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A new approach for the isolation of human lactate dehydrogenase-X from human testes /Prapaporn Toowicharanont. January 1976 (has links) (PDF)
Thesis (M.Sc. (Biochemistry)) -- Mahidol University, 1976.
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Biochemical and molecular studies of Lactate Dehydrogenase Isozymes in the freshwater eels, anguilla japonica (Temminck & Schlegel) and Anguilla rostrata (Le Sueur) /Tsoi, Chang-ming, Stephen. January 1994 (has links)
Thesis (Ph. D.)--University of Hong Kong, 1994. / Includes bibliographical references (leaves 90-100).
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Changes in myoglobin and lactate dehydrogenase in muscle tissues of a diving bird, the pigeon guillemot (Cepphus columba), during maturationHaggblom, Lisa Marie January 1987 (has links)
ix, 46 leaves : ill. ; 29 cm
Notes Typescript
Thesis (M.S.)--University of Oregon, 1987
Includes vita and abstract
Bibliography: leaves 37-46
Another copy on microfilm is located in Archives
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Some aspects of the effect of gossypol on antifertility.January 1990 (has links)
by Ng, Shuet Fai. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1990. / Bibliography: leaves 118-137. / ACKNOWLEDGEMENTS --- p.i / ABSTRACT --- p.ii / CONTENTS --- p.iv / Chapter CHAPTER 1: --- GENERAL INTRODUCTION / Chapter I. --- The History of Gossypol --- p..4 / Chapter II. --- The Chemistry of Gossypol --- p..7 / Chapter A. --- Enantiomers of Gossypol --- p.10 / Chapter B. --- Analysis of Gossypol --- p.11 / Chapter III. --- Metabolism and Distribution of Gossypol in Body --- p.12 / Chapter IV. --- The Effect of Gossypol --- p.14 / Chapter A. --- Effect of Gossypol on Different Animal Species --- p.14 / Chapter B. --- Effect of Gossypol on Male Reproductive Organs --- p.16 / Chapter 1. --- Testis and Epididymis --- p.16 / Chapter 2. --- Spermatozoa and Spermatogenic Cells --- p.18 / Chapter C. --- Effect of Gossypol on Reproductive Hormones --- p.20 / Chapter D. --- Effect of Gossypol on Enzymes --- p.22 / Chapter 1. --- Enzymes in Capacitation --- p.23 / Chapter 2. --- The Metabolic Enzymes --- p.24 / Chapter a. --- On Glycolysis --- p.24 / Chapter b. --- On TCA Cycle --- p.26 / Chapter c. --- On Oxidative Phosphorylation --- p.26 / Chapter 3. --- Adenyl Cyclase --- p.26 / Chapter 4. --- Na+/K+-ATPase --- p.28 / Chapter E. --- Other Effects of Gossypol --- p.29 / Chapter V. --- Toxicity of Gossypol --- p.30 / Chapter A. --- General Toxic Effect --- p.30 / Chapter B. --- The Mutagenicity --- p.32 / Chapter VI. --- Clinical Trials of Gossypol --- p.33 / Chapter VII. --- The Purpose of This Study --- p.35 / Chapter A. --- Gossypol and LDH-X --- p.35 / Chapter B. --- Effect of Gossypol on Antioxidant Defense Systems / Chapter C. --- The Influence of Gossypol on Zinc Metabolism --- p.37 / Chapter CHAPTER 2: --- EFFECT OF GOSSYPOL ON LDH-X OF COCK AND DRAKE / Chapter I. --- Introduction --- p.39 / Chapter A. --- General Description of Lactate Dehydrogenase --- p.39 / Chapter B. --- Discovery of LDH-X --- p.40 / Chapter C. --- Properties of LDH-X --- p.41 / Chapter D. --- Role of LDH-X in the Spermatozoa --- p.42 / Chapter E. --- Gossypol and LDH-X --- p.43 / Chapter II. --- Materials and Methods --- p.44 / Chapter A. --- Reagents --- p.45 / Chapter B. --- Methods --- p.45 / Chapter 1. --- The Effect of Gossypol on the Spermatozoa of Cock and Drake --- p.45 / Chapter 2. --- "Measurement of LDH-X Activities in Cock, Drake, Mouse and Rat" --- p.46 / Chapter a. --- Preparation of Samples --- p.46 / Chapter b. --- Enzyme Assay --- p.47 / Chapter III. --- Results / Chapter A. --- Effect of Gossypol on the Spermatozoa of Cock and Drake --- p.48 / Chapter 1. --- Cock --- p.48 / Chapter 2. --- Drake --- p.49 / Chapter