Made available in DSpace on 2014-06-11T19:32:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-02-12Bitstream added on 2014-06-13T19:42:46Z : No. of bitstreams: 1 ortolani_fa_dr_jabo.pdf: 3061947 bytes, checksum: 30bfe3f840277de1e8a0f0bcf4721c17 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Plântulas de C. antisyphilitica (Mart.) Mart., T. chrysotricha (Mart. ex Dc.) Standl., T. heptaphylla (Vell.) Toledo, T. roseo-alba (Ridl.) Sand. e Z. tuberculosa (Vell.) Bur., espécies arbóreas conhecidas popularmente como ipês, foram estudadas morfo-anatomicamente, citogeneticamente e palinologicamente. As sementes estenospérmicas apresentam testa em variados tons de castanhos com alas hialinas, assimétricas, flexíveis e de textura papirácea. As plântulas são angiospermas, dicotiledôneas, fanerocotiledonares e epigéias. Os sistemas radiculares são axiais e ramificados. Tanto hipocótilos como epicótilos são verdes e podem apresentar ou não pêlos. Os cotilédones são foliáceos, reniformes, bilobados, clorofilados, peciolados e com recortes apicais profundos. Os eófilos são simples, simétricos, glabros ou pilosos, peciolados e de filotaxia oposta. Apresentam ápice obtuso ou acuminado, base cuneada e margem serreada. As estruturas vegetais apresentam epidermes simples, unisseriadas e pilosas. Geralmente, os cotilédones, os eófilos e os metáfilos são dorsiventrais, hipoestomáticos com estômatos anomocíticos. Os mesófilos cotiledonares são heterogêneos, exceto em Tabebuia heptaphylla onde é homogêneo. O hipocótilo pode ser considerado zona de transição por apresentar elementos vasculares em diferenciação. O epicótilo possui os feixes vasculares colaterais organizados. Os elementos traqueais internos ao córtex da raiz estão dispostos em forma de anel ao redor de uma medula parenquimática. Nos pecíolos dos metáfilos os feixes vasculares colaterais dispõemse em forma de anel, com exceção de C. antisyphilitica e T. heptaphylla onde mostram-se em forma de ferradura. Todas as espécies possuem 2n = 40 cromossomos, exceto em T. chrysotricha onde foram detectadas sementes poliembriônicas com 2n = 80 cromossomos... / Seedlings of C. antisyphilitica (Mart.) Mart., T. chrysotricha (Mart. ex Dc.) Standl., T. heptaphylla (Vell.) Toledo, T. roseo-alba (Ridl.) Sand. and Z. tuberculosa (Vell.) Bur., arboreal species popularly known as ipê, were morphoanatomically, cytogenetically and palinologycally studied. The estenospermic seeds have tegument in varied chestnut tones, with hyaline, asymmetric, flexible and paper texture expansions. The seedlings are angiosperm, dicotyledonous, phanerocotilar and epigeal. The root systems are axial and ramified. Both hypocotyll and epicotyll are green and can present pile or not. The cotyledons are foliaceous, kidney shaped, bilobe, chlorophyll, petiolate and with apical deep cuttings. The eophylls are simple, symmetrical, with or without pile, petiolate and of opposite phyllotaxy. They present obtuse or acuminate apex, cuneate base and sawed margin. The vegetable structures showed simple, unisseriate, pile epidermis. Generally the cotyledons, the eophylls and metaphylls are dorsiventral, hipostomatic, with anomocytic stomatal. The cotyledons mesophyll is heterogeneous, except the Tabebuia heptaphylla, within is homogeneous. The hypocotyll can be considered a transition zone by presenting vascular elements in differentiation. The epicotyll has organized collateral vascular bunches. The windpipe elements the root cortex is arranged in a ring around a parenquimatic medulla. In the petiolates of the metaphyll the collateral vascular bunches disposed is ring shaped, except the C. antisyphilitica and T. heptaphylla, where it is shown in a horseshoe shaped. All the species have 2n = 40 chromosomes, except the T. chrysotricha, where polyembryonic seeds with 2n = 80 chromosomes were detected. The chromosomes are small and present uniformity within the same karyotype...(Complete abstract, click eletronic address below)

