Global ETD Search

541	Development of Novel High-Resolution Melting (HRM) Assays for Gender Identification of Caribbean Flamingo (Phoenicopterus ruber ruber) and other Birds Chapman, Alexandra 14 March 2013 (has links) Unambiguous gender identification (ID) is needed to assess parameters in studies of population dynamics, behavior, and evolutionary biology of Caribbean Flamingo (Phoenicopterus ruber ruber) and other birds. Due to its importance for management and conservation, molecular (DNA-based) avian gender ID assays targeting intron-size differences of the Chromosome Helicase ATPase DNA Binding (CHD) gene of males (CHD-Z) and females (CHD-W) have been developed. Male (ZZ) and female (WZ) genotypes are usually scored as size polymorphisms through agarose or acrylamide gels. For certain species, W-specific restriction sites or multiplex polymerase chain-reaction (PCR) involving CHD-W specific primers are needed. These approaches involve a minimum of three steps following DNA isolation: PCR, gel electrophoresis, and photo-documentation, which limit high throughput scoring and automation potential. In here, a short amplicon (SA) High-resolution Melting Analysis (HRMA) assay for avian gender ID is developed. SA-HRMA of an 81-Base Pair (bp) segment differentiates heteroduplex female (WZ) from homoduplex male (ZZ) genotypes by targeting Single-nucleotide Polymorphisms (SNPs) instead of intron-size differences between CHD-Z and CHD-W genes. To demonstrate the utility of the approach, the gender of Caribbean Flamingo (P. ruber ruber) (17 captive from the Dallas Zoo and 359 wild from Ria Lagartos, Yucatan, Mexico) was determined. The assay was also tested on specimens of Lesser Flamingo (P. minor), Chilean Flamingo (P. chilensis), Saddle-billed Stork (Ephippiorhynchus senegalensis), Scarlet Ibis (Eudocimus ruber), White-bellied Stork (Ciconia abdimii), Roseate Spoonbill (Platalea ajaja), Marabou Stork (Leptoptilos crumeniferus), Greater Roadrunner (Geococcyx californianus), and Attwater's Prairie Chicken (Tympanuchus cupido attwateri). Although the orthologous 81 bp segments of Z and W are highly conserved, sequence alignments with 50 avian species across 15 families revealed mismatches affecting one or more nucleotides within the SA-HRMA forward or reverse primers. Most mismatches were located along the CHD-Z gene that may generate heteroduplex curves and thus gender ID errors. For such cases, taxon and species-specific primer sets were designed. The SA-HRMA gender ID assay can be used in studies of avian ecology and behavior, to assess sex-associated demographics and migratory patterns, and as a proxy to determine the health of the flock and the degree by which conservation and captive breeding programs are functioning. endangered birds gender of sexually monomorphic birds sexually monomorphic birds fledglings nestlings DNA test to sex birds Attwater's Prairie Chicken Accipiter species Ría Lagartos Biosphere Reserve Lesser Flamingo Flamingo Chilean Flamingo Phoenicopterus ruber ruber Phoenicopterus Caribbean Flamingo High-resolution Melting Analysis CHD1-W CHD1-Z polymorphism W chromosome Z chromosome CHD1 gene CHD gene gender of birds sex determination of birds gender determination of birds multiple avian species non-invasive conservation of birds sex-ratio of birds sex determination molecular avian gender molecular gender determination Universal sexing of non-ratite birds
542	Grassroots Canadian Muslim Identity in the Prairie City of Winnipeg: A Case Study of 2nd and 1.5 Generation Canadian Muslims Hameed, Qamer January 2015 (has links) What are grassroots “Canadian Muslims” and why not use the descriptor “Muslims in Canada”? This thesis examines the novel concept of locale specific grassroots Canadian Muslim identity of second and 1.5 generation Muslims in the prairie city of Winnipeg, Manitoba. The project focuses on a generation of Muslims that are settled, embedded, and active in a medium sized Canadian metropolis. Locale plays a powerful part in the way people navigate identities, form attachments, find belonging, and negotiate communities and society. In order to explore this unique identity a case study was conducted in Winnipeg. Interviews with 1.5 and second generation Muslims explored the experience of grassroots Canadian Muslim identity. The project does not focus on religious doxy or praxis but rather tries to understand a lived Canadian Muslim identity by exploring discourse and space as well as strategies, social perceptions and expectations. Participant observation, community resources and literature also aid in the understanding of the grassroots Canadian Muslim experience. This study found that the attachments, networks, and experiences in the locale give room for an embedded Canadian Muslim experience and more negotiable identities than most studies on Muslims in Canada describe. These individuals are not foreigners living in Canada. Their worldviews develop out of this particular and embedded grassroots experience. They navigate a new kind of hybrid Canadian Muslim identity that is unique and flexible. This is the Canadian Muslim experience of 2nd and 1.5 generation Winnipeg Muslims. Muslim Canadian Muslims Canadian Muslim Muslims in Canada Muslim Canadian second generation 1.5 generation minorities minority Canadian-Muslim immigrant immigrants diversity pluralism multiculturalism hybrid identity identities social society mosque community locale grassroots prairie Winnipeg Manitoba religion culture religious discourse generation migrant settlement social movement organization citizenship ethnicity nationality nation woman women Muslim women hijab veil 2nd Generation 1st generation first generation third generation Islamophobia dicrimination immigration immigrant transnational liberal conservative youth spatial analysis lived religion
