Mon travail de thèse porte sur l’étude du rôle de l’histone acétyl-transférase MOZ et de l’histone méthyle-transférase MLL dans l’hématopoïèse. Elles contrôlent l’expression de nombreux gènes, nottament des gènes HOX, des facteurs de transcription connus pour leur rôle dans l’hématopoïèse normale et pathologique. Les deux protéines ont des gènes cibles communs tel qu'HOXA9. Ces observations nous ont conduit à rechercher une coopération fonctionnelle entre MOZ et MLL. Nous avons montré que MOZ était associée avec MLL dans les cellules souches/progénitrices humaines CD34+ afin d’activer la transcription des gènes HOXA5, HOXA7 et HOXA9. En effet, les deux protéines interagissent et sont recrutées au niveau de leur promoteur. Nous avons mis en évidence une interférence fonctionnelle entre ces deux facteurs épigénétiques, puisque MOZ est nécessaire au recrutement et à l’activité enzymatique de MLL au niveau des gènes HOXA5, HOXA7 et HOXA9 et réciproquement.Afin de caractériser le mécanisme d’action impliquant la coopération entre MOZ et MLL, nous avons recherché d’autres partenaires associés à ce duo. Nous avons identifié la Symplekin, un membre de la machinerie de polyadénylation. Nous avons mis en évidence l’interaction de la Symplekin avec MOZ et MLL dans les cellules de la lignée hématopoïétique humaine KG1. Les trois protéines sont co-recrutées sur le promoteur du gène HOXA9. Nous avons démontré le rôle ambivalent de la Symplekin. Bien qu’elle soit importante pour la polyadénylation et par conséquent pour la stabilité de l’ARN Hoxa9, la Symplekin empêche le recrutement de MOZ et de MLL au niveau du gène HOXA9, conduisant ainsi à une diminution de sa transcription. / My thesis project has consisted of the study of MOZ, and MLL. They are epigenetic regulators. MOZ and MLL activate transcription of HOX genes, which are transcription factors essential during haematopoiesis. MOZ and MLL have some target genes in common. In our study, we characterised a cooperation between MOZ and MLL in human haematopoietic stem/progenitor cells CD34+. They are both recruited onto HOX promoters. MOZ is essential for MLL recruitment, and this is reciprocal. In conclusion, we provided an example of a mechanism involving a direct cross-talk between two histone modifying enzymes.In order to dissect the mechanism of action of this complex, we decided to identify novel proteins interacting with both MOZ and MLL. A member of the RNA polyadenylation machinery has been isolated: Symplekin. We confirmed the interaction between MOZ, MLL and Symplekin in the human haematopoietic immature cell line KG1. We showed that Symplekin is co-recruited to HOXA9 promoter along with MOZ and MLL. We demonstrated the dual role of this member of the polyadenylation machinery. Indeed, besides the fact that Symplekin is important for Hoxa9 polyadenylation, thus its stability, it prevents MOZ and MLL recruitment onto HOXA9 promoter, leading to a decrease of HOXA9 transcription.Our work improved the understanding of the mechanism of action of MOZ and MLL in HOX control.

