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Synthetic Resveratrol Aliphatic Acid Inhibits tlr2-Mediated Apoptosis and an Involvement of Akt/GSK3β PathwayChen, Lin, Zhang, Yi, Sun, Xiuli, Li, Hui, LeSage, Gene, Javer, Avani, Zhang, Xiumei, Wei, Xinbing, Jiang, Yulin, Yin, Deling 01 July 2009 (has links)
As resveratrol derivatives, resveratrol aliphatic acids were synthesized in our laboratory. Previously, we reported the improved pharmaceutical properties of the compounds compared to resveratrol, including better solubility in water and much tighter binding with human serum albumin. Here, we investigate the role of resveratrol aliphatic acids in Toll-like receptor 2 (TLR2)-mediated apoptosis. We showed that resveratrol aliphatic acid (R6A) significantly inhibits the expression of TLR2. In addition, overexpression of TLR2 in HEK293 cells caused a significant decrease in apoptosis after R6A treatment. Moreover, inhibition of TLR2 by R6A decreases serum deprivation-reduced the levels of phosphorylated Akt and phosphorylated glycogen synthase kinase 3β (GSK3β). Our study thus demonstrates that the resveratrol aliphatic acid inhibits cell apoptosis through TLR2 by the involvement of Akt/GSK3β pathway.
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Glycogen Synthase Kinase-3 and p38 MAPK Are Required for Opioid-Induced Microglia ApoptosisXie, Nanchang, Li, Hui, Wei, Dailin, LeSage, Gene, Chen, Lin, Wang, Shengjun, Zhang, Yi, Chi, Lingyi, Ferslew, Kenneth, He, Lei, Chi, Zhaofu, Yin, Deling 01 November 2010 (has links)
Opioids have been widely applied in clinics as one of the most potent pain relievers for centuries, but their abuse has deleterious physiological effects beyond addiction. We previously reported that opioids inhibit cell growth and trigger apoptosis in lymphocytes. However, the underlying mechanism by which microglia apoptosis in response to opioids is not yet known. In this study, we show that morphine induces microglia apoptosis and caspase-3 activation in an opioid-receptor dependent manner. Morphine decreased the levels of microglia phosphorylated Akt (p-Akt) and p-GSK-3β (glycogen synthase kinase-3 beta) in an opioid-receptor dependent manner. More interestingly, GSK-3β inhibitor SB216763 significantly increases morphine-induced apoptosis in both BV-2 microglia and mouse primary microglial cells. Moreover, co-treatment of microglia with SB216763 and morphine led to a significant synergistic effect on the level of phospho-p38 mitogen-activated protein kinase (MAPK). In addition, inhibition of p38 MAPK by its specific inhibitor SB203580 significantly inhibited morphine-induced apoptosis and caspase-3 activation. Taken together, our data clearly demonstrates that morphine-induced apoptosis in microglial cells, which is mediated via GSK-3β and p38 MAPK pathways.