Drought-induced tree mortality is a phenomenon affecting many forest ecosystems and is predicted to increase under ongoing climate change. Forest stability partly depends on regeneration: the process of renewing mature forest with subsequent generations. As seedlings are more susceptible to drought effects than mature trees, mortality of the seedling bank can represent a major bottleneck controlling forest structure and species composition. Scots Pine (Pinus sylvestris L.) is the most widely distributed of the Pinus species, covering a broad latitudinal gradient of ecological conditions. The thesis aims to deepen understanding of drought-induced mortality, while analysing intra-specific variation in the phenotypic and metabolic profile of Pinus sylvestris seedlings subjected to drought stress. I also consider the relevance of the results to the broader conceptual framework of drought-induced mortality. The experiments utilise seeds from different populations of origin (provenances) across the north-south axis of the European range of Pinus sylvestris, in order to determine the extent of regeneration capacity in this species under drought. Seeds were collected from different populations (provenances) that, along with other climatic and edaphic differences, span a gradient of water availability: from wet (Scotland) to intermediate (Austria, Poland) to dry (Spain). In Chapter 2, the effects of osmotic stress on the initial seedling establishment stage were studied by comparing phenotypic responses across provenances. Seedling germination, early growth, osmotic stress tolerance and survival were investigated using a polyethylene glycol irrigation treatment as a proxy for rapid and severe drought. Treatment, provenance and interaction effects were found for rate of germination, final proportion of seeds germinated, seedling size, and superoxide dismutase activity (an antioxidant enzyme). Root investment was affected by both provenance and time to germination. Although there was no significant effect of provenance on survival, a trend towards increased probability of survival under osmotic stress was indicated for the southernmost (driest) as compared with the northernmost (wettest) provenance. Chapter 3 investigates the responses of older seedlings (at 10 months) to a drying down of soil moisture for 40 days. Morphological and physiological data were collected to assess intra-specific and intra-population variation in the seedling stress response under drought. A metabolomics analysis using Ultra performance Liquid chromatography followed by mass spectrometry (UPLC/MS) was carried out to investigate whether metabolic markers could be identified that are suggestive of heightened oxidative stress and whether populations in different climatic and edaphic environments show variation in metabolic activity under drought. Preliminary results suggest large intra-population variability yet clear differentiation in metabolic responses to drought over the time course of the experiment. Univariate and multivariate analyses indicated that among the most significant increases in response to drought were those involved in osmoprotective and antioxidant capabilities, including the free amino acid proline and a quercetin derivative (a flavonoid). Interestingly, provenances, either under experimental drought or not, did not show significantly different metabolite profiles, even though provenance and its interaction with drought treatment did significantly affect seedling biomass and photochemical efficiency. In Chapter 4 the effects of provenance, maternal parentage and seed weight on germination rate, final germination percentage, as well as seedling drought responses in biomass allocation and the expression of selected antioxidant genes were analysed. Seed weights were measured individually and seed weight was found to have a strong positive effect on: germination rate, seedling dry weights, and number of needles. Expression of two antioxidant enzymes increased under drought. Seed weight was strongly determined by provenance and maternal parentage as well as their interaction. However, root to shoot biomass allocation depended on provenance and maternal effects that were not mediated by seed weight effects. Principal component analysis indicated that the Spanish provenances could be characterised by a higher root to shoot ratio and stem weight. Specific leaf area was also found to be lowest for the Spanish provenances.

