We implement a package for the construction of a new class of Low Density Parity Check (LDPC) codes based on a new random high girth graph construction technique, and study the performance of the codes so constructed on both the Additive White Gaussian Noise (AWGN) channel as well as the Binary Erasure Channel (BEC). Our codes are “near regular”, meaning thereby that the the left degree of any node in the Tanner graph constructed varies by at most 1 from the average left degree and so also the right degree. The simulations for rate half codes indicate that the codes perform better than both the regular Progressive Edge Growth (PEG) codes which are constructed using a similar random technique, as well as the MacKay random codes. For high rates the ARG (Almost Regular high Girth) codes perform better than the PEG codes at low to medium SNR’s but the PEG codes seem to do better at high SNR’s. We have tried to track both near codewords as well as small weight codewords for these codes to examine the performance at high rates. For the binary erasure channel the performance of the ARG codes is better than that of the PEG codes. We have also proposed a modification of the sum-product decoding algorithm, where a quantity called the “node credibility” is used to appropriately process messages to check nodes. This technique substantially reduces the error rates at signal to noise ratios of 2.5dB and beyond for the codes experimented on. The average number of iterations to achieve this improved performance is practically the same as that for the traditional sum-product algorithm.

Le lait écrémé est utilisé depuis plus d’un demi-siècle comme diluant protecteur des spermatozoïdes de mammifères. Depuis quelques années, il existe une demande grandissante pour des diluants exempts de produits d’origine animale. Toutefois, le mécanisme par lequel le lait protège les spermatozoïdes n’est pas connu, ce qui rend difficile de lui trouver un substitut. Les protéines majeures du plasma séminal de taureau, les protéines « Binder of SPerm » (BSP), sont néfastes lors de la conservation de la semence. Les spermatozoïdes sont en contact avec une grande concentration de protéines BSP qui stimulent une extraction continuelle de cholestérol/phospholipides de leur membrane plasmique. Les lipoprotéines de faible densité (LDL) du jaune d’oeuf, un autre composé utilisé dans les diluants, empêcheraient les protéines BSP de se lier à la membrane des spermatozoïdes de taureaux et de stimuler un efflux des lipides membranaires, ce qui les protégerait durant la conservation. Notre hypothèse était que les protéines du lait protègent les spermatozoïdes durant la conservation en séquestrant les protéines BSP. Premièrement, nous avons démontré par filtration sur gel qu’il y a une interaction entre les protéines BSP bovines et les protéines du lait. Le lait écrémé a été fractionné en trois fractions : F1 (alpha-lactalbumine, bêta-lactoglobuline et caséine kappa), F2 (toutes les protéines du lait) et F3 (sels, sucres et petits peptides). Les protéines BSP1 et BSP5 ont une affinité plus grande pour F1 que BSP3, tandis que toutes les protéines BSP ont une affinité pour F2. Le titrage calorimétrique isotherme a permis de confirmer l’interaction entre les protéines BSP et les protéines du lait. L’association entre la protéine BSP1 bovine et les micelles de caséines est caractérisée par une constante d’affinité (Ka) de 3.5 × 10^5 M-1 et un paramètre stoichiométrique (n) de 4,5 BSP1 pour une caséine. L’association entre la protéine BSP1 bovine et l’alpha-lactalbumine (une protéine du sérum principale), est caractérisée par un Ka de 2.4 × 10^5 M-1 et une valeur “n” de 0,8. Ces résultats indiquent que le lait protège les spermatozoïdes bovins en séquestrant les protéines BSP grâce à une interaction protéine : protéine, tandis que le jaune d’oeuf les protège grâce à une interaction protéine : lipoprotéine. Deuxièmement, nous avons démontré par filtration sur gel que les protéines homologues aux BSP bovines retrouvées dans le plasma séminal de porc, d’étalon et de bélier ont une affinité avec les protéines du lait, ce qui suggère que le mécanisme de protection des spermatozoïdes par le lait pourrait être le même chez ces espèces. Troisièmement, nous avons caractérisé l’interaction entre BSP1 bovine et les LDL du jaune d’oeuf qui a un Ka de 3.4 ± 0.4 × 10^6 M-1 et une valeur de « n » de 104 BSP1 pour une particule de LDL, indiquant qu’il existe des différences entre le mécanisme de protection des spermatozoïdes par le lait et le jaune d’oeuf. Nous croyons que les résultats présentés dans cette thèse aideront à créer de nouveaux diluants ne contenant pas de produits d’origine animale afin de cryoconserver les spermatozoïdes des mammifères. / Skim milk is being used as a protective agent for mammalian semen conservation over half a century. Recently, there has been increased interest in developing extenders free of animal products. However, it is difficult to find suitable component in order to replace milk as an extender, because the mechanisms by which milk protect sperm against cooling and freezing damages during the storage is unknown. The Binder of SPerm (BSP) proteins are the major proteins of bull seminal plasma and they are harmful during sperm storage. In fact, sperm would be in contact with a large quantity of BSP proteins that induce a continuous cholesterol and phospholipids efflux from the sperm membrane during storage. When bull sperm is diluted with an extender containing egg yolk, another compound frequently used in extender, the low-density lipoproteins (LDL) present in the egg yolk prevent the binding of the BSP proteins to the sperm membrane, thus, preventing the lipid efflux from the sperm membrane induced by the BSP proteins. Our hypothesis was that milk proteins would protect sperm during storage by binding BSP proteins. First, we demonstrated by gel filtration that bovine BSP proteins could bind the milk proteins. Skim milk was fractionated into three fractions: F1 (alpha-lactalbumin and beta- lactoglobulin, the major whey proteins and kappa-casein), F2 (mainly caseins and all other milk proteins in small amounts) and F3 (salts, sugars and small peptides). Bovine BSP1 and BSP5 have more affinity for F1 as compared to BSP3 and all the BSP proteins have affinity for F2. We confirmed the interaction between bovine BSP proteins and milk proteins by isothermal titration calorimetry. The binding of BSP1 to casein micelles is characterized by an affinity constant (Ka) of 3.5 × 10^5 M-1 and of a stoichiometric parameter for the association (n) of 4.5 BSP1 per casein. The association between BSP1 and alpha-lactalbumin (one of the major whey proteins) is characterized by a Ka of 2.4 × 10^5 M-1 and a “n” value of 0.8. These results support our contention that milk can protect sperm by preventing the BSP proteins’ binding to the sperm membrane attributable to a protein : protein interaction, while egg yolk sperm protection is attributable to a protein : lipoprotein interaction. Second, our studies showed that the homologous BSP proteins found in the boar, stallion and ram seminal plasma can bind the milk proteins. These results indicate that the mechanism of sperm protection by milk in these species should be similar to the one in bovine species. Third, we characterized the interaction between bovine BSP1 protein and LDL from hen’s egg yolk. The binding was characterized by a Ka of 3.4 ± 0.4 × 10^6 M-1 and a « n » value of 104 BSP1 per LDL particle. Our results indicated that there is difference between the mechanism of sperm protection by milk and egg yolk. We believe that the results presented in this thesis may help to create new extenders free of animal product for mammal sperm preservation in liquid or frozen state.

