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Ag2s/2-mpa Quantum Dots / Cytocompatibility And Cellular InternalizationErdem, Rengin 01 June 2012 (has links) (PDF)
Quantum dots are fluorescent semiconductor nanocrystals that have unique optical properties such as high quantum yield and photostability. These nanoparticles are superior to organic dyes and fluorescent proteins in many aspects and therefore show great potential for both in vivo and in vitro imaging and drug delivery applications. However, cytototoxicity is still one of the major problems associated with their biological applications.
The aim of this study is in vitro characterization and assessment of biological application potential of a novel silver sulfide quantum dot coated with mercaptopropionic acid (2-MPA). In vitro studies reported in this work were conducted on a mouse fibroblast cell line (NIH/3T3) treated with Ag2S/2-MPA quantum dots in 10-600 &mu / g/mL concentration range for 24 h. Various fluorescence spectroscopy and microscopy methods were used to determine metabolic activity, proliferation rate and apoptotic fraction of QD-treated cells as well as QD internalization efficiency and intracellular localization. Metabolic activity and proliferation rate of the QD treated cells were measured with XTT and CyQUANT® / cell proliferation assays, respectively. Intracellular localization and qualitative uptake studies were conducted using confocal laser scanning microscopy. Apoptosis studies were performed with Annexin V assay. Finally, we also conducted a quantitative uptake assay to determine internalization efficiency of the silver sulfide particles.
Correlated metabolic activity and proliferation assay results indicate that Ag2S/2-MPA quantum dots are highly cytocompatible with no significant toxicity up to 600 &mu / g/mL treatment. Optimal cell imaging concentration was determined as 200 &mu / g/mL. Particles displayed a punctuated cytoplasmic distribution indicating to endosomal entrapment.
In vitro characterization studies reported in this study indicate that Ag2S/2-MPA quantum dots have great biological application potential due to their excellent spectral and cytocompatibility properties. Near-infrared emission of silver sulfide quantum dots provides a major advantage in imaging since signal interference from the cells (autofluorescence) which is a typical problem in microscopic studies is minimum in this part of the emission spectrum.
The results of this study are presented in an article which was accepted by Journal of Materials Chemistry. DOI: 10.1039/C2JM31959D.
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A colloidal nanoparticle form of indium tin oxide: system development and characterizationGilstrap, Richard Allen, Jr. 06 April 2009 (has links)
A logical progression from the maturing field of colloidal semiconductor quantum dots to the emerging subclass of impurity-doped colloidal semiconductor nanoparticles is underway. To this end, the present work describes the formation and analysis of a new form of Tin-doped Indium Oxide (ITO). The form is that of a colloidal dispersion comprised of pure-phase, 4-6 nanometer ITO particles possessing an essentially single crystalline character. This system forms a non-agglomerated, optically clear solution in a variety of non-polar solvents and can remain in this state, at room temperature, for months and potentially, years. ITO is the most widely used member of the exotic materials family known as Transparent Conductive Oxides (TCOs) and is the primary enabling material behind a wide variety of opto-electronic device technologies.
Material synthesis was achieved by initiating a series of interrelated nucleophilic substitution reactions that provided sufficient intensity to promote doping efficiencies greater than 90% for a wide range of tin concentrations. The optical clarity of this colloidal system allowed the intrinsic properties of single crystalline ITO particles to be evaluated prior to their use in thin-films or composite structures. Monitoring the temporal progression of n-type degeneracy by its effects on the optical properties of colloidal dispersions shed light on the fundamental issues of particle formation, band filling (Burstein-Moss) dynamics, and the very origin of n-type degeneracy in ITO. Central to these studies was the issue of excess electron character. The two limiting cases of entirely free and entirely confined electron motion were evaluated by application of bulk-like band dispersion analysis and the effective mass approximation, respectively. This provided a means to estimate the number of excess conduction band electrons present within an individual particle boundary. The ability to control and optimize the level of n-type degeneracy within the colloidal ITO nanoparticle form by compositional variation was also demonstrated. A key to the widespread adoption of a new material by industry is an ability to produce multi-gram and perhaps, kilogram quantities with no significant sacrifice in quality. Accordingly, a modified synthesis process was developed to allow for the mass production of high-quality colloidal ITO nanocrystals.
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Structural and optical characterization of Si/Ge quantum dotsWigblad, Dan January 2008 (has links)
<p>In this study silicon-germanium quantum dots grown on silicon have been investigated. The aim of the work was to find quantum dots suitable for use as a thermistor material. The quantum dots were produced at KTH, Stockholm, using a RPCVD reactor that is designed for industrial production.</p><p>The techniques used to study the quantum dots were: HRSEM, AFM, HRXRD, FTPL, and Raman spectroscopy. Quantum dots have been produced in single and multilayer structures.</p><p>As a result of this work a multilayer structure with 5 layers of quantum dots was produced with a theoretical temperature coefficient of resistance of 4.1 %/K.</p>
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Colloidal nanocrystals with near-infrared optical properties : synthesis, characterization, and applicationsPanthani, Matthew George 05 April 2013 (has links)
Colloidal nanocrystals with optical properties in the near-infrared (NIR) are of interest for many applications such as photovoltaic (PV) energy conversion, bioimaging, and therapeutics. For PVs and other electronic devices, challenges in using colloidal nanomaterials often deal with the surfaces. Because of the high surface-to-volume ratio of small nanocrystals, surfaces and interfaces play an enhanced role in the properties of nanocrystal films and devices.
Organic ligand-capped CuInSe2 (CIS) and Cu(InXGa1-X)Se2 (CIGS) nanocrystals were synthesized and used as the absorber layer in prototype solar cells. By fabricating devices from spray-coated CuInSe nanocrystals under ambient conditions, solar-to-electric power conversion efficiencies as high as 3.1% were achieved. Many treatments of the nanocrystal films were explored. Although some treatments increased the conductivity of the nanocrystal films, the best devices were from untreated CIS films. By modifying the reaction chemistry, quantum-confined CuInSeXS2-X (CISS) nanocrystals were produced. The potential of the CISS nanocrystals for targeted bioimaging was demonstrated via oral delivery to mice and imaging of nanocrystal fluorescence.
The size-dependent photoluminescence of Si nanocrystals was measured. Si nanocrystals supported on graphene were characterized by conventional transmission electron microscopy and spherical aberration (Cs)-corrected scanning transmission electron microscopy (STEM). Enhanced imaging contrast and resolution was achieved by using Cs-corrected STEM with a graphene support. In addition, clear imaging of defects and the organic-inorganic interface was enabled by utilizing this technique. / text
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Self-assembled quantum dots in advanced structuresCreasey, Megan Elizabeth 09 July 2013 (has links)
Advances in nanofabrication have bolstered the development of new optical devices with potential uses ranging from conventional optoelectronics, such as lasers and solar cells, to novel devices, like single photon or entangled photon sources. Quantum encryption of optical communications, in particular, requires devices that couple efficiently to an optical fiber and emit, on demand, indistinguishable photons. With these goals in mind, ultrafast spectroscopy is used to study the electron dynamics in epitaxially grown InAs/GaAs quantum dots (QDs). Quantifying the behavior of these systems is critical to the development of more efficient devices. Studies of two newly developed InGaAs QD structures, quantum dot clusters (QDCs) and QDs embedded in photonic wires, are presented herein.
GaAs photonic wires with diameters in the range of 200 to 250 nm support only the fundamental HE11 guided mode. To fully quantify these new systems, the emission dynamics of QDs contained within wires in a large range of diameters are studied. Time correlated single photon counting measurements of the ground state exciton lifetimes are in very good agreement with predicted theoretical values for the spontaneous emission rates. For diameters smaller than 200 nm, QD emission into the HE11 mode is strongly inhibited and non-radiative processes dominate the decay rate. The best small diameter wires exhibit inhibition factors as high as 16, on par with the current state of the art for photonic crystals.
