Corn (Zea mays L.) intercropped with Brachiaria spp. plays an important role in tropical agriculture management, providing residues or forage to areas intensively cropped. Although previous studies provide useful information about effects of intercropping on soil management and crop yield, a better understanding of how corn-Brachiaria intercropping systems impacts exogenous and endogenous nutrient dynamic is needed. Two experiments were performed in the both conventional and late planting season. In the first, corn and palisadegrass (Brachiaria brizantha cv. Marandu) monoculture were compared with two intercropping patterns, directed for production of residues or forage, with simulated animal grazing. Biomass, crop nitrogen (N) content, N derived from fertilizer and N budget were measured. Among farming systems, variable responses remained almost unchanged during period of simultaneous growth. After corn harvest, intercropping patterns achieved greater biomass (0.6-11 Mg ha-1) and N content (12-318 kg ha-1) relative to fallow preceded by corn monoculture, but it results in overall gains (pre and post-harvest) only to conventional planting season. N fertilizer recovery was not affected by intercropping patterns. When corn and Brachiaria were intercropped to establish pasture, simulated grazing after grain harvest had a tightly influence on N budget, approximately -221 kg ha-1. The second experiment investigated the influence of Brachiaria species on corn nutrients partitioning and their cycling after corn harvest. Biomass, N, phosphorus (P) and potassium (K) content were evaluated using corn intercropped with three species of Brachiaria (B. Brizantha cv Marandu, B. ruziziensis, and B. hybrid cultivar Mulato II, Convert HD 36) and corn monoculture. Biomass and nutrient content was not affect when corn was intercropped with Brachiaria species. After corn harvest, Brachiaria nutrient content and biomass yield were ranking from greater to lower following the order: B. brizantha > B. ruziziensis > B. convert. Relative with the corn monoculture, intercropping treatments enhanced the total biomass (corn + Brachiaria) and the nutrient accumulation only when planted at conventional season. Over 6 site-yr, benefits of intercropping appear after corn harvest, particularly at conventional planting season. Corn intercropped with Brachiaria species either had no effect N fertilizer recovery or affect N-fertilizer distribution within components of soil-plant system. Nonetheless, intercropping provide greater biomass accumulation after harvest compared with corn monoculture, resulting in larger nutrient content stored in plant component. Our results suggest a larger N requirements when crop-livestock activities was integrated. Corn intercropped with B. brizantha during conventional season was the best approach to enhanced crop yield and nutrient cycle for corn production systems. / O consórcio entre milho (Zea mays L) e espécies de Brachiaria spp. (syn. Urochloa spp.) desempenha importante função no manejo da agricultura tropical, produzir resíduos ou forragem em áreas intensivamente cultivadas. Apesar de estudos anteriores fornecerem valiosas informações sobre efeitos do consórcio sobre o manejo do solo e produção de biomassa, um melhor entendimento de como este sistema de cultivo afeta a dinâmica de nutrientes (exógenos ou endógeno) é necessário. Dois experimentos foram conduzidos durante a safra e segunda safra. No primeiro, os monocultivos de milho e braquiária (Brachiaria brizantha cv. Marandu) foram comparados com dois padrões de consórcio, direcionado a produção de resíduos ou forragem, com simulação do pastejo animal. Biomassa, acúmulo de nitrogênio (N), N- fertilizante e balanço de N foram avaliados. As variáveis respostas praticamente não foram afetadas pelos sistemas de cultivos durante o período de coexistência. Após a colheita do milho, os sistemas consórciados acumularam mais biomassa (0,6 -11 Mg ha-1) e N (12-318 kg ha-1) comparado ao pousio precedido pelo monocultivo de milho, apesar destes incrementos afetarem apenas o acúmulos totais (pré+pós-colheita) do cultivo de safra. A recuperação do N-fertilizante foi semelhante entre os sitemas de cultivo. Quando o consórcio objetivou a implantação de pasto, o pastejo simulado após a colheita do milho influênciou o balanço de N, com deficit de aproximadamente -221 kg ha-1.O segundo experimento foi conduzido para investigar a influência das espécies de Brachiaria sobre o particionamento de nutrientes no milho e a ciclagem pós-colheita de grãos. Produção de biomassa, quantidades de N, fósforo (P) e postássio (K) acumuladas foram avaliadas no monocultivo de milho e em três espécies de Brachiaria spp. (B. Brizantha cv Marandu, B. ruziziensis, and B. hibrido cultivar Mulato II, Convert HD 36) em consórcio com milho. Biomassa e acúmulo de nutrientes não foram afetados no consórcio do milho com as espécies de Brachiaria. Após a colheita do milho, o acúmulo de biomassa e nutrientes das espéceis de braquiária classificadas em ordem decrescente foram: B. brizantha > B. ruziziensis > B. convert. Comparado ao monocultivo de milho, o consórcio aumentou a biomassa total (milho+braquiária) e o acúmulo de nutrientes apenas quando cultivado na safra. Entre os 6 locais/anos, os benefícios do consórcio ocorreu após acolheita do milho, especialmente para o cultivo de safra. O milho consorciado com espécies de Brachiaria não afetou a recuperação do N-fertilizante nem sua distribuição nos componentes do sistema solo-planta. Entretanto, o consórcio proporcionou maior acúmulo de biomassa após a colheita do milho se comparado ao pousio precedido pelo monocultivo de milho, aumentando a porção de nutrientes alocados no compartimento planta do sistema. Nossos resultados sugerem que a integração entre atividades de lavoura e pastejo aumenta a demanda por N do sistema de produção. O consórcio entre milho e Brachiaria brizantha cv Marandu no cultivo de safra foi a melhor estratégia para aumentar a produção de biomassa e a ciclagem de nutrientes no sistema de produção do milho.

