International Telemetering Conference Proceedings / October 27-30, 1997 / Riviera Hotel and Convention Center, Las Vegas, Nevada / Alliant Techsystems’ Advanced Technology Applications organization incorporates the latest IRIG standards for range equipment and operation. Over the past five years, our objective has been to assure interoperability among diverse data recording users while achieving technical excellence for our ADARIO(Analog Digital Adaptable Input Output) family of products. In this paper, we summarize 25 years of ADARIO development; technical challenges, risks and processes; as well as our five-year effort to modify and develop our recording system products to meet the evolutionary standards of technical excellence.

Contemporary proximity extension assays (PEAs) are used for qualitative proteinquantifications in serological samples, with possibilities for scaling assays in multiplex. Medical research can however benefit from robust immunoassays functional for assessingprotein levels in other types of biospecimens. Formalin-fixed paraffin embedded (FFPE)tissues have long been used for morphological studies. The proteome encapsulated byextensive cross-linking from formalin fixation has however impeded the development ofproteomic analysis from the vast biorepositories FFPE-tissues constitute. In this study, Ipresent a proof of concept for assessing FFPE-samples in multiplex format through PEA.Furthermore, a homogenization and protein extraction protocol for assessing fresh-frozentissue with PEA is presented, together with a novel sample buffer for which remarkable risesin protein detection can be seen in several protein assays. Together, these findings extend theapplication area of PEA to tissues together with improved quantification characteristics.

This report is the result of a project in the course Independent Projekt in Molecular Biotechnology at Uppsala University during the spring of 2014. The foremost purpose of the course is to give students the opportunity to carry through exstensive work in a project environment. This project was formed based on a comission from the biotechnology company SweTree Technologies, and the goal has been to compose a summary of the different techniques and methods that exist in the field of mass propagation of trees through the method of somatic embryogenesis. The project group has obtained information about the area mainly throgh reading patents, trying to find key components and bottlenecks in other companies’ somatic embryogenesis technologies. This paper is divided into different sections, containing the patents of the automation of different steps in the process. This is to make it easier for readers to find information about the area they are interested in, as well as to illustrate the main parts of the process as percieved by the project group. Currently, there are several automated solutions for almost every step in the process, some of which are already in use. All the information obtained shows that the cost and labour has decreased with the development of this technology. While there is still room for significant devolopment in order to produce a complete automated process, there is no doubt that this method is becoming an ever more important asset in the area of forestry. Our hope is that this report may be a useful tool for companies or laymen to geta grasp of the field of automated mass production of trees.

Orientador: Eduardo Daruge Junior / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-03T09:15:14Z (GMT). No. of bitstreams: 1 Almeida_IsaAzevedode_M.pdf: 4484053 bytes, checksum: e5137b49df6ad4e6c1204bd7f37d39a9 (MD5) Previous issue date: 2003 / Resumo: Nas últimas décadas, as Ciências Forenses vêm evoluindo de maneira a esclarecer e auxiliar cada vez mais precisamente a justiça. O presente trabalho, através da utilização de 25 amostras de sangue de cadáveres do Instituto Médico Legal de Cuiabá-MT, teve como objetivos: estabelecer a forma de coleta do material biológico através da utilização de cartões FTA@; aprimorar a extração do DNA em amostras de sangue, utilizando a Resina Quelante CHELEX@; aprimorar a PCR utilizando os primers para os loei DYS 3851/11, DYS 3891/11, DYS 390, DYS 391, DYS 392, DYS 393, DYS 19 através da padronização do Nanoplex; comprovar a importância do estudo do cromossomo Y nos casos de investigação de paternidade e em casos forenses. Com o desenvolvimento do trabalho, pôde se observar que os métodos de coleta das amostras de sangue e extração do DNA foram satisfatórios, pois obteve-se uma quantidade de DNA suficiente para o seu estudo. O estudo do Nanoplex fez-se com sucesso, pois, por meio de sua análise em um seqüenciador automático (ASI PRISM DNA Sequencer 377), pôde se detectar os fragmentos, estipulando-se, assim, seus respectivos alelos / Abstract: In the last decades, the Forensic Sciences come evolving in way to clarify and to assist the justice. The present work through the use of 25 samples of corpse blood of the Legal Medical Institute of Cuiabá-MT had as objective: to establish the fonn of collection of the biological material through the use of cards FT A@; to improve the extraction of the DNA in samples of blood being used the Resin Quelante CHELEX@; to improve the PCR using primers for locus DYS 385 1/11, DYS 389 1/11, DYS 390, DYS 391, DYS 392, DYS 393, DYS 19 through the standardization of the Nanoplex; to prove the importance of Y chromosome study in the cases of patemity and in forensic cases. Wlth the development of the work it could be observed that the methods of collection of blood samples and extration of the DNA had been satisfactory, therefore got a enough amount of DNA for its study. The study of the Nanoplex one became successfully, therefore through its analysis in an automatic sequencer (ASI PRISM DNA Sequencer 377), it could be detected the fragments, stipulating its respective alleles / Mestrado / Odontologia Legal e Deontologia / Mestre em Odontologia Legal e Deontologia

