Made available in DSpace on 2015-05-08T14:59:38Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 6363993 bytes, checksum: e8cfe3d37bd9a2f2218e5522ba2ddff7 (MD5) Previous issue date: 2009-08-11 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The analysis of properties of heat and mass transfer in foods has recently received increased attention due to its importance in modeling, and in the optimization of equipments and processes. In this work, an experimental procedure was performed with commercial-type, natural and skimmed yoghurt purchased from the local market, in order to estimate the thermal diffusivity. The experimental procedure was developed for liquid sample. The sample was placed between two metal plates. The thermal diffusivity was obtained from measurements of frontal temperature. The experimental procedure was performed in a micro-flash apparatus, model 457 LFA and thermal conductivity was determined in the calorimeter model 2920 mark TA Modulated. The results for thermal diffusivity and thermal conductivity present a good agreement with the available values from the literature. / A análise das propriedades de transferência de calor e massa em alimentos tem recebido maior atenção recentemente devida sua importância fundamental na modelagem, na otimização e nos projetos de equipamentos para processos. Neste trabalho, procedimentos experimentais foram realizados com iogurtes comerciais do tipo natural e desnatados, obtidos no mercado local com o objetivo de estimar a difusividade térmica. O procedimento experimental foi desenvolvido para líquidos entre placas metálicas e uma amostra liquida e mediu a avaliação da temperatura sobre a face frontal, a partir da qual foi obtida a difusividade térmica. O procedimento experimental foi realizado em um aparelho micro-flash, modelo LFA 457 da Netzsch e o calor específico foi determinados no calorímetro modelo 2920 Modulated marca TA. Os resultados obtidos para a difusividade térmica e condutividade térmica apresentam boa concordância com os valores disponíveis na literatura.

Made available in DSpace on 2014-12-17T15:01:26Z (GMT). No. of bitstreams: 1 LuzianyACF_DISSERT.pdf: 949661 bytes, checksum: 3ac9543ca6f7961fb8c420391d30326b (MD5) Previous issue date: 2011-07-08 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / In this work, a pneumatic dryer has been designed and assembled in laboratory scale in order to study and evaluate configurations more efficient for application in drying of important materials of Northeast region in Brazil. The equipment was tested with drying of corn and rice grains, in conditions of temperature and air velocity at 80 oC and 35 m/s, respectively. For this type of dryer, it is recommended to work at temperatures above 200 ?C and air velocity with higher dynamic pressure. However, even under operating conditions below what it is recommended, the results obtained with the pneumatic dryer were satisfactory. In addition, experiments of drying were performed by using a cabinet dryer (batch dryer) under the same conditions used in the pneumatic dryer. Flash one curves for the corn were fitted satisfactorily by applying of the Lewis model, while a better agreement was found for rice by using the Page model. The data obtained with both drying processes allowed to compare the performance between pneumatic and batch dryers. In respect to drying rate, the pneumatic dryer presented a similar performance to the batch dryer during processing with corn and a superior performance to the last one during processing with rice. Therefore, it was possible to verify that the pneumatic dryer assembled in this preliminar study can be applied for different materials and under different operating conditions / Este trabalho tem como objetivo principal ? concep??o, montagem e opera??o de um secador pneum?tico do tipo flash com o prop?sito de avaliar novas configura??es de secadores, apresentando uma import?ncia regional no contexto da produ??o agr?cola de gr?os. O equipamento operou com temperaturas na ordem de 80?C e velocidade do ar de secagem igual a 35 m/s. Para este tipo de secador recomenda-se temperaturas superiores a 200?C e velocidades com alta press?o din?mica. O equipamento operou com um bom desempenho na secagem do milho e arroz. Para efeito comparativo, tamb?m foi utilizado um secador de bandejas para secagem do milho e do arroz com casca em condi??es operacionais similares as praticadas com secador pneum?tico do tipo flash. Os resultados experimentais demonstraram que na secagem do milho ambos secadores responderam com desempenho an?logo no que ser refere ? taxa de secagem. J? na secagem do arroz com casca, o secador pneum?tico apresentou um desempenho superior ao secador de bandejas. Portanto, a partir dos dados experimentais as curvas para a secagem do milho foram melhor correlacionadas com o modelo de Lewis, enquanto que as curvas de secagem do arroz com casca foram melhor ajustadas com o modelo de Page. Assim, pode-se concluir que a configura??o montada apresenta aspectos promissores que podem ser estudados utilizando-se materiais com diferentes caracter?sticas e em diferentescondi??es operacionais