Made available in DSpace on 2014-06-11T19:23:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-11-28Bitstream added on 2014-06-13T19:29:19Z : No. of bitstreams: 1 sabonaro_dz_me_jabo.pdf: 617146 bytes, checksum: 98c187b48b5aac899fd854d8c78ca618 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este trabalho teve como objetivo avaliar o comportamento de mudas de Tabebuia heptaphylla (Vell.) Tol. (ipê-roxo), Schizolobium parahyba (Vell.) Blake (guapuruvu) e Cariniana legalis (Mart.) Kuntze (jequitibá) em diferentes substratos e níveis de irrigação e estudar utilização de diferentes quantidades de composto de lixo urbano nos substratos. O experimento foi instalado no Departamento de Engenharia Rural da Faculdade de Ciências Agrárias e Veterinárias - UNESP, Câmpus de Jaboticabal - SP. O delineamento utilizado foi o inteiramente casualizado consistindo de 30 tratamentos num esquema fatorial 15 x 2 (15 substratos e 2 níveis de irrigação), em 2 repetições. Os substratos foram constituídos de diferentes combinações: composto de lixo, Plantmax®, esterco cutido de gado, vermiculita e solo. Para acompanhar o desenvolvimento das mudas da espécie estudada, foram avaliadas as seguintes características: altura da parte aérea (H), diâmetro do colo (D), número de folhas (NF), matéria seca da parte aérea (MSPA), matéria seca do sistema radicular (MSR), matéria seca total (MST), relação altura da parte aérea/diâmetro do colo (H/D), índice de qualidade de Dickson (IDQ) e relação altura da parte aérea/matéria seca da parte aérea (H/MSPA). As características (H, D e NF) foram medidas aos 75, 90, 105, 120, 135 e 150 dias após a semeadura (d.a.s) para Tabebuia heptaphylla (Vell.) Tol. (ipê-roxo) e Cariniana legalis (Mart.) Kuntze (jequitibá) e aos 20, 35, 50 e 65 para Schizolobium parahyba (Vell.) Blake (guapuruvu). Através da análise dos resultados obtidos, concluiuse que o composto de lixo urbano favoreceu o desenvolvimento das plantas de Schizolobium parahyba (Vell.) Blake. Quanto aos níveis de irrigação testados, pôde-se verificar que houve diferenças estatísticas para todas as espécies estudadas e o nível de irrigação 150% ET se destacou em relação ao 100% ET. / The objetive of this research was to evaluate the Tabebuia heptaphylla (Vell.) Tol. (ipê-roxo), Schizolobium parahyba (Vell.) Blake (guapuruvu) e Cariniana legalis (Mart.) Kuntze (jequitibá) seedlings behavior in different substrates and irrigation levels and also to study the use of urban waste compost in the substrates. The experiment was carried out in the College of Agrarian Science, UNESP University, Department of Agricultural Engineering, Campus of Jaboticabal, São Paulo, Brazil. The experimental design used was the completely randomized with a factorial arrangement of treatments (15 substrates and 2 irrigation levels). The substrates were composed by different material combinations: urban waste, Plantmax®, tanned cattle manure, vermiculite and soil. For the study of the seedlings development, the following characteristics were evaluated: the plant height (H) and stem diameter (D), leaf number (LN), dry matter of the acre ad seedling portion (D M ASP), dry matter of root system (DMRS), total dry matter produce (TDMP), relation between plant height/colon diameter (H/D), Dickson quality index (IDQ) and (H/PMSPA). Evaluations of (H, D and NF) were made on 75, 90, 105, 120, 135 and 150 days after sowing (d.a.s) for Tabebuia heptaphylla (Vell.) Tol. and Cariniana legalis (Mart.) Kuntze and for 20, 35, 50 e 65 days after sowing (d.a.s) for Schizolobium parahyba (Vell.) Blake. Schizolobium parahyba (Vell.) Blake.

