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Graduation date: 1953

Author also know as S M Loveday / Fresh bakery products have a very short shelf life, which limits the extent to which manufacturing can be centralised. Frozen doughs are relatively stable and can be manufactured in large volumes, distributed and baked on-demand at the point of sale or consumption. With appropriate formulation and processing a shelf life of several months can be achieved.Shelf life is limited by a decline in proofing rate after thawing, which is attributed to a) the dough losing its ability to retain gas and b) insufficient gas production, i.e. yeast activity. The loss of shelf life is accelerated by delays between mixing and freezing, which allow yeast cells the chance to ferment carbohydrates.This work examined the reasons for insufficient gas production after thawing frozen dough and the effect of pre-freezing fermentation on shelf life. Literature data on yeast metabolite dynamics in fermenting dough were incomplete. In particular there were few data on the accumulation of ethanol, a major fermentation end product which can be injurious to yeast.Doughs were prepared in a domestic breadmaker using compressed yeast from a local manufacturer and analysed for glucose, fructose, sucrose, maltose and ethanol. Gas production after thawing declined within 48 hours of frozen storage. This was accelerated by 30 or 90 minutes of fermentation at 30;C prior to freezing.Sucrose was rapidly hydrolysed and yeast consumed glucose in preference to fructose. Maltose was not consumed while other sugars remained. Ethanol, accumulated from consumption of glucose and fructose, was produced in approximately equal amounts to CO2, indicating that yeast cells metabolised reductively.Glucose uptake in fermenting dough followed simple hyperbolic kinetics and fructose uptake was competitively inhibited by glucose. Mathematical modelling indicated that diffusion of sugars and ethanol in dough occurred quickly enough to eliminate solute gradients brought about by yeast metabolism.

The search for the population s better quality of life is attracting the food industry interest in developing products with functional characteristics, offering wholefoods, fortified, enriched or improved food, causing effects potentially beneficial to healthy in the preventive and therapeutic aspects. This work aimed to elaborate a frozen yogurt with functional properties from yogurt supplemented with prebiotic (inulin), calcium caseinate and probiotics. The lactic cultures used were the traditional and the probiotic lactic cultures in three different concentrations (0,5%, 1,0% e 1,5%). The elaboration process of the frozen occurred from the development of the yogurt, in concentrations already mentioned, observing the pH values and, later, through the processes of homogenization of the ingredients, mixing in low temperature (ice cream maker), packing and freezing of the product at a temperature of -22ºC. The carried out physicochemical analyses were for pH, acidity expressed as lactic acid, lactose level, ash level, protein level, fat level and overrun; the microbiological analysis, in a 35-day period of storage and in 7-day intervals, to evaluate the feasibility of the traditional species lactic cultures Streptococcus salivarius ssp. thermophilus, Lactobacillus delbrueckii ssp. Bulgaricus and, probiotics Lactobacillus acidophilus e Bifidobacterium sp. Microbiological analyses of the traditional lactic cultures and liofilized probiotics were carried out for the certification of the number of microorganisms, respectively. Sensorial analysis was carried out in three alternate days, with two days in the UNIFRA (Franciscan University of Santa Maria, RS) Technical and Dietetic Laboratory and the third day in the UFSM (Federal University of Santa Maria, RS) Sensorial Analysis Laboratory with the analyzed features being: color, flavor, smell, consistency and total quality using a structured hedonic scale with 9 scores. The intention of buying the product and its preference were evaluated in this research making 105 non-trained tasters. The experimental data generated through the sensorial analysis results were submitted to variance analysis (ANOVA) and the differences of the means compared through the Tukey. Concentrations of 1.0% and 1.5% of traditional cultures and probiotic had no significant difference in relation to sensory analysis developed. To order the test was used in preference to the table and Newell MacFarlane where the formulation of 0.5% of crops and traditional probiotic milk obtained the lower acceptability. / A busca na melhoria da qualidade de vida da população vem despertando o interesse na indústria alimentícia em desenvolver produtos com características funcionais, proporcionando alimentos integrais, fortificados, enriquecidos ou melhorados, causando efeitos potencialmente benéficos à saúde nos aspectos preventivos e terapêuticos. Este trabalho teve como objetivo elaborar um frozen yogurt com propriedades funcionais a partir de iogurtes suplementados com prebiótico (inulina), caseinato de cálcio e probióticos. As culturas lácticas utilizadas foram as tradicionais e as culturas lácticas probióticas em três diferentes concentrações (0,5%, 1,0% e 1,5%). O frozen foi elaborado a partir do desenvolvimento do iogurte, nas concentrações já descritas, observando os valores de pH e, posteriormente, a realização dos processos de homogeneização dos ingredientes, batimento em baixa temperatura (sorveteira), envase e congelamento do produto a temperatura de -22ºC. As análises físico-químicas realizadas foram de pH, acidez expressa em ácido láctico, teor de lactose, teor de cinzas, teor de proteína, teor de gordura e overrun; a análise microbiológica, num período de 35 dias de armazenamento e em intervalos de sete dias, para avaliar a viabilidade das culturas lácticas das espécies tradicionais Streptococcus salivarius ssp. thermophilus, Lactobacillus delbrueckii ssp. bulgaricus e, probióticas Lactobacillus acidophilus e Bifidobacterium sp. Foi realizada a análise microbiológica das culturas lácteas tradicionais e probióticas liofilizadas para certificação do número de microrganismos respectivamente. A análise sensorial foi realizada em três dias alternados sendo analisados os atributos cor, sabor, aroma, consistência e qualidade global utilizando uma escala hedônica estruturada de nove pontos. Avaliou-se nesta pesquisa a intenção de compra do produto e sua preferência, totalizando 105 provadores não treinados. Os dados experimentais gerados pelos resultados da análise sensorial foram submetidos à análise de variância (ANOVA) e as diferenças das médias comparadas através do teste de Tukey. As concentrações de 1,0% e 1,5% de culturas tradicionais e probióticas não tiveram diferença significativa em relação à análise sensorial desenvolvida. Para o teste de ordenação de preferência foi utilizada a tabela de Newell e MacFarlane onde a formulação de 0,5% de culturas lácteas tradicionais e probióticas obteve a menor aceitabilidade.

