A região N-terminal de OTI-VIT-1 foi expressa e os polipeptídeos recombinantes foram purificados. OTI-VIT-1 pode ser homólogo da vitelina YP170B de C. elegans. Foram identificados um intron na região 5´ e dois na região 3´ do gene Oti-vit-1. Antissoro monoespecífico para PVIT1HisC confirmou que o gene Oti-vit-1 codifica VT1. O polipeptídeo recombinante P40-H, correspondente à região N-terminal da proteína OTI-VIT-6 interage com um polipeptídeo de aproximadamente 100 kDa (P100) presente em extratos proteicos totais de O. tipulae. Estudamos também o papel da Proteína Microssômica Transportadora de Triglicerídeos (MTP) na biossíntese de Vitelogenina do nematoide C. elegans. Ensaios de RNAi em C. elegans, utilizando parte da sequência do gene da MTP (Cel-dsc-4) foram realizados nas linhagens N2 e DH1033. Microscopia de fluorescência de vermes adultos da linhagem DH1033, submetidos a RNAi, mostrou acúmulo de YP170B::GFP no interior dos enterócitos. Este acúmulo sugere a participação da MTP na secreção de VTG. Análise imunológica da vitelogenina nestes mesmos vermes não detectaram alterações no processamento de CEL-VIT-6, sugerindo que o mesmo ocorra não só no pseudoceloma, mas também no interior dos enterócitos. / The N-terminal region of OTI-VIT-1 was expressed and the recombinant polypeptides were purified. OTI-VIT-1 may be homologous to the vitellin YP170B from C. elegans. We identified an intron in the 5 \'region and two in 3\' region from Oti-vit-1. Monospecific antisera to PVIT1HisC confirmed that the gene Oti-vit-1 encodes VT1. The recombinant polypeptide P40-H, corresponding to the N-terminal region of the protein OTI-VIT-6, interacts with a polypeptide of approximately 100 kDa (P100) present in total protein extracts of O. tipulae. The role of microsomal triglyceride transfer protein (MTP) in the biosynthesis of vitellogenin was studied in the nematode C. elegans. Trials of RNAi in C. elegans, using the sequence of the MTP gene (Cel-dsc-4) were performed in the strains N2 and DH1033. Fluorescence microscopy of adult worms of strain DH1033, subjected to RNAi, showed accumulation of YP170B:: GFP within the enterocytes. This accumulation suggests the involvement of MTP in the secretion of VTG. Analysis using anti-vitellogenin immune serum did not detect changes in the processing of CEL-VIT-6, suggesting that it occurs not only in pseudocoelom but also within the enterocytes.

