State Foundation for Research Support), and included the study of 52 reservoirs. For the present study, ten reservois were sampled four times during 1979. Seven of these belong to the Tietê River basin: Americana, Atibainha, Cabuçu, Cachoeira, Pedro Beicht, Ribeirão do Campo and Taiaçupeba; and three to the Paraíba do Sul River basin: Jaguari, Paraibuna and Santa Branca. One samp1ing station was selected for each reservoir except Paraibuna, where three stations were chosen. Primary production, measured by the 14C method, as wel1 as concentrations of ch1orophyll-a were determined in selected depths in order to provide a vertical profile of these variables in the euphotic zone. Physico-chemica1 data such as ligth penetration, temperature, electric conductivity, dissolved oxygen, pH, alkalinity, forms of C02, nutrients and ions in solution were obtained along the water column, especially in the euphotic zone. The resu1ts permitted the detection of two circulation patterns: warm monomictic and po1ymictic. In a general way, primary production levels were from low to medium (41.4 to 285.8 mg C.m-2 .day-1), a consequence of most the reservoirs being located in the upper parts of their drainage basins, characterized by poor soils. This is reflected in low va1ues of conductivity, alkalinity and, among the nutrients, of phosphates. On the other hand, in some of the reservoirs low ligth penetration values reduced the thickeness of the euphotic zone, also lawering productivity. Americana Reservoir, suffering a process of cultural eutrophication, was an exception, its productivity averaging 768.2 mg C.m-2 . day-1 . Observed vaIues of photosynthetic efficiency were low (0.003 to 0.308 per cent) , as in the great majority of aquatic environments. The nanoplankton were responsible for most of the primary production, probably due to their higher surface volume ratios, allowing them to exploít more easily oligotrophic environments. Apparently phosphorus is the chief limiting nutrient in most reservoírs, as suggested by the low values of dissolved phosphates and by the very high N:P ratios observed in several of the reservoirs, particularly in winter.

The main aim of the project is to investigate the feasibility of artificial recharge of the Bushmans River Mouth Aquifer in the Eastern Cape. The suggested method of recharge is through natural infiltration through a series of wetlands in the back dune area. The proposed site was the Klipfontein Vlei which is located just north of the Bushmans River Mouth. Artificial recharge is defined as augmenting the natural movement of surface water into underground formations by some method of construction, the spreading of water, or by artificially changing natural conditions. A variety of methods have been developed, including water spreading, recharging through pits and wells, and pumping to induce recharge from surface water bodies. The choice of a specific method is controlled by local topography, geology and soil conditions, and the quantity of water to be recharged and ultimate water usage. In special circumstances, land value, water quality, and climate play an important role. Artificial recharge is not a foreign concept to South Africa and this project is to test its feasibility in a back dune wetland area. Augering done in the study area revealed the dominance of sandy loam in the upper layers of the pans, loamy sand in the middle levels and the presence of some sand lenses in the lower reaches. Particle size analysis done by sieving and hydrometer test, showed a dominant 3f fraction. There was variation in modes in pan 1 from unimodal to bimodal to trimodal. Pan 2 showed no variation as all samples were unimodal and moderately well sorted. Infiltration tests were done by a double ring infiltrometer. These results showed that the infiltration rates of the pans became stable after 15 to 20 minutes, thus indicating that the soil had reached its saturation point. The resistivity surveys revealed a stepped water table. The inclination of the water is controlled by the predominately argillaceous horizons of the Bokkeveld Group. Based on the above results, it is recommended that the Klipfontein Vlei be used as an artificial recharge site.

