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Inseminação artificial pós-cervical em tempo fixo em porcas recebendo pLH no início do estro / Fixed-time post cervical artificial insemination in sows receiving pLH at estrus onsetFontana, Diogo Luiz January 2013 (has links)
Inseminação artificial em tempo fixo (IATF) associada à inseminação artificial pós-cervical (IAPC) permite uma maior utilização de machos geneticamente superiores e uma redução expressiva de mão de obra na produção de suínos. O objetivo deste estudo foi avaliar a eficiência da IATF de acordo com diferentes protocolos de (IA), usando pLH - hormônio luteinizante suíno - como indutor da ovulação. Um total de 597 matrizes desmamadas com detecção de estro realizada uma vez ao dia (08:00) foram alocados em três tratamentos: Controle (n = 199) - primeira inseminação realizada no início do estro (0 h) e repetida a cada 24 h, durante o estro; IATF1 (n = 199) - fêmeas receberam 5 mg (4 ml) i.m. de pLH no início do estro, e foram inseminadas 24 horas depois, e IATF2 (n = 199) – fêmeas receberam 5 mg de pLH mas foram inseminadas no início do estro (0 h) e 24 horas depois. Foram realizadas IAPC com doses homospérmicas (1,5 x 109 de espermatozoides totais/50 ml) em todos os tratamentos. O tratamento hormonal não afetou o intervalo do início do estro à ovulação (P> 0,05). O número de inseminações foi de 2,9, 1,0 e 2,0 para Controle, FTAI1 e FTAI2 respectivamente. Não houve diferença entre os tratamentos para taxa de parto e leitões nascidos totais (P> 0,05). Leitões nascidos totais por dose inseminante foi diferente (P <0,0001) entre os tratamentos (4,5, 12,5 e 6,2 para Controle, FTAI1 e FTAI2 respectivamente). O uso de pLH no início do estro associado à uma única inseminação em tempo fixo IAPC 24 horas após, não comprometeu o desempenho reprodutivo de porcas multíparas. / Fixed-time artificial insemination (FTAI) associated to post cervical artificial insemination (PCAI) allows a wider use of high indexed boars and an expressive reduction on labor requirements in swine production. The aim of this study was to evaluate FTAI efficiency according to different AI protocols, using pLH – porcine luteinizing hormone - as ovulation inductor. A total of 597 weaned sows whose estrus detection was performed once daily (08:00 am) were allocated into three treatments: Control (n= 199) – the first insemination was performed at estrus onset (0 h) and repeated every 24 h thereafter, during estrus; FTAI1 (n= 199) - sows received a 5 mg (4 ml) i.m. injection of pLH at estrus onset, and were inseminated 24 h after, and FTAI2 (n= 199) - sows received 5 mg of pLH but were inseminated at estrus onset (0 h) and 24 h after. PCAI with homospermic doses (1.5 x 109 total sperm cells/50 ml) were performed in all treatments. Hormonal treatment did not affect the interval onset of estrus to ovulation (P>0.05). The number of inseminations was 2.9, 1.0 and 2.0 for Control, FTAI1 and FTAI2 respectively. Treatments did not affect farrowing rate and total born (P>0.05). Total piglets born per insemination dose was different (P<0.0001) among treatments (4.5, 12.5 and 6.2 for Control, FTAI1 and FTAI2 respectively). The use of pLH at estrus onset associated to a single fixed-time PCAI 24 h after does not compromise the reproductive performance of multiparous sows.
