Made available in DSpace on 2014-06-11T19:28:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-11-29Bitstream added on 2014-06-13T18:32:29Z : No. of bitstreams: 1 messora_mr_me_araca.pdf: 1191442 bytes, checksum: 61d434d8954fce1c10d27e08d6201d16 (MD5) / Universidade Estadual Paulista (UNESP) / Objetivo: O propósito deste estudo foi avaliar, histologicamente, a influência do Plasma Rico em Plaquetas (PRP) no reparo ósseo de defeitos de tamanho crítico (DTC) criados cirurgicamente em calvárias de ratos. Material e Método: 32 ratos foram divididos em 2 grupos: C (controle) e PRP (Plasma Rico em Plaquetas). Um DTC de 8 mm de diâmetro foi criado na calvária de cada animal. No Grupo C, o defeito foi preenchido somente com coágulo sangüíneo. No Grupo PRP, o defeito foi preenchido com Plasma Rico em Plaquetas. Cada grupo foi subdividido para eutanásia em 4 ou 12 semanas pós-operatórias (n=8). Foram realizadas análises histológica e histométrica. A quantidade de osso neoformado foi calculada como uma proporção da área total do defeito original. Esses valores foram transformados em arcoseno para a análise estatística (ANOVA, Tukey, p < 0.05). Resultados: Nenhum defeito reparou completamente com tecido ósseo. O Grupo PRP apresentou significativamente mais neoformação óssea que o Grupo C, tanto em 4 semanas (17,68% e 7,20%, respectivamente) como em 12 semanas (24,69% e 11,65%, respectivamente) pós-operatórias. Conclusão: Dentro dos limites deste estudo, pode-se concluir que o PRP aumentou significativamente o reparo ósseo de defeitos de tamanho crítico em calvárias de ratos. / The purpose of this study was to histologically analyze the influence of Platelet Rich Plasma (PRP) on bone healing in surgically created critical-size-defects (CSD) in rat calvaria. 32 rats were divided into 2 groups: C (control) and PRP (Platelet Rich Plasma). An 8 mm diameter CSD was created in the calvarium of each animal. In Group C the defect was filled by blood clot only. In Group PRP it was filled with Platelet Rich Plasma. Both groups were divided into subgroups (n=8) and euthanized at either 4 or 12 weeks post-operative. Histometric, using image analysis software, and histologic analyses were performed. Amount of new bone was calculated as percentage of total area of original defect. Percentage data were transformed into arccosine for statistical analysis (ANOVA, Tukey, p < 0.05). No defect completely regenerated with bone. Group PRP had a statistically greater amount of bone formation than Group C at both 4 (17.68% and 7.20%, respectively) and 12 weeks (24.69% and 11.65%, respectively) post-operative. Within the limits of this study, it can be concluded that PRP significantly enhanced bone healing in critical-size-defects in rat calvaria.

Orientador: Enilson Antonio Sallum / Dissertaçao (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-08T13:02:16Z (GMT). No. of bitstreams: 1 Suaid_FabriciaFerreira_M.pdf: 1294405 bytes, checksum: 8106398e3a230d0f3fbccbd2dd04a6cd (MD5) Previous issue date: 2007 / Resumo: O objetivo do presente trabalho foi avaliar histometricamente, o processo de cura dos defeitos periodontais tipo retrações gengivais, criados cirurgicamente em cães, após serem tratados pela técnica do enxerto de tecido conjuntivo subepitelial em associação com o plasma rico em plaquetas (PRP). Inicialmente foram criados cirurgicamente defeitos de retração Classe I de Miller nos caninos superiores de seis cães de raça indefinida. Após um mês de cronificação, os defeitos bilaterais semelhantes foram aleatoriamente designados a receber os seguintes tratamentos: Lado 1: enxerto de tecido conjuntivo subepitelial associado ao uso de PRP; Lado 2: enxerto de tecido conjuntivo subepitelial. Decorridos 45 dias do tratamento os animais foram sacrificados e foram obtidas as peças para análise histológica dos seguintes parâmetros histométricos: novo cemento formado com fibras inseridas, novo osso, extensão do epitélio sulcular e juncional, área de adaptação conjuntiva e extensão do defeito. Observou-se uma maior extensão linear de novo cemento estatisticamente significante (p⿤ 0,05) nos dentes tratados com o PRP (2,18 ± 0,78 mm) quando comparados aos dentes do lado controle (1,19 ± 0,62 mm). Todos os outros parâmetros não tiveram diferenças estatísticas. As médias obtidas nos lados teste e controle, respectivamente, foram: extensão de epitélio sulcular e juncional, 2,04 ± 0,57mm e 2,49 ± 0,82mm; adaptação conjuntiva 0,29 ± 0,28mm e 0,23 ± 0,18mm; novo osso â¿¿ 0,57 ± 0,95mm e â¿¿ 0,46 ± 1,34mm, e extensão do defeito 4,13 ± 0,83mm e 4,47 ± 0,58mm. Considerando os limites deste estudo, pode-se concluir que a associação do PRP ao enxerto de tecido conjuntivo, no tratamento de defeitos de retração, promoveu maior neoformação cementária quando