Puesta a punto PCR Multiplex para el diagnóstico de 14 virus respiratorios

Orientador: Rege Romeu Scarabucci / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia / Made available in DSpace on 2018-07-19T03:56:13Z (GMT). No. of bitstreams: 1 Kin_KweiYin_M.pdf: 2789721 bytes, checksum: 0126a4b3eb779ac4ab37118882e533da (MD5) Previous issue date: 1974 / Resumo: No presente trabalho são projetados um codificador A/D e um decodificador D/A não lineares para sistema MCP de 30 canais telefonicos, de acordo com as recomendações estabelecidas pela C.C.I.T.T. Este sistema CODEC faz parte de um projeto maior, um sistema de comunicação por meio de modulação de códigos pulsados, ora em desenvolvimento no Departamento de Eletrônica e Comunicações da Faculdade de Engenharia de Campinas. O CODEC não linear projetado e um CODEC de 8 bits que usa compressão de caracteristica logaritmica, dada pela "Lei A=87,6", aproximada por 13 segmentos lineares. / Abstract: This paper presents the system development of a non-linear A/O and D/A converters for a PCM system of 30 voice channels, based on the recommendations established by C.C.I.T.T. This CODEC system is a part of a larger project, a, communication system utilizing Pulse code modulation methodt in progress at the Department of Electronics and Communication of the School of Engineering of Campinas. The proposed non-linear CODEC is an 8 bits CODEC that utilizes a compression with logarithmic characteristic, given by "Law A=87,6", approximated ed by 13 linear segments. / Mestrado / Mestre em Engenharia Elétrica

archives@tulane.edu / Placental insufficiency is a term used to describe the reduced transport of gases and nutrients across the placenta. As a result of placental insufficiency, preeclampsia and intrauterine growth restriction (IUGR) can develop. Preeclampsia is diagnosed by the onset of high blood pressure and proteinuria after 20 weeks of gestation and complicates 2-8% of all pregnancies. Having preeclampsia is also a risk-factor for developing IUGR, which is defined as an estimated fetal weight below the 10th percentile. These conditions can negatively impact the immediate outcomes of pregnancy by reducing placental perfusion and causing placental ischemia. Currently, there is a need for reliable, inexpensive tools that can monitor placental function bedside. Spectral photoacoustic (PA) imaging presents a solution to this need. Photoacoustic imaging uses nanosecond light pulses to excite photoabsorbers within tissue. These photoabsorbers undergo thermal expansion and relaxation, emitting a pressure wave that can then be read by an ultrasound transducer. In this study, the photoabsorbers of interest include hemoglobin, deoxyhemoglobin, and indocyanine green (ICG). Various ultrasound contrast agents are available for clinical use; however, indocyanine green-loaded nanodroplets present various advantages over other contrast agents due to their smaller size and longer in vivo stability. Upon laser irradiation, these droplets experience a liquid-to-gas phase change and provide improved photoacoustic contrast. These nanodroplets are a focus of this work, as they have the ability to be targeted to specific tissues and can act as oxygen carriers. In this case, they are targeted to folate receptor α, which is highly expressed on the placenta. Thus, the first aim of this work is to validate the folate receptor α targeting mechanism using an in vitro tissue phantom model. As oxygen carriers, they are able to release oxygen once activated by the laser. The second aim is to validate localized oxygen delivery via these nanodroplets by using multiplex imaging to determine ICG accumulation and measure their effect on placental oxygen saturation in vivo. Another contrast agent, 2-Deoxy-D-Glucose (2DG-ICG), could be utilized in conjunction with photoacoustic imaging as a tool to monitor glucose transport, a major indicator of placental function. By conjugating ICG to 2DG, a glucose analog, it is possible to target glucose transporter 1, which is the primary glucose transporter on the placenta. The final aim of this work is to determine the feasibility of using 2DG-ICG as an indicator of glucose transport in the placenta using an in vitro model. / 1 / Sarah Nwia

