Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-05-22T11:14:29Z No. of bitstreams: 1 469172 - Texto Completo.pdf: 823400 bytes, checksum: 095e93ad0abe67b38883346b9108692f (MD5) / Made available in DSpace on 2015-05-22T11:14:29Z (GMT). No. of bitstreams: 1 469172 - Texto Completo.pdf: 823400 bytes, checksum: 095e93ad0abe67b38883346b9108692f (MD5) Previous issue date: 2015-03-25 / Introduction: the objective of this study was to evaluate the temperature variation of the pulp chamber during brackets bonding in different teeth types, with and without primer and in healthy or filled teeth. Materials and Methods: ninety human permanent teeth were used, 30 mandibular incisors, 30 maxillary bicuspid and 30 mandibular third molars, which were divided into 9 groups according to bonding technique (with or without light-curing primer), tooth type, filled or healthy tooth and thickness. A K-type thermocouple connected in a digital thermometer was introduced into the pulp chamber. Periapical radiographs were taken to check the thermocouple positioning and the brackets were bonded using a LED. The initial and maximum temperature reached during light curing were used to calculate the temperature variation. Results: the ANCOVA analysis showed that the bonding technique and tooth type (p?0.05) influenced the temperature variation. There was a greater increase in temperature using primer. Regarding the tooth type, the incisors experienced a major temperature increase, followed by molars and bicuspids. There was no statistically significant difference for healthy or filled teeth groups and thickness factor. Conclusion: a molar showed the lowest temperature variation with 0.2?C and a lower incisor with the greatest variation of 4.3?C. The bonding technique using primer and adhesive caused a greater temperature range compared to the bonding with adhesive only. The mandibular incisor obtained a greater temperature range, then the molar and bicuspid. Therefore, the brackets bonding didn`t exceed the 5,5?C recommended for the pulp health maintenance. Key words: light curing of dental adhesives, thermometers, braces, hot temperature, orthodontics. / ntrodu??o: o objetivo desse estudo foi avaliar a varia??o de temperatura da c?mara pulpar durante a colagem de br?quetes em diferentes tipos de dentes, com e sem utiliza??o de primer e em dentes h?gidos e restaurados. Materiais e M?todos: foram utilizados 90 dentes permanentes humanos, sendo 30 incisivos inferiores, 30 primeiros pr?-molares superiores e 30 terceiros molares inferiores, divididos em 9 grupos de acordo com a t?cnica de colagem, com ou sem primer, tipo de dente e se o dente era h?gido ou restaurado. Um termopar tipo K foi introduzido na c?mara pulpar e conectado ? um term?metro digital. Radiografias periapicais foram realizadas para conferir o posicionamento do termopar no interior da c?mara pulpar e os br?quetes foram colados utilizando um LED. As temperaturas inicial e m?xima atingida durante a fotopolimeriza??o foram utilizadas para calcular a varia??o da temperatura. Resultados: a an?lise de ANCOVA mostrou que a varia??o de temperatura foi influenciada pela t?cnica de colagem e pelo tipo de dente (p?0,05). Utilizando primer houve um maior aumento de temperatura em rela??o ao grupo sem primer. Quanto ao tipo de dente, os incisivos sofreram um maior aumento de temperatura, seguido dos molares e pr?-molares. N?o houve diferen?a estatisticamente significativa para os grupos de dentes h?gidos ou restaurados e para o fator espessura. Conclus?o: o dente que obteve menor varia??o de temperatura foi um molar com 0,2?C e, um incisivo inferior, com a maior varia??o, de 4,3?C. A t?cnica de colagem utilizando primer e adesivo causou uma varia??o de temperatura maior se comparado ? colagem apenas do adesivo. O incisivo inferior obteve uma maior varia??o de temperatura, seguida do molar e pr?-molar. Portanto, a colagem de br?quetes n?o excedeu os 5,5?C preconizados para manuten??o da sa?de pulpar.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-06-15T11:26:51Z No. of bitstreams: 1 468076 - Texto Completo.pdf: 1121444 bytes, checksum: 65fc7c05be36d73bf2d8b7f5853d6a61 (MD5) / Made available in DSpace on 2015-06-15T11:26:51Z (GMT). No. of bitstreams: 1 468076 - Texto Completo.pdf: 1121444 bytes, checksum: 65fc7c05be36d73bf2d8b7f5853d6a61 (MD5) Previous issue date: 2013-11-29 / OBJECTIVE : The objective of this study was to evaluate in 6 months Class V restorations clinically with composite resin ( Glacier ) and glass ionomer cement ( Riva selfcure ) with the direct technique and restorations semidirect technique, cemented with self-adhesive resin cement ( SeT ) and total etching cement ( RelyX ARC ) . MATERIAL AND METHODS : One hundred and twenty restorations were performed by a single operator in 30 patients . Each patient received a composite resin restoration ( RGLA ) , glass ionomer cement ( RVA ) , ceromer cemented with RelyX ARC ( RARC ) , and cemented with SeT (RSET ) . After 6 months, the evaluation was performed using the USPHS criteria for the presence of restoration, marginal integrity , color and presence of secondary caries . RESULTS : The results were obtained through statistical analysis of generalized linear mixed model and showed that only the restorations cemented with SeT were statistical difference , with significant loss of restorations . No patient had secondary caries after 6 months