comparado ao tratamento controle / Abstract: The aim of this study was to histometrically evaluate the healing process of gingival recessions treated by PRP in combination with subepithelial connective tissue graft (SCTG) and to compare it to that obtained with SCTG alone (Control). Six mongrel dogs were used in the experiment. Gingival recessions (5x7mm) were surgically created and exposed to plaque accumulation for 1 month. Contralateral defects were then randomly assigned to test group or control. Dogs were sacrificed 45 days after healing, and the blocks containing the experimental specimens were processed for histological analysis. The histometric parameters evaluated were: length of sulcular and junctional epithelium, connective tissue adaptation, new cementum, new bone and defect extension. A superior length of new cementum, statistically significant, was observed in sites treated with PRP (2.18 ± 0.78mm) in comparison with the control (1.19 ± 0.62mm). No statistical differences in any other parameters evaluated were detected. The extension of the sulcular and junctional epithelium was 2.04 ± 0.57 mm for the PRP group and 2.49 ± 0.82mm for the control. The new connective tissue adjacent to the root without cementum formation was 0.29 ± 0.28 mm and 0.23 ± 0.18 mm for the PRP group and control, respectively. Bone formation was â¿¿ 0.57 ± 0.95 mm for the PRP group and â¿¿ 0.46 ± 1.34mm for the control. Within the limits of this study, it was concluded that PRP in combination with subepithelial connective tissue graft, when compared to the other treatment (control), seems to be more effective in promoting new cementum formation / Mestrado / Periodontia / Mestre em Clínica Odontológica

The growth and yield response of strawberry (Fragaria X annassa Duch.) and cranberry (Vaccinium macrocarpon Ait) to various growth inhibitors, but especially to paclobutrazol (PP333), and fertilizers were investigated in glasshouse and field studies. PP333 decreased vegetative growth in a rate-dependent manner in both strawberry and cranberry. PP333 delayed fruit ripening in strawberry and decreased fruit set and fruit size in cranberry. PP333 decreased pollen germination of strawberry. Soil residues of PP333 were biologically active when measured 11 weeks after application in the strawberry study and when measured after 50 weeks in a cranberry study. In a field study with cranberry, PP333 caused a decrease in vegetative growth and an increase in flowering in the next season after treatment. Two-dimensional partitioning was used to account for yield variation from treatment effects on adjusted yield variates. The truss number was the most important contributor to yield variation in the strawberry, but was not affected by treatments. PP333 made a substantial contribution to yield variation in strawberry through its effect on fruit development and ripening. In the cranberry, fruit set was the major contributor to yield variation and PP333 influenced yield substantially through its effect on fruit set. In glasshouse studies, PP333 decreased shoot elongation in cranberry within 3 weeks of application, and increased the number of branches on primary shoots. Buds were formed within 7 weeks of treatment and some of the buds contained flowers. Shoot growth was greater with a high rate of NPK fertilizer than with the low fertilizer rate and was greater in peaty soil than in sandy soil. While bud set was not modified by soil type or fertilizer rate, high NPK fertilizer decreased floral induction. Some effects of PP333 treatment were decreased by gibberellic acid, but generally these effects here not reversed. In a field study with the cranberry, PP333 increased flower bud set for flowering and non-flowering uprights but slightly decreased floral induction for non-flowering uprights. / Land and Food Systems, Faculty of / Graduate

INTRODUÇÃO: O plasma rico em plaquetas (PRP) é utilizado em medicina esportiva para aumentar e acelerar o processo de reparação tecidual em lesões tendineas e ligamentares, no intuito de proporcionar um retorno mais rápido às atividades esportivas. No entanto faltam estudos com alto nível de evidência comprovando sua eficácia no tratamento destas lesões. Com o objetivo de estudar o efeito da aplicação do PRP no sitio doador de enxerto do ligamento da patela para reconstrução do ligamento cruzado anterior (LCA), realizamos ressonância magnética aos seis meses de pós-operatório para avaliar o ligamento da patela. Também aplicamos questionários de função do joelho e realizamos teste isocinético após seis meses da cirurgia. MÉTODOS: O presente estudo prospectivo, randomizado, avaliador cego incluiu 27 pacientes. Comparamos doze pacientes em que foi utilizado o PRP no defeito criado no terço central do ligamento da patela