Organismos são capazes de diferenciar estímulos sensoriais gerados independentemente pelo ambiente dos estímulos causados por sua própria ação no mundo. Esse processo depende de mecanismos neurais e cognitivos que unam suas ações às percepções por elas geradas. Objetivo desse trabalho foi investigar a interação da ação com a percepção visual no domínio temporal. Em cinco experimentos psicofísicos, foi estudado o efeito modulatório da ação no efeito flash-lag e em tarefas envolvendo estimativas de intervalos temporais. Nossos resultados mostraram que o planejamento e/ou execução de atos motores voluntários são capazes de reduzir em algumas dezenas de milissegundos as latências com que estímulos visuais são percebidos e também de reduzir as estimativas de intervalos temporais. A redução dessas latências é maior quando a conseqüência sensorial da ação é apresentada na fóvea, assim como quando existe um atraso entre a ação e o estímulo por ela causado. / Organisms are able to distinguish between sensory stimuli from the environment and sensory stimuli they cause. This process depends on neural and cognitive mechanisms that link actions to perceptions generated by these actions. This work aimed to investigate the interaction of action and perception in the temporal domain. In five psychophysical experiments, we have assessed the modulation of action in the flash-lag effect and in tasks involving temporal interval estimations. Our results showed that the planning and execution of a voluntary motor action are capable of reducing both visual perceptual latencies and temporal interval estimations. This reduction increases when the sensory consequences of motor actions are presented on the fovea and when a delay is injected between the action and the stimuli caused by it.

Vi har gjort vårt kandidatarbete på en webbyrå i Stockholm som heter Boman Communication. Där har vi gjort en databasdriven webbplats i flash och asp. Webbplatsen är ett komplement till en kurs som heter EBM. Kursen EBM hålls av myndigheten SBU som i sin tur är en kund till Boman. Målgruppen för EBM och därmed också webbplatsens målgrupp är bland annat professorer och läkare inom medicin och vård. På kursen tar man upp riktlinjer för hur man botar olika sjukdomar eller skador. Webbplatsen innehåller information om och till kursen. Den är uppdelad i tre delar: Allmän, Före kursen och Efter kursen. Den allmänna delen är till för att väcka ett intresse för kursen hos besökaren. Denna del kommer alla åt. De två övriga delarna kräver inloggning och är bara till för dem som anmält sig på kursen. Inloggningen är datumstyrd vilket innebär att när kursen inte har varit ännu kommer man till delen före kursen och när kursen har varit kommer man till delen efter kursen. Före kursen hittar man information på webbplatsen som man bör ta del av för att förereda sig till kursen, man kan även finna information om föreläsarna och övriga kursdeltagare. Efter kursen är webbplatsen uppdaterad med en sida för dokumentation som föreläsarna har rekommenderat, här finns även en utvärdering som kursdeltagarna uppmanas att fylla i. Vi har framförallt använt oss av programmen Macromedia Flash MX 2004 och Macromedia Dreamweaver MX 2004 men även program som Adobe Photoshop CS, Adobe Illustrator CS. / Detta är en reflektionsdel till en digital medieproduktion.

