Urban open spaces are of strategic importance to the quality of life of our increasingly urbanized society. Trees and related vegetation are planted and managed within the communities and cities to create or add value to the busy lives of the city dwellers. Trees in towns and cities form an important part of complex urban ecosystems and provide significant ecosystem services and benefits for urban dwellers, for example: reducing particulate pollution, carbon sequestration, decreasing air temperature, decreasing water runoff, aesthetic value and an increase in human health. Trees are solarpowered technology that can help restore balance to dysfunctional urban ecosystems. Trees form strands in the urban fabric that connect people to nature and to each other. The urban environment puts tremendous strain on trees by trenching, limited space for root growth and emission of pollutants into the atmosphere, water and soil. The problem is that the real impact of the urban environment on the trees within our community is unknown. The aim of this investigation was to assess the overall anthropogenic and environmental impacts on urban trees by measuring the tree vitality of Acacia karroo using chlorophyll fluorescence kinetics (JIP-test) and the leaf water potential using a pressure chamber. Tree vitality was quantified as the chlorophyll fluorescence-based performance index (PIABS)T. ree vitality measurements were also correlated with soil physical and chemical data. In the comparative study, an urbanization gradient approach was followed in which results of trees in rural areas were regarded as controls. The gradient approach is used worldwide and provides a background for questions of ecological structure and function. The urbanization gradient was quantified using the V-I-S model, based on % cover of vegetation, impervious surface and soil. Additionally, a model to determine the monetary value of trees in urban environments (SATAM) was tested. All this information could eventually contribute to develop an urban tree management program for Potchefstroom. It was evident from the current study that urbanization has a negative impact on tree vitality. The leaf water potential of a tree was, however, not necessarily negatively impacted upon. Although trees in urban environments did not always have a high vitality (PIABS)t, hey still played a major role in the urban environment. According to the tree appraisal method (SATAM), some of these trees have a value of R60 000. / Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

Urban open spaces are of strategic importance to the quality of life of our increasingly urbanized society. Trees and related vegetation are planted and managed within the communities and cities to create or add value to the busy lives of the city dwellers. Trees in towns and cities form an important part of complex urban ecosystems and provide significant ecosystem services and benefits for urban dwellers, for example: reducing particulate pollution, carbon sequestration, decreasing air temperature, decreasing water runoff, aesthetic value and an increase in human health. Trees are solarpowered technology that can help restore balance to dysfunctional urban ecosystems. Trees form strands in the urban fabric that connect people to nature and to each other. The urban environment puts tremendous strain on trees by trenching, limited space for root growth and emission of pollutants into the atmosphere, water and soil. The problem is that the real impact of the urban environment on the trees within our community is unknown. The aim of this investigation was to assess the overall anthropogenic and environmental impacts on urban trees by measuring the tree vitality of Acacia karroo using chlorophyll fluorescence kinetics (JIP-test) and the leaf water potential using a pressure chamber. Tree vitality was quantified as the chlorophyll fluorescence-based performance index (PIABS)T. ree vitality measurements were also correlated with soil physical and chemical data. In the comparative study, an urbanization gradient approach was followed in which results of trees in rural areas were regarded as controls. The gradient approach is used worldwide and provides a background for questions of ecological structure and function. The urbanization gradient was quantified using the V-I-S model, based on % cover of vegetation, impervious surface and soil. Additionally, a model to determine the monetary value of trees in urban environments (SATAM) was tested. All this information could eventually contribute to develop an urban tree management program for Potchefstroom. It was evident from the current study that urbanization has a negative impact on tree vitality. The leaf water potential of a tree was, however, not necessarily negatively impacted upon. Although trees in urban environments did not always have a high vitality (PIABS)t, hey still played a major role in the urban environment. According to the tree appraisal method (SATAM), some of these trees have a value of R60 000. / Thesis (M. Environmental Science (Ecological Remediation and Sustainable Utilisation))--North-West University, Potchefstroom Campus, 2007.

To decrease dependency of fossil fuels and avoid direct competition with food crops, massive research efforts are investigating next-generation cellulose biofuel crops such as switchgrass (Panicum virgatum). A low-input, sustainable switchgrass production could be achieved by reducing traditional management practices though applying plant growth promoting rhizobacteria (PGPR), of which our understanding is still rather limited. To elucidate physiological mechanisms behind PGPR's beneficial effects, we inoculated switchgrass seedlings with Burkholderia phytofirmans strain PsJN. Two experiments were conducted to determine the initial and long-term responses of switchgrass to PsJN inoculation by tracking growth and leaf physiology. In a third experiments, we tested the effects of PsJN on growth and leaf-level physiology of switchgrass under a moderate pre-drought conditioning and a successive severe drought stress. PsJN inoculation increased biomass and promoted elongation of shoots within 17 days following inoculation. The enhanced root growth in PsJN inoculated plants lagged behind the shoot response, resulting in greater allocation to aboveground growth (p=0.0041). Lower specific root length (p=0.0158) and higher specific leaf weight (p=0.0029) were also observed in PsJN inoculated seedlings, indicating advanced development. Photosynthetic rates (Ps) were higher in PsJN inoculated seedlings after 17 days (54%, p=0.0016), which were related to higher stomatal conductance, greater water use efficiency, and lower non-stomatal limitation of Ps. These rapid changes in leaf physiology are at least partially responsible for switchgrass growth enhancement from PsJN treatment. The early growth enhancement in PsJN inoculated switchgrass linearly decreased with plant age. PsJN inoculation increased Ps of upper canopy leaves by 13.6% but reduced Ps of lower canopy leaves by 8.2%. Accelerated leaf senescence and early flowering were observed in PsJN-inoculated switchgrass, which might contribute to slightly lower aboveground biomass at final harvesting. Drought preconditioning increased Ps of PsJN-inoculated switchgrass during a later severe drought; whereas, control switchgrass only benefited from drought preconditioning when leaf water potential dropped below -1 MPa. This study verified early growth enhancement and accelerated development of switchgrass due to PsJN inoculation. Rapid improvement in leaf physiology is related to enhanced productivity. PsJN inoculation also improve drought tolerance of switchgrass. / Ph. D.

