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Uridinediphosphate-glucuronosyltransferase (UDP-GT) Ontogeny and PCB Effects in Galliform BirdsMcCleary, Ryan J. R. 06 December 2001 (has links)
Hepatic UDP-GTs are partly responsible for metabolism of the thyroid hormone, thyroxine (T4), in mammals, but little is known of UDP-GT activity in birds. To determine the ontogenic pattern of UDP-GT activity in precocial birds, we measured activity in Japanese quail (Coturnix japonica) liver at days 12 and 14 of the 16.5-day incubation, 3 perihatch stages and <1, 1, 4, 6, 7, 20 and 42 days posthatch. We used an enzymatic reaction with para-nitrophenol (pNP) as substrate that was validated for quail tissue. The pattern of UDP-GT development included low embryonic activity, increased activity beginning in the perihatch period, a peak in activity at day 4 posthatch and a return to lower activity levels from day 6 to adults. The profile of UDP-GT activity, in relation to the ontogeny of circulating T4 and triiodothyronine (T3) in quail, is consistent with UDP-GT playing a role in regulating circulating T4 and with the perihatch peak in T3 stimulating the posthatch peak in UDP-GT activity. To examine the effects of polychlorinated biphenyls (PCBs) on UDP-GT in developing precocial birds, we dosed chicken (Gallus domesticus) eggs with concentrations of PCB 126 from 0 to 0.80 ng/g egg (in sunflower oil) prior to incubation. Tissues were sampled at day 20 of the 21-day incubation and assayed for plasma hormones and UDP-GT activity. Eggs also were dosed with 0 or 0.25 ng PCB 126/g egg or with 0 or 0.64 ng/g egg of the coplanar PCB 77, allowed to hatch, and sampled at 42 days posthatch. There was no consistent pattern of altered thyroid hormones or UDP-GT activity in developing chickens exposed to either of these coplanar PCBs although previous studies indicated developmental alterations from exposure to the higher doses. / Master of Science
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Imunnoresponsiveness in Japanese quail and chickensMiller, Libbie L. 07 April 2009 (has links)
The association between selection for body weight and immune responsiveness was studied in Japanese quail and White Plymouth Rock chicken populations. Quail populations consisted of a randombred control (C) line and a line selected for high (HW) 28-day body weight. The chicken populations used were lines selected for high (HW) and low (LW) 56-day body weight, reciprocal Fi crosses (HL and LH), and F2 crosses of the Fi (HLHL and LHLH).
Kinetics of primary and secondary antibody response to SRBC antigen was examined in Line C quail (Experiment 1). At most times post-primary inoculation (PPI), antibody titers were highest for antigen concentration 2.5%. The presence of MER antibodies was very low PPI, but increased following reinjection. Primary antibody response was then compared between C line and HW line of quail (Experiment 2). Antigen concentration 2.5% once again resulted in the highest titers. Line HW quail were less able to maintain high antibody titer levels to SRBC antigen than the randombred control line from which they originated.
Mode of inheritance for immune responsiveness in selected populations of chickens and crosses between them was examined (Experiment 3). Additive genetic variation was important in the inheritance of both primary and secondary responses to this antigen. Reciprocal differences and heterosis of the F₁ crosses were also factors in the inheritance of secondary response. Kinetics of primary and secondary responses were evaluated in the parental weight lines and in lines of White Leghorn chickens divergently selected for antibody response to SRBC antigen. At all times PPI, line HA chickens had the highest antibody titers, while those from line LA consistently had the lowest titers. Lines HW and LW reacted similarly to line LA early in response, but showed higher peak levels later on. In both primary and secondary responses, the weight lines peaked at similar levels. Thereafter, line LW maintained a high antibody titer level to SRBC antigen than line HW. / Master of Science
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Dissecting Phenotypic Variation in Pigmentation using Forward and Reverse GeneticsHellström, Anders R January 2010 (has links)
Coat color and patterning phenotypes have been extensively studied as a model for advancing our understanding of the relationship between genetic and phenotypic variation. In this thesis, genes of relevance for pigment cell biology were investigated. The dissertation is divided in two parts. Forward genetics was used in the first part (Paper I and II) to identify the genes controlling the Silver and Sex-linked barring loci in chicken. In the second part, reverse genetics was employed to create a mouse line in which the PMEL17 protein is inactivated (Paper III). In Paper I, we report five mutations in SLC45A2 causing plumage color variants in both chicken and Japanese quail. Normal function of the SLC45A2 gene has previously been shown to be essential for the synthesis of both red/yellow pigment (pheomelanin) and brown/black pigment (eumelanin) in numerous species, including humans. The major discovery in this paper is the specific inhibition of pheomelanin in Silver chickens, whilst null mutations at this locus cause an almost complete absence of both pheomelanin and eumelanin. In Paper II, we report that Sex-linked barring in chickens is controlled by the CDKN2A/B tumor suppressor locus. The locus encodes two proteins, INK4B and ARF. The genetic analysis indicates that missense mutations in ARF or mutations in the promoter region of the ARF transcript are causing Sex-linked barring. In previous studies, mutations inactivating the CDKN2A/B tumor suppressor locus, have been shown to be responsible for familiar forms of human melanoma. Here we propose that these mutations in chicken CDKN2A/B cause the premature cell death of melanocytes as opposed to the cell proliferation and tumor growth associated with loss-of-function alleles in humans. In Paper III, we created a mouse line in which the PMEL17 protein is inactivated. Missense mutations in the gene encoding PMEL17 have previously been shown to be associated with reduced levels of eumelanin in epidermal tissues in several vertebrate species. The knockout mice are viable, fertile, and display no obvious developmental defects. The eumelanosomes within the melanocytes of these mice are spherical in contrast to the cigar-like shaped eumelanosomes present in wild-type animals. PMEL17 protein inactivation has only a subtle diluting effect on the coat color phenotype in four different genetic backgrounds. This suggests that other previously described alleles in vertebrates with more striking effects on pigmentation are dominant-negative mutations.