CONTEXTE: L'inefficacité de captation des acides gras libres (AGL) par le tissu adipeux blanc (TAB) est connue pour favoriser la résistance à l'insuline (RI) dans les tissus périphériques, mais dans le foie, elle favorise également la production accrue de lipoprotéines contenant l'apolipoprotéine B100 (lipoprotéines apoB). Nous avons récemment démontré que les femmes post-ménopausées obèses avec un nombre élevé de lipoprotéines apoB à jeun (apoB plasmatique > 1,2 g/L) avaient plus de RI que les femmes avec un taux d’apoB normal. Notre objectif était donc d'examiner si l'inefficacité de captation des AGL pourrait être un mécanisme expliquant la RI, in vivo dans cette population. HYPOTHÈSES: Les femmes ménopausées en surpoids/obèses avec un taux d’apoB élevé ont moins d'efficacité à capter les AGL par le TAB que les femmes avec un taux d’apoB faible. MÉTHODES/RÉSULTATS: L'efficacité de captation des AGL a été examinée dans 22 femmes non diabétiques en surpoids/obèses. La population a été séparée selon la médiane d’apoB (0.9 g/L) en 2 groupes; les femmes ayant un taux d’apoB inférieur vs supérieur à la médiane (N=11/groupe). L'efficacité de captation des AGL par le TAB a été indirectement évaluée en suivant le sort d'un repas riche en gras (0.0162g 13C-trioléine/g de matières grasses, 66% de gras, 47g gras/m2 surface corporelle) marqué au 13C-trioléine, sur 6h en circulation ([13C]TG et [13C]AGL plasmatiques) et en oxydation (13CO2 dans l’air expiré [AE]). L’enrichissement en 13C des échantillons d’AE et du plasma a été mesuré par spectrométrie de masse pour ratio isotopique. Les femmes ayant un apoB élevé avaient une clairance plasmatique totale postprandiale des TG (p <0,05) réduite sans diminuer de la clairance plasmatique totale des AGL, par rapport aux femmes ayant un faible taux d’apoB. Cependant, en examinant le sort du 13C-trioléine, les femmes ayant un apoB élevé avait une réduction de la clairance des [13C]TG plasmatiques (44,78 μM vs 7,81 μM; p <0,05) et des [13C]AGL plasmatiques (2,64 uM vs 0,06 uM; p <0,05) à 6h, sans aucune différence en % récupéré de 13C-trioléine dans le CO2 de l’AE. Ces données suggèrent que les femmes ayant un taux d’apoB élevé ont une réduction postprandiale de la clairance et de la captation de 13Ctrioléine par le TAB. CONCLUSION: La captation inefficace des AGL par le TAB des femmes post-ménopausées en surpoids et obèses avec un surplus d’apoB peut être un mécanisme sousjacent à la RI chez ces sujets. / BACKGROUND: Inefficiency of fatty acid (FA) trapping in white adipose tissue (WAT) is known to promote insulin resistance (IR) in peripheral tissue, but in the liver, it also promotes increased secretion of apolipoprotein B100-lipoproteins (apoB-lipoproteins). We recently demonstrated that post-menopausal obese women with high numbers of fasting apoB-lipoproteins (plasma apoB> 1.2 g/L) had higher IR than women with normal apoB. Our aim was thus to examine whether inefficiency of FA trapping was a mechanism explaining IR in vivo in this population. HYPOTHESES: Postmenopausal overweight and obese women with high apoB have lower efficiency of FA trapping in WAT than women with low apoB. METHODS/RESULTS: The efficiency of FA trapping was examined in 22 non-diabetic overweight and obese women, and the group was separated around median apoB (0.9 g/L) into women with higher vs lower apoB (N=11 per group). The efficiency of FA trapping in WAT was indirectly assessed by following the fate of a 13C-triolein labelled high-fat meal (0.0162g 13C-triolein/g of fat, 66% fat, 47 g fat/m2 body surface area) over 6 hours in circulation (plasma [13C]TG and [13C]FA) and oxidation (breath 13CO2). 13Cenrichment of breath and plasma samples was measured by isotope ratio mass spectrometry. Women with higher apoB had delayed total postprandial plasma TG clearance (p<0.05) but not total plasma free FA clearance compared to women with lower apoB. However, examining the fate of 13C-triolein revealed that women with higher apoB had delayed plasma [13C]-TG clearance (44.78 μM vs 7.81 μM; p<0.05) and plasma [13C]-FA clearance (2.64 μM vs 0.06 μM; p<0.05) at 6 hours, without any differences in % recovered 13C-triolein in breath CO2. This data suggests that women with higher apoB have lower postprandial 13C-triolein clearance and trapping in WAT. CONCLUSION: Ineffective FA trapping in WAT in post-menopausal overweight and obese women with higher apoB may be a mechanism underlying IR in these subjects.

