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This dissertation explores the life experiences of a particular group of young people in the Philippines known as the “istambay”, a Filipino term derived from the English phrase “on standby”. The study examines the concept of istambay and the dynamics of inactivity in the lives of selected Filipino youth. It illustrates the habitus, life contexts, and the web of crises that these youth negotiate within the protective realms of family and religion, and the dismal labour conditions in the country. Methodologically, the study utilizes a mixed methods approach that includes both qualitative and quantitative aspects. Primary qualitative data are derived from case studies, songs, field notes, and ethnographic observations. Secondary quantitative analyses are conducted using the Young Adult Fertility and Sexuality Study (YAFS) 2002 and a perceptual survey. The study is theoretically oriented around Mills’ sociological imagination and Bourdieu’s theory of practice. It illustrates that the istambay phenomenon is more than a personal trouble (problematic istambay). It is a public issue (istambay problematique). The study also shows the varying levels of vulnerability experienced by different types of istambay. Research findings reveal the istambay phenomenon is a manifestation of the interrelated problems in the educational system and the labour market of the Philippines. The consequence of inactivity among these istambays is found to be mitigated by the culture of care provided by Filipino families combined with solace derived from the church. However, istambays are also found to express disconnection from the state because of the government’s inability to provide employment for them, resulting in their quest for employment “abroad” (overseas) as an alternative. Youth inactivity, although moderated by a familial-faith dynamic, highlights how complex forms of inequalities in the Philippines marginalize the istambays from gaining legitimate active status in Philippine society.

Mutations in RHBDF2, the gene encoding inactive rhomboid protein iRHOM2, result in the dominantly inherited condition Tylosis with oesophageal cancer (TOC). TOC causes plamoplantar keratoderma, oral precursor lesions and up to a 95 % life-time risk of oesophageal squamous cell carcinoma (SCC). The role of iRHOM2 in the epidermis is not well characterised, although we previously showed dysregulated epidermal growth factor receptor (EGFR) signalling and accelerated migration in TOC keratinocytes, and a role for iRHOM2 was shown in trafficking the metalloproteinase ADAM17. Substrates of ADAM17 include EGFR ligands and adhesion molecules. iRHOM2 localisation and function were investigated in frozen sections and keratinocyte cell lines from control and TOC epidermis. Although iRHOM2 was predicted to be an ER-membrane protein, it showed cell-surface expression in control epidermis, with variable localisation in TOC. Increased processing and activation of ADAM17 was seen in TOC keratinocytes compared with control cells, suggesting that increased ADAM17-mediated processing of EGFR ligands may cause the changes in EGFR signalling. Downstream of iRHOM2-ADAM17, Eph/Ephrin and NOTCH signalling also appeared affected. Additionally, desmosomes in TOC epidermis lacked the electron-dense midline of the mature desmosomes seen in normal skin; this was accompanied by increased processing of desmoglein 2, a substrate of ADAM17. Expression and localisation of iRHOM2 was also investigated in TOC and sporadic SCC. iRHOM2 expression varied between SCC cell lines, and appeared to correlate with ADAM17 and NOTCH1 expression in oesophageal SCC and head and neck SCC cells. In summary, iRHOM2 mutations in TOC appear to be gain-of-function in nature, resulting in increased ADAM17 processing and enhanced EGFR signalling. Questions remaining include the reason why iRHOM2 is found at the plasma membrane. Future study of the iRHOM2-ADAM17 pathway may provide additional insight into the mechanism of epidermal wound healing and the pathogenesis of oesophageal SCC.

