Inflammatory processes mark all stages of atherogenesis. One of the key regulators of inflammation is the transcription factor nuclear factor kappa B (Nf-kb). Nf-kb is the general name for a whole family of dimeric transcription factors. One can distinguish between a classical and an alternative pathway with Rela/p50 (Nf-kb1) and Relb/p52 (Nf-kb2) representing the terminal transcription factors, respectively. Classical Nf-kb1 signaling has been associated with atherosclerotic lesion development many times, mainly because of its regulation of many pro-inflammatory proteins with an established role in atherogenesis. Recent studies provided evidence of crosstalk between classical Nf-kb1 and alternative Nf-kb2 signaling, implicating a potential role for Nf-kb2 in atherogenesis. The aim of the present study was to investigate the influence of Nf-kb2 on atherosclerotic lesion development in a knockout mouse model. Nfkb2 knockout (Nfkb2-/-) mice were generated on two different atherosclerosis sensible backgrounds, the Apoe- and Ldlr- deficient background. Quantification of atherosclerotic lesion development showed, that Nfkb2-/- mice developed significantly more atherosclerosis at the brachiocephalic artery than wild type controls, indicating a protective effect of Nf-kb2 on atherogenesis. Further expression analyses in bone marrow-derived macrophages (BMDM) revealed highly significant upregulation of matrix metalloproteinase 9 (Mmp9) in Nfkb2-/- mice. Overexpression of Mmp9 was associated with enhanced macrophage migration across extracellular matrix in vitro and an inflammatory plaque phenotype with advanced, macrophage-rich lesions. Accordingly, increased Mmp9 expression in Nfkb2-/- macrophages might have contributed to enhanced lesion development in these mice.

This thesis is an autoethnography exploring the engagement of records management (RM) through the vehicle of a computer mediated communication (CMC) focused co-operative inquiry. CMC is defined as, “communication that takes place between human beings via the instrumentality of computers” (Herring, 1996, p.81). The PhD stance was that with the advent of new technologies, such as CMC, the role and place of RM has been challenged. RM practitioners needed to evaluate their principles and practice in order to discover why RM is not uniformly understood and also why it fails to engage many CMC users and information professionals. The majority of today’s information is generated as the result of unstructured communications (AIIM, 2005 and 2006) that no longer have a fixed reality but exist across fragmented globalised spaces through the Cloud, Web 2.0 and software virtualisation. Organisational boundaries are permanently perforated and the division between public and private spaces are blurred. Traditional RM has evolved in highly structured organisational information environments. Nevertheless, RM could lie at the heart of the processes required for dealing with this splintered data. RM takes a holistic approach to information management, establishing the legislative requirements, technical requirements and the training and support for individuals to communicate effectively, simultaneously transmitting and processing the communications for maximum current and ongoing organisational benefits. However RM is not uniformly understood or practiced. The focus of the thesis was to understand how RM engagement can and should be achieved. The research was conducted by establishing a co-operative inquiry consisting of 82 international co-researchers, from a range of disciplines, investigating the question, ‘How do organisations maximise the information potential of CMC for organisational benefit, taking into account the impact of the individual?” The PhD established a novel approach to co-operative inquiry by separating, managing and merging three groups of co-researchers (UK Records Managers, UK CMC users, international Records Managers and CMC users). I was embedded as a co-researcher within this wider inquiry personally exploring as an autoethnography the relevance of RM to the wider research question, the ability of RM practitioners to advocate for RM and the co-researchers’ responses to the place of RM within this context. The thesis makes several contributions to the research field. It examines how records managers and RM principles and practice engaged through the inquiry, articulating the reasons why users sometimes failed to engage with RM principles and practice, and what assists users to successfully engage with RM. It was found that national perspectives and drivers were more significant as to whether or not individuals engaged with RM concepts than age, gender or professional experience. In addition, users engaged with RM when it was naturally embedded within processes. In addition, as a result of the inquiry’s discussions and actions, the thesis suggests that RM principles and practice need to be refined, for example in regards to the characteristics that define a record. In this respect it concludes that there is rarely likely to be an original archival record surviving through time given the need for migration. The research delivered a novel approach to co-operative inquiry whereby merging groups through time produced new learning at each merger point. The thesis recommends further research to build upon its findings.

