The purpose of this study was to analyze and compare the agonistic behaviors and reconciliation in captive and free-ranging Formosan macaques (Macaca cyclopis). The dominance style of Formosan macaques was compared with long-tailed, rhesus and Japanese macaques. I have used scan, focal sampling and ad libitum on aggressions of adult macaques. I have recorded post-conflict (PC) focal samplings on victims and compared those with matched control (MC) focal samplings. Agonistic behaviors had significantly higher frequency in captive than in free-ranging Formosan macaques. The frequencies of hostile and submission were significantly higher in captive than in free-ranging Formosan macaques. The captive adult females of higher rank had higher frequency of threat and hostile, and lower frequency of submission. Threat was the most frequent aggression (52-72%) expressed by both the captive and free-ranging adult monkeys. The victims in captive and free-ranging Formosan macaques usually submitted immediately after aggression (82-89%). The proportion of counter aggression in captive and free-ranging Formosan macaques were relative low (9-16%). The aqerage conciliatory tendency for adult Formosan macaques was 14.3% to 19.6%. The affiliative contacts in PC and MC in captive and free-ranging Formosan macaques were striking that both preferred grooming. The Formosan macaques significantly reconciled more during PC than MC period both in captive and free-ranging conditions. In addition, both had significantly more attracted than dispersed PC-MC pairs. The conciliatory tendencies in captive and free-ranging Formosan macaques were similar regardless of kin and non-kin partners. This study indicated that Formosan macaques were close to the macaques of Fascicularis group. Therefore, Formosan macaques had a despotic dominance style as suggested by Phylogenetic hypotheses.

Cynomolgus macaques (Macaca fascicularis) are the most commonly used non-human primate for research and testing in Europe. Their principal use is in preclinical safety testing of new pharmaceuticals to assess risk of adverse effects, as indicated by changes in a core battery of physiological measures before human exposure. Regulatory studies are strictly controlled through legislation and codes of practices underpinned by the principles of humane science, the 3Rs; Replacement, Reduction and Refinement. Despite the link between good welfare and good science now universally made in codes of practice, legislation and the literature, there are few studies aimed at systematically examining the link and almost no quantitative data from cynomolgus macaques used for toxicology. The main aim of this thesis was to examine the link between Refinement, animal welfare and scientific output for this important animal model, piggy-backing on regulatory studies conducted by a large contract research organisation. In the laboratory, animal welfare is formally considered in terms of Refinement which has evolved to include both the reduction of negative welfare states and the proactive enhancement of positive welfare over the animal’s lifetime. A multidisciplinary approach to welfare assessment including measures of behaviour, physiology and physical health, and which built upon current unit procedures was undertaken to produce an overall assessment of welfare in cynomolgus macaques. Macaque facial expressions, vocalisations, activity and position in the home cage, body weight change, body condition and alopecia scores were found to be reliable indicators of welfare state and would be most feasible for care staff to monitor. The concept of quality of scientific output was defined in relation to toxicological findings and includes sensitivity, reliability and repeatability of individual measures in the core battery (e.g. heart rate, blood pressure, haematology, clinical chemistry and organ weights). The link between welfare and quality of scientific output was then systematically explored with Refinements to macaque use in regulatory studies. The first, a data mining study, undertaken to quantify the effects on biological data recorded from cynomolgus macaques, used in regulatory studies over an eight-year period as the CASE sponsor transitioned from single to permanent group housing, found the effects to be highly variable on individual parameters in the core battery and in some instances welfare-positive effects of group housing were confounded by concurrent changes in standard operating procedures. A further study of planned Refinements to macaque-care staff interaction through enhanced socialisation was found to help animals cope better with husbandry and scientific procedures and enhance quality of cardiovascular measures recorded at baseline. In light of these findings a number of recommendations are made including a framework of terms useful for measuring quality of scientific output, a welfare assessment framework and Refinements to husbandry and scientific procedures for cynomolgus macaques used in regulatory toxicology. Because of their capacity to suffer it is both ethically and scientifically important that macaque welfare is maximised and their use results in valid and reliable experimental outcomes informing on the safety and efficacy of new pharmaceuticals prior to human exposure.

