The part of RNA2 genome segment of several isolates of Radish mosaic virus (RaMV) including capsid protein genes was sequenced. Variability of capsid protein genes among the isolates of Radish mosaic virus was studied.

Este trabalho objetivou determinar as modificações ambientais causadas pelo uso de filme de polietileno de baixa densidade (PEBD), em túneis baixos com perfurações de 0%, 5%, 10%, 15% e 20% no desempenho da cultura da rúcula (Eruca vesicaria sativa (Mill) Thell) durante o verão e o outono e da cultura do rabanete (Raphanus sativus L.) durante o inverno e a primavera. Como variáveis resposta analisaram-se a umidade relativa do ar, a temperatura ambiente, a temperatura do solo, a radiação solar, além das características morfológicas da cultura: a massa seca, a massa verde, número de folhas (altura, peso, largura da planta), além da produtividade por área em cada tratamento. Os resultados mostraram que as variáveis climáticas envolvidas não apresentaram resultados significativamente diferentes entre os diversos níveis de perfuração pesquisados (5%, 10%, 15% e 20%). No entanto, as alterações microclimáticas ocorridas pela influência das perfurações no filme plástico tornaram o microclima no interior dos túneis baixos propício ao melhor desenvolvimento da cultura da rúcula (Eruca vesicaria sativa (Mill)Thell), e do rabanete (Raphanus sativus L.), resultando em maior produtividade. / The purpose of this research was to determine the environmental changes that the low density polythylene with different perforated film area (5, 10, 15, 20%) may cause on the rocket press culture (Eruca vesicaria sativa (Mill) Thell) during summer and fall time and radish culture (Raphanus sativus L.) during winter and spring time in low tunnel conditions. The climate variables analyzed were the humidity and air temperature, soil temperature and solar radiation. The culture parameters used to compare the treatments were dry matter, green matter, morphologicals characteristics of the plants (number, height, width and weight of leaves) and productivity. The results showed that find any significative differences between the climate variables inside the low tunnel with different perforated film area (5, 10, 15, 20%). However, the modification caused by the low tunnel with perforated plastic did provide performance of rocket press culture (Eruca vesicaria sativa (Mill) Thell) development in summer and fall time, and radish culture (Raphanus sativus L.) development in winter and spring, had better productivity and marketable characteristics.

No description available.

Non-point source pollution caused by erosion from road construction poses hazardous environmental effects. Percolation and infiltration of nutrients into groundwater can also be detrimental to the surrounding environment. In addition, annual roadside maintenance budget exceeded $14 million in 2011 for Mississippi. Objectives of this research were to evaluate rapidly established short-statured species in an effort to prevent erosion, combat non-point source pollution, reduce mowing cost, and provide quick cover following propagation. Factors evaluated were rate of establishment, plant cover, and mowing requirement. MDOT’s standard seed mix was evaluated along with Pennington’s SlopeMaster product and different combinations of selected plant species. Visual and image analysis showed oilseed radish plants established the quickest and provided the most cover. All sod treatments provided instant cover while Pennington’s Slopemaster product, as well as mixes that contained bermudagrass or bahiagrass, provided sufficient cover, but not in a timely manner.

Improved N management is required to enhance crop productivity, while reducing concomitant losses. Research was conducted for 4-y studying winter cover crops. Three legume species grown alone or with either cereal rye (Secale cereale) or ‘Tillage Radish®’ (Raphanus sativus L.) were used to quantify cover crop biomass and N content plus their effects on corn grain yield and N recovery. The effects of these cropping systems on selected soil health indicators was also determined. Rye bicultures enhanced biomass production, but antagonistically affected corn performance. Radish inclusion resulted in equal or greater cover crop N than rye. In year 3, the addition of radish across legume species increased corn N content (10.6 kg ha-1) and grain yield (1050 kg ha-1). Although cover crops did not affect soil bulk density, both bicultures increased soil C/N. The legume-radish association offers a novel practice towards improving crop performance and soil quality.

No description available.

