PhD (Food Science) / Department of Food Science and Technology / Finger millet (FM) [Eleusine coracana] is an underutilised cereal grain used as a food source in South Africa. Increased research interest in FM has span over the years owing to its unique nutritional and bioactive composition. Following the recent interest in natural curative substances over their synthetic counterparts in the treatment of food dependent diseases, FM has shown potential nutraceutical effects. Some important health effects like antidiabetic, antioxidative, anti-inflammatory and antimicrobial properties have been reported in recent trials with FM. In view of the increasing utilisation and application of FM in the region of Thulamela Municipality, Vhembe District of South Africa, two common indigenous FM varieties (brown and dark brown) were obtained and analysed for their physicochemical properties, levels of minerals, phytic acid, phenolic compounds and antioxidant activities. For this process, malted non-alcoholic beverages were produced and analysed for their physicochemical properties, levels of phenolic compounds, and total phenolics and antioxidant activities. FM grains were soaked, germinated and kilned at an interval of 24 h for 96 h, using sorghum as an external reference. Mineral composition of the FM and sorghum samples were analysed using an inductively coupled plasma atomic emission spectroscopy (ICP-AES) and mass spectroscopy (ICP-MS), and atomic absorption spectrometer (AAS). Identification and quantification of phenolic compounds were performed using ultra-performance liquid chromatography mass spectrometer (UPLC-MS). All experiments were performed in triplicate except for the UPLC-MS analysis of the malted non-alcoholic beverages that was done in duplicate. Data were analysed by one way analysis of variance, and the mean values were separated by Duncan’s multiple comparison test using SPSS version 24.0. Data showed that the FM varieties were rich in macro- and micro- or trace elements. The macro-elements calcium, magnesium, potassium, phosphorus and sulphur were found in high amounts ranging from 1597.37 mg/ kg – 6775.03 mg/ kg; iron, zinc, strontium and silicon were found in significant amounts in the range 21.47 mg/ kg – 55.67 iii mg/ kg, copper and boron were found in low amounts (2.2 mg/ kg – 7.7 mg/ kg), along with selenium and cobalt (0.02 mg/ kg – 0.05 mg/ kg). Heavy metals, barium and aluminium were found in the FM varieties. Varietal difference was found to play an important role in the mineral content of the grains during malting. Malting for 24 h reduced mineral content except for sodium. Beyond 48 h of malting, mineral content increased, particularly, for 96 h in FM grain malt. Significant (p < 0.05) increases in the mineral content of FM varieties were noted at 48 h and 96 h of malting. Increase occurred at 72 h of malting for potassium, iron and boron. Malting did not have any effect on the manganese content of the dark brown FM; however, it increased the manganese content at 48 h of malting for brown FM. Malting for 96 h significantly (p < 0.05) reduced sodium content. Consecutive decrease in phytic acid content of the grains was not recorded with durations in malting time. Although statistically significant differences (p < 0.05) were observed, malting did not result in too much change in the physicochemical properties of the grains. Several flavonoids, catechin, epicatechin, quercetin, taxifolin, and hesperitin were isolated, whilst protocatechuic acid was the only phenolic acid detected in the unmalted and malted FM. Increases in catechin, epicatechin and protocatechuic acid were observed for 72 and 96 h malt of brown FM with similar observations recorded for sorghum. Complete loss of taxifolin, catechin, and hesperitin were noted with malting time. FM grains exhibited 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2՛-azinobis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical scavenging action and iron reducing activities. Increased iron reducing activity alongside ABTS radical scavenging activity was recorded with malting time. A fermentation-time dependent decrease in the pH of the non-alcoholic beverages, with a corresponding increase in sugar content were recorded. A similar decrease was also recorded for the viscosities of the beverages. The FM malt beverages were found to contain a higher amount of citric acid compared to the sorghum malt beverage. A decrease in the citric acid content with fermentation time was noted in the grain malt beverages fermented with Lactobacillus fermentum, particularly for the iv FM beverage. The phenolic compounds detected in the FM malt beverages fermented with the grain microbial flora and Lactobacillus fermentum were protocatechuic acid, catechin and epicatechin. Taxifolin and kaempferol along with the earlier mentioned compounds were detected in the sorghum malt beverage. Catechin was found in higher amount compared to other phenolic compounds in the FM and sorghum malt beverages. FM malt beverages were found to contain a higher amount of total phenolics compared to the beverage prepared from sorghum malt. Fermentation with the grains’ microbial flora and L. fermentum resulted in reduced total phenolics of FM and sorghum malt beverages, particularly after 24 h of fermentation. A fermentation-time dependent decrease in total