The release of nutrients (nitrogen and phosphorus) via land clearing, application of fertilisers, discharge of human waste and combustion of fossil fuels, is one of the most harmful effects of anthropogenic stresses on aquatic environments. This study investigated the use of reeds as indicators of nutrient input to estuaries. Small estuaries such as the oligotrophic East Kleinemonde where the research was mostly conducted are sensitive to nutrient enrichment from septic tanks, stormwater runoff and fertiliser application to lawns in close proximity to the estuary. Nutrient concentrations were sampled at different positions, in the groundwater, at the water’s edge, in the reed bed and in the main estuary channel at five sites in the East Kleinemonde Estuary. The NH4+ and SRP concentrations showed a definite trend as concentrations decreased from the groundwater or water’s edge into the main estuary channel. Groundwater introduced nutrients to the estuary which were then taken up by the fringing reeds (Phragmites australis (Cavinelles) Trinius ex Steudel). The water column nutrients were either below detectable limits or in very low concentrations. Low TOxN concentrations were consistently found at the different sites and probably resulted from P. australis assimilating N-TOxN for growth more efficiently than N- NH4+. The roots, rhizomes, stems and leaves of the reeds were measured for δ15N as an indicator of nutrient enrichment as nitrogen stable isotope analysis of plant tissue is an effective method for assessing and monitoring septic tank and other anthropogenic inputs. All plant parts for the three sampling sessions at Site 1 (mouth region) in the East Kleinemonde Estuary had significantly higher δ15N signatures (~20 percent) than the leaves at Site 5 (upper reaches). These differences were related to the surrounding land-use of the East Kleinemonde catchment, where the lower part of the estuary has moderate to low residential development and the upper reaches are utilized mainly for livestock farming. The high δ15N signatures at Site 1 were attributed to septic tank wastewater and stormwater run-off entering the estuary. Nutrient enrichment also influenced the morphology of the reeds. Reed biomass, height and stem density was significantly higher at Site 1 compared to Site 5. Similar studies were conducted in August 2008 in the Sundays Estuary and October 2008 in the nearby Mtati and Mpekweni estuaries to see if the results were similar. iii The same patterns were found where the groundwater NH4+ and SRP concentrations were higher than the estuary channel in the Mtati and Mpekweni estuaries. In the Mpekweni and Sundays estuaries Phragmites australis leaves had high δ15N signatures (10 and 11 percent) similar to that of Site 1 in the East Kleinemonde Estuary. Lower signatures (-2 to +4 percent) were found in the Mtati Estuary. This sampling site was sheltered by a bridge with a steep slope and the concentrations were similar to Site 3 and 5 in the East Kleinemonde Estuary where the derived nutrient was the result of stormwater runoff or fertilisers. Density and biomass of reeds in the Sundays and East Kleinemonde (Site 1) estuaries were similar and this could be related to different nutrient sources, agricultural return flow and septic tank input respectively. Reeds in the Sundays Estuary were significantly taller than in the other estuaries which showed that factors such as shelter and salinity also influence reed growth and therefore morphology alone would not be a good indicator of nutrient enrichment. Indicators of nutrient enrichment are important as the status of temporarily open / closed estuaries in South Africa is threatened by deteriorating water quality. Measurements of the water column may provide an inaccurate assessment of water quality whereas macrophytes are outstanding potential indicators of nutrient enrichment as they are widely distributed, abundant and long-lived. The findings from this study indicate that δ15N concentrations in reeds can be used to indicate nutrient loading.

