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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
11

The mechanisms of Pol expression and assembly for human foamy virus /

Baldwin, David Norris. January 1999 (has links)
Thesis (Ph. D.)--University of Washington, 1999. / Vita. Includes bibliographical references (leaves 98-107).
12

Os desafios das pol??ticas sociais na contemporaneidade: o caso da Prefeitura de Belo Horizonte

Gomes, Ana Paula Salej January 2007 (has links)
Submitted by Roger Guedes (roger.guedes@fjp.mg.gov.br) on 2015-08-17T20:52:42Z No. of bitstreams: 1 Os Desafios das Pol??ticas Sociais na Contemporaneidade.pdf: 2924550 bytes, checksum: 7551f70a2fca0dbd7be598bfe4f1f033 (MD5) / Approved for entry into archive by Roger Guedes (roger.guedes@fjp.mg.gov.br) on 2015-08-17T20:53:19Z (GMT) No. of bitstreams: 1 Os Desafios das Pol??ticas Sociais na Contemporaneidade.pdf: 2924550 bytes, checksum: 7551f70a2fca0dbd7be598bfe4f1f033 (MD5) / Made available in DSpace on 2015-08-17T20:53:19Z (GMT). No. of bitstreams: 1 Os Desafios das Pol??ticas Sociais na Contemporaneidade.pdf: 2924550 bytes, checksum: 7551f70a2fca0dbd7be598bfe4f1f033 (MD5) Previous issue date: 2007 / Funda????o Jo??o Pinheiro / Em seu constante processo de transforma????o o Estado se depara com o problema das desigualdades. O trabalho analisar?? as pol??ticas sociais sob a ??gide da justi??a social, com destaque para a dicotomia entre redistribui????o e reconhecimento. Apoiando-se na literatura sobre exclus??o e na proposta de constru????o de um paradigma bidimensional ele apresenta o caso das pol??ticas sociais de Belo Horizonte. Na an??lise deste caso, a partir da pesquisa documental e emp??rica dos programas sociais do munic??pio, toma como refer??ncia a l??gica fuzzy e usa o m??todo Grade of Membership-GoM, para agrupar os diversos programas a partir de sua percep????o de injusti??a social. Ao mostrar a import??ncia da integra????o das perspectivas da redistribui????o e do reconhecimento na formula????o e na implementa????o de pol??ticas sociais adequadas ??s demandas do nosso tempo, o estudo recupera outras dimens??es e categorias importantes na estrutura????o da agenda social, como por exemplo o territ??rio, os grupos sociais, a focaliza????o, a universaliza????o e a intersetorialidade. / In his constant transformation process, the State deals with the inequality problem. This work will analyze the social politics under the protection of social justice, highlithing a dichotomy between redistribution and acknowledgement. Based on literacy about exclusion and on the proposal of a constrution of a bidimentional paradigm, it shows the events of social politics in Belo Horizonte. On the analysis of this events, through documental and empirical research on social programs of the county, it is used as a reference the fuzzy logics and it is utilized the GoM formula to group the many programs trough the perception of social injustice. By showing the importance of integration of the perspectives of redistribution and acknowledgment of formulation and implementation of adequated social politics, this study recovers other dimentions and important categories on estruturation of a social agenda, such as the territory, social groups, universalization, and intersectoriality. / Governo e Pol??tica
13

S?ntese e caracteriza??o de copol?meros de Poli(NIsopropilacrilamida- Co-6-O-Viniladipoil-D-Glicose)