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Expressão gênica do receptor do hormônio luteinizante (LHR), em células da teca e da granulosa de folículos antrais bovinosNogueira, Marcelo Fábio Gouveia [UNESP] January 2005 (has links) (PDF)
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nogueira_mfg_dr_botfmvz.pdf: 892396 bytes, checksum: 471e1cec0bdbf86073d92bc94c87f460 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Em células da teca e da granulosa, de folículos bovinos, foram detectados quatro transcritos alternativos do receptor do hormônio luteinizante (LHR). Apenas dois deles são traduzidos em proteínas funcionais com afinidades distintas em relação aos ligantes. Em humanos e símios, a isoforma completa (full-length) tem afinidade pelo LH e hCG, enquanto que a isoforma que apresenta deleção do exon 10 tem afinidade somente pelo hCG. Além disso, isoformas com deleção do exon 3 foram observadas em ratos, embora nenhum outro estudo tenha investigado essa região do gene bovino. Objetivouse com este trabalho caracterizar o padrão da expressão do gene do LHR nas células da teca e da granulosa de folículos antrais bovinos. Ovários foram coletados em matadouro, os folículos (5-14 mm) foram dissecados e as células da teca e da granulosa separadas para extração de RNA total com Trizol. As concentrações de esteróides no fluido folicular foram determinadas por radioimunoensaio (RIE). A expressão gênica do LHR foi mensurada por RTPCR semiquantitativo com oligonucleotídeos iniciadores (primers) específicos para amplificar o fragmento entre o final da região extracelular e o final da intracelular (LHRBC; primers posicionados nos exons 9 e 11). A ocorrência de transcritos alternativos oriundos do início da região extracelular (LHRA; primers posicionados nos exons 2 e 9) foi investigada mediante amplificação por RT-PCR. Como controle interno no PCR, utilizou-se a expressão da GAPDH. Paralelamente, células da granulosa cultivadas in vitro foram tratadas com 1 ou 10 ng de FSH no meio de cultura. Mediante RT-PCR, foi investigada a expressão das isoformas do LHRBC nas células da granulosa cultivadas e tratadas com FSH... / Growth of dominant follicle in the absence of circulating FSH and the events following the LH surge that culminate in ovulation, are dependent on the interaction between LH and its receptor (LHR). Four LHR alternative transcripts were described in theca and granulosa cells from bovine follicles. Only two of them can be translated to functional proteins (receptors coupled with G protein) with different affinities to their ligands. In humans and marmosets, the full-length isoform has affinity to both LH and hCG molecules, whereas the isoform with deletion of only exon 10 has affinity to hCG exclusively. Additionally, isoforms with deletion of exon 3 were observed in rats, although no previous report have investigated this region of the bovine gene. The objective of this study was to characterize the pattern of gene expression of the LHR in theca and granulosa cells from bovine antral follicles. Additionally, LHR expression was determined in cultured granulosa cells under FSH treatment. From ovaries collected in abattoir, antral follicles were dissected (5 to 14mm of diameter), and samples of theca and granulosa cells were obtained to total RNA extraction (Trizol protocol). Steroids concentrations in the follicular fluid were determined by RIA. Gene expression of LHR was measured by semiquantitative RT-PCR with specific primers to amplify part of extracellular region (LHRA; primers annealing on exons 2 and 9) and the fragment from the end of extracellular region, including the transmembrane domain and finishing near the end of intracellular region (LHRBC; primers annealing on exons 9 and 11). As internal control of the PCR, it was used GAPDH expression. Cultured granulosa cells were treated with 0, 1 or 10 ng of FSH (3 replicates each dose). As in vivo and positive control, theca cell sample was utilized to comparison... (Complete abstract, access undermentioned electronic address)
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Studies on Regulation of Rat Corpus Luteum Function by Prolactin And Luteinizing HormoneJohn, Miya January 2015 (has links) (PDF)
The corpus luteum (CL) is a transient endocrine structure formed from the remnants of an ovulated follicle with the primary purpose of producing progesterone (P4), a hormone vital for the establishment and maintenance of pregnancy. The precise regulation of CL function is essential for normal reproductive cycles and maintenance of early pregnancy. In mammals, the pituitary hormones prolactin (PRL) and luteinizing hormone (LH) function as luteotrophic factors during pregnancy, and these two hormones form a functional luteotrophic complex to control CL function in rodents. The mechanistic underpinnings of the luteotrophic actions of PRL and LH, as well as the interplay between the two hormones are poorly understood, and has been the focus of the current investigation. There are several limitations involved in studying luteal function under cell culture conditions. Hence, in vivo animal models employing dopaminergic receptor agonist, 2-Bromo-α-Ergocryptine Mesylate (CB-154; inhibits pituitary PRL secretion) and GnRH receptor antagonist, cetrorelix (CET; inhibits pituitary LH secretion) have been standardized for purposes of examining the roles of