In the context of the somatic mutation theory of chemical carcinogenesis, mutations are thought to arise during the replication of DNA past carcinogen-DNA adducts. The work described in this thesis deals with the testing of a hypothetical mechanism whereby mammalian cells are able to replicate their DNA past polycyclic aromatic hydrocarbon DNA adducts. The second objective of this thesis work was to develop both in vivo and in vitro models to study the induction of mutations in a target human gene by chemical carcinogens from two different classes, polycyclic aromatic hydrocarbons and nitrosamines. To approach the hypothetical mechanism of bypass replication in mammalian cells, synchronized Chinese hamster ovary cells were treated with the ultimate carcinogenic form of benzo (a) pyrene, 7β, 8α-dihydroxy-9α, 10α-epoxy-7,8,9,10-tetrahydrobenzo (a) pyrene (BPDE I). Using the pH step alkaline elution assay, it was found that the reduced rate of S phase progression was due to a delay in the appearance of multiple replicon size nascent DNA. It was determined using agarose gel electrophoresis that the ligation of Okazaki size replication intermediates was blocked in BPDE I-treated, synchronized CHO cells. The data obtained were, therefore, supportive of the 'block-gap' model of DNA bypass replication in carcinogen damaged mammalian cells. To study mutagenesis of a specific sequence induced by chemical carcinogens, the human c-Ha-ras proto-oncogene was transfected into the mouse fibroblast cell line, NIH 3T3. Transfected NIH 3T3 cell lines (HHRN 1-4) were isolated that had a low copy number of the human c-Ha-ras proto-oncogene and a non-transformed phenotype. It was determined that the integrated human c-Ha-ras gene was hypomethylated, and expressed at the messenger level. The human c-Ha-ras protein, p21, was also detected in these transfected cell lines. Treatment of the HHRN cell lines with the nitrosamine, N-methyl-N-nitroso-N'-nitroguanidine (MNNG) resulted in transformed NIH 3T3 foci. In vitro MNNG treatment of the plasmid, z-6, and transfection into NIH 3T3 cells led to the isolation of transformed cell lines. Screening of the in vitro and in vivo treated, transformed cell lines by RNA:RNA duplex mismatch analysis led to the detection of no mutations within the first exon of the human c-Ha-ras oncogene.

This research shed the light on regulations and their effects on operational risk management within the Swedish Swedish Banking Sector. The focus lies on operational risk management due to the introduction of new regulations such as FFFS 2014:1, FFFS 2014:4 and FFFS 2014:5. What could be found in the empirical analysis is that the regulations affected organizational changes.  Additionally, differences between large and small banks could be seen. All changes in terms of implementation of regulations are strongly performed throughout the Three Lines of Defence model where clear organization structure and work description are of importance. The Three Lines of Defence is tightly combined with the COSO framework and operational risk management to conduct compliant organization that is adaptable for any regulatory changes.

This research critically investigates the adoption and implementation of transfer pricing regulations in Nigeria with a particular emphasis on the petroleum sector. It opportunely chose Nigeria as a case study of transfer pricing issues in developing countries as Nigeria was devising and implementing its own transfer pricing regulation. In early 2012, Nigeria issued draft transfer pricing regulations for consultation with a view to publishing them at the end of the same year. In order to gauge the reaction of the stakeholders in Nigeria to the adoption and implementation of transfer pricing regulations and other related issues, a questionnaire was designed and administered to 140 respondents from eight different stakeholders groups including Nigerian tax authority, multinational companies in the petroleum sector and other organisations involved in tax matters. The questionnaire elicited their views on the (i) form of adoption of transfer pricing regulations; (ii) motive behind the adoption of the regulations; (iii) administrative resource capacity of the Nigerian tax authority; (iv) barriers that might hinder successful implementation of the regulations; and (v) needs for guidance and support. Institutional theory and resource-based view were employed as a theoretical lens through which to guide the study and to provide a platform against which to analyse the responses to the questionnaire and the interviews. The analysis of the responses to the questionnaire was thus undertaken subsequent to the adoption of the transfer pricing system which enabled informed reflection and critical analysis to be carried out on the results of the analysis. In addition, interviews with 16 experts were conducted subsequent to the issue and preliminary analysis of the responses to the questionnaire in order to gauge their reaction to the views being expressed by the respondents. This enabled a reflective analysis to be undertaken when assessing the information content emerging from the responses. The findings of the study indicate that the OECD transfer pricing framework, which is the transfer pricing system of choice amongst the developed countries, is not the most preferred framework for the regulation of transfer pricing in Nigeria. It also reveals that whilst the Nigerian tax authority has the administrative capacity to develop a transfer pricing team and other necessary platforms for the adoption and implementation of transfer pricing regulations, the lack of sufficient transfer pricing experts, political will and inadequate comparable information are the major potential barriers that might hinder the successful implementation of transfer pricing regulations in Nigeria. These findings should enable policy makers and other stakeholders in Nigeria to review their transfer pricing policies and find a way to overcome the identified potential barriers. This thesis is the first of its kind to empirically investigate the transfer pricing regulations in Nigeria with a particular emphasis on the petroleum sector. It also further establishes the use of institutional theory and resource-based view framework in transfer pricing studies and especially, by extending its application to the adoption and implementation of transfer pricing regulations with a particular emphasis on the petroleum sector.