Resumo: O presente trabalho teve como objetivo avaliar 100 progênies de meios irmãos da população de nabo forrageiro (Raphanus sativus L. var. oleiferus Metzg.), cultivar CATI AL 1000 em dois ciclos de seleção entre e dentro de progênies de meios irmãos, para as características: ciclo das plantas, altura das plantas, produtividade de grãos, massa de 1000 grãos, teor de óleo no grão e produtividade de óleo, na Fazenda Experimental São Manuel, no município de São Manuel - SP, no ano de 2004, e na Fazenda Experimental Lageado, no município de Botucatu - SP, no ano de 2005, 2006, e 2007, pertencentes a Faculdade de Ciências Agronômicas - campus de Botucatu - SP, da Universidade Estadual Paulista "Júlio de Mesquita Filho". Foram avaliadas 100 progênies de meios irmãos de nabo forrageiro cultivar CATI AL 1000, em ensaio de competição, distribuídas em delineamento látice 10x10, parcialmente balanceado, com três repetições. As progênies superiores foram recombinadas e selecionadas no ano agrícola seguinte obtendo um ciclo a cada dois anos. Foram realizados dois ciclos de seleção. Os dados coletados a campo, para todas as características, foram padronizados seguindo a metodologia recomendada, e submetidos a análise de variância e testes de médias. Foram obtidas as correlações entre os caracteres e obtidas as estimativas de parâmetros genéticos. Para todas as características avaliadas, foi identificada a presença de variabilidade genética, decrescendo no segundo ciclo de seleção, entre as progênies de meios irmãos. Foram observados ganhos genéticos de 8,96, 18,47, 93,29, 12,51, e 69,11 %, para as características avaliadas: altura das plantas, massa de 1000 grãos, produção de grãos, teor de óleo do grão, e produtividade de grão, respectivamente. Observou-se correlação negativa e significativa entre produtividade de grãos e teor de óleo... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The purpose of the present research was evaluate100 half-sib progenies of the population of wild radish (Raphanus sativus L. var. Oleiferus Metzg.) Cultivar CATI AL 1000 in two cycles of selection, among and within half-sib progenies for characteristics: cycle of plants, plant height, grain yield, 1000 grain weight, grain oil content and oil yield in the Experimental Farm San Manuel, in Sao Manuel - SP, in 2004, and in the Farm Experimental Lageado, in Botucatu - SP, in 2005, 2006 and 2007, at Faculdade de Ciencias Agronomicas - Botucatu campus - SP, Universidade Estadual Paulista Julio de Mesquita Filho". In this study, were evaluated 100 half-sib progenies of wild radish cultivar CATI AL 1000, in an essay competition, distributed in a 10x10 lattice, partially balanced, with three replications. The selected progenies were recombined and selected in the following crop year obtaining a cycle every two years. There were two cycles of selection. For all traits, the data collected at the field were patterned according to recommended methodology, and subjected to analysis of variance and multiple comparisons. The correlations between the characters and the estimates of genetic parameters were obtained. For all traits, the presence of genetic variability was identified, decreasing in the second cylce of selection, among the half-sib progenies. Genetic gains of 8.96, 18.47, 93.29, 12.51 were observed, and 69.11% for the characteristics evaluated: plant height, 1000 grain weight, grain yield, oil content of grain, and grain yield, respectively.There was negative correlation between grain yield and grain oil content, plant height and mass of 1000 grains, and positive and significant relationship between grain yield and plant height, also 1000 grain weight and oil content of grain. The genetic parameters values decreased from cycle I to cycle II, but the high magnitudes calculated in the second... (Complete abstract click electronic access below) / Orientador: Maurício Dutra Zanotto / Coorientador: José Geraldo Carvalho do Amaral / Banca: Cibelle Chalita Martins / Banca: João Paulo Teixiera Whitaker / Banca: Luciano Soares de Souza / Banca: Fábio Suano de Souza / Doutor

