The purpose of this study is to investigate the presence of losses in a production department. Based on this analysis, improvements and suggestions that meet the performance requirements set by the company on the department’s planed cycle time. With this purpose in mind, two questions have been raised. These are the questions: 1. What losses can be identified in the department? 2. How can these identified losses in the department be reduced? A case study has been performed on a producing company, where the production has been observed and staff from the department has been interviewed for increased insight into the situation. The data presented has been analyzed in relation to the theoretical framework set up to achieve the results of the study. The result shows how the presence of losses in a production can be connected to production planning and batch size. These connections have in this study support in Lean production and its three M. Losses associated with waste were also found, such as movement, transport and waiting. In this study, there was only focus on one case company. The case company is a producing company, the department that the study focuses on produces a compressor product. The study does not take into account the production design or requirements beyond what is relevant for designing the improvement proposals. / Syftet med denna studie är att undersöka förekomsten av förluster på en produktionsavdelning. Utifrån denna analys ta fram förbättringsförslag som möter de prestandakrav företaget ställer på avdelningens planerade cykeltid. Med detta syfte i åtanke har det tagits fram två frågeställningar. Dessa frågeställningar lyder: 1. Vilka förluster kan identifieras på avdelningen? 2. Hur kan dessa identifierade förluster på avdelningen reduceras? Det genomfördes en fallstudie på ett producerande företag, där produktionen har observerats och personal från den studerade avdelningen har intervjuats för en ökad insikt i situationen. Den data som tagits fram har analyserats i förhållande till det teoretiska ramverk som ställts upp för att nå fram till studiens resultat. Resultatet visar hur förekomsten av förluster i en produktion kan kopplas till produktionsplanering och partistorlek. Dessa samband har i denna studien stöd i Lean Production och dess tre M. Förluster kopplade till slöseri hittades också, såsom rörelse, transport och väntan. I denna studie fanns endast fokus på ett fallföretag. Fallföretaget är ett producerande företag, där studien fokuseras på en avdelning som tillverkar en kompressorprodukt. Studien tar ingen hänsyn till produktions utformning eller kravspecifikation utöver det som är relevant för utformning av förbättringsförslagen.

The purpose of the project: The aim of this project was to finish the current BOM-list that already existed so it could be sent to X-ponent. When that was done it got possible to identify the non X-ponent products from other suppliers. These products had to be order by the needs to build walls, roof, and etc. When all the products were on place on the BOM-list the next goal was to send it back to X-ponent so they could buy them and then start to build different car-riages/boards by knowing who to call. The last step was to plan a delivery based on the most effective way by identify the risks. Make sure that the existing BOM-list is finished with the most suitable tools/ equip-ment’s and how to strategically buy them from X-ponent. How can X-ponent buy the non X-ponent products effective from the suppliers? How will these assembled products arrive effective from X-ponent to Husmutterns facil-ity?  How will these products be financed?Sum: A plan for the purchaser on Husmuttern AB so he/she just have to call X-ponent only once and to tell them a thought date for the products to be on place.Method: To achieve with these tasks, the author had to do a literature study on lean and six sigma tools. The comparison that was done between Husmuttern AB and XX gave a clear im-age about how respective companies purchasing process are. When these studies were done it became easier to handle these tasks, sush as fill in the rest on the BOM-list, knowing how transport the goods effective and also know what to take up on the attended meetings with the suppliers and to communicate through email and phone.Study results: The result for this project was to fill the required equipment’s/tools for the company that is thought to be purchased, considering the best products for these types of car-riage/boards. When all the tools/equipment’s were covered and finished the result was how to strategically buy them from this supplier (X-ponent). The author had to find the best logistics company to deliver these products and therefore was the delivery time estimated and founded based on Google maps estimated time to travel from Phils to X-ponent and then to Husmuttern AB:s facility. To transport the goods from X-ponents facility to the truck was estimated based on earlier experience out of the seller on X-ponent and CEO on husmuttern. The total price for each product from X-ponent:The exact price exkl moms:1 Instruction carriage/board: XXkr2 Tool/equipment carriages/boards: XXkr1 Charging carriage/boards: XXkr1 Unloading carriage: XXkr1 Unrolling stand: XXkr16 Pallet carriages: XXkrThe estimated price for the fixtures:1 Roof fixture: XXkr1 Wall fixture: XXkr1 Inner wall/inner roof fixture: XXkr1 Floor fixture: XXkr1 Corner fixture: XXkrThe estimated delivery price for the total transport: XXkrA total estimated price for the whole process: XXkr excl VATRecommendations: When the facility is on place and is producing the houses, it is important that the staff on the company evaluate the products that was bought, so if they know if any changes will be done or not. This will also help them to save money so they do not have to buy same tools/equipment over and over again. If you see it from another perspective, it will make4 (67)it possible for Husmuttern to tell X-ponent that the products they bought is not good as they thought and that will help X-ponent to fix the coming problems, and maybe make a discount to other Husmuttern facilities that probably be established in the coming future.It is also very im-portant that the company always looks what the market has to tell, and be very aware what the costumers needs in the coming future.