O milho é uma das culturas de grande importância no cenário agrícola mundial devido sua importante participação no setor alimentício, bem como suas exigências hídricas no decorrer do seu ciclo. Sendo assim, tornam-se importantes os estudos quanto a utilização dos recursos hídricos, onde as estratégias de irrigação são de grande importância para a economia de água. O objetivo do presente estudo foi o de avaliar diferentes lâminas de irrigação (déficit e excesso) no decorrer do ciclo total e em fases fenológicas especificas da cultura do milho. Foram avaliadas as características de desenvolvimento, produção e estado hídrico da planta. Os experimentos foram realizados no Departamento de Engenharia de Biossistemas na Escola Superior de Agricultura \"Luiz de Queiroz\", ESALQ-USP, localizada em Piracicaba - SP. O delineamento experimental foi em blocos casualizados, com 12 tratamentos e quatro repetições. Os tratamentos consistiam na reposição de 150, 100, 50 e 30% da água evapotranspirada (ETc), aplicadas em quatro subperíodos do ciclo total da cultura: subperíodo 1 (V4 a V8), subperíodo 2 (V8 a Vp), subperíodo 3 (Vp a R1) e subperíodo 4 (R1 a R6). Os dados de cada experimento foram submetidos a análise de variância individual e quando possível, análise de variância conjunta, utilizando os dados médios dos experimentos. O 1° plantio foi realizado em 18 de maio de 2012 e o 2° 21 de abril de 2013 em ambiente protegido, totalizando 137 e 144 dias de ciclo, com soma-térmica de 1413 e 1444°C graus dias acumulados (GDA), respectivamente. Avaliou-se a altura das plantas (ALT), altura de inserção da espiga (AIT.I), número de espigas por planta (N.E), peso da espiga com palha (PEP), peso da espiga despalhada (PED), número de grão por espiga (NG), número de fileiras de grão por espiga (NFG), tamanho da espiga (TE), diâmetro da espiga (DE) diâmetro do sabugo (DS), número de entrenós (NE) e produtividade (PG). Mediu-se a temperatura foliar (Tf), déficit de pressão e vapor (DPV) e potencial hídrico foliar(-Ψf). O potencial hídrico foliar mostrou ser o método eficaz quanto ao estado hídrico da planta, havendo diferenciação entre os quatro tratamentos estudados (IIII, EEEE, D50 e D70), demonstrando variação do seu valor ao longo do dia. Calculou-se o coeficiente de resposta da cultura ao déficit hídrico (KY), onde o período de pendoamento e floração foram os mais críticos quando a deficiência hídrica foi imposta em fases fenológicas especificas. A partir dos dados de produtividade e lâminas de água aplicadas nos dois experimentos, foi possível ajustar modelos polinomiais de 2° grau para a produtividade da água (PA) e função de produção da cultura. Os resultados permitiram concluir que a maior PA foi obtida no tratamento D50. / Deficit and water excess in maize (Zea mays L.) in greenhouse Maize is a culture with importance in global scenario due your function in food industry, as well as yours water requirements in the course of your cycle. Therefore, studies became important regarding the use of water resources where irrigation strategies are of great importance for water savings. This study aimed evaluate different irrigation levels (deficit and water excess) during a total maize cycle and at different specifics phenological stages of the culture. Development characteristics, production and hydric state of the plant were evaluated. The experiments were conducted at Department of Biosystems Engineering from \"Luiz de Queiroz\" College of Agriculture, located at Piracicaba, São Paulo State. The experimental design was a randomized block, with 12 treatments and 4 repetitions. Treatments consisted in replacement of 150, 100, 50 and 30% of water evapotranspired (ETc), applied in four subperiods of the total culture cycle: subperiod 1 (V4 to V8), subperiod 2 (V8 to Vp), subperiod 3 (Vp to R1) and subperiod 4 (R1 to R6). The experimental data were submitted to individual variance analysis, and when were possible, conjunct variance analysis, using the average data of the experiments. The first planting was carried out on 18 May of 2012 and the second, on April 21 of 2013, in greenhouse, totaling 137 and 144-day cycle, with sum-Thermal 1413 and 1444 °C accumulated degree days (ADD),respectively. Were evaluated the plant height (PH); ear height insertion (AIT.I); number of ears per plant (NE); ear weight with straw (PEP); weight of ear without straw (PED); number of grain per ear (NG); number of grain rows per ear (NFG); ear size (TE); ear diameter (dE); diameter of the cob (DS); number of between-nodes (NE) and productivity (PG). The leaf temperature was measured (Tm), also the vapor pressure deficit (VPD) and leaf water potential (Pl). Leaf water potential showed to be an efficient method regarding hydric state of the plant, there were difference between the four treatments studied (III, EEEE, D50 e D70), demonstrating variation of its value over the day. the response coefficient of the crop to water deficit (KY) were calculated, where the period of bolting and flowering were the most critical when water stress was imposed in specific phenological stages. From the productivity data and water levels applied in both experiments, was possible adjust second degree polynomials models to water productivity (WP) and culture production function. The results allowed conclude that a higher WP were obtained in the treatment D50.