Bakalauro baigiamojo darbo tema – Mokslo ir technologijų parkų indėlis Lietuvos konkurencingumui Tikslas – išanalizuoti esamą Lietuvos mokslo ir technologijų parkų (toliau vadinamų MTP) situaciją ir atskleisti būdus, kaip pakeisti Lietuvos MTP aplinkos lygį, kuris padėtų didinti Lietuvos konkurencingumą. Pagrindiniai darbo uždaviniai yra: • Pateikti teorinių žinių apie nacionalinį konkurencingumą; • Pateikti informaciją apie mokslo ir technologijų parkus teoriniame lygmenyje; • Atskleisti užsienio šalių MTP bei šalies konkurencingumo sąsajas – iš ko mes galėtumėme pasimokyti? • Pristatyti Lietuvos MTP, pateikti jų esamą situaciją, veiklą, problemas; • Palyginti Lietuvos ir užsienio mokslo ir technologijų parkus; • Įvardinti konkrečias Lietuvos MTP problemines sritis, remiantis užsienio šalių praktika; • Pateikti pasiūlymus ir rekomendacijas įvardintoms problemoms spręsti. Tyrimo metodika – Lietuvos ir užsienio šalių mokslo ir technologijų parkų palyginamoji analizė. Darbo rezultatuose atsispindės esama Lietuvos MTP padėtis ir probleminės sritys. Stiprus atsilikimas nuo užsienio šalių MTP yra jaučiamas tokiuose kriterijuose kaip bendradarbiavime su universitetais, pakankamos infrastruktūros ir pritaikytų patalpų trūkumas, tikslinės specializacijos nebuvimas ir papildomų paslaugų (mokymų, seminarų, konsultacijų) stoka. Bus pateikti pasiūlymai ir rekomendacijos probleminėms sritims spręsti, ir kaip jas išsprendus būtų galima didinti Lietuvos konkurencingumą. / Bachelor thesis theme – Science and technology park (STP) input on Lithuanian competitiveness. The purpose – to analyze existing Lithuanian science and technology park situation and reveal the means of how to improve Lithuanian STP background, which would help to heighten Lithuanian competitiveness. The essential tasks of this paper are: • Analyze the theory of competitiveness; • Introduce science and technology park theoretical background; • Reveal STP relations with competitiveness; • Introduce Lithuanian science and technology parks, present the existing situation, activities, problems; • Compare Lithuanian and foreign technology parks; • Present Lithuania specific STP problems according to foreign practice; • Present suggestions and recommendations for solving specific Lithuanian technology park problems. Analysis method – comparable method, which shows the differences between Lithuanian and foreign country STP. The results of bachelor thesis will reflect the current Lithuanian STP situation and its problems. The Lithuanian STP is lacking far behind its foreign counterparts in criteria such as communication with universities, insufficient infrastructure and adequate accommodation, lack of objective specialization and additional service (training, seminar, and consultation). In order to solve the underlying problems, suggestions and recommendations will be made. Additional suggestions will be supplied for how to heighten Lithuanian competitiveness after the problems have... [to full text]

This work is an introduction to the GSM system. Gives basic information about its structure, active elements, used protocols and signaling messages. It gives a propsal for structures for emulator of telephone exchange.

The first Metatarsophalangeal (MTP) joint is essential for foot biomechanics and weight-bearing activities. Osteoarthritis in this joint can lead to pain, discomfort, and limited mobility. In order to treat this, Episurf Medical is working to produce individualized implants based on 3D segmentations of the joint. As manual segmentations are both time- and cost-consuming, and susceptible to human errors, automatic approaches are preferred. This thesis uses U-Net and DeepEdit as deep-learning based methods for segmentation of the MTP joint, with the latter being evaluated with and without user interactions. The dataset used in this study consisted of 38 CT images, where each model was trained on 30 images, and the remaining images were used as a test set. The final models were evaluated and compared with regards to the Dice Similarity Coefficient (DSC), precision, and recall. The U-Net model achieved DSC 0.944, precision 0.961, and recall 0.929. The automatic DeepEdit approach obtained DSC of 0.861, precision of 0.842, and recall of 0.891, while the interactive DeepEdit approach resulted in DSC of 0.918, precision of 0.912, and recall of 0.928. All pairwise comparisons in terms of precision and DSC showed significant differences (p<0.05), where U-Net had the highest performance, while the difference in recall was not found to be significant (p>0.05) for any comparison. The lower performances of DeepEdit compared to U-Net could be due to lower spatial resolution in the segmentations. Nevertheless, DeepEdit remains a promising method, and further investigations of unexplored areas could be addressed as future work. / Den första Metatarsalphalangeal(MTP) leden är viktig för fotens biomekanik och viktbärande aktiviteter. Artros i denna led kan leda till smärta, obehag och begränsad rörlighet. För att behandla detta arbetar Episurf Medical med att producera individanpassade implantat baserat på 3D segmenteringar av leden. Då manuella segmenteringar både är tids- och kostnadskrävande, samt känsliga för mänskliga fel, föredras automatiska metoder. Denna avhandling använder U-Net och DeepEdit som djupinlärningsbaserade metoder för segm- entering av MTP leden, varav det senare utvärderas med och utan användarint- eraktion. Datasetet som användes i denna studie bestod av 38 CT bilder, där varje modell tränades på 30 bilder och de återstående användes som testdata. De slutliga modellerna utvärderades och jämfördes med avseende på Dice Similarity Coefficient (DSC), precision och recall. U-Net modellen uppnådde DSC 0.944, precision 0.961 och recall 0.929. Den automatiska DeepEdit metoden erhöll DSC 0.861, precision 0.842 och recall 0.891, medan den interaktiva DeepEdit metoden resulterade i DSC 0.918, precision 0.912 och recall 0.928. Alla parvisa jämförelser avseende precision och DSC visade signifikanta skillnader (p<0.05), där U-Net hade den högsta prestandan, medan skillnaden i recall inte visade sig vara signifikant (p>0.05) för någon jämförelse. Den lägre prestandan för DeepEdit jämfört med U-Net kan bero på lägre spatiell upplösning i segmenteringarna. Dock är DeepEdit fortfarande en lovande metod, och ytterligare undersökningar av outforskade områden kan tas upp som framtida arbete.