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The Novel Role of Interleukin-1 Receptor-Associated Kinase 1 in the Signaling Process Controlling Innate Immunity and InflammationFang, Youjia 29 May 2009 (has links)
Obesity-induced chronic inflammation plays a key role in the pathogenesis of insulin resistance and the metabolic syndrome. Proinflammatory cytokines can cause insulin resistance in adipose tissue, skeletal muscle and liver by inhibiting insulin signaling transduction. Interleukin-1 receptor-associated kinase-1 (IRAK-1) is a serine/threonine kinase functioning in Toll-like Receptor signaling pathways, and plays an important role in inflammation and immune response. In our studies, we demonstrated that IRAK-1 is involved with the negative regulation of PI3K-Akt dependent signaling pathway induced by insulin and TLR 2&4 agonists. Out data also indicate that IRAK-1 can interact with IRS-1 protein both in vivo and in vitro. The binding sites for the IRAK1-IRS1 biochemical interaction are IRS-1's PH domain and IRAK-1's proline-rich LWPPPP motif. Our studies also indicate that IRAK-1 is involved with the negative regulation of glycogen synthesis through inhibiting PI3K-Akt signaling pathway and thus releasing GSK3β's inhibitory effect on glycogen synthase. Moreover, our studies also suggest that IRAK-1 is involved in the activation of transcription factors CREB and ATF-1 by stimulating CREB-Ser133 and ATF-1 phosphorylation. CREB transcription factor family induces genes involved in cellular metabolism, gene transcription, cell cycle regulation, cell survival, as well as growth factor and cytokine genes. That may partially explain our finding that IRAK-1 may be also involved with cell proliferation and survival pathway. / Master of Science
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Chronic Morphine Effect on Inflammatory Cytokine Production in Activated BV-2 Microglia Cell Line via Akt/Gsk3β SignalingMakinwa, Yetunde R 01 May 2016 (has links)
The pathophysiology of chronic morphine on the immune system, especially on the cells of the innate immune system that leads to an immune compromise state has not been fully elucidated. The cells of the innate immune system are the first line of defense in mounting an immune response needed in infections, inflammation, cancer development, etc. One of the ways by which these innate immune cells act is by the production of cytokines with direct effects and to also recruit other immune cells, as required. A balance of pro- and anti-inflammatory cytokines is necessary for immune competence. I hypothesized that chronic morphine would act via a classical opioid receptor to stimulate the PI3K/Akt/Gsk3β pathway to produce predominantly anti-inflammatory cytokines. Cytokine gene expression levels were assessed via RT-PCR; Akt and Gsk3β protein levels measured using indirect ELISA. The data suggests that chronic morphine causes a significant reduction in IL-6 production, but does not act via the Akt/Gsk3β pathway or the classical opioid receptor to cause this effect in microglia cells.
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Implication du Régulateur endogène de la Calcineurine 1 dans la transmission et la plasticité synaptiqueDudilot, Anthony 08 1900 (has links)
Le régulateur endogène de la calcineurine 1 (RCAN1) est exprimé dans les neurones, cependant son rôle dans la régulation de la transmission et de la plasticité synaptique est mal connu. De manière intéressante, plusieurs études dans les cellules cardiaques et les levures montrent une double régulation de la calcineurine 1 (CaN1) par RCAN1. Il est décrit qu'en fonction de son état de phosphorylation par la kinase glycogène synthase 3β (GSK3β), RCAN1 réprime la CaN1 à l'état déphosphorylé, mais il faciliterait son activité à l'état phosphorylé. La régulation de la CaN1 par RCAN1 phosphorylé n'a encore jamais été étudiée dans les neurones et pourrait mettre en relation deux acteurs majeurs de la dépression à long terme (LTD), à savoir la CaN1 et la GSK3β. Par ailleurs une étude récente a montré que RCAN1 peut également être phosphorylé par la protéine kinase A (PKA), une kinase essentielle dans la mise en place de la potentialisation à long terme (LTP), entrainant ainsi une augmentation de l’inhibition de la CaN1 par RCAN1. Dans les neurones, RCAN1 pourrait donc potentiellement réguler à la fois la LTP et la LTD dépendamment de son état de phosphorylation. Mes travaux visent à élucider si RCAN1 est capable de réguler la transmission et de la plasticité synaptique en fonction de son état de phosphorylation et si son action dépend de la CaN1.
Afin de déterminer le rôle de RCAN1 dans ces processus, une combinaison de techniques de biologie moléculaire, d’électrophysiologie et d'imagerie a été employée. Nous avons généré des mutations ponctuelles de RCAN1 sauvage de manière à rendre RCAN1 non phosphorylable par la GSK3β ou la PKA. L’expression virale de RCAN1 et de ses différents mutants dans des cultures primaires de neurones d’hippocampe a révélé que RCAN1, dans ses versions sauvage et mutées, est localisé au niveau des épines dendritiques, suggérant une possible fonction de RCAN1 à la synapse.
