To study the distribution of Clostridium perfringens and toxin genes, 48 swine farms were visited and 354 fecal samples were collected. The isolates recovered from lactating sows, gestating sows, grower-finishers, and manure pits were less likely to possess consensus gene cpb2 compared to those from suckling pigs (P<0.05). The relative importance of different pathogens associated with neonatal piglet diarrhea was identified. A total of 237 neonatal diarrhea cases were submitted to the Animal Health Laboratory, University of Guelph between 2001 and 2010. The combined frequencies for cases involving enterotoxigenic Escherichia coli, Clostridium perfringens type A, rotavirus, and Clostridium difficile accounted for 56% of the total cases. A survey was administered to 22 practitioners and 17 pathologists for the diagnosis of C. perfringens type A. The majority (95%) of practitioners were moderately to very confident of their diagnosis, but almost half (41%) of the pathologists were not confident of their diagnosis. / Ontario Ministry of Agriculture Food and Rural Affairs

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Endotoxins or lipopolysaccharides (LPS), highly toxic component of the outer membrane of gram-negative bacteria, are normally present in the mammalian gut lumen.In this thesis, I investigated, in laboratory animals, whether these gut-derived endotoxins play a role in pathophysiology resulting from intestinal dysfunctions caused by intestinal ischaemia and heat-stress.In primates, reperfusion of the splanchnic region after a temporary ischaemia was followed by a rapid increase in LPS concentration, first in the hepatic portal plasma and, ten minutes later, in the systemic arterial plasma. Rises in plasma LPS concentrations during or following the temporary intestinal ischaemia was prevented by prophylactic administrations of corticosteroids, anti-LPS IgG antibodies and oral, non-absorpable, antibiotics agents which appear to stabilize cellular membranes, aid the reticuloendothelial system in removal of LPS from the circulation and destroy the intestinal aerobic gramnegative bacteria respectively. In addition, administration of therapeutic anti-LPS antibodies also rapidly reduced the plasma LPS concentrations to baseline during an endotoxaemia. In a control heat-stress model, elevations in plasma LPS concentration commenced at rectal temperatures greater than 41,SoC. Like the intestinal ischaemia model, this occurred first in the hepatic portal plasma, and 10-15 minutes later, in the systemic arterial plasma. Peak plasma LPS levels of about 0,3 ng/ml, measured in heat-stressed primates, have proved in previous studies, to be toxic. A rapid decline in mean arterial pressure was followed by increases in plasma LPS concentrations and heart rates. Reductions in splanchnic blood flow and consequent local ischaemia coupled with thermal injury to the intestinal wall and the liver, may have permitted rises in plasma LPS concentration. Furthermore, as in the ischaemia model, prophylactic administrations of corticosteroids, anti-LPS IgG antibodies, and oral, nonabsorbable antibiotics prevented a rise in plasma LPS concentration. Of importance, prophylaxis with intravenous corticosteroids and 'anti-LPS IgG antibodies increased the survival rates significantly in heat stroke in primates. In addition, monkeys having high titres of "natural" antiLPS IgG antibodies had lower plasma LPS concentrations and survived the induced-heat stroke. It is suggested that other pathophysiologic conditions which compromise the integrity of the gut wall would also lead to the development of an endotoxaemia, and that gutderived endotoxins contribute to the athogenesis of heat stroke and treatments with corticosteroids and anti-LPS IgG antibodies may prove beneficial in other endotoxinrelated disorders. / Thesis (Ph.D.)-University of Natal, Durban, 1988.

The susceptibility of 80 StaphyIococcus aureus isolated to oxacillin was investigated using microtitre, agar dilution and Stokes' disc diffusion methods. There was a bimodal distribution of the isolates according to the oxacillin minimum inhibitory concentration (MIC) values. For the sensitive isolates, the agar dilution method generally gave lower MIC values than the microtitre method, while for the resistant isolates the agar dilution method gave comparable to slightly lower MIC values than the microtitre method. The Stokes disc diffusion method yielding the best results when performed on Mueller-Hinton agar incubated at 30°C for 18 hours; however local strains grew poorly when incubated at 30 C for 18 hours. The next best medium which provided clear disc diffusion results plus good growth was Mueller-Hinton agar incubated at 35°C for 18 hours, on which 10 % of the sensitive isolates appeared intermediate in susceptibility, and none resistant, while all the resistant isolates (microtitre MIC >8mg/1) appeared resistant. Oxacillin resistance among strains of Staphylococcus aureus tested by Stokes' disc diffusion method correlated best with gentamicin resistance, and less often with tetracycline resistance. Therefore gentamicin- or tetracycline-resistance may indicate oxacillin resistance in Staphylococcus aureus. / Thesis (M.Med.)-University of Natal, Durban, 1988.