[Truncated abstract] Globally, tropical rainforests are noted for their high biodiversity and key roles in carbon storage and influence on climate. Nevertheless, tropical deforestation in many parts of the world continues at an alarming rate. In Papua New Guinea (PNG), tropical rainforest is relatively well maintained, with about 70 % of the land area still covered by primary forest. However, PNG's native forests are coming under increasing pressure, particularly from selective logging for high quality timber. While the forests of PNG, and more broadly the entire New Guinea Island, are recognised as of high conservation and ecological significance, they remain grossly understudied with little knowledge of key ecosystem processes within lowland forests in particular. Such knowledge is urgently required if the impacts of logging and other land-use change are to be assessed and in order to develop sustainable management systems. This thesis investigated the impacts of logging on diversity and nutrient cycling in a lowland tropical rainforest growing on limestone soils in the area of the Mongi-Busiga Forest Management Agreement (FMA, which is a logging concession area), in northeastern PNG. These forests are on relatively young soils and provide a useful contrast to the majority of tropical forests. The research includes a four-year study of the recovery of diversity and structure after logging, and quantified forest structure, tree species diversity, forest biomass and productivity, and nutrient distribution and cycling. This thesis also examines the ecological sustainability of Eucalyptus deglupta plantations in Wasab, PNG as an alternative resource for timber and biomass energy. The thesis concludes with a discussion of long-term forest recovery and sustainable forest management in north-eastern PNG. Two adjacent one-hectare plots were established in lowland tropical rainforest at Mongi-Busiga FMA. One of these plots was subsequently selectively logged, one year after establishment. Before logging, the two one-hectare plots contained a total of 37 families, 70 genera and 110 tree species that were >5 cm in diameter at breast height. Mean basal area was 42.4 m2 ha-1. Two tree species, Madhuca leucodermis (Sapotaceae) and Pometia pinnata (Sapindaceae) accounted for ~60% of the total basal area. Gymnacranthera paniculata (Myristicaceae) was the most common species and accounted for 13% of individuals. ... This study concludes that the Mongi-Busiga forest has many unusual characteristics for a tropical forest, including relatively low diversity of tree species, high accumulation of P in the biomass, and N limitations, compared to other tropical rainforests. However, those extraordinary characteristics may be explained well by the underlying geology of young, marine-derived limestone. Sustainable management of the lowland tropical forests of PNG should consider the consequences of logging on nutrient cycling processes, with the possible significant removal of P from site with repeated logging, as well as the interactions between N and P in these systems. Establishment of Eucalyptus plantations on previously cleared land also has the potential to meet some of the timber and biomass energy requirements of northern PNG in ecologically sustainable manner.

The incorporation of cereal straw/stubble often immobilises nitrogen (N). This can help conserve N in soil in organic forms, thus reducing loss through leaching over dormant winter periods. However, N-depressions that arise during decomposition can reduce crop yield. The inclusion of a legume green manure can supply fixed-N, thus alleviating the low N availability to crops. In this study, the effect of lupin (Lupinus angustifolius L.) green manure incorporation on barley (Hordeum vulgare L.) straw/stubble decomposition, and N availability was investigated. A field experiment was used to determine the effects of the green manure on decomposition. Decomposition of straw/stubble was monitored using the litterbag technique. Following green manure incorporation, soil cores were incubated in a glasshouse to determine mineral-N availability. Though not significant, the inclusion of lupin green manure seemed to increase the decomposition of straw/stubble during the growth period, then slowing it after its incorporation at 110 d. This was described by a logarithmic pattern of loss of - 4.97 g AFDW residue day⁻¹, with 60% remaining after 140 d. Treatments without lupin had a linear decomposition of - 0.12 g AFDW residue day⁻¹, with 49% remaining after 140 d. The loss of cellulose confirmed the differences in decomposition with the inclusion of lupin resulting in 2.79% less cellulose remaining in straw/stubble after 140 d compared to its exclusion. Lupin significantly increased pot oat N uptake and DM yield by 55 % and 46 %, respectively, compared to its exclusion. However, this effect was not observed in field sown wheat yields and the soil mineral-N measurements made. This study showed that the potential of lupin to increase straw/stubble decomposition by improving the retention and availability of N, leading to long-term yield benefits, needed further investigation.