Made available in DSpace on 2014-06-11T19:30:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-12-19Bitstream added on 2014-06-13T19:00:28Z : No. of bitstreams: 1 sa_ro_dr_botfca.pdf: 609591 bytes, checksum: fbbd21f4b97ce442e120737b875fffb0 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / O presente trabalho teve como objetivo avaliar 100 progênies de meios irmãos da população de nabo forrageiro (Raphanus sativus L. var. oleiferus Metzg.), cultivar CATI AL 1000 em dois ciclos de seleção entre e dentro de progênies de meios irmãos, para as características: ciclo das plantas, altura das plantas, produtividade de grãos, massa de 1000 grãos, teor de óleo no grão e produtividade de óleo, na Fazenda Experimental São Manuel, no município de São Manuel – SP, no ano de 2004, e na Fazenda Experimental Lageado, no município de Botucatu – SP, no ano de 2005, 2006, e 2007, pertencentes a Faculdade de Ciências Agronômicas – campus de Botucatu – SP, da Universidade Estadual Paulista “Júlio de Mesquita Filho”. Foram avaliadas 100 progênies de meios irmãos de nabo forrageiro cultivar CATI AL 1000, em ensaio de competição, distribuídas em delineamento látice 10x10, parcialmente balanceado, com três repetições. As progênies superiores foram recombinadas e selecionadas no ano agrícola seguinte obtendo um ciclo a cada dois anos. Foram realizados dois ciclos de seleção. Os dados coletados a campo, para todas as características, foram padronizados seguindo a metodologia recomendada, e submetidos a análise de variância e testes de médias. Foram obtidas as correlações entre os caracteres e obtidas as estimativas de parâmetros genéticos. Para todas as características avaliadas, foi identificada a presença de variabilidade genética, decrescendo no segundo ciclo de seleção, entre as progênies de meios irmãos. Foram observados ganhos genéticos de 8,96, 18,47, 93,29, 12,51, e 69,11 %, para as características avaliadas: altura das plantas, massa de 1000 grãos, produção de grãos, teor de óleo do grão, e produtividade de grão, respectivamente. Observou-se correlação negativa e significativa entre produtividade de grãos e teor de óleo... / The purpose of the present research was evaluate100 half-sib progenies of the population of wild radish (Raphanus sativus L. var. Oleiferus Metzg.) Cultivar CATI AL 1000 in two cycles of selection, among and within half-sib progenies for characteristics: cycle of plants, plant height, grain yield, 1000 grain weight, grain oil content and oil yield in the Experimental Farm San Manuel, in Sao Manuel - SP, in 2004, and in the Farm Experimental Lageado, in Botucatu - SP, in 2005, 2006 and 2007, at Faculdade de Ciencias Agronomicas – Botucatu campus - SP, Universidade Estadual Paulista Julio de Mesquita Filho”. In this study, were evaluated 100 half-sib progenies of wild radish cultivar CATI AL 1000, in an essay competition, distributed in a 10x10 lattice, partially balanced, with three replications. The selected progenies were recombined and selected in the following crop year obtaining a cycle every two years. There were two cycles of selection. For all traits, the data collected at the field were patterned according to recommended methodology, and subjected to analysis of variance and multiple comparisons. The correlations between the characters and the estimates of genetic parameters were obtained. For all traits, the presence of genetic variability was identified, decreasing in the second cylce of selection, among the half-sib progenies. Genetic gains of 8.96, 18.47, 93.29, 12.51 were observed, and 69.11% for the characteristics evaluated: plant height, 1000 grain weight, grain yield, oil content of grain, and grain yield, respectively.There was negative correlation between grain yield and grain oil content, plant height and mass of 1000 grains, and positive and significant relationship between grain yield and plant height, also 1000 grain weight and oil content of grain. The genetic parameters values decreased from cycle I to cycle II, but the high magnitudes calculated in the second... (Complete abstract click electronic access below)

The relative amount of viral RNA of two comoviruses (RaMV and TuRSV) in different parts of plant during infection was determined. The two viruses were compared according to their ability to spread and multiply in plants.