During the past decade the environment of learning has been changing notably due to the expansion of information and communication technologies. New forms of working appear and the improvement methods of education and skills change. Teaching with modern technologies has become an obligatory aspect of remit. Consequently, the lecturers seek for new methods of education to meet the increasing demand of students and improve the quality of education. Regarding the demand for education shift on the basis of working market, the goal of the paper is to analyze and ground the e-laboratory application for “Information processing systems” module in the laboratories. The paper analyses an idea in essence, which is crucial and modern in Lithuania as well as abroad – a new environment purposing the formation of environment in the e-laboratory. On the basis of the latter module of e-tools and e-learning systems of teaching experience in digital signal teaching course, the recommendations for its formation methodology to reach the comprehensive e-laboratory has been proposed. Hopefully, the application of e-laboratories will enable to decline the licensed software in future, meet the increasing demand of the students, grant the quality of education and organize the module for distance learning.

A movement from the traditional to the modern in geoscience education occurs through piecemeal application of educational theory to geology teaching. This dissertation developed and examined four traditional and innovative geoscience skills-based learning activities through qualitative, quantitative and mixed-methods methods: A. Mineralogy laboratories were designed to improve learning gains (i.e., knowledge) and students’ perceptions of mineralogy topics, primarily using group work. Groups of sizes 3 and 4 were most effective (compared with pairs, and groups of 5 and 6) in improving student collaboration. B. An inquiry-style videogame was designed and tested in order to compare learning gains to that of a geological field trip. Though learning gains were slightly higher in the fieldtrip, some aspects of the videogame were more successful at increasing the depth and awareness of observation skills needed. C. Field notebooks were analysed for uniqueness and completeness to quantify differences among participants’ note-taking. We found that previous geologic experience, gender, and lecturer teaching styles all contributed to the students note taking abilities and perceptions of note-taking. D. The design research of the Volcanic Hazards Simulation resulted in identification of critical pedagogical variables that encourage students’ transferable skills: a) the pace of the simulation, b) the preparedness of the students, c) the role and team authenticity and d) communication best practices. Meaningful changes to the curriculum of labs, field and experiential teaching methods resulted in the improvement of content knowledge, perceptions and skills of geoscience students. Collectively, these results suggest practical and theory-based solutions grounded in Constructivist paradigms to provide improved geoscience teaching at Universities.

