Há relatos de que doses baixas de pentilenotetrazol (PTZ) apresentam um efeito ansiogênico em testes comportamentais que medem ansiedade. No labirinto em cruz elevado a droga reduz a porcentagem de entradas e o tempo gasto nos braços abertos. O clordiazepóxido (CDP), por outro lado, é uma droga que tem efeitos ansiolíticos, aumentando a exploração dos braços abertos do labirinto ao reduzir a aversão natural dos ratos aos braços abertos. Obtém-se um efeito similar quando o teste ocorre no escuro. Cafeína (CAF) é um composto estimulante que, aplicada em baixas doses, estimula a atividade motora no labirinto. O presente trabalho investiga o efeito de drogas gabaérgicas nas medidas de ansiedade obtidas no labirinto em cruz elevado, na presença ou ausência de luz. Ratos distribuídos aleatoriamente em 18 grupos receberam injeções de PTZ (0, 10 e 20 mg/kg), 5 min antes do teste, CDP (0,1.5 e 3 mg/kg) ou CAF (0, 10 e 30 mg/kg) 30 min antes do teste e foram colocados no labirinto em cruz elevado por 5 minutos, permitindo sua livre exploração, sob duas condições de luminosidade: claro (22 lux) ou escuro (0 lux). Os resultados mostraram que nem o PTZ nem o CDP tiveram qualquer efeito quando os animais eram testados no escuro. No claro, o PTZ diminuiu a exploração dos braços abertos, o que foi interpretado como um efeito ansiogênico, e o CDP apresentou um efeito oposto. A CAF provocou um aumento na exploração dos braços abertos somente no escuro. Esses resultados sugerem que a luminosidade deflagra as respostas responsáveis pela esquiva dos braços abertos, e as drogas simplesmente aumentam ou bloqueiam esse efeito. Da mesma forma, a falta da aversão desencadeada pela luz permitiu que a atividade locomotora aumentasse sob ação da CAF. / Pentylenetetrazol (PTZ), in low doses, was reported to have an anxiogenic effect in behavioral tests measuring anxiety. In the elevated plus-maze, it reduces the percentage of entries into and the time spent in the open arms. Chlordiazepoxide (CDP), on the other hand, is an anxiolytic drug which increases open arm exploration in the elevated plus-maze by reducing the natural aversion of rats to the open arms. A similar effect is obtained by testing rats in the dark. Caffeine (CAF) is a stimulant compound that, when administered in low doses, stimulates locomotor activity in the plus-maze. The present work investigated the effect of gabaergic drugs on anxiety levels in the presence and absence of light. Rats were randomly divided into 18 groups and either injected 5 min before testing with PTZ (0, 10, 20mg/kg) or 30 min before testing with CDP (0, 1.5, 3.0 mg/kg) or CAF (0, 10 and 30 mg/kg) and allowed to freely explore an elevated plus-maze for 5 min under two illumination conditions: light (22 lux) or dark (0 lux). Results show that neither PTZ nor CDP had any effect when the animals were tested in the dark. When tested in the light, PTZ decreased exploratory behavior in the open arms, which is usually interpreted as an anxiogenic effect, while CDP had the opposite effect. CAF provoked an increase in open arm exploration but only when the rats were tested in the dark. These results suggest that light triggers the responses responsible for the avoidance of the open arms, and the drugs simply enhance or block them. Likewise, lacking the aversion triggered by light allowed locomotor activity to increase under the action of CAF.

Chemical thrusters are widely used in spacecraft for attitude control and orbital manoeuvres. They produce a plume of neutral gas which produces ions via photoionisation and charge exchange. Measurements of local plasma properties will be aff ected by perturbations caused by the coupling between the newborn ions and the plasma. A model of neutral expansion has been used in conjunction with a fully three-dimensional hybrid code to study the evolution and ionisation over time of the neutral cloud produced by the ring of a mono-propellant hydrazine thruster as well as the interactions of the resulting ion cloud with the ambient solar wind. A parameter survey was performed for varying angles of injection and injection rates, particle kinetics were also investigated. Results are presented which show that the plasma in the region near to the spacecraft will be perturbed for an extended period of time with the formation of an interaction region around the spacecraft, a moderate amplitude density bow wave bounding the interaction region and evidence of an instability at the forefront of the interaction region which causes clumps of ions to be ejected from the main ion cloud quasi periodically and the ways in which these features are modifi ed by the degree of solar wind mass loading and the relative orientation of the magnetic fi eld to the angle of injection. This may a ffect Solar Probe Plus for a signifi cant duration as data taking and delicate sensory equipment may be required to cease operation until local fluctuations return to a more moderate level. The scale of the fluctuations seen are dependent upon the duration of the thruster ring and the speci fic geometry and therefore e ffects may vary in-situ.