O milho é uma das culturas de grande importância no cenário agrícola mundial devido sua importante participação no setor alimentício, bem como suas exigências hídricas no decorrer do seu ciclo. Sendo assim, tornam-se importantes os estudos quanto a utilização dos recursos hídricos, onde as estratégias de irrigação são de grande importância para a economia de água. O objetivo do presente estudo foi o de avaliar diferentes lâminas de irrigação (déficit e excesso) no decorrer do ciclo total e em fases fenológicas especificas da cultura do milho. Foram avaliadas as características de desenvolvimento, produção e estado hídrico da planta. Os experimentos foram realizados no Departamento de Engenharia de Biossistemas na Escola Superior de Agricultura \"Luiz de Queiroz\", ESALQ-USP, localizada em Piracicaba - SP. O delineamento experimental foi em blocos casualizados, com 12 tratamentos e quatro repetições. Os tratamentos consistiam na reposição de 150, 100, 50 e 30% da água evapotranspirada (ETc), aplicadas em quatro subperíodos do ciclo total da cultura: subperíodo 1 (V4 a V8), subperíodo 2 (V8 a Vp), subperíodo 3 (Vp a R1) e subperíodo 4 (R1 a R6). Os dados de cada experimento foram submetidos a análise de variância individual e quando possível, análise de variância conjunta, utilizando os dados médios dos experimentos. O 1° plantio foi realizado em 18 de maio de 2012 e o 2° 21 de abril de 2013 em ambiente protegido, totalizando 137 e 144 dias de ciclo, com soma-térmica de 1413 e 1444°C graus dias acumulados (GDA), respectivamente. Avaliou-se a altura das plantas (ALT), altura de inserção da espiga (AIT.I), número de espigas por planta (N.E), peso da espiga com palha (PEP), peso da espiga despalhada (PED), número de grão por espiga (NG), número de fileiras de grão por espiga (NFG), tamanho da espiga (TE), diâmetro da espiga (DE) diâmetro do sabugo (DS), número de entrenós (NE) e produtividade (PG). Mediu-se a temperatura foliar (Tf), déficit de pressão e vapor (DPV) e potencial hídrico foliar(-Ψf). O potencial hídrico foliar mostrou ser o método eficaz quanto ao estado hídrico da planta, havendo diferenciação entre os quatro tratamentos estudados (IIII, EEEE, D50 e D70), demonstrando variação do seu valor ao longo do dia. Calculou-se o coeficiente de resposta da cultura ao déficit hídrico (KY), onde o período de pendoamento e floração foram os mais críticos quando a deficiência hídrica foi imposta em fases fenológicas especificas. A partir dos dados de produtividade e lâminas de água aplicadas nos dois experimentos, foi possível ajustar modelos polinomiais de 2° grau para a produtividade da água (PA) e função de produção da cultura. Os resultados permitiram concluir que a maior PA foi obtida no tratamento D50. / Deficit and water excess in maize (Zea mays L.) in greenhouse Maize is a culture with importance in global scenario due your function in food industry, as well as yours water requirements in the course of your cycle. Therefore, studies became important regarding the use of water resources where irrigation strategies are of great importance for water savings. This study aimed evaluate different irrigation levels (deficit and water excess) during a total maize cycle and at different specifics phenological stages of the culture. Development characteristics, production and hydric state of the plant were evaluated. The experiments were conducted at Department of Biosystems Engineering from \"Luiz de Queiroz\" College of Agriculture, located at Piracicaba, São Paulo State. The experimental design was a randomized block, with 12 treatments and 4 repetitions. Treatments consisted in replacement of 150, 100, 50 and 30% of water evapotranspired (ETc), applied in four subperiods of the total culture cycle: subperiod 1 (V4 to V8), subperiod 2 (V8 to Vp), subperiod 3 (Vp to R1) and subperiod 4 (R1 to R6). The experimental data were submitted to individual variance analysis, and when were possible, conjunct variance analysis, using the average data of the experiments. The first planting was carried out on 18 May of 2012 and the second, on April 21 of 2013, in greenhouse, totaling 137 and 144-day cycle, with sum-Thermal 1413 and 1444 °C accumulated degree days (ADD),respectively. Were evaluated the plant height (PH); ear height insertion (AIT.I); number of ears per plant (NE); ear weight with straw (PEP); weight of ear without straw (PED); number of grain per ear (NG); number of grain rows per ear (NFG); ear size (TE); ear diameter (dE); diameter of the cob (DS); number of between-nodes (NE) and productivity (PG). The leaf temperature was measured (Tm), also the vapor pressure deficit (VPD) and leaf water potential (Pl). Leaf water potential showed to be an efficient method regarding hydric state of the plant, there were difference between the four treatments studied (III, EEEE, D50 e D70), demonstrating variation of its value over the day. the response coefficient of the crop to water deficit (KY) were calculated, where the period of bolting and flowering were the most critical when water stress was imposed in specific phenological stages. From the productivity data and water levels applied in both experiments, was possible adjust second degree polynomials models to water productivity (WP) and culture production function. The results allowed conclude that a higher WP were obtained in the treatment D50.