para retirada de enxerto com quinze pacientes do grupo controle. Aos seis meses de cirurgia realizamos ressonância magnética, em que foi avaliada a área não regenerada do defeito no terço central do ligamento da patela, a área de secção transversa do ligamento e a altura da patela pelo índice de Insall- Salvati. Realizamos teste isocinético e aplicamos questionários específicos de função do joelho. Quantificamos a dor pós-operatória imediata com escala visual analógica (EVA) de dor. RESULTADOS: Aos seis meses de pós-operatório a área não regenerada do defeito no terço central do ligamento da patela foi 4,95 mm2 no grupo PRP e 9,38 mm2 no grupo controle (p=0,046, teste t de Student). A área de secção transversa do ligamento da patela no grupo PRP foi de 173,05 mm2 e no grupo controle 176,29 mm2 (p=0,856). O índice de Insall-Salvati nos grupos PRP e controle foi de 1,04 e 1,06, respectivamente (p=0,808). Não houve diferença entre os grupos nos resultados dos questionários e teste isocinético. Nos pacientes em que foi utilizado o PRP houve menos dor pós-operatória imediata (p=0,02). CONCLUSÃO: Concluímos, nessa amostra, que o uso do PRP no defeito do terço central do ligamento da patela na reconstrução do LCA, nos primeiros seis meses; determinou maior regeneração do defeito; diminuiu a intensidade da dor pós-operatória imediata; não ocasionou alterações estruturais na avaliação por ressonância magnética; e não modificou os resultados clínicos e funcionais da cirurgia / INTRODUCTION: Platelet-rich plasma (PRP) has been used in sports medicine to improve and accelerate ligaments and tendons healing, in an effort to provide a faster return to sports activities. However there is a lack of high evidence level studies to support its use. With the purpose to study the effect of PRP, patellar ligament was evaluated six months after harvesting its central third with magnetic resonance imaging (MRI). We also applied specific questionnaires of knee function and isokinetic testing. METHODS: This blinded, randomized, prospective study included 27 patients. Twelve patients in whom we added PRP in the patellar ligament after harvesting its central third for ACL reconstruction were compared to fifteen patients in the control group. After six months we evaluated the non-regenerated area of the patellar ligament, the cross section area of the patellar ligament and its length (Insall-Salvati index) with MRI. We also evaluated the results of isokinetic testing and specific questionnaires of knee function. Immediate post-operative pain was evaluated with visual analogical scale (VAS) score. RESULTS: The non-regenerated area of the patellar ligament was 4,95 mm2 in the PRP group and 9,35 mm2 in the control group (p=0,046 test). The cross section area of the patellar ligament in the PRP group was 173,05 mm2 and in the control group 176,29 mm2 (p=0,856). The Insall- Salvati index in the PRP and control group was 1,04 and 1,06, respectively (p=0,806). There was no difference between the groups in the questionnaires and isokinetic testing results. PRP group patients had less post-operative pain (p=0,02). CONCLUSION: We concluded, in this sample, that the use of PRP in the patellar ligament after harvesting its central third for ACL reconstruction, in the first six months: improved the ligament regeneration; reduced the intensity of immediate post-operative pain; did not cause structural changes in the patellar ligament in MRI; and did not modify the clinical and functional results of the procedure

INTRODUÇÃO: O plasma rico em plaquetas (PRP) é utilizado em medicina esportiva para aumentar e acelerar o processo de reparação tecidual em lesões tendineas e ligamentares, no intuito de proporcionar um retorno mais rápido às atividades esportivas. No entanto faltam estudos com alto nível de evidência comprovando sua eficácia no tratamento destas lesões. Com o objetivo de estudar o efeito da aplicação do PRP no sitio doador de enxerto do ligamento da patela para reconstrução do ligamento cruzado anterior (LCA), realizamos ressonância magnética aos seis meses de pós-operatório para avaliar o ligamento da patela. Também aplicamos questionários de função do joelho e realizamos teste isocinético após seis meses da cirurgia. MÉTODOS: O presente estudo prospectivo, randomizado, avaliador cego incluiu 27 pacientes. Comparamos doze pacientes em que foi utilizado o PRP no defeito criado no terço central do ligamento da patela para retirada de enxerto com quinze pacientes do grupo controle. Aos seis meses de cirurgia realizamos ressonância magnética, em que foi avaliada a área não regenerada do defeito no terço central do ligamento da patela, a área de secção transversa do ligamento e a altura da patela pelo índice de Insall- Salvati. Realizamos teste