The Tropical savannas of central Brazil (cerrado) and the Everglades wetland (Florida) ecosystems are ideal systems to study landscape spatial mosaics and their interactions. Both ecosystems show a variety of plant physiognomies distributed within small spatial scales and elevation gradients. Such variety of plant physiognomies provide an opportunity to investigate the roles of climate, topography, nutrient availability and water table dynamics as determinants of plant physiognomic distributions, and their role in shaping regional systems. South Florida Wetlands and the tropical savannas of central Brazil are examples of hydrologically-controlled ecosystems. In hydrologically-controlled ecosystems water sources, the availability of nutrients, and the patterns of water movement play important roles in determining vegetation structure and function. The main objective of this study was to understand ecosystem level processes that shape different physiognomies in two hydrologically-controlled ecosystems. I conducted field work at the IBGE ecological reserve, a field experimental station located in Brasilia, Brazil. I also worked at the Everglades National Park in an area located near the south entrance of the Park in Homestead, Florida. I carried out three interconnected studies investigating water and nutrient dynamics: (1) In a Brazilian savanna I manipulated levels of litter input and measured changes to soil properties, organic matter decomposition and tree growth. I found that changes in litter input affect soil physicochemical properties and soil biochemical processes. I also found that litter dynamics influence tree growth through their effects on soil physicochemical properties. (2) I also studied the effect of water table depth and its temporal variation on spatial patterns of vegetation distribution in the cerrado landscape. I monitored diurnal and seasonal changes in water table depth along two tree-density and topographic gradients. In addition, I measured woody species composition, growth rates of four tree species, litter production, soil nutrients, and nutrient resorption efficiency along those two gradients. I found that water table depth has an important role in determining the spatial distribution of cerrado physiognomies; it also affects tree growth, species composition and nutrient resorption efficiency. (3) In the Everglades I studied patterns of underground water uptake by two vegetation types. I monitored seasonal and diurnal changes in water table depth in a Hammock forest, in a stand dominated by the invasive woody species Schinus terebinthifolius, as well as the water level in an adjacent lake. I estimated stand level transpiration using two different approaches: with sap flow measurements and diurnal oscillations in water table levels. Then, I calculated the total quantity of groundwater withdrawn by evapotranspiration for the wet and dry seasons in the Hammocks and in the exotic invaded site and then compared the results. I found that water uptake by Everglades trees is well coupled to diurnal changes in water table depth and that the amount of water withdrawn from the groundwater was larger during the wet season than during the dry season. Finally, I detected hydrological feedbacks between different vegetation types and nearby bodies of water. Results of this study contributes to the current knowledge of ecosystem level processes in tropical and subtropical ecosystems where water circulation and water availability play a dominant role in shaping vegetation structure and function.

O cultivo de plantas de cobertura como cultura de inverno para produzir palha e proteger o solo após a colheita da cultura de verão, na região do Cerrado brasileiro, tem sido um dos maiores desafios para os produtores que optaram pela adoção do sistema semeadura direta. Outra dificuldade inerente ao sistema semeadura direta, com peculiaridade para o Cerrado, refere-se ao manejo do nitrogênio (N) na cultura do milho, já que o ciclo deste nutriente é extremamente dependente das condições climáticas, apresentando, por isto, comportamento diferente da região Sul do país. Nesta situação, a aplicação de N na semeadura da cultura de cobertura de solo com o objetivo de aumentar a produção de palha ou em pré-semeadura da cultura principal, buscando otimizar tempo, pode ser uma estratégia que apresente a mesma eficiência da fertilização na cobertura no milho em sucessão à soja. Os objetivos do estudo foram avaliar a produção de massa de matéria seca de culturas de cobertura de solo e de espécies espontâneas; determinar a influência das épocas da fertilização nitrogenada nos caracteres fenológicos, na produtividade de grãos e de massa de matéria seca da cultura do milho cultivado após 2 anos de soja e, também, quantificar a eficiência da adubação nitrogenada, utilizando a técnica da diluição isotópica (15N). Foram desenvolvidos dois experimentos na região do Triângulo Mineiro, no Estado de Minas Gerais, avaliando-se doses, fontes e épocas de aplicação de N. As doses de N foram: 110 kg ha-1 no experimento em solo de textura média e 70 kg ha-1 no experimento em solo de textura muito argilosa, como dose suplementar à adubação de semeadura. As culturas de cobertura de solo, cultivadas após a colheita da cultura de verão, não foram eficientes na produção de palha; a adubação nitrogenada aplicada na semeadura da cultura de cobertura de solo também não resultou em produção de palha. No experimento em solo de textura média houve resposta à aplicação de N em relação à testemunha e beneficio do parcelamento em diferentes épocas, em relação à aplicação de maiores doses em uma única aplicação. Neste experimento, não houve diferença na produtividade de grãos na fertilização de cobertura do milho, usando uréia ou sulfato de amônio. Em solo de textura muito argilosa não houve resposta na produtividade de grãos do