The objective of this study is to provide a better understanding of suffusion characteristics of glacial soils and to present a simple yet reliable assessment procedure for determination of suffusion in the laboratory.Internal erosion by suffusion occurs in the core of an embankment dam when the ability of the soil to resist seepage forces is exceeded and voids are large enough to allow the transport of fine particles through the pores. Soils susceptible to suffusion are described as internally unstable. dams with core of broadly graded glacial moraines (tills) exhibit signs of internal erosion to a larger extent than dams constructed with other types of materials.The Suffusion behavior of glacial soils has been investigated through two different permeameter suffusion test have been employed, small scale permeameter and big scale permeameter. Details of the equipment along with its calibration, testing and sampling procedures are provided.The testing program were performed 9 test with different compaction degree in small scale permeameter and 2 test in big permeameter on internally stable categories of till soil. The categories are defined based on the soil grain size distribution and according to the methods developed by Kenney & Lau and Burenkova.Layers are identified with suffusion if the post-test gradation curve exhibit changes in distribution compared to the initial condition and also the tests revealed that the effect of grain size distribution and relative degree of compaction on the internal erosion susceptibility of glacial till soils at different hydraulic gradients

Tropical forests of the world are fast disappearing and there is a race to understand patterns of species distribution in space and time. Studying species distributions can provide better frameworks for conservation of these ecologically important patches of floral and faunal diversity. The island of Sri Lanka is a well known harbour of unique and highly threatened biodiversity. Tropical lowland forest is remnant in the south-west of the island now mainly existing in small patches. While most are small disturbed fragments, Sinharaja Forest Reserve represents one of the largest remaining patches of this important ecosystem. As a UNESCO World Heritage Site and a Man and Biosphere Reserve, it has a dual role as a conservation area and a historically important resource forest. While the distribution of vegetation diversity has been well documented, analyses of invertebrate species distributions are lacking. This thesis investigated a key arthropod group, ground dwelling ants, in relation to environmental gradients within the forest. Cumulative results demonstrate the high diversity of the forest patch. In an area representing less than half the reserve, over 173 ground dwelling ant species were found in distinct assemblages throughout the forest. Since the forest is located upon a series of parallel ridges, ant species distribution was first analysed in terms of this small elevation change. Species richness declined over a vertical incline from 430 m to 660 m, highlighting a possible small-scale, mountain mass effect. This section of the reserve is also characterised by a patch of once-logged forest (30 years previously). A study was undertaken to investigate whether there were residual effects of selective logging on the reserve. / Significant differences between species assemblages in once-logged forest and unlogged forest add to growing evidence that selectively logged forests continue to remain distinct from unlogged forest even after decades of regeneration. Ant distribution was then analysed for their relationship with habitat heterogeneity and tree species distribution. Long-term research on tree species in the SFR has demonstrated a close relationship to habitat complexity. Ant species appear to respond more to the structural heterogeneity of the vegetation than to actual topographic variation within the forest. From a conservation perspective, maintaining the integrity of this highly diverse forest is imperative. The impact of anthropogenic land uses surrounding the forest was investigated in terms of ant assemblages along the forest edges. Significant differences were found between assemblages within the edges bordered by different matrix types. Even relatively large forest remnants can be affected by the surrounding matrix land uses and encouraging the growth of structurally similar vegetation and maintaining low disturbance along the borders should attenuate the effect of the edge. Overall, the highly heterogeneous distribution of ant assemblages within the SFR demonstrates the potential for other small patches to be harbours of further species diversity. Future research should be undertaken to assess the diversity and distribution of ant species within this region and encourage the protection of this remnant diversity.

Le dimensionnement par optimisation est aujourd'hui d'un intérêt majeur, car il fournit un moyen fiable et rapide en vue de déterminer les performances souhaitées de dispositifs, tout en minimisant une fonction de coût. Nous sommes particulièrement intéressés par l'optimisation sous contraintes basée sur le calcul de gradients. Ces algorithmes nécessitent des valeurs précises des dérivées de la fonction objectif et des performances à contraindre. Evaluer ces dérivées exactes se révèle comme une tâche complexe et très laborieuse, vu que les fonctions de performances et de coûts sont souvent évaluées à partir d'algorithmes numériques complexes. La Dérivation Automatique est une technique efficace pour calculer les dérivées des fonctions décrites au moyen de programmes informatiques dans des langages de programmation de haut niveau tel que Fortran, C ou C + +. Cette technique s'utilise parfaitement pour l'optimisation avec des algorithmes basés sur le calcul de gradients, étant donné que les dérivées sont évaluées sans aucune erreur de troncature ou d'annulation. Ce travail emploie la Dérivation Automatique pour calculer les gradients de programmes de calcul des modèles de dispositifs électromagnétiques.

