Dentre as diversas questões envolvendo a tecnologia duplo haploides (DH) em milho, uma que tem sido pouco discutida é a geração em que se deve induzir haploides, no caso, F1 ou F2. Destas, a F1 tem sido a mais utilizada. No entanto, o seu uso constante pode levar a perdas de ganhos genéticos, devido ao menor número de recombinações. Com isso, alguns autores aconselham o uso da F2 na indução, o que possibilitaria maiores ganhos em variabilidade genética. Desse modo, os objetivos foram verificar o efeito das gerações F1 e F2 na expressão do R1-navajo em germoplasma tropical, na eficiência relativa de cada uma das etapas da metodologia e na variabilidade genética das linhagens DH obtidas. Para isso, cinco fontes de germoplasma, em gerações F1 e F2, foram cruzadas com o indutor de haploidia tropicalizado LI-ESALQ. As sementes deste cruzamento foram agrupadas por meio do marcador R1-navajo em três classes: haploides putativos, diploides e inibidas. Após esta etapa, as sementes dos haploides putativos foram submetidas à duplicação cromossômica e as plantas duplicadas foram transplantadas a campo para a obtenção de linhagens DH. As unidades de sementes, plântulas e plantas em cada etapa da metodologia foram quantificadas para o estudo da eficiência relativa na obtenção de DH. Também foram coletadas amostras foliares das linhagens DH para genotipagem por meio de marcadores SNP (Single nucleotide polymorphism). As taxas de indução de haploides (HIR), sementes diploides (DSR) e inibidas (ISR) foram analisadas por meio de um modelo linear generalizado misto considerando distribuição logit multinomial. As eficiências relativas das fontes de germoplasma e gerações em cada etapa da metodologia DH foram estimadas por meio de porcentagem. Os marcadores SNP foram utilizados em estudos de diversidade genética, estrutura populacional e desequilíbrio de ligação. Os valores médios observados para as taxas de HIR, ISR e DSR foram, respectivamente, 1,23%, 23,4% e 75,2% para a geração F1 e 1,78%, 19,6% e 82,3% para a geração F2. O maior valor de HIR na F2 ocorreu devido à segregação dos genes que inibem o marcador R1-navajo durante a indução de haploides. Entretanto, apesar da geração F2 apresentar maior HIR, ela não deve substituir a F1, uma vez que se perde tempo com um ciclo adicional. A eficiência relativa na obtenção de linhagens DH apresentou o mesmo valor (0,4%) para as gerações F1 e F2, indicando que a escolha da geração não interfere na quantidade de DH produzidos. As estimativas dos parâmetros populacionais para as linhagens DH obtidas de geração F1 apresentaram valores para variância genética (VG) de 700,55, tamanho efetivo populacional (Ne) de 43,1, diversidade genética de Nei (GD) de 0,28 e conteúdo de informação polimórfica (PIC) de 0,23. Para a geração F2 as estimativas foram de VG=648,88, Ne=39,61, GD=0,26 e PIC=0,22. Os valores de desequilíbrio de ligação foram de 0,069 na geração F1 e de 0,067 na geração F2. Ou seja, as linhagens DH oriundas destas duas gerações apresentaram magnitudes semelhantes de diversidade genética e de desequilíbrio de ligação. O uso da geração F2 teoricamente permitiria obter maior variabilidade genética, devido à recombinação adicional. Entretanto, neste trabalho esta tendência não foi observada. Com isto, em milho tropical, recomenda-se o uso da geração F1 para a obtenção de DH, por apresentar o melhor balanço entre tempo e variabilidade genética. / Among the several questions involving doubled haploid technology (DH), one that has been little discussed is the generation in which one should induce haploids, in the F1 or F2. Overall, the F1 generation has been the most used. However, their constant use can lead to losses of genetic gains with the selection cycles due to the lower number of recombination. Thereby, some authors advise the use of F2 in inductions, which would allow greater gains in genetic variability. Thus, the objectives were to check the effect of the F1 and F2 generations on the expression of the R1-navajo in tropical germplasm, on the relative efficiency of each step of the methodology and the genetic variability of the DH lines obtained. For this purpose, five germplasm sources, in F1 and F2 