isocinético e aplicamos questionários específicos de função do joelho. Quantificamos a dor pós-operatória imediata com escala visual analógica (EVA) de dor. RESULTADOS: Aos seis meses de pós-operatório a área não regenerada do defeito no terço central do ligamento da patela foi 4,95 mm2 no grupo PRP e 9,38 mm2 no grupo controle (p=0,046, teste t de Student). A área de secção transversa do ligamento da patela no grupo PRP foi de 173,05 mm2 e no grupo controle 176,29 mm2 (p=0,856). O índice de Insall-Salvati nos grupos PRP e controle foi de 1,04 e 1,06, respectivamente (p=0,808). Não houve diferença entre os grupos nos resultados dos questionários e teste isocinético. Nos pacientes em que foi utilizado o PRP houve menos dor pós-operatória imediata (p=0,02). CONCLUSÃO: Concluímos, nessa amostra, que o uso do PRP no defeito do terço central do ligamento da patela na reconstrução do LCA, nos primeiros seis meses; determinou maior regeneração do defeito; diminuiu a intensidade da dor pós-operatória imediata; não ocasionou alterações estruturais na avaliação por ressonância magnética; e não modificou os resultados clínicos e funcionais da cirurgia / INTRODUCTION: Platelet-rich plasma (PRP) has been used in sports medicine to improve and accelerate ligaments and tendons healing, in an effort to provide a faster return to sports activities. However there is a lack of high evidence level studies to support its use. With the purpose to study the effect of PRP, patellar ligament was evaluated six months after harvesting its central third with magnetic resonance imaging (MRI). We also applied specific questionnaires of knee function and isokinetic testing. METHODS: This blinded, randomized, prospective study included 27 patients. Twelve patients in whom we added PRP in the patellar ligament after harvesting its central third for ACL reconstruction were compared to fifteen patients in the control group. After six months we evaluated the non-regenerated area of the patellar ligament, the cross section area of the patellar ligament and its length (Insall-Salvati index) with MRI. We also evaluated the results of isokinetic testing and specific questionnaires of knee function. Immediate post-operative pain was evaluated with visual analogical scale (VAS) score. RESULTS: The non-regenerated area of the patellar ligament was 4,95 mm2 in the PRP group and 9,35 mm2 in the control group (p=0,046 test). The cross section area of the patellar ligament in the PRP group was 173,05 mm2 and in the control group 176,29 mm2 (p=0,856). The Insall- Salvati index in the PRP and control group was 1,04 and 1,06, respectively (p=0,806). There was no difference between the groups in the questionnaires and isokinetic testing results. PRP group patients had less post-operative pain (p=0,02). CONCLUSION: We concluded, in this sample, that the use of PRP in the patellar ligament after harvesting its central third for ACL reconstruction, in the first six months: improved the ligament regeneration; reduced the intensity of immediate post-operative pain; did not cause structural changes in the patellar ligament in MRI; and did not modify the clinical and functional results of the procedure

The stringent requirement for hemopoietic growth factors (HGF) in the induction of hemopoiesis in vitro has raised questions as to their possible role(s) in leukemogenesis. Several recent clinical studies have shown aberrant cell growth factor gene activation in patient derived leukemic cells. Assessment of growth factor activity is often based on in vitro bioactivity assays of conditioned media or body fluids. The specificity of this type of endpoint is, however, open to question due to the overlap in biological activities of many HGFs. In assessing the role of growth factor gene expression in a murine myeloid leukemia model I have used a sensitive RNA detection procedure coupled with a vector-probe system that enables the synthesis of uniformly labelled radioactive DNA probes to detect unambiguously the expression of particular growth factor genes. The Abelson murine leukemia virus (A-MuLV) derived myeloid transformants used in this study had previously been shown to produce a multi-lineage colony stimulating activity (CSA). While these A-MuLV transformants were shown to produce GM-CSF, it seemed likely that the multi-lineage CSA was due to another factor. In addition to confirming the expression of GM-CSF mRNA, I was able to show that the cells of all four A-MuLV transformed lines tested also expressed interleukin-3 mRNA. This finding was strongly corroborated by bio-activity data obtained using the CM from the A-MuLV myeloid transformants. Additional preliminary analysis by bioactivity assays have also shown the possible presence of interleukin-6 (IL-6) and a recently described pre-B cell factor suggesting perhaps a common mechanism underlying the activation of these various growth factor genes. / Medicine, Faculty of / Medical Genetics, Department of / Graduate