De manière à déterminer les effets de RCAN1 sur la transmission et la plasticité synaptique, j’ai exprimé de manière virale RCAN1 et ses différents mutants dans des tranches organotypiques d'hippocampes de rat et analysé leurs effets par enregistrement en ‘‘patch-clamp’’ en configuration de cellule entière. J’ai pu observer que le blocage du site de phosphorylation de RCAN1 par la GSK3β entraînait une augmentation de la transmission synaptique ainsi qu’un blocage de la LTD. De plus j’ai démontré que la LTP été bloquée lorsque la PKA ne pouvait pas phosphoryler RCAN1. Enfin nous avons pu déterminer que ces différents effets de RCAN1 sur la transmission et la plasticité synaptique étaient dépendants de la CaN1. Nous avons donc démontré une cascade d’évènements et mis en évidence le rôle clé de RCAN1 dans la régulation de la LTP et de la LTD. Nous proposons donc que RCAN1 permet de moduler la transmission et la plasticité synaptique en fonction de son état de phosphorylation par la GSK3β et la PKA en agissant sur la CaN1, en étant un effecteur de la GSK3β lors de l’induction de la LTD ainsi qu’un effecteur de la PKA lors de l’induction de la LTP. / The endogenous regulator of calcineurin 1 (RCAN1) is expressed in neurons, nevertheless its role in the regulation of synaptic transmission and plasticity is not well understood. Interestingly, several studies in cardiac cells and yeasts show that RCAN1 is able to inhibit or activate CaN1 depending on its phosphorylation state by glycogen synthase kinase 3β (GSK3β). RCAN1 is able to inhibit CaN1 when it is not phosphorylated by GSK3β and able to activate it in its phosphorylated state. The regulation of CaN1 by phosphorylated RCAN1 has never been studied in neurons although it could provide a critical link between two major actors of long-term depression (LTD), CaN1 and GSK3β. Furthermore, a recent study revealed that RCAN1 can also be phosphorylated by protein kinase A (PKA), a kinase involved in regulating long-term potentiation (LTP), leading to an increase of CaN1 inhibition by RCAN1. Thus, in neurons, the differential phosphorylation of RCAN1 could potentially regulate both LTP and LTD. My work therefore investigates how, depending on its phosphorylation state, RCAN1 affects synaptic transmission and plasticity and if this occurs via a direct action on CaN1.
In order to determine the role of RCAN1 phosphorylation in synaptic plasticity, a combination of molecular biology, imaging and electrophysiology was used. We generated point mutations of wild type RCAN1 in order to obtain two non-phosphorylable forms of RCAN1: one that couldn’t be phosphorylated by GSK3β, and another one that PKA could not phosphorylate. Viral expression of RCAN1 and its phosphorylation deficient mutants in dissociated hippocampal cultures revealed that they are localized within dendritic spines, hinting at a synaptic function of RCAN1.
To determine the effects of RCAN1 on synaptic transmission and plasticity, I virally expressed RCAN1 and the phosphorylation deficient mutants of RCAN1 in rat organotypic hippocampal slice cultures and analyzed their effects on synaptic plasticity by whole cell ‘‘patch-clamp’’ recordings. I observed that the blockade of the GSK3β phosphorylation site in RCAN1 increased synaptic transmission and blocked LTD induction. Furthermore, I demonstrate that LTP was blocked when PKA was unable to phosphorylate RCAN1. Finally, I determined that these distinct effects of RCAN1 on synaptic transmission and plasticity were directly dependent on CaN1. I thus define a cascade of events as well as demonstrate the key role of RCAN1 in the regulation of both LTP and LTD. Based on my results, I propose that
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RCAN1 modulates synaptic transmission and plasticity according to its phosphorylation states by GSK3β and PKA, via its direct action on CaN1, being an effector of both GSK3β during LTD and PKA during LTP induction.