This thesis is a study dealing with active and inactive L.D.S. couples and their comparative Marital adjustment. The couples were selected from the geographic areas of Enterprise and Logan, Utah, and Preston, Idaho. Respondents consisted of couples who had at least one child, but no children beyond high school age. Their religiosity was determined by the bishop of the L.D.S. Ward of which they were a member. The bishop considered such factors as attendance at regularly scheduled church meetings and contributions made in the form of tithes in determining whether a couple was considered active or inactive. The sample consisted of 40 active couples and 20 inactive couples. Hypotheses tested in this study were (1) L.D.S. couples who are active in church activities have a higher degree of marital adjustment than those couples who are inactive. (2) L.D.S. couples who are active will rate their marriages as happier on the continuum scale of happiness than will couples who are inactive. (3) Church activity of the part of both husband and wife contributes to the rapport and marital success of the couple. (4) Couples who are least active will be less likely to participate in the study; therefore their marriage adjustment may not be discovered. In testing the hypotheses subjects were given a modified marital adjustment inventory used by Locke, plus questions pertaining to church activity and a set of background questions. The questionnaires were given directly to the subjects by the investigator or were sent by mail to subjects with a letter of instructions. Findings of this study appeared to indicate that active couples had a better marital adjustment. Adjustment scores for active couples ranged from a low of 71 to a high of 123 with an arithmetic mean of 107.80. Adjustment scores for inactive couples ranged from a low of 60 to a high of 121 with an arithmetic mean of 99.15. A "t" ratio of 3.86 was obtained, which is significant at the level of .01 level of significance. It is to be noted that when adjustment scores of active husbands were compared with inactive, the difference was not so significant. It only approached significance at the .05 level of significance; still active husbands had somewhat higher adjustment scores the arithmetic mean of active husbands was 108.45 compared with 100.95 which was the arithmetic mean for inactive husbands. The difference between active and inactive wives was more significant. Active wives had an arithmetic mean of 107.15 as compared with 97.35 for the inactive. This difference approached significance at the .01 level of significance. A conclusion of the study is, church activity appears to be a factor contributing to the happiness of the couples and to the adjustments of their marriages.

Orientador: Helia Harumi Sato / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-01T14:14:34Z (GMT). No. of bitstreams: 1 Ferro_LilianAparecida_D.pdf: 39343182 bytes, checksum: 85dd4ff7fee79e9940cf96d45fae535d (MD5) Previous issue date: 2002 / Resumo: Este trabalho objetivou o isolamento de microrganismos capazes de produzir enzimas que lisam a parede celular de leveduras, a produção, purificação e caracterização de 13-1,3-glucanase lítica. As bactérias líticas foram isoladas de Iodo da usina de açúcar e álcool Santa Helena, Piracicaba, SP. As bactérias líticas isoladas aderiram-se às células viáveis de Saccharomyces cerevisiae 701 e lisaram-nas. A linhagem YLM-B191-1, selecionada para o estudo, foi identificada através das características bioquímicas e fisiológicas como Cellulomonas cellulans. Para a produção da 13-1,3-glucanase lítica, a linhagem de C. cellulans YLM-B191-1 foi cultivada em meio composto de 15 9 de levedura seca; 2,0 9 de (NH4hSO4; 13,6 9 de KH2PO4; 4,2 9 de KOH; 0,2 9 de MgSO47H2O; 0,001 9 de Fe2(SO4h 6H2O; 1 mg de biotina e 1 mg de tiamina por litro. A 13-1,3-glucanase foi purificada do sobrenadante do meio de cultura através de ultrafiltração e cromatografia em coluna de CM-Sepharose CL-6B. A enzima purificada apresentou atividade ótima a 55°C e na faixa de pH entre 4,5 a 6,5. A 13-1,3- glucanase purificada apresentou estabilidade na faixa de pH 5,5 a 6,5 e foi inativada em temperaturas superiores a 55°C. A massa molecular da 13-1,3- glucanase purificada foi estimada em 17,1 kDa através de SDS-PAGE. A 13-1,3- glucanase purificada hidrolisou as ligações 13-1,3-glicosídicas da laminarina atuando como uma endoenzima. Através de microscopia eletrônica de varredura, observou- se que as enzimas líticas da linhagem de C. cellulans YLM-B191-1 foram capazes de alterar a superfície celular de leveduras / Abstract: The objective of this research was to isolate microorganisms which produced yeast cell walllytic enzymes and to study the production, purification and characterization of a Iytic ~-1,3-glucanase. The yeast-Iytic bacterium was isolated from the sludge of the Santa Helena sugar and alcohol factory in Piracicaba, SP. The isolated yeast-Iytic bacterium adhered to viable cells of Saccharomyces cerevisiae701 and Iysed them.The yeast-IyticbacteriumYLM-8191-1, selected for this study, was identified as Cellulomonas cellulans, from its biochemical and physiological characteristics.The strain C. cellulansYLM 8191-1 was cultivated in a medium containing (per liter) 2.0 9 of (NH4hSO4; 13.6 9 of KH2PO4;4.2 9 of KOH; 0.2 9 of MgSO47H2O; 0.001 9 of Fe2(SO4h6H2Oand 1 mg each of biotin and thiamin being supplemented with 15 9 of dried yeast as the carbon source for the production of ~-1,3-glucanase. The ~1 ,3-glucanase was purified from the culture fluid of C. cellulans YLM-8191-1 by ultrafiltration and CM-Sepharose CL-68 column chromatography. The purified enzyme showed greatest activity at 55°C and between pH 4.5 - 6.5. The purified ~-1,3-glucanase was stable in the range from pH 5.5 to 6.5 and was inactivated by heating at temperatures above 55°C. The molecular weight of purified ~-1,3-glucanasewas estimated at about 17.1 kDa by SDS-PAGE. The ~-1,3-glucanase hydrolyses the ~-1,3-glucosidic linkages of the laminarin acting as an endoenzyme. Scanning electron microscopy showed that Iytic enzymes from C. cellulans YLM-8191-1 were able to modify the cellular surface of yeast / Doutorado / Doutor em Ciência de Alimentos