Celino, Karoline N?brega 15 September 2016 (has links)
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-20T19:31:23Z No. of bitstreams: 1 KarolineNobregaCelino_DISSERT.pdf: 3448363 bytes, checksum: cfa50cf91ac40ab42de7c9708504bc89 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-22T00:01:11Z (GMT) No. of bitstreams: 1 KarolineNobregaCelino_DISSERT.pdf: 3448363 bytes, checksum: cfa50cf91ac40ab42de7c9708504bc89 (MD5) / Made available in DSpace on 2017-03-22T00:01:11Z (GMT). No. of bitstreams: 1 KarolineNobregaCelino_DISSERT.pdf: 3448363 bytes, checksum: cfa50cf91ac40ab42de7c9708504bc89 (MD5) Previous issue date: 2016-09-15 / Neste trabalho, foi realizada e s?ntese e caracteriza??o de copol?meros a partir de N-isopropilacrilamida(Nipam) e 6-O-viniladipoil-D-glicose(ADG), utilizando as raz?es molares Nipam:ADG 1:9, 3:7, 1:1, 7:3 e 9:1. Tamb?m foram sintetizados e caracterizados os homopol?meros de Nipam e ADG, com intuito avaliar a influ?ncia da adi??o de ADG na cadeia. Os pol?meros foram submetidos ? caracteriza??o por espectroscopia na regi?o do infravermelho (FTIR), resson?ncia magn?tica nuclear de hidrog?nio (1H RMN), reometria e an?lise elementar (CHNO). Os copol?meros obtidos a partir de 30, 50 e 70 mol% de ADG na alimenta??o apresentaram insolubilidade em ?gua. Esse resultado foi associado ? forma??o de arranjos micelares compactados, com superf?cie predominantemente hidrof?lica e n?cleo predominantemente hidrof?bico. Nesses casos, a porcentagem de ADG incorporada, indicada por 1H RMN e CHNO, foi aproximadamente a mesma, o que justifica a diminui??o no rendimento dessas rea??es. Os espectros de FTIR e 1H RMN dos copol?meros comprovaram a inser??o da Nipam e do ADG em todas as amostras. Os copol?meros obtidos a partir de 10% e 90% mol de ADG na alimenta??o foram sol?veis em ?gua e sua composi??o foi determinada por 1H RMN, respectivamente, Nipam:ADG 9:1 e 3:7. O copol?mero Poli(Nipam-co-ADG) 9:1 promoveu um aumento significativo de viscosidade da ?gua, mesmo em baixas concentra??es, que pode ser atribu?do ao seu car?ter associativo. / In this study was performed synthesis and characterization of copolymers from N-isopropylacrylamide (Nipam) and 6-O-vinyladipoyl-D-glucose) (ADG) using the molar ratios of Nipam:ADG 1:9, 3:7, 1:1, 7:3 and 9:1. The homopolymers were also synthesized and characterized, Pnipam and poly(ADG), aiming to evaluate the influence of the addition of ADG in polymer. The polymers were submitted to characterization using spectroscopy in the infrared (FTIR), nuclear magnetic resonance of hydrogen (1H RMN), rheometry and elemental analysis (CHNO). The copolymers obtained from 30%, 50% and 70% mol of ADG feed had water insolubility. This result was associated with the formation of micellar arrangements compact with predominantly hydrophilic surface and core predominantly hydrophobic. In such cases, the percentage of embedded ADG, indicated by 1H NMR and CHNO, was about the same, which explains the decrease in the yield of these reactions. The FTIR and 1H NMR spectra of the copolymers confirmed the insertion of Nipam and the ADG for all samples. The copolymers obtained from 10% to 90% mol of the feed ADG were soluble in water and its composition was determined by 1 H NMR, respectively, Nipam:ADG 9:1 and 3:7. The poly(Nipam-co-ADG) 9:1 produced a significant increase of viscosity in water, even at low concentrations, which can be attributed to its associative character.
14

Turismo, pol?ticas p?blicas e desenvolvimento: uma avalia??o do programa de regionaliza??o do turismo nas cinco regi?es tur?sticas do Rio Grande do Norte (2004-2014)