543	Comparison of Bare Root vs. Potted Plants, Species Selection, and Caging Types for Restoration of a Prairie Wetland, and Quantitative Analysis and Descriptive Survey of Plant Communities and Associations at Lewisville Lake Environmental Learning Area (LLELA), Lewisville, TX Buckallew, Robin R. 05 1900 (has links) Lewisville Lake Environmental Learning Area (LLELA) is an 809-hectare property in Denton County, TX. A study of the vegetation community identified 466 species in 104 families, with 25% of the species from only two families, Asteraceae and Poaceae. The property demonstrates the characteristics of an early successional community, dominated by weedy species. Prairie communities are dominated by Johnson grass and ragweed, with climax tall grass prairie communities only in areas that have been planted with native grass seed. Forest communities are similarly in an early successional stage, dominated by the hackberry-elm-ash alliance, with small remnants of native Cross Timbers found in isolated patches. Species richness and diversity were highest in the forests and lowest in the wetlands; evenness, though not different across ecosystems, demonstrated a strong seasonal component. The species list was compared with previously reported lists for Denton County, and 256 species identified had not been previously reported for the county. A wetland restoration study was conducted to determine if there was a difference in survival and growth between potted transplants with intact root systems and bare-root transplants. Two different mesh sizes were used for protection, and the success of the different caging was evaluated. Of eight species, only four survived through the second growing season. There was no significant difference in the success of the propagule types for Sagittaria latifolia. The treatments planted with intact root systems showed significantly higher growth and reproduction than the bare-root treatments for Eleocharis quadrangulata, Heteranthera dubia, and Vallisneria americana. There was no survival recorded in the coarse mesh cages, likely due to the presence of crayfish that are able to get through the coarser mesh and feed on the transplants. Wetland restoration planting techniques LLELA Denton County vegetation biodiversity Lake Lewisville
544	Playing, learning, and using music in early Middle Indiana Peterson, Erik C. January 2013 (has links) Indiana University-Purdue University Indianapolis (IUPUI) / This thesis is a study of how people in the nine counties of central Indiana learned, appreciated, and performed music from 1800 to 1840. A concluding proposal for a public history application of this research is included. music in Indiana 1800-1840 Conner Prairie (Museum) Indiana Historical Society
545	The Saskatchewan adult attendance centre project (1979-84) : a case history Collier, Dilys Mary 25 October 2010 The purpose of this case history was to view the development of the Saskatchewan Adult Attendance Centre Project through the perspective of currently accepted, but selected, adult education philosophy, principles, and techniques. The Project was a mandatory adult education component of Probation Services, a program for adult offenders operated by Saskatchewan Corrections. The story of the evolution from 1979 to 1984 of the two Adult Attendance Centres of the Project, based in the cities of Regina and Saskatoon, was presented in the context of an historical overview of the education of adults in the Corrections systems of Britain, the United States, and Canada. The Attendance Centres were not set up as adult education institutions. They were intended to be cost effective alternatives to incarceration. The study maintained that sentencing that included attendance at the Centres was more cost effective for the provincial government than incarceration or traditional probation. It argued that the kind of education presented to adult probationers in the Centre programs often strayed from currently accepted adult education philosophy, principles, and techniques. None the less, significant potential existed in the Centres for the creation of more meaningful adult education opportunities for persons on probation. justice John Howard Society John Howard incarceration Federal correctional policy in Canada English prisons English prison education Federal correctional policy Elizabeth Fry Elizabeth Fry Society Dilys Collier Canadian justice system Corrections Saskatoon corrections Corrections Canada correctional system criminal justice system Canada's penitentiary system case history British correctional system Bridewell Borstal Bridewell schools British adult education British Prison Education Australian prisons Australian attendance centre Australian adult education adult continuing education adult attendance centre attendance centre adult education principles justice system adult education Saskatchewan correctional system Saskatchewan Justice Saskatchewan Department of Justice Otto Dreidger Prairie Justice Research penitentiaries in the United States parole prison education probation probationers probation services Robert A. Carlson Michael Collins
546	The Saskatchewan adult attendance centre project (1979-84) : a case history Collier, Dilys Mary 25 October 2010 (has links) The purpose of this case history was to view the development of the Saskatchewan Adult Attendance Centre Project through the perspective of currently accepted, but selected, adult education philosophy, principles, and techniques. The Project was a mandatory adult education component of Probation Services, a program for adult offenders operated by Saskatchewan Corrections. The story of the evolution from 1979 to 1984 of the two Adult Attendance Centres of the Project, based in the cities of Regina and Saskatoon, was presented in the context of an historical overview of the education of adults in the Corrections systems of Britain, the United States, and Canada. The Attendance Centres were not set up as adult education institutions. They were intended to be cost effective alternatives to incarceration. The study maintained that sentencing that included attendance at the Centres was more cost effective for the provincial government than incarceration or traditional probation. It argued that the kind of education presented to adult probationers in the Centre programs often strayed from currently accepted adult education philosophy, principles, and techniques. None the less, significant potential existed in the Centres for the creation of more meaningful adult education opportunities for persons on probation. justice John Howard Society John Howard incarceration Federal correctional policy in Canada English prisons English prison education Federal correctional policy Elizabeth Fry Elizabeth Fry Society Dilys Collier Canadian justice system Corrections Saskatoon corrections Corrections Canada correctional system criminal justice system Canada's penitentiary system case history British correctional system Bridewell Borstal Bridewell schools British adult education British Prison Education Australian prisons Australian attendance centre Australian adult education adult continuing education adult attendance centre attendance centre adult education principles justice system adult education Saskatchewan correctional system Saskatchewan Justice Saskatchewan Department of Justice Otto Dreidger Prairie Justice Research penitentiaries in the United States parole prison education probation probationers probation services Robert A. Carlson Michael Collins

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