Sieben fibrogene und fünf nicht fibrogene (inerte) Mineralstäube wurden auf ihr Adsorptionsvermögen gegenüber Lipoproteinen untersucht. Die Adsorption erfolgte aus menschlichem Serum bzw. bovinem Lipoproteinkonzentrat. Die spezifischen Oberflächen der Stäube wurden mit Hilfe einer neuen Methode ermittelt, die auf der Adsorption von Nonadecansäure beruht. Sie vernachlässigt Oberflächenporen mit Öffnungsdurchmessern / Seven fibrogenic and five nonfibogenic (inert) mineral dusts were examined for their adsorption capacity for lipoproteins. Lipoproteins came from human serum and bovine lipoprotein- concentrate. The specific surface areas of the dusts were determined with the help of a new method, which is based on the adsorption of nonadecanoic acid. It neglects surface pores with opening diameters less than 2,5nm. The most important result is that fibrogenic dusts adsorb significantly more high density lipoproteins (HDL), than the inert dusts. The adsorption of HDL on quartz can be reduced up to 96% by addition of Polyvinylpyridine-N-oxide (PVPNO), an inhibitor of quartz- induced fibrosis. The HDL- adsorption might play a role during the developing process of mineral-dust-induced fibrosis.

Die chronische Herzinsuffizienz ist ein schwerwiegendes progredientes Krankheitsbild, das sich neben Dyspnoe und abnehmender Leistungsfähigkeit in einer nachgewiesenen Verschlechterung der HDL-Funktion manifestiert. In zahlreichen Studien, in denen der Einfluss von körperlichem Training auf die Progredienz der chronischen Herzinsuffizienz untersucht wurde, korrelierte dauerhaftes Ausdauertraining mit einer Verbesserung der eNOS-Aktivität und damit der HDLFunktion in Gefäßen. Ein Regulationsmechanismus von Endothelzellen besteht in der Expression von angiogenen microRNAs, die über negative Regulation die Proteinexpression beeinflussen. Ziel dieser Studie ist es nun, einen möglichen Zusammenhang zwischen der HDL-Funktionalität und der microRNA-Expression in Endothelzellen zu prüfen und damit die Funktionsänderung von HDL bei Herzinsuffizienten auf molekularer Ebene nachzuweisen. Zudem soll eine Beeinflussung der HDL-Funktion durch körperliches Training geprüft werden. Dafür wurde das HDL von gesunden und herzinsuffizienten Probanden (NYHAIII-Stadium) vor und nach einem vier- bzw. zwölfwöchigen Trainingsprogramm aus dem Plasma isoliert. Anschließend erfolgte mit dem gewonnenen HDL die 24-stündige Inkubation von HAEC-Kulturen. Nach Isolation der microRNAs aus dem gewonnenen Zelllysat konnte die Menge ausgewählter proangiogener miRs über RT-PCR quantifiziert werden. Die molekularbiologische Analyse der Proben zeigte eine, im Vergleich zu den Kontrollzellen, signifikant verringerte Menge an miR-21, -126 und -222 in den, mit HDLNYHAIII-inkubierten, Endothelzellen. Die miR-Expression der Endothelzellen zeigte nach dem Trainingsprogramm eine Annäherung an das Expressionsniveau der Kontrollen. Aus der dargelegten Studie wird so ersichtlich, dass das HDL von Herzinsuffizienten die Expression von proangiogenen microRNAs in Endothelzellen hemmt, was scheinbar in Korrelation mit der Ausbildung von endothelialen Dysfunktionen bei Herzinsuffizienz steht. Zudem konnte gezeigt werden, dass körperliches Training mit einer verbesserten Endothelfunktion über die Erhöhung der miR-Expression in Endothelzellen einhergeht.