generations, were crossed with the tropicalized haploid inducer LI-ESALQ. The seeds from this cross were grouped using the R1-navajo marker into three classes: putative haploids, diploid and inhibited. Then, putative haploid seeds were submitted to chromosome duplication, and the duplicate seedlings were transplanted to the field in order to obtain DH lines. Hence, seed, seedling, and plant at each stage of the methodology were quantified to study the relative efficiency in developing DH lines. Moreover, leaf samples from the D0 lines were collected for genotyping using SNP (Single nucleotide polymorphism) markers. Finally, the haploid inducer rate (HIR), diploid seed rate (DSR) and inhibited seed rate (ISR) were analyzed using a generalized linear mixed model considering multinomial logit distribution. The relative efficiency of germplasm sources and generation in each stage of the DH methodology was estimated by percentage. SNP markers were used in studies of genetic diversity, population structure, and linkage disequilibrium. The observed mean values of HIR, ISR and DSR were, respectively, 1.23%, 23.4% and 75.2% for the F1 generation and 1.78%, 19.6% and 82.3% for the F2 generation. The higher value of HIR in F2 occurred due to the segregation of genes, which inhibit the R1-navajo marker during haploid induction. However, in spite of the higher value of HIR for F2 generation, it should not replace F1 since time is lost with an additional cycle. The relative efficiency observed in the obtention of DH lines was the same value (0.4%) for generations F1 and F2, indicating that the choice between those generations does not interfere with the quantity of produced DH. Estimates of the population parameters for the DH lines obtained from F1 generation presented values for genetic variance (VG) of 700.55, effective population size (Ne) of 43.1, Nei\'s genetic diversity (DG) of 0.28 and polymorphic information content (PIC) of 0.23. For F2 generation the estimates were VG = 648.88, Ne = 39.61, GD = 0.26 and PIC = 0.22. Linkage disequilibrium values were 0.069 in the F1 generation and 0.067 in the F2 generation. Thus, DH lines from these two generations showed similar values of genetic diversity and linkage disequilibrium. Nonetheless, the use of the F2 generation theoretically would allow obtaining greater genetic variability, due to the additional cycle of crossing-overs. However, this trend was not observed in this study. Thus, in tropical maize, the use of the F1 generation to obtain DH lines is recommended, because it performs the best balance between time and genetic variability.

Submitted by Mario BC (mario@bc.ufrpe.br) on 2017-03-16T14:18:00Z No. of bitstreams: 1 Ariele Carneiro de Andrade.pdf: 1066650 bytes, checksum: 26d95d8f20aec5260de9adb89d2195cd (MD5) / Made available in DSpace on 2017-03-16T14:18:00Z (GMT). No. of bitstreams: 1 Ariele Carneiro de Andrade.pdf: 1066650 bytes, checksum: 26d95d8f20aec5260de9adb89d2195cd (MD5) Previous issue date: 2015-07-15 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The bell pepper (Capsicum annum L.) crop is one of the most important vegetables grown in Brazil, and has been showing significant growth. However, this culture can be affected by a number of diseases that cause economic damage and anthracnose, still represent a serious obstacle to production, both in the field and at home-de-vegetation. Anthracnose in Capsicum annum can be caused by several species of fungi of the genus Colletotrichum, but in Brazil, the majority of reports identify Colletotrichum gloeosporioides as the main causative agent of the disease. Infections characterized in lesions aerial parts of the plants such fruits, stems and leaves. The control method of the infections is used in fungicidal applications, however currently search for more sustainable alternatives. Thus the use of resistance inducers has been outstanding and can be applied preventively, by acting on the physiological and biochemical processes of plants. Given the above, the objective of this study was to investigate the efficacy of acibenzolar-S-methyl inducer for the control of anthracnose in pepper plants as well as the activation of enzymes