phenolics of FM beverages fermented with L. fermentum was noted. Fermentation within 24 - 48 h using the grain microbial flora showed higher total individual phenolic compounds for the dark brown FM and sorghum, compared to other fermentation periods. Fermentation of the beverages for 24 h retained a higher amount of the total phenolics compared to other fermentation periods, especially for the L. fermentum beverages. Reduced total phenolic content and antioxidant activity of the beverages were noted at 24 h of fermentation for the two microbial sources. Significant (p < 0.05) increases in total phenolics were observed within 72 – 96 h of fermentation of the brown FM malt beverage with the grains’ microbial flora. Fermentation for 72 h and 96 h with L. fermentum increased the total phenolic content of the brown FM. Increase in total flavonoid content (TFC) of brown FM malt beverage was noted at 72 h fermentation for both microbial sources. Unlike with L. fermentum, no significant (p > 0.05) change in TFC was observed for the dark brown FM beverage after 24 h fermentation with the grains’ microbial flora. Beverages exhibited DPPH, ABTS radical scavenging action and iron reducing activities, which were significantly (p < 0.05) reduced at 96 h fermentation for both microbial sources. The 24 h fermented beverage retained a higher amount of total phenolic and flavonoid contents, and had higher antioxidant activity compared to other fermentation periods for both microbial sources. The study shows that FM is a rich source of essential minerals and v phenolic compounds, and demonstrates that 72 to 96 h of malting has a positive effect on minerals and certain phenolic compounds over the 48 h malting period widely used for preparation of FM malt. The presence of hesperitin in FM grain was established. A new method was developed for the production of FM non-alcoholic beverage with measurable amounts of health-promoting compounds. An ideal fermentation period (24 h) for FM malt non-alcoholic beverage production with enhanced health-promoting compounds, using Lactobacillus fermentum was demonstrated. Fermentation limit (96 h) for production of FM malt beverage using either the grain microbial flora or L. fermentum was confirmed. These findings provide a rationale for increased utilisation of FM as a functional food grain, and its use as malt in production of non-alcoholic beverage for health promotion and wellness. / NRF

DEd (Educational Management) / The purpose of this study was to investigate the leadership role of principals in creating a culture of teaching and learning in rural public schools of Mopani District, Limpopo Province. The performance of learners below the expected standards in the National Senior Certificate (NSC) examinations, despite, massive resources that the Department of Basic Education (DBE) is providing annually to schools to improve quality of teaching and learning, has raised public concern. When seeking answers to these schools’ dysfunctionality, focus, however, was laid on the leadership role of school principals in some rural public schools of Mopani District, Limpopo Province. The study was guided by Hersey and Blanchard Situational Leadership theory, complemented by Maslow’s Hierarchy of needs. This study was conceptualised within the interpretive paradigm, subsumed under a qualitative research approach. A qualitative case study was used with four rural public high schools. A total of sixteen participants were purposefully selected, made up of four school principals, four deputy principals and eight Heads of Departments (HoDs). Research data were gathered through individual interviews, observations and documents analysis. Data from interviews were thematically analysed, and data from documents and observations were analysed descriptively in words. Findings from interviews revealed that school principals do not monitor and support curriculum implementation. In supporting the findings from interviews, the documents analysed revealed the absence of an administered class-visits monitoring-tool. Data from the observations revealed that schools were without vision and mission statements and had inadequate resources. The study recommended principals to monitor and support curriculum implementation, and develop staff professionally. Suggestions for further study were provided. A proposed model of Action-Based COTL was developed to assist school principals in creating a COTL in schools. / NRF

MENVSC / Department of Ecology and Resource Management / Over the years it has been observed that the solid waste sector has been an increasingly major contributor to the amount of Greenhouse gases (GHGs) in the atmosphere. To some extent a great chunk of these GHGs in the atmosphere is from Landfill gas (LFG). This study employs two theoretical models (LandGEM and Afvalzorg models) to estimate the amount of LFG emitted from Weltervenden and Thohoyandou landfill sites located in Limpopo province of South Africa. Furthermore, the study investigated the appropriate technique of the LFG utilisation as a source of electricity and the number of households using electricity. LFGcost model was used to estimate the cost and benefits related to the implementation of a LFG utilisation technology. Also, the possible health and environmental impacts of the landfill emissions on the people living