La polarité est une question critique pour le système de matériaux III-nitrures, qui a un impact sur la qualité des films épitaxies et la performance des dispositifs à base de nitrure. Mais la compréhension des mécanismes élémentaires responsables de l'établissement de la polarité N ou métallique des films sur le substrat non-polaire manque. Les concepts existants sont basés sur des observations empiriques et contiennent des résultats ambigus. Une des raisons principales est le manque d'outils analytiques, permettant la détermination localisée de la polarité et de la structure atomique des couches à l'époque, lorsque les concepts de contrôle de la polarité ont été établis. Dans ce travail, nous développons un concept de contrôle de la polarité dans les couches AlN et GaN épitaxies sur substrat de saphir par EPVOM. La polarité des couches est étudiée par microscopie électronique en transmission (MET) haute résolution corrigée des aberrations et par microscope électronique à balayage en transmission en champ sombre (HAADF-STEM). L'analyse des investigations expérimentales donne les principaux résultats suivants : (i) le mécanisme qui régit la sélection de la polarité ; (ii) la relation entre la nitruration de la surface et les domaines de polarité Al dans les films d'AlN N-polaire ; (iii) possibilité d’inverser la polarité N de films d’AlN de polarité mixte en introduisant un recuit sous oxygène. La compréhension de mécanisme par lequel la polarité est contrôlée ouvre les possibilités d'une ingénierie de polarité dans les films de nitrure et peut donner une idée de la compréhension du contrôle de la polarité dans d'autres systèmes de matériaux (par exemple, les oxydes). / Polarity is a critical issue for III-nitrides material system that has an impact on the quality of epitaxial films and the performance of nitride-based devices. But the understanding of the elementary mechanisms that are responsible for establishing metal or nitrogen polarity of the films on nonpolar substrate is lacking. The existing concepts are based on empirical observations and contain ambiguous results. One of the main reasons for that is the lack of precise analytical tools, allowing localized determination of polarity and atomic structure of layers, at the time, when main concepts for polarity control were established. In this work we develop a concept of polarity control in AlN and GaN layers grown by MOVPE on sapphire substrates. The polarity of the layers is studied by aberration corrected HRTEM and high resolution high-angle annular dark field (HAADF) scanning TEM. The analysis of the experimental investigations yields the following principal results: (i) mechanism that governs polarity selection; (ii) relation between sapphire surface nitridation and Al-polar domains in N-polar AlN films; (iii) possibility of controlled switching the layers polarity from N to Al by oxygen annealing.Understanding of this mechanism by which polarity is controlled opens up the possibilities for polarity engineering in nitride films and can give a clue to understanding polarity control in other material systems (e.g. oxides).