milho quando se aplicou N suplementar à adubação de semeadura, independente da época de aplicação. A eficiência da adubação nitrogenada variou de 26 a 70% para as diferentes épocas de aplicação, sendo que a média de aproveitamento foi de 50%. A aplicação antecipada não se mostrou eficiente em relação à recuperação do fertilizante, sendo recomendada adubação em cobertura, principalmente em solos mais arenosos / Cultivation of soil cover crops as second crop willing to produce straw and protect the soil after the harvest of the summer crop at the Brazilian Cerrados region had been one of the biggest challenges faced by farmers that decided for the no tillage system. Another problem inherent to the no tillage system in the Cerrados in relation with the nitrogen management (N) for corn crops is due to the cycle of this nutrient is extremely dependent on climatic conditions and for this reason, a totally different activity in comparison with Southern region of Brazil must be considered. In this situation, N supply whether in the seeding time of the soil cover crops in order to increase the straw production or before the of main cultivation (pre-seeding) looking to optimize time, it might be a strategy that demonstrates the same efficiency offered by the topdressing of the corn in soybean succession. The objectives of the study were to evaluate dry matter production in the soil cover crops and spontaneous species; to determine the influence of different timing of N-fertilizer supply in the phenology characters, yield and dry matter of corn after 2 years of soybean cultivation and, also, to quantify the efficiency of the nitrogen supply using the isotopic dilution technique (15N). Two experiments were carried out at the Triângulo Mineiro region, Minas Gerais State, Brazil, where rates, sources and timing of N applications were evaluated. The rates of N were: 110 kg ha-1 for the sandy clay loam soil experiment and 70 kg ha-1 for the very clayed soil experiment, besides the nitrogen supplied at the corn crop seeding. The cultivation of the soil cover crops after the harvest of the summer crop was not efficient for cover straw production; the nitrogen supplied at the seeding time of the cover crops also did not result in cover straw production. On the sandy clay loam experiment there was response to nitrogen regarding to the control treatment and also pointed benefit using split time application in relation with higher dose n a unique application. Also there was no difference in the corn yield whether by using urea fertilization or ammonium sulfate topdressing. For the very clayed soil experiment, there was no response on corn yield when nitrogen was supplied at the seeding independent of application timing. The efficiency of the nitrogen fertilization ranged from 26 to 70% among the different application timing and the average efficiency was 50%. The anticipated application did not demonstrate efficient with regard to the fertilizer recovery, being recommended topdressing applications, especially in sandy soils

Para identificar a importância ecológica das matas ciliares é essencial conhecer a interação entre sua hidrologia e ciclagem de nutrientes. Isto se torna ainda mais crucial diante das crescentes transformações na paisagem causadas pelo homem, que têm promovido forte antropização destas formações florestais. O estudo foi conduzido em uma floresta ribeirinha sazonalmente alagada na região sudoeste da Amazônia entre os anos de 2005 e 2007. Foram medidas as principais vias hidrológicas: chuva, precipitação interna, escoamento pelo tronco, escoamento superficial, solução do solo e água subterrânea, além do rio Urupá. Em todas foram realizadas análises químicas para determinação das concentrações de C, N e macronutrientes (cátions/ânions). A mata ciliar apresenta solos ácidos, bem estruturados, pobres em nutrientes e com um eficiente mecanismo de retenção nas camadas superficiais, associado à distribuição da matéria orgânica, absorção por raízes finas ou retenção pelos complexos de troca do solo. Os estoques de nutrientes no solo estão dentro da faixa de valores observada em outros estudos na Amazônia, apresentando baixa fertilidade. A região estudada apresenta altos índices pluviométricos (em torno de 2125 mm), com um regime de sazonalidade bem definido (apresentando déficit hídrico entre maio e setembro). A partição da chuva na mata ciliar ocorre com 15% de interceptação pelo dossel e o restante sendo distribuído entre a precipitação interna (83%) e escoamento pelo tronco (2%). A lixiviação de nutrientes pelo dossel foi observada para a maioria dos elementos avaliados, excetuando Na+ e Cl-, confirmando o importante papel do dossel em suprir nutrientes para a mata ciliar. Este enriquecimento também é influenciado pela queima de biomassa durante a transição do período seco para o chuvoso. A contribuição do escoamento pelo tronco foi essencial, principalmente para NO3- e cátions básicos, evidenciando a importância da inclusão desta via hidrológica na rotina de amostragens em estudos de ciclagem de nutrientes em florestas. Os fluxos no solo foram maiores na camada superficial, principalmente em função da entrada da rica solução da precipitação interna, sendo sua contribuição extremamente alta para K+, Carbono Orgânico Dissolvido (COD), PO43-, NH4+ e SO42-. Alguns elementos apresentaram indícios de lixiviação durante os meses mais chuvosos, enquanto o Na+ apresentou lixiviação durante o ano inteiro, em função da natureza conservativa deste elemento. A retenção das bases observada no solo pode estar associada com a absorção pelas raízes, adsorção pelas fases minerais e orgânicas do solo ou ainda pela retenção de ânions, que também foi observada. A relação entre os íons e a descarga do rio Urupá gerou uma histerese em sentido horário, indicando uma importante contribuição do fluxo lateral (escoamento