A large number of proteins interact with biological membranes, either integrated in the membrane (PepTSo2), embedded on a membrane surface (5-lipoxygenase) or encircling a cutout of lipid bilayer (apolipoprotein1 (apoA-I). They function as transporters, receptors or biocatalysts in cellular processes like inflammation or cholesterol transport which are touched upon here. Malfunction of specific membrane proteins are the cause for several diseases or disorders. Knowledge of protein structure supports understanding of its mechanism of function. Here, transmission electron microscopy (TEM) was used for structure determination. To obtain structure information to high resolution for membrane proteins, normally surrounded by lipids, demands specific methods and materials for stabilization. Stabilized in detergent the structure of the bacterial transporter PepTSo2 was shown to form a tetramer even bound to substrate. However, with a protein based stabilizer, Salipro, the structure of PepTSo2 could be determined to high resolution. High density lipoprotein (HDL) in blood plasma, involved in the removal of cholesterol from peripheral tissues, have a central role in cardiovascular function, metabolic syndrome and diabetes. The HDL-particle is composed of two copies of ApoA1 and around hundred lipid molecules. From TEM data, for the first time the clearly discoidal shape could be shown by 3-dimendional reconstructions. These were used for modelling the ApoA1 protein dimer by a "biased fitting" procedure. The results indicate how ApoA1 folds around a lipid bilayer in a disc-shaped structure. Modified HDL called nanodiscs were here used to show the Ca2+ dependent binding of 5-lipoxygenase on the nanodisc bilayer and thereby increased production of the inflammatory mediator leukotrieneA4. Dimerization of 5-lipoxygenase inactivates these functions. / <p>QC 20170323</p>

Huian plasma high-density lipoprotein (HDL) were isolated by a procedure employing polyanion precipitation and column chromatography. Lipid and protein composition of the HDL isolated by this method was found to be similar to another HDL preparation isolated by ultracentrifugation. However, minor differences were noted, including a higher phospholipid and apoproteinE content and lower triglyceride content of the HDL isolated by column chromatography. Four subfraction of HDL were obtained following chromatography on an anion exchange column. The subfraction four had the highest esterified to free cholesterol ratio, the second highest phospholipid to unesterified cholesterol, and the lowest molecular weight. In addition it was consistently coincided with lecithin: cholesterol acyltransferase (LCAT) activity and found to be the best substrate for the enzyme.

INTRODUÇÃO: Apesar de classicamente a lipoproteína de alta densidade (HDL) ser descrita como um fator de risco independente para as doenças cardiovasculares (DCV), devido ao seu papel no transporte reverso de colesterol (TRC), atualmente sabe-se que esta partícula exerce múltiplos mecanismos biológicos que podem contribuir para esta cardioproteção, com estudos indo além do conteúdo de colesterol associada à HDL (HDL-C). OBJETIVO: Avaliar o impacto da suplementação de ?-3 no perfil de ácidos graxos, tamanho e capacidade antioxidante da HDL. MÉTODOS: Estudo clínico, aleatorizado, controlado, duplo cego e paralelo, onde foram incluídos 147 indivíduos adultos, com fatores de risco cardiovascular, que foram alocados no grupo ?-3 (n=77 - 3,0 g/dia contendo 1,8 g de EPA+DHA) ou no grupo ?-6 (n=70 - 3,0 g/dia de óleo de girassol contendo 1,95 g de ácido linoleico). No início (T0) e após 8 semanas (T8) amostras de sangue foram coletadas e, a partir do plasma ou soro, foram analisados o perfil lipídico (CT, HDL-C, LDL-C, TG), as apolipoproteínas AI, CII e CIII, as subfrações da HDL (Lipoprint®), a atividade da paraoxonase (PON1) e da proteína transportadora de éster de colesterol (CETP), a capacidade antioxidante da HDL (método experimental) e o perfil de ácidos graxos e conteúdo de AGNEs da HDL. Os resultados do efeito do tempo, da intervenção e das interações entre os parâmetros monitorados e os desfechos foram realizados com o auxílio do programa SPSS® versão 20.0. O valor de significância considerado foi de p<0,05. RESULTADOS: Os grupos ?-3 e ?-6 eram semelhantes entre si em relação a idade, sexo, tabagismo e uso de medicamentos, ambos apresentando alta prevalência de hipertensão e dislipidemia. Nos dois grupos, houve redução em todos os marcadores lipídicos, exceto a concentração de HDL-C, que aumentou. Observou-se o efeito do tempo no conteúdo de HDLAGNEs (?=-16,2%), no percentual de HDLGRANDE (?