Orientador: Hélio Grassi Filho / Resumo: Após a implantação das normas para "Produção de Muda Certificada de Citros no Estado de São Paulo", no ano de 1998, houve uma grande mudança no sistema de produção de mudas cítricas, passando do sistema de produção a céu aberto para um ambiente protegido, ou produção das mudas em estufas, e isso atingiu diretamente o produtor tradicional, que produzia até então a céu aberto. Essas mudanças foram necessárias devido ao aparecimento de doenças e pragas que estavam colocando em risco a produção citrícola brasileira, hoje, a maior do mundo. A doença Clorose Variegada dos Citros (CVC), é a principal doença que desencadeou tais mudanças. Transmitida pôr um inseto vetor, exigiu que a produção de mudas fosse em ambiente telado para evitar a contaminação, que antes a céu aberto proliferava largamente. Como a maior parte da produção de mudas era feita por produtores com conhecimentos técnicos limitados, o novo sistema exigiu diversas mudanças no sistema de produção, adoção de novas técnicas, novos manejos e investimentos altos na construção das estufas. Mesmo tendo normas a serem seguidas, cada produtor adaptou-se a seu modo ao novo sistema de produção, não comprometendo com isso a qualidade. O presente trabalho analisou as influências dessas mudanças sobre o produtor, através de questionários e as respostas foram tabuladas e comparadas às Normas de Produção de Mudas. Concluiu-se neste trabalho que os objetivos principais das Normas foram atingidos com um maior controle de doenças das mudas e conhecimento da origem do material genético. A preocupação inicial com a dificuldade dos produtores se adaptarem às novas normas, não concretizou, visto que houve uma grande aceitação por parte deles, destas mudanças. / Abstract: After the standards implanting for "Produção de muda Certificada de Citros no Estado de São Paulo", in the year of 1998, there was a big change in the production system of citric seedling, passing from the production system outdoors to a protected atmosphere, or the production of the seedling in greenhouses, and this directly affected the traditional producers, which until then were to produce outdoors. Those changes were necessary due to the appearance of diseases and nuisance that were putting in risk the Brazilian citric production, considered today the biggest of the world. The disease "Clorose Variegada dos Citros" (CVC), is the main disease that unchained such changes. Transmitted by a kind of insect vector, it demanded that the seedling production was made in a greenhouse atmosphere to avoid contamination, which before this, on outdoors used to proliferate a lot. As most part of the seedling production was made by producers with limited technical knowledge, the new system demanded lots of changes in the production system, adoption of new techniques of production, new handlings and high investments to build greenhouses. Even having rules to be followed, each producer adapted in its own way to the new production system, not committing to the quality. This essay analyzed influences of those changes on the producer, through questionnaires and the answers were Tabbed and compared to the Seedling Production Directions. It was concluded in this work that the main objectives of directions were attained with a higher control on the seedling diseases and knowledge on the origin of the genetic material. The initial concern about the producers difficulty in adapting to the new directions, did not come true, once there was a high comphance to the changes by the producers. / Mestre

Orientador: Denise Maria Trombert de Oliveira / Banca: Luiz Antonio de souza / Banca: Silvia Rodrigues Machado / Resumo: Mimosa Linnaeus é o gênero com maior número de representantes de Mimosoideae no cerrado, com 189 espécies registradas. É um gênero que exibe características morfoanatômicas, hábito e tipos de frutos variados. Mimosa daleoides Benth., M. dolens Vell. var. anisitsii (Lindm.) Barneby e M. orthacantha Benth. são arbustivas e de ocorrência comum em remanescentes do cerrado paulista, observadas como invasoras, e foram selecionadas para o presente trabalho. Os objetivos foram descrever morfologia, anatomia e ontogênese dos frutos e sementes; detalhar a morfologia das plântulas e a anatomia e venação de cotilédones e eofilos; e comparar as espécies entre si e com a literatura. O material coletado foi processado segundo técnicas usuais em microscopia óptica, utilizando inclusão em metacrilato, cortes em micrótomo de rotação e coloração com azul de toluidina; também foram confeccionadas lâminas semipermanentes para análise de maceração, diafanização e cortes a mão livre. O ovário das três espécies é curto-estipitado, com 3-5 óvulos anátropos, tendendo a campilótropos apenas em M. daleoides. Emergências se formam ao longo do desenvolvimento do fruto no pericarpo; o mesocarpo é parenquimático, com hipoderme externa e interna de M. dolens var. anisitsii e somente a interna de M. daleoides com células fenólicas. Um meristema adaxial se desenvolve, do qual deriva o endocarpo externo, lignificado no final da maturação, e endocarpo interno, parenquimático. Mimosa orthacantha exibe fibras e esclereídes de orientações opostas no endocarpo externo, diferenciando-se das demais. A partir da análise anatômica, pode-se concluir que os frutos formados são craspédios em M. daleoides e M. orthacantha, ligados ao eixo da inflorescência do tipo racemo na primeira e glomérulo na segunda, e legumes de deiscência passiva em M. dolens var. anisitsii,...