related to pathogenicity and they peroxidase, polifeniloxidase, catalase, ascorbate peroxidase and β-1,3-glucanase.. The study was conducted in a greenhouse in Garanhuns-PE municipality, and the treatments were arranged in subdivided portion, in which the portion consisted of collection times and the subplot of different inducer of doses, with four replications. The plants were treated with the treatments as follows: 0.15g L-1; 0.30g L-1; 0.45g L-1, 0.60g L-1 acibenzolar-S-methyl (Syngenta®) and control (distilled water only). The results were analyzed by the software program SAEG 5.0 to 5% probability. The peroxidase activity was not significant for analysis of variance at 5% probability, however the dose 0.45g L-1 promoted greater activity. For peroxidase although not significant ascorbate activity to analysis of variance, the dose 0.15g L-1 showed increased activity of this enzyme is superior to the control. The increase in catalase activity was significant when the plants were treated with the highest dose of 0.60g L-1 inducer. On the eighth day after inoculation was higher by polyphenol oxidase activity, standing out from the others collection times. The dose 0.60g L -1 promoted greater activity of β-1,3-glucanase enzyme. When the plants medium subjected to the application of acibenzolar-S-methyl there was a reduction in the severity as the doses increased, thereby showing that prior treatment with resistance inducer consists of an efficient method for control of anthracnose disease. / A cultura do pimentão (Capsicum annum L.) é uma das mais importantes hortaliças cultivadas no Brasil. Entretanto essa cultura pode ser acometida por uma série de doenças que acarretam danos econômicos e a antracnose, ainda representa um sério entrave à produção, tanto em campo quanto em casa-de-vegetação. A antracnose nesta olerícola pode ser causada por várias espécies de fungos do gênero Colletotrichum, mas no Brasil, a maioria dos relatos identificam Colletotrichum gloeosporioides como o principal agente causal da doença. As infecções se caracterizam por lesões nas partes aéreas das plantas como nos frutos, caules e folhas. O método de controle das infecções mais utilizado consiste em aplicações de fungicidas, no entanto atualmente se busca por alternativas mais sustentáveis. Desse modo o uso de indutores de resistência vem se destacando podendo ser aplicado de forma preventiva, por atuar nos processos fisiológicos e bioquímicos das plantas. Diante do exposto, o objetivo deste estudo foi verificar a eficácia do indutor acibenzolar-S-metil para o controle da antracnose nas plantas de pimentão bem como a ativação de enzimas relacionadas à patogenicidade sendo elas peroxidase, polifeniloxidase, catalase, ascorbato peroxidase e β-glucanase. O estudo foi conduzido em casa de vegetação no município de Garanhuns-PE. Os tratamentos foram dispostos em parcela subdivida, na qual a parcela consistiu das épocas de coleta e a subparcela das diferentes doses do indutor, com quatros repetições. As plantas receberam os tratamentos da seguinte forma: 0,15g L-1, 0,30g L-1, 0,45g L-1, 0,60g L-1 do acibenzolar-S-metil (Syngenta®) e Controle (somente água destilada). Os resultados obtidos foram analisados pelo emprego do programa software SAEG 5.0 a 5% de probabilidade. A atividade peroxidase não foi significativa para análise de variância a 5% de probabilidade, no entanto a dose 0,45g L-1 promoveu maior atividade. Para a ascorbato peroxidase embora não significativo para análise de variância, a dose 0,15g L-1 apresentou maior atividade desta enzima sendo superior ao controle. O aumento na atividade catalase foi significativo quando as plantas foram tratadas com a maior dose do indutor 0,60g L-1. No oitavo dia após a inoculação houve maior atividade da enzima polifenoloxidase, destacando-se das demais épocas de coletas. A dose 0,60g L-1 promoveu maior atividade da β-1,3-glucanase. Quando as plantas foram submetidas à aplicação do acibenzolar-S-metil verificou-se uma redução da severidade à medida que as doses aumentavam, mostrando com isso que o tratamento prévio com indutor de resistência consiste em um método eficiente para o controle da doença antracnose.