In South Africa mangrove forests are located in estuaries from Kosi Bay in KwaZulu-Natal (KZN) to Nahoon Estuary in the Eastern Cape. The aims of this study were to determine the present state of mangroves in KwaZulu-Natal, by assessing the current population structure, the changes in cover over time and associated anthropogenic pressures. A second objective of this study was to determine the effect of harvesting on the population structure and sediment characteristics in the Mngazana mangrove forest. To determine if harvesting was sustainable at Mngazana Estuary; the growth and mortality rates and associated growth conditions were measured. Finally by using population modelling sustainable harvesting limits were determined by predicting the change in population structure over time. The study focussed on the KwaZulu-Natal province as a fairly recent study addressed mangrove distribution and status in the Eastern Cape Province. A historical assessment of all mangroves forests in KwaZulu-Natal (KZN) revealed that the potential threats to mangroves in South Africa include; wood harvesting, altered water flow patterns coupled with salinity changes, prolonged closed-mouth conditions and subsequent changes to the intertidal habitat. As a result mangroves were completely lost from eleven estuaries in KZN between 1982 and 1999 and a further two estuaries by 2006. Mangroves only occurred in those estuaries where the mouth was open for more than 56 percent of the time with the exception of St Lucia, where the mouth has been closed for longer but the mangrove communities have persisted because the roots of the trees were not submerged. All mangrove forests in KZN were regenerating in terms of population structure as they had reverse J-shaped population curves as well as high adult: seedling ratios. Kosi Bay and Mhlathuze Estuary were two of the larger forests that showed signs of harvesting (presence of tree or branch stumps), but the greatest threat to smaller estuaries seems to be altered water flow patterns due to freshwater abstraction in the catchments and the change of land use from natural vegetation to sugar-cane plantations. These threats affect the hydrology of estuaries and the sediment characteristics (particle size, redox, pH, salinity, temperature) of the mangrove forests. The environmental conditions under which the mangrove forests currently exist were determined for five species. Lumnitzera racemosa and Ceriops tagal exhibited a narrow range of conditions as these species are only found at Kosi Bay, while Avicennia marina, Bruguiera gymnorrhiza and Rhizophora mucronata were found to exist under a wider range of conditions. The growth rate and response to environmental conditions of the three dominant species were important to determine as these species are impacted by harvesting. Mangrove growth rates were measured at Mngazana Estuary in the Eastern Cape, the third largest mangrove forest in South Africa. Areas of this estuary where mangroves harvesting has occurred, show significant differences in sediment characteristics as well as changes in population structure in harvested compared to non harvested sites. The growth rate (in terms of height) of Avicennia marina individuals increased from seedlings (0.31 cm month-1) to adults (1.2 cm month-1), while the growth of Bruguiera gymnorrhiza stabilised from a height of 150 cm at 0.65 cm month-1. The growth of Rhizophora mucronata peaked at 0.72 cm month-1 (height 151-250 cm) and then decreased to 0.4 cm month-1 for taller individuals. Increases in diameter at breast height (DBH) ranged between 0.7 and 2.3 mm month-1 for all species. Some environmental variables were found to be important drivers of growth and mortality of individuals less then 150 cm. A decrease in sediment pH significantly increased the mortality of Avicennia marina seedlings (0-50 cm) (r = - 0.71, p<0.05) and significantly decreased the growth of Rhizophora mucronata and Bruguiera gymnorrhiza seedlings (r = -0.8, r = 0.52 – p < 0.05 respectively). At Mngazana Estuary, mortality of this species showed a positive correlation with sediment moisture content indicating that this species prefers drier conditions. The density of Rhizophora mucronata was significantly correlated to porewater temperature in Northern KZN as was the growth of adult (>300 cm) Rhizophora trees at Mngazana Estuary. Mortality of Avicennia marina individuals (51-150 cm) was related to tree density indicating intraspecific competition and self thinning. Selective harvesting of particular size classes of Rhizophora mucronata was recorded when comparing length of harvested poles (~301 cm) and the size class distribution of individuals. Taking into account the differences in growth rate for each size class for this species it will take approximately 13 years to attain a height of 390 cm which is the height at which trees are selected for harvesting at this estuary. This is 2.6 times slower than those individuals growing in Kenya. The feasibility of harvesting is dependent on the growth rate of younger size classes to replace harvested trees as well as the rate of natural recruitment feeding into the population. Different harvesting intensity scenarios tested within a matrix model framework showed that limits should be set at 5 percent trees ha-1 year-1 to maintain seedling density at > 5 000 ha-1 for R. mucronata. However harvesting of Bruguiera gymnorrhiza should be stopped due to the low density of this species at Mngazana Estuary. Harvesting of the tallest trees of Avicennia marina can be maintained at levels less than 10 percent ha-1 year-1. Effective management of mangrove forests in South African is important to maintain the current state, function and diversity of these ecosystems. Management recommendations should begin with determining the freshwater requirements of the estuaries to maintain the mouth dynamics and biotic communities and deter the harvesting of (whole) adult trees particularly those species that do not coppice. Further management is needed to ensure that forests are cleared of pollutants (plastic and industrial), and any further developments near the mangroves should be minimized.

Galerucella pusilla and G. calmariensis have provided successful biological control of purple loosestrife (Lythrum salicaria L.) in non-tidal areas but only marginal control in areas of tidal influence. While a previous study identified mechanical scour by tidal waters as the main cause of establishment failure, purple loosestrife stem density explained more than 80% of the variability in presence and absence of Galerucella at my study sites in the Columbia River Estuary. A logistic regression model using purple loosestrife stem density, elevation, and their interaction as predictors accurately predicted 92.5% of Galerucella presence or absence observations of a test data set (n= 201). Field data also identified a critical threshold of approximately 32 purple loosestrife stems/m2 , above which Galerucella were present 100% of the time at the release sites.

Estuaries are dynamic environments that are strongly affected by natural variability, as well as direct and indirect anthropogenic impacts. A better understanding of the drivers of carbon fluxes and biogeochemical variability in estuarine systems is needed, particularly with the increasing threat of ocean acidification. Morro Bay in Central California is a small nationally protected estuary, with seasonally low freshwater inputs. Since 2007, the bay has experienced a significant loss of native seagrass, Zostera marina, which is an important component of the marine ecosystem. Because seagrass photosynthesis decreases carbon dioxide and increases oxygen in the water column, the loss of seagrass has the potential to substantially change short-term carbonate chemistry and long-term carbon fluxes of an estuary. The spatial variability of carbonate chemistry was measured in Morro Bay using ship-board surveys during the low-inflow summer season and measured the temporal variability by collecting samples close to the shore from July to November. Discrete samples show an increase in total alkalinity and dissolved inorganic carbon in the mid and back bay regions, historically dominated by seagrass. Slightly lower total alkalinity and dissolved inorganic carbon were observed in the Fall season compared to the low-inflow Summer season. Analysis of the relative modification of alkalinity and dissolved inorganic carbon, paired with salinity and temperature data, contributes to an understanding of the drivers of the observed carbonate variability. This understanding may provide clues to the causes and effects of observed changes to the bay with seagrass loss. More broadly, it will inform the vulnerability of other low-inflow estuaries to future acidification and highlight the role seagrasses play in mitigating local acidification.

