Global ETD Search

41	Gene expression of tendon markers in mesenchymal stromal cells derived from different sources Burk, Janina, Gittel, Claudia, Heller, Sandra, Pfeiffer, Bastian, Paebst, Felicitas, Ahrberg, Annette B., Brehm, Walter 15 December 2014 (has links) (PDF) Background: Multipotent mesenchymal stromal cells (MSC) can be recovered from a variety of tissues in the body. Yet, their functional properties were shown to vary depending on tissue origin. While MSC have emerged as a favoured cell type for tendon regenerative therapies, very little is known about the influence of the MSC source on their properties relevant to tendon regeneration. The aim of this study was to assess and compare the expression of tendon extracellular matrix proteins and tendon differentiation markers in MSC derived from different sources as well as in native tendon tissue. MSC isolated from equine bone marrow, adipose tissue, umbilical cord tissue, umbilical cord blood and tendon tissue were characterized and then subjected to mRNA analysis by real-time polymerase chain reaction. Results: MSC derived from adipose tissue displayed the highest expression of collagen 1A2, collagen 3A1 and decorin compared to MSC from all other sources and native tendon tissue (p < 0.01). Tenascin-C and scleraxis expressions were highest in MSC derived from cord blood compared to MSC derived from other sources, though both tenascin-C and scleraxis were expressed at significantly lower levels in all MSC compared to native tendon tissue (p < 0.01). Conclusions: These findings demonstrate that the MSC source impacts the cell properties relevant to tendon regeneration. Adipose derived MSC might be superior regarding their potential to positively influence tendon matrix reorganization. MSC Mesenchymale Stammzelle Sehne Fettgewebe Knochenmark Nabelschnur Kollagen Decorin Scleraxis Tenascin-C MSC Mesenchymal stromal cell Tendon Adipose tissue Bone marrow Umbilical cord Collagen Decorin Scleraxis Tenascin-C ddc:636 ddc:610
42	Hibernoma – two patients with a rare lipoid soft-tissue tumour Daubner, Dirk, Spieth, Stephanie, Pablik, Jessica, Paulus, Tobias, Laniado, Michael, Zöphel, Klaus 24 July 2015 (has links) Background: Hibernomas are rare benign soft-tissue tumours arising from brown fat tissue. Although imaging characteristics are not specific certain imaging features, common locations and patient demographics may suggest hibernoma as a differential diagnosis. Case presentation: We report on two 48-year-old male patients with hibernoma. The tumour presented with local swelling of the inguinal region in the first patient and was an incidental imaging finding in the second patient. Imaging included magnetic resonance imaging in both patients and computed tomography as well as 18 F-fluorodeoxyglucose positron emission tomography-computed tomography in the second patient. In both cases histological diagnosis was initially based on excisional and needle core biopsy, respectively. Complete surgical resection confirmed the diagnosis of hibernoma thereafter. Conclusion: In soft tissue tumours with fatty components hibernoma may be included into the differential diagnosis. Because of the risk of sampling errors in hibernoma-like tissue components of myxoid and well-differentiated liposarcoma, complete resection is mandatory. This article also reviews the current imaging literature of hibernomas. info:eu-repo/classification/ddc/610 ddc:610
43	Identification of pathways in liver repair potentially targeted by secretory proteins from human mesenchymal stem cells Winkler, Sandra, Hempel, Madlen, Brückner, Sandra, Tautenhahn, Hans-Michael, Kaufmann, Roland, Christ, Bruno January 2016 (has links) Background: The beneficial impact of mesenchymal stem cells (MSC) on both acute and chronic liver diseases has been confirmed, although the molecular mechanisms behind it remain elusive. We aim to identify factors secreted by undifferentiated and hepatocytic differentiated MSC in vitro in order to delineate liver repair pathways potentially targeted by MSC. Methods: Secreted factors were determined by protein arrays and related pathways identified by biomathematical analyses. Results: MSC from adipose tissue and bone marrow expressed a similar pattern of surface markers. After hepatocytic differentiation, CD54 (intercellular adhesion molecule 1, ICAM-1) increased and CD166 (activated leukocyte cell adhesion molecule, ALCAM) decreased. MSC secreted different factors before and after differentiation. These comprised cytokines involved in innate immunity and growth factors regulating liver regeneration. Pathway analysis revealed cytokine-cytokine receptor interactions, chemokine signalling pathways, the complement and coagulation cascades as well as the Januskinase-signal transducers and activators of transcription (JAK-STAT) and nucleotide-binding oligomerization domain-like receptor (NOD-like receptor) signalling pathways as relevant networks. Relationships to transforming growth factor beta(TGF-beta) and hypoxia-inducible factor 1-alpha (HIF1-alpha) signalling seemed also relevant. Conclusion: MSC secreted proteins, which differed depending on cell source and degree of differentiation. The factors might address inflammatory and growth factor pathways as well as chemo-attraction and innate immunity. Since these are prone to dysregulation in most liver diseases, MSC release hepatotropic factors, potentially supporting liver regeneration. info:eu-repo/classification/ddc/610 ddc:610
44	Der Einfluss endokriner Disruptoren auf das Fettgewebe der Sprague-Dawley-Ratte / The influence of endocrine disruptors on the fat tissue of the Sprague-Dawley-rat Müller, Matthias 03 July 2008 (has links) No description available. 610 Medizin, Gesundheit Medicine DPB BPA BP2 LIN PROC E2 Fettgewebe ED Insulin Glucose Leptin IGF-1 DPB BPA BP2 LIN PROC E2 fat tissue EDs insulin glucose leptin IGF-1 44.89 Endokrinologie/44.92 Gynäkologie
45	Gene expression of tendon markers in mesenchymal stromal cells derived from different sources Burk, Janina, Gittel, Claudia, Heller, Sandra, Pfeiffer, Bastian, Paebst, Felicitas, Ahrberg, Annette B., Brehm, Walter January 2014 (has links) Background: Multipotent mesenchymal stromal cells (MSC) can be recovered from a variety of tissues in the body. Yet, their functional properties were shown to vary depending on tissue origin. While MSC have emerged as a favoured cell type for tendon regenerative therapies, very little is known about the influence of the MSC source on their properties relevant to tendon regeneration. The aim of this study was to assess and compare the expression of tendon extracellular matrix proteins and tendon differentiation markers in MSC derived from different sources as well as in native tendon tissue. MSC isolated from equine bone marrow, adipose tissue, umbilical cord tissue, umbilical cord blood and tendon tissue were characterized and then subjected to mRNA analysis by real-time polymerase chain reaction. Results: MSC derived from adipose tissue displayed the highest expression of collagen 1A2, collagen 3A1 and decorin compared to MSC from all other sources and native tendon tissue (p < 0.01). Tenascin-C and scleraxis expressions were highest in MSC derived from cord blood compared to MSC derived from other sources, though both tenascin-C and scleraxis were expressed at significantly lower levels in all MSC compared to native tendon tissue (p < 0.01). Conclusions: These findings demonstrate that the MSC source impacts the cell properties relevant to tendon regeneration. Adipose derived MSC might be superior regarding their potential to positively influence tendon matrix reorganization. info:eu-repo/classification/ddc/636 ddc:636 info:eu-repo/classification/ddc/610 ddc:610

Page generated in 0.0341 seconds