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Mimosa Linnaeus is the genus of Mimosoideae most represented in the cerrado, with 189 recorded species. The genus exhibits varied morphological and anatomical characteristics, habits, and fruit types. Mimosa daleoides Benth., M. dolens Vell. var. anisitsii (Lindm.) Barneby, and M. orthacantha Benth. are shrubs that commonly occur in remnant areas of cerrado vegetation in São Paulo State and are often considered invasive plants. The present work describes the morphology, anatomy, and ontogeny of the fruits and seeds of this species, examines the morphology of the seedlings, the anatomy and venation of the cotyledons and eophylls, and compares the three species and information concerning them in the published literature. The plant material was processed according to traditional techniques in light microscopy, including embedding in methacrylate, sectioning in a rotary microtome, and staining with toluidine blue. Additionally, semi-permanent slides were prepared for maceration analyses, clearing leaves, and free-hand sections. The ovary of the three species is short-stipitate, with 3-5 anatropous ovules, tending towards a campylotropous only in M. daleoides. Emergences form in the pericarp during fruit development; the mesocarp is parenchymal, with an outer and inner hypoderm in M. dolens var. anisitsii but only an inner hypoderm with phenolic cells in M. daleoides. An adaxial meristem develops, from which the outer and inner endocarp are derived; the outer is lignified at maturation, and an inner is parenchymatous. Mimosa orthacantha demonstrates fibers and sclerids oriented in opposite directions in the outer endocarp, a characteristic that differentiates this species from the others. Anatomical analyses indicate that the fruits of M. daleoides and M. orthacantha are craspedia and are fixed to the inflorescence axis of the raceme ...(Complete abstract click electronic access below) / Mestre

Orientadores: Flavio Antonio Maës dos Santos, José Luís Campana Camargo / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-17T21:49:58Z (GMT). No. of bitstreams: 1 Brum_HeloisaDantas_M.pdf: 1800057 bytes, checksum: e78dd6cce97739c55589c8fa04d0c622 (MD5) Previous issue date: 2011 / Resumo: As espécies do gênero Oenocarpus que ocorrem na Amazônia Central são O. bacaba, O. bataua e O. minor e apresentam distribuição espacial diferente ao longo do gradiente topográfico da região, caracterizado pelos habitats de baixio, vertente e platô. As plântulas das três espécies são encontradas em todos os habitats. Entretanto, os adultos de O. bataua só ocorrem nos habitats de baixio, e os de O. bacaba e O. minor só são encontrados nas vertentes e platôs. O objetivo geral deste estudo foi descrever a distribuição espacial dos estádios ontogenéticos dos indivíduos de três espécies do gênero Oenocarpus e entender como estes indivíduos são afetados pelos fatores abióticos que caracterizam os habitats de baixio, vertente e platô na Amazônia Central. Em campo foram instaladas 72 parcelas de 5x50 m nos baixios, vertentes e platôs em três reservas de floresta contínua pertencentes à Área de Relevante Interesse Ecológico do Projeto Dinâmica Biológica de Fragmentos Florestas (PDBFF), a cerca de 80 Km de Manaus, AM. Os habitat se diferenciam pelas características edáficas, principalmente textura e teor de água do solo. A abertura de dossel variou pouco entre os habitats. Foram determinados cinco estádios ontogenéticos para as três espéceis: plântula, infante, juvenil, adulto imaturo e adulto reprodutivo. A distribuição espacial das espécies não foi correlacionada com nenhuma das variáveis edáficas, exceto O. bataua, que apresentou correlação positiva com o teor de água do solo a partir do estádio de juvenil. Este estudo permite afirmar que diferenças no ambiente físico da Amazônia Central podem ser importantes em escala local para a distribuição de palmeiras. Embora somente alguns estádios ontogenéticos das três espécies tenham respondido às características edáficas, a segregação espacial entre os habitats é evidente e a relação entre cada espécie e o ambiente varia com o desenvolvimento ontogenético, como uma forma de segregação de nichos em escala local, visível nos indivíduos adultos destas espécies co-genéricas / Abstract: The species of the genus Oenocarpus occurring in Central Amazon are O. bacaba, O. bataua