This thesis is focused on some ways of optimizing logistic processes. In the theoretical part are described important terms which are going to introduce the reader to the processes, their analysis followed by optimization. Then there is the space devoted to the metrics by which processes are possible to be evaluated and managed. The practical part offers case study. It contains introduction to the organization, mapping of their processes, analysis of warehouse processes, and their subsequent evaluation and proposed improvements. Suggested improvements were made in the spirit of Lean Management. As another proposal to improve warehouse processes an analysis of inventories have been made and evaluated, which resulted in proposal for new layout of the warehouse, which will encourage further optimization.

The thesis is concerned with logistic processes optimization in automotive industry companies that assemble car components. In the theoretical part of the thesis, areas and a structure of industrial logistics are defined and frequently used terms are explained. Then, theoretical approaches and practical applications used in the practical part are described. In the practical part, two particular companies are presented. The aim of the thesis is to optimize a tool that is used in the first organization during the process of logistic preparation of manufacturing. Secondly, the aim of the thesis is to analyse the process of material picking and handling before the supply of assembly line that is performed in the second organization and to suggest a solution that would eliminate non-value added activities. In the end, benefits of the thesis for both the organizations and the author are summarized.

The thesis deals with the application of Lean management in optimizing business processes, namely logistics processes. The author narrows the theoretical view as possible in today's practice to optimize the logistics processes in the real case study of a company. One of the theoretical foundations of modern Japanese approach based on Lean Management is called Toyota Production System. The practical focus of the work lies in analysis of the actual deployment of optimization in company specialized in hotel industry. Finally, thesis evaluates the success of optimization in the organization. The author has proposed some practical pieces of advice and techniques to approach optimization in near future.

This master´s thesis describes the production process in Japanese enterprise Daikin Device Czech Republic s.r.o. with emphasis on the production schedule, equipment and manufacturing system with elements of lean production. According to the production process analysis at the given production line a new solution of the production process has been suggested to increase production efficiency. This conception has been implemented and compared to the production process before, as well as to the assumed production process after increasing the efficiency.