The seed germination process during sprout production provides suitable environmental conditions for the growth of pathogenic bacteria, such as Listeria monocytogenes. A potential way to control this bacterial growth is through the use of bacteriophages, which are naturally occurring viruses that specifically attack bacterial targets and have been shown to be effective antimicrobials in some foods. Therefore, the objective of this study was to evaluate the antimicrobial susceptibility of L. monocytogenes to bacteriophage on alfalfa sprouts during seed germination and subsequent refrigerated storage at 4 °C. Alfalfa sprout seeds were dip-inoculated with 5.5 x 105 CFU/ml L. monocytogenes serogroups 1 and 4. This was followed by treatment with the commercial bacteriophage LISTEX™ P100 at a concentration of 5.3 x 107 PFU/ml. The seeds were then soaked and germinated for 80 h using the glass jar method. The concentration of L. monocytogenes was determined every 24 h using PALCAM agar plated in triplicate. When compared to the spiked, untreated control, treatment of sprout seeds with LISTEX™ P100 resulted in a statistically significant (p < 0.05) reduction of 1.6 log10 CFU/g L. monocytogenes after the initial 24 h of germination. However, the bacteriophage did not show a lasting inhibitory effect, with no statistically significant reductions in L. monocytogenes growth as compared to the control at subsequent time points. The bacteriophage remained stable over the entire germination and storage period. Although biocontrol of Listeria with bacteriophages has high potential to serve as an alternative strategy to control foodborne illnesses, factors such as phage delivery and dose optimization in sprouts need to be further investigated.

Thesis (MSc (Biochemistry))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The ostrich industry in South Africa is currently threatened by respiratory disease in feedlot ostriches which causes a dramatic loss in production. Ms01, Ms02 and Ms03 were identified as the three ostrichspecific mycoplasmas to be associated with this respiratory disease in ostriches of South Africa. The ostrich-specific mycoplasmas have a major impact on ostrich production and for this reason there is a serious need for treatment for these infections. For this reason, the ostrich industry has undertaken an investigation into the development of vaccines against mycoplasma infections. In this study, an approach to DNA vaccine development will be investigated and applied, specifically for the ostrich mycoplasma Ms02. Firstly, the whole genome of Ms02 was sequenced using GS FLX sequencing technology. The contiguous sequences obtained from the whole-genome sequencing were analysed bioinformatically which included the annotation of the contiguous sequences and the subsequent search for a vaccine candidate gene for the development of a DNA vaccine. The P100 gene of Ms02, which showed a high degree of homology with the P100 gene of the human pathogen M. hominis, was chosen as a vaccine candidate gene for the development of a DNA vaccine. The P100 gene was successfully cloned and subsequently modified by means of site-directed mutagenesis to correct for alternative codon usage, where after the modified P100 gene was subcloned into the mammalian expression vector, pCI-neo for vaccination trials in the near future. / AFRIKAANSE OPSOMMING: Die volstruisbedryf van Suid-Afrika is tans bedreig deur 'n respiratoriese siekte in voerkraal volstruise wat lei tot aansienlike verliese in volstruisproduksie. Ms01, Ms02 en Ms03 is geïdentifiseer as die drie volstruis-spesifieke mikoplasmas wat 'n rol speel in hierdie respiratoriese siektes van volstruise in Suid- Afrika. Die drie volstruis-spesifieke mikoplasmas het 'n groot impak op die produksie van volstruise en om hierdie rede is daar 'n ernstige behoefte aan 'n behandeling van hierdie infeksies. Ten einde mikoplasma infeksies in volstruise te voorkom, het die Suid-Afrikaanse volstruisbedryf 'n ondersoek geloods na moontlike strategieë vir entstof ontwikkeling. In hierdie studie, is 'n benadering van DNA entstof ontwikkeling ondersoek en toegepas, spesifiek teen die volstruis mikoplasma Ms02. Eerstens, is die volledige Ms02 genoomvolgorde bepaal deur gebruik te maak van GS FLX volgordebepalingstegnologie. Die gedeeltelike volgordes verkry vanaf die heelgenoom volgordebepaling is bioinformaties geanaliseer wat die annotering van die gedeeltelike volgordes asook die soektog vir 'n kandidaat entstof geen vir die ontwikkeling van 'n DNA entstof ingesluit het. Die P100 geen van Ms02, wat hoë homologie met die P100 geen van die menslike patogeen M. hominis getoon het, is gekies as die kandidaat entstof geen. Die P100 geen is suksesvol gekloneer en gemodifiseer deur middel van setelgerigte mutagenese om die P100 geen geskik te maak vir die invoeging in die soogdier ekspressie vektor, pCI-neo vir toekomstige entstofproewe.

