Fixed nitrogen is important as a nutrient for organic matter formation and as an electron donor (nitrification) and acceptor (denitrification) for energy generation, but it is scarcely available in aquatic systems. Nitrification oxidizes ammonium to nitrite and nitrate. Denitrification uses these fixed species to form dinitrogen gas. The classic understanding of the nitrogen cycle requires dissolved oxygen for nitrification and assumes denitrification reduces nitrate to dinitrogen through various intermediates in anaerobic conditions. The global nitrogen budget is imbalanced with more marine denitrification measrued than previously estimated in the classic nitrogen cycle, suggesting alternative anaerobic nitrification and denitrification pathways exist. One alternative denitrification pathway is anammox, which directly oxidizes ammonium to dinitrogen with nitrite as the electron acceptor. Other alternative pathways for both nitrification and denitrification involve redox metals as catalysts. Manganese-catalyzed anaerobic nitrification and denitrification are thermodynamically favorable at neutral pH. However, experimental evidence for these processes is still lacking. This investigation seeks to uncover evidence of manganese-catalyzed nitrification and denitrification in saltmarsh sediments. Batch reactors with anaerobic sediment slurries from a saltmarsh in coastal Georgia were incubated in the presence and absence of colloidal manganese oxides and isotope-labeled ammonium and nitrate to trace dinitrogen formation. Results show that denitrification is more prominent in the manganese-treated reactors and that the classic denitrification pathway may not be substantial in shallow saltmarsh sediments. These data indicate that anammox and/or manganese-coupled denitrification are major contributors to the removal of fixed nitrogen. Ammonium removal in the manganese-treated reactors is accompanied by a high nitrite production compared to the nitrogen-only treatment, indicating manganese-coupled denitrification exists and/or anammox is promoted in the presence of manganese. Primary productivity is generally high in saltmarshes, but oxygen penetrates less than a few millimeters in the sediment. These observations suggest that oxygenic nitrification does not fuel denitrification below the sediment-water interface. The data show that manganese may play a role in the formation of nitrite and nitrate in oxygen-limited sediments.

Eutrophication, particularly due to nitrogen (N) and phosphorus (P) input, has been massively altered by anthropogenic activities. Thus it is important to understand the impact on salt marsh plants; however studies on salt marsh plants within Galveston Bay, Texas are limited. In this study, the effects of repeated nutrient additions in monospecific plots of Spartina alterniflora, Batis maritima¸ and Salicornia virginica as well as mixed plots of B. maritima and S. virginica were studied over 15 months. Results showed that nutrient loading led to an increase in height, biomass, growth rate and percent nitrogen (N) within all three species studied, but were species specific more than dose dependent. Nitrogen content in leaves had a positive correlation with P content in leaves but a negative correlation with carbon (C) content. Nutrient loading lead to a significant increase in total chlorophyll in the fertilized plots of S. alterniflora and S. virginica one month into the study. Nutrient addition to two succulent species, B. maritima and S. virginica in mixed plots did not reveal a distinct superior competitor within the 15 month study in terms of growth and nutrient use efficiencies; however using the maximum growth rates of the monospecific plots, the Monod model was used to determine which species would dominate at high nutrient loads. Based on height data S. alterniflora would dominate, while B. maritima would dominate according to the Monod model based on biomass.