This is the end report of my final exam at Medieteknik. It covers the planning, work process and review of a Flash based web application that I set out to do. The goal was to create a user friendly, interactive e-shop with embedded functionality for playing music, displaying rich media content and researching possible payment solutions, to enable buying music files online. The project involved creating the user interface, the programmatic functionality of a Flash client (an SWF file). Also, setting up an Apache HTTP Server and establishing a working “bridge” between the Flash client and the web server with Flash Remoting components and AMFPHP. Furthermore, a web interface for adding music files and data to the server was created, built in HTML, CSS, JavaScript, PHP and MySQL. The project consisted of researching and implementing the technologies and techniques involved by iteratively collecting information, reading, testing, programming and evaluating the end results. Also, object oriented design and programming principles was applied in the development process. The software development methodologies – Agile development and extreme programming was practiced. The purpose was to gain experience in developing large scale client/server web applications dealing with creative processes, project planning, project management and problem solving. And in the longer perspective make use of the experience and knowledge in future projects. / Jag hade en idé om att skapa en e-shop med fokus på användarvänlighet och interaktivitet. Detta ledde till en klient/server-applikation med funktioner för att lyssna på musik och söka efter- och ta del av musikinformation. Syftet var att ge användare möjlighet att köpa musik i form av nedladdningsbara MP3-filer. Applikationen är huvudsakligen indelad i tre sektioner, en meny överst; en musikspelare placerad på vänster sida; och en s.k. content-del, ett slags fönster där all text och bildbaserat innehåll presenteras. Gränssnittets utformning är rent och enkelt med tydliga knappar och menyer för enkel navigation. Musik kan förhandslyssnas, och spelas upp med hjälp av en inbyggd musikspelare. Tanken är att ljudet ska kunna spelas upp i realtid från servern, dvs. strömmas. Detta medför att data inte lagras hos klienten och syftet är alltså att användaren ska kunna förhandslyssna på den musik han eller hon är intresserad av att köpa, men till sämre ljudkvalitet än den köpta MP3-filen. En display visar artistens eller gruppens namn, låtnamn, spelad tid, och total tid. Vidare finns knappar för att spela upp, stoppa, pausa och spela nästa respektive föregående låt. Slutligen finns ett reglage för att kunna styra spelposition. Som ett led i att förenkla köp av musik kan en spellista fyllas med valda låtar. Låtarna i denna lista kan man sedan köpa i ett svep eller var för sig. Detta innebär att användaren slipper leta upp en låt i taget, och lyssna på dem vart efter varandra. Ett webbformulär byggdes för att kunna tillhandahålla uppladdning och lagring av musikfiler och musikinformation. Webbformuläret är byggt i teknikerna HTML, CSS, PHP och JavaScript. Den innehåller en dynamiskt genererad tabell för att underlätta insättning av låtar. Webbformuläret kan med fördel utnyttjas av godkända parter (skivbolag, artister…), för att ladda upp och lagra musikfiler på servern. Detta resulterar i en arbetsbesparande process eftersom arbetsbördan fördelas till extern part. All musikinformation, så som artistnamn, låtnamn, utgivningsdatum, etc. lagras och hämtas från en MySQL-databas. Musikfiler lagras och spelas upp från filsystemet i en Apache HTTP Server. Applikationen utgörs av flera skikt. Det innebär att den är uppdelad i flera separata delar. En del består av gränssnittet, en SWF-fil som användaren kommer åt genom sin webbläsare. Gränssnittet opererar gentemot data som ligger lagrat på en webbserver. Vidare finns speciell programkod på servern för att ta emot-, skicka respektive manipulera data. Två typer av datalager används för att möjliggöra kommunikation av data mellan Flashklient (gränssnitt) och server – Flash Remoting och AMFPHP. Flash Remoting är ett slags kommunikationsprotokoll som Flashklienten använder för att kommunicera med server. AMFPHP används för att ”tolka” Flashkod som sänds via HTTP till server. AMFPHP omvandlar denna till, för servern, läsbar server-sidekod – PHP, som i sin tur kan manipulera data på servern, t.ex. lagra data i databasen. När data skickas till Flashklienten, omvandlar AMFPHP data till, för Flashfilen, läsbar kod. En stor del av projektet har varit av undersökande natur. Målet var att erhålla tekniska kunskaper i Flashmiljön för att kunna utveckla interaktiva webbapplikationer. Kunskap har inhämtats via litteratur i bokform, det inbyggda hjälpbiblioteket i Adobe Flash 8 Professional mjukvara, samt från internet, bland en mängd olika forum och webbsidor. Utvecklingsarbetet har bestått av en kombination av rörlig utveckling och extrem programmering. / +46 (0)708 38 08 03 guild2000@hotmail.com

