Thesis (Ph. D. (Psychology)) -- University of Limpopo, 2016. / Exploring the lived experiences of People Living with HIV/AIDS in relation to stigma and discrimination was the purpose of this qualitative research underpinned by Jungian Phenomenology. The study was qualitative in nature with a non-probability sample of fifteen participants aged from twenty tree to sixty four years. The setting was a rural and the research took place at a designated HIV/AIDS clinic in Limpopo. Semi-structured interviews, in the vernacular, were constructed so that the in-depth, lived experience of the participants relating to stigmatisation and discrimination could be ascertained. The participants’ responses were organised through the application of the Jungian metaphors and presented as themes which were presented in English. The archetypes of the Self, Shadow and Persona were highlighted as dominant metaphors with the Animus playing an important. These were followed by archetypes which represented more marginal roles in the participants’ personality development namely, the Anima, Hero, Caregiver, Innocent and Orphan. Everyone has the desire to develop a well-rounded socially acceptable and fulfilling personality, whose development process is accompanied by the generation and maturation of various archetypal traits. Once this is achieved, the process of personal individuation is completed and an individual is able to experience their true Self and live a fulfilling and authentic (honest) life. HIV infection and HIV related stigma generate archetypal traits which tend to impede the personality development of individuals. As a result they were not able to merge their personality development into an integrated unit and emerge as spiritual and deep-thinking beings.

MSc (Microbiology) / Department of Microbiology

Department of Public Health / MCur

MAAS / M.E.R. Mathivha Centre for Languages Arts and Culture

See the attached abstract below

MSc (Microbiology) / Department of Microbiology / See the attached abstract below.

MSCAGR (Agricultural Economics) / Department of Agricultural Economics and Agribusiness / See the attached abstract below

MSc (Microbiology) / Department of Microbiology / See the attached abstract below

PhD (Microbiology) / Department of Microbiology / High genetic diversity is a major contributory factor in the development of drug resistance, in addition to challenges in diagnosis and treatment monitoring in the therapeutics of human immunodeficiency virus (HIV) .Within the wide HIV-1 diversity, differences in mutational frequency, disease progression, drug response and transmission amongst HIV-1 subtypes have been shown. In spite HIV-1 subtype C (HIV-1C) being the most prevalent variant globally, none of the available drugs nor screening assays for inhibitory molecules have been developed targeting the genetics of this important subtype. This study therefore aimed to overexpress and biophysically characterize HIV-1C reverse transcriptase and protease to serve as reagents in the development of assays for routine screening of molecules inhibitory to HIV-1C. Heterologous expression of HIV-1C reverse transcriptase and protease isolates that are prevalent in South Africa was carried out in Escherichia coli (E. coli (BL21-DE3). The secondary and tertiary structures of the proteins were determined using, circular dichroism (CD) and fluorescence spectroscopy respectively. Thereafter, interaction studies to delineate interaction properties of natural products for possible inhibition of protease were conducted. Furthermore, in silico studies to determine binding interactions, further confirmed by in vitro binding assays of a pepsin inhibitor homolog (Bm-33) from Brugia malayi , against protease were also conducted. Expressed reverse transcriptase and protease from the globally prevalent HIV-1C were shown to be structurally and functionally intact for application in downstream HIV-1 inhibition assays. Interaction studies on the other hand revealed successful inhibition of the expressed HIV-1C PR with gallotanin. Furthermore, binding interactions of Bm-33 and HIV-1 PR revealed the first intermolecular interactions of the two molecules displaying possible inhibition of HIV-1 PR / NRF

MPH / Department of Public Health / Background: Human Immunodeficiency virus (HIV) and Acquired Immunodeficiency Syndrome (AIDS) is one of the major public health problems that has negatively impacted mostly orphans leaving in child-headed families in the world. A child- headed family is defined as a family, living under the same roof, which is headed by a person under the age of 18 years. Many of these children have become heads of households and they are obliged to look after themselves and siblings. As a result, some children drop out from school while looking for jobs to sustain the family. Purpose: The purpose of the study is to explore and describe the experiences of children in HIV/AIDS-affected child-headed families in Zimbabwe. Methodology: The researcher employed qualitative approach with explorative, descriptive, contextual and phenomenological designs. Nonprobability purposive sampling was used to select the participant. Children aged between 12-18 years were selected as they were able to narrate their life experiences. In-depth face to face individual interviews were used to collect data pertaining to experiences of children in HIV/AIDS child-headed families. Data was transcribed verbatim, and then translated from Shona to English. The data was then analysed following Tesch’s eight steps model. Findings: The findings of the study revealed that children in HIV/AIDS-affected child-headed families were lacking basic commodities such as food, clothing, shelter and clean water. The findings also revealed that children living in HIV/AIDS-affected child-headed families were being abused physically, sexually, emotionally and verbally. The findings of the study also revealed that children living in HIV/AIDS-affected child-headed families received support from the government, non-governmental organisations, community and relatives. / NRF