PRL and LH in the regulation of CL structure and function in rats. Administration of CB-154 or CET to pregnant rats caused inhibition of CL function and concomitant loss of conceptuses. The CB-154 treatment induced loss of implants was determined to be the result of inhibition of luteal function, rather than the non-specific effects of CB-154 or requirement of PRL for uterine maintenance of implants. To understand how PRL and LH regulate luteal function, targets of PRL and LH in the rat CL needs to be established; however, this has not been well defined by previous studies. The present study observed that CB-154 induced inhibition of luteal function was gradual in its onset; hence, transcriptional changes of genes involved in steroid genesis were examined. mRNA expression of genes involved in P4 production were found to be down regulated, while 20α-hydroxysteroid dehydrogenate (20α-HSD), a P4 catabolizing enzyme was unregulated by CB-154 treatment. CET treatment also had a similar effect on mRNA expression of steroidogenic genes. Interestingly, mRNA expression of the steroidogenic acute regulatory protein (StAR), a key regulator of steroid genesis was not regulated by CB-154 or CET treatment. The luteolytic factor PGF2α also inhibited CL function in pregnant rats but did not down regulate mRNA expression of StAR. However, examination of phospho-StAR (Ser-195), the activated form of StAR, during CET and PGF2α-induced luteolysis suggested that regulation of StAR in the CL of pregnant rats might primarily be at the level of phosphorylation. PRL has been implicated in maintaining luteal expression of LH/choriogonadotrophin receptor (LH/CGR), the cognate receptor for LH. Hence, the luteotrophic actions of PRL may be indirect, by way of regulating LH signalling. Hence, the importance of the LH/CGR pathway and its regulation were examined. LH/CGR mRNA expression was found to correlate with CL function, with CET and CB-154 treatments resulting in down regulation of LH/CGR mRNA expression. Further, CB-154 treatment down regulated LH/CGR pre-mRNA levels, suggesting a role for PRL in the regulation of LH/CGR transcription. mRNA expression of LRH-1, a constitutively active transcription factor previously reported to be important in CL function was down regulated by both CB-154 and CET treatments and hence correlated with LH/CGR mRNA expression. Further, luciferase assays in HeLA cells transiently expressing LRH-1 suggests its involvement in activating the LH/CGR promoter. Estrogen receptor (ER)-α and ER-β also appear to correlate with LH/CGR expression and may play a role along with LRH-1 in the regulation of LH/CGR mRNA expression in the CL of pregnant rats. To examine mechanisms by which PRL may regulate its downstream targets, pathways employed by PRL in the CL of pregnant rats were analysed. The Akt pathway including downstream targets were down regulated by CB-154 treatment. The pathway was found to be regulated at the level of Akt1 mRNA expression. Hence, actions of PRL may regulate the survival of CL. This study has also made observations of LH playing a similar role in survival of the CL. The results of these studies taken together, shed light on the regulation of CL structure and function by PRL and LH, and provide molecular evidence for the two hormones having similar downstream targets and functioning as a luteotrophic complex in pregnant rats, which could only mean a robust interaction between the signalling pathways employed by the two hormones.
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Expressão gênica do receptor do hormônio luteinizante (LHR), em células da teca e da granulosa de folículos antrais bovinos /Nogueira, Marcelo Fábio Gouveia. January 2005 (has links)
Orientador: Ciro Moraes Barros / Resumo: Em células da teca e da granulosa, de folículos bovinos, foram detectados quatro transcritos alternativos do receptor do hormônio luteinizante (LHR). Apenas dois deles são traduzidos em proteínas funcionais com afinidades distintas em relação aos ligantes. Em humanos e símios, a isoforma completa ("full-length") tem afinidade pelo LH e hCG, enquanto que a isoforma que apresenta deleção do exon 10 tem afinidade somente pelo hCG. Além disso, isoformas com deleção do exon 3 foram observadas em ratos, embora nenhum outro estudo tenha investigado essa região do gene bovino. Objetivouse com este trabalho caracterizar o padrão da expressão do gene do LHR nas células da teca e da granulosa de folículos antrais bovinos. Ovários foram coletados em matadouro, os folículos (5-14 mm) foram dissecados e as células da teca e da granulosa separadas para extração de RNA total com Trizol. As concentrações de esteróides no fluido folicular foram determinadas por radioimunoensaio (RIE). A expressão gênica do LHR foi mensurada por RTPCR semiquantitativo com oligonucleotídeos iniciadores ("primers") específicos para amplificar o fragmento entre o final da região extracelular e o final da intracelular (LHRBC; "primers" posicionados nos exons 9 e 11). A ocorrência de transcritos alternativos oriundos do início da região extracelular (LHRA; "primers" posicionados nos exons 2 e 9) foi investigada mediante amplificação por RT-PCR. Como controle interno no PCR, utilizou-se a expressão da GAPDH. Paralelamente, células da granulosa cultivadas in vitro foram tratadas com 1 ou 10 ng de FSH no meio de cultura. Mediante RT-PCR, foi investigada a expressão das isoformas do LHRBC nas células da granulosa cultivadas e tratadas com FSH... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Growth of dominant follicle in the absence of circulating FSH and the events following the LH surge that culminate in ovulation, are dependent on the interaction between LH and its receptor (LHR). Four LHR alternative transcripts were described in theca and granulosa cells from bovine follicles. Only two of them can be translated to functional proteins (receptors coupled with G protein) with different affinities to their ligands. In humans and marmosets, the full-length isoform has affinity to both LH and hCG molecules, whereas the isoform with deletion of only exon 10 has affinity to hCG exclusively. Additionally, isoforms with deletion of exon 3 were observed in rats, although no previous report have investigated this region of the bovine gene. The objective of this study was to characterize the pattern of gene expression of the LHR in theca and granulosa cells from bovine antral follicles. Additionally, LHR expression was determined in cultured granulosa cells under FSH treatment. From ovaries collected in abattoir, antral follicles were dissected (5 to 14mm of diameter), and samples of theca and granulosa cells were obtained to total RNA extraction (Trizol protocol). Steroids concentrations in the follicular fluid were determined by RIA. Gene expression of LHR was measured by semiquantitative RT-PCR with specific primers to amplify part of extracellular region (LHRA; primers annealing on exons 2 and 9) and the fragment from the end of extracellular region, including the transmembrane domain and finishing near the end of intracellular region (LHRBC; primers annealing on exons 9 and 11). As internal control of the PCR, it was used GAPDH expression. Cultured granulosa cells were treated with 0, 1 or 10 ng of FSH (3 replicates each dose). As in vivo and positive control, theca cell sample was utilized to comparison... (Complete abstract, access undermentioned electronic address) / Doutor
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Inseminação artificial pós-cervical em tempo fixo em porcas recebendo pLH no início do estro / Fixed-time post cervical artificial insemination in sows receiving pLH at estrus onsetFontana, Diogo Luiz January 2013 (has links)
Inseminação artificial em tempo fixo (IATF) associada à inseminação artificial pós-cervical (IAPC) permite uma maior utilização de machos geneticamente superiores e uma redução expressiva de mão de obra na produção de suínos. O objetivo deste estudo foi avaliar a eficiência da IATF de acordo com diferentes protocolos de (IA), usando pLH - hormônio luteinizante suíno - como indutor da ovulação. Um total de 597 matrizes desmamadas com detecção de estro realizada uma vez ao dia (08:00) foram alocados em três tratamentos: Controle (n = 199) - primeira inseminação realizada no início do estro (0 h) e repetida a cada 24 h, durante o estro; IATF1 (n = 199) - fêmeas receberam 5 mg (4 ml) i.m. de pLH no início do estro, e foram inseminadas 24 horas depois, e IATF2 (n = 199) – fêmeas receberam 5 mg de pLH mas foram inseminadas no início do estro (0 h) e 24 horas depois. Foram realizadas IAPC com doses homospérmicas (1,5 x 109 de espermatozoides totais/50 ml) em todos os tratamentos. O tratamento hormonal não afetou o intervalo do início do estro à ovulação (P> 0,05). O número de inseminações foi de 2,9, 1,0 e 2,0 para Controle, FTAI1 e FTAI2 respectivamente. Não houve diferença entre os tratamentos para taxa de parto e leitões nascidos totais (P> 0,05). Leitões nascidos totais por dose inseminante foi diferente (P <0,0001) entre os tratamentos (4,5, 12,5 e 6,2 para Controle, FTAI1 e FTAI2 respectivamente). O uso de pLH no início do estro associado à uma única inseminação em tempo fixo IAPC 24 horas após, não comprometeu o desempenho reprodutivo de porcas multíparas. / Fixed-time artificial insemination (FTAI) associated to post cervical artificial insemination (PCAI) allows a wider use of high indexed boars and an expressive reduction on labor requirements in swine production. The aim of this study was to evaluate FTAI efficiency according to different AI protocols, using pLH – porcine luteinizing hormone - as ovulation inductor. A total of 597 weaned sows whose estrus detection was performed once daily (08:00 am) were allocated into three treatments: Control (n= 199) – the first insemination was performed at estrus onset (0 h) and repeated every 24 h thereafter, during estrus; FTAI1 (n= 199) - sows received a 5 mg (4 ml) i.m. injection of pLH at estrus onset, and were inseminated 24 h after, and FTAI2 (n= 199) - sows received 5 mg of pLH but were inseminated at estrus onset (0 h) and 24 h after. PCAI with homospermic doses (1.5 x 109 total sperm cells/50 ml) were performed in all treatments. Hormonal treatment did not affect the interval onset of estrus to ovulation (P>0.05). The number of inseminations was 2.9, 1.0 and 2.0 for Control, FTAI1 and FTAI2 respectively. Treatments did not affect farrowing rate and total born (P>0.05). Total piglets born per insemination dose was different (P<0.0001) among treatments (4.5, 12.5 and 6.2 for Control, FTAI1 and FTAI2 respectively). The use of pLH at estrus onset associated to a single fixed-time PCAI 24 h after does not compromise the reproductive performance of multiparous sows.