One of the large lineages of parasitic wasps, the Cynipoidea, exhibits three distinctly different life modes. Slightly more than half of the about 3000 species are parasitoids in insect larvae, whereas the remaining species are associated with plants, either as gall inducers or as inquilines (guests feeding on plant tissue in galls). The main focus of this thesis has been to identify morphological changes associated with the shifts between life modes. Particular attention was paid to structures believed to be important in gall initiation. Comparative anatomical studies of the egg, larva and venom apparatus were performed, including representatives of parasitoids, gall inducers and inquilines. Examination of gross morphology and ultrastructure revealed that the eggs of the gall inducers are larger and surrounded by a thicker shell than the parasitoid eggs. These differences may be related to the fact that the gall inducer egg contains sufficient egg yolk for the embryo during the entire egg period, whereas the parasitoid egg often absorbs nutrients through the eggshell. Furthermore, the gall inducer egg is probably more exposed to desiccation and therefore a thicker and more resistant eggshell is crucial. Comparing the terminal-instar larvae of about 30 species of parasitoids, gall inducers and inquilines, extensive morphological variation was found, particularly in the head and mouthpart features. The variation was summarized in 33 morphological and one life-history character and parsimony analyses were performed. The resulting phylogenetic estimates were largely in accordance with previous analyses of adult morphology and molecular data. The larval data point to a single origin of the inquilines, in agreement with adult morphology but in conflict with molecular data. The venom apparatus was found to be quite uniform in structure among a sample of 25 species of cynipoid species. It consists of a very short venom duct, a reservoir and a single unbranched venom gland. With few exceptions, the venom apparatus is conspicuously larger relative to the female metasoma in the gall inhabiting species than in the parasitoids. We found little evidence of anatomical structures that could facilitate chemical communication between the gall-inducer embryo and the surrounding plant tissue through the thick eggshell. On the other hand, the enormous venom glands and reservoirs, which are apparently not used for defence, suggest that the adult female plays a significant role in gall induction by injecting secretions into the host plant when laying eggs.

This work presents design and fabrication technologies on vibration-induced electromagnetic micro-generators using LTCC (Low temperature co-fire ceramic) processes. LTCC fabrication with some special advantages has simplistically processes and multilayer stack procedure, resulting in a micro-inducer can consist of the multilayer silver (Ag) induction micro-coils and a helical ceramic micro-spring. Highly electrical conductible Ag and its multilayer micro-coil structures can enhance the power output of generators. This work is composed of three parts. The first part describes the design of two kinds of micro-generator; a magnetic core generator (MCG) and sided-magnet generator (SMG). According to their respective structures, an analytical mode is also developed to investigate its resonant frequency and the spring constant of the micro-spring, as well as the bending stress and fatigue life of the supporting beam. The voltage output, current output and power output on the helical induction micro-coils, as well as the relationship of vibration amplitude versus vibration frequency in the vibrating system are calculated. The second part introduces how to integrate the Ag multilayer induction micro-coils and the helical ceramic micro-spring using LTCC technique, and organize the design and fabrication of LTCC micro-inducers. From the fabrication procedures, it is known that a stacking error places a limit on the total numbers of micro-coils layer. The experimental results verify that the application of LTCC to the fabrication of micro-inducers is feasible, and that the phenomenon of plane warpage, volumetric shrinkage, layer delamination and surface crack of sintered ceramic structures has been fully controlled. In the third part, measurement setup, vibrating tests and experiments on generating electricity are completed. The performances with different-structure devices are evaluated. Voltage output, current output and power output, as well as changing trends of power density with respect to the layer number of induction micro-coils and magnets are discussed. Relationship of the electrical parasitical damping coefficient versus the vibration amplitude and vibration velocity, relationship between the induced inductor and the current output, the power output depending on the electrical load resistance and differences between fabrication lots are investigated. At last, comparisons between analytical and experimental power output are conduced. For MCG micro-generator, the analytical value is 0.88 mW, about 13.6% smaller than the experimental value of 1 mW. For SMG micro-generator, the analytical value is 1.73 mW, about 10.7% larger than the measured value of 1.56 mW. The analytical models are verified. In the MCG device, the experimental results show that a maximum voltage output of 25.19 mV, a current output of 82.9 mA and a power density of 2.36 mW/cm3 under 120 Hz frequency and 0.03-mm amplitude are obtained. In addition, when operated at 69 Hz vibration frequency and vibration amplitude of 0.03 mm, the experimental maximum voltage output, current output and power density of the SMG device are 44.5 mV, 83.1 mA and 2.17 mW/cm3, respectively. Except the power density, other electricity performances of SMG device are better than MCG. Apparently, the power density of MCG and SMG device presented by this study competes favorably with the results from other devices in the literature.