B. --- "The LDH-X Activities of Cock, Drake, Rat and Mouse" --- p.51 / Chapter IV. --- Discussion --- p.54 / Chapter CHAPTER 3: --- "THE EFFECT OF GOSSYPOL ON THE METABOLISM OF ZINC IN THE TESTIS, HAIR AND RETINA OF HAMSTER AND MOUSE" / Chapter I. --- Introduction --- p.57 / Chapter A. --- The Physiological Role of Zinc in Animals --- p.57 / Chapter B. --- The Relationship Between Gossypol and Divalentions --- p.58 / Chapter II. --- Materials and Methods --- p.60 / Chapter A. --- Reagents --- p.60 / Chapter B. --- Treatment of Animals for the Zinc Content Study / Chapter 1. --- Detection of Zinc Content in Hamster Hair --- p.61 / Chapter 2. --- "Detection of Zinc Distribution in the Hair, Testis, and Retina in Hamster and Mouse" --- p.62 / Chapter C. --- Determination of Zinc and Iron --- p.63 / Chapter III. --- Results --- p.63 / Chapter A. --- Amount of Zinc in Hamster's Hair --- p.63 / Chapter B. --- "The Distribution of Zinc Content in Hair, Testis and Retina of Hamster and Mouse" --- p.69 / Chapter IV. --- Discussion --- p.71 / Chapter CHAPTER 4: --- EFFECT OF GOSSYPOL ON THE ANTIOXIDANT DEFENSE SYSTEM IN THE TESTIS OF HAMSTER / Chapter I. --- Introduction --- p.73 / Chapter A. --- Oxygen Radicals in Living Cells --- p.75 / Chapter B. --- Oxygen Damage of Spermatozoa --- p.76 / Chapter C. --- Antioxidant Defense System --- p.77 / Chapter 1. --- Enzymatic Antioxidants --- p.78 / Chapter 2. --- Non-Enzymatic Antioxidants --- p.78 / Chapter II. --- Materials and Methods --- p.81 / Chapter A. --- Reagents --- p.81 / Chapter B. --- Experimental Animals --- p.82 / Chapter C. --- The Effect of Scavengers on the Antifertility of Gossypol --- p.83 / Chapter 1. --- Vitamin C --- p.83 / Chapter 2. --- Selenium --- p.84 / Chapter 3. --- Vitamin E --- p.85 / Chapter D. --- Chemical Interaction of Gossypol with Vitamin C and Sodium Selenite --- p.85 / Chapter E. --- Malonaldehyde Assay --- p.85 / Chapter F. --- The Effect of Gossypol on the Antioxidant Defense Enzymes --- p.86 / Chapter 1. --- Drug Treatment --- p.86 / Chapter 2. --- Preparation of Samples for Enzyme Determination --- p.87 / Chapter 3. --- Enzyme Assays --- p.87 / Chapter a. --- Se-GSH-Px --- p.88 / Chapter b. --- Glutathione-S-Transf erase --- p.88 / Chapter c. --- Catalase --- p.89 / Chapter d. --- Superoxide Dismutase --- p.90 / Chapter III. --- Results --- p.90 / Chapter A. --- The Effect of Scavengers on the Antifertility of Gossypol --- p.90 / Chapter B. --- Chemical Interaction of Gossypol with Vitamin C and sodium selenite --- p.91 / Chapter C. --- The MDA Concentration of the Testis of Hamster after Gossypol and Scavengers Injection --- p.101 / Chapter D. --- The Antioxidant Defense Enzymes --- p.101 / Chapter IV. --- Discussion --- p.109 / Chapter CHAPTER 5: --- CONCLUSION AND GENERAL DISCUSSION --- p.113 / REFERENCES --- p.118 / Chapter APPENDIX I --- THE INFLUENCE OF DIETARY LARD ON THE ANT I FERTILITY EFFECT OF GOSSYPOL --- p.138 / Chapter APPENDIX II --- PROTOCOL FOR GOSSYPOL DETERMINATION --- p.158
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Evolution of L-lactate dehydrogenase/£`-crystallin genes among reptiles and aviansLiao, Chen-Hua 11 July 2001 (has links)
L-lactate dehydrogenase (LDH) cDNAs encoding for LDH-A4 (muscle) and LDH-B4 (heart) isozymes from caiman (Caiman crocodilus apaporiensis) belonging to the order Crocodilia were sequenced. The phylogenetic relationships of the newly determined cDNA and their deduced protein sequences, as well as the previously published sequences of vertebrate LDH isozymes were analyzed by various phylogenetic tree construction methods. These results indicated that Chelonia is indeed more closely related to Crocodilia. The divergent times between caiman and alligator, Chelonia and Crocodilia, were estimated to be approximately 36, 177 million years, respectively.