=20,1%) e de HDLPEQUENA (?=-5,0%). O aumento de EPA na HDL se associou à menor chance de ter elevação da atividade da PON1 (OR=0,446; IC=0,200-0,994), da concentração de HDLAGNEs (OR=0,275; IC=0,113-0,660) e do percentual de HDLPEQUENA (OR=0,337; IC=0,146-0,782). Observou-se ainda que o EPA se associou a, aproximadamente, 3,5 mais chances de aumento no percentual da HDLGRANDE (OR=3,522; IC=1,652-7,507). Quanto ao aumento de DHA na HDL, este esteve associado de maneira significativa à diminuição da concentração de Apo AI (OR=0,351; IC=0,150-0,821), além da atividade da PON1 (OR=0,226; IC=0,110-0,639) e da concentração de HDLAGNEs (OR=0,275; IC=0,113-0,668). Resultados similares aos obtidos com o EPA foram observados para o DHA e o tamanho da HDL. Não foi constatado efeito da incorporação de EPA e DHA na resistência à oxidação. CONCLUSÃO: A intervenção com ?-3 promoveu mudanças na composição da partícula de HDL, aumentando o percentual das subfrações maiores, sem, contudo, modificar sua capacidade antioxidante. / BACKGROUND: Although high-density lipoprotein (HDL) is classically described as an independent risk factor for cardiovascular disease (CVD), because of its role in reverse cholesterol transport (RCT), currently, it is known that this particle exerts multiple biological mechanisms that may contribute to this cardioprotection, with studies going beyond HDL cholesterol content (HDL-C). OBJECTIVE: To evaluate the impact of ?-3 supplementation on the fatty acid profile, size and antioxidant capacity of HDL. METHODS: A randomized, controlled, double-blind, parallel clinical study involving 147 adult subjects with cardiovascular risk factors, who were allocated into the ?-3 group (n=77 -3.0 g/day containing 1.8 g EPA + DHA) or the ?-6 group (n=70 -3.0 g/day of sunflower oil containing 1.95 g of linoleic acid). In the beginning (T0) and after 8 weeks (T8) blood samples were collected and, from plasma or serum, were analyzed lipid profile (TC, HDL-C, LDL-C, TG), apolipoproteins AI, CII and CIII, HDL subfractions (Lipoprint®), paraoxonase (PON1) and cholesterol ester carrier protein (CETP) activities, antioxidant capacity of HDL (experimental method), the fatty acid profile and NEFAs content HDL. Results of the effect of time, intervention and interactions between monitored parameters and outcomes were performed with the aid of SPSS® software version 20.0. The significance level considered was p<0.05. RESULTS: The ?-3 and ?-6 groups were similar in relation to age, sex, smoking and medication use, both presenting high prevalence of hypertension and dyslipidemia. In both groups, there was a reduction in all lipid markers except HDL-C concentration, which increased. The effect of time on HDLNEFAs content (?=-16.2%), and on the percentage of HDLLARGE (?=20.1%) and HDLSMALL (?=-5.0%) were observed. Increasing EPA in HDL was associated with a lower chance of enhance PON1 activity (OR=0.446, CI=0.200-0.994), HDLNEFAs concentration (OR=0.275, CI=0.113-0.660), and HDLSMALL (OR=0.337, CI=0.146- 0.782). It was also observed that EPA was associated with approximately 3.5 more chances of raising the percentage of HDLLARGE (OR=3.522, CI=1.652-7.507). Increasing DHA in HDL was significantly associated with a decrease in Apo AI concentration (OR=0.351, CI=0.150- 0.821), and also PON1 activity (OR=0.226, CI=0.110-0.639) and concentration of HDLNEFAs (OR=0.275, CI=0.113-0.668). Similar results obtained with EPA were observed for DHA and HDL size. No effect of the incorporation of EPA and DHA on oxidation resistance was observed. CONCLUSION: The ?-3 intervention promoted changes in the composition of the HDL particle, increasing the percentage of the larger subfractions, without, however, modifying its antioxidant capacity.

The primary reason for incorporating pigments into plastic materials is to impart the desired colour to finished articles. Some pigments however, may interact with the polymer leading to unexpected deleterious effects. Organic pigments, especially phthalocyanines, are favourable for their brilliant shade but are also well known for causing part distortion or warpage. This causes problems in parts which require good dimensional stability such as crates, containers, trays, caps and closures. Despite that, there are not many published studies on the root cause and mechanism of warpage induced by the pigment. Hence, the objective of this research is to study the influence of such pigments on the dimensional stability, crystallisation behaviour and morphology of polyethylenes in order to have a better understanding on the mechanism of warpage, which could possibly lead to a solution in overcoming this problem.