Submitted by (edna.saturno@ufrpe.br) on 2016-06-16T15:38:58Z No. of bitstreams: 1 Elizamar Ciriaco da Silva.pdf: 1863733 bytes, checksum: ec224eb207a54d96ab1d9a11eceb2f54 (MD5) / Made available in DSpace on 2016-06-16T15:38:58Z (GMT). No. of bitstreams: 1 Elizamar Ciriaco da Silva.pdf: 1863733 bytes, checksum: ec224eb207a54d96ab1d9a11eceb2f54 (MD5) Previous issue date: 2008-02-22 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Among the principal native fruit trees in Northeastern Brazil, especially those found in the semi-arid areas, the umbu tree (Spondias tuberosa Arruda) represents itself as an important alternative as it well accepted by consumers and is a good produce in dry environments.Thus, the fruit trade fair or through cooperatives provides a source of supplementary income for small farmers. However, this income can be compromised by harvesting and excessive deforestation, which has intensified each year. Concern with population reduction of this species and by anthropic, Brazilian Institute for the Semi-Arid Tropic has developed studies on seedlings production, cultivation, and genetic inheritance preservation recovering genotypes with distinct morphological characteristics and deployment of a germplasm active bank provide the most promising for small producers, in addition to contributing to the reforestation of the Caatinga with a native species. Of the climatic factors limiting fruit species production in the semi-arid northeast, drought is the main factor, also allied to the growing problem of soil salinization, which has worsened each year. The mechanisms used by umbu tree to tolerate drought is not well elucidated and the physiological response before soil salinity is not yet known. Thus, the present work aimed to evaluate the physiological responses of umbu tree to drought and salt stresses. To evaluate drought responses, a project was developed in green house conditions using four grafted genotypes classified as giantumbu (BGU 44, BGU 48, BGU 50 and BGU 68) in order to evaluate the alterations on stomatal behavior, anatomical parameters, water relations and some biochemical aspects induced by intermittent drought and the possible genotypical variations. Transpiration (E) and diffusive resistance (rs) were measured daily after the beginning of the stress treatments by withholding water. When plants presented stomatal closure, the vases were re-watered and the water withhold again. This cycle was repeated for a 31 period days. The leaf water potential (Yw) was measured in four-hour intervals during a 24-hour period at the moment of the first stomatal closure and at the end of the experimental period. Total soluble carbohydrates (CHS), free amino acids (AA), protein (PROT) and proline (PRO) in leaves and roots were also measured. Certain regularity in the stomatal closure was observed among the watering period, showing differences between the species. The correlation with environmental factors suggest that, besides the water, stomatal behavior of BGU 44 and BGU 68 were influenced by Tar, RH and VPD, while the access BGU 50 were influenced by PAR and BGU 48 had no correlation with these environmental factors, suggesting that the water exerted the major influence in this genotype. Anatomical alterations in response to drought on stomatal density (DE) and reductions on stomatal index (IE) and stomatal aperture size (AO) were observed.The access BGU 48 maintained its anatomical features unaltered. There was an inversion in tissue proportion in BGU 44 under stress conditions, reducing the thickness of the spongy parenchyma and increasing palisade parenchyma. The inverse occurred with BGU 68 and theremaining genotypes continued unchanged. The lower Yw time of most of the genotypes was between 8h and 12h. The Yw of the stressed plants of BGU 44 and BGU 50 reduced significantly at 8h. The highest Yw was observed to BGU 68. The stress prolongation induced reductions in CHS content in the leaves of all genotypes. There were increases in the leaves to AA in BGUs 44 and 48, while BGUs 50 and 68 were reduced by about 40% and 43% respectively. BGU 44 and BGU 50 kept this behavior at the end of the experimental period.Significant differences in PROT content were not observed, but there were increases of 50% in PRO, except to BGU 50. Alterations on CHS, AA and PRO contents in the roots were verified and varied among the different genotypes. BGU 68 and BGU 50 were the most contrasting genotypes. In order to evaluate the salt stress responses in umbu plants a project was developed using seedlings propagated by seeds. Plants were grown in washed sand with Hoagland & Arnon nutrient solution without salt and with 25, 50, 75 and 100mM NaCl. Growth, Yw, E and rs were then evaluated. Na+, K+, Cl-, soluble carbohydrates and free amino acid contents were measured in several plant organs. Most variables were affected with salinity above 50 mM NaCl showing decreases in: number of leaves, plant height, stems diameter and dry masses and increases in root to shoot ratio. Reductions in pre-dawn leafwater potential (Ypd) were observed in plants submitted to 75 and 100 mM NaCl. Salt levels applied increased Na+ and Cl- contents in leaves. However, K+ content was not affected. A saturation to retain Na+ and Cl- in stems and roots was verified in treatments above 50 mM NaCl. These results allow us to say that there are physiological and anatomical differences among umbu tree genotypes; genotypes respond differently to intermittent drought; the turgor maintenance in umbu tree is relative to water storage in the xylopodium associated with the efficient stomatal closure mechanism and not by osmotic active solutes accumulation in either drought or salt stress conditions; due to the great variation found, the organic solutes accumulations did not demonstrate to be a good physiological trait as indicator to droughtand salt-tolerance in umbu plants. This specie tolerates salt levels until 50 mM NaCl withoutshowing significant physio-morphological alterations. / Dentre as principais fruteiras nativas do Nordeste, especialmente