Submitted by Ana Rosa Silva (arosa@ufpa.br) on 2012-09-10T14:59:54Z No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_TensõesUsoPropriacao.pdf: 2478224 bytes, checksum: d86a65c4b6dc0f523e0f9f10adec116e (MD5) / Approved for entry into archive by Ana Rosa Silva(arosa@ufpa.br) on 2012-09-10T15:23:29Z (GMT) No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_TensõesUsoPropriacao.pdf: 2478224 bytes, checksum: d86a65c4b6dc0f523e0f9f10adec116e (MD5) / Made available in DSpace on 2012-09-10T15:23:29Z (GMT). No. of bitstreams: 2 license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Dissertacao_TensõesUsoPropriacao.pdf: 2478224 bytes, checksum: d86a65c4b6dc0f523e0f9f10adec116e (MD5) Previous issue date: 2011 / SEDUC/PA - Secretaria de Estado de Educação / The peasants' struggle for land in the Xingu at the turn of the twentieth century is the focus of this work. We will deal with forms of organization and struggle of the peasantry within the global/local dynamic that led to the creation of the extractive reserve "Verde para Sempre" in the municipality of Porto de Moz, state of Pará. It was in memory of social groups and through the research field that we seek the peasant perspective of development, and its actions and strategies to remain in their territories against the advancing of big business to the field from the 1960s in the Amazon. / A luta dos camponeses pela terra no Xingu na virada do século XXI é o foco deste trabalho. Trataremos das formas de organização e luta do campesinato dentro da dinâmica global/local que desembocou na criação da RESEX “Verde para Sempre”, no município de Porto de Moz, no Estado do Pará. É na memória dos grupos sociais e por meio da pesquisa de campo que se buscou a perspectiva camponesa de desenvolvimento, e suas ações e estratégias para permanência em seus territórios diante do avanço do grande capital para o campo a partir da década de 1960 na Amazônia. / SEDUC/PA - Secretaria de Estado de Educação

Submitted by Cássio da Cruz Nogueira (cassionogueirakk@gmail.com) on 2017-02-20T14:51:41Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PoliticasPublicasDesenvolvimento.pdf: 9486497 bytes, checksum: 6caa73df7d05b3a26d0bbb4246279354 (MD5) / Approved for entry into archive by Edisangela Bastos (edisangela@ufpa.br) on 2017-03-02T11:46:24Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PoliticasPublicasDesenvolvimento.pdf: 9486497 bytes, checksum: 6caa73df7d05b3a26d0bbb4246279354 (MD5) / Made available in DSpace on 2017-03-02T11:46:25Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Tese_PoliticasPublicasDesenvolvimento.pdf: 9486497 bytes, checksum: 6caa73df7d05b3a26d0bbb4246279354 (MD5) Previous issue date: 2014-07-14 / A presente tese de doutoramento constitui o resultado das atividades de investigação realizada nas Reserva Extrativista Verde para Sempre e Arióca Pruanã – PA, com o objetivo de estabelecer entendimento sobre as consequências produzidas pela criação das duas Unidades de Conservação a partir de aspectos socioeducacionais e ambientais. A pesquisa é do tipo etnográfico, com base de interpretação qualitativa. Fez-se uso de estudo de caso comparativo, levantamentos bibliográficos, pesquisas de campo e entrevistas. Com base nesses procedimentos, descreveram-se os desdobramentos gerados pela criação da RESEX tendo com referência as políticas públicas educacionais já previstas, bem como a legislação ambiental vigente. A pesquisa considerou as transformações provocadas na dinâmica das relações sócio-políticas, econômicas e ambientais dos municípios de Porto de Moz e Oeiras do Pará, bem como a capacidade das políticas públicas vigentes com vistas a contemplar as necessidades e perspectivas das populações que vivem dentro e no entorno da unidade de conservação. Os resultados apontaram a fragilidade na implementação de políticas públicas nas duas UC, inexistência de elementos que estabeleçam relação entre o cotidiano vivido nas RESEX e o currículo escolar, ausência de projetos educativos formais e não formais relacionados às Unidades de Conservação no cenário amazônico. / This thesis is the result of research activities carried out in the Extractive Reserves “Verde para Sempre” and “Arióca Pruanã” in the state of Pará, aiming to establish understanding of the consequences produced by the creation of two protected areas from socio-educational and environmental aspects. The research is an ethnographic one, based on qualitative interpretation. Comparative case study, literature surveys, field surveys and interviews were used in this research. Based on these procedures, it has been described the developments generated by the creation of extractive reserves with reference to the educational public policies already planned, as well as environmental laws. The research considered the changes wrought in the dynamics of socio-political, economic and environmental relations in the towns of Porto de Moz and Oeiras do Pará, and the ability of existing public policies in order to address the needs and perspectives of people living in and surrounding the protected area. The results showed the weakness in the implementation of public policies at both protected area, lack of evidence to establish the relationship between the life on the extractive reserves and the school curriculum, lack of formal and non-formal educational projects related to protected area in the Amazon scenario.