Alveolar macrophages (AMs) are lung tissue-resident macrophages that can be expanded in culture, but it is unknown to what extent culture affects their in vivo identity. Here we show that mouse long-term ex vivo expanded AMs (exAMs) maintained a core AM gene expression program, but showed culture adaptations related to adhesion, metabolism and proliferation. Upon transplantation into the lung, exAMs reacquired full transcriptional and epigenetic AM identity, even after several months in culture and could self-maintain long-term in the alveolar niche. Changes in open chromatin regions observed in culture were fully reversible in transplanted exAMs and resulted in a gene expression profile indistinguishable from resident AMs. Our results indicate that long-term proliferation of AMs in culture did not compromise cellular identity in vivo. The robustness of exAM identity provides new opportunities for mechanistic analysis and highlights the therapeutic potential of exAMs.

Investigação da identidade cultural na peça teatral Sinhá Moça chorou (1940-1953), do escritor sul-riograndense Ernani Fornari. Discussão da função político-ideológica do texto teatral, demonstrando a relação simbólica entre a Revolução Farroupilha (1835-1845) e o Estado Novo (1937-1945). Trata-se de uma pesquisa interdisciplinar que transita entre a Literatura, a História e a Sociologia, e que se insere no contexto dos estudos de cultura regional. / Submitted by Marcelo Teixeira (mvteixeira@ucs.br) on 2014-05-16T16:07:56Z No. of bitstreams: 1 Dissertacao Adriane A Eitelven.pdf: 598571 bytes, checksum: c2a4468123f26e703505d61836454f96 (MD5) / Made available in DSpace on 2014-05-16T16:07:56Z (GMT). No. of bitstreams: 1 Dissertacao Adriane A Eitelven.pdf: 598571 bytes, checksum: c2a4468123f26e703505d61836454f96 (MD5) / Investigation of the cultural identity in the theatrical play Sinhá Moça chorou (1940-1953) by Ernani Fornari, writer from Rio Grande do Sul. Discussion of the ideology and the political function of the theatrical text, demonstrating the symbolic relation between the Farroupilha Revolution (1835-1845) and the Estado Novo (1937-1945). Interdisciplinary discussion which covers Literature, History and Sociology, and which is inserted in the context of the studies of regional culture.

Investigação da identidade cultural na peça teatral Sinhá Moça chorou (1940-1953), do escritor sul-riograndense Ernani Fornari. Discussão da função político-ideológica do texto teatral, demonstrando a relação simbólica entre a Revolução Farroupilha (1835-1845) e o Estado Novo (1937-1945). Trata-se de uma pesquisa interdisciplinar que transita entre a Literatura, a História e a Sociologia, e que se insere no contexto dos estudos de cultura regional. / Investigation of the cultural identity in the theatrical play Sinhá Moça chorou (1940-1953) by Ernani Fornari, writer from Rio Grande do Sul. Discussion of the ideology and the political function of the theatrical text, demonstrating the symbolic relation between the Farroupilha Revolution (1835-1845) and the Estado Novo (1937-1945). Interdisciplinary discussion which covers Literature, History and Sociology, and which is inserted in the context of the studies of regional culture.

This thesis deals with an identity of young Romanies. About its development, meaning and importance when searching for oneself. To understand the significance of finding the Romanian youth´s identity, it is essential to get familiar with their culture, traditions, way of life etc. In the theoretical part it is possible to find the topic of personal development with relation to free time, its importance with creating a personality and educational methods applied to working with Romanian youth coming out of {\clq}qgood experience``. The practical part focuses on fieldwork. The research is a verification of hypotheses that were set at the beginning of the work. The next part of the research are new facts that have been found by the author during the realization of the research in fieldwork. This work is supposed to provide an educationalist a complex view of the problems connected with the identity development of the Romanian identity.