Esta tese procura articular a pesquisa etnográfica em laboratórios de biologia, genética e bioquímica na qual se destacam os recintos como ambientes de domesticação científica de agentes orgânicos ao pensamento da evolução, abordado pela leitura da obra de Charles Darwin, uma vez que a concepção sobre a variação e a conservação das formas orgânicas, nas expressões deste autor, revela-se fundamento das práticas daqueles laboratórios. O estudo aqui proposto busca contribuir para o debate em torno de uma antropologia da ciência e da modernidade, inspirando-se nos escritos Bruno Latour. / This dissertation proposes an articulation between ethnographical research in laboratories (biology, genetics and biochemistry) in which precincts as environments of scientifical domestication of organic agents stands out and evolutionary thought, considered through Charles Darwins works, insofar as the concept of «variation» and «conservation» of «organic forms», in his saying, stands as the fundament of those laboratories practices. This study aims to contribute to an anthropology of science and modernity, inspired by Bruno Latours writings.

A presente pesquisa teve por finalidade analisar se os conhecimentos que os alunos adquirem sobre EducaÃÃo Ambiental (EA), ao final do Ensino MÃdio, atendem ao que preceituam os ParÃmetros Curriculares Nacionais (PCNs) e as Diretrizes Curriculares Nacionais do Ensino MÃdio (DCNEM). A questÃo que orientou essa pesquisa pode assim ser expressa: atà que ponto os alunos que estÃo concluindo o Ensino MÃdio tÃm os conhecimentos bÃsicos sobre EA como proposto pelos PCNs e DCNEM? Desta questÃo central, outras, igualmente relevantes, apresentam-se, como: Qual o conceito de interdisciplinaridade em EA nos PCNs? Qual o conceito de interdisciplinaridade em EA nos DCNEM? O que hà em comum entre os conceitos de EA nos PCNs e DCNEM? O que hà de divergente entre os conceitos de EA nos PCNs e DCNEM? Quais os avanÃos e retrocessos entre as concepÃÃes de EA nos PCNs e DCNEM? Como os conceitos de EA nos PCNs e DCNEM se refletem no fazer educativo da escola? Qual o lugar curricular da EA no Ensino MÃdio? A metodologia da pesquisa foi marcada por uma abordagem indutiva e dedutiva com anÃlise qualitativa. Foi ainda realizado um estudo de campo, como forma de constatar a aprendizagem dos alunos no Ensino MÃdio. A tÃcnica de pesquisa utilizada foi um questionÃrio fechado acerca do carÃter socioeconÃmico dos sujeitos da pesquisa, da prÃtica pedagÃgica dos professores de quÃmica e de avaliaÃÃo de aprendizagem em quÃmica a partir da abordagem pedagÃgica da contextualizaÃÃo e da interdisciplinaridade e sua relaÃÃo com a EA. O pÃblico alvo da pesquisa foram quarenta alunos do 3 ano de uma escola pÃblica da CREDE 1 â MaracanaÃ. A pesquisa chegou à conclusÃo de que os alunos que estÃo concluindo o Ensino MÃdio tÃm os conhecimentos necessÃrios relativos à questÃo ambiental conforme preceituam os PCNs e DCNEM, nutrindo certa consciÃncia ecolÃgica acerca da aplicaÃÃo de seus saberes em aÃÃes ecologicamente corretas. Concluiu ainda que os saberes sobre os conteÃdos de QuÃmica, quando contextualizados e tratados de modo interdisciplinar geram melhoria da aprendizagem. Jà as questÃes especÃficas de quÃmica que poderiam ser potencializada a sua compreensÃo com o uso didÃtico do laboratÃrio apresentaram baixo percentual de acerto, exatamente pelo pouco uso do mesmo e da ausÃncia de uma abordagem investigativa que levasse o aluno à reflexÃo sobre o saber quÃmico. Assim, este trabalho produziu um manual de prÃticas quÃmicas com enfoque ambiental e o objetivo de auxiliar o professor a