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L'échocardiographie est la modalité d'imagerie la plus utilisée pour évaluer la morphologie et la fonction cardiaque. Il s'agit d'un outil non invasif pour le diagnostic et l'évaluation des maladies cardiaques et il permet en outre de surveiller la réponse au traitement. Cependant, la quantification des événements cardiaques rapides demeure un défi avec la cadence d’imagerie actuellement réalisable, en particulier dans des applications telles que l'échocardiographie d'effort. De plus, cette limitation devient plus prononcée en imagerie 3D conventionnelle focalisée en raison du temps nécessaire pour insonifier et acquérir un volume complet. Le fait que l’on puisse actuellement atteindre au mieux ~20 volumes par seconde est l'une des raisons qui limitent son utilisation courante dans la pratique clinique. Des améliorations dans ce domaine permettraient d'exploiter l'important potentiel de l'imagerie 3D pour la quantification complète de la déformation cardiaque.Dans ce contexte, l'objectif de cette thèse était de développer des méthodes à haute cadence d'images et de tester leur performance dans des conditions réalistes visant la prise de décision pour une transition vers la pratique clinique. Pour atteindre cet objectif, des expériences in vitro et in vivo ont été menées en utilisant l'imagerie 2D et 3D. Notre première contribution a été une comparaison 2D entre deux modalités à haute cadence d'images en termes de qualité d'image et de performance d'estimation de mouvement. Motivés par nos résultats 2D mais surtout par le défi d'implémenter le MLT dans la pratique, nous avons étendu cette approche en 3D. Nous avons étudié la faisabilité de la MLT 3D dans des conditions statiques et dynamiques. Enfin, comme l’évaluation de nouvelles approches dans des conditions physiologiques de flux complexes constitue un pas en avant vers la transition clinique, notre troisième contribution a consisté à valider des modalités 2D et 3D à haut cadence d'images sur un fantôme du vortex / Echocardiography is the most widely used imaging modality for assessing cardiac morphology and function. It does provide a non-invasive tool in diagnosis and assessment of heart diseases and it allows, in addition, monitoring the response to the treatment. However, quantifying fast cardiac events remains a challenge when using the current achievable frame rate, especially in applications such as stress-echocardiography. Moreover, this limitation becomes more pronounced in 3D conventional focused imaging due to the time needed to insonify and acquire a full volume. The fact that only ~20 volumes per second can currently be achieved is one of the reasons restricting its common usage in clinical practice. Improvements in this field would allow exploiting the important potential of 3D imaging in providing a full quantification of cardiac deformation.In this context, the aim of this thesis was to develop high frame rate methods and to test their performance in realistic conditions aiming decision making towards clinical translation. To achieve this objective, both in vitro and in vivo experiments were conducted using 2D and 3D imaging. Our first contribution was a 2D comparison between two high frame rate modalities in terms of image quality and motion estimation performance. Motivated by our 2D results but especially by the challenge of implementing MLT in practice, we extended this approach to 3D. We studied the feasibility of 3D MLT in both static and dynamic conditions. Finally, as testing novel approaches in physiological complex flows conditions is a step forward towards clinical translation, our third contribution was to validate 2D and 3D high frame rate modalities on a ring vortex phantom

Chlamydia trachomatis is an obligate intracellular bacterium of major human health concern, causing urogential chlamydia infections, lymphogranuloma venereum (LGV) and trachoma. Chlamydia is one of the most common sexually transmitted infections worldwide and can cause infertility. In the first four papers described herein we used a high resolution multilocus sequence typing (MLST) system to investigate the epidemiology of C. trachomatis, and showed that MLST is superior to conventional ompA genotyping with respect to resolution. In the fifth paper we simplified the methodology by developing and validating a multilocus typing (MLT) DNA microarray based on the MLST system. In more detail, MLST analysis of consecutive specimens from 2006 in Örebro County in Sweden, and comparison to specimens from 1999-2000, showed that the new variant C. trachomatis (nvCT) is monoclonal and likely has appeared in recent years. MLST analysis of LGV specimens from men who have sex with men (MSM) showed that the increase of LGV in Europe in the last decade indeed was a clonal outbreak, contrary to the USA where LGV might have been present all along. In the third paper, clinical symptoms could not be correlated with the MLST genotypes, suggesting, together with the combined results of all previous studies, that bacterial factors, if important, need to be understood in the context of host factors. MLST analysis of specimens from a high incidence C. trachomatis area in North Norway revealed interesting epidemiological details concerning unusual genetic variants, the nvCT and MSM, but found no significant difference in genetic diversity compared to two other geographic areas in Norway. Lastly, we developed a MLT array that provides high resolution while being rapid and cost-effective, which makes it an interesting alternative for C. trachomatis genotyping. In conclusion, the MLST system and the MLT array have proven to be useful tools and should now be applied in further investigations to improve our understanding of C. trachomatis epidemiology.

This Master thesis examines a phenomenon that occurs in the upper polar atmosphere, namely, Polar Mesospheric Winter Echoes, or PMWEs. PMWEs are radar echoes observed by Very High Frequency Mesosphere Stratosphere Troposphere (VHF MST) radar, from altitudes of 60 to 76 km at 7 to 15 UT, in the winter months from the middle of September to the beginning of May. The aforementioned specifications are the partial results of this thesis.PMWEs are generally understood to be caused by turbulence; however, radar data indicate some rare cases where PMWEs can be created with velocities exceeding the speed of sound, which is not possible with current turbulence theory. Kirkwood et al., 2006 and Belova, Kirkwood, and Sergienko, 2013 hypothesised that infrasound could generate the necessary conditions for PMWEs with velocities equal to or exceeding the speed of sound. Observations of PMWEs presented in this thesis have been carried out by the MST radar ESRAD, located at Esrange (67 56’N, 21 04’E) near Kiruna in northern Sweden. The radar operates at 52 MHz and has been performing continuous radar observations since Dec 1996. Observations of the infrasounds presented in this thesis were carried out by a microbarometer located close to Rymdcampus in Kiruna. Access to the data is restricted, but through university administration, this master’s thesis has been granted permission to use the data for the study of PMWEs. The instrument performed continuous infrasound measurements from the 24th of May 2016 and forward. This thesis will perform a full analysis of all radar data from the 17th of Dec 1996 until the 31st of Jan 2021 to assess the altitude interval, diurnal interval and yearly interval. The data given in the first section are a result of this work. Additionally, space weather parameters relation with the occurrence of PMWEs is analysed. Space weather parameters are very important for the chemistry and conditions present in the mesosphere. It is shown thatt here is a strong relation between solar wind and PMWE occurrence, decent relation with Kp index and no to weak relation with solar particle event (SPE). Correlating space weather and PMWEs in greater detail could be the subject of other studies. Last but most interesting, microbarometer data will be analysed for days where high-speed PMWEs are detected. Because of the rarity of these high-speed PMWEs, only seven total cases were found from 24 May 2016 onward, making the analysis of the infrasound measurements very limited, and no connection was found. However, it was found that days with high-speed PMWEs had an abnormally low amount of infrasound detections, further making analysis difficult but raising questions of why. This could mean that infrasound signals might be hindered from reaching the ground on days where we have high-speed PMWEs. No further conclusions can be made, as this indicates relation but not causation. Airborne infrasound instruments could be used to detect weaker infrasound signals due to being unaffected by wind disturbance at the ground.