SOLUTION AND MELT BEHAVIOUR OF HIGH-DENSITY POLYETHYLENE - Successive Solution Fractionation mechanism - Influence of the molecular structure on the flow In the field of polyethylene characterization, one of the most challenging research topic is certainly an accurate molecular structure determination of industrial products, in terms of molar mass distribution (MMD), corresponding average-molar masses and molecular architecture (branching nature, content and heterogeneity). Solution to this long-term problem necessarily calls for a multi-disciplinary approach. Therefore, respective advantages of molecular structure characterization in solution and in the melt are exploited. In solution, chromatographic and spectroscopic methods allow determination of MMD, average branching content and intermolecular heterogeneity within their detection limits. Rheological testing in the melt could be a very powerful molecular structure investigation tool, due to its extreme sensitivity to high molar mass (MM) tailing or long chain branching (LCB) traces. But when the rheological tests results are in hand, we often still wonder what kind of molecular structure gives rise to such results. Indeed, melt signal depends on MM, MMD and LCB presence. MMD determination and LCB quantification by melt approach is impossible as long as respective effects of these molecular parameters are not clearly quantified. The general purpose of the present work is to contribute to a better molecular structure characterization of high-density polyethylene by developing, in a first time, a preparative fractionation method able to provide narrow-disperse linear and long chain branched samples, essential to separate concomitant effects of MM, MMD and LCB on rheological behaviour. Once such model fractions isolated, influence of MM and LCB on both shear and elongational flow behaviours in the melt is studied. /Dans le domaine du polyéthylène, un des sujets de recherche les plus investigués à l'heure actuelle est la détermination précise de la structure moléculaire de résines industrielles, en termes de distribution des masses molaires (MMD), de masses molaires moyennes correspondantes et d'architecture moléculaire (nature, teneur et hétérogénéité). La résolution de cette problématique nécessite une approche multi-disciplinaire, afin d' exploiter simultanément les avantages d'une caractérisation en solution et à l'état fondu. En solution, certaines méthodes chromatographiques et spectroscopiques permettent de déterminer une MMD, une teneur moyenne en branchement et leur distribution, dans leurs limites de détection. La mesure du comportement rhéologique à l'état fondu pourrait s'avérer un formidable outil de caractérisation de la structure moléculaire en raison de son extrême sensibilité à certains détails moléculaires, tels que la présence de traces de LCB ou de très hautes masses molaires (MM). Malheureusement, le signal rhéologique dépend de manière conjointe de la MM, MMD et de la présence ou non de LCB, de telle sorte que la détermination d'une MMD ou d'une teneur en LCB par cette voie est impossible aussi longtemps que les effets respectifs de ces paramètres moléculaires sur le comportement rhéologique n'ont pas été clairement et distinctement établis. L'objectif global de cette thèse est de contribuer à une meilleure caractérisation de la structure moléculaire du polyéthylène haute densité en développant, dans un premier temps, une méthode préparative de fractionnement capable de produire des échantillons, linéaires ou branchés, à MMD la plus étroite possible, indispensables en vue de séparer les effets concomitants de la MM, MMD et LCB sur le comportement rhéologique à l'état fondu. Une fois de tels objets modèles isolés, l'influence de la MM et du LCB sur le comportement rhéologique, en cisaillement et en élongation, sera étudié.

Cotxes, camions, tractors, avions... tots necessiten els neumàtics com a component imprescindible per al seu funcionament. Malauradament, degut al desgast per ús, els neumàtics tenen una vida útil limitada que obliga a renovar-los cada cert temps passant a ser Neumàtics Fora d'Ús (NFU). Paradògicament, els neumàtics són formulats per a suportar condicions extremes d'ús i, entre d'altres matèries primeres contenen cautxú vulcanitzat, una elevada quantitat d'estabilitzants i d'altres additius que fan dels neumàtics un material no biodegradable. Fins al 2006, era possible desar els NFU en abocadors però aquesta pràctica va ser prohibida per la Comunitat Europea (directiva 1993/31/EC). Aquesta directiva conjuntament amb la creixent consciència mediambiental ha potenciat la investigació per a trobar noves aplicacions per les 3.4milions de tones de NFU que es generen a l'any a Europa. Els NFU són emprats com a font d'energia o en aplicacions civils, però cada vegada més, són emprats en autopistes o per usos decoratius després de patir un procés de separació i trituració (NFU triturat). Les propietats físico-químiques dels NFU en fan un material molt interessant per la comunitat científica