aquelas encontradas no semi-árido, o umbuzeiro (Spondias tuberosa Arruda) desponta com uma alternativa importante, por ser uma fruta bem aceita pelo consumidor e por ter uma boa produção em ambientes secos. Dessa forma, o comércio dos frutos em feiras livres ou através de cooperativas proporciona uma fonte de renda complementar para os pequenos agricultores. No entanto, essa renda pode ser comprometida pelo extrativismo e o desmatamento excessivos, que tem se intensificado a cada ano. Preocupada com a redução populacional desta espécie pela ação antrópica, a Embrapa Semi-Árido vem desenvolvendo estudos sobre produção de mudas, cultivo e preservação da herança genética, através da recuperação de acessos com características morfológicas distintas e a implantação de um banco ativo de germoplasma, para disponibilizar os mais promissores para os pequenos agricultores, além de contribuir com o reflorestamento da Caatinga com uma espécie nativa. Dos fatores climáticoslimitantes na produção de espécies frutíferas no semi-árido nordestino, a seca é o principal fator, aliado também ao problema crescente de salinização dos solos, que tem se agravado a cada ano. Os mecanismos utilizados pelo umbuzeiro paratolerar a seca ainda não estão completamente esclarecidos e não se conhece ainda as respostas fisiológicas frente a salinidade do solo. Desta forma, o presente trabalho objetivou avaliar as respostas fisiológicasdo umbuzeiro quando submetido às condições de seca e salinidade. Para avaliar as respostas à seca, desenvolveu-se um experimento em casa de vegetação utilizando mudas enxertadas de quatro acessos de umbuzeiro (acessos BGU 44, BGU 48, BGU 50 e BGU 68) classificados como umbu-gigante, com o objetivo de avaliar as alterações no comportamento estomático, parâmetros anatômicos, relações hídricas e alguns parâmetros bioquímicos induzidos pela seca intermitente, além das possíveis variações genotípicas. Foram efetuadas mensurações da transpiração (E) e da resistência difusiva (rs) diariamente após a suspensão da rega até ocorrer o fechamento estomático, momento em que as plantas foram re-irrigadas. A rega foi suspensa novamente até ocorrer novo fechamento estomático e este ciclo foi repetido por um período de 31 dias. O potencial hídrico foliar (Yw) foi determinado em dois cursos nictimerais (no momento do primeiro fechamento estomático e ao final do período experimental). Também foram avaliados os teores de carboidratos solúveis totais (CHS), aminoácidos livres (AA), proteína (PROT) e prolina (PRO) nas folhas e nas raízes, assim como alterações anatômicas. Os acessos apresentaram regularidade no período de fechamento estomático entre as regas, demonstrando diferenças intra-específicas. Houve correlação com as variáveis ambientais sugerindo que, além da água, o comportamento estomático dos acessos BGU 44 e BGU 68sofreram influência da Tar, UR e DPV, enquanto que o acesso BGU 50 sofreu influência do PAR e o BGU 48 não se correlacionou com os outros fatores, indicando que a água foi o fator que exerceu maior influência neste acesso. Alterações anatômicas em resposta à seca foramobservadas na densidade de estômatos (DE), reduções no índice estomático (IE) e na abertura do ostíolo (AO). O acesso BGU 48 manteve as características anatômicas inalteradas. Houve uma inversão na proporção dos tecidos do acesso BGU 44 quando sob estresse, diminuindo a espessura do parênquima lacunoso e aumentando o parênquima paliçádico. O inverso ocorreu com o BGU 68 e os demais acessos permaneceram inalterados. O horário de menor Yw para a maioria dos acessos foi entre 8h e 12h. O Yw das plantas estressadas do BGU 44 e BGU 50 foi reduzido significativamente às 8h. O BGU 68 apresentou os valores mais elevados de Yw. O prolongamento do estresse provocou reduções nos teores de CHS nas folhas de todos os acessos. Houve aumento no teor de AA nas folhas dos BGU’s 44 e 48, enquanto que os BGU’s 50 e 68 reduziram 40% e 43%, respectivamente. Ao final do período experimental esse comportamento se manteve para o BGU 44 e o BGU 50. Não houve diferença significativa para os teores de PROT nas folhas, mas houve aumento de 50% nos teores de PRO, exceto para o BGU 50. Foram verificadas alterações na concentração de CHS, AA e PRO nas raízes, com diferença entre os acessos. Os acessos BGU 68 e BGU 50 foram os mais contrastantes em condições de seca. Para avaliar as respostas do umbuzeiro ao estresse salino, foi desenvolvido um experimento utilizando-se plantas propagadas por sementes. As plantas foram cultivadas em areia lavada, regadas com solução nutritiva de Hoagland & Arnon, sem ecom adição de NaCl (25, 50, 75 e 100 mM). Avaliou-se o crescimento, o Yw, E e rs. O teor de Na+, K+, Cl-, carboidratos solúveis e aminoácidos livres foram dosados nos diversos órgãos da planta. A maioria das variáveis estudadas foi afetada em níveis de NaCl de 50 mM, reduzindo o número de folhas, a altura das plantas, o diâmetro do caule e a massa seca e aumentando a relação raiz/parte aérea (R/Pa). O potencial hídrico foliar antes do amanhecer (Ypd) foi reduzido nas plantas dos tratamentos 75 e 100 mM de NaCl. A concentração de Na+ e Cl- nas folhas aumentou em função dos níveis de NaCl aplicados, mas, o teor de K+ não foi afetado. Nos caules e raízes, houve uma saturação na retenção de Na+ e Cl- nos tratamentos acima de 50 mM. Os resultados desta pesquisa permite inferir que existem diferenças fisiológicas e anatômicas entre os acessos de umbuzeiro estudados; que eles respondem de forma diferente à seca intermitente; que a manutenção da turgescência foliar está relacionada à reserva de água nos xilopódios associado ao mecanismo de fechamento estomático eficiente e não ao acúmulo de solutos osmoticamente ativos, tanto em situação de seca como de salinidade no meio; devido à grande variação encontrada, o acúmulo de solutos orgânicos não demonstrou ser um mecanismo fisiológico indicador de tolerância à seca e a salinidade nesta espécie; o umbuzeiro tolera níveis de salinidade de até 50 mM de NaCl sem apresentar alteraçõesfisiomorfológicas significativas na fase inicial do desenvolvimento.

