Les entérocoques sont des bactéries commensales de l'Homme majoritairement rencontrées dans le tractus digestif. En dépit du caractère bénéfique pour leur hôte, ces microorganismes sont retrouvés au deuxième rang des bactéries responsables d'infection nosocomiales en France ces dernières décennies. Diverses études tendent à montrer que le métabolisme énergétique constitue un facteur crucial pour le processus infectieux des microorganismes. Lors de ce travail, nous nous sommes intéressés à l'étude des métabolismes de différents polymères de glucoses chez Enterococcus faecalis : les maltodextrines et le gentiobiose. L'utilisation du maltose et des maltodextrines est, chez cette bactérie, directement coordonnée au niveau transcriptionnel par le répresseur MalR. L'activité de ce régulateur est rapidement modulée par le maltose qui représente l'inducteur du système et par un corépresseur protéique : la protéine P Ser HPr qui, à l'inverse, favorise la répression exercée par MalR. Le métabolisme des maltodextrines complexes, mais pas le métabolisme du maltose, est également réprimé par le régulateur pléiotrope CcpA en coordination avec son cofacteur P Ser HPr en présence de glucose. La répression catabolique de l'opéron genBA, impliqué dans le métabolisme du β glycoside gentiobiose, est aussi assurée par ce régulateur CcpA en présence de glucose. Cet opéron genBA est responsable de l'import du gentiobiose par un PTS ainsi que de son catabolisme grâce à une hydrolase. L'expression de cette structure opéronique nécessite la présence de l'activateur transcriptionnel GenR actif en présence de l'inducteur gentiobiose 6' P. / Enterococci are commensal bacteria of Humans predominantly encountered in the digestive tract. Despite their beneficial activity for their host, these microorganisms became the second leading bacterial cause of hospital acquired infections in France for last decades. Some studies showed that the central metabolism is a critical factor for microorganisms infection process. In this study, we worked on the characterisation of metabolisms of the different glucose polymers maltodextrins and gentiobiose in Enterococcus faecalis. The maltose and maltodextrins utilization is coordinated in this bacterium transcriptionally by the MalR repressor. The MalR activity is rapidly modulated by the inducer maltose and by the co repressor P Ser HPr which strengthens the MalR DNA binding. The metabolism of long maltodextrins is also repressed by the pleiotropic regulator CcpA in complex with its essential cofactor P Ser HPr in presence of glucose. The Catabolite repression of the operon genBA, involved in metabolism of the β glycoside gentiobiose, is assumed by CcpA in presence of glucose. This operon genBA allows the gentiobiose uptake with a PTS and its catabolism by a hydrolase. The expression of this latter operon requires both the GenR transcriptional activator and the inducer gentiobiose 6' P.

Au cours des dernières décennies, la technologie des nanoparticules pour la délivrance des vaccins au niveau de muqueuses a reçu un intérêt croissant. L’administration intranasale possède de grands avantages pour la stimulation du système immunitaire, telles que la stimulation d’une immunité protectrice locale et systémique. Cependant des systèmes de délivrance et des adjuvants sont souvent nécessaires pour déclencher efficacement la réponse immunitaire. Nous avons appliqué la technologie des nanoparticules en tant que système de délivrance d'un vaccin universel nasal contre la grippe dans un projet européen FP7 appelé UniVacFlu. Nous avons formulé un antigène adjuvé CTA1-3M2e-DD avec les NPL. Cet antigène est composé de la sous-unité A1 de la toxine du choléra et d’un épitope conservé du virus de la grippe A (M2e), ainsi que du dimère de l’analogue synthétique de la protéine A de Staphylococcus aureus (DD). Les nanoparticules utilisées sont poreuses et constituées de maltodextrines réticulées ayant un coeur lipidique (NPL). L’association de cet antigène avec les NPL est quantitative et la formulation est stable pendant au moins six mois à 4°C. Les NPL permettent également de délivrer d’une manière accrue cet antigène dans les cellules épithéliales des voies respiratoires et les macrophages. Actuellement ces formulations sont évaluées chez la souris par le consortium UniVacFlu.L'un des principaux problèmes des vaccins nasal est la toxicité qui peut être provoquée par le passage nez-cerveau de l'un de ses composants. Le but de ce