タンデトロン加速器質量分析計業績報告 Summaries of Researches Using AMS 2002 (平成14)年度

Understanding the salt marsh ecosystem in the Guadalupe Estuary is needed because wetlands in this system support the endangered whooping crane (Grus americana). The marsh plant research and monitoring described herein were based in the salt marshes at Aransas National Wildlife Refuge (ANWR), which are utilized by the cranes each winter. Past research indicates that the Carolina wolfberry (Lycium carolinianum) contributes 21-52% of crane energy intake early in the wintering period (Chavez 1996). Beginning in Fall 2003, vegetation transects were sampled along an estuarine gradient at ANWR. Species diversity and composition was similar at the three sites, with all sites containing the same 6-7 common species. While Spartina alterniflora is only a minor part of this vegetation community, it dominates the few low inter-tidal, fringe areas present. Species composition exhibited little variability from Year 1 to Year 2 of the study. Densities and biomass of L. carolinianum were not significantly different between sites or years. L. carolinianum, while important to salt marsh ecology, accounts for only a small portion of the overall productivity. Based on correlation coefficients, L. carolinianum was found in association with some of the common species in the vegetation community, indicating that its growth and survival requirements are typical to the salt marshes at ANWR. Also beginning in Fall 2003, I repeatedly sampled L. carolinianum in permanent plots along the estuarine gradient. L. carolinianum exhibits strong temporal patterns. Leaf production peaked in early spring and again just prior to peak berry abundance. Flowering of L. carolinianum occurred in October and November. Peak berry abundance coincided with the cranes’ arrival in late October and early November. Berry production occurred in October, November, and December; berries were virtually non-existent in the marshes for the remainder of the year. Stepwise regression showed stem diameter alone was a good estimator of aboveground biomass of this species in ANWR marshes, accounting for 94% of the variability (p<0.001). Changes in aboveground biomass followed no distinct patterns in the year of monitoring, perhaps due to the woody stem of the plant. Spatial patterns in L. carolinianum were not explained by water quality parameters alone; it is suggested that soil properties may help to account for the spatial variability.

The purpose of the study is to identify and quantify the hydrologic and ecologic differences between two adjacent sections of Colt Creek; one section unaltered and one section altered by clearing and drainage. These differences were measured by monitoring water levels, groundcover vegetation in each of the two areas, and monitoring numbers and species of birds utilizing the two areas. Surface water levels were measured in three locations: in the historic Colt Creek flow way, in the ditch draining the creek, and in an adjacent wetland strand. In addition, a shallow monitor well in the creek was used to measure groundwater levels when the creek was dry. The intent of avian monitoring was to use birds as a relatively easily observable surrogate for wildlife habitat utilization in general. Groundcover vegetation species and approximate percent cover data were recorded at several locations in both wetlands. Data collection occurred from January 2010 to January 2011. The results indicate that the hydrology, vegetation, and avian utilization of the two adjacent areas were substantially different. Specifically, the hydroperiod during the monitoring period was seven weeks shorter in duration in the downstream area than in the upstream unaltered area. In addition, the presence of flowing water, i.e., stream flow, through the downstream area was approximately 18 weeks less than the upstream area. Vegetation species composition, diversity, and percent cover also differed in the two areas. A total of 39 groundcover species were identified in the two sites. Seven (7) additional plants were identified to genus. Twenty one species (74.9 %) of all plants identified were common to both areas. Sixteen species (41.0 %) were found only in the unaltered site and 10 species (25.6 %) were found only in the altered site. Species richness was greater in the unaltered site while percent cover was less, i.e., more bare ground / plant litter. Relative percent cover by wetland species in the unaltered site was 11.8 percent greater than in the altered site. Finally, avian utilization was greater in the altered area, as 484 individual birds and 27 species were identified in the altered site compared to 138 individual birds and 13 different species identified in the unaltered area.

I estimated patterns of shorebird abundance and species diversity in the Colorado River Delta and Upper Gulf of California wetlands in order to determine the relative contribution of intertidal wetlands and non-tidal anthropogenic wetlands to support shorebird habitat use. Species richness varied from 15 to 26 species among sites and 29 species were detected across sites. Density during the peak migration month was higher at the anthropogenic wetland Cienega de Santa Clara (mean = 168 ind/ha, 95% C.I. 29-367), and the intertidal Golfo de Santa Clara (mean = 153 ind/ha, 95% C.I. 17-323). Anthropogenic wetlands (playa and lagoons) supported high abundance of shorebirds along with intertidal wetlands in the Colorado River Delta (mudflats). In contrast, intertidal wetlands farther south on the Sonoran Coast presented lower abundance but higher diversity of shorebird, likely as a result of the higher diversity of habitats (rocky shore, sandy beach, estuary). I modeled water management scenarios for the Cienega in order to determine the response of the dominant vegetation (southern cattail, Typha domingensis Pers.) and the area of the outflow pool below the marsh to different scenarios of water management. The model indicates that if the inflow rate is reduced below the current 4-5 m³s⁻¹ the vegetated area of the Cienega that supports habitat for marsh birds would decrease in proportion, as would the area of the outflow pool in the Santa Clara Slough identified previously as shorebird habitat. Increases in salinity will also reduce the vegetated area due to the low salt tolerance of T. domingensis. In winter about 90% of inflow water exits the Cienega into the Santa Clara Slough due to low evapotranspiration contributing to inundate areas that are used by wintering and migrating shorebirds. Lastly, I explored the feasibility of using Vegetation Indices (NDVI and EVI) to model Yuma Clapper Rail detections in the Cienega de Santa Clara as well as the effects of adding other habitat variables and the presence of fire events in the performance of linear models based on NDVI. Both NDVI and EVI were positively related to the Yuma Clapper Rail detections. The relationship was weak to moderate, but significant (P<0.001), which suggests other factors besides the vegetation condition play an important role in the bird distribution pattern. A model including all the variability among years was a better predictor of the rails detected per transect, than models for fire and non-fire years. We did not find a significant effect from adding habitat features (water % or vegetation %), and we recommend to include variables at both microhabitat level and landscape level, relevant before and during the breeding season in order to increase the explanatory power of models.