internacional que es centra en buscar noves vies de reciclatge mitjançant aplicacions d'alt valor afegit.Una opció per reciclar grans volums de NFU és utilitzar-los granulats com a càrrega en matrius termoplàstiques, encara que aquesta és una tasca difícil degut a la baixa compatibilitat entre aquests dos materials. Les seves característiques superficials són les responsables d'aquesta baixa compatibilitat. Cal afegir també que el tamany de les partícules de NFU obtingudes a partir de processos industrials és entre 400 and 600&#956;m, sent aquest un tamany massa gran per poder ser fàcilment embolcallades per qualsevol matriu. D'altra banda, el cautxú dels neumàtics és troba altament vulcanitzat i, per tant, aquest reaccionarà químicament amb dificultats amb d'altres materials. Totes aquestes propietats fan dels NFU un material difícil de treballar-hi. La present tesis estudia diferents mètodes de compatibilització d'un Polietilè D'alta Densitat (PEAD) de grau d'injecció i NFU triturat obtingut a partir de processos estàndards de molturació. La resina de PEAD va ser escollit com a matriu degut al seu ampli rang d'aplicacions d'injecció. L'objectiu principal és explorar diferents mètodes de compatibilització d'aquests dos materials per obtenir òptimes propietats mecàniques, tèrmiques i morfològiques en aplicacions d'injecció. Els composites són optimitzats per a que continguin la màxima quantitat de NFU, una adequada transformació i el mínim cost. Després d'un estudi preliminar de composites basats en NFU i PEAD s'han estudiat tres mètodes diferents per millorar l'adhesió entre aquests dos materials. El primer mètode consisteix en oxidar la superfície de les partícules de NFU granulat amb tractament basats en àcids, amb l'objectiu de proporcionar rugositat per a un anclatge mecànic. En el segon mètode s'han emprat additius humectants i ceres, ja que aquestes substàncies redueixen la tensió interfacial entre les partícules de NFU i la matriu de PEAD. En l'últim mètode, es va estudiar la influència de l'Etilè Propilè Diè Monòmer (EPDM). Amb aquest mètode, les partícules de NFU són embolcallades per l'EPDM, aquest efecte pot ser millorat amb l'ús de peròxids. Les propietats dels "composites" són bastant diferents en funció del mètode emprat.Després d'una comparació dels "composites" obtinguts pels tres mètodes, s'ha trobat que el que conté 30% d'EPDM i dos peròxids, corresponent a la formulació:: 30% NFU + 40% PEAD + 30% EPDM + 0.5% Trigonox 311 + 0.1% Peròxid de Dicumilcompleix els objectius fixats en la tesis: bona adhesió entre les partícules de NFU i el PEAD on l'elevat valor d'elongació n'és una conseqüència directa, la seva obtenció mitjançant un procés de compatibilització de cost reduït, un nou material amb una elevada quantitat de NFU (30%), la seva facilitat de transformació,, i el més important, una nova via de reciclatge dels NFU per a una aplicació d'alt valor afegit. / Cars, trucks, tractors, airplanes. all need tyres as essential component to work. Unfortunately, due to its continuous use, tyres suffer from wear and have a limited lifetime; therefore, they must be changed every certain time becoming end of life tyres (EOL tyres). Paradoxically tyres are formulated to withstand difficult conditions and among other raw materials, they contain vulcanizated rubbers, high amount of stabilizers and other additives that turn tyres into non biodegradable material. Until 2006, disposal of EOL tyres in landfills was a common practice but it was banned by the European Community (directive 1993/31/EC). This European legislation together with an increasing environmental consciousness has instigated researchers to find applications for 3.4million tonnes of used tyres generated per year in Europe. Some extended uses for EOL tyres are energy recover and civil applications but the use of tyres after separation and grinding processes (material know as Ground Tyre Rubber - GTR), has increased during the last decade. Physical and chemical properties of EOL tyres make them an interesting material for the international research community focused today on finding new ways to recycle tyres for value added applications. A good option to recycle big volumes of discarded tyres is using GTR particles as filler in thermoplastic matrixes, although this is a difficult task due to the low compatibility between the two materials. The surface characteristics of the GTR particles are responsible of this low compatibility. Despite these facts, the particle size obtained from standard industrial grinding processes is between 400 and 600&#956;m. These particles are too large to be entrapped easily in polymeric matrixes. These characteristics in conjunction with the fact that GTR is made of highly crosslinked rubber with difficulties to react chemically to other materials, make GTR a material very difficult to work with.The present thesis deals with the study of different compatibilization methods for an injection moulding grade of High Density Polyethylene (HDPE) and GTR obtained from standard industrial