La protéine de nucléocapside (NC) du virus de l’immunodéficience humaine (VIH-1) joue un rôle majeur dans les différentes étapes du cycle viral du VIH-1 : soit comme domaine fonctionnel de la polyprotéine Gag (NC-Gag) dans les phases tardives du cycle viral, soit sous sa forme mature NCp7 dans les phases précoces. Afin de mieux comprendre le rôle de la forme mature dans le cycle viral, nous avons cherché de nouveaux partenaires cellulaires spécifiques de la NCp7 et identifié la protéine nucléolaire, hNoL12, impliquée dans la maturation des ARNs ribosomaux. L’interaction NCp7/hNoL12 a été confirmée par co-IP, FRET-FLIM et double hybride chez la levure et le domaine d’interaction a été localisé entre les a.a. 22 et 61 correspondant au domaine 5’-3’-exonucléase de hNoL12. Nous avons développé un test pour caractériser cette activité et montré qu’elle est spécifique des ARN simples brins. Enfin, l’extinction de l’expression de hNoL12 entraine une diminution significative de l’infection par un lentivecteur modèle des phases précoces de l’infection soulignant l’implication fonctionnelle de hNoL12 dans cette phase de l’infection. Dans un second projet, nous nous sommes intéressés au devenir de la NCp7 dans les cellules infectées, suite à la transcription inverse. Nous avons généré des vecteurs lentiviraux composés de protéines NCp7 fusionnées à une étiquette tétracysteine permettant son marquage spécifique avec le dérivé de la fluorescéine (FlAsH). Nous avons alors étudié, par microscopie confocale, la distribution intracellulaire de la NCp7 dans des conditions proches de l’infection. Nos résultats indiquent qu’une grande partie de la NCp7 se dissocie du PIC durant son transport dans le cytoplasme. Toutefois, la perte de la NCp7 est une étape tardive qui se déroule proche du noyau confirmant ainsi que la décapsidation a lieu à la membrane nucleaire juste avant l’entrée du complexe de préintegration dans le noyau. Le troisième projet a porté sur le développement d’antiviraux ciblant la NCp7. Nous avons travaillé sur la vectorisation et la caractérisation des propriétés antivirales en milieu cellulaire, d’un peptide sélectionné in vitro pour sa capacité à inhiber l’action chaperonne de la NCp7. L’activité antivirale du peptide vectorisé vis-à-vis d’une infection par un vecteur lentiviral basé sur le VIH-1 s’est révélée décevante. / The Human Immunodeficiency Virus-1 (HIV-1) nucleocapsid protein (NC) plays a major role in the different steps of theviral lifecycle under its two forms; either as a domain of the polyprotein Gag (NC-Gag) in the late phase or as a matureNCp7 protein in the early phase. In order to better understand the role of the mature form in the viral cycle, we searchedfor new NCp7 specific cellular partners and identified the nucleolar protein hNoL12 which is known to be involved inthe maturation of ribosomal RNAs. The NCp7/hNoL12 interaction was confirmed by co- IP, FRET-FLIM, and yeast twohybrid. The interaction domain was localized between a.a. 22 and 61 on hNoL12; which corresponds to its putative 5’-3’-exonuclease domain. We developed an assay to monitor this activity and found it to be specific of single strand RNA.Finally, the cellular knockdown of hNoL12 resulted in a significant decrease in the infection by a pseudovirus mimickingthe early phase of the infection, emphasizing the functional involvement of hNoL12 in this phase. In a second project, wewere interested in the fate of the viral incoming NCp7 in the infected cells, after reverse transcription. We thus generatedlentiviral vectors composed of NCp7 fused to a tetracysteine tag enabling its specific labeling with the fluoresceinderivative FlAsH. We then studied by confocal microscopy, the intracellular distribution of NCp7 containing viralparticles in conditions close to the infection. Our results showed that an important proportion of the NCp7 moleculesdissociates from the PIC during its transport in the cytoplasm. However, the loss of NCp7 is a late step of this processand seems to take place close to the nucleus suggesting that the dissociation of the capsid occurs at the nuclear membranejust before the nuclear entry of the PIC. The third project concerns the development of antiviral inhibitors targetingNCp7. We worked on the vectorization and the characterization of the antiviral properties of a peptide selected in vitrofor its ability to inhibit the NCp7 chaperone activity. The inhibitory activity of the vectorized peptide on infection ofHeLa cells by a HIV-1 based lentiviral vector was found deceiving.

