Sour cherry (Prunus cerasus L. (sin. Cerasus vulgaris Mill., Prunus vulgaris Schur)) is widely grown stonefruit tree in Lithuania. Productivity of sour cherry orchard depends on many tightly related factors. It's know that negative temperature and spring frosts are important factors determinating plant productivity. These factors must be considered in plant breeding. Risks of extreme temperatures, humidity deficiency and spring frosts increases due to climate change. Plant reaction to climate change depends on plant species, cultivar and on biotic or abiotic factors. Spring starts earlier and suddenly during past years, thus vegetation of cherries starts earlier. However, probability of plant damage to spring frosts increases due to earlier vegetation, because plants are susceptible to negative temperatures due to loss of the predictive and consequential (secondary) dormancy. The aim of the research was to evaluate DNA polymorphism of sour cherry cultivars with different resistance to spring frosts, to characterize nature of phenological phase changes, to evaluate biochemical characteristics of sour cherry resistance to spring frosts formation by analysis of changes in carbohydrate amount and composition in generative organs and fruit ovaries at different phenological phases, and to evaluate changes in gene expression levels of galactinol synthase and raffinose synthase during flower development. It was established, that specific rhythm of phenological development is... [to full text] / Paprastoji vyšnia (Prunus cerasus L. (sin. Cerasus vulgaris Mill., Prunus vulgaris Schur)) yra plačiai auginamas kaulavaisinis augalas Lietuvoje. Vyšnių sodo produktyvumas priklauso nuo daugelio tarpusavyje susijusių veiksnių. Oro temperatūra yra vienas svarbiausių vyšnių paplitimą ir jų derlių lemiantis veiksnys. Žinoma, kad neigiamos temperatūros ir pavasario šalnos yra svarbus veiksnys, į kurį reikia atsižvelgti vykdant augalų selekciją. Keičiantis klimatui, didėja ekstremalių temperatūrų ir drėgmės deficito pavojus bei pavasario šalnų rizika (Augspurger, 2013). Augalų reakcija į klimato kaitos pokyčius priklauso nuo augalo rūšies ir veislės bei abiotinių ir biotinių veiksnių. Pastaraisiais metais pavasaris ateina greičiau ir staigiai. Tai paveikia vyšnias – jų vegetacija prasideda anksčiau. Anksti pradėjus vegetuoti išauga tikimybė, kad augalus pažeis pavasario šalnos. Lietuvoje nuostolių padaro vėlyvos pavasario šalnos, kurių metu pažeidžiami žiedai ir vaisių užuomazgos. Tuo metu augalai jau būna išėję iš būtinosios ir priverstinės ramybės ir jautrūs neigiamoms temperatūroms. Tyrimų tikslas buvo įvertinti skirtingo atsparumo šalnoms vyšnios veislių DNR polimorfizmą, fenologinių tarpsnių kaitos pobūdį, atskleisti paprastosios vyšnios atsparumo pavasario šalnoms formavimosi biochemines ypatybes ištiriant bendro angliavandenių kiekio ir jų sudėties kitimą skirtinguose fenologiniuose tarpsniuose esančių augalų generatyviniuose organuose ir vaisių užuomazgose, nustatyti... [toliau žr. visą tekstą]

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<p>Groundwater is an important water resource to be used to supplement the water demand for the City of Cape Town for present and future generations. Understanding the groundwater occurrence of the Table Mountain area is very important for future groundwater exploitation and management. Apart from the sea in the west, Table Mountain is mostly surrounded by the unconsolidated sediments including the Kirstenbosch, Newlands, and Oranjezicht areas. These areas are rich in groundwater resources, like springs / some of them were utilized, others not. However, there are few studies that focused on spring resource in this area. No up to date information is available for spring resources research and relative data is lacking from local research institutions. In fact, some of the spring resources in the Table Mountain area had been extracted and been utilized for local community for many years. Data and information newly obtained from this study about such groundwater resources will help future groundwater development and management. There are at least 13 springs in the selected study area. Those springs were investigated for groundwater occurrence, because spring is an important manifestation of the underlying aquifer through which groundwater dynamics can be detected. The main objective of the study was to sketch a clear picture of groundwater occurrence and to obtain an improved understanding of how geomorphology affects groundwater flow, its manifestation and quality. Water resources management is also important because this kind of water resource can be used to help meet the water demand of this local area in the future. There is relationship between the topographical features of the Table Mountain and spring occurrence. The research area delineated is used to interpret the relationship. Hydro-geochemical analysis is carried out to indicate the chemical components of the groundwater and to understand the groundwater type and water quality of this particular area. Based on the completed analysis and interpretation of factors influencing discharge and recharge, some good results were obtained and useful information is made available for first time.</p>