travail est d'évaluer le potentiel des NPL, en tant que vecteurs pour la délivrance des vaccins nasal. Ainsi, nous avons étudié le chargement d’un antigène dans les NPL et sa délivrance dans les cellules épithéliales des voies respiratoires. Notre étude révèle que les NPL interagissent fortement avec les muqueuses et délivrent d’une manière accrue les antigènes dans les cellules. Nous avons également montré l'absence de transcytose et de passage paracellulaire des NPL ou des antigènes délivrés dans un modèle de barrière épithéliale in vitro. Les résultats in vivo confirment l'absence de passage nez-cerveau des NPL et montrent qu’elles prolongent fortement le temps de résidence nasale des antigènes qui sont ensuite éliminés par le tractus gastro-intestinal.Ces résultats mettent en évidence l'intérêt des NPL comme vecteurs pour la prochaine génération de médicaments et de vaccins. / Nanoparticles technology for mucosal delivery of vaccines received a growing interest in the last decades. Intranasal administration owns great advantages for immune system stimulation, such as local and systemic protection against infectious diseases. However delivery systems and adjuvants are often required to efficiently trigger mucosal and systemic immune responses. In this thesis, nanoparticles (NP) have been evaluated as delivery system for a nasal universal influenza vaccine in a People Program of the European Union Seventh Framework Program FP7 called UniVacFlu. The aim of the UniVacFlu network is to develop a universal influenza vaccine administered through the mucosal route. We used porous maltodextrin nanoparticles with a lipidic core (NPL). We loaded an adjuvanted antigen named CTA1-3M2e-DD in the NPL. CTA1-3M2e-DD is composed of the A1 subunit of the cholera toxin and a conserved epitope of influenza A virus (M2e), while DD, dimer of the synthetic analogue of the Staphyloccous aureus protein A, targets B cells. Interestingly the antigen loading in NPL was quantitative for the antigen: NPL 1:5 mass ratio and the formulation was stable for at least six months at 4°C. We assessed the successful delivery of the antigen by NPL in airway epithelial cells and macrophages. These formulations are currently evaluated by the UniVacFlu consortium in mice.One of the main issues of intranasal vaccines is the toxicity that can be elicited by the nose-brain passage of one of their components. We investigated the loading of antigens in NPL and their delivery in airway mucosa. We observed a high endocytosis of NPL and an increased protein delivery into the cells. On a transwell model of the airway mucosa we assessed the absence of transcytosis and paracellular passage of the NPL. In vivo results confirmed the lack of nose-brain passage of the NPL, as NPL were found not to cross the mucosa. Interestingly, we observed an increased nasal residence time of the protein targeted by NPL. The particles after having delivered their payload are totally eliminated through the gastrointestinal tract, making these nanoparticles good candidates for mucosal delivery system. These results highlight the interest of NPL as vectors for mucosal delivery of drugs.

L'optimisation des procédés utilisant un substrat bon marché et abondant est considéré comme un facteur affectant le prix de production du 2,3-BD. Les valeurs optimales du pH, du taux d'aération, de la vitesse d'agitation, et de la concentration en substrat (maltodextrine) pour la production de 2,3-BD à partir de maltodextrines par la souche génétiquement modifiée Klebsiella oxytoca KMS005 ont été déterminées par une méthode conventionnellefacteur par facteur ainsi que par l’utilisation de la méthode des surfaces de réponse avec un plan d’expériences de Box-Behnken. Les résultats ont montré que les valeurs optimales de pH, taux d'aération, vitesse d'agitation, et concentration en substrat (maltodextrine) ont été respectivement de 6,0, 0,8 wm, 400tr/min et 150 g/L. Les surfaces de réponses ont permis de montrer que la vitesse d'agitation était le paramètre le plus influent pour la production de 2,3-BD. La mise en oeuvre d’un procédé fed-batch a permis d’obtenir en 78 h une concentration en 2,3-BD de 88,1±0,2 g/L avec un rendement de 0,412±0,001 g/g et une productivité de 1,13±0,01 g/L/h. L’influence des conditions de micro-aération sur la croissance des microorganismes et sur la production de 2,3-BD a été étudiée. En bioréacteurs batch, le transfert d’oxygène a été caractérisé via la mesure kLa en faisant