The present thesis aims to investigate students’ willingness to share customer data in the fashion e-commerce. Special attention is given to the way trust, benefits and law regulations are discussed in this context. An inductive approach was applied utilizing focus group discussions. The goal of the empirical study was to gain insights in the way students argue about customer data and which feelings are involved. The study highlights findings in the key areas trust, benefits and law regulations. A shift of customer trust from brands to recommendations could be observed. In terms of benefits, focus group participants tend to choose value exchange over traditional rewards. Findings regarding law regulations suggest that the General Data Protection Regulation of the European Union can provide control of customer data but no security. Furthermore, consumer behavior plays an important role when it comes to data sharing attitudes. The experiences and observations of the participants may not be applicable to other studies. Furthermore, the study findings are bounded to the European Union. The findings suggest that trust, benefits and law regulations can influence students’ willingness to share customer data in the fashion e-commerce. The study is unique of its kind as it investigates the willingness to share customer data with the focus on students and fashion e-commerce. Hence, this research paper fills a gap in scientific literature and is valuable for businesses operating in the fashion e-commerce.

Denna uppsats syftar till att förklara och förstå de centrala faktorer som är av betydelse vid en implementering av ett finansiellt regelverk. Ett kvalitativt tillvägagångssätt har använts för att uppnå vårt syfte, genom att intervjua olika respondenter från olika typer av finansiella företag som står inför den kommande förändringen. Genom att kombinera vårt empiriska resultat med den framtagna teorin om implementeringar, har vi kunnat komma fram till faktorer som är av betydelse vid implementeringar av finansiella regelverk.  De mest betydelsefulla faktorerna för en framgångsrik implementering av finansiella regelverk är anpassningsbarhet, analysförmåga och kommunikation. Genom att redan tidigt följa regelverkets utveckling kan organisationen ha en god uppfattning om hur det senare kommer att utformas på en nationell nivå. På detta sätt blir inte implementeringsprocessen lika begränsad av den långa tid som det tar att stifta de nationella regleringarna. Det är väsentligt att organisationerna kan anpassa sin implementeringsmetod utifrån regelverkets innehåll, men även anpassat till dagens moderniseringen av tekniken. När det gäller att implementera regelverk är det oftast direkt kopplat till de utgivna tjänsterna, vilket sätter mer vikt på medarbetarnas kunskap och förståelse vid en sådan implementering. / This study seeks to explain and understand the key factors that are important when implementing a financial regulation.        A qualitative approach has been used to achieve our purpose, by interviewing different respondents from different types of financial companies facing the coming change. By combining our empirical results with the developed theory of implementations, we´ve been able to find factors that are important when implementing financial regulations.  The most important factors for a successful implementation of financial regulations are adaptability, analysis capability and communication. By following up early with the regulatory developments, the organization can have a good idea of ​​what it will look like when it’s finalized on a national level. In this way, the implementation process will not be limited by the long time that it takes to enact the national regulations. It is essential that organizations can adapt their implementation method based on the content of the regulation, but also adapt it to today's modernized technology. When it comes to implementing regulations, it is usually directly related to the services that are being offered, which puts more emphasis on the employees' knowledge and understanding.

