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Breast cancer is the most common malignancy and a major cause of cancer-related death among Canadian women. Although treatment of primary breast tumours is highly successful through surgery, metastatic breast cancer is difficult to treat. Cancer progression and metastasis require the accumulation of numerous genetic and epigenetic alterations. Normal cells that acquire such alterations can transform into cancer cells, resulting in primary tumour formation. Primary tumours are a heterogeneous population, containing cells of various metastatic potentials. Cells that acquire a high potential for metastasis can spread to secondary locations. Our model system consists of two subpopulations, with different metastatic potential, derived from the same rat mammary adenocarcinoma. Using this model, a differentially expressed novel gene, termed MS-1, was discovered. Due to significant expression of this gene in the poorly metastatic subpopulation and lack of expression in the highly metastatic subpopulation, MS-1 may have involvement in metastasis suppression. Several breast cancer metastasis suppressor genes have been identified on the basis that they are down-regulated during the progression of metastasis. Epigenetic mechanisms, such as DNA methylation, account for loss of expression in several of these genes. Hypermethylation of CpG islands within gene promoters results in deacetylation of histone proteins and produces a compact chromatin structure that is unfavourable for transcription. A CpG island spans the 5 untranslated region, exon 1 and part of intron 1 of the MS-1 gene. Our data reveals aberrant methylation patterns of this CpG island in our model. Also, MS-1 expression appears to be partially induced by both DNA methylation and histone deacetylation inhibitors. Following a screen of several cancer cell lines of various metastatic potential, it appears that the presence of DNA methylation in the CpG island of MS-1 correlates with the lack of MS-1 expression. Therefore, these results suggest that MS-1 may be silenced in cells of high metastatic potential through epigenetic mechanisms.

Breast cancer is the most common malignancy and a major cause of cancer-related death among Canadian women. Although treatment of primary breast tumours is highly successful through surgery, metastatic breast cancer is difficult to treat. Cancer progression and metastasis require the accumulation of numerous genetic and epigenetic alterations. Normal cells that acquire such alterations can transform into cancer cells, resulting in primary tumour formation. Primary tumours are a heterogeneous population, containing cells of various metastatic potentials. Cells that acquire a high potential for metastasis can spread to secondary locations. Our model system consists of two subpopulations, with different metastatic potential, derived from the same rat mammary adenocarcinoma. Using this model, a differentially expressed novel gene, termed MS-1, was discovered. Due to significant expression of this gene in the poorly metastatic subpopulation and lack of expression in the highly metastatic subpopulation, MS-1 may have involvement in metastasis suppression. Several breast cancer metastasis suppressor genes have been identified on the basis that they are down-regulated during the progression of metastasis. Epigenetic mechanisms, such as DNA methylation, account for loss of expression in several of these genes. Hypermethylation of CpG islands within gene promoters results in deacetylation of histone proteins and produces a compact chromatin structure that is unfavourable for transcription. A CpG island spans the 5 untranslated region, exon 1 and part of intron 1 of the MS-1 gene. Our data reveals aberrant methylation patterns of this CpG island in our model. Also, MS-1 expression appears to be partially induced by both DNA methylation and histone deacetylation inhibitors. Following a screen of several cancer cell lines of various metastatic potential, it appears that the presence of DNA methylation in the CpG island of MS-1 correlates with the lack of MS-1 expression. Therefore, these results suggest that MS-1 may be silenced in cells of high metastatic potential through epigenetic mechanisms.

Thesis (PhD)--Macquarie University, Division of Humanities, Dept. of Ancient History, 2000. / Bibliography: p. 475-498. / PART I -- Thesis introduction -- Location, environment and routes of the Western Desert -- Cultural, historical and archaeological setting of Dakhleh Oasis -- Introduction to the vessel typology -- Introduction to the site catalogue -- Technology of pottery manufacture -- Fabrics and wares -- Conclusion -- PART II -- The vessel typology -- The site catalogue. / This thesis analyses a body of largely unpublished ceramic material from Dakhleh Oasis in the Western Desert of Egypt. The material is primarily from the survey of Dakhleh Oasis and the testing of sites by members of the Dakhleh Oasis Project and, except for some Phase 4 material recovered from excavations at Ismant el-Kharab, is unstratified. It covers a thousand years of Egyptian pottery-making from the eighth century BC to the late second century AD. -- A comprehensive survey of published and unpublished material from other sites in Egypt and adjacent regions has been undertaken to acquire comparative material for the pottery from Dakhleh Oasis. In addition, a study of the technical characteristics of the vessels that have remained accessible has been undertaken to describe and explain ancient pottery practices and to build up a framework for comparative purposes. -- With this body of information, a vessel typology divided into two series, each of which are further divided into two phases, has been devised and the chronology of the vessels determined. This ceramic typology has been used to compare surveyed sites of different utilisation - cemetery, settlement and temple sites - and to establish a dating system for these sites. The resulting chronology will be a guide to the determination of future excavations in the oasis and will assist in the on-going study of the socio-economic development of the oasis. The typology also provides a corpus of pottery for the processing of material from future excavations in Dakhleh Oasis and information for other ceramicists working in Egypt and elsewhere. -- The comparative survey of ceramic material from other sites demonstrates that Dakhleh Oasis, although a remote region in the Western Desert of Egypt, maintained contact with the Nile Valley and more distant areas. It also shows that, while this interaction influenced local pottery styles, the oasis retained and developed its own pottery traditions. -- In addition, a preliminary analysis has been made of fabrics and clays for descriptive purposes and to increase knowledge of the ancient ceramics from the oasis. -- A database has also been built to store and manipulate the information on this extensive body of ceramic material from Dakhleh Oasis. The pottery drawings have been produced in a format readily accessible for electronic transfer to researchers in the field of Egyptian ceramics. / Mode of access: World Wide Web. / 498, [199] p. ill. (some col.), maps

The eXtensible Access Control Markup Language (XACML) is allowing enterprises to implement a standard way of access control to their resources. Security administrators no longer need to duplicate their efforts in writing multiple policies for different resources since a XACML policy can be applied to multiple resources. Companies such as IBM, Sun Microsystems, Oracle, and Cisco are already providing integration of XACML in their products. Although the introduction of XACML has provided enterprises with a better approach of commonly realizing access control, there are still inconsistencies that policy administrators need to be aware of. This thesis identifies some of the inconsistencies in XACML today and introduces a new tool that can be used to visualize some of those inconsistencies. This new tool could be used by a policy administrator to visualize possible conflicting data among a set of policies.

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Thesis (Ph.D.) -- McMaster University, 1999. / Includes bibliographical references (leaves 303-319). Also available via World Wide Web.

Histology is a technique that has any number of diagnostic uses in modern hospital laboratories. However, as a scientific method employed in the study of ancient and mummified remains, it appears to have lost its popularity.This project explores the advantages and limitations of histology as a technique for such studies. In order to do so, soft tissue and bone samples from ten early Roman Period mummies (30 BCE – 250 CE) from the Kellis 1 cemetery in the Dakhleh Oasis have been histologically examined.While this project focuses on the scientific technique of histology, and its application for the study of ancient remains, it also aims to be cross-disciplinary by incorporating scientific results from the ten mummies with the historical data and archaeological remains uncovered during excavations of the Kellis site. By bringing the results of science and Egyptology/archaeology together, it hoped that a better understanding of ancient Egyptian society could be achieved.