Indiana University-Purdue University Indianapolis (IUPUI) / Endodontic treatment of the infected immature tooth has undergone a dramatic change. Conventional endodontic treatment can control infection, but root development usually remains impaired. A novel regenerative endodontic procedure, the revascularization method, can now control the infection and enable such teeth to continue root development. This is done by creating a fibrin-matrix scaffold in the antibiotic treated root canal space (RCS). Dental stem cells and growth factors have been able to continue root development in such an environment. The fibrin-matrix scaffold is dependent on the induction of a blood clot into the RCS, and this cannot always be predictably induced. PDS is a biocompatible material that can be electrospun to provide a matrix for cells and growth factors and perhaps improve on the blood clot induced fibrin scaffold by incorporating metronidazole as an adjuvant antimicrobial. A metronidazole containing electrospun PDS scaffold was examined in vitro using a turbidimetric test, the modified direct contact test. This scaffold significantly inhibited growth of an anaerobic primary endodontic pathogen Porphyromonas gingivalis. This scaffold may improve the treatment of the infected immature tooth by providing a designed matrix for root regeneration while serving simultaneously as an antibiotic drug delivery device to disinfect the RCS. The aim of this study is to evaluate in vitro the property of a synthetic scaffold to function as an antibacterial drug delivery device. PDS*II (polydioxanone) suture was obtained from Ethicon, INC. (Somerville, NJ) and was dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol, HFIP (Sigma Aldrich). Three different scaffolds were electrospun onto an aluminum foil background; (1) control scaffold with no antibiotic incorporated, (2) scaffold with 5.0-wt % metronidazole incorporated, and (3) 25-wt % metronidazole incorporated. All scaffolds were cut using a 4-mm diameter biopsy punch under aseptic conditions and removed from foil, control scaffold (n = 64), scaffold containing 5.0-wt % metronidazole (n = 32), and scaffold containing 25-wt % metronidazole (n=32). Experimental scaffolds were placed in a 96- well sterile flat bottom microtiter plate. Porphyromonas gingivalis a known primary endodontic pathogen was grown in 5 ml of BHI + YE with 0.25 μl of vitamin K with incubation at 37°C under anaerobic conditions for 48 hours. Microplates were sterilized before inoculation with Pg with 400 μl of 70-percent EtOH for a minimum of 30 minutes then pipetted out. After sterilization the microwells were washed with 400 μl of sterile water and pipetted out. Group 1 (negative control) microwells (n = 8) contained control scaffold and 190 μl of broth only. Group 2 (positive control) microwells (n = 8) contained 190 μl of broth and Pg only. Group 3 microwells (n = 8) contained control scaffold, 190 μl of broth, and 10 μl of Pg inoculum. Group 4 microwells (n = 8) contained scaffold with 5 wt % metronidazole, 190 μl of broth, and 10 μl of Pg inoculum. Group 5 microwells (n = 8) contained scaffold with 25 wt % metronidazole, 190 μl of broth, and 10 μl of Pg inoculum. Group 6 contained 190 μl of uninoculated broth for spectrophotometer calibration. Sterile microplate lids were used to isolate microwells from the surrounding environment. Microplates were incubated at 37°C under anaerobic conditions for 48 hours. After 48 hours the microplates were read by using an endpoint reading in the spectrophotometer. This was repeated four times. Comparisons among the groups for differences in optical density as a measure of bacterial growth were made using mixed-model ANOVA, with a fixed effect for group and a random effect for experimental run. Pair-wise group comparisons were performed using Tukey's multiple comparisons procedure to control the overall significance level at 5 percent. The analyses were performed using the ranks of the data. Broth had significantly lower OD than all other groups (p < 0.0001). Broth+Pg and Broth+Pg+Scaffold had significantly higher OD than 5-wt % Metro (p < 0.0001) and 25-wt % Metro (p < 0.0001), but Broth+Pg and Broth+Pg+Scaffold were not significantly different from each other (p = 0.97). 5-wt % Metro and 25-wt % Metro were not significantly different from each other (p = 0.24). From the results of our study, we concluded that the 5.0-wt % and 25-wt % metronidazole containing scaffolds significantly inhibited bacterial growth and could be effectively utilized for the endodontic regeneration procedure.