The QDCs are the product of a hybrid growth technique that combines droplet heteroepitaxy with standard Stranski-Krastanov growth to create many different geometries of QDs. The work presented in this dissertation concentrates specifically on hexa-QDCs consisting of six InAs QDs around a GaAs nanomound. The first ever spectral and temporal properties of QDs within individual hexa-QDCs are presented. The QDs exhibit narrow exciton resonances with good temperature stability, indicating that excitons are well confined within individual QDs. A distinct biexponential decay is observed even at the single QD level. This behavior suggests that non-radiative decay mechanisms and exciton occupation of dark states play a significant role in the recombination dynamics in the QDCs. / text
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Probabilistic modeling of quantum-dot cellular automataSrivastava, Saket 01 June 2007 (has links)
As CMOS scaling faces a technological barrier in the near future, novel design paradigms are being proposed to keep up with the ever growing need for computation power and speed. Most of these novel technologies have device sizes comparable to atomic and molecular scales. At these levels the quantum mechanical effects play a dominant role in device performance, thus inducing uncertainty. The wave nature of particle matter and the uncertainty associated with device operation make a case for probabilistic modeling of the device. As the dimensions go down to a molecular scale, functioning of a nano-device will be governed primarily by the atomic level device physics. Modeling a device at such a small scale will require taking into account the quantum mechanical phenomenon inherent to the device. In this dissertation, we studied one such nano-device: Quantum-Dot Cellular Automata (QCA).
We used probabilistic modeling to perform a fast approximation based method to estimate error, power and reliability in large QCA circuits. First, we associate the quantum mechanical probabilities associated with each QCA cell to design and build a probabilistic Bayesian network. Our proposed modeling is derived from density matrix-based quantum modeling, and it takes into account dependency patterns induced by clocking. Our modeling scheme is orders of magnitude faster than the coherent vector simulation method that uses quantum mechanical simulations. Furthermore, our output node polarization values match those obtained from the state of the art simulations. Second, we use this model to approximate power dissipated in a QCA circuit during a non-adiabatic switching event and also to isolate the thermal hotspots in a design. Third, we also use a hierarchical probabilistic macromodeling scheme to model QCA designs at circuit level to isolate weak spots early in the design process.
It can also be used to compare two functionally equivalent logic designs without performing the expensive quantum mechanical simulations. Finally, we perform optimization studies on different QCA layouts by analyzing the designs for error and power over a range of kink energies.To the best of our knowledge the non-adiabatic power model presented in this dissertation is the first work that uses abrupt clocking scheme to estimate realistic power dissipation. All prior works used quasi-adiabatic power dissipation models. The hierarchical macromodel design is also the first work in QCA design that uses circuit level modeling and is faithful to the underlying layout level design. The effect of kink energy to study power-error tradeoffs will be of great use to circuit designers and fabrication scientists in choosing the most suitable design parameters such as cell size and grid spacing.
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Development of Split-protein Systems for Interrogating BiomacromoleculesShen, Shengyi January 2013 (has links)
The specific interactions of macromolecules along with the activity of enzymes are central to all aspects of biology. It is well recognized that when the relative concentration or activity of macromolecules is perturbed, it can lead to human diseases. Thus, the development of simple methods for the detection of macromolecules and the activity of enzymes in complex environments is important for understanding biology. Moreover, the development of methods for measuring interactions allows for the testing of inhibitors that can be used as tools or drugs for improving human health. Towards this goal, a promising new method has been developed, which is the focus of this thesis, called split-protein reassembly or protein fragment complementation. In this method, a protein reporter, such as the green fluorescent protein or firefly luciferase, is dissected into two fragments, which are attached to designed adaptor proteins. The designed split-protein systems only produce a measurable signal, either fluorescence or luminescence, when a specific macromolecular interaction or activity is present. In this thesis, I have extended previous research on the direct detection of DNA using split-protein sensors utilizing a red fluorescent protein, dsRED from Discosoma that allows for multiplexed DNA detection. I have designed a new split-luciferase based sensor for detection of poly (ADP-ribose) or PAR, which plays a key role in the response to DNA damage and have applied it for monitoring the activity of poly (ADP-ribose) glycohydrolase that controls PAR levels in the cell. Furthermore, I have significantly expanded upon a three-hybrid split-luciferase system for identifying protein kinase inhibitors. I have designed and tested two orthogonal peptide based chemical inducers of dimerization based on BAD and p53mt conjugates. I have studied these chemically induced dimerization systems in detail in order to begin to provide a theoretical basis for the observed experimental results. Finally, in a less related area, I have developed methods for producing water soluble semiconductor nanoparticles called Quantum Dots (QDs), with potential application in biological imaging. I have developed methods for functionalizing the QDs with orthogonal peptides, which can be potentially used for the assembly of high affinity non-covalent QD targeted proteins.