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Beta-Arrestin 2 Modulates Resveratrol-Induced Apoptosis and Regulation of Akt/GSK3β PathwaysSun, Xiuli, Zhang, Yi, Wang, Jianliu, Wei, Lihui, Li, Hui, Hanley, Gregory, Zhao, Miaoqing, Li, Yi, Yin, Deling 01 September 2010 (has links)
Background: Resveratrol is emerging as a novel anticancer agent. However, the mechanism(s) by which resveratrol exerts its effects on endometrial cancer (EC) are unknown. We previously reported that β-arrestin 2 plays a critical role in cell apoptosis. The role of β-arrestin 2 in resveratrol modulation of endometrial cancer cell apoptosis remains to be established. Scope of Review: EC cells HEC1B and Ishikawa were transfected with either β-arrestin 2 RNA interfering (RNAi) plasmid or β-arrestin 2 full-length plasmid and control vector. The cells were then exposed to differing concentrations of resveratrol. Apoptotic cells were detected by TUNEL assay. Expression of total and phosphorylated Akt (p-Akt), total and phosphorylated glycogen synthase kinase 3 beta (p-GSK3β), and caspase-3 were determined by Western blot analysis. Our data demonstrate that inhibition of β-arrestin 2 increases the number of apoptotic cells and caspase-3 activation. Additionally β-arrestin 2 exerted an additive effect on resveratrol-reduced levels of p-Akt and p-GSK3β. Overexpression of β-arrestin 2 decreased the percentage of apoptosis and caspase-3 activation and attenuated resveratrol-reduced levels of p-Akt and p-GSK3β. Taken together, our studies demonstrate for the first time that β-arrestin 2 mediated signaling plays a critical role in resveratrol-induced apoptosis in EC cells. Major Conclusions: Resveratrol primes EC cells to undergo apoptosis by modulating β-arrestin 2 mediated Akt/GSK3β signaling pathways. General significance: These inspiring findings would provide a new molecular basis for further understanding of cell apoptotic mechanisms mediated by β-arrestin 2 and may provide insights into a potential clinical relevance in EC.
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Cross-talk between insulin and serotonin signaling in the brain : Involvement of the PI3K/Akt pathway and behavioral consequences in models of insulin resistance / Dialogue entre les voies de signalisation de l’insuline et de la sérotonine dans le cerveau : Implication de la voie PI3K/Akt et conséquences comportementales dans des modèles d’insulino-résistancePapazoglou, Ioannis 04 July 2013 (has links)
L’insuline et la sérotonine (5-HT) sont deux acteurs majeurs du maintien de l’homéostasie énergétique, fonction placée sous le contrôle de l’hypothalamus. En ciblant cette région, l’insuline remplit de nombreuses fonctions métaboliques via l’activation de la voie PI3K/Akt. La 5-HT exercent des effets biologiques similaires mais les voies de signalisation impliquées dans ces processus étaient jusqu’alors mal connues. De plus, il avait été démontré que la 5-HT est capable d’activer la voie PI3K/Akt/GSK3β dans l’hippocampe, mécanisme sous-tendant potentiellement les effets antidépresseurs du neurotransmetteur. Les principaux objectifs de cette thèse étaient d’étudier 1/ l’activation de la voie PI3K/Akt par la 5-HT dans l’hypothalamus de rats diabétiques (modèle Goto-Kakizaki) et chercher un potentiel dialogue avec l’insuline and 2/ les mécanismes sous-tendant l’induction de la dépression par une alimentation hyperlipidique, par l’analyse de la phosphorylation d’Akt et GSK3β sous l’action de l’insuline, de la leptine et de la 5-HT dans l’hippocampe de rat.Ici on montre que 1/ la 5-HT stimule la voie PI3K/Akt dans l’hypothalamus et que la phosphorylation d’Akt induite par la 5-HT est atténuée dans des conditions d’insulino-résistance, suggérant l’existence d’un dialogue entre les voies de signalisation de l’insuline et de la 5-HT. Par ailleurs, nos résultats indiquent qu’une alimentation hyperlipidique induit un comportement dépressif réversible chez le rat, qui pourrait impliquer la voie PI3K/Akt/GSK3β dans les neurones subgranulaires du gyrus denté. La mise en évidence d’un dialogue entre les voies de signalisation de la 5-HT, de la leptine et de l’insuline au niveau central enrichit nos connaissances sur le rôle de ces facteurs dans la régulation de l’homéostasie énergétique et de l’humeur, et propose un lien moléculaire entre diabète de type 2, obésité et dépression / Insulin and serotonin (5-HT) are two key players in the maintenance of energy homeostasis which is controlled by the hypothalamus. In this brain region, insulin mediates numerous metabolic effects via the activation of the PI3K/Akt signaling pathway. 