Made available in DSpace on 2014-06-11T19:30:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2005-11-16Bitstream added on 2014-06-13T18:40:37Z : No. of bitstreams: 1 bertholo_lc_dr_arafcf.pdf: 784726 bytes, checksum: 896d3466d9d683acd711a4c3515e98a6 (MD5) / Universidade Estadual Paulista (UNESP) / As hemoglobinopatias constituem um grupo de alterações hereditárias prevalentes em muitas regiões do mundo, atingindo a população brasileira de forma significativa; sendo decorrentes de alterações em genes estruturais, responsáveis pelo aparecimento das hemoglobinas variantes ou em genes reguladores, resultando as talassemias. Sendo assim, foram propósitos do presente trabalho estabelecer metodologia laboratorial embasada em estudo molecular que possibilite o auxílio diagnóstico de hemoglobinas anormais observadas na população brasileira e sem caracterização completa ou pouco informativa; utilizar primers que se acoplem exatamente na posição da mutação do alelo mutante e na respectiva posição do alelo normal, com possibilidade da realização de amplificação gênica alelo específica e com esses conhecimentos, estabelecer protocolos de aplicação laboratorial para uso na rotina. As amostras de estudo foram constituídas por 20 modelos de mutações pertencentes a portadores das mesmas e que inicialmente apresentavam alterações em seu perfil eletroforético, sendo coletadas ou do próprio sujeito da pesquisa ou obtidas de banco de amostras. Os indivíduos eram de ambos os sexos, com diferentes idades e características raciais (caucasóides e não caucasóides) e pertencentes às diversas classes econômicas. Os resultados obtidos permitem concluir que foi possível padronizar um teste diagnóstico, baseado na amplificação gênica alelo específica (PCR-AE) e na amplificação gênica multiplex (PCR-Multiplex). / The hemoglobinopathies are a group of hereditary hemoglobin disorders with worldwide distribution, meeting Brazilian population significantly; being decurrent from structural genes alterations, responsible for hemoglobin variants or in regulatory genes, results the thalassemia. The results permit us to conclude that it was possible to standardize a diagnostic test, based on allele-specific amplification (PCR-AE) and multiplex PCR assay. The applicability of these methodologies give us confidence on results interpretation, and it is easy of execution, and with cost around 25% less of methods that uses restriction enzyme analysis, and can offer us a laboratory diagnostic in a short time. The methodologies or association of obtained knowledge gave us the possibility to identify homozygous, heterozygous and interactions, and was possible to establish specific protocols to identify hemoglobinopathies that attacks our population, and can be used on laboratorial routines.

Traditional serologic assays of Actinobacillus pleuropeumoniae often have problems with cross-reactivity. To avoid the complications of antibody-antigen reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae and identify serotype 5 strains. Primers specific to the conserved capsular export region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the exception of serotype 4. A second set of primers specific to the unique capsular biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5 only. The sensitivity of this assay was determined to be less than 100 colony forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively distinguishes serotype 5 strains from other serotyes. / Master of Science

Traditional immunological assays used to serotype Actinobacillus pleuropneumoniae have been problematic due to cross- reactivity between serotypes, particularly serotypes 6 and 8. To avoid these serological cross-reactions, a multiplex PCR assay was developed to detect A. pleuropneumoniae and identify serotypes 1, 2, and 8. Primers specific to the conserved capsular polysaccharide export region of A. pleuropneumoniae serotype 5 amplified a 880 bp fragment in all serotypes excluding serotype 4 or a 489 bp DNA fragment in all serotypes including serotype 4. Primers specific to the capsular polysaccharide biosynthesis regions of A. pleuropneumoniae serotypes 1, 2, and 8 amplified a 1.6 kb, a 1.7 kb, and 970 bp fragment in the respective serotype. This PCR assay detects A. pleuropneumoniae and identifies serotypes 1, 2, and 8. / Master of Science