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The present study was conducted to determine the effect of a possible selves intervention on self-regulatory efficacy and exercise behaviour. Participants were 19 men and 61 women (Mage= 21.43, SD = 3.28) who reported exercising less than 3 times per week. Participants were randomly assigned to a control condition, a hoped-for possible selves intervention condition, or a feared possible selves intervention condition. Participants in the hoped-for and feared possible selves conditions completed an activity where they imagined themselves in the future as either healthy, regular exercisers or unhealthy, inactive individuals, respectively. Participants in the control group completed a quiz about physical activity. Measures of self-regulatory efficacy (scheduling, planning, goal-setting, and barrier self-efficacy) were taken immediately before and after exposure to the intervention. Participants who received a possible selves intervention reported greater exercise behaviour 4 weeks post-intervention than participants in the control group (p = .05). Furthermore, planning self-efficacy was found to partially mediate the effect of the possible selves intervention on exercise behaviour. These findings suggest that possible selves may play a role in increasing exercise behaviour among inactive individuals. Future research is warranted to examine the role of possible selves interventions in increasing exercise behaviour and to determine which other variables may mediate this intervention-exercise behaviour relationship. / Thesis / Master of Science (MSc)

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On pense souvent que notre mémoire nous joue des tours et nous fait défaut. Ces oublis concernent généralement des souvenirs qui ne sont pourtant pas si vieux. Alors quand il s’agit de se rappeler d’informations sensorielles auxquelles nous n’avons pas repensé depuis plusieurs dizaines d’années, pas de doute, il ne reste plus rien… Mais en est-on vraiment sûr? Et si l’information était toujours là, prête à se manifester de manière explicite -comme sous forme de rappel ou de sentiment de familiarité- pour peu que les conditions le lui permettent? A ce jour, très peu d’expériences ont été menées sur ce sujet. L’objectif de ma thèse a donc été d’apporter des premiers éléments de réponse concernant la récupération de mémoires laissées inactives. Ma recherche s’est orientée selon deux axes principaux : une première partie théorique où je montre a) qu’il n’est pas nécessaire de réactiver une trace mnésique pour la maintenir à très long-terme si elle a été suffisamment répétée et b) que la récupération explicite de cette mémoire serait possible grâce à la présentation d’informations qui cibleraient au mieux le souvenir ; une seconde partie expérimentale où je montre a) que des participants sont capables de récupérer de manière explicite des informations laissées en dormance pendant au moins une dizaine d’années et b) que cette trace mnésique pourrait être détectée sur des tracés d’Electroencéphalographie (EEG). Ainsi, et aussi étonnant que cela puisse paraître, dans des conditions favorables à leur réactivation, d’anciennes traces mnésiques que l’on croyait disparues, peuvent de nouveau surgir sous la manifestation de rappel ou de sentiment de familiarité par exemple. Ces résultats soulèvent des questions majeures concernant le stockage neuronal de cette information. / Memory plays tricks on us and fails us even for recent events. So for the retrieval of sensory information that we have not experienced for decades, surely the memories are gone without a trace, but can we be sure of this? What if the information had been there all along ready to be explicitly retrieved through recall or familiarity for example? So far, experimental evidence is lacking. The purpose of my thesis was to shed some light on the retrieval of these inactive memories. To tackle this problem I developed my research around two main axes: For the first part which is theoretical, I suggest that a) reactivations are not necessary to maintain very long-term memories if the memories were sufficiently repeated at first; b) it might be possible to retrieve explicitly remote inactive memories using specific cues; in the second part which is experimental, I show that a) within specific conditions participants are able to retrieve explicitly very long-term memories that were left inactive for decades and b) such memory traces could be found on the Electroencephalography (EEG) signals. Surprisingly, when conditions are met, remote memories that were thought to be lost can again elicit recall or familiarity. With these results, a question remains: How do neurons store such information?

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal