The modern psychiatric crisis has become a global epidemic as the prevalence of psychiatric disorders continues to rise. These numbers have only been exacerbated by the COVID-19 pandemic, and the long-term psychological effects resulting from the social isolation and fear of the disease have yet to be seen. Those already suffering from psychiatric disorders have limited options for treatment, as current therapeutic methods for mood, anxiety, and substance use disorders demonstrate high failure rates with many individuals suffering from treatment resistance. Individuals that do respond to modern therapies typically require treatment for several weeks or months, occasionally even years, before experiencing any reductions in their symptoms. Tryptamine psychedelics have been used for millennia by indigenous cultures through highly ritualized religious ceremonies to purge individuals of disease and offer spiritual guidance, however their application within modern medicine did not begin until Albert Hofmann’s discovery of LSD in 1938. The next several decades saw an explosion in clinical studies involving tryptamine psychedelics showcasing their ability to demonstrate immediate treatment after single doses in individuals suffering from diseases such as depression and PTSD, however research and clinical practices came to a halt after the passing of the Controlled Substances Act of 1970. As renewed interest in psychedelic medicine continues to grow, this review details the therapeutic potential of tryptamine psychedelics while exploring their current status within clinical trials.

Lysosomal storage diseases (LSDs) are metabolic diseases which occur as a result of a deficiency of one of the essential lysosomal enzymes, called glycohydrolases. A mutation in the gene encoding one of these enzymes leads to an accumulation of unwanted substrates, resulting in a variety of clinical manifestations. A common symptom found in LSDs is skeletal muscle dysfunction, which includes muscle weakness, atrophy and loss of muscle mass. The genes for lysosomal hydrolases are well characterized; however, much less is known about how mutations in these genes affect the cell and lead to the muscle dysfunction observed. One pathway of interest is autophagy; it has been shown to be essential for maintenance of skeletal muscles. This study sought to investigate the impact of LSDs on autophagy and how this may potentiate muscle dysfunction. We utilized in-vivo and in-vitro models of Sialidosis, Sandhoff Disease, and GM1-Gangliosidosis in order to assess autophagy and its impact on myogenic differentiation in skeletal muscles. Our results demonstrated that autophagy is induced upstream (ULK1 phosphorylation) but is inhibited at the autophagosome to lysosome fusion (p62 upregulation) in LSDs. We also found that myoblast fusion and myogenic differentiation are impaired. We conclude that blocking autophagy impairs myogenic differentiation, which potentiates the muscle dysfunction observed in LSDs. This work highlights autophagy as a new pathway of interest and possible therapeutic target to alleviate muscle dysfunction in LSDs, and other similar neurodegenerative diseases. / Thesis / Master of Science (MSc) / Lysosomal storage diseases (LSDs) occur because of a deficiency of lysosomal glycohydrolases. A common symptom found in LSDs is skeletal muscle dysfunction. Little is known about how a deficiency of these enzymes leads to the clinical manifestations observed. However, one pathway of interest is autophagy. This study sought to investigate the impact of LSDs on autophagy and how this may potentiate muscle dysfunction. We utilized in-vivo and in-vitro models of LSDs to assess autophagy and its impact on myogenic differentiation in skeletal muscles. We demonstrated that autophagy is induced and blocked, and that myoblast fusion and myogenic differentiation is impaired. We concluded that the induction and block of autophagy impairs myogenic differentiation, which potentiates muscle dysfunction.

This thesis is missing page 129, no other copy of the thesis has this page. Based on the figure list and last page, it is our belief that the thesis was incorrectly number and should end on Figure 17. -Digitization Centre / Many medical researchers in the post-WWII era explored LSD for its potential therapeutic value. Among these psychiatrists Humphry Osmond (in Weyburn) and Abram Hoffer (in Saskatoon) directed some of the most comprehensive trials in the Western world. These Saskatchewan-based medical researchers were first drawn to LSD because of its ability to produce a "model psychosis." Their experiments with the drug that Osmond was to famously describe as a "psychedelic"-led them to hypothesise, and promote, the biochemical constitution of Schizophrenia. Simulating psychotic symptoms through auto-experimentation, professionals also believed that the drug would help reform mental health accommodations by cultivating a sophisticated appreciation for the relationship between environment and health. This thesis examines the era of pre-criminal LSD experimentation. Drawing on hospital records, interviews with former research subjects, and the private papers of Hoffer and Osmond this dissertation will demonstrate that these LSD trials, far from fringe medical research, represented a fruitful and indeed encouraging branch of psychiatric research. Clinical LSD experiments in the 1950s played an influential role in defining theoretical and practical aims of the post-war psychiatric profession. Ultimately the experiments failed for two reasons, one scientific and the other cultural. The scientific parameters of clinical trials in medicine shifted in the 1950s and early 1960s so as to necessitate controlled trials (which the Saskatchewan researchers had failed to construct). Second, as LSD became increasingly associated with student riots, anti-war demonstrations and the counter culture, governments intervened to criminalise the drug, in effect terminating formal medical research with LSD. An historical examination of these LSD experiments provides insight into the changing complexion of psychiatry in the post-World War Two period, and the ways in which scientific medicine was shaped by social, cultural and political currents. / Dissertation / Doctor of Philosophy (PhD)

Após um intervalo de duas décadas, ressurgiu um novo interesse em estudos sobre alucinógenos que visam a compreensão de como estes compostos interagem com o sistema nervoso central (SNC). Sabendo-se que enzimas do tipo peroxidases estão presentes em células do tipo leucócitos, neurônios e microglia, e que, são capazes de oxidar compostos indólicos, esta, portanto, poderia representar uma rota ativa de metabolização de alucinógenos no SNC, ainda não conhecida. Nesta perspectiva, este trabalho contribui com a descrição da metabolização da dietilamida do ácido lisérgico (LSD) e da N,N-dimetiltriptamina (DMT) por peroxidase de rábano (HRP) e mieloperoxidase (MPO) proveniente de neutrófilos ativados. A formação de produtos de reação foi acompanhada por HPLC com detectores de arranjo de diodos (DAD) e fluorescência, e a identificação por espectrometria de massas (MS). Ambas as peroxidases foram capazes de metabolizar LSD a compostos que coincidem com produtos de sua metabolização in vivo, como 2-oxo-3-hidroxi-LSD (O-H-LSD) e nor-LSD, por enzimas hepáticas do complexo P450. Entretanto, um terceiro produto formado não havia sido descrito anteriormente. Apresenta como característica principal a abertura do anel indólico e foi nomeado pelo nosso grupo como N,N-dietil-7-formamido-4-metil-6-oxo-2,3,4,4a,5,6-hexahidrobenzo[f]quinolina-2-carboxamida (FOMBK). De uma maneira semelhante, HRP e MPO também metabolizaram DMT a um produto hidroxilado (OH-DMT), que possivelmente apresenta considerável ação alucinógena, e a um segundo produto nomeado N,N-dimetil-N-formil-quinuramina (DMFK). Visto que peroxidases estão presentes em diferentes tipos celulares, é razoável supor que a formação dos produtos descritos neste estudo possa ocorrer in vivo, numa possível via alternativa de metabolização de LSD e DMT ainda não descrita em humanos. / After a gap of two decades a new interest in hallucinogen studies that aim the comprehension of how these compounds interact with the central nervous system (CNS) rose again. It is known that peroxidases enzymes are present in cells such as leukocytes, neurons and microglia and that they are capable of oxidizing indolic compounds. Then it could represent an active metabolization pathway for hallucinogens in the CNS, not known yet. In this perspective, this study contributed with the description of the metabolization of lysergic acid diethylamide (LSD) and N,N-dimethyltryptamine (DMT) by horseradish peroxidase (HRP) and myeloperoxidase (MPO) from activated neutrophils. The formation of the reaction products was attended by HPLC with diode array and fluorescence detectors, and the identification by mass spectrometry (MS). Both peroxidases were capable of metabolizing LSD to compounds that coincide with products from its in vivo metabolization, as 2-oxo-3-hydroxy-LSD (O-H-LSD) and nor-LSD by the liver enzymes from P450 complex. However, a third compound had not been described before. It has the opened indolic ring as main characteristic and was named by our group as N,N-diethyl-7-formamido-4-methyl-6-oxo-2,3,4,4a,5,6-hexahydrobenzo[f]quinoline-2-carboxamide (FOMBK). In a similar way, HRP and MPO also metabolized DMT to a hydroxylated product (OH-DMT) that possibly shows a considerable hallucinogen action and to a second product named as N,N-dimethyl-N-formyl-kynuramine (DMFK). Since peroxidases are present in different cell types, it is reasonable to assume that the formation of the products described in this study may occur in vivo as well, in a possible alternative metabolic pathway for LSD and DMT that has not been described in humans yet.

Investigação sobre a noção de \"alquimia do teatro\" a partir da leitura do livro autobiográfico Mare Nostrum - sonhos, viagens e outros caminhos, de Fauzi Arap, realizada como estudo para a criação de uma dramaturgia baseada no depoimento. À partir da narrativa, que contempla o envolvimento do artista numa psicoterapia experimental baseada no uso psiquiátrico do LSD, identifica-se um paralelo entre a trajetória do autor e a dinâmica do mito associado à arte teatral - o mito grego Dioniso - e se reflete sobre as circunstâncias em que se deu a criação de sua obra, com foco no período da contracultura histórica brasileira. Buscando delinear a noção sugerida no capítulo 20 do livro \"o teatro da alquimia e a alquimia do teatro\", discute-se a questão do \"teatro ritual\" e se resgata a qualidade alquímica do mito de Dioniso, esboçando-se a noção de uma \"alquimia dionisíaca\". / Research on the notion of \"theater alchemy\" from reading the autobiographical book Mare Nostrum - dreams, travels and other ways, by Fauzi Arap, carried out as a study for the creation of a dramaturgy based on the testimony. From the narrative, which contemplates the artist\'s involvement in experimental psychotherapy based on the psychiatric use of LSD, a parallel is identified between the trajectory of the author and the dynamics of the myth associated with theatrical art - the Greek myth Dioniso - and is reflected on the circumstances in which the creation of his theatrical work took place, focusing on the period of Brazilian historical counterculture. Seeking to outline the notion suggested in Chapter 20 of the book \"The Theater of Alchemy and the Alchemy of Theater,\" the question of \"ritual theater\" is discussed and the alchemical quality of the Dionysian myth is rescued, outlining the notion of a \"Dionysian alchemy\".

Investigação sobre a noção de \"alquimia do teatro\" a partir da leitura do livro autobiográfico Mare Nostrum - sonhos, viagens e outros caminhos, de Fauzi Arap, realizada como estudo para a criação de uma dramaturgia baseada no depoimento. À partir da narrativa, que contempla o envolvimento do artista numa psicoterapia experimental baseada no uso psiquiátrico do LSD, identifica-se um paralelo entre a trajetória do autor e a dinâmica do mito associado à arte teatral - o mito grego Dioniso - e se reflete sobre as circunstâncias em que se deu a criação de sua obra, com foco no período da contracultura histórica brasileira. Buscando delinear a noção sugerida no capítulo 20 do livro \"o teatro da alquimia e a alquimia do teatro\", discute-se a questão do \"teatro ritual\" e se resgata a qualidade alquímica do mito de Dioniso, esboçando-se a noção de uma \"alquimia dionisíaca\". / Research on the notion of \"theater alchemy\" from reading the autobiographical book Mare Nostrum - dreams, travels and other ways, by Fauzi Arap, carried out as a study for the creation of a dramaturgy based on the testimony. From the narrative, which contemplates the artist\'s involvement in experimental psychotherapy based on the psychiatric use of LSD, a parallel is identified between the trajectory of the author and the dynamics of the myth associated with theatrical art - the Greek myth Dioniso - and is reflected on the circumstances in which the creation of his theatrical work took place, focusing on the period of Brazilian historical counterculture. Seeking to outline the notion suggested in Chapter 20 of the book \"The Theater of Alchemy and the Alchemy of Theater,\" the question of \"ritual theater\" is discussed and the alchemical quality of the Dionysian myth is rescued, outlining the notion of a \"Dionysian alchemy\".

O LSD, abreviação para a dietilamida do ácido lisérgico, é um alucinógeno que comumente é consumido na forma de selos e suas apreensões são constantes no cenário policial brasileiro e mundial. No momento da apreensão, nos laboratórios forenses tem-se um processo de análises químicas para a confirmação desta droga. As metodologias analíticas precisam ser sensíveis, específicas e fornecer resultados confiáveis, o que o processo de validação através de protocolos normatizados - pode garantir. Neste trabalho, propõem-se procedimentos experimentais para a determinação de LSD em amostras apreendidas através de técnicas de Voltametria Cíclica e Cromatografia Líquida de Alta Eficiência (CLAE) com detecções de arranjo de diodo e eletroquímica. Essas análises permitem ampliar as restritas metodologias encontradas na literatura. Esses procedimentos tiveram o perclorato de amônio em metanol com presença de água como eletrólito de suporte para a detecção voltamétrica, e com base nesses resultados, obteve-se informações para a detecção eletroquímica dentro do sistema cromatográfico. Esta mesma composição de fase móvel também permite a detecção por arranjo de diodo neste sistema. Nos três sistemas estudados, obteve os limites de quantificação de 1,64 10-6 mol L-1, 6,67 10-6 mol L-1 e 3,29 10-6 mol L-1 para as técnivas de Voltametria Cíclica, CLAE com detecção eletroquímica e detecção de arranjo de diodo, respectivamente. Tais valores mostram que as metodologias tem capacidade de analisar baixas concentrações de LSD. Além da validação dos três métodos propostos, fez-se a análise de três amostras apreendidas, das quais uma continha LSD e outras duas não. Deste modo, a partir da combinação dos mesmos componentes de fase móvel, é possível obter dois procedimentos cromatográficos baseados em diferentes tipos de detecção, enquanto que a determinação voltamétrica habilita outro tipo de análise aliando a portabilidade e rapidez da quantificação. Ao fim de todo o processo de validação envolvido, obtêm-se três metodologias que permitem a quantificação de LSD, com a confiabilidade garantida para serem aplicadas em análises de rotina de um laboratório forense. / LSD abbreviation for Lysergic Acid Diethylamide - is a hallucinogenic drug commonly consumed in blotters form. Inside the police scenario, its apprehension is constant in national and global scale. Once the drug is apprehended, forensic laboratories must follow a procedure with chemical analysis aiming a confirmatory result. The analytical methodologies applied for this purposes must be sensitive, specific and provide reliable results, and all these characteristics can be afforded with a validation process behind these procedures. In the present study, three new methodologies for the determination of LSD in seized samples are presented, based on Cyclic Voltammetry technique and High Performance Liquid Chromatography (HPLC) with diode array detection and electrochemical detection. These analyses increase the number of methodologies found in literature for the same purpose. All the experimental procedures included ammonium perchlorate in methanol with the presence of water composing the supporting electrolyte solution for voltammetric measurements. From the electrochemical information observed, it could be applied for electrochemical detection in a chromatographic system. This same composition as mobile phase enables a signal for diode array detection. In all the three systems, the values for limit of quantification were 1.64 10-6 mol L-1, 6.67 10-6 mol L-1 and 3.29 10-6 mol L-1, for voltammetric determination, HPLC with electrochemical detector and diode array detector, respectively. These results prove the methodologies can quantify low concentration of LSD in seized samples. Indeed, after all validation process, three real blotter samples were analyzed, which one of them contained LSD. In this way, from the same components in mobile phase, it is possible to gather two different procedures based on distinct detection principle, whilst the voltammetric determination might be seen as a variety for drug analysis, with portability and quickness as some characteristics for its quantification. Finally, after all validation process applied, there are three methodologies for quantification of LSD in seized samples, with confidence ensured for appliance in the routine of a forensic laboratory.

O LSD, abreviação para a dietilamida do ácido lisérgico, é um alucinógeno que comumente é consumido na forma de selos e suas apreensões são constantes no cenário policial brasileiro e mundial. No momento da apreensão, nos laboratórios forenses tem-se um processo de análises químicas para a confirmação desta droga. As metodologias analíticas precisam ser sensíveis, específicas e fornecer resultados confiáveis, o que o processo de validação através de protocolos normatizados - pode garantir. Neste trabalho, propõem-se procedimentos experimentais para a determinação de LSD em amostras apreendidas através de técnicas de Voltametria Cíclica e Cromatografia Líquida de Alta Eficiência (CLAE) com detecções de arranjo de diodo e eletroquímica. Essas análises permitem ampliar as restritas metodologias encontradas na literatura. Esses procedimentos tiveram o perclorato de amônio em metanol com presença de água como eletrólito de suporte para a detecção voltamétrica, e com base nesses resultados, obteve-se informações para a detecção eletroquímica dentro do sistema cromatográfico. Esta mesma composição de fase móvel também permite a detecção por arranjo de diodo neste sistema. Nos três sistemas estudados, obteve os limites de quantificação de 1,64 10-6 mol L-1, 6,67 10-6 mol L-1 e 3,29 10-6 mol L-1 para as técnivas de Voltametria Cíclica, CLAE com detecção eletroquímica e detecção de arranjo de diodo, respectivamente. Tais valores mostram que as metodologias tem capacidade de analisar baixas concentrações de LSD. Além da validação dos três métodos propostos, fez-se a análise de três amostras apreendidas, das quais uma continha LSD e outras duas não. Deste modo, a partir da combinação dos mesmos componentes de fase móvel, é possível obter dois procedimentos cromatográficos baseados em diferentes tipos de detecção, enquanto que a determinação voltamétrica habilita outro tipo de análise aliando a portabilidade e rapidez da quantificação. Ao fim de todo o processo de validação envolvido, obtêm-se três metodologias que permitem a quantificação de LSD, com a confiabilidade garantida para serem aplicadas em análises de rotina de um laboratório forense. / LSD abbreviation for Lysergic Acid Diethylamide - is a hallucinogenic drug commonly consumed in blotters form. Inside the police scenario, its apprehension is constant in national and global scale. Once the drug is apprehended, forensic laboratories must follow a procedure with chemical analysis aiming a confirmatory result. The analytical methodologies applied for this purposes must be sensitive, specific and provide reliable results, and all these characteristics can be afforded with a validation process behind these procedures. In the present study, three new methodologies for the determination of LSD in seized samples are presented, based on Cyclic Voltammetry technique and High Performance Liquid Chromatography (HPLC) with diode array detection and electrochemical detection. These analyses increase the number of methodologies found in literature for the same purpose. All the experimental procedures included ammonium perchlorate in methanol with the presence of water composing the supporting electrolyte solution for voltammetric measurements. From the electrochemical information observed, it could be applied for electrochemical detection in a chromatographic system. This same composition as mobile phase enables a signal for diode array detection. In all the three systems, the values for limit of quantification were 1.64 10-6 mol L-1, 6.67 10-6 mol L-1 and 3.29 10-6 mol L-1, for voltammetric determination, HPLC with electrochemical detector and diode array detector, respectively. These results prove the methodologies can quantify low concentration of LSD in seized samples. Indeed, after all validation process, three real blotter samples were analyzed, which one of them contained LSD. In this way, from the same components in mobile phase, it is possible to gather two different procedures based on distinct detection principle, whilst the voltammetric determination might be seen as a variety for drug analysis, with portability and quickness as some characteristics for its quantification. Finally, after all validation process applied, there are three methodologies for quantification of LSD in seized samples, with confidence ensured for appliance in the routine of a forensic laboratory.

Após um intervalo de duas décadas, ressurgiu um novo interesse em estudos sobre alucinógenos que visam a compreensão de como estes compostos interagem com o sistema nervoso central (SNC). Sabendo-se que enzimas do tipo peroxidases estão presentes em células do tipo leucócitos, neurônios e microglia, e que, são capazes de oxidar compostos indólicos, esta, portanto, poderia representar uma rota ativa de metabolização de alucinógenos no SNC, ainda não conhecida. Nesta perspectiva, este trabalho contribui com a descrição da metabolização da dietilamida do ácido lisérgico (LSD) e da N,N-dimetiltriptamina (DMT) por peroxidase de rábano (HRP) e mieloperoxidase (MPO) proveniente de neutrófilos ativados. A formação de produtos de reação foi acompanhada por HPLC com detectores de arranjo de diodos (DAD) e fluorescência, e a identificação por espectrometria de massas (MS). Ambas as peroxidases foram capazes de metabolizar LSD a compostos que coincidem com produtos de sua metabolização in vivo, como 2-oxo-3-hidroxi-LSD (O-H-LSD) e nor-LSD, por enzimas hepáticas do complexo P450. Entretanto, um terceiro produto formado não havia sido descrito anteriormente. Apresenta como característica principal a abertura do anel indólico e foi nomeado pelo nosso grupo como N,N-dietil-7-formamido-4-metil-6-oxo-2,3,4,4a,5,6-hexahidrobenzo[f]quinolina-2-carboxamida (FOMBK). De uma maneira semelhante, HRP e MPO também metabolizaram DMT a um produto hidroxilado (OH-DMT), que possivelmente apresenta considerável ação alucinógena, e a um segundo produto nomeado N,N-dimetil-N-formil-quinuramina (DMFK). Visto que peroxidases estão presentes em diferentes tipos celulares, é razoável supor que a formação dos produtos descritos neste estudo possa ocorrer in vivo, numa possível via alternativa de metabolização de LSD e DMT ainda não descrita em humanos. / After a gap of two decades a new interest in hallucinogen studies that aim the comprehension of how these compounds interact with the central nervous system (CNS) rose again. It is known that peroxidases enzymes are present in cells such as leukocytes, neurons and microglia and that they are capable of oxidizing indolic compounds. Then it could represent an active metabolization pathway for hallucinogens in the CNS, not known yet. In this perspective, this study contributed with the description of the metabolization of lysergic acid diethylamide (LSD) and N,N-dimethyltryptamine (DMT) by horseradish peroxidase (HRP) and myeloperoxidase (MPO) from activated neutrophils. The formation of the reaction products was attended by HPLC with diode array and fluorescence detectors, and the identification by mass spectrometry (MS). Both peroxidases were capable of metabolizing LSD to compounds that coincide with products from its in vivo metabolization, as 2-oxo-3-hydroxy-LSD (O-H-LSD) and nor-LSD by the liver enzymes from P450 complex. However, a third compound had not been described before. It has the opened indolic ring as main characteristic and was named by our group as N,N-diethyl-7-formamido-4-methyl-6-oxo-2,3,4,4a,5,6-hexahydrobenzo[f]quinoline-2-carboxamide (FOMBK). In a similar way, HRP and MPO also metabolized DMT to a hydroxylated product (OH-DMT) that possibly shows a considerable hallucinogen action and to a second product named as N,N-dimethyl-N-formyl-kynuramine (DMFK). Since peroxidases are present in different cell types, it is reasonable to assume that the formation of the products described in this study may occur in vivo as well, in a possible alternative metabolic pathway for LSD and DMT that has not been described in humans yet.

Nos últimos anos, vários métodos eletroanalíticos foram desenvolvidos a fim de tornarem mais práticas e acessíveis às análises químicas de substâncias entorpecentes realizadas pela polícia científica. Com a sensibilidade e seletividade compatíveis com as dos métodos instrumentais convencionais, é a portabilidade da técnica eletroanalítica que se sobressai e tem apresentado grande potencial para tais aplicações. O uso de complexos metálicos com as bases de Schiff como modificadores do eletrodo de trabalho, tornou ainda mais promissora a utilização destes métodos para a detecção e quantificação de entorpecentes. Os eletrodos de pasta de carbono também contribuem para a praticidade da eletroanalítica, uma vez que são produzidos de forma simples e barata. Desta forma, neste projeto foram desenvolvidos métodos voltamétricos para a quantificação da dietilamida do ácido lisérgico (LSD), utilizando-se o eletrodo de pasta de carbono modificado com o complexo [UO2(Ac-ophen)]·H2O. A aplicação do KCl como eletrólito suporte, em solução aquosa, diferencia estes métodos do encontrado na literatura. Nas análises, foram empregadas as técnicas de voltametria cíclica, onda quadrada e de pulso diferencial. A linearidade no aumento da corrente frente às variações da concentração do LSD possibilitou a obtenção das curvas analíticas com desvio padrão e limites de detecção e quantificação de 2,45, 0,62 e 1,02 mol L-1, respectivamente, como melhores resultados. As análises de recuperação das amostras, 103 e 108%, demostra a possibilidade da utilização destas no âmbito forense. / The development of electroanalytical methods for forensic science has been growing in recent years by the fact of their practicality and low cost. Once sensibility and selectivity values are compatible to conventional methods, as chromatography and spectrometry, the portability of this system consists on interesting advantages. The simple modification of the carbon paste working electrode with Schiff bases complexes became even more promising to use this method for the detection and quantification of narcotics. The carbon paste electrode itself contributes for the practicality of the analyses, once it is made of simple carbon powder, which can be disposable. This project aimed to develop voltammetric methods for the quantification of the lysergic acid diethylamide (LSD) using a carbon paste electrode modified with the complex [UO2(Ac-ophen)]·H2O. The use of aqueous solution of KCl as supporting electrolyte characterizes a less pollutant methodology, differently of other methods that still use toxic solvents The combination of the differential pulse and square wave voltammetries with the modified carbon paste was crucial for the detection of trace levels of the LSD. The linear response in various concentrations of LSD results on analytical curves with standard deviation, detection, and quantification limits of 2,45, 0,62, 1,02 mol L-1, respectively. And the recovery values of 103 and 108 % indicates the possibility of using this method in the forensic science