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Identificação imunohistoquímica de receptores para hormônio luteinizante, estrôgeno e progesterona no trato reprodutivo extragonadal da égua / Immunohistochemical identification of luteinizing, estrogen and progesterone receptors in the extra-gonadal reproductive tract of maresEsmeraldino, Anamaria Telles January 2012 (has links)
O objetivo deste trabalho foi verificar a presença e a localização de receptores para hormônios esteróides e gonadotróficos, através da técnica de imunohistoquímica, pelo método de peroxidase-antiperoxidase (PAP), nos diferentes tecidos que compõe o trato genital da égua e a variação de reatividade destes receptores durante o ciclo estral e no anestro fisiológico. Também se objetivou verificar se há diferença de reatividade em éguas com e sem endometrose. Foram coletadas amostras de útero, cérvice e oviduto, de 41 éguas sem raça definida e com histórico reprodutivo desconhecido, em um abatedouro. Quinze éguas se encontravam em estro, dezoito em diestro e oito éguas em anestro. Concluiu-se que a intensidade e a distribuição da coloração para os receptores de estrógeno (RE), progesterona (RP) e hormônio luteinizante (RLH) variaram de acordo com o tipo de célula e o estágio do ciclo estral. Nas amostras de endométrio observou-se imunorreatividade alta no epitélio luminal para RE e RP tanto no estro quanto no diestro; o epitélio glandular, estroma e miométrio mostraram reatividade moderada para os dois receptores durante as duas fases. Durante o anestro os resultados foram semelhantes aos encontrados durante a fase cíclica. Na avaliação da reatividade para RLH, durante o estro e diestro, o epitélio luminal mostrou reatividade de fraca a moderada, mas no diestro houve maior reatividade média. O epitélio glandular apresentou menor reatividade do que o luminal. No miométrio a coloração foi fraca durante todo o ciclo. Durante o anestro a reatividade foi fraca no epitélio luminal, ausente em quase todas as amostras no epitélio glandular e de fraca a ausente no miométrio. Neste experimento, não foi observada diferença significativa de reatividade entre os endométrios com e sem endometrose, mas as áreas afetadas mostraram coloração assíncrona para RE, RP e RLH. Na cérvice, foi observada imunorreatividade moderada a alta para RE e RP no epitélio luminal, no estroma e no músculo. A intensidade de coloração das células epiteliais e musculares variou pouco entre o estro e o diestro, mas durante o anestro houve maior reatividade no tecido muscular e no estroma. Foi observada reatividade para RLH no epitélio e camada muscular, sem variação significativa nas fases do ciclo. A intensidade de coloração foi de fraca a moderada no epitélio e fraca na camada muscular. No oviduto, observou-se imunorreatividade para RE e RP nos três tecidos, durante a fase cíclica e o anestro. No epitélio, os valores encontrados foram de moderados a altos, sem variação significativa nas três fases. A coloração das células epiteliais do oviduto foi nitidamente irregular, com o núcleo muito corado no que parecem ser células secretoras e pouco corado ou sem coloração nas células ciliadas, refletindo provavelmente as diferentes funções das células epiteliais neste órgão. No estroma a reatividade foi moderada durante a fase luteal, mostrando reatividade mais alta no estro e no anestro. A camada muscular apresentou reatividade máxima para RE no estro e no diestro. A reatividade para RLH no epitélio luminal foi de fraca a moderada durante todo o ciclo. No músculo também foi observada reatividade, porém bem mais fraca do que no epitélio. Durante o anestro somente três das oito amostras apresentaram reatividade no tecido muscular. No diestro foi observada maior reatividade do que no estro. Os resultados do presente estudo evidenciam, pela primeira vez, a presença de receptores para LH nos diferentes tecidos do trato reprodutor extragonadal da égua. Embora existam relatos da expressão e localização de RE e RP no endométrio equino, esta é a primeira vez que se utiliza a técnica de imunohistoquímica para localizar estes receptores na cérvice e no oviduto desta espécie. Foi observada variação individual bastante acentuada entre as amostras, em uma mesma fase cíclica. Provavelmente estes resultados sejam o reflexo da variação entre o dia do ciclo em que os animais se encontravam, bem como da complexidade dos mecanismos envolvidos na presença desses receptores. Os achados deste estudo indicam que tanto os hormônios gonadais quanto o LH atuam por meio de seus receptores nos diferentes tecidos do trato reprodutivo da égua, podendo servir para a elaboração de novas estratégias para melhorar a eficiência reprodutiva nesta espécie. / The aim of this study was to demonstrate the presence and localization of gonadotropic and steroid hormone receptors, in different tissues of the mare genital tract and the different reactivity to these receptors during the endometrial cycle and physiologic anestrus. Another objective was to compare the reactivity to theses receptors in mares with and without endometrosis. Immunohistochemistry was performed using the peroxidase anti-peroxidase technique (PAP). Uterus, cervix and oviduct of 41 criollo mares were collected in an abattoir. There was variation in the intensity of the staining and distribution for estrogen receptors (PR), progesterone receptors (PR) and luteinizing hormone receptors (LHR) with the endometrial cycle and different tissues. The endometrial surface epithelial cells were stained strongly for ER and PR in the estrous and dioestrus; glandular epithelial cells, stromal cells and smooth muscle cells of the myometrium had moderate staining for ER and PR during these two phases and in anestrus too. The immunoreactive score for LHR in the