Wheat midge, Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae), is a key pest of wheat, Triticum aestivum L. (Poaceae), in the Canadian Prairies. The larvae destroy wheat kernels, resulting in reduction of quality and quantity of wheat. Deployment of antixenotic wheat lines, which suppress oviposition of wheat midge, can reduce damage in wheat fields. The objectives of this thesis were to explore the interactions between wheat midge and spring wheat with emphasis on oviposition behavior and to explore the antixenosis of wheat to oviposition from the point of view of genetics and crop breeding. In this research, a doubled-haploid spring wheat population was studied, which was the progeny of a cross between a susceptible wheat cultivar ‘Roblin’ and a resistant (antixenotic and antibiotic) wheat line ‘Key 10’. Oviposition of wheat midge on wheat spikes in the laboratory was affected by visual and chemical cues. The visual contrast between wheat spikes and the background color in the laboratory was important in modifying oviposition of wheat midge on wheat spikes. Low contrast resulted in low egg density on wheat spikes in the laboratory. The egg density on wheat spikes in the laboratory decreased when the background color of the spikes was red or black; while yellow and blue backgrounds did not decrease egg density on the spikes. The laboratory study provided evidence that wheat midge oviposition was affected by volatiles emitted by wheat spikes. The volatiles of spikes of a post-anthesis susceptible wheat cultivar, ‘Roblin’, and a pre-anthesis resistant wheat line, ‘Key 10’, significantly suppressed the oviposition of wheat midge in the laboratory. It is hypothesized that these volatiles might be a factor in antixenosis of wheat against wheat midge in the doubled-haploid population studied. It is suggested that the differences of oviposition behavior in susceptible and antixenotic wheats, which was observed in the laboratory, might be due to volatiles emitted by wheat spikes. However, other factors such as tactile cues might also be involved. The observation of oviposition behavior in the laboratory on the susceptible wheat cultivar ‘Roblin’ showed that wheat midge started ovipositing sooner, stayed longer, laid more eggs and left the spike sooner after the last oviposition than on the antixenotic line ‘Key 10’. However, the time required for laying one egg was similar when wheat midge was on the susceptible or resistant wheat. The observed antennation behavior of wheat midge while probing the wheat spike might indicate that wheat midge probed for chemical cues emitted by the host plant. The observed ovipositor tapping and dragging on the wheat spike surface while probing the spike suggested that there might be receptors at the tip of the ovipositor which receive tactile cues from the plant surface, guiding oviposition. The correlations between morphological traits of bread wheat spikes and antixenosis in the laboratory were not high enough to conclude that those traits were associated with antixenosis. However, more research on fine scale morphological traits of the spike may reveal relationships with antixenosis. Based on data from a laboratory trial and trials in the field over two field seasons, it was concluded that the antixenosis to wheat midge in the doubled-haploid population was probably conferred by two genes with complementary interactions among genes, and a heritability of 67%. In the two field seasons, the least preferred line received 13% and 11% as many eggs as on ‘Roblin’; ‘Key 10’ received 57% and 20% as may eggs as on ‘Roblin’. Our study did not provide evidence for linkage between antixenosis genes and the antibiosis gene, Sm1, which is associated with death of larvae of wheat midge. The antixenosis of spring wheat against wheat midge can be considered as a promising mechanism for suppressing wheat midge oviposition in the field. More research is required to reveal additional genetic information which would help crop breeders in production of cultivars antixenotic to wheat midge.

