This thesis deals with the problem of the high-speed induction machines. In the introduction the design of the machines and problem areas are discussed. The feeding from the frequency converter and the mechanical strength of the rotor appear to be the biggest problem. High-speed engines with solid rotors were designed. The first model is equipped with a conductive cooper layer on the surface. This model is exposed to harmonic and non-harmonic input voltage and then the results of the simulations are compared. The following model of the thesis is equipped with axial slots. In the practical part of the thesis no load measurement is taken. One point was measured at nominal frequency. At the end of the thesis the results of the measurements are compared with the results of the figures extracted from Maxwell software.

The projected retirement home is located in the cadaster of the town Velká Bíteš. The building is designed with respect of resident's needs of retirement age. It is a four-storey building without basement. There are designed external doctor's offices on the first floor. There are lounge with library and technical facilities on the first floor as well. Besides that, we can find accomodation spaces from second up to fourth floor. The structural system is a longitudinal wall and ceilings are made by monolithic reinforced concrete. The roof is shed. The building is detached with built chambers

This master‘s thesis deals with the design documentation Fit4You sports center. The new building is located in the district of the City of Brno in the cadastral Brno – Líšeň. The building is designed as a reinforced concrete skeleton system with ceramic masonry infill with two floors. Part of the building with the sports hall is roofed with arched trusses made of laminated wood. The second part of the building with facilities for the hall is roofed with a flat floor.

Diploma thesis Multifunctional building is compiled in the form of project documentation. The building is located in Brno, Vinařská street. It´s a building with four overground floors designed primarily to housing and one underground floor, which is mainly used as collective garage. The building will be made from POROTHERM blocks with the mineral heat insulation. The ceilings will be made of reinforced concrete. The building has a single-layer flat roof.

El presente proyecto de investigación es Crematto Helado y nació a partir de querer desmentir la relación común entre alimentos saludables y un gasto elevado. La idea de Crematto Helado vende prácticamente la idea de un helado artesanal de frutos exóticos que cuenten con un sabor inusual en un helado y, además, cuente con altas propiedades nutricionales. El producto será ofrecido al mercado en un precio competitivo con una entrega de manera rápida y eficiente. Los ingresos del proyecto Crematto Helado se obtendrán por medio de la venta online de Helados Artesanales y los costos principales son los recursos tales como los envases de vidrio, fruta y mano de obra. Para desarrollar, de manera exitosa, el presente proyecto, establecimos como socios clave principales a los proveedores de fruta y envases de vidrio. El modelo de negocio de Crematto Helado está dirigido a hombres y mujeres de entre 18 a 35 años con un Nivel Socio Económico A y B, con un estilo de vida moderno, sofisticado y adaptado, quienes claramente cuentan con buenos hábitos alimenticios y algunas preferencias por lo eco-amigable. La falta de postres de frutos exóticos bajos de azúcar y la creciente demanda y responsabilidad ambiental de los consumidores nos permitió encontrar un segmento muy llamativo e interesante. En el Concierge, tras publicar en las redes sociales los sabores que se ofrecerían, distintos seguidores de Crematto Helado, denominados hoy “Cremattolovers”, comenzaron a comentar y compartir la publicación. Fue entonces que establecimos nuestra misión el brindar la mejor opción de un helado saludable de frutas exóticas y visión el ser referencia de marca a nivel nacional reconocidos por nuestros helados de sabores no convencionales. El precio final determinado por frasco de helado saludable es de 15 soles, el cual es relativamente menor al de la competencia de cualquier naturaleza; sin embargo, en cuanto a sabores, no llevamos ventaja, ya que Crematto Helado cuenta con solo cinco. Tras establecer objetivos por área decidimos nuestra estrategia genérica, el cual es de Diferenciación porque su característica principal es el ser una alternativa de helado de sabores exóticos. / The present research project is Crematto and it came from wanting to disprove the common relationship between healthy foods and high spending. The idea of Crematto is practically sell the idea of an artisan ice cream with exotic fruits that have an unusual flavor in an ice cream and, in addition, have high nutritional properties. The product will be offered to the market at a competitive price with a fast and efficient delivery. The income from the Crematto project will be obtained through the online sale of Artisan Ice Creams and the main costs are resources such as glass containers, fruit, and labor. To successfully develop this project, we established as main key partners the suppliers of fruit and glass containers. Crematto business model is aimed at people between 18 and 35 years old with a Socio-Economic Level A and B, with a modern, sophisticated, and adapted lifestyle, who clearly have good eating habits and some preferences for eco-friendly products. The lack of low-sugar exotic fruit desserts and the increasing demand and environmental responsibility of consumers allowed us to find an extremely attractive and interesting segment. At the Concierge, after posting the flavors that would be offered on social media, different followers of Crematto, today called “Cremattolovers,” began to comment and share the publication. Then we established our mission to provide the best option for a healthy exotic fruit ice cream and vision to be a national brand reference recognized for our ice cream with unconventional flavors. The last price determined per jar of healthy ice cream is 15 soles, which is less than that of the competition of any nature; However, in terms of flavors, we are not ahead, since Crematto has only five. After establishing objectives by area, we decided on our generic strategy, which is Differentiation because its main characteristic is that it is an alternative to ice cream with exotic flavors. / Trabajo de investigación