of evaluation . The marginal integrity and color were not significantly different between groups . CONCLUSION : We conclude that restorations cement with self-adhesive SeT failed . Thus the direct restorations had a better behavior than the semidirect restorations. However semidirects restorations cemented with Adper Single Bond 2 + RelyX ARC and total etching presented along with the RVA group the highest predictive average. / OBJETIVO: O Objetivo deste trabalho foi avaliar clinicamente no per?odo de 6 meses restaura??es classe V em resina composta (Glacier) e cimento de ion?mero de vidro (Riva selfcure) pela t?cnica direta, e restaura??es com cer?mero pela t?cnica semidireta cimentadas com cimento resinoso autoadesivo (SeT) e com condicionamento total (RelyX ARC). MATERIAL E M?TODO: Cento e vinte restaura??es foram realizadas por um ?nico operador em 30 pacientes. Cada paciente recebeu uma restaura??o de resina composta (RGLA), Cimento de ion?mero de vidro (RVA), cer?mero cimentada com RelyX ARC (RARC), e cer?mero cimentada com SeT (RSET). Ap?s 6 meses as avalia??es foram realizadas atrav?s dos crit?rios do USPHS modificado quanto ? presen?a da restaura??o, integridade marginal, descolora??o marginal e presen?a de c?rie secund?ria. RESULTADOS: Os resultados foram obtidos atrav?s da an?lise estat?stica de modelo linear misto generalizado e mostraram que somente as restaura??es de cer?mero cimentadas com SeT apresentaram diferen?a estat?stica, com perda significativa das restaura??es. Nenhum paciente apresentou c?rie secund?ria ap?s os 6 meses de avalia??o. A integridade marginal e descolora??o marginal n?o apresentaram diferen?a estat?stica entre os grupos. CONCLUS?O: Conclu?mos que as restaura??es realizadas com cimento autoadesivo SeT falharam. Desta forma as restaura??es diretas tiveram um comportamento melhor que as restaura??es semidiretas. Todavia as restaura??es semidiretas cimentadas com Adper Single Bond 2 + RelyX ARC e condicionamento ?cido total apresentaram junto com o grupo RVA a maior m?dia preditiva.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-06-17T18:27:45Z No. of bitstreams: 1 470532 - Texto Completo.pdf: 1614440 bytes, checksum: e43e90ac45fd2b22f0d595dd9c475e47 (MD5) / Made available in DSpace on 2015-06-17T18:27:45Z (GMT). No. of bitstreams: 1 470532 - Texto Completo.pdf: 1614440 bytes, checksum: e43e90ac45fd2b22f0d595dd9c475e47 (MD5) Previous issue date: 2015-03-04 / This study aimed to evaluate "in vitro" possible changes on prosthetic retaining screws on implants with platform external hexagon, this because the loosening this component is very common in clinical dentistry practice. Were assessed screws of UCLA's pillars of titanium Neodent? brand and 3i BIOMET? and of titanium with surface treated with tungsten carbide Neodent? brand. Two techniques of utilization of the screws were compared, the technique I, recommended by the manufacturer with only the definitive torque, and the technique II , utilized in broad scale in Brazil where multiple torques are applied in the same screw simulating the clinical steps, up to definitive installation. The initial weight of all the screws (T0) was registered, in the screws of technique I, it was given definitive torque (32N.cm to Neodent? and 20N.cm to 3i BIOMET?) submitted to mechanical cycling and the final weight (TF) registered. In the screws of technique II have been applied opening and closing cycles, and their weights registered (T1, T2 and T3) before applying the definitive torque and mechanical cycling after the final weight has been registered (TF). The morphologies and the surface compositions of the screws were evaluated using SEM and EDS at each time. The results showed significant weight loss in the three screws groups, of technique I (p = 0.005 for uncoated Neodent?, p = 0.007 for coated Neodent? and p = 0.001 to 3i BIOMET?) and on two the steps of the three groups of technique II (p <0.001 for all groups), and comparing the final weight of the screws of the two techniques, the screws of the technique I, showed significantly higher values than the screws, of the technique II (p = 0.007 for uncoated Neodent?, p = 0.009 for coated Neodent? and p = 0.001 to 3i BIOMET?), as well as SEM showed less permanent deformation for the technique I. EDS showed no major changes in the surface compositions. Therefore, the use of the definitive screw only at time of final torque can minimize problems regarding to the loosening the retaining screws of implant supported prostheses. / O presente estudo teve como objetivo avaliar ?in vitro? poss?veis altera??es em parafusos de reten??o de pr?teses sobre implantes com plataforma hex?gono externo, isto porque o afrouxamento deste componente ? muito frequente na pr?tica cl?nica odontol?gica. Foram avaliados parafusos de pilares UCLA de tit?nio da marca Neodent? e 3i BIOMET? e de tit?nio com superf?cie tratada com carboneto de tungst?nio da marca Neodent?. Duas t?cnicas de utiliza??o dos parafusos foram comparadas, a t?cnica I, recomendada pelo fabricante com apenas o torque definitivo, e a t?cnica II, utilizada em grande escala no Brasil onde s?o aplicados m?ltiplos torques no mesmo parafuso, simulando as etapas cl?nicas at? a instala??o definitiva. Foi registrado o peso inicial de todos os parafusos (T0), nos parafusos da t?cnica I foi dado torque definitivo (32N.cm para Neodent? e 20N.cm para 3i BIOMET?), submetidos