This thesis is the first indepth analysis of the jian, or advisory speeches, within a relatively unknown text, the Yanzi Chunqiu. It examines the way the Chunqiu period advisor Yanzi employs rhetorical techniques within the jian to change the conduct of his ruler Duke Jing according to Yanzi's three key political views: that the welfare of the common people is essential for the well-being of the state, that li 礼 (or ritual propriety) is of central importance in administering the state, and that the correct relationship between the minister and the ruler is he 和 (a complementary one). This study situates jian, ministerial responsibilities and Yanzi's own political views within the political and intellectual context of the Chunqiu period. It also disagrees with several scholars who claim that the text is unlikely to be a true record of this period. The rhetorical techniques within the jian (the majority of which are translated for the first time into English in this thesis), categorised according to whether they are typical (analogy, citation and chain reasoning) or atypical (provocation, song, lying and threatening resignation), are then analysed. The thesis finds that Yanzi's use of citations, analogies and chain reasoning confirms much existing research on Chinese rhetoric about the application of such techniques. However, the discoveries of Yanzi's atypical use of jian, as well as his frequent reference to Duke Huan as a historical model and his use of possible negative consequences to instill fear in his ruler, indicate that the present understanding of jian by several Western scholars largely based on the Zuozhuan and the Guoyu presents only a partial understanding of jian. The thesis suggests that more attention should be paid to lesser-known texts such as the Yanzi Chunqiu for a clearer picture of the rhetoric of this period.

The City of Georgetown, Kentucky relies on a vast karst spring network as a drinking water source. This karst feature has several inputs from sinkholes and streams in the Cane Run Watershed: a watershed associated with a variety of land uses in the recharge area. The recharge area encompasses the area from North Lexington to Georgetown and is composed of urban, suburban, agricultural and industrial usage. A serious water quality issue exists with respect to the impact of fecal contamination within the spring recharge area. Identification of fecal contamination is quantified by microbial indicators adapted from surface water applications: fecal load (E. coli), fecal source (two human-host specific Bacteroides DNA markers) and fecal age (AC/TC ratio). These three criteria are used in a categorical Microbial Source Tracking (MST) model to assign a Sanitary Category Value (SCV) between 0 and 3 for each sample location. Low SCVs (1.5) are associated with high values of fecal load, low fecal age and detectable concentration of human-specific markers. SCV measured during dry weather conditions are indicative of potentially leaking human sewers. Due to retention and conservation of fecal load (E. coli) and age (AC/TC) microbial indicators in the karstic environment, ambiguous SCV model results cannot pinpoint, with statistical confidence, fecal sources in a karstic environment. Human-host specific genetic markers (HF183 and HuBac) were also detected at all sample sites above limits of detection, indicating steady inflow of fecal material during all sample events. By adding a flow multiplier and expressing HF183 and HuBac values as a load, it was strongly indicated that a human fecal source was entering the groundwater conduit and impacting Royal Spring independent from other upstream fecal sources. Interpretation of these trends, while strongly indicated, cannot be supported with statistical evidence.

Pavement damage due to heavy loads on thaw weakened flexible pavements is a major concern for road agencies in Western Canada. To protect weaker, low volume roads, agencies impose spring weight restrictions (SWR) during the spring thaw to reduce pavement damage. While SWR may be cost effective for highway agencies, reducing the spring weight allowances can have a major impact on truck productivity and shipping costs. Therefore an improved process that links SWR loads to pavement damage, and based on limiting failure strain, is required. This thesis developed Local mechanistic-empirical damage models to predict fatigue and rutting failure on two spring weight restricted (SWR) flexible pavements in Manitoba. The Local damage models were used to assess the SWR loads that regulate commercial vehicle weights in Manitoba based on a limiting strain relationship between truck loads and damage. The Local damage models and a calibrated Finite Element Model (FEM) were used to predict the equivalent single axle load (ESAL) repetitions to fatigue and rutting failure at varying B-Train axle loads at the Manitoba sites. The Local model predictions were compared to predictions from the Asphalt Institute (AI) and Mechanistic Empirical Design Guide (MEPDG) damage models. The results of the analysis showed that for each 1% increase in load, there was a corresponding 1% increase in strain, and up to 3% decrease in ESAL repetitions to failure, depending on the Local, AI, or MEPDG damage models. The limiting failure strains, computed from the Local model for design ESALs of 100,000, were 483μm/m and 1,008μm/m for fatigue and rutting failure, respectively. For the Manitoba sites, the predicted FEM strains at B-Train normal and SWR loads were higher than the Local model limiting strains. Therefore the Manitoba ii SWR loads regulating B-Train operations on the two pavements during the spring period appeared to be reasonable. It is recommended that the research findings be verified with further calibration and validation of the Local damage model using a larger data set of low volume flexible pavements. A strain-based concept on how to manage the SWR regime in Manitoba based on the limiting strains was developed and presented.