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This thesis attempts to provide basic overview of the topic of the GSM system and tries to design Mobile Switching Centre Emulator that could perform the operation Location Update. The first part of this thesis describes the principles and specifics of the GSM network (network structure, the operations required to support user mobility, network entities, etc.). The second part of this thesis describes the SS7 signaling protocols, SIGTRAN and MAP protocol. The final part deals with design of the emulator.

PLC-system har funnits ute i industrin sedan sent 1970-tal och appliceras på miljontals olika processer. Företag inom processindustrin står idag inför stora utmaningar då en instabil marknad ställer krav på en mer hållbar och flexibel infrastruktur. Krav på ökad produktion, bättre kvalité och lägre kostnader har fått fler företag att försöka gå över till modulära automationssystem. Syftet med detta examensarbete är att undersöka applicerbarheten av Module Type Package (MTP) på Cytivas UNICORN-styrsystem och därefter ta fram en arbetsmetod samt eventuell mjukvara som gör detta möjligt. Arbetet valdes att utföras på Cytivas nya automationsplattform för UNICORN™-styrsystemet genom praktisk undersökning av instrumentkonfigurationer på en specifik grupp av deras bioprocesssystem. Med specifikationer och datablad som blev tillförsedda från de tekniska organen NAMUR och VDI/VDE som utvecklar MTP undersöktes en instrumentkonfiguration med fokus på struktur och uppbyggnad. Utifrån dessa undersökningar designades och utvecklades en mjukvara som kunde ta fram MTP-kompatibla data för validering och test med mjukvaruverktyg utvecklade av COPADATA. Arbetet resulterade i slutsatsen att MTP är möjligt att applicera på Cytivas bioprocesssystem med ny automationsplattform. Fortsatt arbete krävs för att ta fram en arbetsmetod samt att mjukvara i framtiden bör utvecklas vidare. / PLC systems have existed in the industry since the late 1970s and are applied worldwide to millions of different processes. Companies in the process industry today face major challenges as an unstable market demands a more sustainable and flexible infrastructure. Demands for increased production, better quality and lower costs have caused more companies to try to switch to modular automation systems.  The aim of this thesis is to examine the applicability of Module Type Package (MTP) on Cytiva's UNICORN control system and then develop a working method and possible software that makes this possible. With specifications and data sheets supplied from the technical bodies NAMUR and VDI/VDE, an instrument configuration was investigated with a focus on its structure. Based on these investigations a software was designed and developed that could produce MTP-compatible data for validation and testing using software tools developed by COPADATA.  The work resulted in the conclusion that MTP is possible to apply to Cytiva's bioprocess systems with their new automation platform. Continued work is required to develop a working method, and that the software that was created should be further developed and improved.

This thesis presents basic structure and entities of mobile network GSM. It presents principles of signalling with focus on core network via signalling systems SS7 and SIGTRAN. Chosen protocol layers and signalling message types are presented. The thesis is concluded by the design and implementation of the application emulating home location register of mobile users.

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal.

Thèse numérisée par la Division de la gestion de documents et des archives de l'Université de Montréal