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L'objectif de cette étude, réalisée en collaboration avec l'Agence d'Essais Ferroviaire (AEF) de la SNCF, est de proposer une méthode de calcul de durée de vie sous chargements complexes (chargements multiaxiaux d'amplitude variable) en fatigue à grande durée de vie. La première partie du document présente une analyse critique de différentes méthodes de la littérature dans ce domaine. La seconde partie du document présente la base de données expérimentales établie en vue de l'identification et de l'évaluation de la méthode proposée. Cette base de données est constituée d'essais sur éprouvettes lisses sous une large gamme de sollicitations à complexité croissante (les essais vont de la traction d'amplitude constante à des essais de flexion-torsion hors phase d'amplitude variable) mais également sur roue de TGV à échelle 1 (essais réalisés à l'AEF, sous chargements d'amplitude variable inspirés d'enregistrements en service). La suite du document présente une nouvelle méthode de calcul de durée de vie sous chargements multiaxiaux d'amplitude variable. En conservant une base énergétique et la notion de seuil de "non endommagement" définies au cours des précédents travaux menés au LAMEFIP, cette proposition prend en compte l'effet, sur la durée de vie, de la répartition spatiale des contraintes dans la pièce. Basée sur une définition incrémentale du travail de déformation fourni au matériau, cette proposition s'affranchit de toute méthode de comptage de cycle et est apte à prévoir la durée de vie d'une structure soumise à une sollicitation quelconque d'amplitude variable. Les prévisions de cette méthode sont confrontées à des résultats de différents essais de la littérature, notamment obtenus sur des éprouvettes entaillées, et aux résultats expérimentaux de l'étude. Enfin la méthode proposée est appliquée à un cas industriel : la prévision de la tenue en fatigue de la toile d'une roue de train à grande vitesse sous chargements d'amplitude constante ou par blocs. Les prévisions de la méthode, appliquée en post-processeur des résultats de calculs éléments finis sur la roue, sont en bon accord avec les résultats d'essais.

This paper demonstrates that it is possible to generate a reasonably accurate coordinate system on randomly distributed processors, using only local information and local communication. By coordinate systems we imply that each element assigns itself a logical coordinate that maps to its global physical location, starting with no apriori knowledge of position or orientation. The algorithm presented is inspired by biological systems that use chemical gradients to determine the position of cells. Extensive analysis and simulation results are presented. Two key results are: there is a critical minimum average neighborhood size of 15 for good accuracy and there is a fundamental limit on the resolution of any coordinate system determined strictly from local communication. We also demonstrate that using this algorithm, random distributions of processors produce significantly better accuracy than regular processor grids - such as those used by cellular automata. This has implications for discrete models of biology as well as for building smart sensor arrays.

myotubesProper muscle function depends upon the fine tuning of the different molecular components of the neuromuscular junction (NMJ). Synaptic acetylcholinesterase (AChE) is responsible for rapidly terminating neurotransmission. Neuroscientists in the field have elucidated many aspects of synaptic AChE structure, function, and localization during the last 75 years. Nevertheless, how the enzyme is regulated and targeted to the NMJ is not completely understood. In skeletal muscle the synaptic AChE form derives from two separate genes encoding the catalytic and the collagenic tail (ColQ) subunits respectively. ColQ-AChE expression is regulated by muscle activity; however, how this regulation takes place remains poorly understood. We found that over or down-regulation of ColQ is sufficient to change the levels of AChE activity by promoting assembly of higher order oligomeric forms including the collagen-tailed forms. Furthermore, when peptides containing the Proline Rich Attachment Domain (PRAD), the region of ColQ that interacts with the AChE, are fed to muscle cells or cell lines expressing AChE, they are taken up by the cells and retrogradely transported to the endoplasmic reticulum (ER)/Golgi network where they induce assembly of newly synthesize AChE into tetramers. This results in an increase, as a consequence, in total cell associated AChE activity and active tetramer secretion, making synthetic PRAD peptides potential candidates for the treatment of organophosphate pesticides and nerve gas poisoning. To study the developmental regulation of ColQ-AChE we determined the levels of ColQ and ColQ mRNA in primary quail muscle cells in culture and as a function of muscle activity. Surprisingly, we found dissociation between transcription and translation of ColQ from its assembly into ColQ-AChE indicating the importance of posttranslational controls in the regulation of AChE folding and assembly. Furthermore, we found that the vast majority of the ColQ molecules in QMCs are not assembled into ColQ-AChE, suggesting that they can have alternative function(s). Finally, we found that the levels of ER molecular chaperones calnexin, calreticulin, and particularly protein disulfide isomerase are regulated by muscle activity and they correlate with the levels of ColQ-AChE. More importantly, our results suggest that newly synthesized proteins compete for chaperone assistance during the folding process.