Introdução: Arginina e antioxidantes estão associados à melhora funcional de cicatrização. Formulação enteral suplementada com arginina e antioxidantes tem sido proposta para corrigir déficit nutricional e garantir substratos ideais para uma boa cicatrização. Ainda não existem informações disponíveis sobre os possíveis mecanismos envolvidos. Objetivo: Avaliar o efeito da nutrição enteral suplementada com arginina e antioxidantes sobre o processo de cicatrização de feridas cutâneas em ratos nutridos e previamente desnutridos, em termos de avaliação morfo-estrutural, bioquímica e biologia molecular. Método: Ratos isogênicos, machos, adultos com peso entre 250 a 350 g, foram divididos aleatoriamente em seis grupos. Três grupos foram mantidos nutridos com alimentação com dieta padrão AIN-93M e três grupos foram submetidos ao regime de desnutrição por 14 dias, com perda de peso corpóreo entre 12 e 15% em relação ao peso corpóreo inicial. Após esse período, os grupos de ratos nutridos e os previamente desnutridos, foram submetidos à lesão cutânea dorsal padronizada e gastrostomia. A seguir, os ratos receberam aleatoriamente dieta por via oral, dieta enteral padrão ou dieta enteral suplementada com arginina e antioxidantes por via gastrostomia, durante 14 dias pós-trauma (PT). A área da lesão cutânea no dia do trauma, no 7º e 14º dias PT foram medidas por fotografia digital. No 7º e 14º dias PT, em tecido de granulação cicatricial, por meio de análise histológica, foram avaliadas as variáveis reepitelização, infiltrado inflamatório, recomposição da derme, quantificação do colágeno total e miofibroblastos (imunoistoquímica). Amostras do tecido de granulação, retiradas no 7º dia PT, foram submetidas à análise de expressão gênica de fatores de crescimento (TGF-beta, KGF, PDGF, VEGF) e colágenos (tipo I e III). Resultados: Ratos nutridos apresentaram maior fechamento da lesão cutânea quando comparados aos previamente desnutridos, no 7º e 14º dias PT, independente dos diferentes tipos de dieta administrados por via gastrostomia. No 14º dia PT, ratos nutridos apresentaram maior reepitelização, intensidade de infiltrado inflamatório e recomposição da derme, quando comparados aos ratos previamente desnutridos, independente da oferta de dieta por via oral, dieta enteral padrão e suplementada. Ratos nutridos e previamente desnutridos, no 7º e 14º dias PT, não apresentaram diferença na quantidade de colágeno total e miofibroblastos, independente do tipo de dieta enteral administrada por via gastrostomia. No 7º dia PT, ratos nutridos apresentaram aumento na expressão gênica dos fatores de crescimento TGF-beta e KGF e colágenos I e III, quando comparados aos ratos previamente desnutridos, independente da dieta enteral administrada por via gastrostomia. Conclusões: 1 - Com estado nutricional mantido, a cicatrização ocorre de maneira adequada, independente da dieta oral, enteral padrão ou suplementada. 2- A desnutrição retarda a cicatrização em termos de epitelização, recomposição da derme e contração da ferida cutânea, independente da realimentação com dieta oral, enteral padrão ou suplementada. 3- Após uma semana de trauma cutâneo, a expressão gênica de fatores de crescimento ligados à cicatrização apresentaram-se alterados em virtude da desnutrição prévia, e não foram revertidos independentemente da realimentação com dieta oral, enteral padrão ou suplementada. 4- Após uma semana de trauma cutâneo, a expressão gênica dos colágenos tipo I e III ligados à cicatrização apresentaram-se alterados em virtude da desnutrição prévia, e não foram revertidos independentemente da realimentação com dieta oral, enteral padrão ou suplementada / Introduction: Arginine and antioxidants are associated with functional enhancement of healing. Arginine and antioxidants supplemented enteral formulas have been used to revert nutritional deficits and to guarantee substrates to ideal healing. The possible mechanisms involved have not been totally elucidated. Objective: To examine the effect of enteral nutrition supplemented with arginine and antioxidants on cutaneous wound healing process in nourished and previously malnourished rats in morphological structural, biochemical and molecular analyses. Methods: Isogenic rats, male, adults, weighting 250 to 350 g, were divided in six groups. Three groups were maintained nourished with oral diet AIN-93 and three groups were submitted to malnutrition process for 14 days, with 12 to 15% of body weight loss. Nourished and previously malnourished groups were submitted to dorsal cutaneous wound and gastrostomy. The rats received oral diet, standard enteral diet or enteral diet supplemented with arginine and antioxidants through gastrostomy during 14 days post trauma (PT). The cutaneous wound area on day of trauma, 7th and 14th days post-trauma were calculated. At 7th and 14th day, histological variables (re-epithelization, inflammatory infiltrate, dermal recomposition and total collagen quantification) and myofibroblasts were analyzed at granulation tissue. Growth factors (TGF-beta, KGF, PDGF and VEGF) and collagens (type I and III) gene expression analyses were performed at samples from the granulation tissue. Results: Nourished rats showed higher contraction of cutaneous wound when compared with previously malnourished rats, on 7th and 14th days post trauma (PT) independent of different enteral diet administered through gastrostomy. On 14th day PT, nourished rats showed higher re-epithelization, inflammatory infiltrate intensity and dermal recomposition when compared to previously malnourished rats, independent of physiologic solution, standard enteral diet and