A steady state level of negative supercoiling is essential for chromosome condensation, initiation of replication and subsequent elongation step. DNA gyrase, found in every eubacteria, serves the essential housekeeping function of maintenance of the negative supercoiling status of the genome. The functional holoenzyme is a heterotetramer, comprising of two GyrA and two GyrB subunits. DNA gyrase is an indispensable enzyme and serves as a readily susceptible target for natural antibacterial agents. The enzymatic steps of topoisomerisation by gyrase involve transient double strand break and rejoining of the strands after intact duplex transfer. Corruption of its catalytic cycle can lead to the generation of cytotoxic double-strand DNA breaks. Most of the anti-gyrase agents achieve their objective by targeting the vulnerable step of the reaction cycle i.e. DNA cleavage step. Bacteria on their part must have evolved and adopted strategies to counter the action of external agents and prevent the generation of double strand breaks thereby safeguarding their genome. In the present thesis, attempts have been made to understand the role of three endogenous gyrase interacting proteins in gyrase modulation and cellular defense against anti-gyrase agents. The thesis is divided into six chapters. Chapter 1 introduces the wonder enzymes “DNA topoisomerases” starting with a brief classification of these enzymes and their physiological functions. In the next section, DNA gyrase has been discussed in greater detail. The structural aspects as well as the mechanism of the topoisomerisation reaction catalyzed by gyrase have been discussed. Final section gives an overview of different gyrase modulators known till date focusing on their source, structure and mode of action. The scope and objectives of the present study is presented at the end of this chapter. In Chapter 2 is aimed at understanding the physiological role of GyrI. GyrI, originally identified in Escherichia coli as an inhibitor of DNA gyrase, has been previously shown in the laboratory to render protection against gyrase poisons and also various other DNA damaging agents (mitomycin C, MNNG). Abolishing GyrI expression renders the cell hypersensitive to these cytotoxic agents. Interestingly, GyrI exhibits contrasting behavior towards two plasmid encoded proteinaceous poisons of DNA gyrase. It reduces microcin B17-mediated double-strand breaks in vivo, imparting protection to the cells against the toxin. However, a positive cooperation between GyrI and F plasmid encoded toxin CcdB, results in enhanced DNA damage and cell death. These results suggest a more complex functional interplay and physiological role for GyrI. Search for other chromosomally encoded gyrase inhibitors led to YacG, a small zinc finger protein (7.3kDa) from E. coli, shown to be a member of DNA gyrase interactome, in a protein-protein interaction network described recently. Chapter 3 deals with the detailed characterization of YacG. It is shown that YacG inhibits DNA gyrase by binding to GyrB subunit and preventing DNA binding activity of the enzyme. More importantly, it protects against the cytotoxic effects of other gyrase inhibitors like ciprofloxacin, novobiocin, microcin B17 and CcdB. Further investigations revealed that YacG and its homologues are found only in proteobacteria. Hence, it appears to be a defense strategy developed by gram-negative bacteria to fight against the gyrase targeting cytotoxic agents. Inhibition by YacG appears to be specific to E. coli gyrase as mycobacterial enzyme is refractile to YacG action. GyrB, only in gram-negative organisms, possesses extra stretch of 165 amino acids, indispensable for DNA binding. Biochemical experiments with the truncated GyrB lacking the extra stretch reveal the importance of this stretch for stable YacG-GyrB interaction. E. coli topoisomerase IV is also resistant to YacG mediated inhibition, probably due to the absence of the extra stretch in ParE subunit, which is otherwise highly similar to GyrB. Further, YacG homologues from other proteobacterial members (Sinorhizobium meliloti and Haemophilus influenzae homologues sharing 35% and 63 % identity with E. coli YacG respectively ) also inhibits E. coli DNA gyrase at comparable levels. YacG thus emerges as a proteobacteria specific inhibitor of DNA gyrase. The occurrence of both YacG and the gyrase extra stretch only in proteobacteria, suggest co-evolution of interacting partners in proteobacteria. In Chapter 4, the study of endogenous gyrase modulators is extended to Mycobacterium sp. glutamate racemase (MurI) from E. coli has been shown earlier to be an inhibitor of DNA gyrase. However, nothing much was known about its mode of action. MurI is an important enzyme in the cell wall biosynthesis pathway, which catalyses the conversion of L-glutamate to D-glutamate, an integral component of the bacterial cell wall. In this chapter, it is demonstrated that M. tuberculosis MurI inhibits DNA gyrase activity, in addition to its precursor independent racemization function. The inhibition is not species specific as E. coli gyrase is also inhibited. However, it is gyrase specific as topoisomerase I activity remains unaltered. The mechanism of inhibition by MurI has been elucidated for the first time and it is shown that MurI binds to GyrA subunit of the enzyme leading to a decrease in DNA binding of the holoenzyme. The sequestration of the gyrase by MurI results in inhibition of all reactions catalyzed by DNA gyrase. Chapter 5 is the extension of the studies on glutamate racemase into another species, i.e. Mycobacterium smegmatis. DNA gyrase inhibition seems to be an additional attribute of some of the glutamate racemases, but not all, as Glr isozyme from B. subtilis has no effect on gyrase activity in spite of sharing a high degree of similarity with the gyrase inhibitory glutamate racemases. It is shown that like the M. tuberculosis MurI, M. smegmatis enzyme is also a bifunctional enzyme. It inhibits DNA gyrase in addition to its racemization activity. Further, overexpression of the enzyme in M. smegmatis provides protection to the organism against fluoroquinolones. DNA gyrase inhibitory property thus appears to be a typical characteristic of these MurI and seems to have evolved to either modulate the function of the essential housekeeping enzyme or to provide protection to gyrase against gyrase inhibitors, which cause double strand breaks in the genome. In the above chapters, it is shown that besides its crucial role in cell wall biosynthesis, mycobacterial MurI moon lights as DNA gyrase inhibitor. That the two activities exhibited by M. tuberculosis MurI are unlinked and independent of each other is demonstrated in Chapter 6. Racemization function of MurI is not essential for its gyrase inhibitory property as mutants compromised in racemization activity retain gyrase inhibition property. MurI- DNA gyrase interaction influences gyrase activity but has no effect on racemization activity of MurI. MurI expression in mycobacterial cells provides protection against the action of ciprofloxacin, thereby suggesting a role of MurI in countering external agents targeting DNA gyrase. Further M. tuberculosis MurI overexpressed in near homologous expression system of M. smegmatis yields highly soluble enzyme which can be further used for structural and functional studies. In conclusion, the studies reveal that the endogenous inhibitors essentially influence the enzyme activity by sequestering the enzyme away from DNA. None of them cause cytotoxicity, which usually arises as a result of DNA damage caused by accumulation of gyrase-DNA covalent intermediate. On the contrary they provide protection against such gyrase poisons. Comparative analysis of these proteinaceous inhibitors, however, does not reveal a common motif or structural fold, required for their ability to inhibit DNA gyrase. Based on these studies, it can be proposed that these endogenous proteins exist to serve as cellular defense strategies against external abuse and also to modulate the intracellular activity of DNA gyrase as and when required, for accurate division, functioning and survival of the cells.