The diploma thesis deals with design and assessment of steel arched bridge with the semi through bridge deck placed on the third-class road. This steel structure is drawn up to bridge water barrier – the river Olše in Návsí. The distance between axes lay on abutments is 48,5 meters. The bridge´s supporting structure consists of two steel arches angled towards each other and fastened to the foundation blocks. The supporting beam of the bridge deck is tied to the arch by tie-rods. The tie-rods are designed to the V-shaped system.

This thesis deals with the design and assessment of the wooden footbridge. The structure is designed for the locality of the village Loučná nad Desnou acrros the river Desná. Span of the footbridge is 30m, and the width is 4m. The footbridge is covered with a saddle roof. The main supporting elements are two triple joint arcs with intermediate bridge deck. The bridge deck is made of two main beams and nine cross members, with always two longitudinal members in between them. Roofing is of nine cross-bindings, which are connected by purlins and top purlin. The stability of the whole structure is stiffened by the horizontal longitudinal stiffeners in the top and bottom surface and vertical longitudinal stiffener in the roof. The main load-bearing elements are made of glued laminated timber, the other elements are made of solid timber. Stiffeners are made of steel rods.

The aim of diploma thesis is creating calculation algorithms off single types unconventional heat transfer equipment and their aplication in thermal - hydraulic calculations in the industrial aplication . The main attention is paid to shell and tube heat exchangers with ROD and disc baffle system and radiation heat exchanger. All of these exchangers are used mainly in process of waste thermic destruction. The theoretical part of the work describes construction details of each heat exchanger and calculation algorithms of these equipments. The practical part of the work applies theoretic calculation alghortims in the industrial aplication which is design and rating. In the final part advantages and disadvantages of results have been assessed for construction and process aspects.

This diploma thesis deals with the generation of the different drope stampings in industrial process in MBNS Kovárna Company. The main purpose of this thesis is analysis of the industrial process and finding the main factors influences on the process. It especially deals with forgings of the shafts and also flanges with the similar quality of material 42CrMo4. Reasons of the disagreements are focused on surface insensibilities of the forging - crack. First theoretic part introduces company, meaning of the quality of material and describes tools used for finding reasons of the generation of disagreements. In the second practical part is described the whole industrial process and determination of the reasons of disagreements. In concluding part the results are analyzed and some correctional recommendations are suggested.

Current requirements in mechanical engineering require more accurate operations and more efficient technologies. The aim of this thesis is the analysis of the leveling of square rods. The main problem is the setting of the leveling machine for the specified material and geometric data so that the initially curved material, which passes through alternatively positioned offset rollers, is leveled as much as possible. The main factor in the leveling process is the plastification of the material used for the redistribution of the residual stress. Based on existing theo-retical knowledge in this field, programs are set up to simulate the passing of the rod through the leveling machine. Further, modifications leading to the improvement of the whole process are presented. Finally, there is a verification of the results which is made independently of the submitted solution and processed by the finite element method.

The masters thesis is focused on design of the device for measurement of the aerodynamic forces and moments acting on radiators mounted in the wind tunnel test section. The thesis also includes the analysis of the aerodynamic drag which is produced by radiators and the LabView application software development.

The rapid and accurate identification of the bacterium of the genus Lactobacillus, which are an important part of the normal gastrointestinal microflora and fermented dairy products are currently mainly used amplification methods. The aim of the study was to analyze the possibility of resolution of selected bacterial strains of the genus Lactobacillus, using the metod of polymerase chain reaction in the real time combined with high resolution melting curve analysis (qPCR HRM). It was tested five primers designed for qPCR-HRM analysis of lactic acid bacteria. The specificity of the primers was also verified simultaneously using bioinformatic analysis. On the basis of analysis of the DNA were selected as the most appropriate primers P1V1/P2V1, V3F/V3R and V6F/V6R. The suitability of the primers V3F/V3R and V6F/V6R was verified on a complex sample of food supplement from which the DNA was isolated using magnetic particles. The presence of bacteria of the genus Lactobacillus was performed using high resoluting melting analysis curves. The obtained results were in agreement with the information given by the manufacturer.