Daugelis kaimo turizmo, rekreacinių teritorijų kūrėjų pateikę specialiai sudarytai komisijai parengtus projektus galėjo gauti nemenką ES paramą, tačiau pasisekė ne visiems, kiti rekreacines teritorijas turėjo rengti už savo lėšas. Vienas tokių projektų yra rekreacinės teritorijos įrengimas Pajaurės pelkėje, esančios 20 km nuo Vilniaus centro. Projektas įdomus tuo, kad rekreacinę teritoriją planuojama įrengti pelkėje, kuri pasižymi silpnu gruntu - durpe, o tai reiškia, kad pelkinį paviršių teks pakeisti į patrauklesnį rekreacijai paviršių. Pagrindiniai teritorijos kūrimo darbai bus žemės darbai, kuomet teks daug grunto iškasti formuojant ežeriukus ir protakas, po to užvežti mineralinį gruntą (smėlį), kuris yra labiau tinkamas rekreacijai. / Lithuania is in surplas damp zone, 56% of its territory is waterlogged lands and marshes. Marshes are very important, because them maintain water balance, reduce the flood influence, catching the water, sediment materials and polluted materials. Aim of the work – to do geological wrings and measure up peat and sandy lands layer ply and calculate peat pressure. To achieve this aim, the following tasks were solved: 1. To traverse Pajaurė marsh condition nowadays; 2. To take a peat and measurement density and damp; 3. Find out sandy land layer ply; 4. To traverse Notiškis and Ežeraitis lakes clean-up technology. The research on the use recreation areas of hotel “Le Meridien Villon” for recreation and rest has been accomplished. The recreation zone environment legal base has been evaluated. Hydrological, hydrogeological, peat and sapropel quality reseach of Pajaurė marsh, Notiškis, Varlinis and Ežeraitis lakes has been evaluated, Notiškis Varlinis and Ežeraitis lakes as well as Pajaurė marsh reform and their use for the recreation possibilities have been evaluated as well. The Notiškis and Ežeraitis lakes partial dredging, deeping, arrangement inshore the banks of lakes have been planned. In Pajaurė marsh, 10 new small lakes (8,5 ha of the total area) have been projected by digging the marsh ground and moulding 2,3 m water depth in them, raising marsh surface for boating. Without the undamaged recreation area water balance system, invariable water inflow basin, control water... [to full text]