Lima, Renata Mayara Moreira de 30 May 2017 (has links)
Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-08-10T11:45:50Z No. of bitstreams: 1 RenataMayaraMoreiraDeLima_TESE.pdf: 3934684 bytes, checksum: 0bfc7d75c547cdd6bb959f984a7970ce (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-08-11T11:23:25Z (GMT) No. of bitstreams: 1 RenataMayaraMoreiraDeLima_TESE.pdf: 3934684 bytes, checksum: 0bfc7d75c547cdd6bb959f984a7970ce (MD5) / Made available in DSpace on 2017-08-11T11:23:25Z (GMT). No. of bitstreams: 1 RenataMayaraMoreiraDeLima_TESE.pdf: 3934684 bytes, checksum: 0bfc7d75c547cdd6bb959f984a7970ce (MD5) Previous issue date: 2017-05-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / A import?ncia crescente do turismo, enquanto atividade econ?mica que dinamiza a economia de diversos lugares tem propiciado, cada vez mais, interven??es planejadas do Estado. No caso do Brasil, o turismo somente ocupa lugar de destaque no planejamento governamental com a cria??o do Minist?rio do Turismo (MTur) em 2003, momento que marca a concep??o do Programa de Regionaliza??o do Turismo (PRT), o qual tem sido concebido como estruturante na atual Pol?tica Nacional do setor. A constata??o da fr?gil literatura desenvolvida at? o momento sobre como avaliar pol?ticas p?blicas de turismo, bem como a necessidade de se mensurar com os mesmos crit?rios avaliativos os processos e resultados conseguidos com o PRT nas cinco regi?es tur?sticas do estado, nos motivaram a realizar a pesquisa ?Turismo, Pol?ticas P?blicas e Desenvolvimento: uma avalia??o do Programa de Regionaliza??o do Turismo nas cinco regi?es tur?sticas do Rio Grande do Norte (2004-2014)? que teve como objetivo geral avaliar a implementa??o do Programa de Regionaliza??o do Turismo no per?odo entre 2004 a 2014 no RN. Especificamente objetivou a) Analisar o desenho institucional do PRT; b) Compreender a conjuntura geogr?fica, econ?mica, sociocultural e de investimentos p?blicos realizados nos munic?pios de cada regi?o tur?stica do RN; c) Aferir os graus de efic?cia segundo os eixos de avalia??o e respectivos indicadores no ?mbito das regi?es e munic?pios escolhidos e d) Identificar os elementos facilitadores e dificultadores dos resultados do Programa, em ?mbito regional e municipal. O processo metodol?gico utilizado incluiu a elabora??o de uma matriz de avalia??o da implementa??o do Programa, al?m da revis?o bibliogr?fica, pesquisa documental, coleta de dados secund?rios e pesquisa de campo com visitas aos munic?pios e regi?es pesquisadas, assim como participa??o nas reuni?es dos conselhos de turismo e realiza??o de entrevistas. Os resultados apresentados confirmam a hip?tese defendida, de que o PRT apresenta no processo de sua implementa??o baixa efic?cia e resultados aqu?m das suas possibilidades nas regi?es tur?sticas do estado, em virtude da insufici?ncia dos recursos materiais, humanos e financeiros durante a implementa??o, bem como pela fragilidade com que ocorrem as articula??es institucionais necess?rias (nacional, estadual, regional e municipal). / The growing importance of tourism while an economic activity that invigorates the economy of several places has increasingly provided planned interventions by the State. In the case of Brazil, tourism is only a prominent part of government planning with the creation of the Ministry of Tourism (MTur) in 2003. This is the definitive moment when the Tourism Regionalization Program (PRT) is conceived as a structuring Current National Policy of the sector. The confirmation of the fragile literature developed so far on how to evaluate tourism public policies, as well as the need to measure the processes and results obtained with the PRT in the five tourist regions of the State, with the same evaluation criteria, motivated us to carry out the research "Tourism, Public Policies and Development: An evaluation of the Program of Regionalization of Tourism in the five tourist regions of Rio Grande do Norte (2004-2014)", whose overall objective was to evaluate the implementation of the Tourism Regionalization Program in the period 2004 to 2014 in RN. Specifically, it aimed at: a) Analyzing the institutional design of the PRT; b) Understand the geographical, economic, socio-cultural and public investment situation in the municipalities of each RN tourist region; c) Assess the degree of effectiveness according to the evaluation axes and respective indicators within the chosen regions and municipalities and d) Identify the elements that facilitate and impede the results of the Program at the regional and municipal levels. The methodological process used included the preparation of an evaluation matrix for the implementation of the Program, as well as bibliographical review, documentary research, secondary data collection and field research with visits to the cities and regions surveyed, participation in meetings of tourism councils and implementation of interviews. The results presented indicate that the PRT presents in the process of its implementation low effectiveness and results that are below its possibilities in the tourist regions of the State due to insufficient material, human and financial resources during its implementation, as well as the fragility with which the necessary institutional articulations are (be National, State, Regional and Municipal).
15