The purpose of this research is to assess the function, form and content of knowledge sharing in communities of practice, social networks and the use of collaborative technologies in Third Sector community networks in the North East of England. This is a significant area worthy of detailed examination due to the acknowledged relationship between communities of practice, social networks and the use of collaborative technologies. These three domains have been examined separately by others and suggestions have been made as to relationships between them but few, if any, studies appear to have used case-based evidence to explore how these relationships add value to knowledge sharing. The research addresses the following research question: To what extent does the use of collaborative technologies in communities of practice and social networks, in the Third Sector of the North East region, add value to face- to-face knowledge sharing and how may this be measured? In order to answer the research question a qualitative holistic case study approach based upon three case studies in Newcastle upon Tyne, South Tyneside and Sunderland has been utilised and grounded theory is used to formulate theory from the observed and analysed practice of the case studies under investigation. The conclusion is drawn that when value is added to knowledge sharing it is relative to the strength of several key variables, including, reciprocity, trust, the strength of network ties and the ability to integrate the use of collaborative technologies into ongoing activities. To aid analysis of the presence and strength of these variables a working paradigm has been designed and developed. Case studies are analysed through this paradigm leading to the development of a theory of knowledge sharing in the Third Sector.

Promoting knowledge sharing has long been regarded as a very important aspect of the management of knowledge. However, knowledge sharing has its challenges due to the special nature of knowledge. Based on this, the researcher argues that it is knowledge seeking rather than knowledge sharing that plays a crucial role in knowledge management. However, there is no clear definition for knowledge seeking in existing literature. In the few studies of knowledge seeking research, knowledge has been viewed as a noun and as such knowledge seeking has been seen as no different to information seeking. The aim of this research has been to explore the knowledge seeking process in the workplace in order to conceptualise knowledge seeking by developing a theoretical model. A review of the literature concerning knowledge seeking has been conducted in order to clarify the concept of knowledge seeking. From the interpretivist’s perspective, a qualitative research approach has been taken, in which sense-making theory is employed as a methodological guide. Time-line interviews were carried out with construction engineers in China to collect primary data, and Template analysis was utilized. Based on the literature, this thesis defined knowledge seeking as a learning process, which consists of three major themes: experiential learning, information seeking and problem solving, based on which a preliminary framework was developed. Twenty six engineers were successfully interviewed. The findings from the data confirmed the links between the themes. Further codes were also identified to develop a final template, which evolved to a theoretical model illustrating the knowledge seeking process in the workplace. By promoting knowledge seeking rather than knowledge sharing, this research contributed innovatory insight into existing KM research. The new concept of knowledge seeking and the theoretical model developed thereafter contribute to knowledge by providing a theoretical framework for further research in this area. The specific combination of time-line interviews and template analysis has demonstrated good results in this research. Collecting primary data from China, this research applied Western theories onto engineers within a Chinese context, which has contributed to KM research in China. These contributions will result in many practical implications for KM practices.

This doctoral study sets out to investigate how Key Stage 2, primary school children (aged between 7 and 11 years) are being taught to develop information seeking skills and strategies that will allow them to become critical and literate users of information. The methodological approach adopted for this research study is that of interpretivist ethnography. This represents a move away from the traditional quantifiable approaches used in Library and Information Science (LIS) and focuses on gathering data in the natural setting in order to offer a rich picture of the information seeking behaviour of a small group of Key Stage 2 children. In order to become an integrated part of the natural setting it was necessary to become immersed in the school for an extended period of time, one academic school year. Taking a grounded theory approach meant that it was possible, right at the beginning of the fieldwork, to identify situations as they occurred. During the field research, a rich picture emerged of the information seeking strategies and skills of the group of children participating in the study. As the field research progressed, themes and patterns were identified which were then examined against previous research in order to identify similarities or differences in the findings of this study with other studies. The themes that unfolded from the data offered an information seeking model that was specific to the group of Key Stage 2 children. Within the model a further theoretical framework is offered that postulates that a zone of optimal learning exists. The theory suggests that there is an optimal cognitive zone that is the place where new information is assimilated so that it can become knowledge. The zone of optimal learning is the place where a child moves from incomprehension of new information to a cognitive understanding of that information. Investigatingth e influencesu pont he children'si nformations eekingb ehaviour served to highlight both strengths and weaknesses in the ways in which the children developed their information seeking skills and strategies and with this in mind a set of four suggestions are offered that aim to support the way in which information seeking strategies are delivered to Key Stage 2 children based on the children that participated in this study.