closer to the landfill site were determined. The LandGEM model’s simulation concludes that CH4 and CO2 peaked in the year 2020 with values of 3.323 × 103 Mg/year and 9.118 × 103 Mg/year, respectively, for the Thohoyandou landfill. Results from the Afvalzorg model indicate that the CH4 peaked in the year 2020 with value of 3.501 × 103 Mg/year. Meanwhile the total emission of CH4 from 2005-2040 by the LandGEM and Afvalzorg models are 66200 Mg/year and 69768 Mg/year, respectively. However, for the Weltervenden landfill, the total LFG peaked in the year 2023 while the CH4 peak at 4061 Mg/year and 3128 Mg/year for LandGEM and Afvalzorg models, respectively. Furthermore, results from the cost analysis and benefits for the implementation of a LFG utilisation technology in both landfills show that the implementation of such a utilisation technology will be economically feasible considering the sale of t CO2 equivalent in the carbon market. However, without considering the sales of t CO2 equivalent, not all the LFG engines are economically feasible for both landfills. This study also shows that the residents living closer to the Thohoyandou landfill are at a higher risk of environmental pollution and could suffer negative impacts from the landfill than residents living far from the landfill site. However, the Weltervenden landfill did not have lots of communities living closer to the landfill and therefore it was not included in this study. / NRF

MSc (Chemistry / Department of Chemistry / In this study, three sampling sites (Tshipise thermal spring, Mphephu thermal spring and Siloam borehole) in Limpopo Province South Africa were considered for an investigation into the mineral composition of their water and soil samples. Tshipise and Mphephu thermal springs are well developed and located within tourist resorts. On the other hand, Siloam is a borehole on the grounds of a private household. The water is used for various domestic purposes such as laundry, general cleaning of the household and bathing. Water and soil samples from the three sites were collected once per week every week in February 2018. The sampling bottles were spiked with 1M HNO3 before sampling to keep any metal ions present in the water samples in solution. The soil and water samples were analysed for their chemical composition using X-ray fluorescence (XRF) and inductively coupled plasma mass spectrometry (ICP-MS) respectively. XRF analysis of the soil samples found many major oxides of which SiO2 made up 63.67%, 85.37% and 46.28% by mass of Tshipise, Mphephu and Siloam soil samples respectively. XRF analysis also showed the presence of dangerous levels of heavy metals such as As, Tl, Pb and V. The analysis of the water samples by ICP-MS found that Tshipise, Mphephu and Siloam mineral water were soft, soft-moderately hard and soft-hard respectively with regards to the water hardness scale according to the United States Geological Survey (USGS) and the Water Quality Association (WQA). Analysis of water samples also found the presence of As, Cd, Cr and V. Arsenic was found to occur at concentrations above the Maximum Allowable Concentration (MAC) set by the World Health Organisation (WHO) (10 μg L-1) for Tshipise, Mphephu and Siloam mineral water (13.63 μg L-1, 15.83 μg L-1 and 20.97 μg L-1 respectively). Water temperatures for the three sites ranged from 38°C (Siloam borehole) to 64°C (Tshipise thermal spring). The pH values were 7.25 (Siloam borehole), 7.40 (Mphephu thermal spring) and 8.67 (Tshipise thermal spring). Due to the presence of As, Tl, Pb, V, Cd and Cr, the mineral water from all three thermal springs is unsafe to consume as it would lead to serious negative health effects some of which are outlined in this study. / NRF

PhD (Psychology) / Department of Psychology / The aim of the study was to develop a conceptualized model that will enhance language acquisition of children between 0 and 3 years. Language is a use of words and symbol to convey message, or communication. Generally, from birth to the age of four months children express their needs through crying and are soothed by the sound of voices or by low rhythmic sounds. When adults talk to children, children later imitate adult’s tongue movements and start to make sounds, coos and gurgles. Providing children with developmental stimulation and exposing them to learning opportunities increases their cognitive and social-emotional skills/abilities. Strategies to enhance language development are fundamental in children and can reduce many literacy related problems in later childhood. The objectives of the study are: to describe activities done by caregivers to influence language development; to identify factors that influence language development; to describe the importance of language development and to explain the disorders that are associated with language development. This study was qualitative in nature and it utilised an explorative design. This research design enabled the researcher to investigate the nature of acquisition comprehensively as well as the development of language and other factors that are related to it. The study was conducted at Vhembe District Municipality. Three villages were selected, namely Tshikuwi, Tshirolwe and HaMatsa villages, which are found in Makhado Municipality under Limpopo Province, South Africa. Purposive sampling was used to