Heutzutage werden bereits klein- bis mittelformatige Fassadenplatten aus Textilbeton mit Flächen bis zu 6 m² im Bauwesen verwendet. Dabei werden die ca. 25 mm dicken Elemente mit netzartigen technischen Textilien aus AR-Glas bewehrt. In einem kürzlich abgeschlossenen Forschungsprojekt wurde eine großformatige Fassadenplatte aus Textilbeton mit einer Elementfläche von 12,2 m² entwickelt, die eine Plattendicke von nur 30 mm aufweist. Die Platte wurde mit zwei rückseitigen monolithisch verbundenen Betonstegen ausgesteift. Durch die Beschichtung der Textilien mit Epoxidharz konnten formstabile und formbare Bewehrungsstrukturen entwickelt werden, die Textilspannungen von bis zu 1400 N/mm² aufweisen. In einem Pilotprojekt wurden die Ergebnisse des Forschungsprojektes angewendet und ihre Praxistauglichkeit nachgewiesen. Der Artikel beschreibt das architektonische Konzept, gibt Hinweise zur konstruktiven Durchbildung und beschreibt Herstellverfahren der textilen Bewehrung und Fassadenplatte.

The present work demonstrates how a peptide-based material can be obtained for the biosorptive recovery of metals from contaminated industrial wastewater. Starting with Phage surface display for the initial identification and optimization of gallium-binding peptides, all the following application-focussed experiments are based on chemically synthesized peptides. Two chromatography-based biopanning methods for the identification of gallium-binding peptides from a commercial phage display library were developed. Five gallium-binding peptide sequences were identified and evaluated to show good gallium-binding properties. Furthermore, the biosorption of free gallium and arsenic by gallium-binding bacteriophage clones was investigated. A large influence of the pH-value on the respective interactions was demonstrated. Mutagenesis experiments were also carried out for a bacteriophage clone expressed peptide, in which a cysteine pair systematically replaced amino acids. Biosorption experiments with the resulting seven different bacteriophage mutants suggested a relationship between the rigidity of the peptide structure and the gallium-binding properties. In isothermal titration experiments, the thermodynamics of the interaction between gallium and the peptides as chemically synthesized derivatives were characterized, independent of the bacteriophage. The peptides differed strongly in their interaction with gallium, and in some cases, the complex formation with gallium depended strongly on the surrounding buffer conditions. The peptide with the amino acid sequence NYLPHQSSSPSR has particularly promising gallium-binding properties. Computer modeling suggests the probable structure of the peptide in aqueous solution and postulates a possible binding site for gallium. The side-selective and covalent immobilization of the peptides on a polystyrene matrix led to the creation of a biocomposite for the biosorptive recovery of gallium. The sorption performance and desorbability of the peptide-based biosorption materials were determined in studies with model solutions and real waters from the semiconductor industry.  :EIDESSTATTLICHE VERSICHERUNG II SUMMARY 7 CHAPTER I. 8 Utility of biotechnological approaches in resource technology 9 Phage Surface Display for the recovery of inorganic binding peptides 14 Gallium – Example of a high-tech metal 22 Aims and context of the present work 22 CHAPTER II. 25 Author contributions 25 Abstract 25 Introduction 26 Materials and Methods 28 2.1 Phage Display Library system 28 2.2 Biopanning experiments 29 2.3 Single clone identification 31 2.4 Single clone binding studies 31 Results 32 3.1 Immobilization of gallium ions 32 3.2 Biopanning experiments 33 3.3 Single clone binding studies 38 Discussion 39 4.1 Gallium ions as biopanning target 39 4.2 Phage clone selection 40 Conclusion 44 Acknowledgements 45 CHAPTER III. 46 Author contributions 46 Abstract 47 Introduction 47 Materials and Methods 49 2.1 Handling of phage display library clones 49 2.2 Site-directed mutagenesis experiments 50 2.3 Biosorption experiments 51 Results and Discussion 52 3.1 Experimental context 52 3.2 Original phage clone characterization 53 3.3 Site-directed mutagenesis experiments 56 3.4 Mutant phage clone characterization 57 Conclusions 59 Acknowledgements 60 CHAPTER IV. 61 Author contributions 61 Textual and graphical abstract 62 Introduction 63 Methods 65 2.1 Peptides 65 2.2 Isothermal titration microcalorimetry (ITC) 65 2.3 Preparation of peptide conjugates 65 2.4 Biosorption studies 66 2.5 Model calculation of peptide C3.8 67 Results and Discussion 68 3.1 Interaction studies of free peptides in solution 68 3.2 Biosorption studies with peptide polystyrene conjugates 71 3.2.1 Covalent and site-selective immobilization of peptides 71 3.2.2 Interaction of peptide conjugates with gallium 72 3.2.3 Interaction of peptide conjugates with arsenic 73 3.2.4 Continuous experiments 73 3.3 Model calculation for peptide C3.15 75 Counclusion 76 Acknowledgment 77 CHAPTER V. 78 Obtained insights for the selection of metal-binding peptides in biopanning experiments 79 Conclusions for the development of peptide-based materials for the biosorptive recovery of metal ions from aqueous solutions 81 REFERENCES 85 APPENDIX 94 SUPPORTING INFORMATION FOR CHAPTER IV 94 LIST OF FIGURES 99 LIST OF TABLES 100 LIST OF ABBREVIATIONS 101 LIST OF CHEMICALS 104 ACKNOWLEDGEMENTS 106 CURRICULUM VITAE 109 LIST OF PUBLICATIONS 111