a ciclagem mec?nica e registrado o peso final (TF). Nos parafusos da t?cnica II foram aplicados ciclos de abertura e fechamento e seus pesos registrados (T1, T2 e T3) antes da aplica??o do torque definitivo e ciclagem mec?nica, ap?s o peso final foi registrado (TF). As morfologias e as composi??es de superf?cie dos parafusos foram avaliadas atrav?s de MEV e EDS em cada tempo. Os resultados evidenciaram perda significativa de peso nos tr?s grupos de parafusos da t?cnica I (p=0,005 para Neodent? sem revestimento, p=0,007 para Neodent? com revestimento e p=0,001 para 3i BIOMET?) e em duas das etapas dos tr?s grupos da t?cnica II (p<0,001 para todos os grupos), sendo que comparando o peso final dos parafusos das duas t?cnicas, os parafusos da t?cnica I apresentaram valores significativamente maiores que os parafusos da t?cnica II (p=0,007 para Neodent? sem revestimento, p=0,009 para Neodent? com revestimento e p=0,001 para 3i BIOMET?), assim como a MEV evidenciou menor deforma??o permanente para a t?cnica I. EDS n?o apresentou grandes altera??es nas composi??es de superf?cie. Portanto, a utiliza??o do parafuso definitivo apenas no momento do torque final pode minimizar problemas com rela??o ao afrouxamento de parafusos de reten??o de pr?teses implanto suportadas.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-07-30T17:52:46Z No. of bitstreams: 1 472789 - Texto Completo.pdf: 2619919 bytes, checksum: ed658917522947aaf046eb032864fcc4 (MD5) / Made available in DSpace on 2015-07-30T17:52:46Z (GMT). No. of bitstreams: 1 472789 - Texto Completo.pdf: 2619919 bytes, checksum: ed658917522947aaf046eb032864fcc4 (MD5) Previous issue date: 2015-03-09 / This study aimed to evaluate "in vitro" possible changes on prosthetic retaining screws on implants with platform external hexagon, this because the loosening this component is very common in clinical dentistry practice. Were assessed screws of UCLA's pillars of titanium Neodent? brand and 3i BIOMET? and of titanium with surface treated with tungsten carbide Neodent? brand. Two techniques of utilization of the screws were compared, the technique I, recommended by the manufacturer with only the definitive torque, and the technique II , utilized in broad scale in Brazil where multiple torques are applied in the same screw simulating the clinical steps, up to definitive installation. The initial weight of all the screws (T0) was registered, in the screws of technique I, it was given definitive torque (32N.cm to Neodent? and 20N.cm to 3i BIOMET?) submitted to mechanical cycling and the final weight (TF) registered. In the screws of technique II have been applied opening and closing cycles, and their weights registered (T1, T2 and T3) before applying the definitive torque and mechanical cycling after the final weight has been registered (TF). The morphologies and the surface compositions of the screws were evaluated using SEM and EDS at each time. The results showed significant weight loss in the three screws groups, of technique I (p< 0.005 for uncoated Neodent?, p = 0.001 for coated Neodent? and p < 0.001 to 3i BIOMET?) and on one step of the uncoated Neodent? group and two steps of the other two groups of technique II (p <0.001 for all groups), and comparing the final weight of the screws of the two techniques, only the uncoated Neodent? screws of the technique I, showed significantly higher values than the screws, of the technique II (p = 0.021 for uncoated Neodent?, p = 0.089 for coated Neodent? and p = 0.095 to 3i BIOMET?), as well as SEM showed less permanent deformation for the technique I of these screws. EDS showed no major changes in the surface compositions. Therefore, the use of the definitive screw only at time of final torque would not necessarily minimize problems regarding to the loosening the retaining screws of implant supported prostheses. / O presente estudo teve como objetivo avaliar ?in vitro? poss?veis altera??es em parafusos de reten??o de pr?teses sobre implantes com plataforma hex?gono interno, pois o afrouxamento deste componente ? muito frequente na pr?tica cl?nica odontol?gica. Foram avaliados parafusos de pilares UCLA de tit?nio da marca Neodent? e 3i BIOMET? e de tit?nio com superf?cie tratada com carboneto de tungst?nio da marca Neodent?. Duas t?cnicas de utiliza??o dos parafusos foram comparadas, a t?cnica I, recomendada pelo fabricante com apenas o torque definitivo, e a t?cnica II, procedimento que ? comumente realizado pelos profissionais no Brasil, onde s?o aplicados m?ltiplos torques no mesmo parafuso, simulando as etapas cl?nicas at? a instala??o definitiva. Foi registrado o peso inicial de todos os parafusos (T0), nos parafusos da t?cnica I foi dado torque definitivo (32N.cm para Neodent? e 20N.cm para 3i BIOMET?), submetidos a ciclagem mec?nica e registrado o peso final (TF). Nos parafusos da t?cnica II foram aplicados ciclos de abertura e fechamento e seus pesos registrados (T1, T2 e T3) antes da aplica??o do torque definitivo e ciclagem mec?nica, ap?s o peso final foi registrado (TF). As morfologias e as composi??es de superf?cie dos parafusos foram avaliadas atrav?s de MEV e EDS em cada tempo. Os resultados evidenciaram perda significativa de peso nos tr?s grupos de parafusos da t?cnica I (p<0,001 para Neodent? sem revestimento, p=0,001 para Neodent? com revestimento e p<0,001 para 3i BIOMET?), e em uma das etapas do grupo Neodent sem cobertura e duas etapas dos outros dois grupos da t?cnica II (p<0,001 para todos os grupos), sendo que comparando o peso final dos parafusos das duas t?cnicas, apenas os parafusos Neodent? sem revestimento da t?cnica I apresentaram valores significativamente maiores que os parafusos da t?cnica II, (p=0,021 para Neodent? sem revestimento, p=0,089 para Neodent? com revestimento e p=0,095 para 3i BIOMET?), do mesmo modo a MEV evidenciou menor deforma??o permanente para a t?cnica I desses parafusos. EDS n?o apresentou grandes altera??es nas composi??es de superf?cie. Portanto, a utiliza??o do parafuso definitivo apenas no momento do torque final n?o minimizar? necessariamente problemas com rela??o ao afrouxamento de parafusos de reten??o de pr?teses implanto suportadas.