This research aims to investigate current volunteer use in public libraries in England. Volunteer use is not a new phenomenon, and has been an integral part of public library provision for many years. However recent Government policies, together with greater financial austerity, have resulted in a change in public service delivery. There has been a dramatic increase in the number of volunteers involved within the public library service, and the growth of community run libraries, resulting in much public and professional concern. An interpretivist research approach was used to investigate stakeholder opinions regarding volunteer use, and involved a two phase process. Initially a Delphi survey explored attitudes of 15 English public library service managers, followed by in-depth investigation of two case study library authorities, located in the North-East of England. Surveys, interviews and focus groups, helped to build a rich picture of volunteer use amongst the groups of stakeholders. Findings clearly indicated that volunteer use has moved from additionality to replacement of staff, and is increasingly being used by local authorities as a solution to budget reductions required as a result of economic austerity. A hybrid approach to library service provision has developed, using a combination of paid staff and volunteers, which indicates a fundamental culture shift within public libraries. Research results identified concerns relating to the long term viability of a hybrid approach, and how this impacted on the wider community in terms of service provision. Key concerns were raised concerning advocacy, sensitivity, the fragility of relationships, and the provision of an accountable and high quality service. Formal and informal control mechanisms need to be employed by library service managers to ensure that they reap the benefits of volunteer use, thereby avoiding social exclusion, clarifying stakeholder boundaries, and delivering a high quality accountable service. Training library managers in new volunteer management skills, and adopting a volunteer relationship management approach may help to ensure that this new arrangement is mutually beneficial for all concerned.

No description available.

Zusammenhänge zwischen neurokognitiven Indikatoren und Verhaltensindikatoren der Gesichterkognition können Gehirnsysteme und neuronale Subprozesse identifizieren, die individuellen Unterschieden im Verhalten zugrunde liegen. Diese Dissertation zeigt, dass Ereigniskorrelierte Potentiale (EKPs) als neurokognitive Indikatoren für die Erforschung individueller Unterschiede eingesetzt werden können, denn sie weisen die gleichen hohen psychometrischen Qualitäten wie andere Fähigkeitsindikatoren auf und messen daher individuelle Unterschiede in der neuronalen Verarbeitung zuverlässig und stabil über die Zeit. Auf der Verhaltensebene wurden drei Teilfähigkeiten der Gesichterkognition etabliert: Gesichterwahrnehmung, Gesichtergedächtnis und Gesichtergeschwindigkeit. EKPs wurden in Strukturgleichungsmodellen verwendet, um den Beitrag neurokognitiver Indikatoren an individuellen Unterschieden dieser Gesichterkognitionsfähigkeiten zu schätzen. Für 85 Probanden wurden Beziehungen zwischen den Gesichterkognitionsfähigkeiten und der P100, N170, der sogenannten Differenz aufgrund des Gedächtnisses (Dm) und dem frühen sowie späten Wiederholungseffekt (ERE und LRE) etabliert. Spezifische Anteile individueller Unterschiede in der Gesichterkognition auf der Verhaltensebene wurden durch individuelle Unterschiede im Zeitverlauf der strukturellen Gesichteranalyse (N170 Latenz) sowie in der Reaktivierung von Repräsentationen gespeicherter Gesichtsstrukturen (ERE) als auch personen-spezifischen Wissens (LRE) erklärt. Keinen Anteil an individuellen Unterschieden erklärten hingegen frühe Wahrnehmungsprozesse (P100), die neuronale Aktivierung während der strukturellen Gesichteranalyse (N170 Amplitude) und Prozesse der Gedächtnisenkodierung von Gesichtern (Dm). Diese Ergebnisse zeigen, dass individuelle Unterschiede in der Gesichterkognition von der strukturellen Gesichteranalyse sowie von der Effizienz und Geschwindigkeit des Zugriffs auf Gedächtnisinhalte zu Gesichtern und Personen abhängt. / Individual differences in perceiving, learning, and recognizing faces were shown on the behavioral and neural level but were rarely related to one another. By determining relationships between behavioral and neurocognitive indicators of face cognition, brain systems and neural sub-processes can be identified that underlie individual variations on the behavioral level. The present dissertation laid the foundation for using event-related potentials (ERPs) as neurocognitive indicators in individual differences research. ERP components were shown to possess the same high psychometric qualities as behavioral ability measures and thus to measure individual differences of neural processing reliably and stably across time. On the behavioral level, three component abilities of face cognition were established: face perception, face memory, and the speed of face cognition. ERP components were used in structural equation models that estimated contributions of neurocognitive indicators to the individual differences in these face cognition abilities. Regression analysis was used to determine the contributions of P100, N170, the so called difference due to memory (Dm), as well as early and late repetition effects (ERE and LRE) to face cognition abilities in 85 participants. Certain amounts of variance in face cognition as seen on the behavioral level were accounted for by individual differences in the temporal dimension of structural encoding of a face (N170 latency) and in the re-activation of both stored facial structures (ERE) and person-identity information (LRE). In contrast, processes of early vision (P100), the neural activation of structural face encoding (N170 amplitude), and memory encoding of new faces (Dm) did not show any contribution to individual differences in face cognition. These findings show that individual differences in face cognition depend on the speed of structurally encoding faces and on the efficiency and speed of accessing face and person memory.