surface epithelial cells during endometrial cycle was weak to moderate but, in general, strong staining was observed in dioestrus. More weak staining intensity was observed in the glandular epithelial cells than luminal epithelial cells. Smooth muscle cells of the myometrium showed weak staining for LHR throughout the endometrial cycle. During the anestrus, the immunoreactivity score was weak in the surface epithelial cells. In general, the glandular epithelium was not stained. Myometrium cells were weak to not staining for LHR, in this phase. In this study there was no significant difference in immunoreactive score for ER, PR and LHR in endometrium with or without endometrosis but fibrotic glands showed different expression patterns of ER, PR and LHR, could evidence for functionally glandular maldifferentiation in endometrosis. The cervical epithelial surface, stromal cells and smooth muscle cells were moderate to strongly staining for ER and PR, with little variation throughout the endometrial cycle but the immunorectivity was strongest during the anestrus in muscular and stromal cells. Surface epithelial cells of cervix were weak to moderate stained for LHR; smooth muscle cells showed weak staining for these receptors. There was no variation during cycle. In the oviduct, epithelial, stromal and muscle cells showed reactivity for RE and RP, during cycle and anestrus. Epithelial cells were moderate to strongly staining for these receptors, with evident irregularity in different types of cells. Apparently ciliated epithelial cells were stained but the intensity was much less than that observed in nonciliated epithelial cells, probably reflecting different functions of these cells. Stromal cells showed moderate staining during dioestrus and strongest reactivity in estrous and anestrus; muscle cells showed strong reactivity for ER throughout the cycle. The reactivity for LHR was weak to moderate throughout the cycle in the epithelial cells and weak in the muscle cells. During anestrus only three strains of muscle cells showed reactivity for LHR. In dioestrus the intensity was strongest. These findings evidence for the firs time the presence for LHR in extra-gonadal reproductive organs of mare. Though there were reports of ER and PR expression in equine endometrium, this is the first report of localization of these receptors in cervix and oviduct of mare using immunohistochemistry. It was found marked individual variation among the strains. These results probably were caused by the variation among the day of cycle and the complexity of mechanisms involved in the presence of these receptors. The findings of the present study allow us to infer that the ovarian steroid hormones nad LH function through their receptors in different tissues of mare reproductive tract, can help us to elaborate new strategies to improve the reproductive efficiency in this specie.
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Identificação imunohistoquímica de receptores para hormônio luteinizante, estrôgeno e progesterona no trato reprodutivo extragonadal da égua / Immunohistochemical identification of luteinizing, estrogen and progesterone receptors in the extra-gonadal reproductive tract of maresEsmeraldino, Anamaria Telles January 2012 (has links)
O objetivo deste trabalho foi verificar a presença e a localização de receptores para hormônios esteróides e gonadotróficos, através da técnica de imunohistoquímica, pelo método de peroxidase-antiperoxidase (PAP), nos diferentes tecidos que compõe o trato genital da égua e a variação de reatividade destes receptores durante o ciclo estral e no anestro fisiológico. Também se objetivou verificar se há diferença de reatividade em éguas com e sem endometrose. Foram coletadas amostras de útero, cérvice e oviduto, de 41 éguas sem raça definida e com histórico reprodutivo desconhecido, em um abatedouro. Quinze éguas se encontravam em estro, dezoito em diestro e oito éguas em anestro. Concluiu-se que a intensidade e a distribuição da coloração para os receptores de estrógeno (RE), progesterona (RP) e hormônio luteinizante (RLH) variaram de acordo com o tipo de célula e o estágio do ciclo estral. Nas amostras de endométrio observou-se imunorreatividade alta no epitélio luminal para RE e RP tanto no estro quanto no diestro; o epitélio glandular, estroma e miométrio mostraram reatividade moderada para os dois receptores durante as duas fases. Durante o anestro os resultados foram semelhantes aos encontrados durante a fase cíclica. Na avaliação da reatividade para RLH, durante o estro e diestro, o epitélio luminal mostrou reatividade de fraca a moderada, mas no diestro houve maior reatividade média. O epitélio glandular apresentou menor reatividade do que o luminal. No miométrio a coloração foi fraca durante todo o ciclo. Durante o anestro a reatividade foi fraca no epitélio luminal, ausente em quase todas as amostras no epitélio glandular e de fraca a ausente no miométrio. Neste experimento, não foi observada diferença significativa de reatividade entre os endométrios com e sem endometrose, mas as áreas afetadas mostraram coloração assíncrona para RE, RP e RLH. Na cérvice, foi observada imunorreatividade moderada a alta para RE e RP no epitélio luminal, no estroma e no músculo. A intensidade de coloração das células epiteliais e musculares variou pouco entre o estro e o diestro, mas durante o anestro houve maior reatividade no tecido muscular e no estroma. Foi observada reatividade para RLH no epitélio e camada muscular, sem variação significativa nas fases do ciclo. A intensidade de coloração foi de fraca a moderada no epitélio e fraca na camada muscular. No oviduto, observou-se imunorreatividade para RE e RP nos três tecidos, durante a fase cíclica e o anestro. No epitélio, os valores