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DESEMPENHO E PARÂMETROS REPRODUTIVOS DE DIFERENTES LINHAGENS DE CODORNAS JAPONESAS (Coturnix coturnix japonica) / Performance and reproductive parameters of different strain of japonese quail (coturnix coturnix japonica)GOMES, Natali Almeida 28 March 2008 (has links)
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Previous issue date: 2008-03-28 / It was the objective of this study to evaluate the performance and reproductive
parameters of different genetic groups of japanese quails between six and 26 weeks
of age. There were used 384 japanese quails, assigned according to the experimental
design (ramdomized blocks), considering the initial live weight of the birds as blocks
in the beginning of the experiment. The treatments were three genetic groups with
eight replicates, in a total of 48 experimental units with eight birds in each
experimental unit (two males and six female). The birds were fed rations according to
recommendations of NRC (1994), having soybean meal and ground corn as the main
components. The variables of performance were studied every 14 days and three
incubation trials were done with 15, 24 and 33 weeks of age. For the statistical
analysis the proc GLM of SAS software was used and the means were compared by
the t Student test. In each incubation study the completely ramdomized blocks
experimental design was used, considering the incubating machines as blocks, with
70 replicates per treatment and one egg as the experimental unit. The parameters
evaluated were the egg weight, hatchability, chick weight at hatching, weight loss and
weight after six hours of fasting. The results were submitted to variance analysis and
the means compared by the t Student test (5% of probability). The genetic groups
presented differences among each other for the live body weight in the beginning of
production, age at 50% of of productiontwere different to body weight at the beggining
and age at 50% of production, where the commercial parent line obtained the best
performance. The parameters peak persistence, eggs production, feed intake,
viability and beak behavior did not present differences among the genetic groups. The
live body weight influenced the egg weight. In the incubation trials the commercial
parent line presented more weight of the incubating egg and more weight of the
chicks at hatching. It was concluded in the present study that there are statistical
differences on the performance and incubating parameters among genetic groups of
japanese quails. / O objetivo deste estudo foi avaliar o desempenho e parâmetros reprodutivos de
diferentes grupos genéticos de codornas japonesas entre seis e 26 semanas de
idade. Foram utilizadas 384 codornas japonesas, distribuídas de acordo com
delineamento experimental em blocos casualizados, considerando como blocos os
pesos das aves no início do experimento. Os tratamentos estudados foram três
grupos genéticos, com oito repetições, totalizando 48 parcelas com oito aves cada
(dois machos e seis fêmeas). As aves receberam rações formuladas a partir de
recomendações contidas no NRC (1994) tendo como base farelo de soja e milho
moído. As variáveis de desempenho foram estudadas a cada período de 14 dias e
foram realizados três ensaios de incubação, com 15, 24 e 33 semanas de idade.
Para a análise estatística utilizou-se o procedimento GLM do programa SAS e as
médias comparadas pelo teste t de Student. Em cada ensaio de incubação, foi
adotado o delineamento experimental em blocos casualizados, sendo considerados
blocos as incubadoras, com 70 repetições por tratamento, sendo considerado um
ovo a parcela. As variáveis estudadas foram peso do ovo, eclodibilidade, peso ao
nascer, perda de peso e peso no alojamento. Os resultados foram submetidos à
análise de variância e as médias comparadas pelo teste t de Student (5% de
probabilidade). As linhagens apresentaram diferenças entre si para peso ao início de
produção e idade aos 50% de produção, sendo que a linhagem comercial apresentou
os melhores resultados. Para as variáveis persistência de pico, produção de ovos,
consumo de ração, viabilidade e comportamento de bicagem não foram observadas
diferenças entre as linhagens. O peso das aves influenciou o peso do ovo. Nos
ensaios de incubação, a linhagem comercial apresentou maior peso de ovo incubável
e maior peso ao nascer das codorninhas. Conclui-se no presente estudo que há
diferenças significativas para desempenho e parâmetros de incubação entre as
linhagens de codornas japonesas estudadas.
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