superficial), e evidenciando a importante conectividade entre a mata ciliar e o rio Urupá. A maioria dos elementos apresentou balanço positivo (Ca2+, K+, HCO3-, Cl-, SO42- e COD) ou próximo do equilíbrio (Mg2+, NH4+, NO3-, PO43-), com exceção do Na+. Estes resultados indicam que a floresta estudada apresenta um eficiente mecanismo de conservação dos nutrientes em seus solos, uma baixa contribuição da ciclagem geoquímica (intemperismo de rochas) e forte controle da atmosfera e dossel florestal caracterizando um ciclo relativamente fechado / To identify the ecological importance of riparian forests is crucial to evaluate the interactions between its hydrology and nutrient cycling. This becomes more important due to fast changes in landscape promoted by mankind, which has been causing strong anthropization of these forests. The present study was conducted on a seasonal flooded riparian forest in the Southwestern Amazonia between 2005-2007. Main hydrological flowpaths (rainfall, throughfall, stemflow, overland flow, soil solution, groundwater and the Urupá riverwater) were sampled and posteriorly analyzed for C, N and macronutrients (cations/anions). Riparian forest soils are acid, well structured, poor in nutrients and with an efficient retention mechanism at the surface layers, which is linked to organic matter distribution, fine roots absorption, or retention by soil exchange complexes. Soil nutrient stocks are within the range of values usually observed in other studies in the Amazon region, revealing low fertility. The studied region exhibit high annual rainfall (around 2125 mm), with a marked seasonality (drought stress from May to September) and rain partitioning in the riparian forest divided into 15% of canopy interception and the remaining distributed between throughfall (83%) and stemflow (2%). Nutrient leaching from the canopy was observed for most elements, except Na+ and Cl-, confirming the important role of the canopy in supplying nutrients to the riparian forest. This enrichment is also influenced by biomass burning during the transition between dry to wet season. Stemflow contribution was essential, especially for NO3- and base cations, showing the necessity to include this flowpath in routine sampling in nutrient cycling studies. The highest soil fluxes were observed at the surface layer as a result of enriched inputs from throughfall, especially for K+, Dissolved Organic Carbon (DOC), PO43-, NH4+ e SO42-. Some elements exhibited leaching during wetter months, whereas Na+ has leached throughout the year, as a function of the conservative nature of this element. Base retention in soils may be linked to root absorption, sorption by organic and mineral soil phases, or anions retention, which was also observed in this study. The relation between ions and Urupá river discharge exhibited a clockwise hysteresis, suggesting an important lateral flow (overland flow) contribution and the connectivity between the riparian forest and Urupá River. Most elements had positive budgets (Ca2+, K+, HCO3-, Cl-, SO42- e COD) or were close to neutral balance (Mg2+, NH4+, NO3-, PO43-), except Na+. The results point out efficient nutrient retention mechanisms in these soils, low contribution from geochemical cycling (rock weathering) and a strong control from the atmosphere and forest canopy, characterizing a relatively close nutrient cycling

Les invasions biologiques sont une cause majeure de perte de biodiversité à l’échelle mondiale. L’impact direct des espèces exotiques envahissantes (EEE) sur la structure et la composition des communautés a été bien documenté. Par contre, leur impact sur le fonctionnement des écosystèmes a été comparativement moins étudié. Dans le présent travail, j’ai mesuré sur le terrain et en conditions expérimentales l’impact de 7 espèces de plantes exotiques particulièrement envahissantes en Europe sur les propriétés chimiques du sol, sur la productivité et sur le stock d’éléments minéraux dans la biomasse. Malgré la diversité des groupes fonctionnels considérés dans notre échantillon (des espèces annuelles aux arbres), des impacts récurrents ont pu être mis en évidence. Les EEE ont toujours une biomasse et une productivité supérieures à celles de la végétation qu’elles envahissent. Il en va de même pour le stock d’éléments minéraux dans la biomasse aérienne. En ce qui concerne le sol, les traits fonctionnels des espèces ne permettent pas de prédire la direction et l’amplitude des impacts. Par contre, ces impacts sur le sol se sont révélés partiellement prévisibles en fonction des conditions écologiques initiales (contexte pédologique). La disponibilité des cations et du P et la concentration en C et N organiques augmentent suite à l’invasion dans les sites initialement pauvres alors que ces mêmes paramètres baissent dans les sites plus eutrophes au départ. Les EEE entraînent, dans une certaine mesure, une homogénéisation des conditions écologiques dans les écosystèmes envahis. <p>Dans un deuxième temps, je me suis attaché à identifier les mécanismes de l’impact de Fallopia japonica sur le cycle de l’azote. Cette espèce, perenne rhizomateuse, adopte une gestion très conservatrice de l’azote en retransloquant jusqu’à 80 % de l’azote des tiges et des feuilles vers le système racinaire en automne avant l’abscission des feuilles. La litière restituée est, de ce fait, pauvre en azote. Ce paramètre, combiné à une teneur élevée en lignine, explique sa faible vitesse de décomposition par rapport à celle de la végétation non envahie. Une grande partie de l’azote de l’écosystème tourne donc en cycle quasi fermé entre les organes de réserve et les parties aériennes de la plante, tandis que l’azote restant est en grande partie bloqué dans la nécromasse de la plante et est donc non disponible pour les espèces concurrentes indigènes. Ce mécanisme explique, sans doute, en partie le succès invasif de l’espèce.