A panel method derived from inviscid irrotational flow theory and utilizing hyperboloid panels is developed and applied to the simulation of steady fully wetted flows inside water-jet pumps and rocket engine inducers. The source and dipole influence coefficients of the hyperboloid panels are computed using Gauss quadrature. The present method solves the boundary value problem subject to a uniform inflow directly by discretizing the blade, casing/shroud and hub geometries with panels. The Green's integral equation and the influence coefficients for the water-jet/inducer problem are defined and solved by allocating constant strength sources and dipoles on the blade, hub and casing surfaces and constant strength dipoles on the shed wake sheets from the rotor/ stator blades. The rotor- stator interaction is accomplished using an iterative procedure which considers the effects between the rotor and the stator, via circumferentially averaged induced velocities. Finally, the hydrodynamic performance predictions for the water-jet pump and the inducer from the present method are validated against existing experimental data and numerical results from Reynolds Averaged Navier- Stokes (RANS) solvers. / text

The Bar-Or laboratory recently reported that human B cells from normal subjects can produce either pro-inflammatory (TNF-alpha; LT) or regulatory (IL-10) effector cytokines depending on their context of activation. It was of interest to investigate the change in B cell cytokine profiles from normal subjects when activated in the context of a Th1 pro-inflammatory environment or a Th2 anti-inflammatory environment. It was found that the B cell regulatory network of effector cytokines from normal subjects is significantly modulated depending on the local cytokine milieu. In addition, it was of interest to study how MS patients' B cell cytokine network would respond in a Th1 pro-inflammatory and a Th2 anti-inflammatory context. It was found that MS patients' B cell cytokine network was dysregulated compared to B cell responses from normal subjects. The findings define a novel regulatory network involving human B cells from normal subjects and point to a newly discovered abnormality in MS patients' B cells.

Thesis (Ph. D.)--West Virginia University, 2005 / Title from document title page. Document formatted into pages; contains ix, 155 p. : ill. Vita. Includes abstract. Includes bibliographical references.

Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 64-80).

Submitted by Socorro Pontes (socorrop@ufersa.edu.br) on 2016-11-21T14:28:49Z No. of bitstreams: 1 BeatrizLSC_DISSERT.pdf: 636701 bytes, checksum: eb29301209fc73534418e41fd55533a4 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-03-21T14:37:55Z (GMT) No. of bitstreams: 1 BeatrizLSC_DISSERT.pdf: 636701 bytes, checksum: eb29301209fc73534418e41fd55533a4 (MD5) / Approved for entry into archive by Vanessa Christiane (referencia@ufersa.edu.br) on 2017-03-21T15:04:24Z (GMT) No. of bitstreams: 1 BeatrizLSC_DISSERT.pdf: 636701 bytes, checksum: eb29301209fc73534418e41fd55533a4 (MD5) / Made available in DSpace on 2017-03-21T15:04:33Z (GMT). No. of bitstreams: 1 BeatrizLSC_DISSERT.pdf: 636701 bytes, checksum: eb29301209fc73534418e41fd55533a4 (MD5) Previous issue date: 2016-02-26 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / The melon is a crop of great economic importance to Brazil and especially in the Northeast region, where production is mainly for the foreign market. Phytosanitary problems of culture, however, are numerous and have increased the need to use pesticides, considering that the ones allowed for this culture are increasingly scarce in the country and foreign market. The use of alternatives to improve the plant health and fruit quality is welcome and essential to a more sustainable agriculture and less damaging to the environment. This study aimed to evaluate the effect of calcium silicate in the management of leafminer and powdery mildew melon crop and quality of post-harvest fruits. The experiment was conducted at Agrícola Famosa farm, located in the municipality of Baraúna, Rio Grande do Norte, Brazil. In the first stage of experiment, we evaluated the effect of calcium silicate in the plant of melon and in the second, we evaluated physical and chemical qualities of postharvest melon. For phytosanitary analysis, we