£`-crystallin/Lactate dehydrogenase B cDNA from caiman (Caiman crocodilus apaporiensis), Pekin duck (Anas platyrhynchos), Muscovy duck (Cairina moschata) and Greylag goose (Anser anser) eye lens were sequenced. Accorcding to cDNA sequences, duck lens £`¡Vcrystallin and heart LDH-B are the products of the same gene. In amino acid sequences, two residues Asn-114 and Phe-118 are well conserved in£`-crystallin/ LDH-B among caiman, Muscovy duck and Greylag goose except in Pekin duck which are replaced by glycine residues. The lens protein composition, LDH activity and£`-crystallin/ LDH B4 protein structure of caiman and three avians were analyzed and compared. The results show no significant differences in conformational or enzymatic properties between Pekin duck £`-crystallin and caiman, Muscovy duck and Greylag goose £`-crystallin. The unique replacement of both Asn-114 and Phe-118 by Gly residues in Pekin duck £`-crystallin amino acid sequence might therefore be due to the selective pressure during the recruitment processes of active enzyme into avian lens£`-crystallins.
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Lactate Dehydrogenase and Citrate Synthase activity in cardiac and skeletal muscle of lowland and highland tinamousAira, Naomi January 2013 (has links)
Tinamous (Tinamidae) have the smallest heart in relation to body mass compared to any other flying bird today (Bishop 1997). This means that heart size is likely to restrict aerobic metabolism. Tinamous inhabit areas from sea level to 4800 m a.s.l., which means that the high altitude living species, Nothoprocta ornata (NO), is exposed to hypoxia. In this study the activity of the two metabolic enzymes Lactate Dehydrogenase (LDH) and Citrate Synthase (CS) was measured and the ratio between the enzyme activities calculated to examine if the small heart of the tinamous affects their aerobic/anaerobic metabolism. The activity of the two enzymes was measured in the heart and the gastrocnemius muscle in the three species Nothoprocta ornata (NO), Nothoprocta perdicaria (NP) and Gallus gallus (GG). CS activity was significantly higher in the heart compared to the skeletal muscle and LDH activity was significant higher in the skeletal muscle than in the heart in all three species. The LDH/CS ratio was significantly higher in NO’s skeletal muscle than in chickens but there was no significant difference between species in the heart. The higher ratio in NO´s muscle could be a sign of a higher anaerobic metabolism that is used in the muscles to compensate for the small heart NO have. In conclusion, the Tinamous
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Investigation of the freeze-thawing process for pharmaceutical formulations of a model protein /Hillgren, Anna, January 2002 (has links)
Diss. (sammanfattning) Uppsala : Univ., 2002. / Härtill 5 uppsatser.
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Changes in blood parameters, muscle myoglobin and muscle lactate dehydrogenase of the Common Murre (Uria aalge) during maturationWilliams, Wendy Ann, 1960- January 1992 (has links)
Typescript.
Includes vita and abstract.
Bibliography: Includes bibliographical references (leaves 95-99).