sample participants for the study. The individuals selected were those judged to have certain special characteristics as well as the ability to provide in-depth information for the purpose of the study. Thirty participants were selected to participate in the study. The semi-structured face to face interview and the interview guide with open-ended questions were used for data collection. Pre-testing of the interview guide was done to check if the guide would produce the expected results, three women from three different villages were selected for pretesting. All questions in the interview guide were addressed during data collection. Collected data were transcribed and analysed using thematic content analysis. The study adhered to the following research ethics: informed consent, anonymity, confidentiality, voluntary participation, no harm to the participants and dissemination of results. The study revealed that language is an important tool which is used to convey information from one person to the other. The findings indicate that language helps children to communicate and relate with other people. Language was also explained as a tool which is used to educate children, either at school or at home. The study shows that when children are learning language, they will also be stimulating their reasoning, thinking and problem-solving skills. In addition, it was also indicated that cognitive development reduces problems such as poor language development, a low self-esteem and isolation. The findings of this study show that there are a number of activities that can be used by caregivers and parents to foster the development of language in children. Those activities include, communicating with a child, reading of books, watching TV, naming of objects and explaining meaning of words. The study also identified several environmental factors that can be used to foster the development of language in children. The following environmental factors were identified in the study: communication, parental level of education, home socioeconomic status, caregiver’s personality and the availability of other children in the family. Lastly, the findings show that there are many disorders that may affect the development of language in children. The language disorders that the study identified include expressive language disorder, dyslexia, and inability to understand spoken. However, the study also reveals that these disorders may be treated. The findings of this study lead to the development of a conceptualized model to facilitate language acquisition in children between 0 and 3 years was developed. The meaning of one concept, namely reinforcement was explained in detail, in order for users of the model to understand its operational definition in the model. Reinforcement was explained because it has a diversity of meanings. The model enabled the researcher to create a link between research and what is happening in a society. Model validation was done to verify if the developed model relates to practical life, research and language development. The model for this study was validated for its applicability and usability. The drafted model was given to psychologists, speech therapists and parent to determine its usability and applicability. Keywords: language, language acquisition, language development model, language disorders. / NRF

MPH / Department of Public Health / Cerebral palsy (CP) is the common cause of physical disability in children. The nature and severity of impairments vary, but the demands placed upon mothers are significant. It is one of the prolonged conditions which have become a serious health problem worldwide. The purpose of this study was to explore and describe the challenges of mothers caring for cerebral palsy children at Thulamela Municipality, in the Vhembe District, South Africa. The objectives of this study were as follows: (1). To explore the challenges by mothers of cerebral palsy children in relation to physical, psychological and social care, (2) To explore their challenges in relation to available resources for proper referral of mothers caring for cerebral palsy children. The study used a qualitative approach using explorative and descriptive design in exploring the challenges of mothers caring for children with cerebral palsy. The study population comprised mothers of children with Cerebral Palsy living in the Vhembe District who attends the rehabilitation therapy services at Tshilidzini Hospital. A non-probability, purposive sampling technique was used to select the mothers. Five focus groups, each consisting of eight mothers were composed. Semi-structured group guide consisting of open-ended questions were used. Data was transcribed, verbatim and coded into themes and subthemes using context thematic analysis method. Themes that emerged include: a theoretical framework for the study, effects of caring on the family’s socio-economic life, challenges experienced by mothers caring for cerebral palsy children, challenges in relation to education and training and challenges experienced by mothers caring for cerebral palsy. The findings of the study might benefit the society about the challenges experienced by mothers when caring for their disabled children. The study might help government to develop and implement policies which will empower parents and the community as a whole / NRF