were assessed the severity of powdery mildew and the number of leafminer mines in the area that received and not calcium silicate, using a completely randomized design and 26 repetitions. The evaluations were performed at 7, 14, 21, 28 and 35 days after removal of TNT manta. At the end of the cycle, in order to analyze the quality of post-harvest fruit, 75 fruit each area with and without calcium silicate were picked at random and evaluated for physical and chemical characteristics in 5 storage periods (0, 21, 28, 35 and 42 days) and 5 replicates, with 3 fruits per repetition. The experimental design was completely randomized. Calcium silicate reduced the severity of powdery mildew and leaf lesions caused by leafminer and provided firmer, less mass loss and pulp thickness and did not alter the chemical characteristics such as pH, total soluble solids and titratable acidity of fruits post-harvest / O melão é uma olerícola de grande expressão econômica para o Brasil e em especial para a região Nordeste, onde a produção é principalmente destinada ao mercado externo. Os problemas fitossanitários desta cultura, no entanto, são inúmeros e têm aumentado a necessidade do uso de defensivos, sendo os permitidos para esta cultura cada vez mais escassos no país e no exterior. O uso de alternativas que melhorem as condições fitossanitárias e a qualidade dos frutos é bem-vindo e essencial para uma agricultura mais sustentável e com menores danos ao ambiente. O presente estudo teve como objetivo avaliar o efeito do silicato de cálcio no manejo da mosca minadora e do oídio do meloeiro e na qualidade dos frutos pós-colheita. O experimento foi conduzido na fazenda Agrícola Famosa, localizada no município de Baraúna, Rio Grande do Norte, Brasil. Na primeira etapa do experimento, avaliou-se o efeito do silicato de cálcio na fitossanidade do meloeiro e na segunda, as qualidades físicas e químicas pós-colheita do melão. Para as análises fitossanitárias, foram avaliados a severidade do oídio e o número de minas de mosca minadora na área que recebeu e não o silicato de cálcio, utilizando-se o delineamento experimental inteiramente casualizado e 26 repetições. As avaliações foram realizadas aos 7, 14, 21, 28 e 35 dias após a retirada da manta de TNT. Ao final do ciclo da cultura, para analisar a qualidade dos frutos pós-colheita, 75 frutos de cada área com e sem silicato de cálcio foram colhidos ao acaso e avaliados quanto às características físicas e químicas em 5 períodos de armazenamento (0, 21, 28, 35 e 42 dias) e 5 repetições, com 3 frutos por repetição. O delineamento experimental utilizado foi inteiramente casualizado. O silicato de cálcio diminuiu a severidade de oídio e as lesões foliares causadas por minadoras e proporcionou maior firmeza, menor perda de massa e espessura de polpa e não alterou as características químicas como o pH, sólidos solúveis totais e acidez total titulável dos frutos pós-colheita / 2016-11-21

Submitted by Mario BC (mario@bc.ufrpe.br) on 2016-06-14T16:02:16Z No. of bitstreams: 1 Victor Netto Maia.pdf: 954749 bytes, checksum: c7a0fb4acc16853fce85c1219537a372 (MD5) / Made available in DSpace on 2016-06-14T16:02:16Z (GMT). No. of bitstreams: 1 Victor Netto Maia.pdf: 954749 bytes, checksum: c7a0fb4acc16853fce85c1219537a372 (MD5) Previous issue date: 2015-03-31 / Much has been made in order to enhance the implementation and improve results of assisted reproductive biotechnologies in mares, however there are still few studies on vascularization of the corpus luteum (CL) and its relation to progesterone and angiogenic factors and their consequences when the ovulation is induced with deslorelin acetate. Mares were used in natural ovulation (GC) condition and induced (GT) with deslorelin (>35 mm follicles). In the experiment 1 ovulation and CL formation were followed by Doppler ultrasound (6.5 MHz), on days 4, 8 and 12 after ovulation (D0). Blood samples were obtained for progesterone assay by enzyme-immunoassay. The CLs were analyzed for vascularization, and images were recorded in video. Image analysis considered the percentage of luteal parenchymal area presenting color signals. The results were presented as mean ± epm. The average vascularization was in GC: 76.25 ± 8.98; 70.00 ± 8.16 and 57.50 ± 13.31 % and in GT: 85.00 ± 5.40, 90.00 ± 0.00 and 58.75 ± 9.24 % respectively for