The impact of domestic policy regulations and standards on trade has been at the forefront of global policy during the past decade. Every country develops their own policies and standards that differ from country to country. These differences create problems for manufacturing industries, especially in major exporting countries. This study overviews the policy context driving standards in the manufacturing industries. The study consists of three different articles that attempt to examine the role of technical regulations and standards and their relationship with trade using different econometric models In the first article, the standard factor endowment approach is employed to explain the effects of environmental regulatory policy on net exports in different manufacturing industries. The study hypothesizes that a countrys comparative advantage depends on its factor abundance. The regulatory policy increases production costs and, thus, reduces the output level of an industry. The results indicate that each industry is unique in the factors determining net exports and in many instances environmental regulations are important. In the second article, we investigate the impact of competition policy on a countrys production and export competitiveness. Since the impact of competition regulation depends upon the particular circumstances of the industry to which the policy is applied, we examine how competition policy impacts production and exports of a specific sector, in particular the agri-food processing sector. The results suggest that competition policy enhances competition by reducing entry barriers, and causes firms to produce more output with lower prices. Exports for both total and food manufacturing in the post-competition policy period are higher than exports in the pre-competition period. In the third article, we estimate regressions based on an extended gravity model to determine the possible influence of food safety standards on export flows of six Asia-Pacific countries to ten importing countries. We examine the relationship between bilateral exports and importers imposition of food safety standards. The results show that the value of exports in food and food products is negatively affected by food safety standards: the greater the aflatoxin standards, the lower its restrictiveness, and higher the bilateral export flows.

The intention of campaign finance regulations was to reduce the influence of special interest groups while increasing citizen contributions. Critics have suggested an unintentional consequence of this policy of increasing bias in campaign contributions in favor of incumbents. These claims of intentional and unintentional consequences, however, have rarely been tested. My dissertation examines the intentional and unintentional consequences of campaign finance regulations in the American states. This study adopts a theoretical framework emphasizing the different effects of regulations on two distinctive types of contributors. A particularistic contributor, whose motivation is influencing policy, is likely to be affected by contribution limits. A universalistic contributor, motivated by helping his or her favorite candidates, is not likely to respond to regulations. Furthermore, the disparity of contributions is not expected to be affected by contribution limits. Two specific hypotheses reflecting the theoretical consideration are tested: 1) Restrictive contribution limits reduce the number and amount of particularistic contributions and increase the disparity between the numbers as well as the amounts of contributions, and 2) Contribution limits do not affect the number, the amount, or the disparities of universalistic contributions. Individual contribution records on gubernatorial elections are collected from 1990 to 2000 in 42 states. After aggregating individual contribution records by state and candidate, two analyses are conducted at the state and candidate level. The results indicate that campaign finance regulations work without the unintentional consequence of providing a financial advantage to incumbents at both the state and candidate levels. Contribution limits increase the number of total contributors, reduce the number and amount of particularistic contributions, and increase the number of universalistic contributors. In addition, further analyses show a dynamic effect of contribution limits on corporations, labor unions, individuals, parties, and ideology PACs. Restrictive contribution limits reduce the number and amount of corporate contributions, but only reduce the amount of labor union contributions. On the other hand, strict contribution limits encourage individual contributions, but discourage party and ideological PAC contributions. The intentional consequence of campaign finance regulations does not result in the unintentional consequence of increasing bias in favor of incumbents. These findings suggest that current regulations that limit campaign contributions should remain in place.

In this thesis, we follow the incomplete contract model developed by Freixas and Rochet (1997). They model the decision of a bank regulator for continuing the sound bank or restructuring the failing bank as a game between bank equity owners, the bank manager, and the regulator under the incomplete contract framework. In this essay, we apply this incomplete contract model to Taiwan empirical data. After using several kinds of financial ratios as our indicators, we select nine ratios to process our empirical examination. And we find that: there are two banks appeared in the “ex-post efficient interference” segment except two ratios and also another two banks shown in the “ex-post efficient passivity” segment except one ratio. / In this thesis, we follow the incomplete contract model developed by Freixas and Rochet (1997). They model the decision of a bank regulator for continuing the sound bank or restructuring the failing bank as a game between bank equity owners, the bank manager, and the regulator under the incomplete contract framework. In this essay, we apply this incomplete contract model to Taiwan empirical data. After using several kinds of financial ratios as our indicators, we select nine ratios to process our empirical examination. And we find that: there are two banks appeared in the “ex-post efficient interference” segment except two ratios and also another two banks shown in the “ex-post efficient passivity” segment except one ratio.