Description: xii, 99 leaves : ill. ; 29 cm. / Blood oxygen carrying capacity, myoglobin levels and
LDH isozyme compositions in the heart, gastrocnemius
and pectoralis muscles were determined in Common Murre
adults and during maturation of the chick at sea. Oxygen
stores in the chick (hemoglobin, hematocrit, muscle myoglobin)
increased significantly with growth. High levels of
the aerobic isozyme, LDH 1, were maintained throughout maturation
in the heart. All five LDH isozymes were maintained
at similar levels in the gastrocnemius muscle. The
pectoralis showed an increase in LDH 1, 2, 3, and 4, yet
retained relatively high levels of LDH 5 throughout maturation.
Upon leaving the nesting colony, metabolic
capacities in the heart and gastrocnemius of the chick are
similar to those of adults. The chick pectoralis tissue, however, gains aerobic capacities with maturation which is concomitant with the needed capacity for aerial and aquatic
flight upon fledging.
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Cytotoxicity and Effects on Cell Viability of Nickel NanowiresRodriguez, Jose E. 05 1900 (has links)
Recently, magnetic nanoparticles are finding an increased use in biomedical applications and research. Nanobeads are widely used for cell separation, biosensing and cancer therapy, among others. Due to their properties, nanowires (NWs) are gaining ground for similar applications and, as with all biomaterials, their cytotoxicity is an important factor to be considered before conducting biological studies with them. In this work, the cytotoxic effects of nickel NWs (Ni NWs) were investigated in terms of cell viability and damage to the cellular membrane.
Ni NWs with an average diameter of 30-34 nm were prepared by electrodeposition in nanoporous alumina templates. The templates were obtained by a two-step anodization process with oxalic acid on an aluminum substrate. Characterization of NWs was done using X-Ray diffraction (XRD) and energy dispersive X-Ray analysis (EDAX), whereas their morphology was observed with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell viability studies were carried out on human colorectal carcinoma cells HCT 116 by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cell proliferation colorimetric assay, whereas the lactate dehydrogenase (LDH) homogenous membrane fluorimetric assay was used to measure the degree of cell membrane rupture. The density of cell seeding was calculated to obtain a specific cell number and confluency before treatment with NWs. Optical readings of the cell-reduced MTT products were measured at 570 nm, whereas fluorescent LDH membrane leakage was recorded with an excitation wavelength of 525 nm and an emission wavelength of 580 - 640 nm.
The effects of NW length, cell exposure time, as well as NW:cell ratio, were evaluated through both cytotoxic assays. The results show that cell viability due to Ni NWs is affected depending on both exposure time and NW number. On the other hand, membrane rupture and leakage was only significant at later exposure times. Both cytotoxic assessment assays showed an earlier cytotoxic effect in case of shorter NWs, with longer ones having a more marked toxicity, albeit with a delay in time. These findings demonstrate that different levels of biocompatibility can be obtained with specific doses and properties of Ni NWs and can serve as guideline for future experiments.
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Determinação das atividades da ATP:creatina-fosfotransferase (E.C. 2.7.3.2) e da L-lactato:NAD-oxidorredutase (E.C. 1.1.1.27) e de suas isoenzimas em indivíduos portadores de neoplasias gástricas / Activities of the creatine kinase and lactate dehydrogenase isoenzymes in serum and tissues of patients with neoplasmsHirata, Rosario Dominguez Crespo 02 October 1987 (has links)
As atividades enzimáticas e isoenzimáticas da CK e da LD foram determinadas nos tecidos gástricos neoplásico e da margem de ressecção e no soro de indivíduos com adenocarcinoma de estômago, submetidos à gastrectomia. O tecido gástrico neoplásico aapresentou índices CKBB/Cktotal e LD5/LD1, bem como atividade da LD5, superiores aos da margem de ressecção correspondente. No pré-operatório, os indivíduos estudados apresentaram elevação da atividade sérica da CK BB (100%) e da CK MB (69%). Este fato, possivelmente, resultou da liberação dessas isoenzimas pelo próprio neoplasma ou, também pelo tecido normal adjacente. As atividades séricas das isoenzimas da LD apresentaram-se dentro dos valores de referência, nesse período. Após a gastrectomia, houve aumento significativo das atividades isoenzimáticas séricas da CK e da LD relação àquelas do pré-operatório, notadamente, no 1º período pós-operatório. Tais alterações foram atribuídas à liberação dessas isoenzimas pelos tecidos lesados durante o ato cirúrgico. / Abstract not available
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