L'avion plus électrique est un concept qui a le vent en poupe chez les principaux constructeurs du domaine de l'aéronautique. Dans ce domaine, comme dans d'autres, les besoins en énergie électrique sont croissants et nécessitent de mettre en place des systèmes de conversion d'énergie fiables, performants et modulaires. Ces systèmes de conversion sont souvent couplés avec des systèmes de stockage d'énergie (type batterie) permettant dans certaines situations de rendre l'avion énergétiquement autonome grâce à une source de puissance indépendante des principaux organes de production d'énergie. Cette interconnexion batterie - réseau de bord présente un rapport de tension élevé ce qui, ajouté aux fortes valeurs de courant de la basse tension, en fait un objet particulièrement complexe à réaliser.L'objectif de cette thèse est de concevoir de manière optimale un convertisseur de puissance isolé permettant l'interconnexion d'un bus basse tension 28 V (typiquement des batteries) à un bus haute tension 540 V (réseau de bord de l'avion) avec une puissance échangeable d'environ 12 kW. Elle se déroule dans le cadre d'un projet ANR (quatre partenaires universitaires, associés à l'entreprise AIRBUS) dont l'une des tâches est le développement d'outils de conception pour l'électronique de puissance. Le travail correspondant comprend une contribution à cette tâche sous forme de la construction de modèles des principaux composants intervenant dans un convertisseur, modèles destinés à être intégrés dans les routines d'optimisation. Pour cette raison, ils seront analytiques (physique, empiriques, mélange des deux).Ces modèles seront ensuite insérés dans un outil global développé dans une autre thèse du projet, à l'aide duquel différentes architectures de convertisseurs seront comparées afin d'en déduire la meilleure solution pour le cahier des charges énoncé précédemment. Un prototype du convertisseur retenu sera finalement réalisé en utilisant des technologies avancées, pour conduire une validation expérimentale. / The electric aircraft tends to become widespread at all the main manufacturers of the domain of the aeronautics. Needs do not stop growing and require setting up reliable, efficiency and modular systems of conversion of energy. These systems of conversion are often coupled with systems of storage of energy (battery) allowing in certain situations to make the punctually autonomous aircraft energetically thanks to a source of power independent from main organs of power production. This interconnection battery - network of edge presents a very high report of rise of tension what, added to the high current value of the battery bus, in fact a particularly complex object to be realized.The objective of this thesis is to design in an optimal way a converter of power isolated allowing the interconnection of a low-voltage bus 28V (typically batteries) in a high-voltage bus 540V (network of edge of the aircraft) with an exchangeable power about 12 kW. It takes place within the framework of an ANR project (four university partners + AIRBUS) the development of tools of conception of which one of the tasks is for the ENP. The corresponding work includes a contribution to this task in the form of the construction of models of the main components occurring in a converter, model intended to be integrated into the routines of optimization. For that reason, they will be analytical (physical, empirical or mix both).These models will then be inserted into a global tool developed in another thesis of the project, by means of which various architectures of converters will be compared to deduct the best solution from it for the previous specifications. A prototype of the reserved converter will be finally realized by using advanced technologies, to lead an experimental validation.

Accurate modeling of mixed solvent electrolyte systems is difficult and is not readily available in property modeling software such as Aspen Plus. Support for modeling these systems requires the knowledge and input of parameters specific to the compounds in question. The need for these parameters is particularly relevant in simulating new designs based upon recent developments in a concept known as pass-through distillation (PTD). In support of a specific application of PTD, this work determines and validates with existing experimental data, accurate user-parameters for the eNRTL property model in the ternary system of ethanol, water, and lithium bromide. Furthermore, this work creates the foundation for simulating this new PTD process by modeling the removal of bioethanol from a fermentation broth using low temperature evaporation in conjunction with absorption and stripping units to omit the need of a condenser requiring refrigeration. This will enable future investigations into the applications of PTD as well as provide a foundation for modeling the ternary system of ethanol, water and lithium bromide.