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-08-17T20:23:24Z No. of bitstreams: 1 473528 - Texto Completo.pdf: 1665841 bytes, checksum: 1e03f16b78da931d0d71809bb313ee0d (MD5) / Made available in DSpace on 2015-08-17T20:23:24Z (GMT). No. of bitstreams: 1 473528 - Texto Completo.pdf: 1665841 bytes, checksum: 1e03f16b78da931d0d71809bb313ee0d (MD5) Previous issue date: 2014-08-28 / Resin-modified glass-ionomer cement (RMGIC) is mainly indicated as a liner or base in restorative materials. In deep dentinal cavities where there is not symptomatology, only partial dentine is removed in order to avoid the pulp exposure. Thus, the restorative material is very close to the pulp. The aim of this study was to evaluate the incorporation of zinc oxide or extract of propolis in a RMGIC, subsequently testing the mechanical properties and citotoxicity. For the control group, the RMGIC (Vitremer, 3M ESPE) was handled in its original formulation (G7). Zinc oxide was inserted to the powder of RMGIC in the following proportions: 10% (G1), 20% (G2) e 30% (G3), which were mixed with the original liquid of Vitremer. The extract of propolis was inserted to the liquid of Vitremer in the following proportions: 10% (G4), 20% (G5), 30% (G6), which were mixed with the original powder of Vitremer. Compressive strength, diametral tensile, microhardness and roughness were measured. The cytotoxity was evaluated by MTT assay and neutral red after 24 and 48 hours of exposition. Data were analyzed using the IBM SPSS 22. To verify normality and homogeneity, Shapiro-Wilk and Levene tests were used, respectively, with significance level of 5%. The roughness, microhardness and compressive strength were assessed by one-way ANOVA followed by a multiple-comparison Tukey post hoc test. The diametral tensile was assessed by one-way ANOVA followed by a multiple-comparison Games-Howell post hoc test. For cytotoxity tests, one-way ANOVA and Duncan post hoc test was used to identify intergroup differences. The level of significance was set at p < 0,05. No significant difference was observed in the compressive strength and diametral tensile amog groups in relation to control group (G7). Groups 2, 4 e 6 did not differ in microhardness to the control group (G7). In the roughness test, the group 6 presented the highest mean statistical difference from group 7. Experimental groups showed similar or highest values of cell viability when compared to the group 7 in MTT assay and neutral red tests. The addition of 10% and 20% of extract of propolis or zinc oxide do not interfere in the mechanical proprieties and cytotoxicity of RMGIC. / O cimento de ion?mero de vidro modificado por resina (CIVMR) tem como fun??o principal servir como liner ou base na presen?a de material restaurador ou at? mesmo como material restaurador. Em cavidades profundas com aus?ncia de sinais e sintomas de pulpite irrevers?vel, a dentina afetada por c?rie pode ser mantida a fim de evitar a exposi??o pulpar, permanecendo, assim, um material muito pr?ximo ? polpa. O objetivo deste estudo foi avaliar os efeitos da inclus?o de ?xido de zinco ou de extrato de pr?polis em um CIVMR nas propriedades mec?nicas e citotoxicidade. Para o grupo controle, o cimento de ion?mero de vidro modificado por resina (Vitremer, 3M ESPE) foi manipulado em sua formula??o original (G7). As modifica??es foram realizadas atrav?s da inclus?o de ?xido de zinco ao p? do CIVMR nas seguintes propor??es: 10% (G1), 20% (G2) e 30% (G3) com rela??o ao peso, sendo aglutinado com o l?quido original do material. O extrato de pr?polis foi inserido ao l?quido do Vitremer nas seguintes propor??es: 10% (G4), 20% (G5) e 30% (G6) com rela??o em volume, sendo aglutinado com o p? original do CIVMR. Para a an?lise das propriedades mec?nicas foram realizados os testes de resist?ncia ? tra??o diametral, resist?ncia ? compress?o, microdureza e rugosidade superficial, 24 horas ap?s a confec??o dos corpos de prova. Para a an?lise da citotoxicidade foram realizados os testes de MTT e vermelho neutro, sendo avaliados nos per?odos de 24 e 48 horas. Os dados obtidos foram analisados atrav?s do software IBM SPSS 22. A distribui??o de normalidade foi verificada atrav?s do teste de Shapiro-Wilk e a homogeneidade atrav?s do teste de Levene. Os testes de rugosidade, microdureza e compress?o foram submetidos a ANOVA de um fator e Tukey. O teste de tra??o diametral foi submetido aos testes de ANOVA ucelentem fator e de Games-Howell. Os testes de citotoxicidade foram submetidos aos testes de ANOVA de um fator e Duncan. Todos os testes foram realizados com n?vel de signific?ncia de 5%, considerando p < 0,05. N?o foi registrada diferen?a estat?stica entre os grupos testados nos testes de resist?ncia a compress?o e tra??o diametral com rela??o ao grupo controle (G7). Na microdureza, os grupos 2, 4 e 6 n?o diferiram estatisticamente do grupo 7. Com rela??o ? rugosidade, apenas o grupo 6 apresentou m?dia superior ao G7. Nos testes de MTT e vermelho neutro, todos os grupos mostraram valores similares ou superiores ao grupo 7. A adi??o de extrato de pr?polis ou ?xido de zinco n?o interfere nas propriedades mec?nicas e na citotoxicidade do CIVMR nas concentra??es de at? 20%.