Self-injurious behavior (SIB) is a complex phenotype that occurs with an increasing prevalence of about 7-34% in humans and 10-12% in non-human primates (NHPs). This study evaluated the efficacy of probiotic Lactobacillus reuteri as a treatment for self-injurious behavior (SIB) and sleep disruption in rhesus macaques. The treatment was proposed to alleviate mild self-biting, sleep disruption, and reduce chronically elevated hypothalamic-pituitary-adrenocortical (HPA) axis activity, all hallmark features of monkeys with this condition. The probiotic preparation included two strains of L. reuteri (L. reuteri ATCC PTA 6475 & L. reuteri DSM 17938) containing on average 200 million colony forming units per chewable tablet. The study was conducted on 14 rhesus macaque monkeys (9 males) housed at the University of Massachusetts Amherst. To our knowledge this is the first time that a Lactobacillus strain has been used as a treatment for SIB in rhesus macaques. This study utilizes motion-activated infrared camera technology, modified enzyme-immunosorbent-assays (EIAs) techniques to measure hair cortisol concentrations, and daily behavioral observations to provide an overall assessment of the behavioral, physiological, and sleep associated implications of probiotic treatment on SIB and control non-human primates (NHPs). Administration of L reuteri modestly decreased biting behavior in monkeys with SIB (F(2,12) = 5.64, p= 0.02) and showed overall decrease in nighttime activity across all subjects but did not normalize SIB to nonSIB values. Hair cortisol values are pending. These findings and the findings of previous work further strengthen the argument for probiotics as an efficacious treatment for SIB behavior.

No description available.

Le propos de ce travail de thèse est d’examiner le développement de la formation de catégories de visages, par l’étude des préférences visuelles des nourrissons dans la première année de vie. Nous avons cherché à préciser les mécanismes de formation des préférences visuelles en les intégrant dans le cadre théorique développé par Valentine (1991), le face-space. Nous avons proposé de lier ces préférences à la manière dont l’expérience perceptive des nourrissons avec différentes catégories de visages va structurer l’espace de représentation des visages. De manière générale, nous avons postulé que les nourrissons présenteront des préférences pour les visages proches de la tendance centrale (i.e., prototype) du face-space. Nous avons mis en évidence une tendance des nourrissons de 0 à 6 mois à présenter un biais pour des visages d’adultes par rapport à des visages de nourrissons (Etudes 1 et 2), les premiers correspondant à une catégorie de visages prépondérante de l’environnement des nourrissons, là où les seconds correspondent à une catégorie de visages peu rencontrée. Ce biais pour la familiarité s’est avéré disparaitre à 9 et 12 mois (Etude 3). Ces préférences liées à la familiarité pourraient être liées à une forme de fausse reconnaissance du visage des proches des nourrissons, issue de la surreprésentation de ces visages dans le quotidien des nourrissons. Ce pattern de préférences n’a en revanche pas été retrouvé lorsque des nourrissons de 3 à 12 mois ont été confrontés à des visages d’enfants ou de nourrissons (Etudes 4 et 5), les résultats montrant plutôt une préférence pour les visages les moins familiers, relativement à l’expérience des nourrissons. Nous avons ensuite étudié les capacités de catégorisation de nourrissons de 9 et 12 mois pour des visages de différentes catégories d’âges, i.e., adulte, enfant, nourrisson (Etude 6). Les nourrissons de 12 mois ont formé des catégories discrètes des visages d’adulte et de nourrissons d’une part, et d’enfants et de nourrissons d’autre part. Les nourrissons de 9 mois en revanche ont montré un pattern plus asymétrique en ce qu’ils ont formé une représentation des visages d’enfants excluant un nouveau visage de nourrisson, et une représentation des visages de nourrissons incluant un nouveau visage d’enfant. Les nourrissons ayant tous une expérience de la crèche, donc des visages de nourrissons, cette asymétrie pourrait être liée à une influence de la connaissance de cette catégorie de visage. Dans une dernière étude (Etude 7) nous avons cherché à montrer plus directement le lien entre préférences visuelles et proximité par rapport au prototype, chez des nourrissons humains de 12 mois et des nourrissons macaques de 3 mois (Macaca mulatta). La mise en évidence de préférences liées à la distance par rapport au prototype chez ces deux populations suggère la présence d’un mécanisme commun aux deux espèces conduisant à la formation de préférences visuelles pour les visages. / The purpose of this work is to examine of the development of face category formation using infants’ visual preferences. We investigated the mechanisms leading to differential face preferences by integrating them in the theoretical framework developed by Valentine (1991), the face-space. We proposed that the way perceptual experience shape the structure of the face-space is a determinant of infants’ face preferences. We postulated that faces close to the central tendency of the face-space (i.e., prototype) will be preferred. We first reported a bias to look more toward adult faces than infant faces from birth to 6 month of age (Studies 1 and 2). Adult faces correspond to a frequently encountered category while infant faces represent a less frequently encountered category. We also showed a downturn of this familiarity bias as infants grow older (Study 3). The preferences showed by younger infants might be linked to a form of false recognition of the caregivers’ faces, due to the massive exposure to these faces. This pattern of preferences was not found in 3-to 12-month-olds presented with child and infant faces (Studies 4 and 5). Conversely, infants showed a tendency to prefer the less familiar faces, depending on their perceptual experience. We then studied 9- and 12-month-olds’ abilities to form categories of faces differing by age, i.e., adult, child, and infant faces, (Study 6). Twelve-month-olds formed discrete categories of adult and infant faces in one hand, and of child and infants faces on the other hand. Nine month-olds showed an asymmetric pattern of behavior, forming categories of child faces that exclude a new infant face, and categories of infant faces that include a new child face. All these infants being exposed to infant faces via nursery, the asymmetry might stem from the influence of the knowledge of this category of faces. In the last study (Study 7), we tried to provide more direct evidences of the link between face preferences and the distance from the prototype in two different populations: 12-month-old human infants, and 3-month-old macaque infants (Macaca mulatta). Preferences for faces close to the prototype in both species suggest a common mechanism leading to face preferences.