and O. minor. They present a distinct spatial distribution along the topographic gradient of the region, characterized by lowlands, slopes and plateaus. Seedlings of this species are found in all habitats. However, adults of O. bataua only occur in lowland habitats and, by instane, O. bacaba and O. minor are found only in the slopes and plateaus. The aim of this study was to describe the spatial distribution of individuals' ontogenetic stages of three species of the genus Oenocarpus and to understand how these individuals are affected by environmental factors that typify the habitats of lowland, slope and plateau in the Central Amazon region. A total of 72 plots of 50x5 m were set up at the three habitats of three continuous forest reserves of the Biological Dynamics of Forest Fragments Project (BDFFP), located about 80 km from Manaus, AM. The three habitats can be distinguished by some soil characteristics, especially texture and water content. The canopy cover of the trhee habitats were similar. Five ontogenetic stages were determined for the three species: seedling, infant, juvenile, immature adult and reproductive adult. The patterns of spatial distribution for the three species was not correlated with any of the soil variables, except for juveniles of O. bataua, which showed a positive correlated with water content. This study allows us to assert that differences in the physical environment of Central Amazonia may be important on a local scale for the distribution of palms trees. Although only some ontogenetic stages of three species have responded to the soil characteristics, spatial segregation amongst habitats was evident and the relationship between each species and the environment varies accordantly to the ontogenetic development, as a niche segregation form on a local scale, visible in adults of this co-generic species / Mestrado / Ecologia / Mestre em Ecologia

Este experimento avaliou, em casa de vegetação, o desenvolvimento de mudas de cafeeiro produzidas em tubetes, sob o efeito de malhas utilizadas para sombreamento. Os tratamentos utilizados foram a malha negra 50%, conhecida comercialmente como “sombrite” ou “tela preta”, considerada padrão para o desenvolvimento destas mudas, e malhas termo-refletoras com quatro diferentes percentagens de atenuação da radiação solar, sendo as malhas 40%, 50%, 60% e 70%. O delineamento experimental adotado foi em blocos casualizados contendo os 5 tratamentos, com 4 repetições, totalizando-se assim 20 parcelas. As parcelas foram representadas por 20 bancadas, cada qual contendo fechamento superior com um tipo de malha para sombreamento, e também fechamento nas laterais, atenuando a radiação solar lateral. Cada parcela foi composta por 175 mudas, sendo 161 para bordadura, e 14 para avaliações. As irrigações, realizadas diariamente, foram implementadas com a utilização de uma barra motorizada semi-automatizada que se deslocava longitudinalmente na casa de vegetação em ambos os sentidos. A lâmina desejada era ajustada de acordo com a velocidade de deslocamento da barra que era regulada por meio de um inversor de freqüência, responsável por este controle. Para monitorar o microclima, foi instalado interiormente a cada parcela um psicrômetro não ventilado e de tempos em tempos foram realizadas medidas de radiação solar global, fotossinteticamente ativa e o saldo de radiação (SR), estabelecendo-se relações de transmissividade global (TRG) e transmissividade fotossinteticamente ativa (TRFA). As variáveis fisiológicas utilizadas para avaliar o desenvolvimento das mudas foram área foliar, altura das plantas, diâmetro do caule, e, ao final do experimento, foram realizadas análises de matéria seca da parte aérea, do sistema radicular e matéria seca total. A avaliação dos dados psicrométricos mostrou que a temperatura do ar pouco variou nas parcelas, assim como a umidade relativa do ar. Com relação a TRG e TRFA, verificou-se que malhas com menor atenuação da radiação solar apresentaram em geral maior transmissividade, conforme esperado. Para a variável saldo de radiação, a presença de interferências ambientais inviabilizou a maior parte dos dados, verificando-se em apenas parte deles uma tendência de maior SR nas malhas 40% e 50%. A avaliação das variáveis fisiológicas mostrou que a área foliar não apresentou diferença significativa entre os tratamentos. Já para altura da planta e diâmetro do caule, verificou-se que as mudas produzidas sob as malhas termo-refletoras 40% e 50% foram superiores àquelas produzidas sob malha negra 50% e malhas termo-refletoras 60% e 70%, respectivamente. Nas avaliações de matéria seca, verificou-se que as mudas produzidas sob as malhas termo-refletoras 40% e 50%, e malha negra 50% acumularam mais matéria seca comparativamente àquelas produzidas sob malhas termo-refletoras 60% e 70%. Assim, nas condições em que desenvolveu-se o experimento, concluiu-se que as malhas termo-refletoras 40% e 50% e negra 50% apresentaram melhores