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-09-04T19:35:28Z No. of bitstreams: 1 474592 - Texto Completo.pdf: 757600 bytes, checksum: b10e64c161ec1e67c4e8d9aa24a580ae (MD5) / Made available in DSpace on 2015-09-04T19:35:28Z (GMT). No. of bitstreams: 1 474592 - Texto Completo.pdf: 757600 bytes, checksum: b10e64c161ec1e67c4e8d9aa24a580ae (MD5) Previous issue date: 2015-03-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / An appropriate monomer conversion of composite resins is very important to improve your biocompatibility. The aim of this study was to evaluate additional polymerization methods of dental resins cytotoxicity. A total of 72 samples were prepared from the following materials: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). The used color was A2 for all composite resins. To Filtek Z350XT and Sinfony a Teflon mould (3x2mm) was used. From Lava Ultimate block 1,6mm cubes was cutting with diamond discs and digital pachymeter. Samples was divided in 4 groups: FK Group: 20s LED polymerization, S Group: Sinfony with light and vacuum additional polymerization, L Group: Lava Ultimate, FPA Group: Filtek Z350XT with light and vacuum additional polymerization. Evaluate specimens was stored in DMEM medium for 24, 48 and 72 hours respectively. Thereafter, culture medium was in contact with NIH/3T3 fibroblasts. MTT test determined cellular viability. Statistical analysis realized by Levene and Kolmogorov-Smirnov tests (?=0,05). The S group show the lower cytotoxicity. Additional polymerization of FK group wasn?t efficient to cellular viability. Sinfony presented minor cytotoxicity among all tested materials. Light and vacuum additional polymerization of Filtek Z350XT don?t improve your cytotoxicity results when compared with direct polymerization of the same dental composite. / Um satisfat?rio grau de convers?o do mon?mero ? importante para melhorar a biocompatibilidade das resinas compostas. O objetivo deste estudo foi avaliar o efeito dos m?todos de polimeriza??o adicional na citotoxicidade de diferentes comp?sitos restauradores. Para isso foram confeccionadas 18 amostras de cada uma das seguintes resinas testadas: Filtek Z350XT? (3M/ESPE, St Paul, MN, EUA), Sinfony (3M/ESPE, St Paul, MN, EUA), Lava Ultimate (3M/ESPE, St Paul, MN, EUA). A cor usada em todas as reinas foi a A2 de corpo. Para as resinas Filtek Z350XT e Sinfony utilizaram-se moldes de politetrafluoretileno de 3x2mm. Para a resina Lava Ultimate foram cortados do bloco cubos com 1,6mm de face usando um disco diamantado e paqu?metro digital. As amostras foram divididas em 4 grupos: Grupo FK: Filtek Z350XT polimerizada por 20s com LED, Grupo S: Sinfony com polimeriza??o adicional com luz e v?cuo, Grupo L: Lava Ultimate, Grupo FPA: Filtek Z350XT com polimeriza??o adicional com luz e v?cuo. As esp?cies avaliadas estiveram em contato com meio DMEM por um per?odo de incuba??o de 24, 48 e 72 horas respectivamente. Ap?s, o meio de cultura foi colocado em contato com as c?lulas de fibroblastos NIH/3T3. Teste de MTT foi realizado para an?lise da viabilidade celular. Testes de Levene e Kolmogorov-Smirnov (?=0,05) foram utilizados para an?lise estat?stica. O grupo S apresentou-se com a citotoxicidade mais baixa. J? a polimeriza??o adicional no grupo FK n?o mostrou-se eficiente para a viabilidade celular. A resina Sinfony apresentou a menor citotoxicidade dentre os tr?s materiais analisados. Uma polimeriza??o adicional por luz e v?cuo na resina Filtek Z350XT n?o melhora seu resultado para citotoxicidade quando comparado a polimeriza??o direta da mesma.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-02-11T10:24:45Z No. of bitstreams: 1 DIS_TIAGO_KUNZ_COMPLETO.pdf: 2813092 bytes, checksum: 4a31e9fa6f6b00754e7f702733f5c592 (MD5) / Made available in DSpace on 2016-02-11T10:24:45Z (GMT). No. of bitstreams: 1 DIS_TIAGO_KUNZ_COMPLETO.pdf: 2813092 bytes, checksum: 4a31e9fa6f6b00754e7f702733f5c592 (MD5) Previous issue date: 2015-11-30 / Screw loosening is often a complication reported in prosthetic implants. The torque develops a compressive clamping force between parts called preload, which holds the components together and the reducing of friction between the surfaces has been shown to increase its value. Dry lubricants, also called coatings have been used and the results are satisfactory, but the use of liquid lubricants is still little explored, which is the aim of this study. For this, 14 titanium screws and 14 screws with (Diamond-Like Carbon) DLC coating were randomly assigned to four groups (Titanium; DLC, Titanium with oil, and DLC with oil) and subjected to five cycles of tightening and loosening. The screws of groups Titanium with oil and DLC with oil were lubricated with castor oil. The preload was measured by a preload gauge cell, especially developed for the study. The results of preloads and average removal torques for torques of 20 and 32Ncm at 2 different times were analyzed: after the first grip and after five consecutive grips. At the end of the analysis, the thread pitches were measured to rule out plastic deformation. The Group DLC had higher pre-load values at all time points, but it was only significant after the sequence grips with 32 Ncm, and in the absence of oil. It was concluded, therefore, that the castor oil lubrication was not able to significantly increase the preload values or removal torque. / O afrouxamento do parafuso ? uma complica??o frequentemente reportada em pr?teses sobre implantes. O torque desenvolve uma for?a compressiva de aperto entre as partes chamada pr?