varier le débit d'air et la vitesse d'agitation. La quantité optimale d'oxygène fournie aété évaluée à 9,5 g correspondant à un kLa de 25,2 h-1. Ensuite, un Ensuite, un procédé fed-batch a été étudié avec différentes stratégies de débit d'alimentation en glucose. Les meilleurs résultats ont été obtenus pour un débit d'alimentation constant en glucose de 2 g/h après une phase batch de croissance de 48 heures, et suivie d'une phase batch finale de 40 heures. Il en est résulté une concentration finale de 2,3-BD de 74,7 g/L avec une productivité de 0,64 g/L/h et peu de sous-produits formés (environ 3 g/L d’acide succinique, acétate et éthanol). D’autre part, les résultats issus des expérimentations en bioréacteur de 2 L ont été mis en oeuvre à l’échelle pilote. La production de 2,3-BD de maltodextrine a été réalisée dans des bioréacteurs de 10, 90 et 300 L pour différentes vitessesd’agitation et un taux d'aération fixé à 0,8 vvm.). Une concentration de 2,3- BD de 53,8 g/L et un rendement de 0,40 g/g de sucre consommé en 48 hont été obtenus avec une vitesse d’agitation constante de 295 tr/min en réacteur de 10 L. Pour le réacteur de 90 L, une concentration en 2,3-BD de52,53 g/L et un rendement de 0,43 g/g de sucre consommé ont été atteints en 72 h pour une vitesse d’agitation constante à 130 tr/min. Les meilleuresconditions d’inoculation ont été obtenues pour les précultures en phase exponentielle de croissance (12 h d’incubation) et une DO550 égale à 4 avant transfert dans le bioréacteur de 90 L. Pour le réacteur de 300 L, la concentration en 2,3-BD était de 45,02 g/L pour un rendement de 0,43 g/g de sucre consommé, obtenusaprès 72 h. / An optimization process with a cheap and abundant substrate is considered one of the factors affecting the price of commercial 2,3-Butanediol (2,3-BD) production. The optimized levels of pH, aeration rate, agitation speed, and substrate concentration (maltodextrin)were optimized by a conventional method and Response Surface Methodology (RSM) with Box-Behnken design in which metabolically engineered Klebsiell oxytoca KMS005 utilized maltodextrin to produce 2,3-BD. Results revealed that pH, aeration rate, agitation speed,and maltodextrin concentration at levels of 6.0, 0.8 vvm, 400 rpm, and 150 g/L, respectively, were the optimal conditions. RSM indicated that the agitation speed was the most influential parameter when either agitation and aeration interaction or agitation and substrate concentrationinteraction played important roles for 2,3-BD production. Under interim fedbatch fermentation, 2,3-BD concentration, yield, and productivitywere obtained at 88.1±0.2 g/L, 0.412±0.001 g/g sugar supplied, and 1.13±0.01 g/L/h, respectively, within 78 h. The influence of micro-aerobicconditions on microbial growth and 2,3-BD production was also studied. In batch bioreactors, air flow rate and agitation rate characterized through kLa measurement were tested. The optimal amount of oxygen supply was evaluated at 9.5 g corresponding to a kLa of 25.2 h-1 for cell growth and 2,3-BD production. Then, a fed-batch process was investigated by different glucose feeding rate strategies. Fedbatch with a glucose feeding rate of 2 g/h starting at the end of the growth phase during 48 h, followed by a final batch phase of 40 h was found satisfactory. It resulted in a final 2,3- BD concentration of 74.7 g/L with a productivity of 0.64 g/L/h but few byproducts formed (about 3 g/L including succinate, acetate and ethanol). Validated information in the 2 L bioreactor was further applied in a larger scale production of 2,3-BD with series of bioreactors from 10, 90 and 300 L vessels. Batch experiments were conducted based on various agitation speeds with the fixedaeration rate at 0.8 vvm. As a result, 2,3-BD concentration, and yield were achieved at 53.8 g/L, and 0.40 g/g sugar supplied within 48 h, respectively, under the constant tip speed at 295 rpm using a 10 L vessel. Its concentration of 52.53 g/L and yield of 0.43 g/g sugar consumedwithin 72 h were attained under the condition of the constant tip speed at 130 rpm using a 90 L fermenter. An appropriate seed inoculum condition was found with an optical cell density (OD550) around 4 at the log phase (12 h incubation) prior to transferring of the inoculum into the 90 L fermenter. Under the constant tip speed at 70 rpm, 2,3-BD concentration and yield were obtained at 45.02 g/L and 0.43 g/g sugar consumed in the pilot scale of 300 L bioreactor after 72 h incubation.