Wheat midge, Sitodiplosis mosellana (Géhin) (Diptera: Cecidomyiidae), is a key pest of wheat, Triticum aestivum L. (Poaceae), in the Canadian Prairies. The larvae destroy wheat kernels, resulting in reduction of quality and quantity of wheat. Deployment of antixenotic wheat lines, which suppress oviposition of wheat midge, can reduce damage in wheat fields. The objectives of this thesis were to explore the interactions between wheat midge and spring wheat with emphasis on oviposition behavior and to explore the antixenosis of wheat to oviposition from the point of view of genetics and crop breeding. In this research, a doubled-haploid spring wheat population was studied, which was the progeny of a cross between a susceptible wheat cultivar ‘Roblin’ and a resistant (antixenotic and antibiotic) wheat line ‘Key 10’. Oviposition of wheat midge on wheat spikes in the laboratory was affected by visual and chemical cues. The visual contrast between wheat spikes and the background color in the laboratory was important in modifying oviposition of wheat midge on wheat spikes. Low contrast resulted in low egg density on wheat spikes in the laboratory. The egg density on wheat spikes in the laboratory decreased when the background color of the spikes was red or black; while yellow and blue backgrounds did not decrease egg density on the spikes. The laboratory study provided evidence that wheat midge oviposition was affected by volatiles emitted by wheat spikes. The volatiles of spikes of a post-anthesis susceptible wheat cultivar, ‘Roblin’, and a pre-anthesis resistant wheat line, ‘Key 10’, significantly suppressed the oviposition of wheat midge in the laboratory. It is hypothesized that these volatiles might be a factor in antixenosis of wheat against wheat midge in the doubled-haploid population studied. It is suggested that the differences of oviposition behavior in susceptible and antixenotic wheats, which was observed in the laboratory, might be due to volatiles emitted by wheat spikes. However, other factors such as tactile cues might also be involved. The observation of oviposition behavior in the laboratory on the susceptible wheat cultivar ‘Roblin’ showed that wheat midge started ovipositing sooner, stayed longer, laid more eggs and left the spike sooner after the last oviposition than on the antixenotic line ‘Key 10’. However, the time required for laying one egg was similar when wheat midge was on the susceptible or resistant wheat. The observed antennation behavior of wheat midge while probing the wheat spike might indicate that wheat midge probed for chemical cues emitted by the host plant. The observed ovipositor tapping and dragging on the wheat spike surface while probing the spike suggested that there might be receptors at the tip of the ovipositor which receive tactile cues from the plant surface, guiding oviposition. The correlations between morphological traits of bread wheat spikes and antixenosis in the laboratory were not high enough to conclude that those traits were associated with antixenosis. However, more research on fine scale morphological traits of the spike may reveal relationships with antixenosis. Based on data from a laboratory trial and trials in the field over two field seasons, it was concluded that the antixenosis to wheat midge in the doubled-haploid population was probably conferred by two genes with complementary interactions among genes, and a heritability of 67%. In the two field seasons, the least preferred line received 13% and 11% as many eggs as on ‘Roblin’; ‘Key 10’ received 57% and 20% as may eggs as on ‘Roblin’. Our study did not provide evidence for linkage between antixenosis genes and the antibiosis gene, Sm1, which is associated with death of larvae of wheat midge. The antixenosis of spring wheat against wheat midge can be considered as a promising mechanism for suppressing wheat midge oviposition in the field. More research is required to reveal additional genetic information which would help crop breeders in production of cultivars antixenotic to wheat midge.

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