<p><p>A l’avenir, les implications des impacts sur le sol en terme de restauration des communautés devront être étudiées. L’hypothèse d’une influence positive de ces impacts sur l’aptitude compétitive des espèces envahissantes devra être testée. Enfin, une étude plus fonctionnelle des impacts de ces espèces dans des sites contrastés devrait permettre d’identifier les mécanismes impliqués./Biological invasions are a major cause of biodiversity loss worldwide. The direct impact of alien invasive species (AIS) on community structure and composition has been well documented. On the other hand, their impact on ecosystem functioning has been comparatively less studied. In this work, I measured, on the field and in experimental conditions, the impact of 7 highly invasive alien plant species in Europe on soil chemical properties, biomass and aboveground nutrient stock. Despite the high diversity in considered functional groups (from annuals to trees), recurring impacts have been found. AIS had always higher biomass and aboveground nutrient stocks than invaded resident vegetation. Concerning soil, species functional traits did not allow us to predict impact direction and intensity. On the other hand, impacts on soil were partially predictable based on initial ecological conditions (pedologic context). Thus, cations and P availability and organic C and N concentrations increased in initially poor sites and decreased in eutrophic ones. AIS tend to homogenize soil properties across invaded landscapes.<p>In a second part, i tried to identify the mechanisms of the impacts of Fallopia japonica on N cycling. This perrennial rhizomatous species manage N in a very conservative way. About 80 % of aboveground N is translocated to rhizomes before leaves abscission. The litter is therefore poor in N. This parameter combined with a high lignin concentration explains its slow decomposition rate compared to that of uninvaded vegetation. A big part of the ecosystem N is engaged in a nearly closed cycle between above and belowground organs while the remaining N is blocked in the necromass and is thus not available for the indigenous competitors. This mechanism probably explains the invasive success of this species.<p><p>In the future, the implications of soil properties modifications in terms of community restoration should be studied. The hypothesis of a positive influence of these modifications on the competitive ability of AIS should be tested. At last, a functional study of these soil impacts in contrasted sites should allow us to identify the implied mechanisms.<p> / Doctorat en Sciences / info:eu-repo/semantics/nonPublished

L'objectif de cette étude est de mieux comprendre comment la biodiversité influence le service de purification de la qualité de l'eau en tant que service de régulation capable de limiter la charge en polluants de l'eau naturelle. Peu d'études ont regardé comment les invertébrés (macro- et méio-faune) sont capables d'influencer le fonctionnement de la zone hyporhéique considérée, comme un réacteur biogéochimique contribuant largement au recyclage des nutriments. L'élimination du nitrate et la dénitrification sont utilisés comme indicateur de ce service dans les rivières afin de pouvoir suivre son évolution spatiale et temporelle. Dans cette thèse, la relation fonctionnelle entre le taux de réduction des nitrates et les organismes participant à l'expression de ce service est testée à différentes échelles d'étude allant du microcosme jusqu'à l'habitat hyporhéique d'un méandre de large rivière, la Garonne. Cette relation est mise en évidence dans une série de colonnes d'infiltration reproduisant le lit de rivière avec sa communauté benthique (projet Inbioprocess). Dans cette expérience, un gradient de biodiversité a été créé avec des combinaisons de communautés +/- biofilm, +/- méiofaune et +/- macrofaune pour tester leur influence sur l'élimination du nitrate avec et sans pesticides dans le cadre du projet Inbioprocess. Les résultats suggèrent l'influence des interactions entre communautés, sur le taux de réduction des nitrates qui est supérieur quand les invertébrés sont présents (11.8 ± 1.2) par comparaison avec les conditions sans invertébrés (7.7 ± 1.4 mg N l-1d-1 ; moyenne ± erreur standard (m ± ET)). Ces interactions ont également été suggérées comme favorisant le retour de la capacité de réduction des nitrates en présence de pesticides, utilisé comme source de stress, dans l'eau des microcosmes. Ces résultats de laboratoire montrent l'influence des interactions trophiques et non trophiques entre les différents niveaux trophiques de ce réseau, avec probablement l'implication des espèces les plus résistantes pour expliquer la capacité potentielle de résilience du système. L'existence de cette relation fonctionnelle de type "top-down" a ensuite été explorée en conditions in situ. Les taux de rétention mesurés dans 9 cours d'eau européens (projet STREAMES) ont été estimés à l'échelle du tronçon de rivière à 1.64 ± 2.39 (m ± ET) mg NO3--N m-2.min-1. L'influence des communautés d'invertébrés sur le taux de réduction des nitrates se révèle statistiquement comme aussi importante que celle des facteurs physicochimiques dans l'ensemble des tronçons explorés. L'étude des traits biologiques des communautés d'invertébrés a permis de préciser le type de communauté le plus corrélé aux processus d'élimination des nitrates. Ces organismes sont majoritairement interstitiels vivant dans les sédiments grossiers et avec des modes d'alimentation de type brouteurs de biofilm. Dans la zone hyporhéique de la zone humide alluviale de Monbéqui (projet Attenagua), la corrélation positive de la communauté d'invertébrés avec le taux de dénitrification a été seulement visible pendant automne. Cette période est considérée comme un moment propice pour l'observation de la relation diversité-fonction dans