Les septines sont des protéines conservées de la levure à l’homme qui sont impliquées dans divers processus cellulaires tels que la cytokinèse, le transport vésiculaire et l’organisation du cortex cellulaire. Il existe 13 gènes de septines retrouvés en plusieurs isoformes chez l’humain, et seulement cinq chez Drosophila melanogaster, Sep1, Sep2, Pnut, Sep4 et Sep5, ce qui en fait un modèle idéal vu son génome simple. Les septines sont composées d’un domaine de liaison au GTP très conservé entre les espèces, dont le rôle reste à ce jour ambiguë, ainsi que de régions N et C-terminales variables. Les septines s’assemblent entre elles pour former un hexamère, composé de Sep1, Sep2 et Pnut chez Drosophila melanogaster, via l’interface N-C et G des septines. Ces hexamères s’assemblent bout à bout afin de former les filaments de septines. Ces filaments peuvent ensuite se regrouper et s’assembler en structures hautement ordonnées telles que des anneaux, des tubes, des faisceaux de filaments, des cages et elles sont retrouvées au sillon de clivage durant la cytokinèse. Le but était de déchiffrer les mécanismes d’assemblage des filaments de septines qui mènent à la formation des différentes structures, afin de mieux comprendre les mécanismes d’interaction entre les septines. Au sein des cellules S2 de Drosophila melanogaster, les septines sont retrouvées à trois structures hautement ordonnées et dépendantes de Pnut endogène : des tubes cytoplasmiques, des anneaux cytoplasmiques et le sillon de clivage durant la cytokinèse. Notre hypothèse est qu’il existe plusieurs mécanismes qui régissent la formation des structures hautement ordonnées et que ceux-ci sont dépendants des régions N et C terminales variables des septines qui sont impliquées dans plusieurs interactions. Divers mutants de Sep1, Sep2 et Pnut tronqués en N et en C-terminal ont été fusionnés à une protéine fluorescente et caractérisés par microscopie confocale. La localisation de ces mutants a été répertoriée et analysée en présence des septines endogènes ou lors de la déplétion de celles-ci. Nos résultats suggèrent que le domaine de liaison au GTP est suffisant pour le recrutement des septines au sillon de clivage durant la cytokinèse, mais que la région N-terminale est requise la formation des tubes et des anneaux cytoplasmiques dépendants de Pnut. / Septins are conserved from yeast to humans and are implicated in diverse cellular processes such as cytokinesis, vesicular transport and cellular cortical organization. There are 13 known genes that encode for human septins, which also have many isoforms, while there are only five septin genes in Drosophila melanogaster: Sep1, Sep2, Pnut, Sep4 and Sep5, which makes it an ideal model system. Septins have a conserved GTP binding domain, whose role is still not fully understood, and variable N-C-termini. Septins assemble together, via N-C and G interfaces, to form a hexamer, that is composed of Sep1, Sep2 and Pnut in Drosophila melanogaster, which assemble end-to-end to form non polar filaments. These filaments can subsequently assemble together to form higher-ordered structures, such as rings, tubes, bundles, and gauzes. Furthermore, septins are recruited to the cleavage furrow during cytokinesis although their organization there is unclear. The aim of this project is to define septin assembly mechanisms that can lead to the formation of different higher ordered structures. In Drosophila melanogaster S2 cells, septins are recruited to three, readily observable septin dependent structures: cytoplasmic rings, cytoplasmic tubes, and the cleavage furrow during cytokinesis. Our hypothesis is that multiple mechanisms govern septin incorporation into these structures and that these mechanisms differentially depend on septin N-C variable termini. A panel of mutants of Sep1, Sep2 and Pnut truncated in N-C-termini were fused to fluorescent proteins and their localization in S2 cells monitored by confocal microscopy, with or without depletion of endogenous septins. My results suggest that the GTP binding domain is sufficient for septin recruitment to the cleavage furrow during cytokinesis, but that the septin N-termini are required for recruitment to the cytoplasmic tubes and rings.

This thesis deals with the development of new reaction methodology as well as stereochemical investigations. The first part concerns the investigation of 1,2- and merged 1,2- and 1,3- asymmetric induction in Mukaiyama aldol additions to α-heteroatom and α,β- heteroatom substituted aldehydes respectively. In particular, the unexpected 1,2-syn selectivity obtained in the addition of sterically hindered nucleophiles to α-chloroaldehydes is examined, and an explanation for the observed stereochemical trends is proposed. The second part describes the development of a novel entry to α-amino-β- hydroxy esters by a 1,3-dipolar cycloaddition reaction of aldehydes and azomethine ylides, generated by thermolysis of aziridines. The third part deals with our efforts to develop a novel entry to vicinal all- carbon quaternary centers, based on an intramolecular domino Heck- carbonylation reaction using tetrasubstituted olefins. / QC 20120611