Philosophiae Doctor - PhD / In different parts of the world, medicinal plants have demonstrated a lot of health benefits to mankind and remains an important source for the discovery of new bio-active compounds. Libya is a typical example of a country where medicinal plants are widely used. Plant extracts of five Libyan medicinal plants were used in this study to investigate their in vivo effects on spermatogenesis and steroidogenesis in male rats and on ovulation and fertility in female rats. The In vitro effects of these plant extracts were also investigated on TM3 Leydig cells and MCF 7 breast cancer cells. A phyto-chemical analysis of the five Libyan medicinal plants (flaxseed, black seeds, radish seed, date palm pollen and nutmeg) was done. The results showed that date palm pollen had a higher antioxidant activity than all of the above mentioned plants. In addition to this, Nigella sativa was observed to possess high flavonol content as well as high antioxidant activity. Male rats exposed to flaxseed, radish seeds and date palm pollen showed no significant alterations in body weight gain, whereas date palm pollen (240 mg/kg, p < 0.05) promoted an increase in body gain. This study also revealed a significant increase in the relative testicular weight of animals exposed to either flaxseed (300mg/kg) or date palm pollen (120mg/kg). In addition, the relative weights of the seminal vesicles of all treated groups showed significant increased values. The level of serum testosterone showed a significant increase after exposure to radish seed (80mg/kg) and a significant dose- dependent increase for date palm pollen when compared to control (P< 0.05). In contrast, flaxseed caused a dose-dependent significant (p <0.01) decrease in testosterone level at radish seed (300mg/Kg). All plant extracts caused a significant increase in sperm concentration. Sperm vitality significantly (p < 0.05) increased by radish seed (80mg/kg), flaxseed (300mg/kg) and date palm pollen (120, 240mg/kg) respectively. Total progressive motility improved significantly at flaxseed (300 mg/kg) (p < 0.001) as well as date palm pollen (p < 0.01). Histological examination of the cross sections of the testis showed clear presence of all stages of spermatogenesis in all the treated groups. Rat epididymides showed normal morphological appearance and their lumen were filled with spermatozoa. The diameter of seminiferous tubules in male rats exposed to date palm pollen (120 and 240 mg/kg) was significantly higher (p < 0.001). The heights of the germ cell epithelia within the eminiferous tubules were also significantly increased in all treated groups. Liver and renal functions tests showed a significant decrease in Alanine transaminase (ALT) and creatinine in all treated groups (p < 0.05), and this demonstrates the lack of cytotoxic effects of date palm pollen, radish seed and flaxseed on the rats. However, these plant extracts produced a non-significant (p > 0.05) increase in Aspartate transaminase (AST) levels. Besides this, superoxide dismutase activity (SOD) in testis was increased significantly by radish seed (160 mg/kg), flaxseed (200 mg/kg) and date palm pollen (120 mg/kg). There was also improved catalase activity in testis of male rats exposed to radish seed and date palm pollen. Regarding male sexual behavior, the time to reach the female and the mount frequency decreased significantly in male rats exposed to flaxseed (300 mg/kg) and date palm pollen (120 and 240 mg/kg; p > 0.05) thus, these plant extracts exhibit aphrodisiac properties. In addition, exposure of male rats to date palm pollen (120 mg/kg) produced a significant (p < 0.01) increase in the number of embryos in untreated female rats. In the female rats, the body weight gain was not affected (p > 0.05). However, the relative uterus weights exposed to nutmeg (200 mg/kg) and date palm pollen (120 and 240 mg/kg) were significantly decreased (p < 0.05). In addition, the relative weights of ovaries after treatment with nutmeg (400 mg/kg) and black seed (400 mg/kg) showed significantly increased values (p < 0.01). Serum FSH was significantly increased (p > 0.05 or 0.01) when the female rats have been exposed to black seed (200 mg/kg), nutmeg (200 mg/kg) or date palm pollen (120 mg/kg). The LH level significantly (p < 0.01) decreased following exposure to black seed (200 mg/kg), date palm pollen (120 mg/kg). On the other hand, serum LH concentration was significantly increased in female rats exposed nutmeg (400 mg/kg; p > 0.05). The creatinine activity in female rat serum in all treated groups was significantly decreased (p < 0.05). Whereas the higher dose of date palm pollen (240 mg/kg) caused only a non-significant decrease. ALT activity in serum of female rat exposed to either black seed (400 mg/kg) or date palm pollen (120 and 240 mg/kg) was shown to decrease significantly (p < 0.05). Histology of the reproductive organs, kidney and liver in the female rats showed no obvious alterations in any of the treated groups. In addition, the number of embryos in female rats significantly increased (p < 0.01; p < 0.001) following exposure of female rats to black seeds 400 and date palm pollen 240 mg/kg, respectively. Incubation of TM3 Leydig cells with radish seeds for 24, 48 or 72 hours caused a significant (p < 0.01) decrease in mitochondrial dehydrogenase activity. Besides that, date palm pollen and flaxseed increased the mitochondrial dehydrogenases activity of TM3 Leydig cells. In addition, higher concentration of date palm pollen, nutmeg and black seed were cytotoxic to MCF7 breast cells. In testis slices testosterone secretion in vitro was significantly increased by flaxseed (500 μg/ml; p > 0·05) and date palm pollen (500 μg/ml; p > 0·01). MCf-7 cells treated with BS 10-50 μg/ml black seed and nutmeg 10-50μg/ml significantly increased cell proliferation. However, the treatment with date palm pollen produced only a weak estrogenic effect, which resulted in a concentration dependent significant increase as observed between 50-1000 μg/ml date palm pollen. In conclusion, in this study, we observed that date palm pollen, radish seed and flaxseed increased libido as well as steroidogenesis and spermatogenesis, improved hepato and nephron-protective effects. In female rats, the plant extracts NM, BS and date palm pollen potentiated the production of gonadotropic hormones. In addition to this, at lower concentrations these medicinal plants promoted cell growth, whereas at higher concentrations they inhibited cell proliferation of MCF- 7 breast cancer cells. The anti-oxidant effects of these plant extracts have been implicated for the above mention effects.