Operant conditioning using positive reinforcement techniques has been used extensively in the management of nonhuman primates in both zoological and laboratory settings. Based on a large body of previous research that demonstrates the utility of such techniques in reducing stress, abnormal behavior, and aggression, this research project was intended to develop and test the usefulness of habituation and counter-conditioning techniques in reducing the fear-responses of singly-housed male rhesus macaques living in the laboratory environment. Additionally, we investigated the variable of temperament as it relates to the reduction of fear-responsivity and overall training success. Based on a Wilcoxon Matched-Pairs Sign Test, we found that animals exposed to desensitization training were significantly likely to show a reduction in the rate at which they engaged in cringing toward humans (exact significance = .016, one-tailed, N ties = 6), cringing in general (exact significance = .016, one-tailed, N ties = 6), and in stress-related behaviors (exact significance = .016, one-tailed, N ties = 6). Animals exposed to basic husbandry training or exposed to no training at all were not significantly likely to show a reduction in the rates of these behaviors. When these same behaviors were analyzed in terms of duration of behavior, desensitization-exposed animals were significantly likely to show reduction in the amount of time spent cringing toward humans (exact significance = .016, one-tailed, N ties = 6), but not in cringing behaviors in general or in stress-related behaviors. Neither the husbandry-exposed group nor the group exposed to no training showed a significant number of subjects exhibiting a reduction in duration of any of these behaviors. Additionally, initial temperament assessments were found to significantly predict the relative ability of subjects exposed to training to acquire trained behaviors such that animals generally ranked as more inhibited in terms of temperament also ranked as slower learners based on a Wilcoxon Matched-Pairs Signed-Ranks test, z = -.316, p = .752 (two-tailed). Results of this study could enhance both laboratory animal welfare and laboratory animal research, and could be a first step in developing techniques for reducing fearful behavior in rhesus monkeys in the laboratory environment.