grinding process. The HDPE resin was chosen due to its wide range of injection moulding applications. The aim is to explore different methods to mix these two materials and obtain optimum mechanical, thermal and morphological properties for injection moulding applications. The composites are optimized to obtain the highest GTR amount, adequate processability and minimum cost. After a preliminary study of the composites based on GTR and HDPE, three different methods to improve the adhesion between these two materials are studied. The first method consists in an oxidizing treatment on GTR particles surface in order to promote mechanical anchoring. In the second method the influence of wetting additives and waxes is studied. These substances reduce interfacial tension between GTR particles and HDPE matrix. The third and last method, study the influence of Ethylene Propylene Diene Monomer (EPDM) addition with and without peroxides. In this method, GTR particles are encapsulated by EPDM and this effect can be boosted by the use of peroxides. The composites properties are quite different depending on the used method.After a comparison of the composites obtained by the three different compatibilization methods, it is found that the one with 30% of EPDM plus two peroxides, corresponding to30% GTR + 40% HDPE + 30% EPDM + 0.5% Trigonox 311 + 0.1% Dicumyl Peroxidefulfil the objectives of the thesis. This composite has good adhesion between GTR and HDPE that results in high elongation, it is obtained by a cheap compatibilization process and contains a high amount of recycled tyres (30%), it is easy to process, and the most important thing, it represents a new route to recycle scrap tyres for a value added application.

The monomer, p-acryloyloxybenzoic acid (ABA) was synthesized by condensation reaction of acryloyl chloride and p-hydroxybenzoic acid in alkaline medium. Polymerization of the monomer and grafting of the produced polymer (PABA) onto high density polyethylene (HDPE) were expected to be carried simultaneously in melt mixing at high temperature. The graft copolymerization was studied at varying concentrations of the monomer in the reaction mixture at constant temperature (200 0C). Grafted HDPE samples were investigated by several techniques such as DSC, FTIR, MFI and mechanical testing. The tensile tests of PABA-g-HDPE showed an improvement particularly in stress at yield and Young&rsquo / s modulus whereas the strain at break values showed a decrease for all compositions compared to neat HDPE.

The effects of polyethylene, (PE), type, compatibilizer type and organoclay type on the morphology, rheological, thermal, and mechanical properties of ternary low density polyethylene (LDPE), high density polyethylene (HDPE), and linear low density polyethylene (LLDPE), matrix nanocomposites were investigated in this study. Ethylene &ndash / Methyl acrylate &ndash / Glycidyl methacrylate terpolymer (E-MAGMA) and Ethylene &ndash / Butyl acrylate- Maleic anhydrate terpolymer (E-BA-MAH) were used as the compatibilizers. The organoclays selected for the study were Cloisite 30B and Nanofil 8. Nanocomposites were prepared by means of melt blending via co-rotating twin screw extrusion process. Extruded samples were injection molded to be used for material characterization tests. Optimum amounts of ingredients of ternary nanocomposites were determined based on to the mechanical test results of binary blends of PE/Compatibilizer and binary nanocomposites of PE/Organoclay. Based on the tensile test results, the optimum contents of compatibilizer and organoclay were determined as 5 wt % and 2 wt %, respectively. XRD and TEM analysis results indicated that intercalated and partially exfoliated structures were obtained in the ternary nanocomposites. In these nanocomposites E-MA-GMA compatibilizer produced higher d-spacing in comparison to E-BA-MAH, owing to its higher reactivity. HDPE exhibited the highest basal spacing among all the nanocomposite types with E-MA-GMA/30B system. Considering the polymer type, better dispersion was achieved in the order of LDPE&lt / LLDPE&lt / HDPE, owing to the linearity of HDPE, and short branches of LLDPE. MFI values were decreased by the addition of compatibilizer and organoclay to the matrix polymers. Compatibilizers imparted the effect of sticking the polymer blends on the walls of test apparatus, and addition of organoclay showed the filler effect and increased the viscosity. DSC analysis showed that addition of compatibilizer or organoclay did not significantly affect the melting behavior of the nanocomposites. Degree of crystallinity of polyethylene matrices decreased with organoclay addition. Nanoscale organoclays prevented the alignment of polyethylene chains and reduced the degree of crystallinity. Ternary nanocomposites had improved tensile properties. Effect of compatibilizer on property enhancement was observed in mechanical results. Tensile strength and Young&rsquo / s modulus of nanocomposites increased significantly in the presence of compatibilizers.