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal

This thesis investigates both theoretical and practical aspects of the design and analysis of modern error-control coding schemes, namely low-density parity-check (LDPC) codes and rateless codes for unequal error protection (UEP). It also studies the application of modern error-control codes in efficient data dissemination in wireless ad-hoc and sensor networks. Two methodologies for the design and analysis of UEP-LDPC codes are proposed. For these proposed ensembles, density evolution formulas over the binary erasure channel are derived and used to optimize the degree distribution of the codes. Furthermore, for the first time, rateless codes that can provide UEP are developed. In addition to providing UEP, the proposed codes can be used in applications for which unequal recovery time is desirable, i.e., when more important parts of data are required to be recovered faster than less important parts. Asymptotic behavior of the UEP-rateless codes under the iterative decoding is investigated. In addition, the performance of the proposed codes is examined under the maximum-likelihood decoding, when the codes have short to moderate lengths. Results show that UEP-rateless codes are able to provide very low error rates for more important bits with only a subtle loss in the performance of less important bits. Moreover, it is shown that given a target bit error rate, different parts of the information symbols can be decoded after receiving different numbers of encoded symbols. This implies that information can be recovered in a progressive manner, which is of interest in many practical applications such as media-on-demand systems. This work also explores fundamental research problems related to applying error-control coding such as rateless coding to the problem of reliable and energy-efficient broadcasting in multihop wireless ad-hoc sensor networks. The proposed research touches on the four very large fields of wireless networking, coding theory, graph theory, and percolation theory. Based on the level of information that each node has about the network topology, several reliable and energy-efficient schemes are proposed, all of which are distributed and have low complexity of implementation. The first protocol does not require any information about the network topology. Another protocol, which is more energy efficient, assumes each node has local information about the network topology. In addition, this work proposes a distributed scheme for finding low-cost broadcast trees in wireless networks. This scheme takes into account various parameters such as distances between nodes and link losses. This protocol is then extended to find low-cost multicast trees. Several schemes are extensively simulated and are compared.