The research presented in this thesis studies the effects of foamed bitumen on the deformational behaviour and performance of pavement materials. The research was conducted in the laboratory and the field, using specific New Zealand materials. The aggregate used is a blend of a coarse aggregate imported from the Auckland region with a crushed dust from the Canterbury region. The bitumen selected for the study is an 80/100 bitumen grade, and the active filler was a Portland Cement, both commonly used for foamed bitumen stabilization in New Zealand. In the laboratory, samples of mixes with different foamed bitumen content were tested under various loading and stress conditions to investigate the effects of foamed bitumen on the deformational behaviour of the mix. The tests performed were: Indirect Tensile Strength (ITS), Indirect Tensile Resilient Modulus (ITM), Repeat Load Triaxial compression (RLT) and Monotonic Load Triaxial compression (MLT). Preliminary ITS and RLT tests conducted on mixes with 1% and 0% cement, at different foamed bitumen contents, indicated that mixes without cement performed poorly compared to the mixes with 1% cement. Therefore, the rest of the laboratory study was on mixes with 1% cement. ITS tests were conducted on 150 mm specimens prepared with 0% 1%, 2%, 3% and 4% bitumen content, with a common 1% cement. Results indicated that foamed bitumen increases the ITS values of the mix, up to an estimated optimum of 2.8% bitumen content. Similar trends were obtained with ITM tests, in which a diametrical load pulse was applied on 150 mm specimens, showing an estimated resilient modulus peak near to 2.8% bitumen content. RLT specimens were prepared at 0%, 2% and 4% bitumen content, at two compaction efforts, creating specimens at low and high bulk density. Permanent deformation RLT tests involved the application of seven stages of 50,000 load cycles each (4 Hz), with increasing deviator stress (from 75 kPa in the first stage, up to 525 kPa in the seventh stage) and at constant confining pressure of 50 kPa. Results of RLT permanent deformation tests indicated that the increase in the foamed bitumen content resulted in an increase in the permanent deformation of the material. MLT tests were conducted on specimens at 0%, 2% and 4% bitumen contents, at two compaction efforts, creating specimens of low and high bulk density, at confining pressures ranging from 50 kPa to 300 kPa, with a deformation rate of 2.1% per minute. Results indicated that the effect of foamed bitumen was a reduction of the peak vertical stress, or a reduction in the peak strength. The peak stresses obtained in MLT tests were plotted in stress diagrams, and the failure was approximated as linear function of the confining stress. The fundamental shear parameters (angle of internal friction and apparent cohesion) were estimated, and results indicated that foamed bitumen has no apparent effect in cohesion but does reduce the angle of internal friction. The reduction of the angle of internal friction explains the general trends observed in the laboratory, that on one hand the compressive strength decreases with increasing bitumen content, but on the other hand, the tensile strength increases up to an optimum. A full-scale experiment was carried out using an accelerated testing of foamed bitumen pavements at the Canterbury Accelerated Pavement Testing Indoor Facility (CAPTIF). In the full-scale experiments, the same materials that were tested in the laboratory (aggregates, bitumen, cement) were used to construct six different pavement sections, each with different contents of bitumen and cement. Three were constructed using foamed bitumen contents of 1.2%, 1.4% and 2.8% respectively, plus a common active filler content of 1.0% cement. Two more pavements were constructed adding cement only (1.0%), and foamed bitumen only (2.2%). In addition, one control section with the untreated unbound material was tested. Strains were collected using a 3D Emu soil strain system installed in each pavement section. The curing time between construction and pavement loading was approximately three months. The pavement response, such as surface deformation (rutting), surface deflections and strains were periodically recorded during the execution of the test. The strains were collected at different depths by using an array of Emu strain gauges. Deflections were recorded using both a Falling Weight Deflectometer (FWD) and CAPTIF Beam deflectometer, which is a modified Benkelmann beam. A total number of approximately 5.6 million equivalent standard axles were applied on the pavement sections. The rutting measured in the sections stabilised with foamed bitumen and cement was the lowest, showing that the addition of foamed bitumen significantly improved the performance of materials with 1% cement. The sections stabilised with cement only, foamed bitumen only, and the control untreated section showed large amounts of rutting and heaving by the end of the test. Deflection measurements showed that the effect of foamed bitumen content is a reduction of pavement deflections, with the lowest deflection measured in the section stabilised with 2.8% bitumen and 1% cement. The elastic pavement strains showed that foamed bitumen reduced the tensile strains in the basecourse but did not have a significant effect on vertical compressive strains. During the construction of pavements, material samples were taken for ITS and RLT testing. Results indicated that the highest ITS was measured in the section with 2.8% foamed bitumen content and 1% cement, and the ITS in the section without cement and foamed bitumen only was about 4-5 times lower than the ITS measured in specimens with cement. RLT specimens without cement performed poorly in comparison with the specimens with 1% cement. The specimens with 1% cement showed higher permanent deformation with increase in the foamed bitumen content, supporting the results from the previous laboratory study. To interpret and relate the results observed in the laboratory and the field, stress path analysis was used, in which the stress ratio of the foamed bitumen layers was calculated at different depths. The analysis showed that foamed bitumen content decreases the maximum stress ratio, hence reducing the proximity to failure and relative damage of the layer. Three-dimensional and two-dimensional finite element modelling of the CAPTIF pavements, were used to further investigate the stress and strain fields induced by the loading and to explain the pavement performance observed in the full-scale experiment.

Cardiovascular disease is now the leading cause of death worldwide. Serum amyloid A (SAA), a positive acute phase reactant, along with C-reactive protein is used clinically as a marker of cardiovascular disease risk. However, recent data has shed light on a possible causal role of SAA in the development of atherosclerosis, the most pervasive form of cardiovascular disease. Several inflammatory diseases such as diabetes and obesity are known to confer increased risk of developing cardiovascular disease. Individuals with these diseases all have modest but persistent elevation of SAA. To determine if SAA caused the development of atherosclerosis, apoe-/-chow fed mice were injected with either an adenoviral vector expressing human SAA1 (ad-hSAA1), a null adenoviral vector (ad-Null) or saline. Human SAA levels rapidly increased, albeit briefly then returned to baseline within 14 days in mice that received ad-hSAA1. After 16 weeks, mice that received ad-hSAA1 had significantly increased atherosclerosis compared to controls on the aortic intimal surface (p<0.0001), aortic sinus (p<0.05) and the brachiocephalic artery (p<0.05). According to the “response to retention” hypothesis; lipoprotein retention by vascular wall proteoglycans is a key initiating event in the development of atherosclerosis. We previously reported that SAA-stimulated vascular smooth muscle cells expressed biglycan with increased glycosaminoglycan chain length and increased binding affinity for low density lipoprotein. To further test the role of biglycan on the development of atherosclerosis we generated biglycan transgenic mice. These mice were crossed to the ldlr-/- mouse on a C57BL/6 background and fed a pro-atherogenic western diet for 12 weeks. There was a significant increase in atherosclerotic lesion area on the aortic intimal surface (p<0.05) and the aortic sinus (p<0.006), as well as a significant correlation between vascular biglycan content and aortic sinus atherosclerotic lesion area (p<0.0001). These data demonstrate that transiently increased SAA resulted in increased atherosclerosis compared to control mice, possibly via increased vascular biglycan content. In support of this we found that biglycan transgenic mice had significantly increased atherosclerosis compared to wildtype controls, likely through increased lipid retention in the vascular wall.

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