supplemented enteral diet with arginine and antioxidants. Total collagen quantification and myofibroblasts semi-quantification, did not show any significant difference, independent of the enteral diet type, administered through gastrostomy in nourished and previously malnourished rats, at 7th and 14th days PT. Nourished rats showed higher levels of TGF-beta, KGF, collagen type I and III gene expression when compared to previously malnourished rats, independent of the enteral diet type administered through gastrostomy at the 7th day PT. Conclusions: 1- Adequate healing process occurs with the maintenance of nutritional status, independent of the feeding of a oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants, 2- Previous malnutrition state slower re-epithelization, dermal recomposition and contraction, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants refeeding. 3- Previous malnutrition reduce the levels of growth factors gene expression involved on wound healing, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants after seven days post-trauma. 4- Previous malnutrition reduce the levels of collagens type I and III gene expression involved on wound healing, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants after seven days post-trauma

Made available in DSpace on 2014-06-11T19:28:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-03-27Bitstream added on 2014-06-13T19:57:15Z : No. of bitstreams: 1 esper_la_me_araca.pdf: 415604 bytes, checksum: 36bcb3441620df6465aef668552a719f (MD5) / Este estudo avaliou histomorfometricamente o processo de cicatrização em defeitos de fenestração periodontal, criados cirurgicamente em cães e tratados com Plasma Rico em Plaquetas (PRP) associado ou não ao uso de barreira de membrana. Defeitos de fenestração periodontal com 5 mm de diâmetro foram cirurgicamente criados nos caninos superiores de 12 cães. Os dentes foram divididos em 4 grupos: C (controle) - defeito preenchido somente com coágulo sangüíneo; M - defeito preenchido com coágulo sangüíneo e protegido com uma membrana de politetrafluoretileno expandido (PTFE-e) com reforço de titânio; PRP - defeito preenchido com PRP; PRP/M - defeito preenchido com PRP e protegido com uma membrana de PTFE-e com reforço de titânio. Os animais foram eutanasiados após 4 semanas. Medidas lineares e de área da cicatrização periodontal foram avaliadas e calculadas como porcentagem do defeito original. Os dados foram submetidos à análise estatística (análise de variância, p < 0,05). Nenhum espécime regenerou-se completamente com osso ou cemento. Formação de novo cemento foi significativamente maior nos Grupos PRP e PRP/M quando comparados ao Grupo C. Observou-se, também, significativa maior formação de novo cemento no Grupo PRP/M que no Grupo M. Dentro dos limites deste estudo, pode-se concluir que o PRP favoreceu a formação de novo cemento. A Regeneração Tecidual Guiada (RTG) não proporcionou efeitos adicionais ao uso do PRP no tratamento de defeitos de fenestração periodontal em cães. / This study histomorphometrically analyzed the healing of periodontal fenestration defects surgically created in dogs and treated with Platelet-Rich Plasma (PRP) with or without a barrier membrane. A 5 mm diameter periodontal fenestration defect was made in each upper canine of 12 dogs. The teeth were divided into 4 groups: C (control) - defect filled with blood clot only; M - defect filled with blood clot and covered by a titanium-reinforced expanded polytetrafluoroethylene membrane (ePTFE); PRP - defect filled with PRP; PRP/M - defect filled with PRP and covered by a titanium-reinforced ePTFE. All animals were euthanized at 4 weeks post-operative. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Data were statistically analyzed (analysis of variance, p < 0.05). No defect completely regenerated with either bone or cementum. Cementum formation was significantly greater in groups PRP and PRP/M when both groups were compared to Group C. A significant greater cementum formation was also observed in Group PRP/M than in Group M. Within the limits of this study, it can be concluded that the PRP favored cementum formation. Guided Tissue Regeneration (GTR) did not promote any additional benefit to the use of PRP in the treatment of periodontal fenestration defects in dogs.

Introdução: Arginina e antioxidantes estão associados à melhora funcional de cicatrização. Formulação enteral suplementada com arginina e antioxidantes tem sido proposta para corrigir déficit nutricional e garantir substratos ideais para uma boa cicatrização. Ainda não existem informações disponíveis sobre os possíveis mecanismos envolvidos. Objetivo: Avaliar o efeito da nutrição enteral suplementada com arginina e antioxidantes sobre o processo de cicatrização de feridas cutâneas em ratos nutridos e previamente desnutridos, em termos de avaliação morfo-estrutural, bioquímica e biologia molecular. Método: Ratos isogênicos, machos, adultos com peso entre 250 a 350 g, foram divididos aleatoriamente em seis grupos. Três grupos foram mantidos nutridos com alimentação com dieta padrão AIN-93M e três grupos foram submetidos ao regime de desnutrição por 14 dias, com perda de peso corpóreo entre 12 e 15% em relação ao peso corpóreo inicial. Após esse período, os grupos de ratos nutridos e os previamente desnutridos, foram submetidos à lesão cutânea dorsal padronizada e gastrostomia. A seguir, os ratos receberam aleatoriamente dieta por via oral, dieta enteral padrão ou dieta enteral suplementada com arginina e antioxidantes por via gastrostomia, durante 14 dias pós-trauma (PT). A área da lesão cutânea no dia do trauma, no 7º e 