Probiotic food products - food additives contain different species of probiotic bacteria. Accurate species identification with their characteristics is very important from the view of products quality. Methods of DNA diagnostics are used for these purposes. In this thesis DNA was isolated from 4 probiotic products. The presence of bacterial of genus Lactobacillus and species L. acidophilus, L. casei, L. plantarum, L. rhamnosus were detected in three products by PCR. This information was in accordance with the data provided by the manufacturer. Two sets of primers were used for identification of species. Using other primers sequences of genes such as bsh, lai and odc were detected in DNA isolated from the products. Differences were estimated among products concerning the detection of lai gene Lactobacillus acidophilus.

Antimicrobial substances, or bacteriocins are substances produced by probiotic lactic acid bacteria. They have a positive effect on the gastrointestinal tract of humans and are especially suitable for the food, but also the pharmaceutical industry. The aim of the thesis was the molecular identification of lactic acid bacteria of the Lactobacillus genus, species and their subsequent inclusion PCR method. Using the PCR method were tested also genes responsible for the production of bacteriocins. To confirm the production of bacteriocins has been selected the microbiological method, agarose droplet spot-test. In the present study also included the bioinformatics part to assess the specificity and non-specificity of the primers using in Primer-BLAST program.

<p>Osolike muve predstavljaju veliku familiju dvokrilaca koja u ekosistemu ima važnu ulogu opra&scaron;ivača biljaka i razlaganja organske materije. One su takođe dobar indikator naru&scaron;enosti ekosistema.&nbsp; Stoga je poznavanje njihove taksonomije i<br />ekologije od izuzetnog značaja. Jedan od tri najbrojnija roda osolikih muva&nbsp; predstavlja rod <em>Eumerus</em>, čija revizija na Balkanskom poluostrvu je predmet ove disertacije. Balkansko poluostrvo predstavlja izuzetan centar raznovrsnosti živog<br />sveta, između ostalih familija i za osolike muve. Za rod<em> Eumerus</em>, ono je pored Srednje Azije i Južne Afrike, jedan od centara specijacije.Predstavnici roda Eumerus su uglavnom sitnije crne muve, ponekad sa crvenim trbuhom. Karakteri&scaron;u ih bele popra&scaron;ene&nbsp; trake na tergitima, dva reda trnova na metafemuru&nbsp; apikoventralno i uvučena gornja spolja&scaron;nja poprečna vena na&nbsp; krilu, često sa dva kratka ispusta na ispupčenoj strani, okrenuta ka spolja. Cilj ove disertacije bio je utvrđivanje tačnog broja&nbsp; vrsta na Balkanskom poluostrvu i razre&scaron;avanje njihove&nbsp; taksonomije. Kao rezultat, utvrđeno je 44 prisutne vrste na&nbsp; istraživanom području koje su na osnovu morfolo&scaron;ke sličnosti&nbsp; podeljene u deset grupa. Tri utvrđene vrste ne pripadaju&nbsp; nijednoj grupi. U toku ovog istraživanja otkriveno je 12 vrsta novih za nauku:<em> E. aurofinis, E. banaticus</em>, in litt., <em>E. <span id="cke_bm_182S" style="display: none;">&nbsp;</span><span id="cke_bm_185S" style="display: none;">&nbsp;</span>bicornis</em><span id="cke_bm_185E" style="display: none;">&nbsp;</span><span id="cke_bm_182E" style="display: none;">&nbsp;</span>, in<span id="cke_bm_183S" style="display: none;">&nbsp;</span> litt., <span id="cke_bm_186S" style="display: none;">&nbsp;</span><em>E. crassus</em><span id="cke_bm_186E" style="display: none;">&nbsp;</span>, <span id="cke_bm_187S" style="display: none;">&nbsp;</span><em>E. kar<span id="cke_bm_183E" style="display: none;">&nbsp;</span>yates</em><span id="cke_bm_187E" style="display: none;">&nbsp;</span>, in li<span id="cke_bm_188S" style="display: none;">&nbsp;</span>tt., <em>E. montanum</em>, <em>E.&nbsp;<span id="cke_bm_188E" style="display: none;">&nbsp;</span> montenegr<span id="cke_bm_184E" style="display: none;">&nbsp;</span>inus, E. <span id="cke_bm_189E" style="display: none;">&nbsp;</span>nigrorufus</em>, in litt.