Nanoc?psulas formadas por dispers?o aquosa de poliuretano

Velho, Fernanda da Costa 03 March 2011 (has links)
Made available in DSpace on 2015-04-14T13:58:42Z (GMT). No. of bitstreams: 1 431128.pdf: 838056 bytes, checksum: 2f4ab7817bd95a0567306c4e09ec03dd (MD5) Previous issue date: 2011-03-03 / As nanopart?culas polim?ricas t?m sido desenvolvidas como uma importante estrat?gia para libera??o de f?rmacos convencionais, prote?nas, vacinas e, mais recentemente nucleot?deos. O estudo visando obter nanopart?culas polim?ricas com um sistema de libera??o programado e controlado ? de extrema relev?ncia. Este trabalho teve como objetivo a produ??o de nanoc?psulas de poliuretano-?leo (PU?leo) atrav?s de dispers?o aquosa de poliuretano, utilizando a policaprolactona (PCL) ou polietileno glicol (PEG) como poli?is, isoforona diisocianato (IPDI), ?cido dimetilol propi?nico (DMPA) como surfactante interno, lauril ?ter sulfato de s?dio (LESS) como surfactante externo e os ?leos de miglyol 812 e de a?a?. A s?ntese das nanoc?psulas foi realizada variando-se os par?metros reacionais como raz?o NCO/OH, concentra??o de surfactante externo e interno, concentra??o de ?leo, o tipo de ?leo e de poliol, a velocidade de agita??o e a etapa de dispers?o. A partir das nanoc?psulas formadas, foram feitos filmes e avaliados a degrada??o destes em meio tamponado, bem como, o pH das dispers?es, a rela??o de massa molecular entre pol?mero e ?leo, a solubilidade dos filmes obtidos frente ao ?leo de a?a? e, por fim, uma avalia??o preliminar do encapsulamento das nanoc?psulas. O m?todo de dispers?o aquosa de poliuretano se mostrou eficaz para a forma??o de nanoc?psulas possuindo como vantagem o uso de pequena ou nenhuma quantidade de solvente. Atrav?s das t?cnicas de MEV e MET foi poss?vel observar a forma??o de nanopart?culas de forma esf?rica com a presen?a de um n?cleo escuro envolto por uma membrana mais clara caracterizando uma nanoc?psula do tipo core-shell
16

