Histone acetyltransferases (HATs) are important enzymes in transcriptional control and potential targets for chemotherapeutic intervention in malignant diseases. Among different HAT members, the yeast Esa1 and human Tip60 (the HIV-1 Tat interactive protein, 60KDa) play multiple roles in normal cellular processes including transcription, cell cycle and checkpoint machinery, double strand DNA break repair, apoptosis, and cell cycle progression. Tip60 is also implicated in several human diseases such as prostate cancer, and gastric cancer. These studies suggest that Tip60 is a potential therapeutic target for new cancer treatment. So, we designed experimental work to synthesize and investigate organic inhibitors of Tip60 using different strategies, including substrate analogs, small molecule screening, and modification of the natural product anacardic acid. These studies provide important chemical agents for basic biology research of HAT function, and produce potential lead compounds for future pharmacologic intervention of HAT deregulation in cancer. Currently, of the methods used for the measurement of acetyltransferase activities, many comprise tedious separation procedures and involve enzyme-coupled steps or radioactive materials. These shortcomings have limited their applications in high-throughput screening (HTS) of HAT inhibitors. To circumvent these problems, a homogenous fluorescent HAT assay based on engineered H4 peptide was designed, synthesized, and evaluated. The data showed that these fluorescent reporters can be used to detect the acetyltransferase activities.

Thesis (Ph. D.)--University of Sydney, 2007. / Title from title screen (viewed October 20, 2009) Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy to the Dept. of English, Faculty of Arts. Includes bibliographical references. Also issued in print format.

The aim of our study was to examine Swedish crime reporters’ approach to the social medium Flashback. Our main focus was the crime reporters’ use of the medium Flashback, and attitude towards their own use of media ethics in relation to the medium Flashback. Flashback as a medium is not obliged to any media ethics and lacks a legally accountable publisher. In the search for information about committed crimes people tends to visit Flashback for the information that the traditional media cannot offer. In that sense Flashback challenges the foundation of media ethics today. That is why we found it interesting to study crime reporters in particular. The study used a qualitative approach and was conducted through interviews with eight crime reporters. We have used theories about media ethics, social media and participatory journalism to explore the development in the area and how journalists have adapted to this change. The result shows that there are journalists who use Flashback as a journalistic tool to find information, to save time and as a mean to keep up-to-date. And moreover, the study gives examples on how an online forum with anonymous sources can be used – like any other source, as long as you are source-critical.

Metaphors and assumptions which underlie them occur in everyday language use, including that found in newspaper articles. Conceptions constructed by these metaphors frame how social issues are thought about and acted upon. Adult literacy is such an issue. These representations influence how readers view literacy, and, in turn, may impel policy and practice. Therefore, the purpose of this study was to examine how articles were distributed over the 1990s in eight Canadian newspapers, what metaphors predominated, which endured, along with metaphorical representations, interpreted and constructed. Using a conceptual approach within the qualitative paradigm, the method was a blend of discourse analysis and critical linguistics, using the metaphor as the unit of study. Ideas from discourse research, metaphor studies, critical linguistics, critical literacy and conceptual analysis shaped the theoretical framework. Sources for research materials were the University of British Columbia Library, in particular its microform section and its online services, the University of Victoria Library, the Vancouver Public Library, the World Wide Web, and databases. Eight Canadian newspapers provided articles relating to adult literacy. The 284 articles collected in the sample were read for instances of metaphor. Access and Excel assisted in seeing the data; the findings were distilled from resulting tables. A culminating diagram depicted the metaphorical representations of adult literacy and guided discussion. Results showed most articles were published in 1990 and 1995 in conjunction with the release dates of literacy reports and surveys. In addition, metaphorical representations, clustered under the framework of a noun as a person, place, or thing, depicted adult literacy as a complex and often contradictory conception comprised of text personified, eight distinct, contrasting places, and two concrete and twelve abstract things. As a place, literacy is represented as a nation, region, sanctuary, divide, found world, lost world, dark territory and null space. As a concrete entity, literacy is organic, a commodity, a product, or a barrier. Literacy as an abstract entity is depicted as science, a deficit, burden, medical entity, spatial entity, journey or quest, crusade or cause, aspiration or liberation, advertising campaign, condition or disability, battle or competition, or theatrical event. Five stereotypes represented the illiterate: the child, the prisoner, the other, the heroic victim and the good citizen. Finally, most metaphors endured over the ten years with literacy as science being the most prevalent and sustained. The study makes six recommendations. First, newspapers should research and publish significant findings of how they construct conceptions such as adult literacy for their readers. Next, discourse and conceptual analysis should be more widely used by adult education researchers. Thirdly, research stemming from discourse and conceptual analysis should be reviewed by adult educators when they are discussing educational program planning or curricular and policy decisions. Fourthly, adult literacy theorists and practitioners should continue to expand their knowledge of conceptions of literacy by using investigative processes including qualitative research that moves beyond functionalist views. Also, adult educators should examine significant educational conceptions and their representations in the media and compare these to the conceptions discussed in academic literature. The last recommendation is that the representations of adult literacy and the illiterate in this study should be compared with the views of adult literacy practitioners and their students.

No description available.

The relationship between the media and politicians is uncertain at best. This study examined the attitudes of Indiana state senators and representatives with regard to the way they are treated by their local media. It is based on a study conducted in 1990 by Dr. Daniel Riffe, that sought similar opinions from Alabama state lawmakers.The hypothesis assumed that legislators who indicated they found the media useful would not view them as adversarial. A 39% response rate was achieved, which while not high enough to statistically validate findings did indicate a rejection of the hypothesis, as well as reveal other interesting observations. / Department of Journalism

Thesis (M.Soc.Sc.)--University of Waikato, 2007. / Title from PDF cover (viewed May 2, 2008) Includes bibliographical references (p. 161-182)

Thesis (M.J.)--Carleton University, 2003. / Includes bibliographical references (p. 161-170). Also available in electronic format on the Internet.

Thesis (M.A. (Journalism & Media Studies)) - Rhodes University, 2008. / A thesis submitted in partial fulfilment of the requirements of the degree of Master of Arts in Journalism and Media Studies.

Inaug.-Diss.--Bern. / Bibliography: p. 88-91.