The medium offering the greatest resistance to heat transfer from the freeze-drying shelf to the moving and subliming surface is the space between the flat shelf top and the concave vial bottom. The resistance to heat transfer can be greatly reduced by improving the thermal conductivity of the intervening space. Several heat transfer augmentation devices, including a multilayered corrugated aluminum quilt and a conformable fluid cushion device, which fill this gap are described. The devices are inexpensive and easy to use. Experimental data show that the resistance of the intervening space is reduced appreciably and the drying rate is greatly increased. The fluid cushion device is superior to the aluminum quilt as it reduces the consequences of spillage of solution and provides greater intervial uniformity among the same batch of vials. Drying times obtained in experiments with and without the fluid cushion device are compared here for different sizes and different types of vials. Product evaluation is conducted by measuring the reconstitution time and observing the product under a microscope. The solubilities of two univalent electrolytes, sodium chloride and potassium chloride, have been measured in eight cosolvent-water binary systems. The solubility of both the solutes has been found to be adequately described by the log-linear solubility equation, log S(m) = log S(w) + fσ. The rank order of the desolubilization slopes obtained for the electrolyte solutes is compared with the solubilization of nonelectrolyte solutes. These results indicate that a cosolvent which is most effective in solubilizing a nonelectrolyte is also most effective in desolubilizing an electrolyte. The solubility of oxacillin sodium in methanol-water mixtures has been determined at various temperatures ranging from +21 to -26 degrees centigrade. The data has been fitted to the log-linear relationship as proposed by Yalkowsky et. al. The heat of solution is determined using the van't Hoff equation and was found to be nearly constant at 1.2 Kcal/mole. There appears to be no dependency of the slope of the log S(m) vs. fraction cosolvent plot to the temperature. The data suggests that there is a polymorphic or amorphic transition of oxacillin at -14.5 degrees centigrade.