No description available.

[Truncated abstract] The expansion of human populations in coastal land margins has resulted in major modifcations to estuarine ecosystems. The use of numerical models as predictive tools for assessing remediation strategies is increasing. However, parameterisation of physical processes, developed mainly through field investigations, is necessary for these models to be reliable and effective management tools. The physical processes in micro–tidal diurnal tidal systems are relatively unknown and the current study examines field measurements obtained from the upper Swan River estuary (Western Australia), a diurnal, partially mixed system during the summer when the freshwater discharge is negligible. The aims of the study were to characterise, temporally and spatially, the dominant physical processes and associated sediment resuspension. Variability at three dominant time-scales were examined: 1) sub–tidal oscillations (∼5 to 10 days) resulting from local and remote forcing; 2) tidal (∼ 24 hours) due to astronomical forcing; and 3) intra-tidal (∼2 to 3 hours) resulting from the interaction between tidal constituents. Circulation in estuaries is widely accepted in the literature to be dominated, in varying proportions, by tidal range, freshwater discharge and gravitational circulation. In the upper Swan River estuary sub–tidal oscillations were responsible for the largest upstream displacement of the salt wedge in the absence of freshwater discharge. Moreover, these sub–tidal fluctuations in water level modified the ‘classic’ estuarine circulation. The dynamics of diurnal tides are largely controlled by the tropic month, which oscillates at a slightly different period to the lunar month, resulting in the spring–neap tidal cycle to be sometimes different from syzygy. The phase lag between the diurnal (O1 + K1) and semi-diurnal (M2 + S2) constituents, at the seasonal time scale cause the maximum tidal range to be near the solstice. Over a 24–hour tidal cycle this phase lag is manifested as an intra–tidal oscillation that occurs on the flood tide. Turbidity events that last ∼1 to 2 hours occur during the intra–tidal oscillation, but are not related to maximum shear stress predicted from the mean flow characteristics. The increases in turbidity during the intra–tidal oscillation is, however, correlated with the near–bed Reynolds fluxes. During the intra–tidal oscillation advection opposes the estuarine circulation in the near–bed region, promoting vertical shear that results in destratifcation of the water column. The turbulent mixing generated at the interface and in the near–bed region coincide with resuspension events. Similar turbidity data have often been disregarded and documented as being ‘spikes’ based on the premise that the mean flow was below a critical level to resuspend sediment. Resuspension events were not simply related to mean processes and may be controlled by turbulent instabilities generated when tidal currents reverse during an intra-tidal oscillation

[Truncated abstract] The Swan River is a highly seasonal estuary in the south-west of Western Australia. Salinity may vary from fresh to marine at various times throughout the estuary, depending mostly on the intensity of freshwater discharge. There are occasional problematic dinoflagellate blooms which have spurred investigation of the dynamics of the phytoplankton community. The objective of this research was to examine how phytoplankton biomass and species' successions are influenced by the multiple variables in the aquatic ecosystem, and, if possible, to determine the dominant factors ... Comparisons of phytoplankton nutrient limitation simulations with experimental observations from field bioassays require further investigation, but reinforce findings that nutrients may only limit phytoplankton biomass when there is a convergence of favourable hydrological and hydrodynamic conditions. The Swan River estuary has undergone substantial hydrological modifications from pre-European settlement. Land clearing has increased freshwater discharge up to 5- fold, while weirs and reservoirs for water supply have mitigated this increase and reduced the duration of discharge to the estuary. Nutrient loads have increased approximately 20-fold from pre-European levels. The individual and collective impacts of these hydrological changes on the Swan River estuary were examined using the hydrodynamic-ecological numerical model. The simulation results indicate that despite increased hydraulic flushing and reduced residence times, increases in nutrient loads are the dominant perturbation, producing increases in the frequency and biomass of blooms by both estuarine and freshwater phytoplankton. By comparison, changes in salinity associated with altered seasonal freshwater discharge have a limited impact on phytoplankton dynamics. Reductions of nutrient inputs into the Swan River estuary from its catchment will provide a long-term improvement in water quality but manipulations of freshwater discharge have the potential to provide a provisional short-term remediation measure allowing at least partial control of phytoplankton bloom potential and eutrophication.