The interaction between a bluff body and the impinging fluid flow, can involve detached boundary layers, massive flow separations, free shear layers, development of recirculation zones and formation of a highly disturbed and complex region downstream of the bluff body, which can be categorized as wake. The present research aims to experimentally investigate such fluid-structure interaction and provide insight into the wake structure of two bluff bodies. To this end, the airwake over the helicopter platform of a Canadian Coast Guard (CCG) polar icebreaker was studied using high-speed particle image velocimetry (PIV). The experiments were conducted on a scaled model of the polar icebreaker situated on a costume-built and computer-controlled turntable, which provided the ability to accurately change the incidence angle of the impinging flow with a given rate of change for incidence angle. Quantitative flow field data were obtained in several vertical and horizontal planes. The obtained velocity field was then used to calculate the time-averaged flow structure and turbulence metrics over the helicopter platform of the vessel. The present work compared the effects of two types of inflow conditions: (i) a uniform flow and (ii) a simulated atmospheric boundary layer (ABL) on the flow structure over the helicopter platform of the ship. Moreover, for the bluff scaled model, the effects of the Reynolds number on the wake structure and the flow patterns were investigated. The incidence angle (α) between the oncoming flow and the orientation of the ship varied between 0° to 330° with the increment of 30°. It was observed that higher maximum values of the turbulence intensity were associated with the simulated ABL. Moreover, it was found that for both inflow conditions, the incidence angle of 300o corresponded to the highest turbulence levels over the helicopter platform. Building on the results obtained for a stationary vessel in the simulated ABL, this work aimed to quantify the effects of the unsteady change in the direction of the impinging wind, simulated by rotating the model at a certain rate, . It was observed that the increase of the rate of change of the inflow direction resulted in an increase of the turbulent intensity over the helicopter platform. However, an exception was observed for the case of α = 60°, where clockwise rotation of the ship model with respect to the inflow exposed the helicopter platform to increased turbulent velocity fluctuations, while counterclockwise rotation diminished the flow unsteadiness over the helicopter platform. Moreover, aiming to identify the origins of the unsteady forces applied on bluff elongated plates with high chord-to thickness ratio (c/t = 23) at zero incidence, direct force measurement as well as PIV were used to identify the effect of transverse perforations on the flow-induced loading on the flow structure in the near-wake of the plates. The experiments were conducted in a water channel, where the plates were located at the center of channel, parallel to the upstream flow direction. Plates with various characteristic diameter of the perforation as well as a reference case without perforations were considered. The spectra of the trailing-edge vortex shedding and flow-induced forces were compared and it was observed that the vortex shedding frequencies were in very good agreement with those of the measured flow-induced forces for all considered perforation patterns. Thus, it was determined that the trailing-edge vortex shedding was the main mechanism of generating the unsteady loading on the plates. The staggered patterns of the perforations created a three-dimensional flow structure at the vicinity of the trailing edge and in the near wake, which was investigated using PIV at several data acquisition planes. It was found that in the cross-sectional planes corresponding to the close proximity of the perforations to the downstream edge, the periodic trailing-edge vortex shedding were suppressed. Furthermore, it was observed that for small perforations, the velocity fluctuations in the near wake were enhanced. However, further increase of the perforation diameter led to suppression of the velocity fluctuations. / Graduate

Chapter 1: Antarctic fishes are extremely cold adapted. Despite their inability to upregulate heat shock proteins, recent studies have demonstrated a capacity for heat response in these animals. A cDNA microarray study looked at the Notothenioid fish Trematomus bernacchii and revealed heat sensitivities for hundreds of genes, two of which code for members of the CCAAT/Enhancer-binding protein (C/EBP) family of transcription factors. These molecular switches are best known for their roles in apoptosis, inflammation and cell cycle arrest. This dissertation further elucidates the role of C/EBP-delta in the Antarctic fishes T. bernacchii and Pagothenia borchgrevinki. Chapter 2: C/EBP-delta is constitutively expressed in unstressed, field-acclimated (ca. -1.86°C) animals in a highly tissue-specific manner. White muscle tissue contains the highest C/EBP-delta concentration, which is further increased in response to sublethal heat stress at 2.0 or 4.0°C. This response is mostly acute and transitory, but a lesser upregulation was observed in fishes held for one month at 4.0°C. Chapter 3: The heat-induced nuclear translocation of C/EBP-delta--as determined by immunohistochemistry--appears to be time, tissue and species specific with spleen, heart and retinae being particularly responsive in certain situations. Chapter 4: Protein concentrations of proliferating cell nuclear antigen are tissue specific and variably heat responsive. Surprisingly, levels appear to be positively correlated with C/EBP-delta. Chapter 5: Flow cytometry revealed increasingly high temperatures reduce the proportion of G1 cells while increasing the abundance of apoptotic cells. Chapter 6: These findings are discussed in the context of global climate change and the cellular stress response.