Orientador: Denise Vaz de Macedo / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Educação Fisica / Made available in DSpace on 2018-08-14T02:22:19Z (GMT). No. of bitstreams: 1 Machado_EduardaFariaAbrahao_M.pdf: 1361714 bytes, checksum: 992f3e29c70608cd5aeadd76efd36059 (MD5) Previous issue date: 2009 / Resumo: O exercício físico pode induzir traumas na musculatura, sinalizando uma resposta inflamatória. O reparo e regeneração das estruturas danificadas dependem do período adequado de descanso para recuperação. Esse período regenerativo é necessário após uma única sessão ou diversas sessões de exercícios. A restauração do glicogênio muscular pós-treino tem sido proposto como um dos fatores mais importantes para a recuperação pós-esforço. Normalmente, no esporte, são utilizados monossacarídeos ou amidos em diluições apropriadas para repor o glicogênio pós-treino. O caldo-de-cana é uma bebida comum no Brasil. Possui 65%-75% de água em sua composição média e alta concentração de sacarose, correspondente a 70%-91% de seus sólidos solúveis, além de antioxidantes, vitaminas, minerais e aminoácidos. O objetivo desse trabalho foi analisar o efeito do caldo-de-cana comparado a soluções carboidratadas ministradas imediatamente após um protocolo de exercício exaustivo na reposição de glicogênio; e na cinética de biomarcadores. No entanto, durante a revisão literária encontramos a utilização de diversos tipos anestésicos como principal variação metodológica nos estudos que quantificavam a concentração de glicogênio em animais. Foi necessário, portanto, definir qual anestésico utilizar permitindo interpretação de dados teciduais com análises concomitantes em sangue. Os agentes anestésicos podem afetar estrutura, função de órgãos e sistemas biológicos diferentemente, importando saber se o anestésico a ser utilizado poderia causar hemólise e/ou glicogenólise tecidual, interferentes na interpretação dos resultados. O capítulo 1 apresenta dados da comparação de 3 anestésicos injetáveis em relação ao grau de hemólise e concentrações de glicogênio. Os animais foram divididos em 3 grupos cada qual com um anestésico: Hidrato de Cloral (CH), Ketamina + Xilazina (KX), Zoletil 50® (zolazepam e tiletamina) + Xilazina (ZTX). Os grupos CH e KX exibiram hemólise em graus variados. Já o soro do grupo ZTX não apresentou hemólise. Não houve diferenças significativas nas concentrações de glicogênio entre os grupos CH e ZTX. Já o KX apresentou glicogenólise acentuada em todos tecidos. Os dados apresentados no capítulo 1 mostraram que o anestésico ZTX era o mais apropriado. O Capítulo 2 apresenta dados do efeito do caldo-de-cana comparativamente a soluções carboidratadas ministradas pós-exercício exaustivo, na reposição de glicogênio muscular e hepático, e na cinética de marcadores de proteólise, lesão muscular e inflamação durante 48h de recuperação. Esse estudo foi dividido em 2 experimentos. Os resultados do experimento 1 mostraram que a suplementação com caldo de cana foi tão eficiente quanto à maltodextrina para restaurar o glicogênio muscular. Nenhum dos suplementos foi capaz de repor significativamente o glicogênio hepático. Nos parâmetros bioquímicos e contagem do número de leucócitos totais, analisados no experimento 2, os dados mostraram instalação de quadro inflamatório e dano muscular pós-exercício perdurando pelas 48h de descanso. As amostras dos grupos suplementados com caldo-de-cana e maltodextrina não alteraram o padrão de resposta nas 48h pós-exaustão. Uma provável explicação seria a suplementação aguda, e após uma sessão de exercício, não ter sido suficiente para desencadear alterações nas análises. As potencialidades dos constituintes do caldo-de-cana e a escassez de estudos científicos com objetivo de utilizá-lo como recurso ergogênico no esporte reforçam a continuidade dessas investigações. / Abstract: Physical exercise induces traumas to biological structures which signal inflammatory process activation. The repair and regeneration of the damaged structures depend on an appropriate rest period for the recovery. This regenerative period is necessary after a single session or after several sessions of exercises. The muscle glycogen repletion after physical exercise seems to influence the recovery time. Usually in practices the carbohydrate rich compounds like maltodextrine, fructose or dextrin are normally used to maximize the rate of glycogen storage in the early hour's post-exercise. Sugar cane juice is an appreciate product and easily to find in Brazil. It possesses 65%-75% of water and a high sucrose concentration