La zone sahélienne a connu une baisse des précipitations et d’importants changements d'utilisation des terres ces dernières décennies qui ont conduit à une forte régression du couvert ligneux. Pourtant, Guiera senegalesis J.F. Gmel est une Combretaceae semi-sempervirente, dont la dominance s'est accrue avec la pression agricole dans la bande soudano-sahélienne où elle joue un rôle socio-économique et agroforestier important. Elle constitue donc un bon modèle pour étudier les réponses hydriques du couvert ligneux actuel à la sécheresse et les modifications de ces réponses sous l'effet de la coupe.Les objectifs de ce travail de thèse étaient : 1) de caractériser et quantifier les processus de régulation hydrique chez G. senegalensis et de déterminer ses limites fonctionnelles, afin d’estimer sa marge de sécurité dans ses conditions actuelles de croissance, 2) d’estimer l’effet de la coupe annuelle sur ses processus de régulation et 3) d’analyser les variations de ses traits foliaires en fonction de la saison et des conditions de croissance.Les mesures et les observations ont été réalisées d’avril 2009 à février 2011 sur une jachère âgée, sur une jeune jachère et sur deux champs de mil adjacents aux deux jachères respectivement. A l'échelle de l'arbuste, le potentiel hydrique foliaire et la conductance stomatique ont été mesurés au cours de ces deux saisons de croissance sur un échantillon d'arbustes dans les trois types de couvert. Le taux de transpiration foliaire et la conductance hydraulique sol-feuille en ont été déduits. A l’échelle du rameau, ont été suivi la dynamique du nombre de feuilles, de la surface moyenne des feuilles, du diamètre moyen des tiges qui les portent. La durée de vie moyenne des feuilles et leur masse surfacique ont été mesurées. La courbe de vulnérabilité à la cavitation de l'espèce, qui est la perte de la conductivité hydraulique en fonction du potentiel hydrique, a été réalisée à partir de plusieurs segments de branches passés au CAVITRON en laboratoire.Les principaux résultats obtenus sont que la fermeture des stomates qui permet à l’espèce de réguler ses pertes en eau a lieu à un niveau faible de cavitation du xylème (30%). Ainsi, l’espèce peut survivre dans les conditions sahéliennes grâce à une « marge de sécurité » positive de +0,6 MPa. La régulation stomatique couplée à un ajustement foliaire permet à l’espèce de maintenir son équilibre hydrique au cours de la saison sèche chez les arbustes matures non perturbés. La baisse des potentiels hydriques foliaires de base et minimum en réponse à la sécheresse saisonnière est isohydrodynamique et est plus élevé chez les arbustes matures, suggérant que les repousses de l'année sont moins stressées. De plus, la coupe a pour effet de découpler l’ajustement foliaire de la régulation stomatique et entraîne une production de feuilles et une croissance des jeunes rameaux continues tout au long de l'année chez les repousses, grâce à une conductance hydraulique sol-feuille deux fois plus élevée que chez les arbustes matures. Enfin, la durée de vie des feuilles s’allonge avec l’âge des rameaux d'une année comme avec l'âge du peuplement, tandis que la masse surfacique foliaire augmente progressivement de la même façon quand on passe de la saison des pluies à la saison sèche, quel que soit l'âge du peuplement.Ces traits fonctionnels contribuent à ce que l’espèce puisse à la fois résister aux conditions de sécheresse dans lesquelles elle croît et ré-allouer de façon intense des ressources, stockées probablement dans les racines, à la reconstruction rapide de sa partie aérienne à la suite d'une coupe. Les limites d'une telle stratégie restent cependant à identifier, en particulier le seuil d'épuisement des ressources sous l'effet de la répétition annuelle des coupes. / Sahel suffered from severe rainfall decreases combined to great changes in land use during the last decades that led to strong decrease in the woody cover. However, Guiera senegalesis J.F. Gmel is a semi-evergreen Combretaceae whose dominance rose with crop pressure in the Sudano-Sahelian band where it plays an important socio-economic role through agroforestry. Consequently, this is a good model to study hydric responses of the present woody cover to drought and the effect of cutting on these responses.The aims of this work were: 1) to characterize and quantify hydric regulation processes in G. senegalesis and to determine its specific functional limits, in order to estimate its safety margin in its current growth conditions, 2) to estimate cut effect on these processes and 3) to analyze variations of its leaf traits according to the season and its specific growth conditions.Measurements and observations were conducted from April 2009 to February 2011 on an old fallow, on a young fallow, and on the two crop fields adjacent to the two fallows respectively. At the shrub scale, leaf water potential and stomatal conductance were measured during the two growing seasons on shrub samples in the three cover type. The leaf transpiration rate and the soil-to-leaf conductivity were deduced. At the stem scale, the dynamics of the number of leaves, the mean leaf area (LMA), the mean diameter of the stems that carry them, the mean leaf life span, and the leaf mass per area were monitored. The species vulnerability curve to cavitation, which is the loss of conductivity as a function of the water potential, was obtained from several peaces of branches treated in a CAVITRON at the laboratory.The main results obtained were that the stomatal closure that allows species to regulate its water loss occurs at a low rate of xylem cavitation (30%). Thus, the species can survive in its Sahelian conditions thanks to a positive “safety margin” of +0.6 MPa. Stomatal regulation coupled with leaf adjustment allows the species to maintain its water balance during the dry season in mature and undisturbed shrubs. The decrease in predawn and midday leaf water potentials in response to seasonal drought was isohydrodynamic, and it was greater in mature shrubs, suggesting that current year resprouts are under less stress. In addition, cutting decouple leaf adjustment from stomatal regulation, leading to continuous leaf production and young stem growth all over the year, thanks to a soil-to-leaf conductivity seven times higher in the current year's resprouts than in the mature shrubs. Finally, leaf life span increases with the current year's stem age as well as with the stand age, while the LMA progressively increases from the rainy season to the dry season, similarly whatever the stand age.These functional traits contribute to the species resistance to the drought conditions under which it grows, but also, following the cutting, to intensively re-allocate resources, probably from the root reserve, to the fast re-building of the aerial part. However, the limits of such a strategy remain to be identified, especially the resource exhaustion threshold under repetitive yearly cutting.