ce milieu. / This PhD study aims to understand how the biodiversity influences the water purification processes in the hyporheic zone of running water, as an important regulating service that reduces the quantity of pollutants in freshwater ecosystems. Few studies have focused on how the invertebrate community influences the functioning of hyporheic zones, which are considered as a biogeochemical reactor that largely contributes to nutrient cycling capacity of the rivers. Nitrate retention or denitrification functions in hyporheic zones are used as indicators for the water purification service. The relationship between the nitrate removal function and its associated biodiversity was tested at different scales from indoor microcosms to in-stream reaches and the hyporheic habitat of a large river (Garonne) meander, under natural and stressful conditions. First, the linkage between invertebrates and the nitrate (NO3-) removal function was given in evidence in a series of infiltration columns that mimicked the riverbed conditions with its benthic communities. A gradient of community diversity was created with biofilm, meiofauna and macrofauna communities' combination in different treatments. It enabled to test the influence of the invertebrate community on the NO3- removal rates with and without pesticides during the Inbioprocess project. The results implied the influence of invertebrate and microbial cross-community interactions on NO3- removal rates, which was higher with invertebrate communities in the sediments (11.8 ± 1.2) than without (7.7 ± 1.4 mg N.l-1.d-1). These findings suggested a top-down control of invertebrates on the microbial activities. These interactions were also depicted at the source of the recovery of the NO3- removal capacity when facing stressful conditions due to addition of pesticide in the experimental water. These laboratory findings highlighted the importance of multi-trophic level interactions in the hyporheic habitat, with probable implication of the more resistant species in the resilience capacity of this system. The occurrence of the top-down linkage was then explored in in situ habitats. The NO3- removal rates measured at the reach scale in 9 European streams during the STREAMES project ranged from 0.04 to 10.75 with an average of 1.64 ± 2.39 mg NO3--N m-2.min-1 (Mean ± SE). The results suggested that not only physico-chemical and hydrological factors, but also macro-invertebrate assemblages may influence nitrate removal. Some functional groups positively correlated with nitrate reduction were biofilm grazers and interstitial organisms associated with macro-porous substrate. In the hyporheic water of Monbequi meander of the Garonne river, the positive correlation between invertebrate diversity and the potential denitrification rates was only visible during the autumn season, suggesting a potential "hot moment" for the observation of this correlation between biodiversity and ecosystem function in fields.

Les activités anthropiques affectent les cycles biogéochimiques globaux, principalement par l'ajout de nutriments dans les écosystèmes. Il est donc crucial de déterminer dans quelle mesure les cycles biogéochimiques globaux peuvent être régulés. Les autotrophes peuvent réguler les réservoirs de nutriments par la consommation des ressources, mais la majorité des ressources leur sont inaccessibles à l'échelle globale. Par des modèles théoriques, nous avons cherché à évaluer la manière dont les autotrophes répondent à la fertilisation à l'échelle globale et leur capacité à réguler les concentrations des nutriments quand leur accessibilité est limitée. Nous avons également étudié les mécanismes qui déterminent la régulation des rapports de Redfield dans l'océan, ainsi que les effets de l'ajout de nutriments sur la production primaire océanique totale. Nous avons montré que les organismes ne régulent pas efficacement les réservoirs de nutriments. Le couplage des cycles biogéochimiques et la compétition entre groupes fonctionnels peuvent altérer, négativement ou positivement, la régulation des cycles biogéochimiques globaux par les organismes. Une régulation inefficace des concentrations de nutriments n'exclut par contre pas une forte régulation des rapports entre ces nutriments, comme dans le cas des rapports de Redfield. La fertilisation des écosystèmes terrestres et océaniques risque donc de fortement impacter la production primaire et les cycles biogéochimiques globaux, à de courtes comme à de grandes échelles de temps. / Anthropogenic activities heavily impact global biogeochemical cycles, mainly through nutrient fertilisation of ecosystems; thus it is crucial to assess the extent to which global biogeochemical cycles are regulated. Autotrophs can regulate nutrient pools locally through resource consumption, but most resources are inaccessible to them at global scales. We used theoretical models to assess how organisms respond nutrient fertilisation at global scales and how they can regulate the concentration of these nutrients when their accessibility of is limited. We further investigated the mechanisms driving the regulation of Redfield ratios in oceans, and the effects of nutrient fertilisation on total oceanic primary production. We showed that organisms cannot efficiently regulate nutrient pools. Mechanisms such as coupling of nutrient cycles and competition between functional groups can alter the strength of biotic regulation of global biogeochemical cycles, either positively or negatively. An inefficient regulation of inaccessible nutrient concentration, however, does not exclude a strong biotic regulation of nutrient ratios, as is the case with Redfield ratios in oceans. Nutrient fertilization of oceanic and terrestrial ecosystems is thus likely to have a strong impact on primary production and global nutrient cycles at both small and long timescales.