Physical attractiveness plays an important role in many aspects of our lives. The aim of this study is to reveal what is the correlation between rating of physical attractiveness made by oneself and by others, and personal grooming. The relation between self-rating of overall physical attractiveness and its rating made by others (using photographs) and connection between these ratings and personal grooming were examined on the basis of a heterogeneous sample of 43 women and 32 men. These ratings of overall attractiveness, face attractiveness and body attractiveness were provided by 34 judges (each time 17 males and 17 females) different for every variable. Our results showed a positive relation between participant's self-rating of overall physical attractiveness and its rating made by others. Further analyses proved that rating of overall physical attractiveness was positively associated both with facial and body attractiveness for both sexes. With regard to women, a correlation between self-rating of physical attractiveness and reduced care of body shape and eyebrow was revealed; regarding men, self-rating correlated with reduced care of body shape, hair, fingernails and less care for outfit was shown. Concerning female participants, no relation between rating of overall physical attractiveness...

Byggbranschen strävar ständigt efter effektivisering och optimering som kan minska ett projekts byggkostnad samt byggtid. Byggnationen av teknikutrymmen på yttertak upplevs av både konstruktörer och projektledare som ett moment som har en stor byggkostnad och en lång byggtid. Ett teknikutrymme på yttertak har olika förutsättningar i förhållande till den underliggande byggnad, vilket kan leda till att produktionen fördröjs och att byggtiden förlängs. En förutsättning som skiljer ett teknikutrymme från dess underliggande byggnad är att teknikutrymmets ytterväggar kräver ett indrag från fasadlinjen. Detta gör att teknikutrymmets ytterväggar inte alltid placeras i linje med den underliggande vertikala stommen.  De laster som förs ner genom teknikutrymmets ytterväggar skapar punktlaster på teknikutrymmets golvbjälklag. Punktlasterna kan bidra till att golvbjälklaget kräver förstärkningar, vilket är både kostnads- och tidskrävande. En undersökning om stommaterialets påverkan på materialkostnaden och byggtiden för ett teknikutrymme har utförts. I undersökningen analyserades tre olika materialalternativ utifrån förutsättningar och krav som anges i ett specifikt referensprojekt. De material som har analyserats som alternativ för teknikutrymmets stomme är stål, lättelement av trä och betong. Syftet med undersökningen är att analysera vilket stommaterial som är optimalt att använda för teknikutrymmet, med hänsyn till kostnad, tid och bärighet. Resultatet visar att lättelement av trä har den minsta materialkostnaden och att betong har den kortaste byggtiden. Slutsatsen är att det optimala stommaterialet för referensprojektets teknikutrymme är betong. / The construction industry is constantly striving for efficiency and optimization that can reduce a project’s construction cost and construction time. The construction of plant rooms built on roofs is perceived by both structural engineers and project managers as a step in the production that has a big construction cost and a long construction time. A plant room on a roof has other conditions compared to the rest of the building, which can slow down production and the construction time is therefore extended. One condition that separates the plant room from the building is that the plant room’s outer walls require an offset from the facade of the building. This leads to the plantroom’s outer walls not necessarily lining up with the underlying vertical structure. The loads that go down through the plant room’s outer walls create point loads on the plant room’s slab. The point loads could make the slab need strengthening, which is both costly and time-consuming.   An investigation into the material of the structural frame's impact on the construction cost and construction time has been carried out. The aim of the investigation is to analyze which structural material is optimal with regards to cost, time and structural capacity. In the investigation three materials were analyzed by the conditions and requirements from a reference project. The chosen materials were a steel frame as well as light elements made from timber and concrete.   The result shows that lightweight elements made from timber are the most economic option and concrete is the most time-efficient option. The conclusion is that the optimal structural material for the reference project is concrete.

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