Humans have two plasminogen activators (PAs), tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA), which generate plasmin to breakdown fibrin and other barriers to cell migration. Both PAs are used as pharmaceuticals but their efficacies are limited by their rapid clearance from the circulation, predominantly by parenchymal cells of the liver. At the commencement of the work presented here, the hepatic receptors responsible for mediating the catabolism of the PAs were little understood. tPA degradation by hepatic cell lines was known to depend on the formation of binary complexes with the major PA inhibitor, plasminogen activator inhibitor type-1 (PAI-1). Initial studies presented here established that uPA was catabolised in a fashion similar to tPA by the hepatoma cell line, HepG2. Other laboratories around this time found that the major receptor mediating the binding and endocytosis of the PAs is Low Density Lipoprotein Receptor-related Protein (LRP1). LRP1 is a giant 600 kDa protein that binds a range of structurally and functionally diverse ligands including, activated α2 macroglobulin, apolipoproteins, β amyloid precursor protein, and a number of serpin-enzymes complexes, including PA??PAI-1 complexes. Further studies for the work presented here centred on this receptor. By using radiolabelled binding assays, ligand blots, and Western blots on cultured cells, the major findings are that: (1) basal LRP1 expression on HepG2 is low compared to a clone termed, HepG2a16, but appears to increase in long term culture; (2) a soluble form of LRP1, which retains ligand-binding capacity, is present in human circulation; (3) soluble LRP1 is also present in cerebral spinal fluid where its role in neurological disorders such as Alzheimer??s disease is a developing area of interest; and (4) the release of LRP1 is a mechanism conserved in evolution, possibly as distantly as molluscs. The discovery, identification, and characterisation of soluble LRP1 introduces this protein in the human circulation, and presents a possible further level of regulation for its associated receptor system.

The objective of my work was to develop HDPE clay nanocomposites for packaging with superior barrier (gas and water) properties by economical processing technique. This work also represents a comparative study of thermoplastic nanocomposites for packaging based on linear low density polyethylene (LLDPE), high density polyethylene (HDPE) and Nylon12. In this study properties and processing of a series of linear low density polyethylene (LLDPE), high density polyethylene (HDPE) and Nylon 12 nanocomposites based on Na-MMT clay and two different aspect ratio grades of kaolinite clay are discussed.

No description available.