Heat treated orange juice, both pasteurized and concentrate, are being increasingly consumed in the U.S. Orange juice is primarily heat treated to increase its shelf life, by curbing the growth of microorganisms; and to inactivate pectin methylesterase, which demethylates pectin and leads to cloud loss in the juice. However, because of heat processing, orange juice undergoes undesirable flavor changes that decrease its acceptability to consumers. The objectives of this study were to differentiate between fresh frozen and heat treated orange juice employing descriptive analysis, and to determine by Osme, a gas chromatography-olfactometry (GCO) method, odor active volatiles that were either lacking or created in the heat treated juice. The second objective was to determine how changes in the odor-active volatile profile of heat treated orange juice, relates to changes in the aroma and flavor intensities of the samples as assessed by descriptive analysis. Through descriptive analysis, the panel was successful in significantly (p<0.05) separating the fresh, pasteurized, and concentrate samples. Orange, orange peel, sweet, and grassy descriptors were found to be important for fresh aroma and flavor, while cooked, yam, metallic, tamarind, green bean and artificial orange descriptors were higher in heat treated samples. Using Osme, it was possible to separate fresh frozen from heat treated orange juice, on the basis of their aroma profiles. Fresh frozen samples show a higher concentration of peaks tentatively identified as gamma-butyrolactone, citral, nonanal, carvone, perillaldehyde, carvyl propinate, valencene, and other unidentified peaks possessing descriptors such as floral, lime, citrus, pine, bamboo leaf, metallic, and vinyl. Pasteurized samples show a larger concentration of peaks tentatively identified as hexanol, octanol, nerol / carveol, myrcene, 2-octanone, p-cymene, terpenen-4-ol, betacitronellol, and other unidentified peaks with descriptors such as cilantro, vinyl, melon, mushroom, and metallic. Descriptors such as orange, orange peel, sweet, grapefruit, and grassy are more pronounced in the fresh samples and are similar to the odor descriptors of Osme peaks higher in the fresh samples. Descriptors such as cooked, artificial orange, yam, metallic, tamarind, and green bean are higher in the pasteurized samples, and are similar to the odor descriptors of peaks higher in these samples. / Graduation date: 1999

The effect of different blanching treatments and packaging materials on the enzymatic (lipoxygenase and peroxidase) activity and fatty acid stability of two different varieties of sweet corn on the cob (Jubilee and GH 2684) was evaluated during nine months of frozen storage at -23.3°C. The initial moisture content in the kernels of the two sweet corn varieties averaged 72.5%. After nine months of frozen storage the moisture content in the kernels of corn depended greatly on the packaging material used. The ears stored in Cryovac B and E bags showed the best moisture retention (72.2% final moisture content), followed by the polyethylene bags (71.4%) while the ears stored without packaging material showed severe dehydration (70.1%). The peroxidase and lipoxygenase activities were determined using spectrophotometric assays on a crude extract obtained from liquid nitrogen powdered corn. Both unblanched varieties of sweet corn showed similar initial peroxidase specific activity and general behavior during the nine months of frozen storage. The presence of lipoxygenase isozymes with different thermal stabilities in both varieties was suggested by the higher lipoxygenase specific activity found in Jubilee after freezing and nine months of frozen storage (0.135 units/mg protein) compared with the GH 2684 variety (0.115 units/mg protein). Complete inactivation of lipoxygenase was obtained after 9 minutes steam blanching at 100°C. Peroxidase was more heat resistant showing some remaining specific activity after 9 minutes steam blanching with a complete inactivation after 15 minutes steam blanching. No regeneration of either enzyme was observed during the nine months of frozen storage suggesting a permanent disruption of the active site of both enzymes. Relative fatty acid content was determined by gas chromatographic analysis of fatty acids methyl esters. The major fatty acids present in both varieties were palmitic (14.93%), stearic (2.79%), oleic (31.54%), linoleic (46.87%) and linolenic (1.89%) acids. Good stability of the polyunsaturated fatty acids was observed during the nine months storage at -23.3°C, with autoxidation as the main mechanism responsible for the decrease in the relative percent of polyunsaturated fatty acids. Some enzymatic oxidation also occurred, decreasing the linolenic acid content. The control of the degradation of polyunsaturated fatty acids depended mostly on the frozen storage temperature (-23.3°C) and not on the oxygen permeability of the different packaging materials. The results obtained in our study suggested that blanching of the ears of sweet corn had an important effect on reducing the enzyme activity but little effect on the polyunsaturated fatty acid degradation after 9 months of storage at -23.3°C. / Graduation date: 1994