-carga, que mant?m os componentes unidos, e a redu??o do atrito entre as superf?cies tem demonstrado aumentar o seu valor. Lubrificantes secos, tamb?m chamados de recobrimentos, t?m sido utilizados e os resultados s?o satisfat?rios, contudo o emprego de lubrificantes l?quidos ainda ? pouco explorado, sendo o objetivo deste estudo. Para isso, 14 parafusos de tit?nio e 14 parafusos com recobrimento de Diamond-Like Carbon (DLC) foram aleatoriamente alocados em quatro grupos (Tit?nio; DLC; Tit?nio com ?leo; e DLC com ?leo) e submetidos a cinco ciclos de aperto e desaperto. Os parafusos dos grupos Tit?nio com ?leo e DLC com ?leo foram lubrificados com ?leo de mamona. A pr?-carga foi mensurada por meio de uma c?lula aferidora de pr?-carga, desenvolvida especialmente para o estudo. Foram analisados os resultados das pr?-cargas e torques de remo??o m?dios para os torques de 20 e 32 Ncm em dois momentos distintos: ap?s o primeiro aperto e depois de cinco apertos consecutivos. Ao fim das an?lises, os passos de roscas foram mensurados para descartar deforma??es pl?sticas. O Grupo DLC apresentou maiores valores de pr?-carga em todos os momentos de avalia??o, contudo ele s? foi significativo ap?s a sequ?ncia de apertos com 32 Ncm, e na aus?ncia do ?leo. Concluiu-se, portanto, que a lubrifica??o com ?leo de mamona n?o foi capaz de aumentar significativamente os valores da pr?-carga ou do torque de remo??o.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-02-12T17:20:18Z No. of bitstreams: 1 TES_MAGDA_DE_SOUSA_REIS_COMPLETO.pdf: 2952696 bytes, checksum: 9a8e4368b83491c5888a26990b0c9ddc (MD5) / Made available in DSpace on 2016-02-12T17:20:18Z (GMT). No. of bitstreams: 1 TES_MAGDA_DE_SOUSA_REIS_COMPLETO.pdf: 2952696 bytes, checksum: 9a8e4368b83491c5888a26990b0c9ddc (MD5) Previous issue date: 2015-10-15 / The aim of this study was to evaluate dental pulp and furcal periodontal tissue responses to mineral trioxide aggregate (MTA) and to a calcium silicate-based material (Biodentine?). The lower first molars in hundred forty (140) male Wistar rats were divided into groups according to the experimental site and period (14 and 21 days) testing two materials (n=8 per group/period in pulpotomy and n=6 per group/period in furcal perforation). Groups capped and sealed with MTA or Biodentine? were restored with silver amalgam; in an additional test group, teeth were capped and restored with Biodentine?. Teeth capped with gutta-percha and restored with silver amalgam served as a positive control and untouched teeth were negative control. Histological evaluation of pulp responses and macroscopic examination of coronal sealing were performed. Inflammation extension and intensity, degenerative events and formation of mineralized tissue barrier were assessed. In the experiment of furcal perforations, inflammation extension and intensity, bone resorption and cement formation were assessed histologically. All results underwent ANOVA and Tukey post hoc tests (p<.05). In pulpotomy, Biodentine? and MTA presented satisfactory results, which were characterized by a mild inflammatory response (p<.0001) and more pronounced formation of mineralized barrier (p<.0001) compared to teeth capped with gutta-percha. When used as a restorative material, Biodentine? kept coronal sealing in only 37.5% of the samples. As to furcal perforations, Biodentine? and MTA presented satisfactory results, which were characterized by a milder inflammatory response compared to the control, regardless of the material used for coronal sealing and of the experimental period evaluated (p<.0001). All test groups showed less bone resorption than the positive control after 21 days (p<.05), being this difference more pronounced in teeth restored with silver amalgam. Cement repair occurred in 30% of MTA and Biodentine? samples, and was not detected in any specimen of the positive control group. In conclusion, Biodentine? and MTA promoted similar responses when used to pulp capping and furcal perforation sealing. On the other hand, the use of Biodentine? as temporary restorative material did not promote efficient coronal sealing. Although further investigations are required, Biodentine? can be considered an alternative for pulpotomy and furcal perfuration. / O objetivo deste estudo foi comparar a resposta tecidual, ap?s pulpotomias e perfura??es de furca, utilizando agregado tri?xido de mineral (MTA) e o material ? base de silicato tric?lcico (Biodentine?). Os primeiros molares inferiores de 140 ratos Wistar machos foram utilizados em dois tempos (14 e 21 dias) e divididos em grupos conforme o experimento e materiais utilizados (pulpotomia n=8 e perfura??o de furca n=6). Nos dois experimentos, grupos capeados e selados com MTA e Biodentine? foram