Multi-antenna wireless communication systems that employ a large number of antennas have recently stirred a lot of research interest. This is mainly due to the possibility of achieving very high spectral efficiency, power efficiency, and link reliability in such large-scale multiple-input multiple-output (MIMO) systems. An emerging architecture for large-scale multiuser MIMO communications is one where each base station (BS) is equipped with a large number of antennas (tens to hundreds of antennas) and the user terminals are equipped with fewer antennas (one to four antennas) each. The backhaul communication between base stations is also carried out using large number of antennas. Because of the high dimensionality of large-scale MIMO signals, the computational complexity of various transceiver operations can be prohibitively large. Therefore, low complexity techniques that scale well for transceiver signal processing in such large-scale MIMO systems are crucial. The transceiver operations of interest include signal encoding at the transmitter, and channel estimation, detection and decoding at the receiver. This thesis focuses on the design and analysis of novel low-complexity transceiver signal processing schemes for large-scale MIMO systems. In this thesis, we consider two types of large-scale MIMO systems, namely, massive MIMO systems and generalized spatial modulation MIMO (GSM-MIMO) systems. In massive MIMO, the mapping of information bits to modulation symbols is done using conventional modulation alphabets (e.g., QAM, PSK). In GSM-MIMO, few of the avail-able transmit antennas are activated in a given channel use, and information bits are conveyed through the indices of these active antennas, in addition to the bits conveyed through conventional modulation symbols. We also propose a novel modulation scheme named as precoder index modulation, where information bits are conveyed through the index of the chosen precoder matrix as well as the modulation symbols transmitted. Massive MIMO: In this part of the thesis, we propose a novel MIMO receiver which exploits channel hardening that occurs in large-scale MIMO channels. Channel hardening refers to the phenomenon where the off-diagonal terms of HH H become much weaker compared to the diagonal terms as the size of the channel gain matrix H increases. We exploit this phenomenon to devise a low-complexity channel estimation scheme and a message passing algorithm for signal detection at the BS receiver in massive MIMO systems. We refer to the proposed receiver as the channel hardening-exploiting message passing (CHEMP) receiver. The key novelties in the proposed CHEMP receiver are: (i) operation on the matched filtered system model, (ii) Gaussian approximation on the off-diagonal terms of the HH H matrix, and (iii) direct estimation of HH H instead of H and use of this estimate of HH H for detection The performance and complexity results show that the proposed CHEMP receiver achieves near-optimal performance in large-scale MIMO systems at complexities less than those of linear receivers like minimum mean squared error (MMSE) receiver. We also present a log-likelihood ratio (LLR) analysis that provides an analytical reasoning for this better performance of the CHEMP receiver. Further, the proposed message passing based detection algorithm enables us to combine it with low density parity check (LDPC) decoder to formulate a joint message passing based detector-decoder. For this joint detector-decoder, we design optimized irregular binary LDPC codes specific to the massive MIMO channel and the proposed receiver through EXIT chart matching. The LDPC codes thus obtained are shown to achieve improved coded bit error rate (BER) performance compared to off-the-shelf irregular LDPC codes. The performance of the CHEMP receiver degrades when the system loading factor (ratio of the number of users to the number of BS antennas) and the modulation alpha-bet size are large. It is of interest to devise receiver algorithms that work well for high system loading factors and modulation alphabet sizes. For this purpose, we propose a low-complexity factor-graph based vector message passing algorithm for signal detection. This algorithm uses a scalar Gaussian approximation of interference on the basic sys-tem model. The performance results show that this algorithm performs well for large modulation alphabets and high loading factors. We combine this detection algorithm with a non-binary LDPC decoder to obtain a joint detector-decoder, where the field size of the non-binary LDPC code is same as the size of the modulation alphabet. For this joint message passing based detector-decoder, we design optimized non-binary irregular LDPC codes tailored to the massive MIMO channel and the proposed detector. GSM-MIMO: In this part of the thesis, we consider GSM-MIMO systems in point-to-point as well as multiuser communication settings. GSM-MIMO has the advantage of requiring only fewer transmit radio frequency (RF) chains than the number of transmit antennas. We analyze the capacity of point-to-point GSM-MIMO, and obtain lower and upper bounds on the GSM-MIMO system capacity. We also derive an upper bound on the BER performance of maximum likelihood detection in GSM-MIMO systems. This bound is shown to be tight at moderate to high signal-to-noise ratios. When the number of transmit and receive antennas are large, the complexity of en-coding and decoding of GSM-MIMO signals can be prohibitively high. To alleviate this problem, we propose a low complexity GSM-MIMO encoding technique that utilizes com-binatorial number system for bits-to-symbol mapping. We also propose a novel layered message passing (LaMP) algorithm for decoding GSM-MIMO signals. Low computational complexity is achieved in the LaMP algorithm by detecting the modulation bits and the antenna index bits in two deferent layers. We then consider large-scale multiuser GSM-MIMO systems, where multiple users employ GSM at their transmitters to communicate with a BS having a large number of receive antennas. For this system, we develop computationally efficient message passing algorithms for signal detection using vector Gaussian approximation of interference. The performance results of these algorithms show that the GSM-MIMO system outperforms the massive MIMO system by several dBs for the same spectral efficiency. Precoder index modulation: It is known that the performance of a communication link can be enhanced by exploiting time diversity without reducing the rate of transmission using pseudo random phase preceding (PRPP). In order to further improve the performance of GSM-MIMO, we apply PRPP technique to GSM-MIMO systems. PRPP provides additional diversity advantage at the receiver and further improves the performance of GSM-MIMO systems. For PRPP-GSM systems, we propose methods to simultaneously precode both the antenna index bits and the modulation symbols using rectangular precoder matrices. Finally, we extend the idea of index modulation to pre-coding and propose a new modulation scheme referred to as precoder index modulation (PIM). In PIM, information bits are conveyed through the index of a prehared PRPP matrix, in addition to the information bits conveyed through the modulation symbols. PIM is shown to increase the achieved spectral efficiency, in addition to providing diver-sity advantages.

The theoretical part of the thesis is devoted to the issue thermal insulation plasters which can be applied in the remediation of buildings. The practical part deals with optimizing the composition of thermal insulation plaster-based lightweight aggregate of the foam glass and with possible substitution of cement for other binders with latent hydraulic properties. The resultant plaster should meet the best ratio of mechanical and thermal insulating properties.