contextualizar os conhecimentos mais prÃprios da disciplina. / This research aimed to examine whether the knowledge that students acquire on Environmental Education (EA), at the end of high school, attend to what is set down by the PCNs and DCNEM. The question that guided this research can thus be expressed: to what extent do students who are completing high school have the basic knowledge about EA as proposed by the PCNs and DCNEM? From this central issue, other questions, equally relevant, are presented, such as: What is the concept of interdisciplinarity in EA in PCNs? What is the concept of interdisciplinarity in EA in DCNEM? What is in common between the concepts of EA in PCNs and DCNEM? What is divergent in concepts of EA in PCNs and DCNEM? What are the advances and setbacks between the EA conceptions in the PCNs and DCNEM? How are the concepts of EA reflected in PCNs and DCNEM in the educational practice? What is the place of the EA in the high school curriculum? The method of the research was led by and inductive and deductive approach with qualitative analysis. A field study was also carried as a way to observe student learning in high school. The technique used was a closed questionnaire about the participantsâ socioeconomic character, the chemistry teachersâ pedagogical practice and the evaluation of the chemistry learning after the contextualization and interdisciplinarity seen in classroom and their relation with EA. The target audience of the survey was forty students of the 3rd year of a public school CREDE 1 - MaracanaÃ. The research concluded that students who are completing high school have the required knowledge of the environmental issue as set down by PCNs and DCNEM, nourishing certain ecological awareness of the application of their knowledge in environmentally friendly actions. It further concluded that the knowledge about the contents of chemistry, when contextualized and treated in an interdisciplinary manner, generated learning. As for the specific issues of chemistry that could have leveraged their understanding with the usage of didactic chemistry lab, presented a low hit percentage, just because of the little use of the laboratory and the absence of an investigative approach that could lead the student to reflect on chemistry knowledge. This way, this research produced a manual of chemistry practices with environmental focus and the objective of helping the teacher to contextualize the particular knowledge of this discipline.

Esta tese procura articular a pesquisa etnográfica em laboratórios de biologia, genética e bioquímica na qual se destacam os recintos como ambientes de domesticação científica de agentes orgânicos ao pensamento da evolução, abordado pela leitura da obra de Charles Darwin, uma vez que a concepção sobre a variação e a conservação das formas orgânicas, nas expressões deste autor, revela-se fundamento das práticas daqueles laboratórios. O estudo aqui proposto busca contribuir para o debate em torno de uma antropologia da ciência e da modernidade, inspirando-se nos escritos Bruno Latour. / This dissertation proposes an articulation between ethnographical research in laboratories (biology, genetics and biochemistry) in which precincts as environments of scientifical domestication of organic agents stands out and evolutionary thought, considered through Charles Darwins works, insofar as the concept of «variation» and «conservation» of «organic forms», in his saying, stands as the fundament of those laboratories practices. This study aims to contribute to an anthropology of science and modernity, inspired by Bruno Latours writings.