<p>This study is about care managers, who process in the care of the elderly, regarding to needs, rules, regulations, guidelines and their freedom of space.</p><p> </p><p>The purpose with this study is to understand how the care managers process out of the basis from the needs of the elderly, the municipal guidelines in relation to the caremanagers freedom of space.</p><p>The study has a qualitative approach and is based on semistructured interviews with five different care managers.</p><p>To analyze our interview material we have use the street-level bureaucrat theory by Lipsky and Johansson and the theoretical idea<em> empowerment.</em></p><p> </p><p>Before we started our research about care managers we had an understanding in that the organization had an influence in the judgment of the needs of the elderly. Now we have an understanding in that the relative to the elderly is the one who wants to affect the care manager during the judgment of needs in what effort to make.</p><p> </p><p>Our study indicates that it exist insecurity regarding to the municipal guidelines, which was surprising. We thought that the guideline was something positive and a help full tool for the care managers.</p><p>The result in our research points out that the guidelines makes insecurity instead of comfort.</p><p>Our study shows that the care managers are contradictory regarding to their freedom of space. In the other hand they consider that their freedom of space is positive, but on the other hand when the municipal guidelines don´t work, they become worried and insecure.</p><p>It´s the none working municipal guidelines who gives the care managers a huge freedom of space. Which leads to that the care managers is afraid of doing misjudgments.</p><p> </p><p>Our result don´t distinguish from other studies made in this subject area.</p><p> </p>

The purpose of these studies was to determine whether lactogen-dependent Nb2-11c cells and lactogen-independent Nb2-SP cells differ with respect to morphology and autocrine growth control. To this end, the ultrastructural and surface morphology of both Nb2 cell lines was analyzed and the autocrine growth modulatory activity of Nb2 cell conditioned medium (Nb2-CM) was determined. The autocrine growth inhibitory activity of Nb2-CM was biologically characterized and attempts were made to biochemically characterize and purify the Nb2 cell autocrine growth inhibitor as well as to determine its mechanism of action. Quantitative analysis of transmission electron micrographs reveals that the ultrastructural morphology of lactogen-dependent Nb2-11c cells differs from that of lactogen-independent Nb2-SP cells. Nb2-11c cells exhibit a greater incidence and volume density of nuclear pockets, whereas the incidence and volume density of lipid droplets is greater in the Nb2-SP cell line. Surface feature of Nb2-11c and Nb2-SP cells, as examined with scanning electron microscopy, and indistinguishable. Nb2-11c and Nb2-SP cells share a common mode of growth control in the form of constitutive secretion of an autocrine inhibitory factor. Medium conditioned by either Nb2-11c or Nb2-SP cells inhibits the growth of both cell lines. Nb2-CM-mediated growth inhibition is dose-dependent and reversible. Nb2-CM does not induce quiescence or cell death, but rather, causes a delay in the progression of cells through all phases of the cell cycle. Nb2 cell proliferation stimulated by a variety of mitogens is inhibited by Nb2-CM. Nb2-CM also has the ability to inhibit the growth of normal rat splenocytes as well as MCF-7 human breast cancer cells. Biochemical analysis of Nb2-CM was equivocal; however, indirect evidence suggests that the autocrine growth inhibitory factor produced by Nb2 cells may be a prostaglandin or another arachadonic acid metabolite since the growth inhibitory activity of Nb2-CM is reduced when CM is prepared in the presence of indomethacin. Interestingly, levels of prostaglandin F₁(α) are elevated in CM-treated culture supernatants. Examination of other signal transduction systems in Nb2 cells suggests that neither cAMP activation, polyamine biosynthesis, nor protein kinase C activation mediate or influence the inhibitory effect of Nb2-CM.