encontrados foram de moderados a altos, sem variação significativa nas três fases. A coloração das células epiteliais do oviduto foi nitidamente irregular, com o núcleo muito corado no que parecem ser células secretoras e pouco corado ou sem coloração nas células ciliadas, refletindo provavelmente as diferentes funções das células epiteliais neste órgão. No estroma a reatividade foi moderada durante a fase luteal, mostrando reatividade mais alta no estro e no anestro. A camada muscular apresentou reatividade máxima para RE no estro e no diestro. A reatividade para RLH no epitélio luminal foi de fraca a moderada durante todo o ciclo. No músculo também foi observada reatividade, porém bem mais fraca do que no epitélio. Durante o anestro somente três das oito amostras apresentaram reatividade no tecido muscular. No diestro foi observada maior reatividade do que no estro. Os resultados do presente estudo evidenciam, pela primeira vez, a presença de receptores para LH nos diferentes tecidos do trato reprodutor extragonadal da égua. Embora existam relatos da expressão e localização de RE e RP no endométrio equino, esta é a primeira vez que se utiliza a técnica de imunohistoquímica para localizar estes receptores na cérvice e no oviduto desta espécie. Foi observada variação individual bastante acentuada entre as amostras, em uma mesma fase cíclica. Provavelmente estes resultados sejam o reflexo da variação entre o dia do ciclo em que os animais se encontravam, bem como da complexidade dos mecanismos envolvidos na presença desses receptores. Os achados deste estudo indicam que tanto os hormônios gonadais quanto o LH atuam por meio de seus receptores nos diferentes tecidos do trato reprodutivo da égua, podendo servir para a elaboração de novas estratégias para melhorar a eficiência reprodutiva nesta espécie. / The aim of this study was to demonstrate the presence and localization of gonadotropic and steroid hormone receptors, in different tissues of the mare genital tract and the different reactivity to these receptors during the endometrial cycle and physiologic anestrus. Another objective was to compare the reactivity to theses receptors in mares with and without endometrosis. Immunohistochemistry was performed using the peroxidase anti-peroxidase technique (PAP). Uterus, cervix and oviduct of 41 criollo mares were collected in an abattoir. There was variation in the intensity of the staining and distribution for estrogen receptors (PR), progesterone receptors (PR) and luteinizing hormone receptors (LHR) with the endometrial cycle and different tissues. The endometrial surface epithelial cells were stained strongly for ER and PR in the estrous and dioestrus; glandular epithelial cells, stromal cells and smooth muscle cells of the myometrium had moderate staining for ER and PR during these two phases and in anestrus too. The immunoreactive score for LHR in the surface epithelial cells during endometrial cycle was weak to moderate but, in general, strong staining was observed in dioestrus. More weak staining intensity was observed in the glandular epithelial cells than luminal epithelial cells. Smooth muscle cells of the myometrium showed weak staining for LHR throughout the endometrial cycle. During the anestrus, the immunoreactivity score was weak in the surface epithelial cells. In general, the glandular epithelium was not stained. Myometrium cells were weak to not staining for LHR, in this phase. In this study there was no significant difference in immunoreactive score for ER, PR and LHR in endometrium with or without endometrosis but fibrotic glands showed different expression patterns of ER, PR and LHR, could evidence for functionally glandular maldifferentiation in endometrosis. The cervical epithelial surface, stromal cells and smooth muscle cells were moderate to strongly staining for ER and PR, with little variation throughout the endometrial cycle but the immunorectivity was strongest during the anestrus in muscular and stromal cells. Surface epithelial cells of cervix were weak to moderate stained for LHR; smooth muscle cells showed weak staining for these receptors. There was no variation during cycle. In the oviduct, epithelial, stromal and muscle cells showed reactivity for RE and RP, during cycle and anestrus. Epithelial cells were moderate to strongly staining for these receptors, with evident irregularity in different types of cells. Apparently ciliated epithelial cells were stained but the intensity was much less than that observed in nonciliated epithelial cells, probably reflecting different functions of these cells. Stromal cells showed moderate staining during dioestrus and strongest reactivity in estrous and anestrus; muscle cells showed strong reactivity for ER throughout the cycle. The reactivity for LHR was weak to moderate throughout the cycle in the epithelial cells and weak in the muscle cells. During anestrus only three strains of muscle cells showed reactivity for LHR. In dioestrus the intensity was strongest. These findings evidence for the firs time the presence for LHR in extra-gonadal reproductive organs of mare. Though there were reports of ER and PR expression in equine endometrium, this is the first report of localization of these receptors in cervix and oviduct of mare using immunohistochemistry. It was found marked individual variation among the strains. These results probably were caused by the variation among the day of cycle and the complexity of mechanisms involved in the presence of these receptors. The findings of the present study allow us to infer that the ovarian steroid hormones nad LH function through their receptors in different tissues of mare reproductive tract, can help us to elaborate new strategies to improve the reproductive efficiency in this specie.