It is of great importance to be able to unambiguously interpret deoxyribonucleic acid (DNA) profiles, especially with Low Template (LT) DNA and mixture DNA that may contain major and minor contributors. Reducing stochastic effects, such as heterozygote peak imbalance, dropouts, and stutter artifacts have been studied by scientists in order to improve the evaluation of low quality DNA profile. There has been much research on a compatible solute, trehalose, in its effectiveness in enhancing the polymerase chain reaction (PCR), especially with GC-rich templates of DNA, and thermal stabilizing Thermus Aquaticus (Taq) DNA polymerases. Based on previous research, the effect of trehalose on peak heights, peak height ratios, and stutter ratios (n-1) from 15 short tandem repeats (STR) loci of the AmpFℓSTR® Identifiler® Plus PCR Amplification Kit was evaluated with 0.025ng, 0.05ng, 0.1ng, and 1ng of DNA, through the addition of 0M (control), 0.2M, and 0.4M of trehalose for each quantity of DNA. Although there was an observation regarding changes in average peak heights at 1ng of DNA with the addition of 0.2M, and 0.4M of trehalose, no conclusions could be made with the average peak heights for 0.025ng, 0.05ng, 0.1ng, and 1ng of DNA. The reason is that the propagation of pipetting error during the preparation of each batch could have contributed to the difference in the amount of DNA between each conditions which can be directly reflected in peak heights. Furthermore, unexpected discrepancy between the average peak heights for 0.1ng of DNA from the first and the second experiments rendered 0.1ng of DNA incompatible for comparison. With regards to average peak height ratios for 0.025ng, 0.05ng, 0.1ng, and 1ng of DNA, and average reverse stutter ratios for 0.1ng, and 1ng of DNA, there were no evidence to suggest that 0.2M or 0.4M of trehalose had any effects. Consistent trends for 0.1ng (Exp. 1 and 2) and 1ng of DNA from a statistical analysis through one-way ANOVA of individual loci, suggested that trehalose may have varying effects on certain loci. However, this observation must be approached with caution as it is uncertain whether unique trends across each data sets for certain loci were observed by chance due to small sample sizes or due to mechanisms of stutters and trehalose that are currently unknown. Future studies regarding the effect of trehalose on peak heights should be done with more precision through minimizing pipetting error, which can be accomplished by preparing one batch from which aliquots are taken. The result of the research does not show enough evidence to prove the usefulness of trehalose since the addition of trehalose does not yield consistently higher average peak heights and peak height ratios, and lower average reverse stutter ratios across 15 STR loci. Therefore, our results do not support that 0.2M and 0.4M of trehalose are useful within the parameter of forensic DNA analysis as they do not enhance the polymerase chain reaction (PCR) and improve stochastic effects for DNA profiles.

Introdução: Materiais com modificações na formulação original do MTA, incluindo o NeoMTA Plus, têm sido desenvolvidos buscando melhorar suas propriedades físicoquímicas. O objetivo do presente estudo foi analisar a resposta do tecido ósseo de ratos Wistar frente ao implante de NeoMTA Plus em comparação com o MTA Angelus. Metodologia: 24 ratos foram anestesiados e uma broca de baixa rotação foi empregada para confeccionar três cavidades cirúrgicas no fêmur direito. As cavidades cirúrgicas foram preenchidas aleatoriamente com os materiais teste NeoMTA Plus e MTA Angelus ou deixada vazia (controle negativo). Após 7, 30 e 90 dias as características do reparo ósseo foram classificadas em escores com o auxilio de microscópio óptico. As comparações entre os grupos foram realizadas pelo teste de Friedman e as comparações entre os três períodos experimentais em cada grupo foram realizadas pelo teste de Kruskal-wallis, seguido pelo teste de Dunn. O nível de significância foi estabelecido em P<.05. Resultados: Não foram observadas diferenças estatísticas entre os materiais e o grupo controle nos 3 tempos experimentais. Em todos os grupos houve diferença estatisticamente significante entre os tempos de 7 e 30 dias e nenhuma diferença foi observada entre os tempos de 30 e 90 dias. Além disso, o reparo em 90 dias foi significativamente melhor do que em 7 dias nos grupos NeoMTA Plus e controle. Conclusões: O NeoMTA Plus demonstrou comportamento biológico satisfatório em contato direto com o tecido ósseo de ratos Wistar, apresentando resultados semelhantes aos grupos MTA Angelus e controle. / Introduction: Materials that alter the original formulation of MTA have been developed to improve physical-chemical properties, including NeoMTA Plus (NMTAP). The aim of the present study was to analyze bone tissue reactions to NMTAP compared to MTA Angelus (MTA-A). Methods: 24 animals were anesthetized and a slow-rotation bur was used to create three surgical cavities in their right femur. Surgical cavities were randomly designated to NMTAP, MTA-A and negative control (empty cavity) groups. After 7, 30, and 90 days histological analysis of the characteristics of bone repair were classified in scores. Friedman’s test performed intergroup comparisons and Kruskal-Wallis test, followed by the Dunn’s post hoc test, compared the three experimental periods in each group. The significance level was set at P<.05. Results: No differences were found between the materials and control group in each experimental period. In all groups, the bone tissue repair improved from 7 to 30 days and there was no difference from 30 to 90 days. Moreover, the repair at 90 days was significantly better than at 7 in NMTAP and control groups. Conclusions: NeoMTA Plus showed satisfactory biological behavior when in direct contact with rat bone tissue, presenting repair characteristics similar to those produced by MTA Angelus and by the control group.