resultados que as malhas termo-refletoras 60% e 70%. / This experiment evaluated, in greenhouse conditions, the development of coffee seedlings produced in plastic tubes, under the effect of screens used for shading. The treatments were black screen 50%, known commercially as "sombrite" or as "black screen", considered standard for the development of these seedlings, and thermo-reflective screens with four different percentages of attenuation of the solar radiation, being meshes 40%, 50%, 60%, and 70%. The adopted experimental delineation was randomized blocks with 5 treatments and 4 replicates, making up 20 parcels. The parcels were represented by 20 benches; each one with superior closing system with one type of screen, and also side closing system, attenuating the side solar radiation. Each parcel was composed by 175 seedlings, from which 161 were used as a border, and 14 used for the evaluations. The irrigation, daily carried out, was implemented with the use of a semi-automated motorized bar that dislocated longitudinally along the greenhouse in both directions. The desired water amount was set in accordance to the bar displacement speed that was established by means of a frequency inverter, responsible for this control. To monitor the microclimate, one psicrometer was installed inside each parcel and, from time to time, global solar radiation, photosynthetically active radiation, and net radiation (SR) measures were taken, establishing, this way, relations of global transmissivity (TRG) and photosynthetically active transmissivity (TRFA). The physiological variables used to evaluate the development of the seedlings were leaf area, seedlings height, and stalk diameter. Analyses on the dry matter of aerial part and root system and total dry matter were carried out at the end of the experiment. The evaluation of the psicrometers data showed that the temperature of air gently varied in the parcels, as well as the air relative humidity. Relating to TRG and to TRFA, it was verified that screens with less attenuation of the solar radiation presented higher transmissivity in general, as expected. For the variable net radiation, the presence of environment interference made most data impracticable, verifying a partial trend of higher SR in the screens 40% and 50%. The evaluation of the physiological variables showed that the leaf area did not present significant difference among the treatments. Yet, it was verified that, for seedlings height and stalk diameter, the seedlings produced under thermo-reflective screens 40% and 50% were superior to those produced under black screen 50% and thermo-reflective screen 60% and 70%. It was also verified that, for the evaluations of dry matter, the seedlings produced under thermo-reflective screens 40% and 50%, and black screen 50% comparatively accumulated more dry matter than those produced under thermo-reflective screens 60% and 70%. Thus, under the conditions where the experiment was carried out, it can be concluded that the thermo-reflective screens 40% and 50% and black screen 50% presented better results than the thermo-reflective screens 60% and 70%.

Wildfires play a significant role in many different elements of Mediterranean forest ecosystems. In recent years, prescribed fires have started being used more often as a fuel reduction tool, and also as silvicultural treatment to help the regeneration and health improvement of stands. Apart from the fact that fire may alter microsite conditions, very little is known about the impact of prescribed burning on natural regeneration or plant species renewal in Mediterranean pine forests. Likewise, knowledge about the influence of seedling predators on post-fire regeneration is still scarce. In this study, we aimed to compare the effects of seedling predation on recruitment in earlier stages after prescribed burnings in three pine stands in Central Spain: a pure stand of Pinus nigra; a mixed stand of Pinus halepensis and Pinus pinaster and a mixed stand P. nigra with P. pinaster. In situ we superficially sowed seeds from two different species. In the sowing experiment, we tested two different seed provenances (drier and more humid spanish regions) for each species. In all, 60 plots (30 burned, 30 unburned) per site, with 10 seeding units per plot and more than 20,000 seeds, were used in the whole study. Seedling predation was evaluated by replicating the seeding units inside and outside a wire cage as protection for rodents and birds. Our results showed that prescribed fires alter initial seedling predation intensity: predation was significantly higher in the seedlings grown in the plots affected by prescribed fire. The individuals sown before the fire passed showed slightly more predation than those sown after fire passage. Provenances did not appear as an important predation drive. Understanding the role of the predation associated with these treatments can help improve Mediterranean pine forest management.