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Ultrafast Quantum Control of Exciton Dynamics in Semiconductor Quantum DotsGamouras, Angela 23 September 2013 (has links)
Controlling the quantum states of charge (excitons) or spin-polarized carriers in semiconductor quantum dots (QDs) has been the focus of a considerable research effort in recent years due to the strong promise of using this approach to develop solid state quantum computing hardware. The long-term scalability of this type of quantum computing architecture is enhanced by the use of QDs emitting in the telecom band, which would exploit the established photonic infrastructure. This thesis reports the use of all optical infrared experimental techniques to control exciton dynamics in two different QD samples consisting of InAs/GaAs QDs and InAs/InP QDs within a planar microcavity. An infrared quantum control apparatus was developed and used to apply optimized shaping masks to ultrafast pulses from an optical parametric oscillator. Pulse shaping protocols designed to execute a two-qubit controlled-rotation operation on an individual semiconductor QD were demonstrated and characterized. The quantum control apparatus was then implemented in simultaneous single qubit rotations using two uncoupled, distant InAs/GaAs QDs. These optimal control experiments demonstrated high fidelity optical manipulation of exciton states in the two QDs using a single broadband laser pulse, representing a step forward on the path to a scalable QD architecture and showcasing the power of pulse shaping techniques for quantum control on solid state qubits. As an alternative to single QDs, which have very low optical signals, subsets of QDs within an ensemble can be used in quantum computing applications. To investigate the mediation of inhomogeneities in a QD ensemble, pump-probe experiments were performed on InAs/InP QDs within a dielectric Bragg stack microcavity. Two different excitation geometries showed that the angle dependence of the microcavity transmission allowed for the spectral selection of QD subsets with transition energies resonant with the cavity mode. The microcavity mitigated inhomogeneities in the ensemble while providing a basis for addressing QD subsets which could be used as distinguishable quantum bits. This thesis work shows significant advances towards an optical computing architecture using quantum states in semiconductor QDs.
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The Effects of Multi-exciton Interactions on Optical Cavity EmissionQi, XIAODONG 31 July 2012 (has links)
This thesis presents a theoretical study of the collective effects of a large number
of photon emitters coupled to optical cavities. The ensemble effects are accounted
for by considering both the light emitting and scattering by the photon emitters. It
suggests that, to correctly estimate the emitters ensemble coupled cavity mode, it
is necessary to consider the existence of the excited excitons ensemble and optical
pumps. This thesis shows that optical pumps can excite more excitons and scattering
channels as pumping power increases. The change in exciton population can lead to
comprehensive spectral behaviors by changing the cavity spectral shapes, bandwidth
and resonance positions, through the inhomogeneous broadening and frequencies repulsion
effects of collective emissions. The existence of the exciton ensemble can also
enhance optical coupling effects between target excitons and the cavity mode. The
target exciton, which has a relatively large coupling strength and is close to the cavity
peak, can affect the properties of the background dipoles and their coupling to
the cavity. All these collective effects are sensitive to the number, the resonances
distribution, and the optical properties of the background excitons in the frequency
domain and the property of the target exciton, if any. This study provides a perspective on the control of the optical properties of cavities and individual excitons through collective excitation. / Thesis (Master, Physics, Engineering Physics and Astronomy) -- Queen's University, 2012-07-30 14:51:15.914
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Étude des mécanismes de capture et de fuite des excitons dans les boîtes quantiques d'InAs/InPGélinas, Guillaume January 2008 (has links)
Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal
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