MCur / Department of Advanced Nursing Science / The Department of Education has stated that parents and guardians may not be absolved from their responsibilities regarding their pregnant child, and they should take the lead in working with the school to support and monitor their child’s health and progress. The purpose of this study was to explore and describe the role of parents regarding teenage pregnancy and school attendance in the rural village of Thomo in Mopani District Limpopo Province. The study is qualitative, explorative, descriptive and contextual in nature. The population comprised of the selected parents and caregivers residing at Thomo village of Mopani District. Purposive sampling was used to select 10 parents and 5 caregivers. Sampling size was determined by data saturation. Data was collected through in-depth individual interviews, guided by unstructured questions, data analysis was through open coding. Trustworthiness was ensured through credibility, dependability, conformability and transferability and ethical issues were adhered to. One main theme with four sub - themes emerged from the raw data; namely, parents /caregivers skills and personal factors that affect behaviour change; parents / caregivers intention to change the teenagers’ behaviour; environmental and cultural factors as facilitators/ barriers to pregnancy prevention and parent-school partnerships. Recommendations were made that included the following: awareness campaigns regarding prevention of teenage pregnancy; establishment of the compulsory education subject; review of existing teenage pregnancy policies; engaging parents on youth programs; using churches to strengthen youth education on the dangers of sex before marriage; as well as capacitating parents on effective communication with their children. / NRF

MENVSC / Department of Ecology and Resource Management / Mine tailings (MT) have been a global problem due to the environmental impacts the waste generates such as air, soil and water pollution. The detrimental impacts include a global problem such as acid mine drainage (AMD) which has been difficult to cleanup. Several studies have been conducted to find alternative measures in reducing or mitigating impacts such as AMD and air pollution. Several studies have revealed how alumino-silicate mineral waste can be used as raw material to produce construction materials. This study aimed at evaluating the potential of synthesizing a geopolymer material from Musina copper mine tailings. Tailings were characterized for their physicochemical and mineralogical compositions using standard laboratory techniques in order to evaluate suitability in geopolymerization. First section of the results presented physicochemical and mineralogical characterization of the Musina copper tailings together with the bioavailability of the chemical species. It was observed that the tailings are mainly composed of SiO2 and Al2O3 as the major oxides indicating that they are aluminosilicate material. Mineralogical analysis revealed dominance of quartz, epidote and chlorite as the major minerals. The bioavailability assessment showed that largely Cu and Ca are bioavailable and highly soluble in an aqueous solution while Al, Mg, Ni, Co, Cr and Fe have a high proportion in non-labile phase. Second section presented the preliminary results wherein the potential application of Musina copper tailings in geopolymerization was evaluated. The results showed that Musina copper tailings can be used to synthesize a geopolymer material. However, it was recommended that several parameters influencing geopolymerization need to be evaluated. The third section presented the evaluation of optimum parameters that influence the geopolymerization process, which include type of alkali activators, alkali activator concentration, curing temperature, liquid-solid (L/S) ratio and curing regime. It was observed that a mixture of NaOH:Na2SiO3.5H20 at a ratio of 70:30 yields a better geopolymer material. The concentration of 10 M NaOH:Na2SiO3.5H20 at a ratio of 70:30 was observed to be the best that yielded the UCS that is acceptable according to SANS1215 standards. When evaluating curing regime, it was found that the material cured using greenhouse has lower UCS as compared to the material cured using oven. The v effect of temperature showed that the UCS decreases with increasing curing temperature. An admixture of river sand and cement was introduced which resulted in a high UCS of 21.16 MPa when using an admixture of cement. The mineralogical composition of the geopolymer bricks showed formation of secondary minerals such as phlogopite, fluorapatite, diopside and actinolite. Batch leaching conducted on the geopolymer bricks detected high leaching of Na from the bricks. Based on the findings of the study of the raw MT potential to produce geopolymer bricks, it was concluded that the material can be used to produce bricks that are within the SANS 1215 requirements. The study further recommended that the study a focus on using cylindrical moulds, other alkali activators and a mechanical mixer. It was also recommended that the greenhouse be restructured to contain heat within the greenhouse during the evening so as to allow constant temperature within / NRF