restaurados com am?lgama de prata; um grupo adicional utilizou somente Biodentine? e o controle positivo recebeu guta-percha e restaura??o de am?lgama; dentes intactos serviram de controle negativo. No experimento de pulpotomias, realizou-se exame macrosc?pico e radiogr?fico do selamento coron?rio e exame histol?gico dos eventos inflamat?rios (intensidade e extens?o), degenerativos e de forma??o de barreira mineralizada. No experimento de perfura??es de furca, foram avaliados histologicamente os eventos inflamat?rios (intensidade e extens?o), de reabsor??o ?ssea e reparo de cemento. Os dados foram comparados atrav?s da an?lise de vari?ncia (ANOVA) e posthoc de Tukey (p<.05). Nas pulpotomias, o Biodentine? e MTA apresentaram resultados satisfat?rios, com menor resposta inflamat?ria (p<.0001) e pronunciada forma??o de barreira mineralizada (p<.0001) comparada aos dentes do controle positivo. O Biodentine?, utilizado como restaura??o, manteve o selamento coron?rio em apenas 37.5% das amostras. Nas perfura??es de furca, o Biodentine e MTA apresentaram resultados satisfat?rios, caracterizados por uma resposta inflamat?ria mais suave em compara??o ao controle positivo, independentemente do material utilizado para o selamento coron?rio e per?odo experimental avaliado (p<.0001). Todos os grupos testes mostraram menor reabsor??o ?ssea do que o controle positivo ap?s 21 dias (p<.05), sendo esta diferen?a mais acentuada em dentes restaurados com am?lgama de prata. A repara??o de cemento ocorreu em 30% das amostras de MTA e Biodentine, e n?o foi detectada em qualquer amostra do grupo do controle positivo. Concluiu-se que houve respostas similares ap?s o uso de Biodentine? e MTA no capeamento da polpa exposta e selamento de perfura??es de furca. Por outro lado, o uso do Biodentine? como material restaurador provis?rio n?o promoveu selamento coron?rio eficiente. Embora sejam necess?rias novas investiga??es, o Biodentine deve ser considerado como uma alternativa frente aos tratamentos propostos.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-04-12T14:08:12Z No. of bitstreams: 1 DIS_VANIA_STEPHANIE_SANCHEZ_GAMARRA_PARCIAL.pdf: 192614 bytes, checksum: ac0bcd8326b2183c17301f29601b81ee (MD5) / Made available in DSpace on 2016-04-12T14:08:12Z (GMT). No. of bitstreams: 1 DIS_VANIA_STEPHANIE_SANCHEZ_GAMARRA_PARCIAL.pdf: 192614 bytes, checksum: ac0bcd8326b2183c17301f29601b81ee (MD5) Previous issue date: 2016-01-25 / Conselho Nacional de Pesquisa e Desenvolvimento Cient?fico e Tecnol?gico - CNPq / The aim of this study was to evaluate, in vitro, the marginal adaptation and microleakage of class II restorations of SonicFill composite resin with different curing techniques. Class II preparations were prepared in 40 human premolars (mesial margin 1 mm below the CEJ, distal margin 1 mm above the CEJ), and randomly divided into 4 groups according to curing technique (n = 10 per group): G1: 1200 mW/cm2 for 20 s; G2: 1200 mW/cm2 for 40 s; G3: Soft-start for 20 s (650 mW/cm2 for 5 s, increasing to 1200 mW/cm2 for 15 s); G4: Soft-start for 40 s (650 mW/cm2 for 10 s, increasing to 1200 mW/cm2 for 30 s). Three-step etch&rinse adhesive Optibond FL was applied and the cavity was filled in bulk with SonicFill composite resin, followed by oclusal sculpture and photoactivation. Impressions were made using polyvinyl siloxane and epoxy resin replicas were obtained, before and after thermocycling. The occlusal and cervical margins were analyzed with SEM at 200x magnification. The marginal adaptation was expressed as percentages of continuous margins. After thermocycled, the specimens were immersed in silver nitrate 50%, and analyzed the extention of dye penetration in the cervical-mesial and cervical-distal margins. According to ANOVA and Tukey?s test, there was statistical difference in the percentage of continuous margins on the occlusal-palatine for group 1 (83.19%), differing statistically from groups 2, 3 and 4 that had values over 95% for continuous margins (p?0,05). In the cervical margin there was no statistical difference between the groups (p?0,05). According to Student's t test, after thermocycling, there was a statistical significant reduction of percentage of continuous margins in occlusal-buccal margin for groups 1, 2 and 4, as well as in the cervical-mesial margin for groups 1, 3 and 4, and cervical-distal margin only to group 1. According to Kruskal-Wallis test, there was no significant difference in the microleakage scores between groups in the enamel (p=0.373) and in dentin (p=0.561), being predominantly score 1 in enamel and score 3 in dentin. The bulk filling technique with SonicFill composite resin associated with three-step etch&rinse adhesive Optibond FL in class II cavities provided gap formation and microleakage, especially in the cervical dentin region, regardless of the curing technique. / O objetivo do estudo foi avaliar, in vitro, a adapta??o e o selamento marginal de restaura??es classe II em resina composta SonicFill com diferentes t?cnicas de fotoativa??o. Foram realizados preparos classe II em 40 pr?