5-HT exerts similar biological properties by acting in the hypothalamus but the signaling pathways accountable for these effects are still unclear. Moreover, it has been reported that 5-HT induces the activation of the PI3K/Akt pathway in the hippocampus and the inhibition of GSK3β, suggesting this action as a potential mechanism for the antidepressant effects of this neurotransmitter.The main objectives of this thesis were to study 1/ the serotonin-induced activation of the PI3K/Akt in the hypothalamus of wild type and diabetic rats (Goto-Kakizaki model) and search a potential cross-talk with insulin and, 2/ the mechanisms underlying the high-fat diet induced depression by investigating the role of the phosphorylation of Akt and GSK3β by 5-HT, insulin and leptin in the hippocampus of rats.Here, we show that 5-HT triggers the PI3K/Akt signaling pathway in the rat hypothalamus, and that this activation is attenuated in insulin-resistant conditions, suggesting a cross-talk between insulin and 5-HT. Moreover, we reported that high-fat diet feeding induces a reversible depressive-like behavior, which may involve the PI3K/Akt/GSK3β pathway in subgranular neurons of the dentate gyrus. In conclusion, the activation of the PI3K/Akt pathway and its target GSK3β by 5-HT in the hypothalamus and in the dentate gyrus, respectively, can be impaired in insulin-/leptin-resistant states, which may underlie a link between metabolic diseases and depression.
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POST-TRANSLATIONAL MODIFICATION AND DEGRADATION MECHANISMS OF THE ARYL HYDROCARBON RECEPTORYang, Yujie 01 January 2021 (has links)
The aryl hydrocarbon receptor (AHR) is a transcription factor first discovered to be activated by exogenous ligands, such as dioxins, and helps promote downstream gene (e.g. CYP1A1) transcription to metabolize the toxicants. With the reports of various AHR targets genes, the expression levels and activities of AHR have been implicated in many physiological and pathological situations. Understanding how AHR protein level is regulated would provide more information to target AHR. AHR stays in the cytosol in the absence of ligand in a complex with HSP90, p23 and XAP2. After ligand activation, AHR translocates into the nucleus, fulfilling its transactivation function and then is finally degraded by proteasomes. Here, we discovered a new mechanism that controls basal AHR protein level: the selective autophagy. Loss of AHR co-chaperone p23 leads to increased protein degradation of AHR through autophagy in HeLa cells. Inhibition of autophagy using several inhibitors (chloroquine, bafilomycin A1 or 3-methyladenine) increased AHR protein levels. Knocking down of key macroautophagy protein LC3B increases AHR protein levels and decreases the responsiveness of AHR to CQ treatment. The interaction between AHR and LC3B as well as AHR and autophagy receptor p62 were confirmed in vitro and in situ. AHR is found to be lysine (K) 63-ubiquitinated in HeLa cells, which is a common signal for the autophagy-lysosomal degradation.6 We also discovered that AHR is controlled by glycogen synthase kinase 3β (GSK3β) phosphorylation. Inhibition of GSK3β activity or its expression level increased AHR protein levels while expression of HA tagged-GSK3β lowers AHR protein levels. AHR protein level is regulated through autophagy. We confirmed the GSK3β-mediated phosphorylation of AHR by phos-tag gel electrophoresis couples with Western blot analysis and identified three putative phosphorylation sites of AHR in the C-terminal half of AHR sequence. Moreover, phosphorylated AHR constitutes the active pool for transactivation and phosphorylation tagged AHR for the autophagy-lysosomal degradation, which may act as way to limit its function.
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