that corresponds 70%-91% of their soluble solids, phenolics compounds, vitamins, minerals and amino acids. The goal of this master's degree dissertation was to analyze the effect of sugar cane juice comparatively to other carbohydrates solutions supplied immediately after a bout of exhausting exercise in rats in the replacement of the glycogen stores; and in the kinetics of some biomarkers in 48h post-effort. However, the literature revision found the use of several anesthetics as the main methodological variation in the studies that quantified glycogen concentration in animals. It was necessary, therefore, initially to define the anesthetic that could allow the association of the data obtained in tissues with concomitant analyses in blood. It was important to know if the anesthetic used for samples collection could cause haemolysis and/or glycogenolysis in the animals. The studies accomplished during the master's degree are contained in two chapters. In chapter 1 we presented the comparison data of three injectable anesthetics used in experiments with animals, concerning the degree of haemolysis and glycogenolysis after anesthesia. The animals were divided into three groups: Cloral Hydrate (CH), Ketamine + Xylazine (KX), Zoletil 50® (zolazepam and tiletamine) + Xylazine (ZTX). The CH and KX presented serum haemolysis. Only ZTX presents no detectable values. The average value of the hepatic and muscular glycogen concentrations exhibited no significant difference between CH and ZTX. However, the KX presented accentuated glycogenolysis in all tissues. Our data suggest that the anesthetic ZTX seems to be the most appropriate for studies that need simultaneously to quantify the concentration of glycogen and blood markers without interferences. Chapter 2 presented the data of the effect of sugar cane juice comparatively to other carbohydrates solutions supplied after the exhaustion in rats, in the replacement of muscular and hepatic glycogen stores, and in the kinetics of some markers of proteolyses, muscular lesion and inflammation in 48hs of recovery. For that, we divided this study in two experiments. The results presented in the experiment 1 showed that the supplementation with sugar cane was as efficient as maltodextrine to restore the gastrocnemium red and white portions glycogen after 1 h of the exhaustion. The fructose exhibited less pronounced effect. None of the supplements were able to restore the hepatic glycogen significantly. The biochemical parameters and WBC number data analyzed in experiment 2 showed an persistent inflammatory picture associated to muscular damage even after 48h of rest. The supplemented groups with sugar cane and maltodextrine didn't alter the 48h post-exercise response pattern. Their representatives' potentialities reinforce the continuity of the investigations. / Mestrado / Biodinamica do Movimento Humano / Mestre em Educação Física

Orientador: Celio Kenji Miyasaka / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-08T11:44:53Z (GMT). No. of bitstreams: 1 Lollo_PabloChristianoBarboza_M.pdf: 4184736 bytes, checksum: 3b13bac14d821be3f7d31a4ad595e6b2 (MD5) Previous issue date: 2007 / Resumo: O futebol assim como outras modalidades esportivas vem utilizando os conhecimentos científicos produzidos para a preparação dos atletas. Esse melhor preparo resulta em distâncias percorridas durante o jogo cada vez maiores além de um melhor desenvolvimento da musculatura para desempenhar as tarefas necessárias durante as partidas. É reconhecido que essa atividade física eleva as necessidades protéicas dos atletas, porém não se sabe exatamente qual é o requerimento protéico dos atletas de diferentes modalidades esportivas. Os principais motivos citados para esse aumento no requerimento protéico de atletas são: hipertrofia muscular (em determinadas fases do treinamento); oxidação de proteínas corporais durante atividades de longa duração para fornecimento de energia (via esqueleto carbônico dos aminoácidos de cadeia ramificada - BCAA); danos em proteínas musculares decorrentes de alterações fisiológicas causadas pelo exercício (queda de pH, elevação da temperatura intramuscular e tensões mecânicas nos músculos e demais estruturas do aparelho locomotor). As proteínas de soro de leite são consideradas excelente fonte de BCAA, e possuem alto valor biológico. Objetivo: verificar os efeitos da suplementação com proteínas de soro de leite na composição corporal, desempenho físico e parâmetros bioquímicos de futebolistas. Metodologia: quarenta e oito futebolistas juvenis (masculino), idade 