Submitted by (ana.araujo@ufrpe.br) on 2017-02-17T12:38:37Z No. of bitstreams: 1 Jacqueline Wanessa de Lima Pereira.pdf: 684135 bytes, checksum: dc0f8da93d2685268558a2dc803e4044 (MD5) / Made available in DSpace on 2017-02-17T12:38:37Z (GMT). No. of bitstreams: 1 Jacqueline Wanessa de Lima Pereira.pdf: 684135 bytes, checksum: dc0f8da93d2685268558a2dc803e4044 (MD5) Previous issue date: 2010-07-07 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Peanut is one of the most cultivated oily in the world. For countries of semi-arid climate, as Brazil, for example, the peanut is an important alternative to farming due to the low water requirement during the cycle and adaptation to environments of high temperature and solar radiation. Despite the tolerance to low water availability, peanuts’ requirements are not the same over the cycle. Water shortage during the reproductive phase directly affects the formation and filling of pods, even in cultivars notoriously resistant to water deficit. According to some authors, which makes the peanut plant tolerant to environmental adversities are the morphological and physiological mechanisms that maintain the plant turgidity, even under low water availability conditions. Among those are cited changes in water ratios (stomatal behavior and osmotic adjustment) and the expansion of the root system for deeper and wetter soil areas. Faced with this adaptability, understanding the role of water deficit during the production cycle of culture is essential for adopting management strategies that enable secure production in areas prone to drought. In this work, four peanut genotypes of different growth habits were subjected to 21 days of water stress in a greenhouse. The planting was performed in pots containing sandy-loam texture soil, previously limed and fertilized. The experimental design was randomized with a bi-factorial 4x2 scheme (4 genotypes x 2 water treatments) with 10 repetitions. the water treatment were control (daily irrigation) and stress (irrigation suspension). The variables evaluated were difusive resistance, transpiration, leaf water potential, relative water content, proline content, chlorophyll content, root length, dry weight of pods, harvest index and stress tolerance index (STI). Under stress conditions, stomatal behavior was changed from the second week, when all genotypes significantly increased abaxial surface diffusive resistance and reduced sweating, highlighting the isoline LBM Branco Moita/08. The water potential of all genotypes was significantly reduced, being more expressive in isolines LBM-Branco Moita/08 and LBR-Branco Rasteiro/08 reaching more negative values. The LBM-Branco Moita/08 also had higher levels of proline and along with a BR 1 had the greatest expansion of the root system as adaptive way. In the production aspect, the LBM-Branco Moita/08 revealed the lowest reductions in weight of pods and harvest index when subjected to water deficit. Regarding the STI, values obtained with the cv. BR1 confirm its suitability for management in semi-arid environments; the strains LBR-Branco Rasteiro/08 and LBM-Branco Moita/08 also showed significant production performance in environments with water restriction. / O amendoim é uma das oleaginosas mais cultivadas no mundo. Para os países de clima semi-árido, o Brasil, por exemplo, o amendoim é uma importante alternativa para a agricultura devido à baixa exigência hídrica durante o ciclo e adaptação aos ambientes com altas temperaturas e radiação solar. Apesar da tolerância à baixa disponibilidade hídrica, as exigências do amendoim não são as mesmas ao longo do ciclo. A escassez de água durante a fase reprodutiva afeta diretamente a formação e o enchimento das vagens, mesmo em cultivares notoriamente resistentes ao déficit hídrico. Segundo alguns autores, o que torna a planta do amendoim tolerante às adversidades ambientais são os mecanismos morfológicos e fisiológicos que mantêm a turgescência das plantas, mesmo sob condições de baixa disponibilidade de água. Entre as mudanças já estão em relações hídricas (comportamento estomático e ajustamento osmótico) e a expansão do sistema radicular para as áreas mais profundas e úmidas do solo. Confrontados com esta capacidade de adaptação, a compreensão do papel do déficit hídrico durante o ciclo produtivo da cultura é essencial para a adoção de estratégias de manejo que permitam uma produção segura em áreas propensas à seca. Neste trabalho, quatro genótipos de amendoim de diferentes hábitos de crescimento foram submetidos a 21 dias de estresse hídrico em casa de vegetação. O plantio foi realizado em vasos contendo solo de textura franco-arenosa, previamente corrigido e adubado. O delineamento experimental foi inteiramente casualizado, com um esquema bi-fatorial 4x2 (4 genótipos x 2 tratamentos de água), com 10 repetições. Os tratamentos hídricos foram: controle (rega diária) e estresse (suspensão de rega). As variáveis avaliadas foram: resistência difusiva, transpiração, potencial hídrico, teor relativo de água, teor de prolina, teor de clorofila, comprimento radicular, peso seco das vagens, índice de colheita e índice de tolerância ao stress (STI). Sob condições de estresse, comportamento estomático foi alterado a partir da segunda semana, quando todos os genótipos aumentaram significativamente a resistência difusiva da superfície abaxial e reduziram a transpiração, destacando a isolinha LBM-Branco Moita/08. O potencial de água de todos os genótipos foi significativamente reduzido, sendo mais expressivos em isolinhas LBM-Branco Moita/08 e LBR-Branco Rasteiro/08 atingindo valores mais negativos. A LBM-Branco Moita/08 também apresentou elevado acúmulo de prolina e, juntamente com um BR 1 teve a maior expansão do sistema radicular como forma de adaptação. No aspecto de produção, a LBM-Branco Moita/08 revelou as menores reduções no peso das vagens e índice de colheita, quando submetida ao déficit hídrico. Quanto ao STI, os valores obtidos com a cv. BR1 confirmar a sua aptidão para o manejo em ambientes semi-árido, as isolinhas LBR-Branco Rasteiro/08 e LBM-Branco Moita/08 também apresentaram desempenho significativo de produção em ambientes com restrição hídrica.