-molares humanos (caixa mesial 1 mm abaixo da JCE, caixa distal 1 mm acima JCE), sendo divididos aleatoriamente em 4 grupos de acordo com a t?cnica de fotoativa??o (n = 10 por grupo): G1: 1200 mW/cm2 por 20 s; G2: 1200 mW/cm2 por 40 s; G3: Soft-start por 20 s (650 mW/cm2 por 5 s, elevando para 1200 mW/cm2 por 15 s); G4: Soft-start por 40 s (650 mW/cm2 por 10 s, elevando para 1200 mW/cm2 por 30 s). O sistema adesivo Optibond FL foi aplicado e a cavidade foi preenchida em incremento ?nico com a resina composta SonicFill, seguido da escultura da oclusal e fotoativa??o. A partir de moldagens com silicone por adi??o, foram obtidas r?plicas em resina ep?xica das restaura??es antes e ap?s termociclagem. As margens das r?plicas, ao n?vel oclusal e cervical, foram analisadas no MEV em 200x, sendo determinada a porcentagem de margens continuas. Ap?s termociclagem, os corpos de prova foram submetidos ? metodologia de microinfiltra??o com nitrato de prata a 50%, sendo analisada a extens?o da penetra??o na margem cervical mesial e distal. De acordo com ANOVA e teste de Tukey, houve diferen?a estat?stica na porcentagem de margens cont?nuas na oclusal-palatina para o grupo 1 (83,19%), diferindo estatisticamente dos grupos 2, 3 e 4 que tiveram valores acima de 95% para margens cont?nuas (p?0,05). Na margem cervical n?o houve diferen?a estat?stica entre os grupos (p?0,05). De acordo com o teste t-student, ap?s termociclagem, houve redu??o estatisticamente significativa na porcentagem de margens continuas na margem oclusal-vestibular para os grupos 1, 2 e 4, assim como na margem cervical-mesial para os grupos 1, 3 e 4, e na regi?o cervical-distal apenas para o grupo 1. De acordo com o teste de Kruskal-Wallis, n?o houve diferen?a significativa para os escores de microinfiltra??o entre os grupos, tanto no esmalte (p=0,373) como na dentina (p=0,561), sendo predominantemente escore 1 em esmalte e escore 3 em dentina. A t?cnica de incremento ?nico com a resina composta SonicFill, associada ao sistema adesivo Optibond FL, em cavidades classe II, proporcionou a forma??o de fendas e a ocorr?ncia de microinfiltra??o, principalmente na regi?o cervical com t?rmino em dentina, independente da t?cnica de fotoativa??o.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-05-06T16:53:23Z No. of bitstreams: 1 DIS_LETICIA_SPINELLI_JACOBY_PARCIAL.pdf: 409701 bytes, checksum: ece1c9e62d9b0d680c9b4a7d64a27079 (MD5) / Made available in DSpace on 2016-05-06T16:53:23Z (GMT). No. of bitstreams: 1 DIS_LETICIA_SPINELLI_JACOBY_PARCIAL.pdf: 409701 bytes, checksum: ece1c9e62d9b0d680c9b4a7d64a27079 (MD5) Previous issue date: 2016-02-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Introduction: The aims of this study were to assess the in vitro cytotoxicity of orthodontic bands, with and without silver solder, and to compare the viability of the HaCaT, HGF, MRC-5, and Vero cell lines, when exposed to orthodontic band extracts. Material and Methods: An MTT assay was performed on the extracts of the tested materials. The culture medium alone was used as a negative control and copper strip extracts were used as a positive control. Results: A one-way Analysis of Variance (ANOVA) showed a difference between all of the groups in all of the cell lines and Tukey?s post-hoc test revealed no significant differences between the stainless steel orthodontic bands and the negative control group. There was a significant difference between the negative control group and the stainless steel orthodontic bands with silver solder joints in the HaCaT and MRC-5 cell lines. A great variability in the percentages of cell viability was observed. According to these results, the stainless steel orthodontic bands did not induce cytotoxic effects on any of the cell lines. When associated with silver solder, the orthodontic bands decreased the cell viability in the HaCaT, HGF, MRC-5, and Vero cell lines, but there was only a statistical significance in the HaCaT and MRC-5 cell lines. Conclusions: More than one type of cell line must be tested in a cytotoxicity assay due to the outcome variability from one cell line to another. / Introdu??o: Os objetivos deste estudo foram avaliar, in vitro, a citotoxicidade dos an?is ortod?nticos com e sem soldagem ? prata e comparar a viabilidade celular nas linhagens FGH, HaCaT, MRC-5 e Vero ap?s a exposi??o aos extratos dos an?is ortod?nticos. Materiais e M?todos: Foi realizado o ensaio de redu??o de MTT ap?s a exposi??o das culturas celulares aos extratos dos materiais testados. Foram utilizados como controles negativo e positivo, respectivamente, meio de cultura DMEM e extratos de tira de cobre. Resultados: A an?lise de vari?ncia de uma via (ANOVA) mostrou que houve diferen?a estatisticamente significativa entre os grupos em todas as linhagens avaliadas. O teste de compara??es m?ltiplas de Tukey detectou aus?ncia de diferen?a significativa entre os grupos controle negativo e an?is ortod?nticos de a?o inoxid?vel. Houve diferen?a estatisticamente significativa entre os grupos controle negativo e an?is ortod?nticos de a?o inoxid?vel com soldagem ? prata nas linhagens celulares HaCaT e MRC-5. Observou-se grande variabilidade nas percentagens de viabilidade celular entre as linhagens. Conclus?es: Ao avaliar a citotoxicidade de an?is ortod?nticos com e sem soldagem ? prata, existe variabilidade nos resultados quando linhagens diferentes s?o comparadas. An?is ortod?nticos de a?o inoxid?vel n?o induziram efeitos citot?xicos em nenhuma das linhagens testadas. Quando associados ? solda de prata, os extratos dos an?is ortod?nticos reduziram a viabilidade celular nas linhagens FGH, HaCaT, MRC-5 e Vero, embora essa redu??o tenha ocorrido de forma estatisticamente significativa apenas nas linhagens HaCaT e MRC-5.