16,7 + 0,6 anos, medindo 179,2 cm + 6,7 e pesando 74,42 kg + 6,44, foram realizados 2 experimentos (n=24 em cada experimento), o primeiro experimento submeteu os 24 atletas a suplementação protéica diária de 1g.kg-1.dia-1 mais 0.4 g.kg-1.dia-1 de carboidrato (sacarose) por 8 semanas com as diferentes proteínas: a) caseína (CAS, n=8); b) proteína do soro de leite isolada (PSLI1, n=8); c) proteína do soro de leite hidrolisada (PSLH2 n=8). O segundo experimento submeteu 24 atletas à suplementação protéica ou glicídica diária de 1g.kg-1.dia-1 por 12 semanas com: a) maltodextrina (MALTO, n=8); b) PSLI2 (n=8); c) PSLH2 (n=8). Foram realizados testes antropométricos (composição corporal), de desempenho físico ¿ ¿Yo-yo intermittent recovery level 2¿, saltos verticais, 4 minutos contra o relógio, 3000m e 3200m à 85% da freqüência cardíaca (FC) máxima e os parâmetros bioquímicos: ácido úrico, colesterol, HDL, creatinina, glicose plasmática e dosagem das enzimas: creatina quinase (CK) e lactato desidrogenase (LDH). Resultados: após a suplementação, os grupos CAS e PSLI2 aumentaram significativamente a massa muscular em 2,83% e 3,36% respectivamente. No desempenho físico, foi observado que os atletas dos grupos PSLI2 e PSLH2 aumentaram a distância percorrida em 4,44% e 3,41% respectivamente no teste 4 minutos contra o relógio. No teste de 3200m em 85% da FC máxima o tempo dos atletas dos grupos PSLI2 e PSLH aumentaram o tempo em 5,48% e 6,8%. Nos parâmetros bioquímicos analisados verificamos queda significativas nas enzimas indicadoras de lesão muscular nos atletas dos grupos PSLH2 e aumento significativo no ácido úrico nos atletas dos grupos, PSLI1, MALTO, PSLI2 e PSLH2 / Abstract: The soccer as well as other sporting modalities comes using the knowledge produced for training the athletes. These better training results in large distances covered during the game. So the better preparation of the muscle is need for the soccer players. The physical activity raises the proteins requirements, however, how much is not known accurately. The main reasons cited for this increase in the proteins requirements in athlete are: muscular hypertrophy (in determined phases of the training); body protein oxidation during exercise of long term for energy supply (main skeleton carbonic of the branched chain amino acids - BCAA); damages in muscular proteins decurrently of physiological alterations caused by the exercise (fall of pH and rise of the temperature intramuscular and mechanical tensions in the muscles and another structures of the locomotive device). The whey protein is excellent source of (BCAA), and protein of high biological value. Objective: to verify the effect of the supplementation with whey proteins in the body composition, physical performance and biochemistry parameters of soccer players. Methodology: Forty eight youthful soccer players (masculine), age 16.7 + 0.6 years, heighted 179.2 cm + 6.7 and weighted 74.42 kg + 6.44, in 2 groups (n=24 in each group), the first group was submitted to the daily protein supplementation of 1g.kg-1.dia-1 plus 0.4g.kg-1.dia-1 of carbohydrate (sucrose) for 8 weeks with different proteins: a) the casein (CAS, n=8); b) whey protein isolated (PSLI1, n=8); c) whey protein hydrolyzed (PSLH2 n=8). The group 2 was submitted to daily the protein supplementation of 1g.kg-1.dia-1 for 12 weeks with: a) maltodextrine (MALTO, n=8); b) PSLI2 (n=8); c) PSLH2 (n=8). Anthropometric tests had been carried through (body composition), of physical performance ¿ Yo-yo intermittent recovery level 2, jump tests, 4 minutes against the clock, 3000m and 3200m to 85% of the cardiac frequency (FC) maximum ¿ and biochemistry tests - acid uric, cholesterol, HDL, creatinine, plasmatic glucose and enzymes: lactate dehydrogenize (LDH) and creatina kinase (CK) and anthropometry. Results: After the supplementation groups CAS and PSLI2 had increased significantly the muscular mass in 2,83% and 3,36% respectively. In the physical performance, it was observed that groups PSLI2 and PSLH2 had increased in the distance covered in 4,44% and 3,41% for PSLI2 respectively in test 4 minutes against the clock. In the test of 3200m to 85% of the maximum FC the time of the groups PSLI2 and PSLH had increased the time in 5,48% and 6,8%. In the biochemistry parameters it was verified significantly reduction in enzymes of muscular injury in group PSLH2 and significant increase in the uric acid of groups PSLI1, MALTO, PSLI2 and PSLH2 / Mestrado / Nutrição Experimental e Aplicada à Tecnologia de Alimentos / Mestre em Alimentos e Nutrição