Diss. (sammanfattning) Stockholm : Karol. inst., 2007. / Härtill 3 uppsatser.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2002. / Härtill 4 uppsatser.

Diss. (sammanfattning) Stockholm : Karol. inst., 2003. / Härtill 6 uppsatser. - Titel från omslaget.

Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / "July 1998." Typescript. Vita. Includes bibliographical references (leaves 190-217). Also available on the Internet.

Thesis (Ph.D. in Immunology) -- University of Colorado Denver, 2007. / Typescript. Includes bibliographical references (leaves 133-156). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

Thesis (Ph.D.)--University of Alabama at Birmingham, 2009. / Title from PDF title page (viewed on July 1, 2010). Includes bibliographical references.

Thesis (doctoral)--Katholieke Universiteit te Nijmegen.

Thesis (Ph. D.)--University of Washington, 2000. / Vita. Includes bibliographical references (leaves 139-193).

Des lymphocytes B (LB) appelés LB régulateurs (LBreg) jouent un rôle primordial dans le contrôle de la réponse immunologique.Ces LBreg peuvent être induits suite à une stimulation. Ainsi, le Toll-like receptor (TLR) 9, récepteur de l’immunité innée, participe à cette induction. La voie de signalisation du TLR9 est déficiente chez les patients atteints de lupus érythémateux disséminé qui souffrent d’hyperinflammation chronique et dont les LBreg sont défectueux. Pour étudier le TLR9, des anticorps commerciaux sont classiquement utilisés. Parmi eux, l’anticorps monoclonal de souris anti-TLR9 26C593.2 cible un épitope présent à la membrane plasmique des LB. De plus, cet anticorps active les LB in vitro et bloque l’effet du CpG-ODN, agoniste du TLR9 endosomal. Nous montrons dans ce travail que cinq anticorps anti-TLR9 commerciaux ne sont pas spécifiques du TLR9 et reconnaissent d’autres antigènes. Quatre anticorps sont pourtant capables de reconnaître une protéine TLR9 recombinante commerciale. Afin de contourner le problème de spécificité de ces anticorps, nous avons construit un TLR9 chimérique tagué par une hémagglutinine (TLR9-HA).Trois anticorps reconnaissent cette protéine surexprimée dans des cellules transfectées.Cependant, aucun TLR9-HA ne semble être présent à la membrane plasmique des cellules transfectées. Cette approche nous a permis de démontrer que les anticorps anti-TLR9 se fixent à la membrane plasmique sur une autre cible que le TLR9. Il sera intéressant d’identifier cette protéine afin de mieux comprendre l’effet de l’anticorps anti-TLR9 monoclonal de souris sur l’activation et plus particulièrement sur l’acquisition des propriétés régulatrices des LB. / Regulatory B cells (Breg) play a key role in regulating the immunological response.This regulatory behaviour can be induced by stimulation of the B cells. Toll-like receptor 9 (TLR9), an innate immune receptor, participate to that induction. TLR9 signalling pathway is deficient in B cells from systemic lupus erythematosus patients suffering from chronic hyper-inflammation and having defective Breg cells. To study TLR9, commercial antibodies are commonly used. Among them, the anti-TLR9 monoclonal antibody 26C593.2 targets an epitope located at the plasma membrane of the B cells. Moreover, this particular antibody activates the B cells in vitro and blocks the CpGODN effects, an agonist of the endosomal TLR9. In this work, we show that five commercial antibodies are not specific for the TLR9 protein and recognize other antigens.However, four tested antibodies can recognize recombinant TLR9 molecule. In order to avoid the specificity problem, we achieved to build a chimeric TLR9 protein expressing a hemagglutinin tag at its C-terminal region (TLR9-HA). Three antibodies efficiently target this overexpressed protein in transfected cells.However, no TLR9-HA molecule seems to be located at the plasma membrane of transfected cells. This approach allowed us to demonstrate that anti-TLR9 antibodies recognize another target than TLR9. It would of interest to identify that protein in order to better understand the mouse monoclonal anti-TLR9 antibody effect on B cell activation and Breg properties.

Made available in DSpace on 2014-06-11T19:26:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-10-29Bitstream added on 2014-06-13T19:33:17Z : No. of bitstreams: 1 hata_me_me_jabo.pdf: 405459 bytes, checksum: 3bd879e643e736a926ba7adff70cd7b0 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O medo é uma característica comportamental importante em espécies domesticadas e pode ser incluído no programa de seleção, pois responde à seleção artificial e tem conseqüências importantes ao bem estar e desempenho das aves domésticas. A reação de medo pode ser avaliada pelo tempo de permanência em imobilidade tônica (IT), que é o período em que o animal fica em estado catatônico induzido manualmente pelo homem. Quanto menos tempo permanecer neste estado, menor é o medo do animal e mais adaptado este se mostra a viver em cativeiro. A característica IT é bem representativa do nível de medo do indivíduo e também pode estar relacionada com a relação heterófilo/linfócito (H/L). Assim, o objetivo deste trabalho foi determinar efeitos de ambiente e estimar parâmetros genéticos da característica tempo de permanência em IT de perdiz (Rhynchotus rufescens) criada no ambiente de cativeiro. A análise realizada pelo método dos quadrados mínimos revelou a influência da época de nascimento dentro de geração e o peso corporal, sendo que animais mais pesados permaneceram maior tempo em IT (b = 0,32 ± 0,14g, p<0,05). O método de máxima verossimilhança restrita possibilitou a estimação do coeficiente de herdabilidade da característica IT, apresentando valor igual a 0,29 evidenciando influência do ambiente sobre o tempo de permanência em IT. Contudo, a seleção pode ser eficiente para alterar as médias desta característica / Fear is an important behavior trait in domesticated species and can be included in the selection program, and have important consequences to the welfare and performance of poultry. The fear reaction can be measured by the time spend in tonic immobility (TI), which is the period where the animal stays in catatonic state induced by human hand. The less time remaining in this state, smaller is the fear of the animal and it shows more adapted to living in captivity. The trait IT is well representative of the level of fear of the individual and may also be related to the heterophil to lymphocyte ratio (H/L). The objective of this study was to determine the effects of environment and estimate the genetic characteristic of tonic immobility time in Red-winged Tinamou (Rhynchotus rufescens) hosed in captivity environment. The method of least squares analysis resulted the influence of season within generation and body weight, whose heavier animals showed longer period in IT (b = 0.32 ± 0.14 g, p <0.05). The restricted maximum likelihood method was applied to estimate heritability of TI with value of 0.29 indicating environmental influence However, the selection can be effective to change the means of this trit