S?ntese e caracteriza??o de poliamida termofund?vel

Freitas, Renata Fialho Rocha 06 July 2012 (has links)
Made available in DSpace on 2015-04-14T13:58:53Z (GMT). No. of bitstreams: 1 441029.pdf: 250230 bytes, checksum: 40a27c9638305e87ec4b7d6e314ec579 (MD5) Previous issue date: 2012-07-06 / Hot melt adhesives have the advantage that don t release volatile organic compounds as solvent-based adhesives. Polyamides containing dimer fatty acid in their structure can be used as hot melt adhesives. In this work polyamides with different compositions of dimer fatty acid, primary amines and secondary amines and their proportions were synthesized, the effect of these changes were evaluated in physical, chemical and mechanical properties. In relation to primary amines, ethylenediamine and hexamethylenediamine were used and it was found that a smaller chain gives an increase in the softening point, a higher elongation, lower Tg and a higher crystallization. The variation of secondary amines (DIPIP, piperazine, and aminoethylpiperazine) didn t affect the softening point. The piperazine based polyamide promotes better crystallization compared to the other structures of the amines (DIPIP and aminoethylpiperazine), because piperazine can promote higher organization of the chains. The methylenes groups from DIPIP and aminoethylpiperazine provide higher elongation of polyamides, increasing mobility of the chains. Increasing the content of ethylenediamine provides increased intermolecular interactions, giving a rise in the softening point, crystallization, viscosity and mechanical strength of the polyamide. Different compositions of dimer fatty acids were evaluated and was observed that the higher content of monomers, the lower molecular weight of the polyamide were achieved, consequently lower viscosity and more brittle polymers. Higher purity of dimer fatty acid promotes the formation of only one fraction of crystallization and melting. Performance bonding is usually associated by molecular weight of the polyamide and intermolecular interactions between substrate and polyamide chains. / Os adesivos termofund?veis apresentam a vantagem de n?o liberarem compostos org?nicos vol?teis (COVs), como no caso dos adesivos base solvente. Poliamidas contendo ?cido graxo dimerizado em sua estrutura podem ser utilizadas como adesivos termofund?veis. Neste trabalho foram sintetizadas poliamidas com diferentes composi??es de ?cido graxo dimerizado, aminas prim?rias e aminas secund?rias e suas propor??es e foi avaliado o efeito destas modifica??es nas propriedades f?sicas, qu?micas e mec?nicas. Em rela??o ?s aminas prim?rias, foi utilizado a etilenodiamina e hexametilenodiamina e verificou-se que uma cadeia menor confere um aumento no ponto de amolecimento, um maior alongamento, maior cristaliza??o e uma Tg menor. A varia??o das aminas secund?rias (DIPIP, piperazina e aminoetilpiperazina) n?o afetaram significativamente o ponto de amolecimento. A piperazina promoveu a poliamida uma maior cristaliza??o, comparado com as demais estruturas das aminas (DIPIP e aminoetilpiperazina), pois favorece uma maior organiza??o das cadeias. Os grupamentos metilenos das aminas DIPIP e aminoetilpiperazina proporcionaram um maior alongamento ?s poliamidas, aumentando a mobilidade das cadeias. O aumento do teor de etilenodiamina proporcionou o aumento das intera??es intermoleculares, causando o aumento do ponto de amolecimento, cristaliza??o, viscosidade e resist?ncia mec?nica da poliamida. ?cidos graxos dimerizados com diferentes composi??es tamb?m foram avaliados e, verificou-se que quanto maior o teor de mon?meros, menor ? a massa molecular da poliamida e, conseq?entemente, com menor viscosidade e mais quebradi?a. A maior pureza do ?cido graxo dimerizado promove a forma??o de uma fra??o ?nica de cristaliza??o e fus?o. A ades?o da poliamida, em geral, est? associada mais ? massa molecular da poliamida e as intera??es intermoleculares entre substrato e cadeias da poliamida.
17