Made available in DSpace on 2016-12-23T14:04:32Z (GMT). No. of bitstreams: 1 dissertacao_chris.pdf: 2110899 bytes, checksum: 589457df1d7b33218ca52d383800ff09 (MD5) Previous issue date: 2006-02-24 / The objective of this work was to evaluate the influence of the soil humidity in the field capacity in the tensions of 0,006 MPa (FC1), 0,010 MPa (FC2) and 0,033 MPa (FC3) and of different levels of water deficits (WD 0%, WD 33% and WD 67%) in the initial growth of the coffee plant conilon and in the leaf water potential measured in the anti-morning, in a Red-Yellow Oxisol (OR) and Red-Yellow Ultisol (URY). The experiment was let vegetation home, being cultivated the plants in vases of 12 liters during 255 days. The adopted experimental design was randomized entirely, mounted in outline of subdivided portions, with three repetitions for each soil. The growth evaluations were achieved each 60 days and the analyzed data by the surface technique of answering. The tenor of soil humidity in the field capacity varies with the adopted tension in its determination. The growth of the coffee plant conilon in WD 0% was higher to the obtained in the water deficits of 33% and 67% of OR and URY. The largest growth of the culture was observed in FC2 of OR and in FC1 of URY. The smallest growth was obtained in the water deficits of the certain field capacity in the tension of 0,033 MPa (FC3) of OR and URY, what unfeasible its adoption in the estimate of the irrigation sheet, being used the camera of pressure of Richards. The x leaf water potential anti-morning (Ψam) showed to be a good indicator of the degree of hydration of the plants. The largest hydration to foliate was observed in WD 0%, being in CC2 for OR (Ψam = -0,17 MPa), being the water kept by the soil to a potential matric (Ψm) of -0,010 MPa and in CC1 for URY (Ψam = -0,33 MPa), which Ψm was the -0,006 MPa. To smallest hydration happened in WD 67% and in the FC3, also for OR (Ψam = -0,68 MPa) as for URY (Ψam = -1,3 MPa), being the water kept of Ψm of -0,20 MPa, for both soils. In WD 33% and WD 67% in the levels of the capacity of field of OR and URY, they were verified a reduction in the values leaf area, height and diameter of the stem of the plants. The accumulation of total dry matter and leaf water potential anti-morning observed in OR were superior to the of URY, in all of the levels of the field capacity and water deficits, resulting in the larger initial growth of the coffee plant conilon in this soil. / O objetivo deste trabalho foi avaliar a influência da umidade do solo na capacidade de campo determinada nas tensões de 0,006 MPa (CC1), 0,010 MPa (CC2) e 0,033 MPa (CC3) e de diferentes níveis de déficits hídricos (DH 0%, DH 33% e DH 67%), no crescimento inicial do cafeeiro conilon e no potencial hídrico foliar medido na antemanhã, em um Latossolo Vermelho-Amarelo (LV) e Argissolo Vermelho-Amarelo (PVA). O experimento foi conduzido em casa de vegetação, cultivando-se as plantas em vasos de 12 litros durante 255 dias. O delineamento experimental adotado foi inteiramente casualizado, distribuído em esquema de parcelas subdivididas, com três repetições para cada solo. As avaliações de crescimento foram realizadas a cada 60 dias e os dados analisados pela técnica de superfície de resposta. O teor de umidade do solo na capacidade de campo varia com a tensão adotada em sua determinação. O crescimento do cafeeiro conilon em DH 0% foi superior aos obtidos nos déficits hídricos de 33% e 67% do LV e do PVA. O maior crescimento da cultura foi observado na CC2 do LV e na CC1 do PVA. O menor crescimento foi obtido nos déficits hídricos da capacidade de campo determinada na tensão de 0,003 MPa (CC3) do LV e do PVA, o que inviabiliza a sua viii adoção na estimativa da lâmina de irrigação, utilizando-se a câmara de pressão de Richards. O potencial hídrico foliar antemanhã (Ψam) mostrou-se bom indicador do grau de hidratação das plantas. A maior hidratação foliar foi observada em DH 0%, sendo para o LV na CC2 (Ψam= -0,17 MPa), estando a água retida pelo solo a um potencial matricial (Ψm) de -0,010 MPa, e para o PVA na CC1 (Ψam = -0,33 MPa), cujo Ψm foi de -0,006 MPa. A menor hidratação ocorreu em DH 67% na CC3 tanto para o LV (Ψam = -0,68 MPa) como para o PVA (Ψam = -1,30 MPa), estando a água retida a um Ψm de -0,20 MPa, para ambos os solos. Em DH 33% e DH 67% nos níveis de capacidade de campo do LV e do PVA, foram verificadas reduções nos valores da área foliar, altura e diâmetro do caule das plantas. O acúmulo de matéria seca total e potencial hídrico foliar antemanhã observados no LV foram superiores aos do PVA, em todos os níveis de capacidade de campo e déficits hídricos, resultando em maior crescimento inicial do cafeeiro conilon, neste solo.