Frozen soil has a major effect in many hydrologic processes, and its impacts are difficult to predict. A prime example is flood forecasting during spring snowmelt within the Canadian Prairies. One key driver for the extent of flooding is the antecedent soil moisture and the possibility for water to infiltrate into (partly) frozen soils. Therefore, these situations are crucial for accurate flood prediction at every spring. The main objective of this study was to evaluate the water flow and heat transport within available hydrological models to predict the impact of frozen and partly frozen soil on infiltration and percolation. A standardized data set was developed for water flow and heat transport into (partial) frozen soil by laboratory experiments using fine sand within a one-dimensional (1-D) soil column. A 1-D soil column having a length of 107 cm and diameter of 35.6 cm was built and equipped with insulation to limit heat exchange only through the soil surface. A data logger collected the moisture content and temperature by five FDR sensors which have been installed at a distance of 15 cm from each other. During the experiments, temperature, soil moisture, and percolated water was observed at different freezing conditions (-5°C, -10°C, and -15°C) as well as at thawing conditions when the air temperature was increased to +5°C. Distribution of soil moisture and soil temperature in the soil column was plotted for the experimental data over the freezing and thawing period. As some of the water in the soil begins to freeze, a decrease in water content was observed with a sudden increase in soil temperature near 0°C or slightly below of 0°C. This was, in fact, only a decrease in unfrozen water, not a decrease in total water content and was caused by the latent heat during freezing. Soil temperature showed noticeable differences at the top and the bottom of soil column during the change of state of water. The heat flux at the lower soil column was strongly limited due to iii the overlying soil. Thus, the soil temperature at the lowest sensors stayed in a freezing condition over several days and was not changing the temperature due to the latent heat which was released during the freezing process. Significant variation in soil moisture content was found between the top and the bottom of the soil column at the starting of the thawing period. However, with increasing temperature, the lower depth of the soil column showed higher moisture content as the soil was enriched with moisture with higher transmission rate due to the release of heat by soil particles during the thawing cycle. The soil system did not remain in the isothermal state during the thawing cycle. Although gravitational gradient was mainly responsible for the infiltration rate into the partially frozen soil, the distribution of moisture was greatly influenced by the temperature gradient. Vadose zone modeling using HYDRUS-1D was applied to the data set. Numerical results of the modeling were calibrated using the experimental results. It showed that the newly developed benchmark data set were useful for the validation of numerical models. The use of such a validated freezing and thawing module implemented into larger scale hydrologic models will directly reduce the prediction uncertainty during flood forecasting. Moreover, these benchmark data sets will be useful for the validation of numerical models and for developing scientific knowledge to suggest potential code variations or new code development in numerical models. / February 2017

Master of Science / Department of Grain Science and Industry / J.M. Faubion / The market for frozen goods is expanding and the frozen dough goods sector still has potential to expand its market. It is well known that deterioration in bread quality occurs during frozen dough/bread production. In addition, it is known that dough rheology influences bread quality. To prevent deterioration of bread quality, many additives have been used and researched. Combinations of oxidants (potassium bromate and ascorbic acid) are widely used worldwide. However, potassium bromate may be carcinogenic to humans, and it has been detected in bread after baking. Since it has been prohibited or strictly limited in many countries, many researchers have tried to find a replacement. Ascorbic acid is safe for human intake, and does not persist in bread. However, it is not as effective as potassium bromate. Possible replacements in frozen doughs include oxidant (ascorbic acid)-enzyme combinations. This study evaluated the effects of ascorbic acid-specific enzyme combinations as a replacement for the potassium bromate in frozen dough and related the effects to dough behavior (gluten network strength) as evaluated by dynamic oscillation rheometry. Bread quality was evaluated by test baking. Based on the results from fresh baking studies, potassium bromate can be replaced by an optimum level combination of ascorbic acid and hemicellulase/endo-xylanase. This combination clearly improved loaf volume, and crumb grain over both control and potassium bromate containing doughs. For frozen dough/bread production, the addition of all additives improved bread quality, but ascorbic acid and endo-xylanase containing dough resulted in higher volume, and better crumb structure than did dough containing potassium bromate. Dough rheology experiments show that rheology was affected by both the process and additives. Strain sweeps gave the information about dough stability. Both the additives and proofing improved dough stability. Dough behavior (gluten network strength) was assessed by frequency sweeps. Dough containing ascorbic acid and endoxylanase was most stable during frozen dough processing.

Call number: LD2668 .T4 1986 H864 / Master of Science / Grain Science and Industry