The diploma thesis deals with the creation of map bases in the northern part of the Metra areal in Blansko in the form of a thematic map in a scale 1:250. Descriptive information for each element has been added to the map. The thesis describes in detail the process of creating a measuring network, detailed survey, calculations, testing accuracy and creation of a thematic map including descriptive information.

The diploma thesis deals with planimetric and altimetry determination of the area Metro company in Blansko , its southern part. The content of the thesis is a detailed description of the schedule, survey section, calculations, graphic processing in the GEOSTORE V6® program, description information connection. The result is a printed 1: 250 scale maps in the S-JTSK coordinate system and the Bpv elevation system.

No description available.

Die gestiegene Messgenauigkeit der Erdrotationsparameter ermöglicht inzwischen eine Berücksichtigung von Sekundäreffekten, wie Einflüssen hydrologischer Massenvariationen. Variationen der Erdrotation durch hydrologische Massenvariationen sind bisher weniger gut als atmosphärische und ozeanische Beiträge erforscht. Zur Interpretation, Analyse und Zuordnung von Signalen in den Erdrotationsparamtern wurden mit einem Landoberflächenschema und einem lateralen Abflussmodell langjährige hydrologische Simulationen unter Antrieb aus verschiedenen globalen Atmosphärenmodellen durchgeführt. Diese Simulationen erlaubten die Durchführung von Sensitivitätsstudien über die Abhängigkeit simulierter hydrologischer Beiträge zur Erdrotation von den atmosphärischen Antrieben und der Modellphysik. Alle relevanten hydrologischen Größen wurden auf Plausibilität mit Beobachtungsdaten oder anderen Simulationsergebnissen validiert. Neben dem Beitrag der vertikalen Wasserbilanz wurde auch der Beitrag des lateralen Wassertransportes auf die Erdrotation explizit untersucht. Erstmalig konnte der hydrologische Bewegungsterm quantifiziert werden. Die simulierten hydrologischen Drehimpulsfunktionen wurden schließlich im Kontext des globalen Wasserkreislaufes den beobachteten Erdrotationsparametern gegenübergestellt. Es ergab sich eine besonders gute Übereinstimmung für die hydrologische [chi2]-Komponente mit Residuen aus Beobachtungen und simulierten atmosphärischen und ozeanischen Variationen der Erdrotation. / The increasing accuracy of observation of the Earth's rotation allows the consideration of so called secondary effects like the influence of hydrological mass redistributions. Variations of the Earth's rotation due to hydrological influences are yet less investigated than atmospheric and oceanic contributions. In order to analyse, interprete and associate signals in the parameters of the Earth's rotation, longtime hydrologic simulations were performed by forcing a land-surface scheme and a lateral discharge model with data of various global atmospheric models. By these simulations, the dependency of simulated hydrologic contributions to the variations of the Earth's rotation on atmospheric forcing and on the model physics was studied. All relevant hydrologic quantities were validated against observed or modelled values to evaluate their plausibility. In addition to the contribution of the vertical water balance to the variations of the Earth's rotation also the contribution of lateral water transport was explicitely investigated. For the first time the hydrologic motion term was quantified. Eventually, the simulated hydrological angular momentum functions were compared in the context of the global water cycle against observed parameters of the Earth's rotation. The hydrological component of [chi2] did show a very good agreement with residuals of observations and simulated atmospheric and oceanic variations of the Earth's rotation.

Using flake dimensions and attributes commonly agreed are associated with site use, occupation age, and occupation duration, it was argued that relative estimations of site function and occupation age could be determined using debitage. This is particularly beneficial for assemblages that have little to no diagnostics that could provide a general cultural period for one or more occupations at a site. The results of this study suggest that, although certain attributes are generally associated with lithic production stage, relative age, and duration indicators, they were not all applicable within this study. The methods employed were relatively successful; however, reducing the number of classes, removing of a dimension, and more sites that meet the definition of lithic scatter is needed. Furthermore, testing occupation duration using the number of breaks on a flake is not possible unless it is proven a single occupation site.