Existing evidence suggested that nursery inoculation with Trichoderma harzianum and arbuscular mycorrhizal fungi (AMF) could reduce deleterious effects of biotic and abiotic stresses and improve seedling quality, fruit yield and quality of tomato (Solanum lycopersicum L.). However, studies of their combined inoculation on seedling growth, fruit yield and quality of tomato plants are not well-documented. Experiments were carried out to investigate the combined effect of T. harzianum and AMF on tomato crop performance under various conditions. When combined with a T. harzianum and AMF mixture, seaweed extract from Ecklonia maxiama inhibited AMF root colonisation of tomato seedlings. Treating seedlings with a mixture of T. harzianum and AMF reduced the incidence of Verticillium wilt in tomato grown in a nethouse at early season, with negligible effect on fruit yield. Further investigations were initiated to find out whether T. harzianum and AMF were efficient when applied as a mixture or alone, at different inoculation times. Co-inoculation with T. harzianum and AMF (Glomus mosseae) improved seedling growth and development, except when both fungi were simultaneously applied two weeks after sowing. When the seedlings were allowed to grow up until full harvest in a greenhouse, both fungal inoculants increased total yield and marketable yield, but these increases were not significant. Furthermore, inoculation with AMF increased the percentage of extra-large fruit. Field experiments conducted under commercial tomato production confirmed greenhouse studies. Inoculation of tomato with T. harzianum and AMF, either alone or in combination increased early fruit yield (four first harvesting weeks). Throughout the studies, percentage AMF root colonisation in seedlings and plants remained low, despite nursery inoculation. Field experiments investigated the effects of AMF-inoculated transplants combined with biochar-amended soils on AMF root colonisation and their resultant effects on overall crop performance and microbial community structure. Biochar had no effect on AMF root colonisation, and also when combined with AMF, it had no influence on tomato productivity. Interestingly, biochar altered the fungal community while AMF might have influenced the bacterial community such as plant-growth promoting rhizobacteria, which are associated with improved plant growth, nutrient uptake and disease control in the rhizosphere. These benefits could contribute to improved yield and fruit quality. In conclusion, although the results were variable, there was a clear indication that T. harzianum and AMF can play an important role in tomato production. / Thesis (PhD)--University of Pretoria, 2012. / Plant Production and Soil Science / unrestricted

Les réserves d’huile de la graine d’Arabidopsis thaliana sont hydrolysées par des lipases au cours de la croissance post-germinative de la plantule. Une protéine capable de fixer un inhibiteur de lipase a été identifiée à partir d’un extrait de plantules de colza. La séquence de cette protéine ressemble à celles de lipases connues. L’expression transitoire du gène orthologue d’Arabidopsis chez Nicotiana benthamiana induit l’apparition d’une activité lipase. Ces données suggèrent que cette protéine est une lipase. Une étude de la localisation in vivo de cette enzyme chez Nicotiana benthamiana indique qu’elle est localisée au niveau des peroxysomes. Chez Arabidopsis, le gène codant pour cette lipase est exprimé essentiellement lors de la croissance des plantules, quand l’hydrolyse de l’huile est maximale. L’analyse d’un mutant montre que ce gène est responsable de l’essentiel de l’activité lipase mesurée pendant la mobilisation de l’huile de réserve. Ces données suggèrent que cette lipase pourrait être impliquée dans la mobilisation des réserves lipidiques pendant la croissance post-germinative. Néanmoins, l’hydrolyse des réserves n’est pas diminuée chez le mutant. Cela pourrait être lié à une compensation par d’autres lipases. / In germinating seedlings of Arabidopsis thaliana, fat storage breakdown is initiated by lipases. A protein capable to bind to a lipase inhibitor was identified from an extract of rape seedlings and its amino acid sequence found to resemble that of known lipases. Transient expression of the Arabidopsis orthologous gene led to a 100-fold increase in lipase activity in Nicotiana bethamiana leaves. Taken together, these data strongly suggest that this protein is indeed a lipase. In vivo localization studies using a GFP fusion protein in Nicotiana benthamiana as a transcient expression host showed a peroxisomal localization. In Arabidopsis, the gene coding for this lipase was found to be mainly expressed in seedlings during fat storage breakdown. Most lipase activity was abolished in germinating seedlings of an Arabidopsis mutant for this gene. These data suggest that this lipase is likely involved in the breakdown of fat storage in germinating seedlings of Arabidopsis. However, oil mobilization was not affected in Arabidopsis mutant plants. This might suggest that the effect of the mutation could be compensated for by other lipases.