Esta dissertação apresenta resultados da pesquisa de mestrado intitulada O Programa Mais Educação: Um estudo da implantação em uma unidade escolar paulista, cujo objetivo é analisar o PME em uma escola do Estado de São Paulo. O PME foi instituído no segundo governo de Luiz Inácio Lula da Silva, por meio da Portaria Interministerial de 24 de abril de 2007, e integra as ações do Plano de Desenvolvimento da Educação (PDE) lançado pelo Ministério da Educação (MEC), ao mesmo tempo em que a promulgação do Decreto nº. 6.094 instituiu o Programa de Metas Compromisso Todos pela Educação, mecanismo operacional do PDE. Tem como intuito induzir as escolas públicas de Ensino Fundamental do país à ampliação da jornada escolar e à organização curricular, na perspectiva da educação integral. A metodologia adotada para o alcance do objetivo proposto para a pesquisa, de cunho qualitativo, utiliza a coleta de dados em uma unidade escolar de Ensino Fundamental, pertencente à rede municipal de São Carlos. A pesquisa dialoga com concepções expressas nos documentos orientadores do PME e com documentos legais que o estruturam na escola, além de realização de entrevistas semiestruturadas com agentes responsáveis pelo programa e estudantes que o integraram. No contexto específico da escola, as limitações físicas e pedagógicas se destacaram. Verificamos a dificuldade de contratação de professores capacitados e a redução dos macrocampos. Além disso, havia necessidade de capacitação dos professores. Também, o espaço para a efetivação das atividades do programa na escola se restringiam a salas de aula, já que o local onde eram realizadas estas atividades não estava mais disponível. Aliadas a essas questões, havia a falta de integração entre o PME e a escola. Destacamos, também, a falta de interesse dos gestores municipais pelo programa, já que, não ofereciam o respaldo necessário para a sua efetivação. Apesar dos entraves na efetivação do PME, ele foi considerado, por entrevistados, uma ação positiva para os estudantes; estes por sua vez, expressaram gostar de integrar o Programa. / This masters thesis presents the results of the research entitled The Plus Education Program: A Study on the Implementation at a Scholar Unit in the State of São Paulo , whose goal is to analyze the PME in a school in the State of São Paulo. The PME was instituted at the second government of Luiz Inácio Lula da Silva, by the Interministerial Ordinance from April 24th 2007, and integrate the actions from the Development Education Plan (PDE) launched by the Ministry of Education (MEC), at the same time that the promulgation of the Decree n°6.094 established the Targets Program All for Education, operational mechanism from PDE. It aims to induce public Elementary Schools from the whole country to extent the school day and the curriculum organization, on the perspective of comprehensive education. The adopted methodology to achieve the goal proposed for the research, that presents a qualitative nature, collects data from an Elementary School unit that belongs to the municipality of the city of São Carlos. The research dialogues with conceptions expressed at orientating documents from PME and with legal documents that structure the school, beside semi structured interviews with responsible agents from the program and students that were part of it. On the specific context of the school, the physical and pedagogical constraints stood out. We verified the difficulty on hiring trained teachers and the reduction of the macro fields. In addition, there was the necessity of training the teachers. Also, the space for the accomplishment of the activities from the program was restricted to the classroom, since the place where these activities were performed was not available anymore. Combined to these questions, there was the lack of integration between the PME and the school. We also highlight the lack of concern in the program from municipal managers, since they did not support its execution. Despite the obstacles in the implementation of the PME, it was considered by one of the interviewed a positive action for the students, who in turn, expressed to like to integrate the Program.