14º dias PT foram medidas por fotografia digital. No 7º e 14º dias PT, em tecido de granulação cicatricial, por meio de análise histológica, foram avaliadas as variáveis reepitelização, infiltrado inflamatório, recomposição da derme, quantificação do colágeno total e miofibroblastos (imunoistoquímica). Amostras do tecido de granulação, retiradas no 7º dia PT, foram submetidas à análise de expressão gênica de fatores de crescimento (TGF-beta, KGF, PDGF, VEGF) e colágenos (tipo I e III). Resultados: Ratos nutridos apresentaram maior fechamento da lesão cutânea quando comparados aos previamente desnutridos, no 7º e 14º dias PT, independente dos diferentes tipos de dieta administrados por via gastrostomia. No 14º dia PT, ratos nutridos apresentaram maior reepitelização, intensidade de infiltrado inflamatório e recomposição da derme, quando comparados aos ratos previamente desnutridos, independente da oferta de dieta por via oral, dieta enteral padrão e suplementada. Ratos nutridos e previamente desnutridos, no 7º e 14º dias PT, não apresentaram diferença na quantidade de colágeno total e miofibroblastos, independente do tipo de dieta enteral administrada por via gastrostomia. No 7º dia PT, ratos nutridos apresentaram aumento na expressão gênica dos fatores de crescimento TGF-beta e KGF e colágenos I e III, quando comparados aos ratos previamente desnutridos, independente da dieta enteral administrada por via gastrostomia. Conclusões: 1 - Com estado nutricional mantido, a cicatrização ocorre de maneira adequada, independente da dieta oral, enteral padrão ou suplementada. 2- A desnutrição retarda a cicatrização em termos de epitelização, recomposição da derme e contração da ferida cutânea, independente da realimentação com dieta oral, enteral padrão ou suplementada. 3- Após uma semana de trauma cutâneo, a expressão gênica de fatores de crescimento ligados à cicatrização apresentaram-se alterados em virtude da desnutrição prévia, e não foram revertidos independentemente da realimentação com dieta oral, enteral padrão ou suplementada. 4- Após uma semana de trauma cutâneo, a expressão gênica dos colágenos tipo I e III ligados à cicatrização apresentaram-se alterados em virtude da desnutrição prévia, e não foram revertidos independentemente da realimentação com dieta oral, enteral padrão ou suplementada / Introduction: Arginine and antioxidants are associated with functional enhancement of healing. Arginine and antioxidants supplemented enteral formulas have been used to revert nutritional deficits and to guarantee substrates to ideal healing. The possible mechanisms involved have not been totally elucidated. Objective: To examine the effect of enteral nutrition supplemented with arginine and antioxidants on cutaneous wound healing process in nourished and previously malnourished rats in morphological structural, biochemical and molecular analyses. Methods: Isogenic rats, male, adults, weighting 250 to 350 g, were divided in six groups. Three groups were maintained nourished with oral diet AIN-93 and three groups were submitted to malnutrition process for 14 days, with 12 to 15% of body weight loss. Nourished and previously malnourished groups were submitted to dorsal cutaneous wound and gastrostomy. The rats received oral diet, standard enteral diet or enteral diet supplemented with arginine and antioxidants through gastrostomy during 14 days post trauma (PT). The cutaneous wound area on day of trauma, 7th and 14th days post-trauma were calculated. At 7th and 14th day, histological variables (re-epithelization, inflammatory infiltrate, dermal recomposition and total collagen quantification) and myofibroblasts were analyzed at granulation tissue. Growth factors (TGF-beta, KGF, PDGF and VEGF) and collagens (type I and III) gene expression analyses were performed at samples from the granulation tissue. Results: Nourished rats showed higher contraction of cutaneous wound when compared with previously malnourished rats, on 7th and 14th days post trauma (PT) independent of different enteral diet administered through gastrostomy. On 14th day PT, nourished rats showed higher re-epithelization, inflammatory infiltrate intensity and dermal recomposition when compared to previously malnourished rats, independent of physiologic solution, standard enteral diet and supplemented enteral diet with arginine and antioxidants. Total collagen quantification and myofibroblasts semi-quantification, did not show any significant difference, independent of the enteral diet type, administered through gastrostomy in nourished and previously malnourished rats, at 7th and 14th days PT. Nourished rats showed higher levels of TGF-beta, KGF, collagen type I and III gene expression when compared to previously malnourished rats, independent of the enteral diet type administered through gastrostomy at the 7th day PT. Conclusions: 1- Adequate healing process occurs with the maintenance of nutritional status, independent of the feeding of a oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants, 2- Previous malnutrition state slower re-epithelization, dermal recomposition and contraction, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants refeeding. 3- Previous malnutrition reduce the levels of growth factors gene expression involved on wound healing, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants after seven days post-trauma. 4- Previous malnutrition reduce the levels of collagens type I and III gene expression involved on wound healing, independent of refeeding with oral diet, standard enteral diet or supplemented enteral diet with arginine and antioxidants after seven days post-trauma