Cette thèse a pour but de comprendre les implications des relations entre réseau trophique vert et réseau trophique brun sur la stabilité et le fonctionnement des réseaux trophiques. Les interactions entre ces deux réseaux, respectivement fondés sur la photosynthèse et la consommation de la matière organique morte, sont essentielles au fonctionnement des écosystèmes : l'un produit de la matière organique à partir de nutriments minéraux et l'autre recycle les nutriments contenus dans la matière organique morte. Cette question est abordée à l'aide de deux modèles théoriques et d'une étude expérimentale. Mon premier modèle montre que la boucle de rétroaction induite par le recyclage des nutriments dans un réseau trophique exclusivement vert a un effet stabilisant sur les dynamiques d'une chaîne trophique et un effet enrichissement à cause de la remise à disposition pour les producteurs primaires des nutriments excrétés par l'ensemble des organismes du réseau trophique. Cependant seul l'effet enrichissement, qui est déstabilisant, persiste dans un modèle de réseau trophique. Mon second modèle intègre le réseau brun de manière explicite et montre que ce réseau est davantage déstabilisé que le réseau vert lorsque la disponibilité en nutriments augmente. Cette effet est amplifié si la majeure partie de l'excrétion se fait sous forme de détritus qui déstabilisent le réseau brun par un effet d'enrichissement. Ce modèle montre également que la survie des consommateurs est améliorée lorsqu'ils peuvent consommer des proies provenant des deux réseaux. Mon expérience en mésocosmes aquatiques a permis d'étudier les effets en cascades entre réseaux vert et brun via une filtration de la lumière (manipulation directe du réseau vert), l'ajout de carbone organique dissous (manipulation directe du réseau brun) et l'ajout de poissons (manipulation de la structure du réseau trophique). Nous n'avons pas observé d'effets en cascade du réseau vert sur le réseau brun et inversement, notamment à cause d'un ajout probablement trop faible de carbone dissout. Les poissons ont eux eu un fort effet sur les deux réseaux avec des effets positifs sur le phytoplancton lorsque la lumière est réduite à cause de la diminution de la limitation par les nutriments grâce à l'excrétion des poissons, une augmentation de la concentration en carbone organique dissout et une modification du profil métabolique de la communauté bactérienne benthique. Un modèle annexe montre quant à lui que la plasticité du métabolisme chez les organismes, c'est-à-dire leur capacité à réduire ou à augmenter leur métabolisme en fonction de la disponibilité en ressources afin de maximiser leur bilan énergétique permet de stabiliser les dynamiques d'une chaine trophique en diminuant la variabilité temporelle des biomasses des espèces. Dans un réseau trophique, cette stabilisation se traduit par une augmentation de la persistance des espèces. Cette thèse a permis de mieux relier l'écologie des communautés à l'écologie fonctionnelle, améliorant ainsi notre compréhension des conséquences de grands processus écosystémiques comme le recyclage des nutriments sur la stabilité des réseaux trophiques et des effets de la structure de ces réseaux sur le fonctionnement des écosystèmes. / The aim of this thesis is to understand the implications of the relationships between green and brown food webs on the stability and functioning of food webs. The interactions between these two food webs, based respectively on photosynthesis and the consumption of dead organic matter, are essential for the functioning of ecosystems: one produces organic matter from mineral nutrients and the other one recycles the nutrients contained in dead organic matter. I address this by using two theoretical models and an experimental study. My first model shows that the feedback loop induced by nutrient cycling in an exclusively green food web has a stabilising effect on species dynamics in a food chain and an enrichment effect due to the excretion of nutrients that are available again for primary producers. However, only the enrichment effect, which is destabilising, persists in a food web model. My second model integrates a true brown food web and shows that this food web is more destabilised than the green food web when nutrient availability increases. This effect is amplified if most of nutrients are excreted as detritus that destabilises the brown food web through an enrichment effect. This model also shows that consumer survival is improved when they can consume prey from both green and brown food webs. My experiment in aquatic mesocosms enabled me to study the cascading effects between green and brown food webs thanks to light filtration (direct manipulation of the green food web), the addition of dissolved organic carbon (direct manipulation of the brown food web) and the addition of fish (manipulation of food web structure). We did not observe any cascading effects of the green food web on the brown food web and vice versa, probably because of a too low addition of dissolved carbon. The fish had a strong effect on both green and brown food webs with positive effects on phytoplankton when light is filtered because of the decreased nutrient limitation thanks to fish excretion, an increased concentration of dissolved organic carbon and a change in the metabolic profile of the benthic bacterial community. An additional model shows that the plasticity of metabolic rate, that is the ability of organisms to increase or decrease their metabolic rate depending on resource availability in order to optimise their energy budget, stabilises species dynamics in a food chain model by decreasing biomass time variability. Such a stabilising effect results in increase of species persistence in a complex food web model. This thesis is an additional step to better link community ecology to functional ecology, thus improving our understanding of the consequences on food web stability of major ecosystem processes such as the nutrient cycling and the effects of food web structure on ecosystem functioning.