Understanding what drives variation in species diversity in space and time and limits coexistence in local communities is a main focus of community ecology and biogeography. My doctoral work aims to document patterns of ant diversity and explore the possible ecological mechanisms leading to these patterns. Elucidating the processes by which communities assemble and species coexist might help explain spatial variation in species diversity. Using a combination of manipulative experiments, broad-scale surveys, behavioral assays and phylogenetic analyses, I examine which ecological processes account for the number of species coexisting in ant communities. Ants are found in most terrestrial habitats, where they are abundant, diverse and easy to sample (Agosti et al. 2000). Hölldobler and Wilson (1990) noted that competition was the hallmark of ant ecology, and we know that ant diversity varies along environmental gradients (Kusnezov 1957). Thus ants are an ideal taxon to examine the factors shaping the structure of ecological communities and how the determinants of community structure vary in space.

Nyanza Gulf is a large, shallow and long river-influenced embayment located in northeastern Lake Victoria. The gulf opens to the main lake through the narrow and deep Rusinga Channel, the exchange zone between the two ecosystems with different physical chemical and biogeochemical conditions. The main goals of this study are to characterize physicochemical and nutrient gradients along the gulf-main-lake transect, characterize and quantify the water and nutrient fluxes between the gulf and the main lake, and assess the response of phytoplankton community and photosynthesis to the spatially varying physical and nutrient conditions along the study transect. Between March 2005 and March 2006, measurements of physicochemical profiles as well as nutrient and the phytoplankton community analysis were conductued monthly along the study transect. Additionally, analysis of different surficial sediment phosphorus fractions was done in order to asses the potential role of bottom sediment in contributing to phosphorus enrichment in the lake water column. A box mass balance model was used to calculate the exchange of water and nutrient fluxes between different zones along the study transect and to estimate ecosystem metabolism in the gulf and the channel. Spatial variability in physicochemical and biogeochemical conditions was observed along the study transect, especially between the shallow and river-influenced inner-gulf, the deep and physically active Rusinga Channel, and the main lake, mainly in response to river inputs and varying morphometry along the study transect. The gulf had significantly higher electrical conductivity (EC), turbidity, total nitrogen (TN), and dissolved reactive silica (DRSi) but the levels declined monotonically along the channel in response to mixing with the main lake water. The channel and the main lake had, respectively, significantly higher dissolved inorganic nitrogen (DIN) and soluble reactive phosphorus (SRP) compared to the gulf. Spatial variability in morphometry and exposure to varying wind forcing lead to differential mixing and differential heating and cooling along the transect, resulting in density driven fronts and horizontal exchange of water and nutrients between the gulf and the main lake. Upwelling and downwelling maintained mixing conditions in the channel which consequently influenced nutrient recycling, the light environment and hence affecting phytoplankton community composition and productivity. The net residual water flow from the gulf to the main lake was 36 m3/s but the mixing flux was approximately 20 times higher and both fluxes accounted for a gulf exchange time of 1981 days. The advective and mixing fluxes between the gulf and the main lake resulted in net export of dissolved inorganic phosphorus (DIP; 400 kg P/d) from the main lake into the gulf and net export of DRSi (10 t Si/d) from the gulf into the main lake. In the deep, narrow and physically active Rusinga Channel there was net production of dissolved nutrients whereas in the gulf there was net consumption of dissolved nutrients, which helped to maintain high net ecosystem production (NEP; 566 mg C/m2/d) in the gulf in contrast the channel which showed net heterotrophy. The high NEP in the gulf and the associated high nutrient demand coupled with possibly low SRP to DIN supply ratio lead to P limitation of algal growth in the gulf as indicated by all indicators of nutrient status. This has important implications for management since increased P input into the gulf will translate into increased algal blooms in the gulf and therefore compromise water quality. Spatial variability in physical conditions and nutrient status along the study transect influenced phytoplankton community composition and photosynthesis. The shallow and turbid gulf was dominated by cyanobacteria but diatoms dominated in the channel in response to reduced turbidity and increased physical mixing and nutrient availability (DRSi, SRP). In the main lake seasonal stratification and deep mixing depth favoured both cyanobacteria and diatoms. The phytoplankton community in channel had a higher photosynthetic capacity (Fv/Fm, PBm) compared to both the gulf and the main lake.