Unclear predictions surrounding climate change, associated sea-level rise and potential impacts upon coastal environments have placed an emphasis on the importance of sea-level change. Past sea-level fluctuations have been measured using biological and geomorphological forms of evidence. One such biological proxy is salt-marsh foraminifera, which have been used as a high-resolution indicator of past sea-level change, based on the assumption that surface foraminiferal assemblages are similar in composition to buried fossil foraminifera. In South Africa, there is ongoing research seeking to produce high-resolution records of sealevel change, however foraminifera remain an underutilized source of proxy evidence. This research applies salt-marsh foraminifera as precise indicators of relative sea-level change at Kariega Estuary on the Eastern Cape coastline of South Africa. Distributions of modern foraminiferal assemblages were investigated, revealing vertical zonation across the intertidal zone. The foraminiferal and marsh vegetation zones were in part similar and overlapped to a certain extent, identifying three zones; high, low and tidal flats. This suggested foraminiferal distribution is a direct function of elevation relative to tidal fluctuation. A 94 cm core consisting of peat, sand and clay sediments was extracted from the salt marsh. A chronological framework for the core was based on five AMS radiocarbon age determinations of both bulk sediment and shell fragment samples placing the record within the last 1500 years Before Present (BP). The basal shell age was a clear outlier to all bulk sediment ages, possibly as a result of shell recrystallisation. The bulk sediment age determinations suggested two possible age reversals, potentially linked to sedimentary hiatus or contamination. These inconsistencies in the chronology were best viewed as separate age models. The core was analysed at a high resolution, whereby fossil foraminifera were extracted every 2 cm’s down the core. A transfer function was applied to calculate the former elevation at which each core sample once existed, to produce a relative sea-level reconstruction. The reconstruction was related to the age models to produce two possible sea-level curve scenarios. Reconstructed curves from both scenarios depict a 0.5 m (±0.16 m) sealevel highstand at 1500 cal years BP followed by a lowstand of -0.6 m (±0.03 m). Scenario One reached its lowest recorded sea-level between 600 cal years BP and 500 cal years BP and then fluctuated below present day levels. Scenario Two reached its lowest recorded sea-level around 1200 cal years BP, followed by low amplitude fluctuations and a relatively stable period from 100 cal years BP till the present day. The 1500 cal years BP highstand recorded for both scenarios correlates well with existing palaeoenvironmental literature from the southern African coastline. Chronological limitations associated with the remainder of the record hinder inter-comparison with previous studies. The outcomes of this research suggest that intertidal saltmarsh foraminifera demonstrate enormous potential for the high-resolution reconstruction of relative sealevel change in the South African context. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2013.

Little is known about the distribution and habitat suitability of yellow rails (Coturnicops noveboracensis) throughout their breeding range. Yellow rail and vegetation surveys were conducted at 80 wetlands in south-central Manitoba in 2010-2011 to evaluate the effectiveness of repeat-visit, call-broadcast night surveys for detecting this species and habitat associations of this species at the 3-km landscape, patch, and plot scales. Yellow rails were detected at 44% of the study wetlands. Yellow rail detection was imperfect (0.63 in each year), but call-broadcast increased the number of yellow rails detected. Future yellow rail survey efforts should employ call-broadcast and at least three surveys per survey point. Yellow rail presence was positively influenced by the amount of marsh/fen in the landscape and the proportion of rushes at the study wetlands. These characteristics should be considered when identifying potential yellow rail habitat in south-central Manitoba.

The Lower Keys marsh rabbit (Sylvilagus palustris hefneri, LKMR), 1 of 3 subspecies of Sylvilagus palustris, is endemic to the Lower Florida Keys. The LKMR is listed as an endangered species due to predation by feral and free roaming domestic cats (Felis catus) and raccoons (Procyon lotor), road mortality, effects of storm surges, sea level rise, the small declining metapopulation size, and possible habitat loss from hardwood encroachment. The purpose of this study was to determine the current LKMR density on lands managed by the United States Navy, Naval Air Station Key West and evaluate how vegetation structure and patch elevation effect LKMR population density. I conducted fecal pellet counts to determine LKMR density, collected vegetation data using percent composition of ground cover, Robel range pole, and point-centered quarter methods, and obtained data on patch area and elevation. I used simple linear regression to assess the relationship between LKMR density and 9 measured vegetation characteristics, patch area, and patch elevation to determine which variables have an influence on LKMR density and the relationship between them. In my examination of the simple regression models, 6 out of the 11 variables appeared to influence LKMR population density. The average per patch percent composition of nonliving material and grasses, maximum height of vegetation at the range pole, distance to nearest woody vegetation, patch elevation, and visual obstruction readings (VOR) individually accounted for 26.4%, 30.4% , 18.1%, 8.5%, 6.8%, and 1.4% of the variability in LKMR density, respectively. According to the regression models, LKMR density increased in patches with greater amounts of grasses and with greater distance to woody vegetation. Habitat management is vital to the recovery of the LKMR and needs to focus on providing greater amounts of grasses and reducing the amount of woody vegetation encroachment to enhance LKMR population density.