La recherche de points communs entre des descriptions de données ou de connaissances est un problème de raisonnement fondamental en Machine Learning, qui a été formalisé par G. Plotkin dans les années 70s sous la forme du calcul du plus petit généralisant de ces descriptions. L'identification des plus petits généralisants a un large panel d'applications qui vont de l'optimisation de requêtes (e.g., pour matérialiser les points communs entre des requêtes lors de la sélection de vues ou pour factoriser leur exécution dans un contexte d'accès concurrentiel), à la recommandation dans le contexte des réseaux sociaux (e.g. pour créer de liens entre des utilisateurs basées sur leurs points communs selon leur profil ou leurs recherches). Dans cette thèse nous avons revisité la notion du plus petit généralisant dans le contexte de Resource Description Framework (RDF) et le fragment conjonctif de son langage de requêtes associé SPARQL, alias Basic Graph Pattern (BGP) queries. Contrairement à l'état de l'art, nous ne considérons aucune restriction, ni structurelle ni sémantique, sur les graphes et les requêtes. Nos contributions incluent la définition et le calcul des plus petits généralisants dans ces deux formalismes ce qui revient à trouver le plus grand ensemble de points communs entre des bases de données incomplètes et des requêtes conjonctives en présence de contraintes déductives. Nous proposons également une évaluation expérimentale de nos contributions. / Finding commonalities between descriptions of data or knowledge is a fundamental task in Machine Learning. The formal notion characterizing precisely such commonalities is known as least general generalization of descriptions and was introduced by G. Plotkin in the early 70's, in First Order Logic. Identifying least general generalizations has a large scope of database applications ranging from query optimization (e.g., to share commonalities between queries in view selection or multi-query optimization), to recommendation in social networks (e.g., to establish connections between users based on their commonalities between proles or searches), through exploration (e.g., to classify/categorize datasets and to identify common social graph patterns between organizations (e.g., criminal ones)). In this thesis we revisit the notion of least general generalizations in the entire Resource Description Framework (RDF) and popular conjunctive fragment of SPARQL, a.k.a. Basic Graph Pattern (BGP) queries. By contrast to the literature, we do not restrict the structure nor semantics of RDF graphs and BGPQs. Our contributions include the denition and the computation of least general generalizations in these two settings, which amounts to nding the largest set of commonalities between incomplete databases and conjunctive queries, under deductive constraints. We also provide an experimental assessment of our technical contributions.

Cílem této diplomové práce je praktické i teoretické seznámení uživatelů neparametrických metod s několika vybranými statistickými programy, v nichž je možné neparametrické analýzy provádět. Nedílnou součástí cíle je i porovnání těchto programů. V teoretické části jsou stručně popsány základy testování statistických hypotéz a teorie neparametrických testů. V praktické části jsou prozkoumány programy NCSS 2007, Statistica 7, SPSS 15, Systat 12, Stagraphics Centurion XV, Minitab 15, S-Plus 6.2, SAS 9.1 a StatXact 7. U každého software jsou popsány neparametrické testy, které obsahuje, a zjednodušeně i vkládání dat a výstupy. Nakonec jsou vybrané programy porovnány z několika hledisek. Všechny vybrané programy obsahují značné množství neparametrických testů a liší se většinou jen v detailech. Pro většinu analýz není potřeba hledat speciální programy a dají se použít všechny vybrané, pouze pro porovnávání rozptylů je nutné použít buď SAS nebo StatXact, protože v jiných programech ve výběru tyto testy nenalezneme.

La classification multi-label est une extension de la classification traditionnelle dans laquelle les classes ne sont pas mutuellement exclusives, chaque individu pouvant appartenir à plusieurs classes simultanément. Ce type de classification est requis par un grand nombre d’applications actuelles telles que la classification d’images et l’annotation de vidéos. Le principal objectif de cette thèse est la proposition de nouvelles méthodes pour répondre au problème de classification multi-label. La première partie de cette thèse s’intéresse au problème d’apprentissage multi-label dans le cadre des fonctions de croyance. Nous développons une méthode capable de tenir compte des corrélations entre les différentes classes et de classer les individus en utilisant le formalisme de représentation de l’incertitude pour les variables multi-valuées. La deuxième partie aborde le problème de l’édition des bases d’apprentissage pour la classification multi-label. Nous proposons un algorithme basé sur l’approche des k-plus proches voisins qui permet de détecter les exemples erronés dans l’ensemble d’apprentissage. Des expérimentations menées sur des jeux de données synthétiques et réelles montrent l’intérêt des approches étudiées. / Multi-label classification is an extension of traditional single-label classification, where classes are not mutually exclusive, and each example can be assigned by several classes simultaneously . It is encountered in various modern applications such as scene classification and video annotation. the main objective of this thesis is the development of new techniques to adress the problem of multi-label classification that achieves promising classification performance. the first part of this manuscript studies the problem of multi-label classification in the context of the theory of belief functions. We propose a multi-label learning method that is able to take into account relationships between labels ant to classify new instances using the formalism of representation of uncertainty for set-valued variables. The second part deals withe the problem of prototype selection in the framework of multi-label learning. We propose an editing algorithm based on the k-nearest neighbor rule in order to purify training dataset and improve the performances of multi-label classification algorithms. Experimental results on synthetic and real-world datasets show the effectiveness of our approaches.