Forma??o e estabilidade t?rmica de filmes ultrafinos de PMMA

Esteves, Christian Roberto Becker 30 August 2012 (has links)
Made available in DSpace on 2015-04-14T13:59:08Z (GMT). No. of bitstreams: 1 459262.pdf: 2820867 bytes, checksum: 267527b44523b14c7f271d43ab7cef2c (MD5) Previous issue date: 2012-08-30 / In this study, we investigated the formation and thermal stability of PMMA ultrathin films of different thicknesses h from 2 to 50 nm deposited on Si wafers. To observe the effects caused by temperature in the surface morphology annealings at predetermined times were performed in air, N2 or vacuum atmospheres, at temperatures above (150 ? C), closed to (100 and 120 ? C) or much below the glass transition temperature Tg at the material. We also studied the effect of pre-irradiation with high-energy ions (300 keV and 2 MeV of H+ and 18 MeV of Au7+) on the stability of the films in vacuum. The as deposited films showed smooth and uniform surfaces, except in a few movies which presented circular holes of sizes between 100 and 300 nm in some regions. We observed a slight crease in the roughness of the films with increasing thickness up to h ~ 10 nm. For films with h> 10 nm, the root mean square roughness (RRMS) tended to stabilize around ~ 0.25 nm. The films maintained in air at ambient temperature showed, in some cases an aging effect with a increase in roughness of up to 20% after 60 days. For short periods, no significant effect was observed RRMS increased in all films treated at temperatures near or above the Tg values for all kinds of atmospheres. The magnitude of variation was however larger for films treated in vacuum. The roughness of the films reached up to three times the initial value after a period of 24h. In general, the thinnest films were more stable in all different environments. This result may be linked to the predominance of polar interactions at the interface (between the native oxide on the silicon surface and COO groups of PMMA) in the ultrathin films. The irradiation with heavy ions increased the roughness of the films for all fluences used. Irradiation with light ions, in the two energies used, accelerated the destabilization of the films at low doses, but generated an improvement in stability in intermediate doses. These effects can be attributed to the types of changes introduced by the ions in each energy regime. For the Au+ ion sputtering and damage are much more pronounced favoring formation of holes in the surface. For H+ ions predominate the diluted effects of secondary electrons that can increase the mobility of the chains via the fission process at low fluences, but eventually favoris crosslinking and stabilization for higher doses / Nesse trabalho foi investigada a forma??o e estabilidade t?rmica de filmes ultrafinos de PMMA de diferentes espessuras (entre 2 e 50 nm) depositados sobre l?minas de Si. Para observar os efeitos ocasionados por tratamento t?rmico nos diferentes ambientes na morfologia da superf?cie, foram realizados recozimentos em tempos pr?-determinados em ar, N2 e v?cuo, em temperaturas acima (150 C?), pr?ximas (100 e 120?C) ou muito abaixo da temperatura de transi??o v?trea Tg nominal do material. Tamb?m foi estudado o efeito de pr?-irradia??o com ?ons de alta energia (300 keV e 2 MeV H+ e 18 MeV Au+) na estabilidade dos filmes para tratamento t?rmico no v?cuo. Os filmes como depositados apresentaram superf?cies lisas e uniformes com exce??o de alguns filmes, em geral mais grossos, que apresentaram buracos circulares de tamanhos entre 100 e 300 nm em algumas regi?es. Observou-se um aumento suave da rugosidade dos filmes com o crescimento da espessura h dos filmes at? h~10 nm. Para filmes com espessura h>10 nm a rugosidade quadr?tica m?dia (RRMS) do filme tendeu a estabilizar em torno de ~0,25 nm. Os filmes mantidos em ar na temperatura ambiente mostraram em alguns casos efeito de envelhecimento com aumentos na rugosidade de at? 20% num per?odo de 60 dias. Para per?odos curtos, n?o se observou um efeito significativo nos filmes. Os filmes que foram tratados em temperatura acima ou pr?ximo da Tg apresentaram crescimento nos valores de RRMS para todos os tipos de atmosferas. A magnitude de varia??o foi, contudo mais acentuado para o v?cuo: a rugosidade dos filmes chegou a alcan?ar um valor at? tr?s vezes o valor inicial ap?s um per?odo de 24h. No geral, os filmes mais finos se mostraram mais est?veis frente aos tratamentos t?rmicos em todos os diferentes ambientes. Esse resultado pode estar vinculado ao predom?nio das intera??es de interface polares (entre o ?xido nativo na superf?cie do sil?cio e os grupos C=O do PMMA) nos filmes ultrafinos. A irradia??o com ?ons pesados aumentou a rugosidade dos filmes para todas as flu?ncias utilizadas. Por sua vez a irradia??o com ?ons leves nas duas energias utilizadas acelerou a desestabiliza??o dos filmes em doses baixas, mas gerou uma melhoria na estabilidade em doses intermedi?rias. Esses efeitos podem ser atribu?dos aos tipos de modifica??es introduzidas pelos ?ons em cada regime de energia. Para os ?ons de Au+ os danos e o sputtering s?o muito mais acentuados, favorecendo forma??o de buracos na superf?cie. Para ?ons de H+, predominam os efeitos dilu?dos dos el?trons secund?rios que podem aumentar a mobilidade das cadeias via processo de cis?o em flu?ncias baixas, mas favorecem a reticula??o e estabiliza??o para doses mais altas.
18