the days 4, 8 and 12 after ovulation. Progesterone concentrations were in GC: 6.21 ± 0.61; 5.82 ± 1.44 and 4.63 ± 0.79 ng/mL and GT: 6.10 ± 1.18; 4.67 ± 1.06 and 4.49 ± 1.37 ng/ml, respectively for the 4, 8 and 12 days after ovulation. The patterns of vascularization were higher in D4 and D8 compared to D12 of GT (P<0.05). However, there were no differences between groups or days in GC. The concentration of progesterone didn’t show significant differences between groups or studied days. In experiment 2, samples of CL were collected through ultrasound guided biopsy at the same time points after ovulation. The mRNA expression of VEGF, BFGF and LHR genes were analyzed by real time PCR. It was observed positive correlation between VEGF and LHR (P < 0.00001, r = 0.78), and it was possible to detect a higher LHR expression in D4 of GT than GC (p < 0.05), moreover this expression was higher in D4 and D8 than D12 of the GT group. bFGF was also expressed in luteal tissue in all days of both groups, however without statistical differences. It was concluded that deslorelin acetate was effective to induce ovulation in mares which did not alter the physiological responses of the corpus luteum in respect of the plasma progesterone concentration, however, affected luteal parenchyma vasculature. Also, LHR and VEGF expression was influenced by induced ovulation, with a lower level at D12, what must be expected in non-pregnant mares. / Muito tem sido feito no intuito de potencializar a aplicação e os resultados das biotécnicas de reprodução assistida na espécie equina, no entanto ainda são escassos os estudos sobre vascularização do corpo lúteo (CL) e sua relação com a progesterona em éguas induzidas com acetato de deslorelina; bem como, sua relação com os fatores angiogênicos e suas consequências quando a ovulação é induzida. Foram utilizadas éguas com ovulação natural (GC) e induzida (GT) com acetato de deslorelina (folículo >35mm). No primeiro experimento, os ovários foram acompanhados por ultrassonografia Doppler (6,5 MHz), nos dias 4, 8 e 12 após a ovulação (D0), quando também foram obtidas amostras de sangue para dosagem de progesterona. Os CLs foram analisados quanto a sua vascularização e as imagens gravadas em vídeo. A análise das imagens considerou o percentual de área do parênquima luteal que estava apresentando sinais coloridos. A concentração de progesterona foi analisada por enzima-imunoensaio. Os resultados foram apresentados em média ± erro padrão da média. As médias de vascularização foram no GC: 76,25 ± 8,98; 70,00 ± 8,16 e 57,50 ± 13,31% e no GT: 85,00 ± 5,40; 90,00 ± 0,00 e 58,75 ± 9,24%, respectivamente para os dias 4, 8 e 12 após a ovulação. As concentrações de progesterona foram no GC: 6,21 ± 0,61; 5,82 ± 1,44 e 4,63 ± 0,79 ng/mL e no GT: 6,10 ± 1,18; 4,67 ± 1,06 e 4,49 ± 1,37 ng/mL respectivamente para os dias 4, 8 e 12 após a ovulação. Os percentuais de vascularização apresentaram valores superiores nos dias 4 e 8 comparados ao D12 do GT (P<0,05), não havendo diferença nos dias do GC, nem entre os grupos a cada momento estudado. As concentrações de progesterona não apresentaram diferenças significativas entre grupos ou momentos estudados. No segundo experimento, foram tomadas amostras do CL através de biópsia guiada por ultrassom (5 MHz), nos mesmos períodos, e os fatores angiogênicos analisados pela expressão do mRNA dos genes VEGF, BFGF e receptor de LH (LHR) utilizando PCR em tempo real. Foi observada correlação positiva entre VEGF e LHR (P < 0,00001, r = 0,78). A expressão do LHR no D4 foi maior no GT do que no GC (p < 0,05). Também observou-se maior expressão nos D-4 e D-8 do que o D-12 do GT. A expressão do VGEF declinou no D12 comparado aos dias D4 e D8 do GT (P = 0,06). O bFGF foi expresso em todos os momentos dos dois grupos, porém sem diferença significativa. Conclui-se que o acetato de deslorelina, foi eficiente para a indução de ovulação em éguas, não alterando as respostas fisiológicas do corpo lúteo no que diz respeito a concentração plasmática de progesterona. No entanto, aumentou a responsividade do CL ao LH através de seus receptores, principalmente no quarto dia após a ovulação. Também a expressão do gene do VGEF foi influenciada pela indução da ovulação, tendo uma queda mais acentuada aos 12 dias após a ovulação, o que deve ser esperado em éguas não prenhes.