This Diploma thesis deals with the analysis and proposal of the pilot BI solution for the company Svoboda & Partner CZ s.r.o., engaged in the automechanic expertise. Fundamental objectives of this work focus particularly on the above mentioned pilot BI solutions and partial objectives deal with an analysis of the company and its needs, arising from business strategy and market analysis applications available to support the visualization of transaction reporting. These objectives have been achieved by analyzing the company's business model in chapter seven, further analysis of needs and problems within the company in chapter eight. Before the analysis, the diploma thesis deals with a survey of available applications that offer functionality of dynamically generated graphs to aid visualization of transaction reporting in chapter six and finally a major analysis and preparation of pilot BI and transaction reporting for this business which you can find from chapter 9 to chapter eleven. Contribution of this work for the company will be a complete analysis and delivery of all necessary documents in order to decide whether the above BI solutions to the company implement or not. In the case of a positive decision of the company, it is expected that thanks to BI solutions the ways of looking at corporate data can be more expanded, more precise and more operational management of the company, achieving better performance, higher profits and more efficient decision-making based on better data for decision making.

Thermal and flash photolysis studies of ligand-substitution reactions of cis-(pip)(L)M(CO)_4 by L' (pip = piperidine; L, L' = CO, phosphines, phosphites; M = Cr, Mo) implicate square-pyramidal [(L)M(CO)_4], in which L occupies a coordination site in the equatorial plane, as the reactive species. In chlorobenzene (= CB) solvent, the predominant species formed after flash photolysis and a steady-state intermediate for the thermal reaction is cis—[(CB)(L)M(CO)_4], for which rates of CB-dissociation increase with increasing steric demands of coordinated L. Rates of CB-dissociation from trans-[(CB)(L)M(CO)_4] intermediates, formed after photolysis but not thermally, exhibit no observable dependence on the steric properties of the coordinated L.

The feasibility of the QD-Flash concept, its fast write and erase times, is demonstrated together with storage times of 4 days at room temperature. The storage time of holes in (InGa)Sb QDs embedded in a (AlGa)P matrix can be extended by growth modifications to 10 y. Tunneling structures were recently demonstrated to solve the trade-off conflict between storage time and erase time. A QD-NVSRAM is suggested to become the first commercial application.

T1 mapping of myocardial tissue is important for diagnostics of myocardial fibrosis. Cardiac magnetic resonance imaging of small animals is challenging due to high heart and respiratory rates. Pulse sequences for T1 mapping are proposed in this thesis based on inversion recovery FLASH and Intragate FLASH. The sequence IR FLASH was compared to the reference sequence RARE on a static phantom. Both sequences were applied for measuring the myocardium of a rat. For T1 quantification a software in Matlab was developed. Using this software, T1 maps of rat myocardium were calculated.