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The Effects of the Female Reproductive Hormones on Ovarian Cancer Initiation and Progression in a Transgenic Mouse Model of the DiseaseLaviolette, Laura January 2011 (has links)
Ovarian cancer is thought to be derived from the ovarian surface epithelium (OSE), but it is often diagnosed during the late stages and therefore the events that contribute to the initiation and progression of ovarian cancer are poorly defined. Epidemiological studies have indicated an association between the female reproductive hormones and ovarian cancer etiology, but the direct effects of 17β-estradiol (E2), progesterone (P4), luteinizing hormone (LH) and follicle stimulating hormone (FSH) on disease pathophysiology are not well understood.
A novel transgenic mouse model of ovarian cancer was generated that utilized the Cre/loxP system to inducibly express the oncogene SV40 large and small T-Antigen in the OSE. The tgCAG-LS-TAg mice developed poorly differentiated ovarian tumours with metastasis and ascites throughout the peritoneal space. Although P4 had no effect; E2 significantly accelerated disease progression in tgCAG-LS-TAg mice. The early onset of ovarian cancer was likely mediated by E2’s ability to increase the areas of putative preneoplastic lesions in the OSE.
E2 also significantly decreased survival time in ovarian cancer cell xenografts. Microarray analysis of the tumours revealed that E2 mainly affects genes involved in angiogenesis and cellular differentiation, proliferation, and migration. These results suggest that E2 acts on the tumour microenvironment in addition to its direct effects on OSE and ovarian cancer cells.
In order to examine the role of the gonadotropins in ovarian cancer progression, the tgCAG-LS-TAg mice were treated with 4-vinylcyclohexene-diepoxide (VCD) to induce menopause. Menopause slowed the progression of ovarian cancer due to a change in the histological subtype from poorly differentiated tumours to Sertoli tumours.
Using a transgenic mouse model, it was shown that E2 accelerated ovarian cancer progression, while P4 had little effect on the disease. Menopause (elevated levels of LH and FSH) altered the histological subtype of the ovarian tumours in the tgCAG-LS-TAg mouse model. These results emphasize the importance of generating animal models to accurately recapitulate human disease and utilizing these models to develop novel prevention and treatment strategies for women with ovarian cancer.
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The control of prolactin secretion and the role of gonadotrophin releasing hormone in the production of concordant secretory spikes of luteinizing hormone and prolactin in the luteal phase of the menstrual cycleKaplan, Hilton January 1988 (has links)
The control of prolactin secretion is a complex interaction of peptides and neurotransmitters acting either in an inhibitory or stimulating way to effect final secretion of this hormone from the lactotrope cell in the anterior hypothalamus. These factors may act either directly on the lactotrope cell or indirectly by changing either dopamine restraint of prolactin secretion or by modulating peptide substances or neurotransmitters higher up in the hypothalamus. Gonadal steroids may also modulate the effect of peptides or dopamine at the level of the lactotrope. Prolactin's major role in the female rat is one of milk production post - partum, nurturing the young. It probably also has other physiological functions and may play a part in the menstrual cycle although this is controversial. Certainly, pulsatile secretion of prolactin during the menstrual cycle is well established and in the luteal phase this is concomitant with the secretion of luteinizing hormone. Theories explaining the synchronous surges seen during this phase of the menstrual cycle have been proposed and GnRH has been implicated in the genesis of the concordance of these secretory spikes. Using a potent GnRH antagonist an experiment was undertaken to establish the role of GnRH by blocking this hypothalamic peptide and observing the effect that this had on luteinizing hormone, prolactin and follicle stimulating hormone. In the first part of the thesis the control of prolactin secretion is reviewed. In the following section, an experiment was performed using a potent GnRH antagonist. A dose response curve was established for the antagonist action on LH. Then a twice maximum dose of this peptide was administered to three subjects in the midluteal phase of the menstrual cycle and the response of LH, prolactin and FSH was measured. The results indicate that although the GnRH antagonist significantly blocked LH secretory peaks, this action was not observed for either prolactin or FSH. This result is perhaps at variance with previous data which suggested that GnRH was responsible for concordant secretory spikes of LH and prolactin in the midluteal phase of the menstrual cycle.
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THE ROLE OF LUTEINIZING HORMONE IN ALZHEIMER DISEASEWebber, Kate M. January 2007 (has links)
No description available.
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