The aim of this diploma thesis is to project a polyfunctional building including a gym, fitness centre, bar and apartments. The plan is conducted in the form of project documentation, containing all the problems related with this issue. The building is located in the town, Bystřice pod Hostýnem. Considering the functionality, the construction is divided into two parts – southern and northern part. The south of the building is designated for accomodation situated on four floors. The north one, including three floors, is intended for the gym, fitness centre and bar. The terrain of building site slopes down to south-east. The construction will be built of prefabricated reinforced concrete frame construction RIEDER with block filling YTONG containing contact insulation system. The building is constructed with two flat single-shell roofs. This project is primarily focused on functional, disposal and architectural aspect os polyfunctional house.

The thesis deals with a design of a new construction of a polyfunctional urban house. It concerns a corner plot near the busy Opuštená avenue. The building is supposed to supplement the intended housing estate. Designed to be suitable for domestic, administrative and commercial use while maintaining an effective budget, the property is to provide the residents as well as the location with a high-quality space.

The subject of this diploma thesis is the elaboration of the building project of Polyfunkční dům Sokolská 5. In the course of the diploma thesis is elaborated a summary technical report, report of building site and drawings for individual stages. The implementation of the waterproofing of the base structure and the reinforced concrete monolithic skeleton are subject to technological regulations and control and test plans. In this work the object time and financial plan, the use of machines and mechanisms and health and safety at work are also solved. For the main building objects, an item budget and a detailed schedule are prepared. Part of this work is also the calculation of the heat transfer through the envelope of the building.

Diploma Thesis „Polyfunctional building“. The work deals implementation project of polyfunctional building in Jihlava. Object is situated on Jihlava suburb in emerging part called „Horní Kosov“. Land is gently sloping from west to east. It continues for Buková street. The building has six floors and a basement. The basement is made from monolithic reinforced concrete. It is used as garages. Floors are made of ceramic bricks with isolation . First floor contains four commercial premises and main entry for housing part. Seventeen flats are situated from second to sixth floor. Sizes of apartments are 1+kitchen corner to 4+kitchen corner with terraces and balcony. Roofing is solved with simple flat roof. Heating will be ensured with central heating. Every apartment, commercial premises, shared premises are going to have it’s own gas boiler. Polyfunctional building is connected on a local road – Buková street, which is connected to new communication. Within multifunctional building twenty parking slots is designed at the basement. Next fourteen parking slots is designed outside.

The main aim of the thesis is to design a new building of the mixed-use building in Brno. The building consists of two parts. One part consists of shops. The second part consists of offices and conference space. It is a building that has three floors above ground and one underground. In the basement there are a facilities and a garage. Shops are designed on the ground floor. There are offices on the second floor. Conference rooms are on the third floor. The building has a flat roof. The vertical load-bearing structures are designed from Porotherm ceramic blocks. In the core of the building, the vertical load bearing structures are designed as monolithic reinforced concrete walls. Columns are used in the building for the possibility of open space. The masonry of the basement consists of white bathtub. The floors are designed as folded from the Spiroll system. Internal non-load-bearing walls are designed from gypsum board with double cladding. The second part of the work deals with forced ventilation, cooling and central heating. In the third part of the project I deal with acoustics. The project was carried out in the Auotocad program. All constructions comply with valid standards and regulations.