Identification and Characterization of Bovine Pol III Promoters to Express a Short-Hairpin RNA

Peoples, Michael D 1978- 14 March 2013 (has links)
The use of molecular biology as a means to advance agriculture has proven beneficial in many fields. However, the development lentiviral vectors that utilize a livestock promoter to express short hairpin RNA (shRNA) has been limited to date. The goal of this research project was to develop and characterize lentiviral bovine Pol III mir30 shRNA expression vectors for future use in livestock research. The bovine Pol III promoter (7sk, U6-2, or H1) was inserted directly upstream of a mir30 shRNA expression sequence in the lentiviral vector pNef-GT. A transient luciferase knockdown assay in human embryonic kidney (HEK) 293T cells was used to compare the functionality of these vectors. The bPol III mir30 shRNA expression vector was co-transfected with the pGL3 luciferase expression vector and the renilla expression vector pLB at a ratio of 5:10:1 respectively. The vectors were allowed 48 hrs to produce their respective products before luciferase activity was measured with the Stop-n-Glo Assay (Promega). Each bPol III promoter was able to express a functional shRNA resulting in a reduction of luciferase activity greater than 68 percent. The bH1 and bU6-2 Luc shRNA vectors were the most effective vectors when transfected with >76 percent (p-value <0.05) reduced luciferase activity. To confirm that these promoters were functional after integration into a bovine genome, recombinant lentivirus was made from these vectors. These particles were then used to transduce a bovine kidney (MDBK) cell line that expressed luciferase. After transduction, transgenic cells were selected by the addition of the antibiotic, Geneticin to the culture media until a population of 100 percent bPol III expression cells were observed for two passages and luciferase activity was measured. The 7sk promoter was the most effective bPol III promoter that reduced luciferase activity in these cells by 72 percent (p-value <0.05), while the bU6-2 and bH1 were moderately effective at reducing luciferase levels (37 percent, 46 percent respectively). These experiments were the first to quantify the bovine Pol III promoter function after integration into a bovine genome. While variability was observed, for livestock based research, the b7sk lentiviral vector appears to be the best choice to express a shRNA from the genome of a bovine genome.
19

Stability of coupled van der pol oscillators and applications to gait control in simple animals /

Low, Lesley Ann. January 2002 (has links)
Thesis (Ph. D.)--University of Washington, 2002. / Vita. Includes bibliographical references (leaves 153-160).
20

A study of dynamics of coupled nonlinear circuits

Hernández, José Luis Sánchez. January 2004 (has links) (PDF)
Thesis (Ph. D.)--Mathematics, Georgia Institute of Technology, 2005. / Feodor Vainstein, Committee Member ; Dieci Luca, Committee Member ; Yi Yingfei, Committee Member ; Wang Yang, Committee Member ; Shui-Nee, Chow, Committee Chair. Vita. Includes bibliographical references.

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