This essay describes and analyzes of locomotion, sexual, afiliativní and agonistic behavior in a group of pigs macaque (macaca macaques) at Prague Zoo. Data collection took place in the period from December 2015 to May 2016. In the winter months (December to March) at the times from 9.00 to 16.00 hrs., And in April and May 2016 in time from 9.00 to 18.00 pm. The total observation period reached 240 hours. Data were analyzed using Statistics 12th and MS Excel 2007. Objectives of the work are three: (1) Based on the research literature to describe the behavioral ecology of macaque monkeys. (2) The detailed description and analysis of locomotion, sexual, afiliativní and agonistic behavior in a group of macaque pigs in the Prague Zoo. (3) Describe grooming, depending on time of day and social position in the group of pigs macaque in the Prague Zoo. We tested five hypotheses, which can be divided into four categories. We first investigated the hierarchy of the group. Furthermore, we investigated the behavior depending on the sex of the individual, and consequently the position in the hierarchy. In the last part we dealt with in detail during grooming. Tests confirmed the existence of a clear linear hierarchy based on agonistic interactions. The results showed that move around the enclosure depends on...

The development of a safe and effective HIV vaccine remains challenging due to its high antigenic variability. Poxviruses are large, stable, and have a track record of use as human vaccine candidates. Recombinant fowlpox virus 9 (rFP9), a highly attenuated host range-restricted poxvirus strain, has been safely administered to humans with no ill effects, and is known to be immunogenic. This thesis describes the construction of complex rFP9 encoding various sequences of SIVmac239. The SIVmac239/macaque model is widely used for HIV vaccine development. The ultimate aim of this work was to combine the advantages of FP9 with those of live attenuated SIV to produce a safe yet hopefully effective model HIV vaccine candidate. Transfer plasmids for five different insertion sites within the FP9 genome were designed and constructed. Homologous recombination (HR) of adjacent FP9 sequences was employed to facilitate the integration of SIVmac239 sequences into the FP9 genome. Positive rFP9 were identified by blue colouration in presence of X-gal using a transient colour selection (TCS) technique, and the final markerless pure recombinants were confirmed by PCR. Expression of the target SIV proteins in the presence of T7 polymerase has been demonstrated by immunocytochemical (ICC) staining and Western blotting (WB) assays. Expression was also quantified by enzyme-linked immunosorbent assay (ELISA) in various cell lines at multiple time points. Five different unique rFP9 have been constructed through this project. All SIVmac239 open reading frames (ORFs) save nef have been integrated into the FP9 genome, and protein expression demonstrated where possible. Moreover, a single rFP9 vector expressing the defective SIVmac239 genome driven by T7 RNA polymerase has been successfully constructed and validated using a green fluorescent protein marker.rFP9 showed appropriate transgene expression in both avian and mammalian cells, although at different levels. The expression efficiency of rFP9 was finally compared to another attenuated poxvirus vector, modified vaccinia Ankara (MVA). Comparing the protein expression levels between rFP9 and rMVA was quite difficult because different poxvirus promoters (early/late in rFP9; intermediate in rMVA) were used to direct the transcription of the T7 RNA gene. Given this limitation, although generally higher levels of expression were seen with rFP9, this cannot be attributed to the FP9 with any certainty.

No description available.

Natal dispersal may have considerable social, ecological and evolutionary consequences. While speciesspecific dispersal strategies have received much attention, individual variation in dispersal decisions and its fitness consequences remain poorly understood. We investigated causes and consequences of natal dispersal age in rhesus macaques (Macaca mulatta), a species with male dispersal. Using long-term demographic and genetic data from a semi-free ranging population on Cayo Santiago, Puerto Rico, we analysed how the social environment such as maternal family, group and population characteristics affected the age at which males leave their natal group. While natal dispersal age was unrelated to most measures of group or population structure, our study confirmed earlier findings that sons of high-ranking mothers dispersed later than sons of low-ranking ones. Natal dispersal age did not affect males\\\'' subsequent survival, but males dispersing later were more likely to reproduce. Late dispersers were likely to start reproducing while still residing in their natal group, frequently produced extra-group offspring before natal dispersal and subsequently dispersed to the group in which they had fathered offspring more likely than expected. Hence, the timing of natal dispersal was affected by maternal rank and influenced male reproduction, which, in turn affected which group males dispersed to.