<p>  </p> <p>School-based agricultural education programs use laboratories to develop cognitive, psychomotor, and procedural skills (Phipps et al., 2008). It is important to help preservice teachers develop the ability to design instruction to cultivate skills that are taught in laboratory settings. Shulman (1986) authored a term called Pedagogical Content Knowledge (PCK), which is a teacher’s knowledge of teaching. Animal science dissection was the topic chosen for this study, as PCK is topic-specific (Chan & Hume, 2018). There are many interpretations of PCK. The Refined Consensus Model (RCM) of PCK in Science Education (Carlson et al., 2019) was the conceptual model used in this study, as it is the most recent PCK model, and was developed by experts in science education from multiple countries. This model asserts that PCK is comprised of three realms: Collective PCK (cPCK), Personal PCK (pPCK), and Enacted PCK (ePCK). The first purpose of this study was to describe preservice agriculture teachers' Professional Knowledge Bases (which informs pPCK), before and after instruction, on the topic of animal science dissection in a Laboratory Practices in Agricultural Education (LPAE) course. The second purpose was to describe preservice agriculture teachers' cPCK, after instruction, on animal science dissection in an LPAE course. Content Representations (CoRes), a common tool used for PCK research, were used identify evidence of the Professional Knowledge Bases (PKBs) in preservice agriculture teachers’ instructional planning. Results from this study showed elevated descriptions of Professional Knowledge Bases, and participants collectively gained new ideas and collaboration skills. Overall LPAE dissection experience appeared to push the depth of student thinking and ability to make connections with future learning. Future research recommendations include using the RCM of PCK (Carlson et al., 2019) and CoRes in agricultural education; more PCK research, specifically exploring the development of Curricular Knowledge, on preservice teachers in agricultural education; and PCK research on other topics in agricultural education. It is recommended to not only include PCK development in teacher preparation programs but also have more than one exposure to PCK development.</p>

Avhandlingen undersöker ett centralt steg inom vetenskapliga praktiker: hur laboratorieprov översätts till data som anses giltiga och användbara av forskargemenskapen. Baserat på ett multilokalt fältarbete bestående av observationer och intervjuer i laboratorier för genetisk forskning, visas i avhandlingen hur laboratoriepersonalens yrkesskicklighet, samt normer och ideal om vetenskaplig forskning, formade deras praktiker av att producera giltiga data. Eftersom maskiner var väsentliga i forskningen undersöker avhandlingen också de former av agens som människor och maskiner ansågs bidra med till produktionen av giltiga data; giltighet tolkades som reproducerbarhet av forskarna. Med hjälp av representationsbegreppet och Latours begrepp om inskriptioner och immutable mobiles analyserar avhandlingen arbetet med att förvandla laboratorieprov till giltiga data som en tvåstegsprocess. Proven omvandlades först till rådata som sedan tolkades till data. Personalens huvudsakliga ansträngningar fokuserade i det första steget på att uppnå säkra resultat genom att bekämpa osäkerhet i material och metoder. I det andra steget var det viktigaste att eliminera subjektivitet och att göra objektiva tolkningar av rådatan. Laboratoriepersonalens yrkesskicklighet och användning av maskiner var viktiga verktyg för att eliminiera osäkerhet och subjektivitet. Säkra och objektiva resultat, dvs giltiga data, förväntades uppnås med hjälp av maskiner. Med användning av bl a Barad’s begrepp agential realism analyserar avhandlingen de olika förståelser av människor och maskiner som formade forskarnas praktiker och möjliggjorde skapandet av giltiga data. / This dissertation explores a central step in scientific practices: how samples are turned into data that is considered valid and useful by the research community. Based on multi-sited fieldwork, with observations and interviews at laboratories involved in genetic research, the study focuses on how the laboratory staff’s professional skill, norms, and ideals of scientific research formed their practices of making valid data. As machines were essential for this research, the study also investigates the forms of agency that humans and machines were seen as contributing to the making of valid data; validity being interpreted as reproducibility by the scientists involved. Drawing upon notions of representations as well as Latour’s concepts of inscriptions and immutable mobiles, the study analyzes the practices of transforming samples into valid data as a two-step process. The samples were first turned into raw data, which was subsequently interpreted as data. During the first step, the staff’s central concern was to battle uncertainty in materials and procedures and establish certainty of results, whereas in the second step it was of vital importance to eliminate subjectivity and make objective interpretations of the raw data. Central tools for eliminating uncertainty and subjectivity were the laboratory staff’s professional skill and the use of machines. Certainty and objectivity of results, i.e. valid data, were expected to occur with the help of machines. Drawing upon e.g. Barad’s framework of agential realism, the study analyzes the various understandings of notions of humanness and machineness which shaped scientists’ practices and made the creation of valid data possible.