MSc (Botany) / Department of Botany / Invasive alien plants (IAPs) are non-native species that have spread beyond the introduction site; become abundant to an extent that they serve as agents of change that also threaten native biological diversity (Garcia-Berthou, 2007). IAPs have medicinal applications for the treatment of various aliments in humans (Jäger et al., 1996). In this study, an ethnomedicinal survey was conducted on medicinal uses of invasive alien plant species in order to identify and investigate the various disease treatment methods employed by Bapedi traditional health practitioners within Capricorn District. Six traditional health practitioners were randomly selected, via the snowball method from each of the five local municipalities namely: Aganang, Blouberg, Lepelle-Nkupi, Molomole and Polokwane. The whole survey included 30 traditional health practitioners. A semi structured questionnaire was administered to document information about the medicinal uses of IAPs from participants. The collected data from the questionnaire was captured in Microsoft Excel 2010 and analyzed using descriptive statistics. The study recorded 22 IAPs belonging to 14 plant families. Records or data collected show invasive alien plants species to have been used for treatment of different alignments amongst Bapedi traditional health practitioners. Plant families with the most recorded species include: Solanaceae and Myrtaceae with 18.2% for both of them. The plant parts most frequently used in descending order were as follows: roots vii (40%), followed by the leaves (39%), fruits (7%), thorns (6%), bark (4%), seeds (2%) and lastly stems (2%). Decoction was recorded as the most dominant method used during medicinal preparations (n = 176) and flu was recorded as the most treated ailment amongst traditional health practitioners (n = 29). Apart from clearing IAPs as part of management strategy by South African government, there are other alternative innovative ways that can be employed to ensure that the number of IAPs introduced does not exceed beyond measure.This could be achieved by encouraging local people to use them as either medicinal sources or by incorporating them into any other local ethnobotanical use. The new innovative ways (such as traditional health practitioners utilizing the IAPs instead of endangered plants or indigenous plants species) can bring in some form of relief to the already declining indigenous species populations. Research scholars from different research institutes and higher education research centres can regard IAPs used medicinally as source of new pharmaceutical leads to create new medications. With such attention turned to alien invasive species, their survival as well as their spread will be kept on check at the same time creating a control measure to their spread with little effort. Further laboratory-based studies are needed to determine efficiency and safety of the IAPs used by traditional health practitioners in this study. / NRF

Thesis (M.Dev.) --University of Limpopo, 2007 / The study sought to develop guidelines that are aimed at improving Termination of Pregnancy (TOP) services that are rendered by public hospital based midwives. The researcher applied a qualitative and descriptive design. The study targeted midwives in the Bohlabela District, which has three hospitals. A non-probability purposive sampling was used to 6 midwives who are currently conducting TOP services in the hospitals. Data collection was both in-depth and conducted in face-to-face interviews with each participant. The findings of the study were analyzed, categorized into sub-themes, and revealed that midwives who conduct TOP services experience the following challenges, namely: inadequate human resource, poor infrastructure, lack of equipments, poor management support, and lack of support from doctors. Based on the findings of the study, it is imperative that all hospitals be designated centres for TOP services, so as to reduce the workload in the few hospitals that currently are inundated. The infrastructure needs to be improved, information about TOP services to the public has to be disseminated through awareness campaigns, and scarce skills allowances must be introduced. In addition, more staff members need to be employed. The study further recommends that the TOP policy guideline be reviewed in terms of allowing other competent health professionals to perform TOP. Chapter two discusses the literature review regarding the challenges that affect midwives who conduct TOP. In this study, theresearch compares the practice of TOP by the developing and developed countries, looks at related to laws at on TOP; gives an overview of sterilization Act in South Africa; mentions the amendments of laws on TOP; considers other legal restrictions; presents mandatory counseling for TOP clients; focuses on religious, cultural and traditional beliefs. The researcher has consulted different literatures, journals articles and website on challenges that affect midwives in rendering TOP in Limpopo, South Africa and world wild. Chapter three presents research methodology that consists of research design; area of study; population; sampling method; data collection method and procedure; ensuring trust worthiness; and ethical consideration, to be followed by limitation of the study. Chapter deals with data analysis and interpretation. Chapter five presents the researcher’s conclusions and recommendations.