Monolithische Phasen werden seit ca. 20 Jahren entwickelt und sind in den letzten Jahren eine attraktive Alternative zu etablierten mit Partikeln gefüllten Säulen geworden. Sie werden in anorganische Phasen und organische Polymermonolithe unterteilt. Monolithe bestehen aus einem einzigen, durchgehenden Stück. Charakteristisch ist das sie durchziehende Porennetzwerk, durch das der Eluent mit geringerem hydraulischen Widerstand fließen kann und das somit schnellere Flussraten ermöglicht. Polymermonolithe werden vorwiegend für die Separation großer Biomoleküle aufgrund eines durch Konvektion beschleunigten Massentransfers eingesetzt. Zudem sind sie über einen breiten pH-Wert-Bereich stabil und können direkt (in situ) im gewünschten Format polymerisiert werden. In der vorliegenden Arbeit gelang die Herstellung neuartiger epoxidbasierter Phasen nach einem von Weller et al. entwickelten Konzept, die im Affinitätsexperiment angewendet wurden. Die Herstellung erfolgte durch Autopolymerisation polyfunktioneller Glycidylether. Für die Funktionalisierung wurden nicht polymerisierte Epoxide genutzt. Als Monomere dienten TEPIC, GE 100 sowie GE 500. Die Arbeiten konzentrierten sich vor allem auf die bei Raumtemperatur durchführbaren Synthesen mit dem höher funktionellen GE 500. Die Polymerisationsbedingungen wurden hinsichtlich Porogenmischung und -anteil optimiert. Eine mit 75 Vol.-% Porogen (Dioxan/ MTBE (2:3)) hergestellte und mit rProtein A funktionalisierte Kapillarsäule (66 %, 12 µm, 7m2/g) ergab im Affinitätsexperiment eine Kapazität von 0,44 mg/mL aus Kaninchenserum isolierbarem IgG. Durch Beimischung von 60 % BDE konnte der Epoxidgehalt vervierfacht und die Porengröße auf 400 nm bei 59 % Porosität reduziert werden. Die spezifische Oberfläche wurde verdreifacht und die Kapazität präparierter Disks auf 0,90 mg/mL etwa verdoppelt. Die in dieser Arbeit entwickelten Disks können zur Isolierung von IgG aus einer komplexen Probe, wie beispielsweise Blutserum, eingesetzt werden. / Monolithic supports have been developed since 20 years and have become an attractive alternative to well-established columns packed with particles over the past years. They are classified into inorganic media and organic polymer monoliths. Monoliths consist of a single, continuous piece with an integrated characteristic porous network through which the eluent can flow with lower hydraulic resistance and which consequently offers higher flow rates. Due to an accelerated mass transfer caused by convection polymer monoliths are mainly used for separation of large biomolecules. In addition, they are stable over a wide pH range and can be polymerized directly (in situ) in the desired format. In the present work the successful preparation of new epoxide-based supports according to a concept introduced by Weller et al. as well as their application in affinity chromatography are reported. Their preparation was carried out by self-polymerization of polyfunctional glycidyl ethers and for functionalization non-polymerized epoxide groups were used. As monomers TEPIC, GE 100 and GE 500 were utilized. The work has focused especially on the polymerization of the higher functional GE 500, which can be perfomed at room temperature and was optimized in terms of both composition and amount of porogen. The extraction of IgG from rabbit serum with a capillary column (66 %, 12 µm, 7m2/g) prepared by 75 vol.-% porogen (dioxane/ MTBE (2:3)) and functionalized with rprotein A resulted in a capacity of 0,44 mg/mL. By addition of 60 % BDE the epoxide content was quadrupled and the pore size reduced to 400 nm while maintaining consistently high porosity of 59 %. The specific surface area was tripled and the capacity of prepared disks approximately doubled to 0,90 mg/mL. The disks developed in this work can be applied for the isolation of IgG from complex samples such as serum.

The main contens of the thesis is solving a construction-technological project substructure and the upper shell construction of a polyfunctional building in Pardubice. Excavation and foundation work are done in section A and B1 and B2. Drilled piles are foundation of a polyfunctional building. Drilled piles are support of reinforced concrete grillage, which are from strips foundation and foundation pads. Cast-in-place concrete frame is a form of load-bearing structure, which contens plate ceilings, pillars and walls. Peripheral masonry is from hellow clay blocks, which are between cast-in-place concrete frame. Diploma thesis includes technical report, plan of building side, schedule and the budget of the building, technological instructions, radon prevent, control and testing plan, safety, balance resources, selected constructional details, machinery composition, bill of quantities.