<em>, E. phaeacus</em>, in litt., <span id="cke_bm_202S" style="display: none;">&nbsp;</span><em>E. rubrum, E. tenuitarsis</em><span id="cke_bm_202E" style="display: none;">&nbsp;</span>, in litt. i<em> E. torsicus.</em> Geometrijska morfometrija krila je d<span id="cke_bm_203E" style="display: none;">&nbsp;</span>ala odlične rezultate za razdvajanje&nbsp; kriptičnih taksona u okviru roda <em>Eumerus,</em> primenjena na&nbsp; vrstama grupe minotaurus. Utvrđeni su najznačajniji morfolo&scaron;ki karakteri za razlikovanje vrsta. Jedan od veoma&nbsp; značajnih rezultata ove disertacije je ključ za identifikaciju&nbsp; vrsta roda<em> Eumerus</em> na Balkanskom poluostrvu. Na osnovu morfolo&scaron;ke analize donet je zaključak da tricolor grupa&nbsp; predstavlja morfolo&scaron;ki zasebnu taksonomsku celinu sa jasnim&nbsp; indicijama da je njen rang iznad grupe vrsta. Centre diverziteta&nbsp; vrsta roda <em>Eumerus</em> na istraživanom području predstavljaju, prema bogastvu vrsta grčka ostrva Lezbos i Krf i jugoistočni&nbsp; deo Crne Gore uključujući Skadarsko jezero. Na osnovu &Scaron;enonovog indeksa diverziteta, područja sa najvećim indeksom su sledeća: ostrva Hios, Lezbos i Krf, zatim Peloponez i veći&nbsp; deo Crne Gore.</p> / <p>Hoverflies represents a large family of Diptera that have in the ecosystem an important role of pollinators and decomposer of organic matter. They are also a good indicator of ecosystem disruption. Therefore, knowledge of their taxonomy and ecology is of great importance. One of the three most numerous genus of hoverflies is the genus<em> Eumerus</em>, whose revision in the Balkan Peninsula is the subject of this dissertation. The Balkan Peninsula is an exceptional center of biodiversity, for&nbsp; hoverflies among other families. For the genus&nbsp;<em> Eumerus</em>, Balkan Peninsula is next to&nbsp; Central Asia and South Africa, one of the centers of speciation. Representatives of the genus&nbsp; Eumerus&nbsp; are mostly small black flies, sometimes with red abdomen. They are characterized by white stripes on the tergites, two rows of spines on the metafemur apico-ventrally and recessed upper outer transverse wing vein. The aim of&nbsp; this dissertation was to determine the number of species on the Balkan&nbsp; Peninsula and to resolve their taxonomy. As a result, 44 species were identified in the investigated area, which are divided into ten groups based on the&nbsp; morphological similarity. The three species identified do not belong to any group. In the course of this study, 12 new species were discovered for the science:&nbsp; <em>E. aurofinis, E. banaticus,</em> in litt.,&nbsp; <em>E. bicornis</em>, in litt.,&nbsp;<em> E. crassus,&nbsp; E. karyates</em>, in litt.,&nbsp; <em>E. montanum,&nbsp; E. montenegrinus,&nbsp; E. nigrorufus,</em> in litt.,&nbsp; <em>E. phaeacus</em>, in litt.,&nbsp; <em>E. rubrum,</em> <em>E. tenuitarsis</em>, in litt. and&nbsp; <em>E. torsicus</em>. The geometric&nbsp; morphometry of the wings gave excellent results for the separation of cryptic taxons within the genus&nbsp; Eumerus, applied to the species of the&nbsp; minotaurus&nbsp; group. The most important morphological characters for differentiating species were determined. One of the very important results of this dissertation is the identification key for species of the genus&nbsp; <em>Eumerus&nbsp;</em> on the Balkan Peninsula. Based on morphological analysis, conclusion is that the tricolor&nbsp; group represents a morphologically separate taxonomic entity with clear indications that its rank is above a group of species. The centers of diversity of the genus&nbsp;<em> Eumerus</em>&nbsp; in the research area represent, according to the species richness of the Greek island of Lesbos and Corfu and the southeastern&nbsp; part of Montenegro including the Skadar Lake. Based on the Shannon&rsquo;s Diversity Index, the areas with the highest index are as follows: the islands of Hios, Lesbos and Corfu, then Peloponnese and most of Montenegro.</p>